IVF0023449 (gene) Melon (IVF77) v1

Overview


Name	IVF0023449
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Calcium-binding EF-hand family protein
Location	chr03: 6859534 .. 6860374 (-)
RNA-Seq Expression	IVF0023449
Synteny	IVF0023449

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACGTATTATATATTTTGGTAATAGTCTTTCTTGTGTGTTTACATTTCAATTCTAAACCGAAGAACAAAAAGCACCATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTACAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAAAAAAGAAAAGGAAAAAAAAAAAAAAAATCCCCTTTTTTGTTATATTGTTGTTAAATATAGATATTCGTTTGGTCTGTTTATTTGCAGGTACAATTTGAGAAGCTGGGAGGCTTCAAGAATAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGTTGCTCCAATCAGATTATGTATTTCCACTCTCCTCTATAATAGAATTTTTTTTTTAGGATCAATGACTATGGTCTAATTCTGTGTCTTTGTATATGGGCAC

mRNA sequence

CACGTATTATATATTTTGGTAATAGTCTTTCTTGTGTGTTTACATTTCAATTCTAAACCGAAGAACAAAAAGCACCATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTACAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAAAAAAGAAAAGGAAAAAAAAAAAAAAAATCCCCTTTTTTGTTATATTGTTGTTAAATATAGATATTCGTTTGGTCTGTTTATTTGCAGGTACAATTTGAGAAGCTGGGAGGCTTCAAGAATAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGTTGCTCCAATCAGATTATGTATTTCCACTCTCCTCTATAATAGAATTTTTTTTTTAGGATCAATGACTATGGTCTAATTCTGTGTCTTTGTATATGGGCAC

Coding sequence (CDS)

ATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTACAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG

Protein sequence

MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT

Homology

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BLAST of IVF0023449 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.4e-28
Identity = 74/156 (47.44%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 4   ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 123
           Y      +KG  K        D   D+ E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKN------IDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMML 159
            + +++    C  MI+ +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAW--DCGRMIRVHDKNLDGFVDFEEFKNMIL 153

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BLAST of IVF0023449 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 6.8e-20
Identity = 60/155 (38.71%), Postives = 86/155 (55.48%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFL------EKPSLDFDEFLFF 68
           LHR+F   DKN DG + ++EL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 128
           ++++ +       GG   C     E D E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            +A   E++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

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BLAST of IVF0023449 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 9.9e-19
Identity = 62/159 (38.99%), Postives = 91/159 (57.23%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG + ++EL   L R+G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQNKGECKGGVAGCVQDD----HEDDTEIVYMAFKVFDMNGDGFISCDELENVLV 128
           ++++     G   GG  G  +DD     E+++++   AFKVFD NGDGFIS  EL+ VL 
Sbjct: 90  HKTLDDSFFGGACGG--GENEDDPSSAAENESDLA-EAFKVFDENGDGFISARELQTVLK 149

Query: 129 RLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           +L L +    E++    MI + D N DG++DF EFKNMM
Sbjct: 150 KLGLPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

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BLAST of IVF0023449 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.9e-17
Identity = 61/168 (36.31%), Postives = 85/168 (50.60%), Query Frame = 0


Query: 4   ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEK------PSLDFD 63
           + S +L ++FK  DKN DG +  +EL       GI +  +EL++ ++K        +D +
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYESI--------SKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFIS 123
           EF   Y SI        + +      GG  G   D+ E   E    AF VFD NGDGFI+
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMRE----AFNVFDQNGDGFIT 120

Query: 124 CDELENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            DEL +VL  L L     +  D CR MI   D + DG++DF+EFK MM
Sbjct: 121 VDELRSVLSSLGLKHGRTA--DDCRRMISMVDADGDGRVDFKEFKQMM 162

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BLAST of IVF0023449 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.2e-17
Identity = 57/164 (34.76%), Postives = 91/164 (55.49%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG++ + EL   L+ +G++  + ELES ++         L F++F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMA---------FKVFDMNGDGFISCDEL 128
           ++S++     +      G  +DD+E+D     ++         FKVFD +GDG+IS  EL
Sbjct: 101 HQSLN-----DSYFAYGGEDEDDNEEDMRKSILSQEEADSFGGFKVFDEDGDGYISAREL 160

Query: 129 ENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           + VL +L    +  SE+D    MI + D+N DG++DF EFK+MM
Sbjct: 161 QMVLGKLGF--SEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

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BLAST of IVF0023449 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 6.1e-88
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

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BLAST of IVF0023449 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 6.1e-88
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]

BLAST of IVF0023449 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.4e-79
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]

BLAST of IVF0023449 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-66
Identity = 131/158 (82.91%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           WDA++SEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of IVF0023449 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 5.0e-66
Identity = 132/161 (81.99%), Postives = 142/161 (88.20%), Query Frame = 0


Query: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFL F
Sbjct: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63

Query: 63  YESISKQNKGECKGGVAGCVQDDHED---DTEIVYMAFKVFDMNGDGFISCDELENVLVR 122
           YES+SKQNKGECKGG+     D+HED   D EIVYMAFKVFD+NGDGFISC+ELENVL R
Sbjct: 64  YESMSKQNKGECKGGLV----DEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSR 123

Query: 123 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           LELWDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 LELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

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BLAST of IVF0023449 vs. NCBI nr
Match: XP_008441608.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 putative calcium-binding protein CML44 [Cucumis melo var. makuwa])

HSP 1 Score: 332 bits (850), Expect = 9.23e-115
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]

BLAST of IVF0023449 vs. NCBI nr
Match: XP_004149438.1 (probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetical protein Csa_018262 [Cucumis sativus])

HSP 1 Score: 304 bits (778), Expect = 1.01e-103
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]

BLAST of IVF0023449 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 293 bits (750), Expect = 2.24e-99
Identity = 142/161 (88.20%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MS IS  DLHRIF KLDKN DGL+CLQELKWL DRIGIQLTMEELESFLEKPSLDF+EFL
Sbjct: 11  MSSISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFL 70

Query: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGV GC+QDDHE++ EIVYMAFKVFDMNGDGFISCDELENVL RL
Sbjct: 71  FFYESISKQNKGECKGGV-GCLQDDHEEEMEIVYMAFKVFDMNGDGFISCDELENVLSRL 130

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161
           ELWDA+R+++DYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 131 ELWDANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 170

[Close]

BLAST of IVF0023449 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 262 bits (669), Expect = 3.53e-87
Identity = 132/158 (83.54%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 160
           WDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of IVF0023449 vs. NCBI nr
Match: XP_022939513.1 (probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 261 bits (666), Expect = 1.01e-86
Identity = 131/158 (82.91%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 160
           WDA++SEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of IVF0023449 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 127.5 bits (319), Expect = 9.8e-30
Identity = 74/156 (47.44%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 4   ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 123
           Y      +KG  K        D   D+ E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKN------IDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMML 159
            + +++    C  MI+ +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAW--DCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of IVF0023449 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 98.6 bits (244), Expect = 4.9e-21
Identity = 60/155 (38.71%), Postives = 86/155 (55.48%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFL------EKPSLDFDEFLFF 68
           LHR+F   DKN DG + ++EL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 128
           ++++ +       GG   C     E D E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            +A   E++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of IVF0023449 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 94.7 bits (234), Expect = 7.0e-20
Identity = 62/159 (38.99%), Postives = 91/159 (57.23%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG + ++EL   L R+G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQNKGECKGGVAGCVQDD----HEDDTEIVYMAFKVFDMNGDGFISCDELENVLV 128
           ++++     G   GG  G  +DD     E+++++   AFKVFD NGDGFIS  EL+ VL 
Sbjct: 90  HKTLDDSFFGGACGG--GENEDDPSSAAENESDLA-EAFKVFDENGDGFISARELQTVLK 149

Query: 129 RLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           +L L +    E++    MI + D N DG++DF EFKNMM
Sbjct: 150 KLGLPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of IVF0023449 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 85.5 bits (210), Expect = 4.3e-17
Identity = 54/156 (34.62%), Postives = 83/156 (53.21%), Query Frame = 0


Query: 8   DLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEK------PSLDFDEFLF 67
           +L R+F+  DKN DG +  +EL   L  +GI +  +EL   +EK        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLE 127
            Y++I                 D+ +++ E +  AF VFD NGDGFI+ DEL+ VL  L 
Sbjct: 65  LYKTI----------------MDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLG 124

Query: 128 LWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           L       +D C+ MI+  D + DG+++++EF+ MM
Sbjct: 125 L--KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of IVF0023449 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.8e-16
Identity = 52/160 (32.50%), Postives = 83/160 (51.88%), Query Frame = 0


Query: 4   ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEK------PSLDFD 63
           I   +L R+F+  DKN DG +  +EL   L+ +GI +  ++L   + K        +D D
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 64  EFLFFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVL 123
           EF   Y SI  ++             +D E + E +  AF VFD +GDGFI+ +EL++V+
Sbjct: 121 EFESLYSSIVDEH------------HNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVM 180

Query: 124 VRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
             L L       +D C+ MI   D + DG+++++EF  MM
Sbjct: 181 ASLGL--KQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

The following BLAST results are available for this feature:

BLAST of IVF0023449 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	1.4e-28	47.44	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9FI19	6.8e-20	38.71	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q9SVG9	9.9e-19	38.99	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q2QVI1	1.9e-17	36.31	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
P43187	4.2e-17	34.76	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]

BLAST of IVF0023449 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3B6U1	6.1e-88	100.00	Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B3T2	6.1e-88	100.00	probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P...	[more]
A0A0A0KFQ6	1.4e-79	89.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1	[more]
A0A6J1FLU5	1.3e-66	82.91	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1K1Y7	5.0e-66	81.99	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893...	[more]

BLAST of IVF0023449 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008441608.1	9.23e-115	100.00	PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 put...	[more]
XP_004149438.1	1.01e-103	89.70	probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetica...	[more]
XP_038885742.1	2.24e-99	88.20	probable calcium-binding protein CML44 [Benincasa hispida]	[more]
XP_023551499.1	3.53e-87	83.54	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]
XP_022939513.1	1.01e-86	82.91	probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putati...	[more]

BLAST of IVF0023449 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	9.8e-30	47.44	Calcium-binding EF-hand family protein	[more]
AT5G44460.1	4.9e-21	38.71	calmodulin like 43	[more]
AT4G20780.1	7.0e-20	38.99	calmodulin like 42	[more]
AT1G05990.1	4.3e-17	34.62	EF hand calcium-binding protein family	[more]
AT2G43290.1	2.8e-16	32.50	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 8..36 e-value: 0.011 score: 24.9 coord: 132..160 e-value: 0.01 score: 25.0 coord: 92..120 e-value: 0.037 score: 23.2
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 12..30 e-value: 0.029 score: 13.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 94..157 e-value: 2.3E-10 score: 40.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 128..161 score: 13.592857
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 12.365459
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 11.863342
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 96..157 e-value: 4.39492E-12 score: 56.0169
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 8..60 e-value: 2.85979E-7 score: 43.3053
None	No IPR available	GENE3D	1.10.238.10		coord: 5..66 e-value: 8.7E-9 score: 37.4
None	No IPR available	GENE3D	1.10.238.10		coord: 67..157 e-value: 5.7E-19 score: 70.2
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..160
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 1..160
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0023449.2	IVF0023449.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding