PI0007043 (gene) Melon (PI 482460) v1

Overview


Name	PI0007043
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	DNA-directed RNA polymerase II subunit 4-like
Location	chr09: 20608642 .. 20613161 (-)
RNA-Seq Expression	PI0007043
Synteny	PI0007043

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTATAGTATCATTCATTTAAGGTTGACTTATGTTGGACCTTTGGCCCCCAAATTTTTATCACAAGCTCGGCCTCATGTATGGCCAAAGCCGATCAGGCCCATGCTCTTCCTTGATCCATTATTTCGTTTTCTCTCGTTATCCTATTCTACCCTTGGTTATACAATCCACAACAACAACAAAAGCATAAAGCTTAAATACTATGAACTAAGATTCAATTGACTATCACATCAAAATTAATTAACATTAATTAATGTAATACAAAATTAATTAAATAGATATTAAATATATATTTTTAAAAAATATATTTTTGAATTGTTCTAAAAATATATCTTTTTAAAAGAATATAATATTTTTTTGGAGCATTGCATAAATTTATTCATAGAAATATTGGTAGATTTAATATAAATTGATTCTTTTAGGAAAAAAATGAAATTCTAATACCTTTGCCAACATTGATAATTTAATCATTGTATTAAATGTATTAAATACAAATTTATTAATGAAGGAAAATAAAAATGTTAAAATCACATTTTAATCTTTATCCTTTAACATAATTTAGAATGTTTTAGGTTAGTTTATATATTTTTAACAATAATAATATAATGAATTTTTGGGGTCTCTTCGTGGTTCATTAATAGGCAAAAAAAAAAAAAAAAAAAAAACTATGCAACTCTATTTCCATCATCTCAGAAAATATAGAAATTTATTATTGTTATTAAGATTATTTTTAAATACAGTAAAATAAACTAAAATATTAATACAATATTAACAAAATATCACTATCTTTTAATCAATAGATTAAGATATTTTTGCTATATTTATATATATATATATATATATATTTATTTATTTATTTATTTGAAGTTTCATCATTTAAAATAATTTTTATTTTATTTTCTTTTCTTCTTTTTTTTTTTTTTGTTTTTTTAAGACTTGGCTCTGTTTTGAAATTCCATAAACATTTTGGCTCCATTTCCCAAAACTTACACATATAAATGCCGCCGAAGAACGTGCAAATATTATGATCACCGTTATTGGCCTTTGTCCGAGTTGAAAACGAAGAAGCTGCCTCTGATTTTGCCGTCACGTTTCGCCGTCAGCCGGATCTCTGCGTTTTGTGGTGCGTCCTCTCCTCTCTCGGTTCTTTCTCTATTCTAATTTCAGAGGATGATTGAATCTTTAACACTAGAATTTGTCATTCATTTCCGTTCATCAAGATTACGAATCTCTTTTTTCTAATATAGGTATACGTTTTGCCATTCTCAGTTAAAGTTTCATTGCTGTTTAGACATTTAGTTAGGGCCTAATTGCCCCTCTCTGTTCTAAAAAACTGCTGATTATTATAGATCATACAAACTAGAATTTGATGCAGTTTTTGGAATTTAAATTAAAATGTTCTGATGTTCACAGCCTCGTAAATTGTATTTCTAAATTTAAACCATGTTTGATTAATTGGTAAACTTGATAGAAACCATGAAATACACGAGTGTATTCATGTGTTTTGTGAGTTACATCATGTGGACAATTTTCTAATGTTATAGACTAAATCAAGTAAAAGTGAATGAAGGAATTTCTTGTGTGATACCTTATTCAAATTCTTCTTTCTTAAGGTTATCAATTTTATGTATATGTAGTTGCTGGCTTTGAATTTCCCCTTCTTTTCCCATACTTCTGATGCCTTGCAATTTGGTTGTGTAGTTTGCTGTGTGGTTTGGTATTTTGATTAATTAGTGTAGCTTCACTAGCGGTTTTTGTAATATCATTCTTGTAGATTTAGTTTTTGATCTTGATCTACTCGTTTCCATATATTGTAGGTTGGACAAATCTTTAACTCGAAGCATGTCTGGAGAAGAGGAAGAAAATGCCGCAGAGCTTAAAATTGGGGATGGTACCATATTTTCCCATAGATCTCTAATTTTCTATCAATATTCAATTCATTGAGATTTTAAGTCACTAACATTCTATGGCAATGTGTTTGCATCGTATATTTGCAATTTTTTAATCTATTGAGCAATTCATGTGTTTGATCTTTTAATTTGCTTAAAAGTGTGAAACTTAATTCATTATGTTGTTCCCCATGCCTTAATGTACCCTTGTTGCACATTCTTTGAACTTTTTATTTGCCAATGTGGAGATTGTTTTTATGTTTTTAACAATGCATTTAGCTTGTAGTGGGAATGTGCAGTCAATGTAAACATTAATTACTCTTAAAGAAATTTTGTGCTTAGAGTGGTAACTTGAAGAAAAATCTTCTGTTTTTTTGCTAAGAAATCGAGATTTAATTGAGAAAAGTGAAAGTGTACCAGTGCATACAACCAAGCTCACAAGAGGAGTCGACGATTATAATGCAAAGAAGTTGCAATCTCTTTCATTAAATCACTACCTAGTAGATGGTTGTTTTGCTTAGTCGGGAGAGGAGAAATATAGTAAACTTCCTTGTTTCTTTAAGGCCATTAGTTTATGTGGTTACATCTTGTACCCCTCTATTTAGGATCGTAGAATCTCTTAACCGTTTCAGATATCGTTAGGTAAGAAATAGCCATTAGATAGGTAGTTGGACGTCCACGTTTATGAAAAGAAGTTTTGTTTGCATGCACCTTCCATTTTTGTTTATGCTATCATGCTATTATTTTCCGAATGCATGTTCTTAATATTTTTAGTTGGTTCACTTACTAATTTGAAACGTCACTTCCTTAATGATGTGAAAATTTGGCCAATTGGATCTAAAAAGCCCTAGTCCTTGTGACATGATTAAGACACCTCACTTCATTCTTGTTTAAGGTTGAAATAGGAAAATGTGAGTGTTTATCTCCGCAAAATCTTAATTCTAATCTATAATCGTTTTGTCAGTAAGACACTAAAACTATTGCTAGGTTTTCCAGGATAATGCTTAAGGCCTGAGACTAGCCAAATCTTTGTGATTTATATGGATAAGTAATTATTCAAAATTTAAGCATTTGCCATCAAAAATCTTTTTGGGGAGTACTCTTGCAGCCTTTTCATTTTCTTGTATTTATCTTGCTCTTTGTGCTCTTTGTGCTAGGGTCCATGAAGAGAAAATATAAGGCATCATAATTAACACTTTCTTTGTCTACTTGCAGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCCTTGATCCTGGAACACAAGTATGAGCAGCTTCAACAAAGGGCTGAGGATCCAACAAATCAAGTTTCTCAGTGAGATTTATGTTTATTCTTTATTGATTCATAAGTTAAGTTAGAGTTTCTCTTCTTTGTTGAAATAGCTAAAATGTATAGTTTTATTACGATATTCATTCTCACTTGCCATACAATAACAATGGATTTTTATTTTGGTCTTCTTTTGCAGAGTGTTTGAAAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAGTATCCTTTCTTATATCATTGATTCACCCAAAAGCTTAAGTTAGTAGGTAAAGGCAAATTTAATATAATATCTAACATAAACTATATACATTAATGTTTTTTATTTTATGGATGGTGGGAAGGATGAGCTGTGCATATCTACTCTATGCTATAACTAATCCCATTTTGAACTAGTAATTTACATTTGTCATTTTCTTGCTAGACCAATAACTCTCTGATTAGCTGTTATATATATATCTACATCCATACTATTTAGTGTTTTGTAGGTTTCCTAGGTATCCATTTGAAAAAACTGTCCTACTTTGGTATTTAATCACGGAGGGAAATACAATTTAAAGTTGGCTTCATTGTTTAAAATGGCATAAATATGGTTAAATCTTAACTGCTATCATATAGAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGGTATGTGTATAAGACCACTTATTTAATTAACTTATTGATTATTATTCTATGGAAAGGTTTATCCCATTGTTCTTGAAATTTGCAGTTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACTTCCATAGTTCCATCTTTGAAGGTATTTTTTTAGCCAACTTTGTATAATTATGCCTATATTAAATGGTAGTTTATTTTGTTGGTATTTTAATGTGCTGATCCTAAGTAGGTACTAACTTTTAAAAAGTTTATCGTTATGTCTTTTTTTATGCATCAAGTTTCTAAATTAGGCTAAAATGTCCATGTGTCAAGTTTTTAAAGTTTTTTAGAAACAAAACTTTAACGGTTTCCATCCAAAACTAATGATGAGTATTTTTTGAATTCTTGAGGTATTTTTGGAACTTTTGAAATTTCAAAGGCATGTTCGATACAAAGAGTAAAATTTAGGAACGTTTTCTATAATTTAGTCTTTAAATTGCTGCTAAAATTTTAAAGTGAAAACAAAATGTTTATAGATAGAGTAGATTTGCTTATAAATACTTTTATTGGTTTCAGACTAAAGGAAGAGTGCATGACGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATCAAAAAGTTTGAGTAA

mRNA sequence

ATGTGTTATAGTATCATTCATTTAAGGTTGACTTATGTTGGACCTTTGGCCCCCAAATTTTTATCACAAGCTCGGCCTCATGTATGGCCAAAGCCGATCAGGCCCATGCTCTTCCTTGATCCATTATTTCGTTTTCTCTCGTTATCCTATTCTACCCTTGTTGAAAACGAAGAAGCTGCCTCTGATTTTGCCGTCACGTTTCGCCGTCAGCCGGATCTCTGCGTTTTGTGGTTGGACAAATCTTTAACTCGAAGCATGTCTGGAGAAGAGGAAGAAAATGCCGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCCTTGATCCTGGAACACAAGTATGAGCAGCTTCAACAAAGGGCTGAGGATCCAACAAATCAAGTTTCTCAAGTGTTTGAAAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGTTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACTTCCATAGTTCCATCTTTGAAGACTAAAGGAAGAGTGCATGACGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATCAAAAAGTTTGAGTAA

Coding sequence (CDS)

ATGTGTTATAGTATCATTCATTTAAGGTTGACTTATGTTGGACCTTTGGCCCCCAAATTTTTATCACAAGCTCGGCCTCATGTATGGCCAAAGCCGATCAGGCCCATGCTCTTCCTTGATCCATTATTTCGTTTTCTCTCGTTATCCTATTCTACCCTTGTTGAAAACGAAGAAGCTGCCTCTGATTTTGCCGTCACGTTTCGCCGTCAGCCGGATCTCTGCGTTTTGTGGTTGGACAAATCTTTAACTCGAAGCATGTCTGGAGAAGAGGAAGAAAATGCCGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCCTTGATCCTGGAACACAAGTATGAGCAGCTTCAACAAAGGGCTGAGGATCCAACAAATCAAGTTTCTCAAGTGTTTGAAAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGTTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACTTCCATAGTTCCATCTTTGAAGACTAAAGGAAGAGTGCATGACGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATCAAAAAGTTTGAGTAA

Protein sequence

MCYSIIHLRLTYVGPLAPKFLSQARPHVWPKPIRPMLFLDPLFRFLSLSYSTLVENEEAASDFAVTFRRQPDLCVLWLDKSLTRSMSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAIEKMLNDLSLIKKFE

Homology

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BLAST of PI0007043 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.3e-66
Identity = 126/138 (91.30%), Postives = 133/138 (96.38%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA ++VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

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BLAST of PI0007043 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.0e-21
Identity = 57/137 (41.61%), Postives = 94/137 (68.61%), Query Frame = 0


Query: 87  SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 146
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 147 VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHDD 206
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 207 EAIEKMLNDLSLIKKFE 224
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

[Close]

BLAST of PI0007043 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 1.0e-21
Identity = 57/137 (41.61%), Postives = 94/137 (68.61%), Query Frame = 0


Query: 87  SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 146
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 147 VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHDD 206
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 207 EAIEKMLNDLSLIKKFE 224
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

[Close]

BLAST of PI0007043 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.8e-19
Identity = 55/147 (37.41%), Postives = 87/147 (59.18%), Query Frame = 0


Query: 89  EEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAED-------------PTNQ 148
           +EEE+   LK   +   AK L+N EV+++LEH+    +    +               N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 149 VSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVP 208
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA S++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 209 SLKTKGRVHDDEAIEKMLNDLSLIKKF 223
           SLK   ++ DDE ++ +L++LS ++KF
Sbjct: 132 SLK---KMEDDE-LQAILDELSNLRKF 154

[Close]

BLAST of PI0007043 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 88.6 bits (218), Expect = 9.8e-17
Identity = 47/126 (37.30%), Postives = 80/126 (63.49%), Query Frame = 0


Query: 91  EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRF 150
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  + D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 151 SRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAIE 210
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA +++PSL  +GR  D+E + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGRFEDEE-LR 130

Query: 211 KMLNDL 217
           ++L+D+
Sbjct: 131 QILDDI 131

[Close]

BLAST of PI0007043 vs. ExPASy TrEMBL
Match: A0A1S3CA78 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC103498588 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.3e-69
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. ExPASy TrEMBL
Match: A0A5A7T811 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001820 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.3e-69
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 6.3e-67
Identity = 133/138 (96.38%), Postives = 135/138 (97.83%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. ExPASy TrEMBL
Match: A0A6J1FFS7 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445330 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 6.3e-67
Identity = 133/138 (96.38%), Postives = 135/138 (97.83%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. ExPASy TrEMBL
Match: A0A5D3C019 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002310 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.4e-66
Identity = 131/138 (94.93%), Postives = 136/138 (98.55%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEA ++VPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]

BLAST of PI0007043 vs. NCBI nr
Match: XP_008459458.1 (PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >XP_008459459.1 PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >KAA0039380.1 DNA-directed RNA polymerase II subunit 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 271.9 bits (694), Expect = 4.8e-69
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. NCBI nr
Match: XP_004141497.1 (DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_031742047.1 DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >KAE8648963.1 hypothetical protein Csa_008563 [Cucumis sativus])

HSP 1 Score: 270.8 bits (691), Expect = 1.1e-68
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. NCBI nr
Match: XP_038890773.1 (DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida] >XP_038890774.1 DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida])

HSP 1 Score: 269.6 bits (688), Expect = 2.4e-68
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. NCBI nr
Match: XP_022939401.1 (DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022993195.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_022993196.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_023549816.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >XP_023549818.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >KAG6578503.1 DNA-directed RNA polymerase II subunit 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.8 bits (673), Expect = 1.3e-66
Identity = 133/138 (96.38%), Postives = 135/138 (97.83%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of PI0007043 vs. NCBI nr
Match: XP_038884885.1 (DNA-directed RNA polymerase II subunit 4 [Benincasa hispida])

HSP 1 Score: 263.1 bits (671), Expect = 2.2e-66
Identity = 132/138 (95.65%), Postives = 136/138 (98.55%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA ++VPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]

BLAST of PI0007043 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.6e-67
Identity = 126/138 (91.30%), Postives = 133/138 (96.38%), Query Frame = 0


Query: 86  MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 145
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 146 YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 205
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA ++VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 206 DEAIEKMLNDLSLIKKFE 224
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]

BLAST of PI0007043 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-16
Identity = 51/144 (35.42%), Postives = 85/144 (59.03%), Query Frame = 0


Query: 80  KSLTRSMSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQV 139
           KS   + S +   ++ ELK   E   A+C+M+CE   IL+    QL   +EDP+ ++   
Sbjct: 65  KSDKPTKSSKNSLHSFELKDLPE--NAECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVS 124

Query: 140 FEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKT 199
           ++++L YV+    Y NP +VR+V E L  Y +++ E+CV+ N   E+V+E  + +PSLKT
Sbjct: 125 YDRALAYVESCVHYTNPQSVRKVLEPLKTYGISDGEMCVIANASSESVDEVLAFIPSLKT 184

Query: 200 KGRVHDDEAIEKMLNDLSLIKKFE 224
           K  V  ++ ++  L +LS +KK E
Sbjct: 185 KKEV-INQPLQDALEELSKLKKSE 205

The following BLAST results are available for this feature:

BLAST of PI0007043 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48890	2.3e-66	91.30	DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]
O15514	1.0e-21	41.61	DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE...	[more]
Q9D7M8	1.0e-21	41.61	DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P...	[more]
Q54S04	2.8e-19	37.41	DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689...	[more]
Q9VEA5	9.8e-17	37.30	DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G...	[more]

BLAST of PI0007043 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CA78	2.3e-69	99.28	DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5A7T811	2.3e-69	99.28	DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1JVN3	6.3e-67	96.38	DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1FFS7	6.3e-67	96.38	DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A5D3C019	1.4e-66	94.93	DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of PI0007043 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459458.1	4.8e-69	99.28	PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >XP_0084...	[more]
XP_004141497.1	1.1e-68	99.28	DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_031742047.1 DNA-d...	[more]
XP_038890773.1	2.4e-68	98.55	DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida] >XP_038890774....	[more]
XP_022939401.1	1.3e-66	96.38	DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402...	[more]
XP_038884885.1	2.2e-66	95.65	DNA-directed RNA polymerase II subunit 4 [Benincasa hispida]	[more]

BLAST of PI0007043 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09920.1	1.6e-67	91.30	RNA polymerase II, Rpb4, core protein	[more]
AT4G15950.1	2.9e-16	35.42	RNA polymerase II, Rpb4, core protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006590	RNA polymerase Rpb4/RPC9, core	SMART	SM00657	rpol4neu2	coord: 101..223 e-value: 5.3E-57 score: 205.4
IPR005574	RNA polymerase subunit RPB4/RPC9	PFAM	PF03874	RNA_pol_Rpb4	coord: 108..217 e-value: 2.1E-23 score: 83.0
IPR038324	Rpb4/RPC9 superfamily	GENE3D	1.20.1250.40		coord: 86..223 e-value: 3.8E-50 score: 171.1
None	No IPR available	PANTHER	PTHR21297	DNA-DIRECTED RNA POLYMERASE II	coord: 86..223
None	No IPR available	PANTHER	PTHR21297:SF3	DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKE	coord: 86..223
IPR010997	HRDC-like superfamily	SUPERFAMILY	47819	HRDC-like	coord: 86..221

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0007043.1	PI0007043.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031990	mRNA export from nucleus in response to heat stress
biological_process	GO:0000288	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process	GO:0045948	positive regulation of translational initiation
biological_process	GO:0034402	recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0000932	P-body
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0030880	RNA polymerase complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0031369	translation initiation factor binding