Tan0000233 (gene) Snake gourd v1

Overview
NameTan0000233
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionGrowth-regulating factor
LocationLG08: 438945 .. 441656 (+)
RNA-Seq ExpressionTan0000233
SyntenyTan0000233
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGGAGGAAGAAACAGATTCCCATTTACTGCTGCTCAGTGGCAAGAGCTGGAGCACCAAGCCCTCATCTTCAAGTATATGGTCTCCGGCATCCCTGTTCCTCCCGATCTGCTCTTCACCATTAAGAGAACCTCTTTGGATTCCCCTTTCATTTCCAGGCTCTTTCCTCACCAATATCCAGACGGTAACTTATAACTTCCCATTCCTATCTTCTTATTGCTCTGTTTTCATCCATATTTAACATTGTTTTTATGTTGTGTATGCAGTTGGATGGAACTACTTGCAAATGGGTTCGGGCAGGAAAATCGACCCAGAGCCTGGAAGGTGCAGAAGAACAGACGGCAAAAAATGGAGATGCTCCAAAGAGGCATACCCAGATTCTAAGTATTGTGAGAGACATATGCACAGAGGCAAAAACCGTTCAAGAAAGCCTGTGGAAGTTTTGAAACCAACAACAACAATAAGTAATGCTCTAAACTCTAATCCATCCACCCCAGCCATCTCCTCCATCACCCAGAACTCTACTCTCTCCTCTTCTTCTCATTCCCTTGCTTCCCTCAGCACCTCATTACCCTCTGAAACTCATACCCAACACCTCCACCATTCTTGTTTCAATACTCATCCTCCCCACCCTTTTCTCTACCAACATGCCTCTTCTTCTAGGCCCCCTGGTTCTGCTCATTTGCAGCAAAACTCCAACACCTCTTCCTTGCTTCTTGACCCAGGTTCTTACTCCCACAACAACCCAGATTACAGGTTTGTACAAAAAAAAAATCTATCTATAGTTCTTTCTGTTTCTGAGATTTGTTTGGAGGTTAAACTGATGATTTTACCTTTTCTGTAACTGAGAAGAAGAAACAGGTATGTTTATGGGGTGAAGGAAGATGTTGATGAGCATCCCTTTCTGTCGGAAGAACACTCTGGGAACATGAGAGGCTTCTCTGCTTCATCTATGGAAGATTCCTGGCAGCTGACTCCTCTGACAATGAGTTGCTCTTCCGCATCCTCCAGACACAAGAACTGCTCTGCTTTACAAGGTGACTACTCTTCCTACTTGCAGCTTCAGAGCTTTGGCGATTCCCCTAAGCAACTCAAGCAAGAGGAGCATTACTACGCGCTAGGAAAATATAACGAGATGCAGAGTAAGATGGAAAGAGAGGAACCACAGAAGACTATGCATCACTTCTTTGATGAATGGTCTCCAAAAGACAGAGAATCGTGGCTTGATTTGGATGATAAGCCATCCAATACTGGCTCAGTCTCTGCAACTCGGCTTTCAATGTCTATTCCTAACTCATCGCATTCGCAGCATGACTTCTCCTCCATCTTTAGTTCGAACAAGCATAACGAAAACTGAATTGGGTTTTAAAATCCTGGTTCCTTTTTATCCAATCCATTGTGCATTTAGGATTTTGAGGAAGGAAAAACATTTTGCCTTGTAATTGGGATCCATGACCCAGCTGTTACCAAAATGCATTACAAATAAGTTAGTTAGTTTGTTTGTTTGATTTTCTTAGACGACCCACACAGCTGTGATTTCTGATTTCTGACTGATGTGTCCAAAAATGTACTTAAGTGAGAGAGAAATGACTACTGTGTTGCATGAGGTGGATTTGTTTGCATGTGTGATGAAGTTGAAGTTGAAGTTGAACAGAACAGTGGCTGGTTCCATAAAAAGGATCCAAAGTGTGTGTTTTGTTCGATTGCGGGTGTTCCCATCCACTTCACTGTATAAATAGTTAGGTGATGAATGATGAGTCAGAGAGGTGTGATAAATATAAAATGCAAAGGTAAGCAAGCAAAGCATCCTGGCAGGCATCTGTCATCTGTGTTCTGGGCTTCGTTCAAGCTTCTATGTCTGTTGTCTTGTAATAATAATAACAATAATGGGACTGTGAGAGGGCCCCCAATGGACCACGTTGCTTGGTGTTTTGGTTGCTGCTCAGACTCGGCCCTTTCTTTAGACATCACCGCTGTAAAACCCAGCCTAGTCCATATATAGTGTATAGAATAGATGCCTCTCCTCTCTTCTCTTCTCTTCTCTTCTCGTTCTCGTTTTGCTGCAAACAACCAAGGAAAAAGGACGATCATCAGCATTCATTATTCACTTAACACTTCATTTCATTTAACATTAACATTTAACGTATGCCCATCTCTGGAAAACACAAAGGGGATATTCTTGTCTTCTTTATTAACTTCTTTTCATTTCAACTCATTCAAAGTTGATTTTAAGCTGATGATATTGATTCAGATCGCTGGAATCAGTGGGAGAAAAAGAAAGCGGACAAAGGTCTTTTTTTTAGAGTGATAGGATATGGAAACCGGCCCCAATCTAAGACAAGACAACTTGCCTGCCTTTCTAATTGACAAAAATGTATCTTTCTCTTCTGTTTTTTTTCTCTTAAGGCAACTGTTTTCATTCATTTTGCTTGTGACCCTTTTCACTTTACTTGTAGTTTCATGCTAAAATGGAGCCAAGTTGGTAACTTGTTACGATGCAAATGTAGGACTAGGACAGGAAGAGACAGAGTGAGAGAGGATTGTCTCTATTTCTCTTATTTGTGAAAATGAAATTCTGGAACAAGAAAAGGGAGGGAAAAAGCTACTTTAAAGTTTAACCATGTTGATTTGATTGACAGTTTTTAGAACGATTATTTGAAGCTCTATATCCTATAAAGGCCTCAATTCAGAAAGTGCTTAAGAAGTTAA

mRNA sequence

ATGAGCGGAGGAAGAAACAGATTCCCATTTACTGCTGCTCAGTGGCAAGAGCTGGAGCACCAAGCCCTCATCTTCAAGTATATGGTCTCCGGCATCCCTGTTCCTCCCGATCTGCTCTTCACCATTAAGAGAACCTCTTTGGATTCCCCTTTCATTTCCAGGCTCTTTCCTCACCAATATCCAGACGTTGGATGGAACTACTTGCAAATGGGTTCGGGCAGGAAAATCGACCCAGAGCCTGGAAGGTGCAGAAGAACAGACGGCAAAAAATGGAGATGCTCCAAAGAGGCATACCCAGATTCTAAGTATTGTGAGAGACATATGCACAGAGGCAAAAACCGTTCAAGAAAGCCTGTGGAAGTTTTGAAACCAACAACAACAATAAGTAATGCTCTAAACTCTAATCCATCCACCCCAGCCATCTCCTCCATCACCCAGAACTCTACTCTCTCCTCTTCTTCTCATTCCCTTGCTTCCCTCAGCACCTCATTACCCTCTGAAACTCATACCCAACACCTCCACCATTCTTGTTTCAATACTCATCCTCCCCACCCTTTTCTCTACCAACATGCCTCTTCTTCTAGGCCCCCTGGTTCTGCTCATTTGCAGCAAAACTCCAACACCTCTTCCTTGCTTCTTGACCCAGGTTCTTACTCCCACAACAACCCAGATTACAGGTATGTTTATGGGGTGAAGGAAGATGTTGATGAGCATCCCTTTCTGTCGGAAGAACACTCTGGGAACATGAGAGGCTTCTCTGCTTCATCTATGGAAGATTCCTGGCAGCTGACTCCTCTGACAATGAGTTGCTCTTCCGCATCCTCCAGACACAAGAACTGCTCTGCTTTACAAGGTGACTACTCTTCCTACTTGCAGCTTCAGAGCTTTGGCGATTCCCCTAAGCAACTCAAGCAAGAGGAGCATTACTACGCGCTAGGAAAATATAACGAGATGCAGAGTAAGATGGAAAGAGAGGAACCACAGAAGACTATGCATCACTTCTTTGATGAATGGTCTCCAAAAGACAGAGAATCGTGGCTTGATTTGGATGATAAGCCATCCAATACTGGCTCAGTCTCTGCAACTCGGCTTTCAATAAGTTAA

Coding sequence (CDS)

ATGAGCGGAGGAAGAAACAGATTCCCATTTACTGCTGCTCAGTGGCAAGAGCTGGAGCACCAAGCCCTCATCTTCAAGTATATGGTCTCCGGCATCCCTGTTCCTCCCGATCTGCTCTTCACCATTAAGAGAACCTCTTTGGATTCCCCTTTCATTTCCAGGCTCTTTCCTCACCAATATCCAGACGTTGGATGGAACTACTTGCAAATGGGTTCGGGCAGGAAAATCGACCCAGAGCCTGGAAGGTGCAGAAGAACAGACGGCAAAAAATGGAGATGCTCCAAAGAGGCATACCCAGATTCTAAGTATTGTGAGAGACATATGCACAGAGGCAAAAACCGTTCAAGAAAGCCTGTGGAAGTTTTGAAACCAACAACAACAATAAGTAATGCTCTAAACTCTAATCCATCCACCCCAGCCATCTCCTCCATCACCCAGAACTCTACTCTCTCCTCTTCTTCTCATTCCCTTGCTTCCCTCAGCACCTCATTACCCTCTGAAACTCATACCCAACACCTCCACCATTCTTGTTTCAATACTCATCCTCCCCACCCTTTTCTCTACCAACATGCCTCTTCTTCTAGGCCCCCTGGTTCTGCTCATTTGCAGCAAAACTCCAACACCTCTTCCTTGCTTCTTGACCCAGGTTCTTACTCCCACAACAACCCAGATTACAGGTATGTTTATGGGGTGAAGGAAGATGTTGATGAGCATCCCTTTCTGTCGGAAGAACACTCTGGGAACATGAGAGGCTTCTCTGCTTCATCTATGGAAGATTCCTGGCAGCTGACTCCTCTGACAATGAGTTGCTCTTCCGCATCCTCCAGACACAAGAACTGCTCTGCTTTACAAGGTGACTACTCTTCCTACTTGCAGCTTCAGAGCTTTGGCGATTCCCCTAAGCAACTCAAGCAAGAGGAGCATTACTACGCGCTAGGAAAATATAACGAGATGCAGAGTAAGATGGAAAGAGAGGAACCACAGAAGACTATGCATCACTTCTTTGATGAATGGTCTCCAAAAGACAGAGAATCGTGGCTTGATTTGGATGATAAGCCATCCAATACTGGCTCAGTCTCTGCAACTCGGCTTTCAATAAGTTAA

Protein sequence

MSGGRNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKPTTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHLHHSCFNTHPPHPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLSEEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQLKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSATRLSIS
Homology
BLAST of Tan0000233 vs. ExPASy Swiss-Prot
Match: Q6AWY8 (Growth-regulating factor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF1 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.1e-48
Identity = 147/385 (38.18%), Postives = 197/385 (51.17%), Query Frame = 0

Query: 4   GRNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRT-SLDSPFI---SRLFPHQ 63
           G  R+PFTA+QWQELEHQALI+KYM SG P+P DL+  ++R+  LDS      S  FP Q
Sbjct: 13  GGGRYPFTASQWQELEHQALIYKYMASGTPIPSDLILPLRRSFLLDSALATSPSLAFPPQ 72

Query: 64  YPDVGWNYLQMGSGRKI-DPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKP 123
            P +GW    MG GRK  DPEPGRCRRTDGKKWRCSKEAYPDSKYCE+HMHRGKNRSRKP
Sbjct: 73  -PSLGWGCFGMGFGRKAEDPEPGRCRRTDGKKWRCSKEAYPDSKYCEKHMHRGKNRSRKP 132

Query: 124 VEVLKPTTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHLHHSCF 183
           VE+   T             P  SS T  ++ +S+  +  + +TS P+ ++++   H   
Sbjct: 133 VEMSLAT-----------PPPPSSSATSAASNTSAGVAPTTTTTSSPAPSYSRPAPHDA- 192

Query: 184 NTHPPHPFLY---QHASSSRPPGSAHLQQNSNTSSLLLD-------PGSYSHNNPDYRYV 243
               P+  LY     A+++R P +A      +   L LD       P  YS ++ +Y Y 
Sbjct: 193 ---APYQALYGGPYAAATARTPAAAAYHAQVSPFHLQLDTTHPHPPPSYYSMDHKEYAYG 252

Query: 244 YGVKEDVDEHPFLSE----EHSGNMRG-----FSASSMEDSWQLTPLTMSCSSASSRHKN 303
           +  KE   EH F S+    EH     G     F    ME     TPL      A   H  
Sbjct: 253 HATKEVHGEHAFFSDGTEREHHHAAAGHGQWQFKQLGMEPKQSTTPL---FPGAGYGHTA 312

Query: 304 CSALQGDYSSYLQLQSFGDSPKQLKQEEHYYALGKYNEMQSKMEREE---PQKTMHHFFD 360
            S    D S     +      +Q +Q++H + LG    ++     +     QK + HFFD
Sbjct: 313 ASPYAIDLSKEDDDEKERRQQQQQQQQQHCFLLGADLRLEKPAGHDHAAAAQKPLRHFFD 372

BLAST of Tan0000233 vs. ExPASy Swiss-Prot
Match: Q8L8A6 (Growth-regulating factor 5 OS=Arabidopsis thaliana OX=3702 GN=GRF5 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.6e-48
Identity = 158/411 (38.44%), Postives = 212/411 (51.58%), Query Frame = 0

Query: 2   SGGR--NRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQ 61
           S GR   R PFT  QW+ELEHQALI+KYMVSG+PVPP+L+F+I+R SLD+  +SRL PHQ
Sbjct: 7   SSGRTIGRPPFTPTQWEELEHQALIYKYMVSGVPVPPELIFSIRR-SLDTSLVSRLLPHQ 66

Query: 62  YPDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPV 121
              +GW   QMG GRK DPEPGRCRRTDGKKWRCS+EAYPDSKYCE+HMHRG+NR+RK +
Sbjct: 67  --SLGWGCYQMGFGRKPDPEPGRCRRTDGKKWRCSREAYPDSKYCEKHMHRGRNRARKSL 126

Query: 122 EVLKPTTT------ISNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHL 181
           +  + TTT      +S   N+NPS    SS + NS  SS+++S +S S    S ++   L
Sbjct: 127 DQNQTTTTPLTSPSLSFTNNNNPSPTLSSSSSSNS--SSTTYSASSSSMDAYSNSNRFGL 186

Query: 182 HHSCFNTH---PPHPFLYQHASSS--------RPPGSAHLQQNSNTSSLLLDPGSYSHNN 241
             S  NT      H   Y + S+S            +  L QN+N++S      S + ++
Sbjct: 187 GGSSSNTRGYFNSHSLDYPYPSTSPKQQQQTLHHASALSLHQNTNSTSQFNVLAS-ATDH 246

Query: 242 PDYRYVYGVKE---DVDEHPFLSEEHSGNMRGFSASSMEDSWQLTPLTMS---------- 301
            D+RY  G+ E    V E  F  E      R F  S      Q     M+          
Sbjct: 247 KDFRYFQGIGERVGGVGERTFFPEA----SRSFQDSPYHHHQQPLATVMNDPYHHCSTDH 306

Query: 302 ---------CSSASSRH---------KNCSALQGDYSSYLQLQSFGDSPKQLKQEEHYYA 359
                     SS+SS+H         + C  L  D  +        +S +Q + +E    
Sbjct: 307 NKIDHHHTYSSSSSSQHLHHDHDHRQQQCFVLGADMFNKPTRSVLANSSRQDQNQEE--- 366

BLAST of Tan0000233 vs. ExPASy Swiss-Prot
Match: A2XA73 (Growth-regulating factor 1 OS=Oryza sativa subsp. indica OX=39946 GN=GRF1 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.0e-47
Identity = 147/385 (38.18%), Postives = 197/385 (51.17%), Query Frame = 0

Query: 4   GRNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRT-SLDSPFI---SRLFPHQ 63
           G  R+PFTA+QWQELEHQALI+KYM SG P+P DL+  ++R+  LDS      S  FP Q
Sbjct: 13  GGGRYPFTASQWQELEHQALIYKYMASGTPIPSDLILPLRRSFLLDSALATSPSLAFPPQ 72

Query: 64  YPDVGWNYLQMGSGRKI-DPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKP 123
            P +GW    MG GRK  DPEPGRCRRTDGKKWRCSKEAYPDSKYCE+HMHRGKNRSRKP
Sbjct: 73  -PSLGWGCFGMGFGRKAEDPEPGRCRRTDGKKWRCSKEAYPDSKYCEKHMHRGKNRSRKP 132

Query: 124 VEVLKPTTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHLHHSCF 183
           VE+   T             P  SS T  ++ SS+  +  + +TS P+ ++++   H   
Sbjct: 133 VEMSLAT-----------PPPPSSSATSAASNSSAGVAPTTTTTSSPAPSYSRPAPHDA- 192

Query: 184 NTHPPHPFLY---QHASSSRPPGSAHLQQNSNTSSLLLD-------PGSYSHNNPDYRYV 243
               P+  LY     A+++R P +A      +   L +D       P  YS ++ +Y Y 
Sbjct: 193 ---APYQALYGGPYAAATARTPAAAAYHAQVSPFHLHIDTTHPHPPPSYYSMDHKEYAYG 252

Query: 244 YGVKEDVDEHPFLSE----EHSGNMRG-----FSASSMEDSWQLTPLTMSCSSASSRHKN 303
           +  KE   EH F S+    EH     G     F    ME     TPL      A   H  
Sbjct: 253 HATKEVHGEHAFFSDGTEREHHHAAAGHGQWQFKQLGMEPKQSTTPL---FPGAGYGHTA 312

Query: 304 CSALQGDYSSYLQLQSFGDSPKQLKQEEHYYALGKYNEMQSKMEREE---PQKTMHHFFD 360
            S    D S     +      +Q +Q++H + LG    ++     +     QK + HFFD
Sbjct: 313 ASPYAIDLSKEDDDEK-ERRQQQQQQQQHCFLLGADLRLEKPAGHDHAAAAQKPLRHFFD 372

BLAST of Tan0000233 vs. ExPASy Swiss-Prot
Match: Q6AWY6 (Growth-regulating factor 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF3 PE=3 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 1.6e-40
Identity = 142/365 (38.90%), Postives = 191/365 (52.33%), Query Frame = 0

Query: 9   PFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVGWNYL 68
           PFTAAQ++ELE QALI+KY+V+G+PVP DLL  I+R  LDS   SR + H  P +G+   
Sbjct: 53  PFTAAQYEELEQQALIYKYLVAGVPVPADLLLPIRR-GLDS-LASRFYHH--PVLGYGSY 112

Query: 69  QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKPTTTI 128
               G+K+DPEPGRCRRTDGKKWRCSKEA PDSKYCERHMHRG+NRSRKPVE        
Sbjct: 113 ---FGKKLDPEPGRCRRTDGKKWRCSKEAAPDSKYCERHMHRGRNRSRKPVE-------- 172

Query: 129 SNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHLHHSCFNTHPPHPFLY 188
           +  +  +   PA +     ++ +  +HS                L+ +  N    +    
Sbjct: 173 AQLVAPHSQPPATAPAAAVTSTAFQNHS----------------LYPAIANGGGANGGGG 232

Query: 189 QHASSSRPPGSAHLQQNSNTSSLLLDPGSYS-----HNNPDYRY-VYGVKEDVDEHPFLS 248
                   PGS  L   SNT   + +  SYS       N D+RY  YGV+        L+
Sbjct: 233 GGGGGGSAPGSFAL--GSNTQLHMDNAASYSTVAAGAGNKDFRYSAYGVRP-------LA 292

Query: 249 EEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQ 308
           +EHS  + G   +S+++SW L P   S  S SS       + G+ S  L   +    PK 
Sbjct: 293 DEHSPLITGAMDTSIDNSWCLLPSQTSTFSVSS-----YPMLGNLSE-LDQNTICSLPKV 352

Query: 309 LKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSAT 368
            ++   ++          K E +    T+  FFDEW PK R+SW DL D  ++  + SAT
Sbjct: 353 EREPLSFFGSDYVTVDSGKQENQ----TLRPFFDEW-PKARDSWPDLAD-DNSLATFSAT 365

BLAST of Tan0000233 vs. ExPASy Swiss-Prot
Match: Q6ZIK5 (Growth-regulating factor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF4 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.7e-40
Identity = 142/374 (37.97%), Postives = 186/374 (49.73%), Query Frame = 0

Query: 9   PFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVGWNYL 68
           PFTAAQ++ELE QALI+KY+V+G+PVPPDL+  I+R  LDS   +R + H  P +G+   
Sbjct: 64  PFTAAQYEELEQQALIYKYLVAGVPVPPDLVLPIRR-GLDS-LAARFYNH--PALGYGPY 123

Query: 69  QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKPTTTI 128
               G+K+DPEPGRCRRTDGKKWRCSKEA PDSKYCERHMHRG+NRSRKPVE      T 
Sbjct: 124 ---FGKKLDPEPGRCRRTDGKKWRCSKEAAPDSKYCERHMHRGRNRSRKPVE------TQ 183

Query: 129 SNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHLHHSCFNTHPPHPFLY 188
             A +  PS          S + S++  LA+ S     + H+                LY
Sbjct: 184 LVAQSQPPS----------SVVGSAAAPLAAASNGSSFQNHS----------------LY 243

Query: 189 QHASSSRPPGSAHLQQNSNTSSLLLDPGSYSH-------------NNPDYRY-VYGVKED 248
              + S   G      +S  S+L    GS  H                D RY  YG +  
Sbjct: 244 PAIAGSNGGGGGRNMPSSFGSAL----GSQLHMDNAAPYAAVGGGTGKDLRYTAYGTRSL 303

Query: 249 VDEHPFLSEEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQ 308
            DE   L  E          +S+E+ W+L P   S    SS           YS    L 
Sbjct: 304 ADEQSQLITEAIN-------TSIENPWRLLPSQNSPFPLSS-----------YSQLGALS 363

Query: 309 SFG-DSPKQLKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKP 368
             G ++P  L + +        N+  +    ++  +T+  FFDEW PK R+SW DL D+ 
Sbjct: 364 DLGQNTPSSLSKVQRQPLSFFGNDYAAVDSVKQENQTLRPFFDEW-PKGRDSWSDLADEN 375

BLAST of Tan0000233 vs. NCBI nr
Match: KAG7030511.1 (Growth-regulating factor 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 591.3 bits (1523), Expect = 5.8e-165
Identity = 304/365 (83.29%), Postives = 321/365 (87.95%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVG 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+TIKR+SLD+PFISRLFPHQYPDVG
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTIKRSSLDTPFISRLFPHQYPDVG 63

Query: 65  WNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 124
           WN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP
Sbjct: 64  WNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 123

Query: 125 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST-SLPSETHTQHLHHSCFNT-HP 184
           TTTI+ AL SNPSTP ISSIT NST        ASLST S PSE HT    HSCFNT  P
Sbjct: 124 TTTIT-ALTSNPSTPPISSITHNST--------ASLSTNSFPSEIHT----HSCFNTPPP 183

Query: 185 PHPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLS 244
           PHPFLYQH S SRPPGSAHLQ NSN S LL  P  Y   N   RYVYG KEDVD+HPF+S
Sbjct: 184 PHPFLYQHGSFSRPPGSAHLQHNSNPSLLLDPPADYRRRN---RYVYGAKEDVDKHPFVS 243

Query: 245 EEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQ 304
           EEHSGNMRGFSASSMED WQLTPLTMSCSS+S RHKNCSALQGDYSSYLQLQSFG SPKQ
Sbjct: 244 EEHSGNMRGFSASSMEDPWQLTPLTMSCSSSSPRHKNCSALQGDYSSYLQLQSFGGSPKQ 303

Query: 305 LKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSAT 364
           +KQ+EHYYA+GKYNEMQSKM+RE+P+KTMHHFF EWSPKDRESW+DLDDK SNTGSVS T
Sbjct: 304 VKQDEHYYAVGKYNEMQSKMDREKPEKTMHHFFGEWSPKDRESWVDLDDKSSNTGSVSET 352

Query: 365 RLSIS 368
           RLS+S
Sbjct: 364 RLSMS 352

BLAST of Tan0000233 vs. NCBI nr
Match: KAG6599533.1 (Growth-regulating factor 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 590.9 bits (1522), Expect = 7.6e-165
Identity = 304/365 (83.29%), Postives = 321/365 (87.95%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVG 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+TIKR+SLD+PFISRLFPHQYPDVG
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTIKRSSLDTPFISRLFPHQYPDVG 63

Query: 65  WNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 124
           WN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP
Sbjct: 64  WNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 123

Query: 125 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST-SLPSETHTQHLHHSCFNT-HP 184
           TTTI+ AL SNPSTP ISSIT NST        ASLST S PSE HT    HSCFNT  P
Sbjct: 124 TTTIT-ALTSNPSTPPISSITHNST--------ASLSTNSFPSEIHT----HSCFNTPPP 183

Query: 185 PHPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLS 244
           PHPFLYQH S SRPPGSAHLQ NSN S LL  P  Y  N    RYVYG KEDVD+HPF+S
Sbjct: 184 PHPFLYQHGSFSRPPGSAHLQHNSNPSLLLDPPADYRRN----RYVYGAKEDVDKHPFVS 243

Query: 245 EEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQ 304
           EEHSGNMRGFSASSMED WQLTPLTMSCSS+S RHKNCSALQGDYSSYLQLQSFG SPKQ
Sbjct: 244 EEHSGNMRGFSASSMEDPWQLTPLTMSCSSSSPRHKNCSALQGDYSSYLQLQSFGGSPKQ 303

Query: 305 LKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSAT 364
           +KQ+EHYYA+GKYNEMQSKM+RE+P+KTMHHFF EWSPKDRESW+DLDDK SNTGSVS T
Sbjct: 304 VKQDEHYYAVGKYNEMQSKMDREKPEKTMHHFFGEWSPKDRESWVDLDDKSSNTGSVSET 351

Query: 365 RLSIS 368
           RLS+S
Sbjct: 364 RLSMS 351

BLAST of Tan0000233 vs. NCBI nr
Match: XP_022999222.1 (growth-regulating factor 5-like [Cucurbita maxima])

HSP 1 Score: 590.5 bits (1521), Expect = 1.0e-164
Identity = 303/364 (83.24%), Postives = 320/364 (87.91%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVG 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+T+KR+SLD+PFISRLFPHQYPDVG
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTMKRSSLDTPFISRLFPHQYPDVG 63

Query: 65  WNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 124
           WN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP
Sbjct: 64  WNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 123

Query: 125 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST-SLPSETHTQHLHHSCFNTHPP 184
           TTTI+ AL SNPSTP ISSIT NST        ASLST S PSE HT    HSCFNT  P
Sbjct: 124 TTTIT-ALTSNPSTPPISSITHNST--------ASLSTNSFPSEIHT----HSCFNTPSP 183

Query: 185 HPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLSE 244
           HPFLYQH S SRPPGSAHLQ +SN S LL  P  Y  N    RYVYG KEDVD+HPFLSE
Sbjct: 184 HPFLYQHGSFSRPPGSAHLQHSSNPSLLLDPPADYRRN----RYVYGGKEDVDKHPFLSE 243

Query: 245 EHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQL 304
           EHSGNMRGFSASSMED WQLTPLTMSCSS+S RHKNCSALQGDYSSYLQLQSFG SPKQ+
Sbjct: 244 EHSGNMRGFSASSMEDPWQLTPLTMSCSSSSPRHKNCSALQGDYSSYLQLQSFGGSPKQV 303

Query: 305 KQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSATR 364
           KQ+EHYYA+GKYNEMQSKMERE+P+KTMHHFF EWSPKDRESW+DLDDK SNTGSVS TR
Sbjct: 304 KQDEHYYAVGKYNEMQSKMEREKPEKTMHHFFGEWSPKDRESWVDLDDKSSNTGSVSETR 350

Query: 365 LSIS 368
           LS+S
Sbjct: 364 LSMS 350

BLAST of Tan0000233 vs. NCBI nr
Match: XP_022946732.1 (growth-regulating factor 5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 586.3 bits (1510), Expect = 1.9e-163
Identity = 301/365 (82.47%), Postives = 319/365 (87.40%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVG 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+T+KR+SLD+PFISRLFPHQYPDVG
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTMKRSSLDTPFISRLFPHQYPDVG 63

Query: 65  WNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 124
           WN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP
Sbjct: 64  WNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 123

Query: 125 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST-SLPSETHTQHLHHSCFNT-HP 184
           T TI+ AL SNPSTP ISSIT NST        ASLST S PSE HT    HSCFNT  P
Sbjct: 124 TATIT-ALTSNPSTPPISSITHNST--------ASLSTNSFPSEIHT----HSCFNTPPP 183

Query: 185 PHPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLS 244
           PHPFLYQH S SRPPGSAH Q NSN S LL  P  Y  N    RYVYG KEDVD+HPF+S
Sbjct: 184 PHPFLYQHGSFSRPPGSAHFQHNSNPSLLLDPPADYRRN----RYVYGAKEDVDKHPFVS 243

Query: 245 EEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQ 304
           EEHSGNMRGFSASSMED WQLTPLTMSCSS+S RHKNCSALQGDYSSYLQLQSFG SPKQ
Sbjct: 244 EEHSGNMRGFSASSMEDPWQLTPLTMSCSSSSPRHKNCSALQGDYSSYLQLQSFGGSPKQ 303

Query: 305 LKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSAT 364
           +KQ+EHYYA+GKYNEMQSKM+RE+P+KTMHHFF EWSPKDRESW+DLDDK SNTGSVS T
Sbjct: 304 VKQDEHYYAVGKYNEMQSKMDREKPEKTMHHFFGEWSPKDRESWVDLDDKSSNTGSVSET 351

Query: 365 RLSIS 368
           RLS+S
Sbjct: 364 RLSMS 351

BLAST of Tan0000233 vs. NCBI nr
Match: XP_023546923.1 (growth-regulating factor 1-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.3 bits (1505), Expect = 7.2e-163
Identity = 301/365 (82.47%), Postives = 319/365 (87.40%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVG 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+ +KR+SLD+PFISRLFPHQYPDVG
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYAMKRSSLDTPFISRLFPHQYPDVG 63

Query: 65  WNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 124
           WN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP
Sbjct: 64  WNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 123

Query: 125 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST-SLPSETHTQHLHHSCFNT-HP 184
           TTTI+ AL SNPSTP ISSIT NST        ASLST S PSE HT    HSCFNT  P
Sbjct: 124 TTTIT-ALTSNPSTPPISSITHNST--------ASLSTNSFPSEIHT----HSCFNTPPP 183

Query: 185 PHPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLS 244
           PHPFLYQH S SRPPGSAHLQ NSN S LL  P  Y  N    RYVY  KEDVD+HPFLS
Sbjct: 184 PHPFLYQHGSFSRPPGSAHLQHNSNPSLLLDPPADYRRN----RYVYAGKEDVDKHPFLS 243

Query: 245 EEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQ 304
           EEHSGNMRGFSASSMED WQLTPLTMSCSS+S RHKNCSALQGDYSSYLQLQSFG SPKQ
Sbjct: 244 EEHSGNMRGFSASSMEDPWQLTPLTMSCSSSSPRHKNCSALQGDYSSYLQLQSFGGSPKQ 303

Query: 305 LKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSAT 364
           +KQ+EHYYA+GKYNEMQSKM+RE+P+KTMHHFF EWSPKDRESW+DLDDK SNTG+VS T
Sbjct: 304 VKQDEHYYAVGKYNEMQSKMDREKPEKTMHHFFGEWSPKDRESWVDLDDKSSNTGAVSET 351

Query: 365 RLSIS 368
           RLS+S
Sbjct: 364 RLSMS 351

BLAST of Tan0000233 vs. ExPASy TrEMBL
Match: A0A6J1KAB2 (Growth-regulating factor OS=Cucurbita maxima OX=3661 GN=LOC111493661 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.8e-165
Identity = 303/364 (83.24%), Postives = 320/364 (87.91%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVG 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+T+KR+SLD+PFISRLFPHQYPDVG
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTMKRSSLDTPFISRLFPHQYPDVG 63

Query: 65  WNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 124
           WN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP
Sbjct: 64  WNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 123

Query: 125 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST-SLPSETHTQHLHHSCFNTHPP 184
           TTTI+ AL SNPSTP ISSIT NST        ASLST S PSE HT    HSCFNT  P
Sbjct: 124 TTTIT-ALTSNPSTPPISSITHNST--------ASLSTNSFPSEIHT----HSCFNTPSP 183

Query: 185 HPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLSE 244
           HPFLYQH S SRPPGSAHLQ +SN S LL  P  Y  N    RYVYG KEDVD+HPFLSE
Sbjct: 184 HPFLYQHGSFSRPPGSAHLQHSSNPSLLLDPPADYRRN----RYVYGGKEDVDKHPFLSE 243

Query: 245 EHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQL 304
           EHSGNMRGFSASSMED WQLTPLTMSCSS+S RHKNCSALQGDYSSYLQLQSFG SPKQ+
Sbjct: 244 EHSGNMRGFSASSMEDPWQLTPLTMSCSSSSPRHKNCSALQGDYSSYLQLQSFGGSPKQV 303

Query: 305 KQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSATR 364
           KQ+EHYYA+GKYNEMQSKMERE+P+KTMHHFF EWSPKDRESW+DLDDK SNTGSVS TR
Sbjct: 304 KQDEHYYAVGKYNEMQSKMEREKPEKTMHHFFGEWSPKDRESWVDLDDKSSNTGSVSETR 350

Query: 365 LSIS 368
           LS+S
Sbjct: 364 LSMS 350

BLAST of Tan0000233 vs. ExPASy TrEMBL
Match: A0A6J1G4J3 (Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111450713 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 9.1e-164
Identity = 301/365 (82.47%), Postives = 319/365 (87.40%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVG 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+T+KR+SLD+PFISRLFPHQYPDVG
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTMKRSSLDTPFISRLFPHQYPDVG 63

Query: 65  WNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 124
           WN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP
Sbjct: 64  WNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKP 123

Query: 125 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST-SLPSETHTQHLHHSCFNT-HP 184
           T TI+ AL SNPSTP ISSIT NST        ASLST S PSE HT    HSCFNT  P
Sbjct: 124 TATIT-ALTSNPSTPPISSITHNST--------ASLSTNSFPSEIHT----HSCFNTPPP 183

Query: 185 PHPFLYQHASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKEDVDEHPFLS 244
           PHPFLYQH S SRPPGSAH Q NSN S LL  P  Y  N    RYVYG KEDVD+HPF+S
Sbjct: 184 PHPFLYQHGSFSRPPGSAHFQHNSNPSLLLDPPADYRRN----RYVYGAKEDVDKHPFVS 243

Query: 245 EEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQSFGDSPKQ 304
           EEHSGNMRGFSASSMED WQLTPLTMSCSS+S RHKNCSALQGDYSSYLQLQSFG SPKQ
Sbjct: 244 EEHSGNMRGFSASSMEDPWQLTPLTMSCSSSSPRHKNCSALQGDYSSYLQLQSFGGSPKQ 303

Query: 305 LKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPSNTGSVSAT 364
           +KQ+EHYYA+GKYNEMQSKM+RE+P+KTMHHFF EWSPKDRESW+DLDDK SNTGSVS T
Sbjct: 304 VKQDEHYYAVGKYNEMQSKMDREKPEKTMHHFFGEWSPKDRESWVDLDDKSSNTGSVSET 351

Query: 365 RLSIS 368
           RLS+S
Sbjct: 364 RLSMS 351

BLAST of Tan0000233 vs. ExPASy TrEMBL
Match: A0A6J1DMB1 (Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111021882 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.9e-163
Identity = 307/373 (82.31%), Postives = 320/373 (85.79%), Query Frame = 0

Query: 1   MSGGRNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQY 60
           MSG  +RFPFTAAQWQELEHQALIFKYMVSGIPVPPDLL+TIKR+SLDSPFISRLFP  Y
Sbjct: 1   MSGRSSRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTIKRSSLDSPFISRLFPQHY 60

Query: 61  PDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVE 120
           PDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVE
Sbjct: 61  PDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVE 120

Query: 121 VLKPTTTI-----SNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHLHH 180
           VLK TTT      + ALNSNPSTPAISSITQNS LS           SLPS+ H    +H
Sbjct: 121 VLKTTTTTTPTTPATALNSNPSTPAISSITQNSALS-----------SLPSDPHNH--NH 180

Query: 181 SCFNTHPPHPFLYQH-ASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKED 240
           SCFNTH PHPFLYQH +SSSRPPGSA L QNSNTS L         +NPDYRYVYG+KED
Sbjct: 181 SCFNTHSPHPFLYQHGSSSSRPPGSA-LHQNSNTSMLF--------DNPDYRYVYGLKED 240

Query: 241 VDEHPFLSEEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYLQLQ 300
            DEHPFLSEEHSGNMRGFSASS+EDSWQLTPLTMSCSS+SSRHKNCSALQGDYSSYLQLQ
Sbjct: 241 GDEHPFLSEEHSGNMRGFSASSLEDSWQLTPLTMSCSSSSSRHKNCSALQGDYSSYLQLQ 300

Query: 301 SFGDSPKQLKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDDKPS 360
           SFGDSPKQLKQEEHYY      EMQ+KM REEPQK MHHFFDEWSPKDRESWLDLDDK S
Sbjct: 301 SFGDSPKQLKQEEHYY------EMQNKMGREEPQKIMHHFFDEWSPKDRESWLDLDDKSS 345

Query: 361 NTGSVSATRLSIS 368
           NTGSVSATRLS+S
Sbjct: 361 NTGSVSATRLSMS 345

BLAST of Tan0000233 vs. ExPASy TrEMBL
Match: A0A6J1DPG3 (Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111021882 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.5e-161
Identity = 307/376 (81.65%), Postives = 320/376 (85.11%), Query Frame = 0

Query: 1   MSGGRNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQY 60
           MSG  +RFPFTAAQWQELEHQALIFKYMVSGIPVPPDLL+TIKR+SLDSPFISRLFP  Y
Sbjct: 1   MSGRSSRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTIKRSSLDSPFISRLFPQHY 60

Query: 61  PDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVE 120
           PDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVE
Sbjct: 61  PDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVE 120

Query: 121 VLKPTTTI-----SNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHLHH 180
           VLK TTT      + ALNSNPSTPAISSITQNS LS           SLPS+ H    +H
Sbjct: 121 VLKTTTTTTPTTPATALNSNPSTPAISSITQNSALS-----------SLPSDPHNH--NH 180

Query: 181 SCFNTHPPHPFLYQH-ASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDY---RYVYGV 240
           SCFNTH PHPFLYQH +SSSRPPGSA L QNSNTS L         +NPDY   RYVYG+
Sbjct: 181 SCFNTHSPHPFLYQHGSSSSRPPGSA-LHQNSNTSMLF--------DNPDYRRNRYVYGL 240

Query: 241 KEDVDEHPFLSEEHSGNMRGFSASSMEDSWQLTPLTMSCSSASSRHKNCSALQGDYSSYL 300
           KED DEHPFLSEEHSGNMRGFSASS+EDSWQLTPLTMSCSS+SSRHKNCSALQGDYSSYL
Sbjct: 241 KEDGDEHPFLSEEHSGNMRGFSASSLEDSWQLTPLTMSCSSSSSRHKNCSALQGDYSSYL 300

Query: 301 QLQSFGDSPKQLKQEEHYYALGKYNEMQSKMEREEPQKTMHHFFDEWSPKDRESWLDLDD 360
           QLQSFGDSPKQLKQEEHYY      EMQ+KM REEPQK MHHFFDEWSPKDRESWLDLDD
Sbjct: 301 QLQSFGDSPKQLKQEEHYY------EMQNKMGREEPQKIMHHFFDEWSPKDRESWLDLDD 348

Query: 361 KPSNTGSVSATRLSIS 368
           K SNTGSVSATRLS+S
Sbjct: 361 KSSNTGSVSATRLSMS 348

BLAST of Tan0000233 vs. ExPASy TrEMBL
Match: A0A6J1G4G2 (Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111450713 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.3e-159
Identity = 301/392 (76.79%), Postives = 319/392 (81.38%), Query Frame = 0

Query: 5   RNRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPD-- 64
           RNR PFTAAQWQELEHQALIFKYMVSGIPVPPDLL+T+KR+SLD+PFISRLFPHQYPD  
Sbjct: 4   RNRLPFTAAQWQELEHQALIFKYMVSGIPVPPDLLYTMKRSSLDTPFISRLFPHQYPDGN 63

Query: 65  -------------------------VGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEA 124
                                    VGWN++QMGSGRKIDPEPGRCRRTDGKKWRCSKEA
Sbjct: 64  TSYSSVLTRIIHYDAQVLGLYCVCAVGWNFVQMGSGRKIDPEPGRCRRTDGKKWRCSKEA 123

Query: 125 YPDSKYCERHMHRGKNRSRKPVEVLKPTTTISNALNSNPSTPAISSITQNSTLSSSSHSL 184
           YPDSKYCERHMHRGKNRSRKPVEVLKPT TI+ AL SNPSTP ISSIT NST        
Sbjct: 124 YPDSKYCERHMHRGKNRSRKPVEVLKPTATIT-ALTSNPSTPPISSITHNST-------- 183

Query: 185 ASLST-SLPSETHTQHLHHSCFNT-HPPHPFLYQHASSSRPPGSAHLQQNSNTSSLLLDP 244
           ASLST S PSE HT    HSCFNT  PPHPFLYQH S SRPPGSAH Q NSN S LL  P
Sbjct: 184 ASLSTNSFPSEIHT----HSCFNTPPPPHPFLYQHGSFSRPPGSAHFQHNSNPSLLLDPP 243

Query: 245 GSYSHNNPDYRYVYGVKEDVDEHPFLSEEHSGNMRGFSASSMEDSWQLTPLTMSCSSASS 304
             Y  N    RYVYG KEDVD+HPF+SEEHSGNMRGFSASSMED WQLTPLTMSCSS+S 
Sbjct: 244 ADYRRN----RYVYGAKEDVDKHPFVSEEHSGNMRGFSASSMEDPWQLTPLTMSCSSSSP 303

Query: 305 RHKNCSALQGDYSSYLQLQSFGDSPKQLKQEEHYYALGKYNEMQSKMEREEPQKTMHHFF 364
           RHKNCSALQGDYSSYLQLQSFG SPKQ+KQ+EHYYA+GKYNEMQSKM+RE+P+KTMHHFF
Sbjct: 304 RHKNCSALQGDYSSYLQLQSFGGSPKQVKQDEHYYAVGKYNEMQSKMDREKPEKTMHHFF 363

Query: 365 DEWSPKDRESWLDLDDKPSNTGSVSATRLSIS 368
            EWSPKDRESW+DLDDK SNTGSVS TRLS+S
Sbjct: 364 GEWSPKDRESWVDLDDKSSNTGSVSETRLSMS 378

BLAST of Tan0000233 vs. TAIR 10
Match: AT3G13960.1 (growth-regulating factor 5 )

HSP 1 Score: 193.7 bits (491), Expect = 2.5e-49
Identity = 158/411 (38.44%), Postives = 212/411 (51.58%), Query Frame = 0

Query: 2   SGGR--NRFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQ 61
           S GR   R PFT  QW+ELEHQALI+KYMVSG+PVPP+L+F+I+R SLD+  +SRL PHQ
Sbjct: 7   SSGRTIGRPPFTPTQWEELEHQALIYKYMVSGVPVPPELIFSIRR-SLDTSLVSRLLPHQ 66

Query: 62  YPDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPV 121
              +GW   QMG GRK DPEPGRCRRTDGKKWRCS+EAYPDSKYCE+HMHRG+NR+RK +
Sbjct: 67  --SLGWGCYQMGFGRKPDPEPGRCRRTDGKKWRCSREAYPDSKYCEKHMHRGRNRARKSL 126

Query: 122 EVLKPTTT------ISNALNSNPSTPAISSITQNSTLSSSSHSLASLSTSLPSETHTQHL 181
           +  + TTT      +S   N+NPS    SS + NS  SS+++S +S S    S ++   L
Sbjct: 127 DQNQTTTTPLTSPSLSFTNNNNPSPTLSSSSSSNS--SSTTYSASSSSMDAYSNSNRFGL 186

Query: 182 HHSCFNTH---PPHPFLYQHASSS--------RPPGSAHLQQNSNTSSLLLDPGSYSHNN 241
             S  NT      H   Y + S+S            +  L QN+N++S      S + ++
Sbjct: 187 GGSSSNTRGYFNSHSLDYPYPSTSPKQQQQTLHHASALSLHQNTNSTSQFNVLAS-ATDH 246

Query: 242 PDYRYVYGVKE---DVDEHPFLSEEHSGNMRGFSASSMEDSWQLTPLTMS---------- 301
            D+RY  G+ E    V E  F  E      R F  S      Q     M+          
Sbjct: 247 KDFRYFQGIGERVGGVGERTFFPEA----SRSFQDSPYHHHQQPLATVMNDPYHHCSTDH 306

Query: 302 ---------CSSASSRH---------KNCSALQGDYSSYLQLQSFGDSPKQLKQEEHYYA 359
                     SS+SS+H         + C  L  D  +        +S +Q + +E    
Sbjct: 307 NKIDHHHTYSSSSSSQHLHHDHDHRQQQCFVLGADMFNKPTRSVLANSSRQDQNQEE--- 366

BLAST of Tan0000233 vs. TAIR 10
Match: AT2G06200.1 (growth-regulating factor 6 )

HSP 1 Score: 164.9 bits (416), Expect = 1.3e-40
Identity = 80/120 (66.67%), Postives = 89/120 (74.17%), Query Frame = 0

Query: 7   RFPFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKR------TSLDSPFISRLFPHQY 66
           R PFT +QW+ELE+QAL+FKY+ + +PVPP LLF IKR      +S  S   S   P   
Sbjct: 4   RIPFTESQWEELENQALVFKYLAANMPVPPHLLFLIKRPFLFSSSSSSSSSSSFFSPTLS 63

Query: 67  PDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNR--SRKP 119
           P  GWN  +MG GRKID EPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNR  SRKP
Sbjct: 64  PHFGWNVYEMGMGRKIDAEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSSSRKP 123

BLAST of Tan0000233 vs. TAIR 10
Match: AT4G37740.1 (growth-regulating factor 2 )

HSP 1 Score: 134.0 bits (336), Expect = 2.4e-31
Identity = 112/304 (36.84%), Postives = 159/304 (52.30%), Query Frame = 0

Query: 9   PFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVGWNYL 68
           PFT  QW ELE QALI+KY+ + +PVP  LL +IK++    P+ S L P  +   GW   
Sbjct: 164 PFTLTQWAELEQQALIYKYITANVPVPSSLLISIKKSFY--PYGS-LPPSSF---GWGTF 223

Query: 69  QMG-SGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEV---LKP 128
            +G +G  +DPEPGRCRRTDGKKWRCS++A PD KYCERH++RG++RSRKPVEV      
Sbjct: 224 HLGFAGGNMDPEPGRCRRTDGKKWRCSRDAVPDQKYCERHINRGRHRSRKPVEVQSGQNQ 283

Query: 129 TTTISNALNSNPSTPAISSITQNSTLSSSSHSLASLST--------SLPSETHTQHLHHS 188
           T   ++   + P  P ++  T  S   +SS+   ++ +        SLP+      ++ S
Sbjct: 284 TAAAASKAVTTPQQPVVAGNTNRSNARASSNRSLAIGSQYINPSTESLPNNRGVS-IYPS 343

Query: 189 CFNTHPPH-PFLYQ-HASSSRPPGSAHLQQNSNTSSLLLDPGSYSHNNPDYRYVYGVKED 248
             N  P   P ++Q H +++ P    H+    ++ SLL         NP+    YG    
Sbjct: 344 TVNLQPKESPVIHQKHRNNNNPFEFGHI----SSDSLL---------NPNTAKTYG--SS 403

Query: 249 VDEHPFLSEEHSGNMRGFS-ASSMEDSWQLTPLTMS----CSSASSRHKNCSALQGDYSS 294
             +     E+HSGN    S    +   W  T L+MS     SS SS H N +A +    S
Sbjct: 404 FLDFSSNQEKHSGNHNHNSWPEELTSDW--TQLSMSIPIASSSPSSTHNNNNAQEKTTLS 443

BLAST of Tan0000233 vs. TAIR 10
Match: AT2G36400.1 (growth-regulating factor 3 )

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-29
Identity = 78/158 (49.37%), Postives = 101/158 (63.92%), Query Frame = 0

Query: 6   NRFP-----FTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSL---DSPFIS---R 65
           +RFP     F+ AQWQELE QALI++YM++G  VP +LL  IK++ L    S F+    +
Sbjct: 68  SRFPKMGSFFSWAQWQELELQALIYRYMLAGAAVPQELLLPIKKSLLHLSPSYFLHHPLQ 127

Query: 66  LFPHQYPDVGWNYLQMGSGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNR 125
             PH  P   W YL   +   +DPEPGRCRRTDGKKWRCS++ +   KYCERHMHRG+NR
Sbjct: 128 HLPHYQP--AW-YLGRAA---MDPEPGRCRRTDGKKWRCSRDVFAGHKYCERHMHRGRNR 187

Query: 126 SRKPVEVLKPTTTISNALNSNPSTPAISSITQNSTLSS 153
           SRKPVE   PTT  + A +   S  A ++ T  +T S+
Sbjct: 188 SRKPVET--PTTVNATATSMASSVAAAATTTTATTTST 217

BLAST of Tan0000233 vs. TAIR 10
Match: AT2G22840.1 (growth-regulating factor 1 )

HSP 1 Score: 125.6 bits (314), Expect = 8.5e-29
Identity = 73/170 (42.94%), Postives = 110/170 (64.71%), Query Frame = 0

Query: 9   PFTAAQWQELEHQALIFKYMVSGIPVPPDLLFTIKRTSLDSPFISRLFPHQYPDVGWNYL 68
           PF+  QW ELE QALI+KY+ + +PVP  LL ++K++    P+ S L P+ +   GW   
Sbjct: 133 PFSLTQWAELEQQALIYKYITANVPVPSSLLLSLKKSFF--PYGS-LPPNSF---GWGSF 192

Query: 69  QMG-SGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKPTTT 128
            +G SG  +DPEPGRCRRTDGKKWRCS++A PD KYCERH++RG++RSRKPVE      T
Sbjct: 193 HLGFSGGNMDPEPGRCRRTDGKKWRCSRDAVPDQKYCERHINRGRHRSRKPVEGQNGHNT 252

Query: 129 ISNALNSNPSTPAISSITQNSTLSSS-----SHSLASLSTSLPSETHTQH 173
            + A  S  +    +++++ +  +S+     S +  SL+ ++ ++ HT +
Sbjct: 253 NAAAAASAAAASTAAAVSKAAAGTSAVAMRGSDNNNSLAAAVGTQHHTNN 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6AWY82.1e-4838.18Growth-regulating factor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF1 PE=3... [more]
Q8L8A63.6e-4838.44Growth-regulating factor 5 OS=Arabidopsis thaliana OX=3702 GN=GRF5 PE=1 SV=1[more]
A2XA733.0e-4738.18Growth-regulating factor 1 OS=Oryza sativa subsp. indica OX=39946 GN=GRF1 PE=2 S... [more]
Q6AWY61.6e-4038.90Growth-regulating factor 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF3 PE=3... [more]
Q6ZIK52.7e-4037.97Growth-regulating factor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF4 PE=1... [more]
Match NameE-valueIdentityDescription
KAG7030511.15.8e-16583.29Growth-regulating factor 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6599533.17.6e-16583.29Growth-regulating factor 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022999222.11.0e-16483.24growth-regulating factor 5-like [Cucurbita maxima][more]
XP_022946732.11.9e-16382.47growth-regulating factor 5-like isoform X2 [Cucurbita moschata][more]
XP_023546923.17.2e-16382.47growth-regulating factor 1-like isoform X4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1KAB24.8e-16583.24Growth-regulating factor OS=Cucurbita maxima OX=3661 GN=LOC111493661 PE=3 SV=1[more]
A0A6J1G4J39.1e-16482.47Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111450713 PE=3 SV=1[more]
A0A6J1DMB15.9e-16382.31Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111021882 PE=3 SV=... [more]
A0A6J1DPG32.5e-16181.65Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111021882 PE=3 SV=... [more]
A0A6J1G4G22.3e-15976.79Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111450713 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13960.12.5e-4938.44growth-regulating factor 5 [more]
AT2G06200.11.3e-4066.67growth-regulating factor 6 [more]
AT4G37740.12.4e-3136.84growth-regulating factor 2 [more]
AT2G36400.11.3e-2949.37growth-regulating factor 3 [more]
AT2G22840.18.5e-2942.94growth-regulating factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014978Glutamine-Leucine-Glutamine, QLQSMARTSM00951QLQ_2coord: 8..44
e-value: 1.4E-12
score: 57.8
IPR014978Glutamine-Leucine-Glutamine, QLQPFAMPF08880QLQcoord: 9..42
e-value: 5.7E-16
score: 57.9
IPR014978Glutamine-Leucine-Glutamine, QLQPROSITEPS51666QLQcoord: 9..44
score: 22.766739
IPR014977WRC domainPFAMPF08879WRCcoord: 78..120
e-value: 7.5E-21
score: 73.5
IPR014977WRC domainPROSITEPS51667WRCcoord: 77..121
score: 24.979784
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 192..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 346..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 125..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..216
NoneNo IPR availablePANTHERPTHR31602:SF60GROWTH-REGULATING FACTOR 5coord: 3..353
IPR031137Growth-regulating factorPANTHERPTHR31602FAMILY NOT NAMEDcoord: 3..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000233.1Tan0000233.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0099402 plant organ development
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0032502 developmental process
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding