PI0023483 (gene) Melon (PI 482460) v1

Overview


Name	PI0023483
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	nifU-like protein 1, chloroplastic
Location	chr01: 25483564 .. 25485921 (+)
RNA-Seq Expression	PI0023483
Synteny	PI0023483

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAATATTTGAAAGTTTAAATTAAAATTGTAATACTTATTTAAATTTAAAATATACTTTAATTGCAATGTCTCTAAAATTGTCAAATACCAATAAATACCAATTTTACACAAAAGCCCTCCAGGAGGGTGCGGTGCTGCAGAACAACTACAACCTCATCATCGCCTAGATTCCTCTTCGTTTCTTCTTCATTAATGGCGTCCTTAACCACCTCCGGCCTCCTCAAACCCCTCTTAAGAATTCCCAATCTCCTGCAAATTATCTCCAATATCCACGTTTTGCTTATGGGCAACATCAACTACCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGAGCTCCTCACCAGGGCTGTACTCCGCTCTGAAATTTGAACTGACCATCGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGTGGGTAGATTTCTTGTCTCGTTGAATCGAATGGGTTTGTTTTAATTTCCTTGTTAATTTAATTTTGTGTGTTGAAATTTTTGTTACTTAACAATTGGTTGCTGTTGGGGCATGTGCGGGGACTTTTTTTCTTTGGTGTTTCTATACTTTAGCAAATTGTGTTGAATATCTGTTACACATTATGGTATTTAGTAGTATCTGCACTTTAGAGATTTGAGTCTCATTCATTTCTTTCATCGAAGCAATCCTGGGATCGTATGTATGGCTTAGGACAAAGTTGACATCTTTTTGATTCTCGGTCTGAATATGCCCCTTAGCCCACTCTCTTTTCACATAGCCAAATAGTTGGTGTACGCTTTCTCGACGCTGGCCCGATAGAAGTGATTCCTTTTTCTCAAAAAGAATGGTCTCGATCTTCTTCTTTCTTAGCATTACTGTAAACCATTTGATCCTAGTCGTCTTGTGTGGAAGGAGCAATATGACAGAGATTTTGCTTTTCTCACTCCTGTTCGACCACCCGATTGATGAAGACTCGGAACTAACCACCCCGCTTTTGGAGACAACGAAGACTTTACTCGATGTCTTGATCCATCCATACTCTGCTAATCTAATCCCAACGTCTTTAATTCTGACGAGCTTGTCTCGGAGAGATCTCTATCTTTTGTTTGTTACATAACACTGAAGGCTCCACTAGAAAAGGAAGGAAAAAGGAAAATACCTCCAATGAGAAATCCCAAGTTCAAGATTAGTGCTGCAGTTCTATTTACAATCTTCAACTTTCCTGAGTACAATCTTCCTCCATTAGCACTGAATCTATTGGAAATTTGAAACAGTGACTTGGGCAGATGGATATGATTTTCCTTTCCTTATATGAGGGTCTTTCCAATAAAAAGGATTTGATGATTTGATATCTATCTTTAAGGAAGACCACGAAAAGTATCCTTAATCTGTGAACACTTTTAACCAATCTTGTGGGAGATTTGGTATATACCTAATAGGAATGTTTCTCAGGACATGGGTGGGGTTTTCATGACATTTGAAGGGACTGCAGACTGATGTAGCCTTTCGGTTTCTTTCCTGCATATACTAGTTGGAGTACATTTGATTTATTATCTTGGTCTAGGTCAGGTTTGGACAGTGCCATCATCTTGTACTTGTTACTGGTGAGTTTTATCTGTAATATCAAGTAGGGTGAAAAAGATCTCCTTGAACTAAATGGCAAAAGGAAGTCTTGTAACTCTCACTTCACTTTCATCGAATAAGGGTGTGAGTAAGAACTTTGTTGATTTGATGTTTAACTGCTTTGCATTCCCATGAAGTCATGAACAAAAAGTAACAATGATAAACATTTTTAACTAAAAATGTTTGAATCGATCTTCAGGAGCATGCGGTAGCTGCCCTAGCTCAACAACCACCATGAAGATGGGGATCGAACGTGTACTAAAGGAAAAGTTTGGAGATTCGGTAAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAGACTACAGCTGAGGTGAGTTTTCTATAGACGAGCAAATACAAAATTGGCTGATGTCAAAATTCAAACAAAAACAGAATGAGCTGAATAAAATAGGTAATTAGTATTAATCCTGGATGTTGCACTAATGCCAGGCAGTAAACAGTCACCTCGACATATTGAGGCCCGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCGATCAACGGTGGCGATTGCCTGGTCAAATATGAAGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

mRNA sequence

ATGAATTCCCAATCTCCTGCAAATTATCTCCAATATCCACGTTTTGCTTATGGGCAACATCAACTACCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGAGCTCCTCACCAGGGCTGTACTCCGCTCTGAAATTTGAACTGACCATCGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACCACCATGAAGATGGGGATCGAACGTGTACTAAAGGAAAAGTTTGGAGATTCGGTAAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAGACTACAGCTGAGGCAGTAAACAGTCACCTCGACATATTGAGGCCCGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCGATCAACGGTGGCGATTGCCTGGTCAAATATGAAGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

Coding sequence (CDS)

ATGAATTCCCAATCTCCTGCAAATTATCTCCAATATCCACGTTTTGCTTATGGGCAACATCAACTACCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGAGCTCCTCACCAGGGCTGTACTCCGCTCTGAAATTTGAACTGACCATCGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACCACCATGAAGATGGGGATCGAACGTGTACTAAAGGAAAAGTTTGGAGATTCGGTAAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAGACTACAGCTGAGGCAGTAAACAGTCACCTCGACATATTGAGGCCCGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCGATCAACGGTGGCGATTGCCTGGTCAAATATGAAGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

Protein sequence

MNSQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS

Homology

[Close]

BLAST of PI0023483 vs. ExPASy Swiss-Prot
Match: Q93W77 (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 9.9e-64
Identity = 125/171 (73.10%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 36  RASPSNSGPSTSSSPGLYSALKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSL 95
           + SP  SG S+    GLYSA  F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSL
Sbjct: 65  KISPLASGVSS----GLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSL 124

Query: 96  KLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTAEAVNSHLDILRP 155
           KL GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRP
Sbjct: 125 KLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRP 184

Query: 156 AIRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 207
           AI+NYGGSVEV+S+ G DC+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 185 AIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

[Close]

BLAST of PI0023483 vs. ExPASy Swiss-Prot
Match: Q93W20 (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 5.1e-20
Identity = 58/152 (38.16%), Postives = 86/152 (56.58%), Query Frame = 0


Query: 56  LKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGI 115
           L+  LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGI
Sbjct: 81  LEVPLTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGI 140

Query: 116 ERVLKEKFGDSVKEICQVYDEEPKETTAEAVNSHLDILRP-AIRNYGGSVEVISINGGDC 175
           ER L EK  + V       +E   E   E +   L+ +RP  I    GS++++ I     
Sbjct: 141 ERRLMEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIV 200

Query: 176 LVKYEGPE----SIGTGVKAAIKERFPDITNV 203
            ++  GP     ++   V   ++E+ P I  V
Sbjct: 201 KIRITGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of PI0023483 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.7e-20
Identity = 70/179 (39.11%), Postives = 99/179 (55.31%), Query Frame = 0


Query: 31  PGRAIRASPSNSGPS--TSSSPGL--YSALKFELTIGNVDLVLEDVRPYLIADGGNVDVV 90
           P   + A+P++  P     +  GL   +A++  LT GNV+ VL+ VRPYL ADGG+V + 
Sbjct: 42  PATRLVAAPNHQRPPLVVGAIAGLDPVTAVQLPLTAGNVESVLDQVRPYLTADGGDVALH 101

Query: 91  SVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEP-KETTAEA 150
            +   VV LKL GACGSCPSS  T+K GIER L EK  D V  +  V D+E   E   E 
Sbjct: 102 EIAGNVVRLKLQGACGSCPSSLITIKRGIERRLMEKIPD-VAAVEPVTDKETGLELNEEN 161

Query: 151 VNSHLDILRPAIRNY-GGSVEVISINGGDCLVKYEGPESIGTGVKAAI----KERFPDI 200
           V   L+ +RP +    GG ++ + I G    V+  GP ++   V+ A+    +E+ P I
Sbjct: 162 VEKVLNEIRPYLAGTGGGGLQFLMIKGPIVKVRLTGPAAVVRTVRIAVSKKLREKIPSI 219

[Close]

BLAST of PI0023483 vs. ExPASy Swiss-Prot
Match: Q84RQ7 (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.3e-17
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 60  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 119
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 120 KEKFGDSVKEICQVYDEEP--KETTAEAVNSHLDILRPAIRNY-GGSVEVISINGGDCLV 179
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 180 KYEGPESIGTGVKAAIKER 196
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of PI0023483 vs. ExPASy Swiss-Prot
Match: O32119 (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.1e-13
Identity = 37/70 (52.86%), Postives = 53/70 (75.71%), Query Frame = 0


Query: 65  VDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFG 124
           V  VL+ +RP+L+ DGG+ ++V V++G+V L+L+GACGSCPSST T+K GIER L E+  
Sbjct: 43  VQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEEV- 102

Query: 125 DSVKEICQVY 135
             V E+ QV+
Sbjct: 103 PGVVEVEQVF 111

[Close]

BLAST of PI0023483 vs. ExPASy TrEMBL
Match: A0A5A7TT28 (NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001900 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 7.5e-107
Identity = 199/205 (97.07%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 2   NSQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELT 61
           NSQSPANY QYP FAYGQHQLPVSLKRAFPGRA+RASPSNSGPSTSSSPGLYSA KFELT
Sbjct: 16  NSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPSTSSSPGLYSAQKFELT 75

Query: 62  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 121
           IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE
Sbjct: 76  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 135

Query: 122 KFGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 181
           KFGDSVKEICQVYDEEPKETT EAVN+HLDILRPAIRNYGGSVEVISINGGDCLVKYEGP
Sbjct: 136 KFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 195

Query: 182 ESIGTGVKAAIKERFPDITNVVFSS 207
           ESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 196 ESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of PI0023483 vs. ExPASy TrEMBL
Match: A0A0A0KB98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G412870 PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.2e-106
Identity = 200/205 (97.56%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 2   NSQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELT 61
           NSQSPAN LQYP FAYGQHQLPVSLKRAFPGRAIRASPSNSGPST+SSPGLYSA KFELT
Sbjct: 16  NSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTTSSPGLYSAQKFELT 75

Query: 62  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 121
           IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE
Sbjct: 76  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 135

Query: 122 KFGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 181
           KFGDSVKEICQVYDEEPKETT EAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP
Sbjct: 136 KFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 195

Query: 182 ESIGTGVKAAIKERFPDITNVVFSS 207
           ESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 196 ESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of PI0023483 vs. ExPASy TrEMBL
Match: A0A1S3BQX1 (nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.9e-106
Identity = 198/205 (96.59%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 2   NSQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELT 61
           NSQSPANY QYP FAYGQHQLPVSLKRAFPGRA+RASPSNSGPSTSSSPGLYSA KFELT
Sbjct: 16  NSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPSTSSSPGLYSAQKFELT 75

Query: 62  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 121
           IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE
Sbjct: 76  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 135

Query: 122 KFGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 181
           KFGDSVKEICQVYDEEPKETT EAVN+HLDILRPAIRNYGGSVEVISINGG+CLVKYEGP
Sbjct: 136 KFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEVISINGGNCLVKYEGP 195

Query: 182 ESIGTGVKAAIKERFPDITNVVFSS 207
           ESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 196 ESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of PI0023483 vs. ExPASy TrEMBL
Match: A0A6J1CRB9 (nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014036 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.0e-96
Identity = 184/203 (90.64%), Postives = 189/203 (93.10%), Query Frame = 0


Query: 3   SQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELTI 62
           SQ PANY QYP  A+ QHQ P SLKR  P R I+AS SNSGPSTSSSPGLYSA KFELTI
Sbjct: 17  SQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPSTSSSPGLYSAQKFELTI 76

Query: 63  GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK 122
           GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK
Sbjct: 77  GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK 136

Query: 123 FGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPE 182
           FGDSVKEICQVYDEEPKETT EAVNSHLD+LRPAIRNYGGSVEVISI+GGDCLVKYEGPE
Sbjct: 137 FGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEVISIDGGDCLVKYEGPE 196

Query: 183 SIGTGVKAAIKERFPDITNVVFS 206
           SIGTG+KAAIKERFPDITNVVFS
Sbjct: 197 SIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of PI0023483 vs. ExPASy TrEMBL
Match: A0A6J1JN01 (nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-94
Identity = 179/204 (87.75%), Postives = 191/204 (93.63%), Query Frame = 0


Query: 3   SQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELTI 62
           SQ+ AN+ QYP  A+GQHQ+PVSL RA P ++IRAS SNSGPSTSSSPGLYSA KFELTI
Sbjct: 17  SQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPSTSSSPGLYSAQKFELTI 76

Query: 63  GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK 122
            NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPSSTTTMKMGIERVLKEK
Sbjct: 77  ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEK 136

Query: 123 FGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPE 182
           FGDSVKEICQVYDEEPKE T EAVNSHLDILRPAIRNYGGSVEVISI+GG+C+V YEGPE
Sbjct: 137 FGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVMYEGPE 196

Query: 183 SIGTGVKAAIKERFPDITNVVFSS 207
           SIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 197 SIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of PI0023483 vs. NCBI nr
Match: KAA0046713.1 (nifU-like protein 1 [Cucumis melo var. makuwa] >TYK18249.1 nifU-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 396.4 bits (1017), Expect = 1.6e-106
Identity = 199/205 (97.07%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 2   NSQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELT 61
           NSQSPANY QYP FAYGQHQLPVSLKRAFPGRA+RASPSNSGPSTSSSPGLYSA KFELT
Sbjct: 16  NSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPSTSSSPGLYSAQKFELT 75

Query: 62  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 121
           IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE
Sbjct: 76  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 135

Query: 122 KFGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 181
           KFGDSVKEICQVYDEEPKETT EAVN+HLDILRPAIRNYGGSVEVISINGGDCLVKYEGP
Sbjct: 136 KFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 195

Query: 182 ESIGTGVKAAIKERFPDITNVVFSS 207
           ESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 196 ESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of PI0023483 vs. NCBI nr
Match: XP_004135981.1 (nifU-like protein 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 394.8 bits (1013), Expect = 4.5e-106
Identity = 200/205 (97.56%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 2   NSQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELT 61
           NSQSPAN LQYP FAYGQHQLPVSLKRAFPGRAIRASPSNSGPST+SSPGLYSA KFELT
Sbjct: 16  NSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTTSSPGLYSAQKFELT 75

Query: 62  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 121
           IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE
Sbjct: 76  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 135

Query: 122 KFGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 181
           KFGDSVKEICQVYDEEPKETT EAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP
Sbjct: 136 KFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 195

Query: 182 ESIGTGVKAAIKERFPDITNVVFSS 207
           ESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 196 ESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of PI0023483 vs. NCBI nr
Match: XP_008451442.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 394.4 bits (1012), Expect = 5.9e-106
Identity = 198/205 (96.59%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 2   NSQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELT 61
           NSQSPANY QYP FAYGQHQLPVSLKRAFPGRA+RASPSNSGPSTSSSPGLYSA KFELT
Sbjct: 16  NSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPSTSSSPGLYSAQKFELT 75

Query: 62  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 121
           IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE
Sbjct: 76  IGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKE 135

Query: 122 KFGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGP 181
           KFGDSVKEICQVYDEEPKETT EAVN+HLDILRPAIRNYGGSVEVISINGG+CLVKYEGP
Sbjct: 136 KFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEVISINGGNCLVKYEGP 195

Query: 182 ESIGTGVKAAIKERFPDITNVVFSS 207
           ESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 196 ESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of PI0023483 vs. NCBI nr
Match: XP_038898528.1 (nifU-like protein 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 367.1 bits (941), Expect = 1.0e-97
Identity = 187/203 (92.12%), Postives = 192/203 (94.58%), Query Frame = 0


Query: 3   SQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELTI 62
           SQ+PANY QY  FA+GQHQLP+SLKRA P RAIRAS SNS PSTSSSPGLYSA KFELTI
Sbjct: 17  SQTPANYRQYTPFAHGQHQLPISLKRASPRRAIRASASNSSPSTSSSPGLYSAQKFELTI 76

Query: 63  GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK 122
            NVDLVLEDVRPYLIADGGNVDVVS+EDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK
Sbjct: 77  RNVDLVLEDVRPYLIADGGNVDVVSIEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK 136

Query: 123 FGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPE 182
           FGDSVKEICQVYDE PKETT EAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPE
Sbjct: 137 FGDSVKEICQVYDEVPKETTPEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPE 196

Query: 183 SIGTGVKAAIKERFPDITNVVFS 206
           SIGTGVKAAIKERFPDIT+VVFS
Sbjct: 197 SIGTGVKAAIKERFPDITHVVFS 219

[Close]

BLAST of PI0023483 vs. NCBI nr
Match: XP_022144330.1 (nifU-like protein 1, chloroplastic [Momordica charantia])

HSP 1 Score: 361.7 bits (927), Expect = 4.2e-96
Identity = 184/203 (90.64%), Postives = 189/203 (93.10%), Query Frame = 0


Query: 3   SQSPANYLQYPRFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTSSSPGLYSALKFELTI 62
           SQ PANY QYP  A+ QHQ P SLKR  P R I+AS SNSGPSTSSSPGLYSA KFELTI
Sbjct: 17  SQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPSTSSSPGLYSAQKFELTI 76

Query: 63  GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK 122
           GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK
Sbjct: 77  GNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEK 136

Query: 123 FGDSVKEICQVYDEEPKETTAEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPE 182
           FGDSVKEICQVYDEEPKETT EAVNSHLD+LRPAIRNYGGSVEVISI+GGDCLVKYEGPE
Sbjct: 137 FGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEVISIDGGDCLVKYEGPE 196

Query: 183 SIGTGVKAAIKERFPDITNVVFS 206
           SIGTG+KAAIKERFPDITNVVFS
Sbjct: 197 SIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of PI0023483 vs. TAIR 10
Match: AT4G01940.1 (NFU domain protein 1 )

HSP 1 Score: 244.6 bits (623), Expect = 7.0e-65
Identity = 125/171 (73.10%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 36  RASPSNSGPSTSSSPGLYSALKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSL 95
           + SP  SG S+    GLYSA  F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSL
Sbjct: 65  KISPLASGVSS----GLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSL 124

Query: 96  KLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTAEAVNSHLDILRP 155
           KL GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRP
Sbjct: 125 KLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRP 184

Query: 156 AIRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 207
           AI+NYGGSVEV+S+ G DC+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 185 AIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

[Close]

BLAST of PI0023483 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 99.4 bits (246), Expect = 3.6e-21
Identity = 58/152 (38.16%), Postives = 86/152 (56.58%), Query Frame = 0


Query: 56  LKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGI 115
           L+  LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGI
Sbjct: 81  LEVPLTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGI 140

Query: 116 ERVLKEKFGDSVKEICQVYDEEPKETTAEAVNSHLDILRP-AIRNYGGSVEVISINGGDC 175
           ER L EK  + V       +E   E   E +   L+ +RP  I    GS++++ I     
Sbjct: 141 ERRLMEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIV 200

Query: 176 LVKYEGPE----SIGTGVKAAIKERFPDITNV 203
            ++  GP     ++   V   ++E+ P I  V
Sbjct: 201 KIRITGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of PI0023483 vs. TAIR 10
Match: AT4G25910.1 (NFU domain protein 3 )

HSP 1 Score: 89.4 bits (220), Expect = 3.8e-18
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 60  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 119
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 120 KEKFGDSVKEICQVYDEEP--KETTAEAVNSHLDILRPAIRNY-GGSVEVISINGGDCLV 179
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 180 KYEGPESIGTGVKAAIKER 196
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of PI0023483 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 83.6 bits (205), Expect = 2.1e-16
Identity = 46/96 (47.92%), Postives = 63/96 (65.62%), Query Frame = 0


Query: 56  LKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGI 115
           L+  LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGI
Sbjct: 81  LEVPLTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGI 140

Query: 116 ERVLKEKFGDSVKEICQVYDEEPKETTAEAVNSHLD 152
           ER L EK    + EI  V     +ET  E    +++
Sbjct: 141 ERRLMEK----IPEIVAVEALPDEETGLELNEENIE 172

[Close]

BLAST of PI0023483 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 54.7 bits (130), Expect = 1.0e-07
Identity = 32/85 (37.65%), Postives = 50/85 (58.82%), Query Frame = 0


Query: 61  TIGNVDLVLED-VRPYLIADGGNVDVVSV--EDGVVSLKLVGACGSCPSSTTTMKMGIER 120
           T+  +  +LE  +RP +  DGG+++      E G+V L++ GAC  CPSS+ T+K GIE 
Sbjct: 192 TVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIEN 251

Query: 121 VLKEKFGDSVKEICQVYDEEPKETT 143
           +L   +   VK + Q +D E +E T
Sbjct: 252 MLMH-YVSEVKGVEQEFDGEDEEGT 275

The following BLAST results are available for this feature:

BLAST of PI0023483 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93W77	9.9e-64	73.10	NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1...	[more]
Q93W20	5.1e-20	38.16	NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1...	[more]
Q84LK7	6.7e-20	39.11	NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N...	[more]
Q84RQ7	5.3e-17	40.29	NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2...	[more]
O32119	6.1e-13	52.86	Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224...	[more]

BLAST of PI0023483 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TT28	7.5e-107	97.07	NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G...	[more]
A0A0A0KB98	2.2e-106	97.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G412870 PE=3 SV=1	[more]
A0A1S3BQX1	2.9e-106	96.59	nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 ...	[more]
A0A6J1CRB9	2.0e-96	90.64	nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101403...	[more]
A0A6J1JN01	1.1e-94	87.75	nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 P...	[more]

BLAST of PI0023483 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0046713.1	1.6e-106	97.07	nifU-like protein 1 [Cucumis melo var. makuwa] >TYK18249.1 nifU-like protein 1 [...	[more]
XP_004135981.1	4.5e-106	97.56	nifU-like protein 1, chloroplastic [Cucumis sativus]	[more]
XP_008451442.1	5.9e-106	96.59	PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo]	[more]
XP_038898528.1	1.0e-97	92.12	nifU-like protein 1, chloroplastic [Benincasa hispida]	[more]
XP_022144330.1	4.2e-96	90.64	nifU-like protein 1, chloroplastic [Momordica charantia]	[more]

BLAST of PI0023483 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01940.1	7.0e-65	73.10	NFU domain protein 1	[more]
AT5G49940.1	3.6e-21	38.16	NIFU-like protein 2	[more]
AT4G25910.1	3.8e-18	40.29	NFU domain protein 3	[more]
AT5G49940.2	2.1e-16	47.92	NIFU-like protein 2	[more]
AT3G20970.1	1.0e-07	37.65	NFU domain protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 142..204 e-value: 4.0E-10 score: 41.7
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 57..141 e-value: 7.0E-27 score: 95.3
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 62..135
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 140..203
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 66..125 e-value: 8.4E-23 score: 80.3 coord: 148..202 e-value: 2.2E-4 score: 21.3
None	No IPR available	PANTHER	PTHR11178:SF15	NIFU-LIKE PROTEIN 1, CHLOROPLASTIC	coord: 39..205
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 39..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0023483.1	PI0023483.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding