MS023689 (gene) Bitter gourd (TR) v1

Overview


Name	MS023689
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pre-mRNA cleavage factor Im 25 kDa subunit
Location	scaffold570: 255660 .. 257487 (+)
RNA-Seq Expression	MS023689
Synteny	MS023689

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGACAACGGCCACTTGCATGCTATGGAGGACGATGAAGCTGCCACCGCCGAACAAAGCCGGGCCGTACGTATTTACCCTCTCGGCAACTACTACTTTGGATCCAAAGAGCCTCTTCTTTTCAAGAACGAGACCTTGGCCGATCGCCTCCACAGGATGAAATCCAAGTATCCACTCTCACTTTCCTCCCTTGTTTTCGGTTTACCTTCGTTTTGTTTCTCTCATGTGTTTTGTTTTATTGCAGCTATGCTGCTAACGGATTGAGGACTTGCGTCGAAGCAGTTTTGCTGGTCTGTTCTTTATTCTTTTTCGTTTTTCCTTCGCATATATATATTTTTTCTTGTACGTTCTTTAATAGACGACTTCCATTTTCGCTATTTCACGGCTTTGAGTGCTTGGTTTCTTTTAAGCATGTACAAGTTTTACTGTTGCTCTGCTATTACGATGATTTAATTACTTGACACCTACTTAATCGGTGGAGACCTAATATTGTAGGTTCGGTTTCGTCCAGTTATACTGCGATTAATATGTCGTCGGGCTAGTCAGATCATGATACCTGATTACGGAGAATGAAACTATACATCTTTCAGTTAATTTAGGATTCTGAAGTTTTGCCACTTTCCTGTATTAGATTTAGTGAGATTGGTTCCTTATGTTGCGAATGGACATGATTTTTGCATCTATAGTTGATCTCATTACTTAATTCCATTTTGAATCCACCCCAAAGAGAAGCGTGGCGGACTGGACTCTATGATTCGTAAATTTTTTGGGGAGAAATCTCTTTGAATGATGATTGAATCTCAATTCAGGAAGCTTTTCTGCTATAAAATTTTTAAATGGACATTTCATCTTGCGATAAACACACTGCTAACTGCATTGTAATTGAAGCGTCCTTTGTTCTTAAATTAGGTTGAACTATTCAAGCATCCTCACTTGTTGCTATTGCAAATACGAAATTCAATTTTCAAGCTCCCTGGCGGTCGCCTAAGGCCCAATGAATCAGGTAAATATGTGCAAGCAGCTGCATTTGTCTAATAGTTGTACTAACGGATATCTTACCGTGGTGGACGATCTAATTTTACAGATATTGATGGCCTGAAGCGAAAGTTGTCAAGGAAGCTTTCTGCCAATGGAACCTCTGATGCATCTGATTGGGAGGTATTACAGTGCTTCAATCTATTTCTTTCTAGTTTTCTACCATGTATGTACTTCTGATTTTGAATGATGGAATTCGAAATTTTCTTCTTTAATGTTTAGGTTGGTGAGTGTCTTGGTATGTGGTGGAGGCCTGACTTTGAAACCTTGCTCTATCCATATTTGTCCAGTAATGTAAAAGGGGCCAAGGTAGTTACTTAGATACAAGCTACTTCCTTTAAACTATGATTATGAGGCGATACATCATTACCAGTAACTCACTTGCTACCTGATGCAGGAATGCACTAAACTTTTCCTAGTCAAGTTGCCAGAGAGTCGAAAATTCATCGTCCCAAAGAACCTCAAGTTGATCGCAGTTCCTTTATGCCAACTTCACGAAAATCAGAAGGTATCCTAATCAAGACACTTTGTGGAAGTATAATTGGTGATATATATTTGAAGTTCTATCTTGCTTTCCTTTAAGGATCAAAAGAAAATACAGTTCAATTTCGTTGAGTCGTGTCAAAACATGGTGCATAATCCAACGACCTGTGAACTTATAGTCTTATCCACCATCTTTGACACAACTTTTCCTTCGCAAATTAATCCTGTCATCTTGCAGACATATGGACCAGTTATATCGGGTGTTCCGCAGCTACTTTCTAAGTTCTCCTTCAATATCATTGAAGCT

mRNA sequence

ATGGGAGACAACGGCCACTTGCATGCTATGGAGGACGATGAAGCTGCCACCGCCGAACAAAGCCGGGCCGTACGTATTTACCCTCTCGGCAACTACTACTTTGGATCCAAAGAGCCTCTTCTTTTCAAGAACGAGACCTTGGCCGATCGCCTCCACAGGATGAAATCCAACTATGCTGCTAACGGATTGAGGACTTGCGTCGAAGCAGTTTTGCTGGTTGAACTATTCAAGCATCCTCACTTGTTGCTATTGCAAATACGAAATTCAATTTTCAAGCTCCCTGGCGGTCGCCTAAGGCCCAATGAATCAGATATTGATGGCCTGAAGCGAAAGTTGTCAAGGAAGCTTTCTGCCAATGGAACCTCTGATGCATCTGATTGGGAGGTTGGTGAGTGTCTTGGTATGTGGTGGAGGCCTGACTTTGAAACCTTGCTCTATCCATATTTGTCCAGTAATGTAAAAGGGGCCAAGGAATGCACTAAACTTTTCCTAGTCAAGTTGCCAGAGAGTCGAAAATTCATCGTCCCAAAGAACCTCAAGTTGATCGCAGTTCCTTTATGCCAACTTCACGAAAATCAGAAGACATATGGACCAGTTATATCGGGTGTTCCGCAGCTACTTTCTAAGTTCTCCTTCAATATCATTGAAGCT

Coding sequence (CDS)

ATGGGAGACAACGGCCACTTGCATGCTATGGAGGACGATGAAGCTGCCACCGCCGAACAAAGCCGGGCCGTACGTATTTACCCTCTCGGCAACTACTACTTTGGATCCAAAGAGCCTCTTCTTTTCAAGAACGAGACCTTGGCCGATCGCCTCCACAGGATGAAATCCAACTATGCTGCTAACGGATTGAGGACTTGCGTCGAAGCAGTTTTGCTGGTTGAACTATTCAAGCATCCTCACTTGTTGCTATTGCAAATACGAAATTCAATTTTCAAGCTCCCTGGCGGTCGCCTAAGGCCCAATGAATCAGATATTGATGGCCTGAAGCGAAAGTTGTCAAGGAAGCTTTCTGCCAATGGAACCTCTGATGCATCTGATTGGGAGGTTGGTGAGTGTCTTGGTATGTGGTGGAGGCCTGACTTTGAAACCTTGCTCTATCCATATTTGTCCAGTAATGTAAAAGGGGCCAAGGAATGCACTAAACTTTTCCTAGTCAAGTTGCCAGAGAGTCGAAAATTCATCGTCCCAAAGAACCTCAAGTTGATCGCAGTTCCTTTATGCCAACTTCACGAAAATCAGAAGACATATGGACCAGTTATATCGGGTGTTCCGCAGCTACTTTCTAAGTTCTCCTTCAATATCATTGAAGCT

Protein sequence

MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAANGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANGTSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIEA

Homology

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BLAST of MS023689 vs. NCBI nr
Match: XP_022154764.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Momordica charantia])

HSP 1 Score: 446.4 bits (1147), Expect = 1.4e-121
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA
Sbjct: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
           NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
           TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIEA 218
           LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIEA
Sbjct: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIEA 217

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BLAST of MS023689 vs. NCBI nr
Match: KAG6586493.1 (Pre-mRNA cleavage factor Im 25 kDa subunit 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021351.1 Pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.4 bits (1012), Expect = 6.2e-106
Identity = 189/216 (87.50%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGD+G +HAMEDDE+A A+Q RA+ IYPL NYYFGSKEPL+FK++TLADR+ RMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVVRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
            GLRT VEAVLLVELFKHPH+LLLQIRNSIF LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
             DASDWEVG+CLG WWRPDFETLLYPYLS+NVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           LIAVPLCQLHEN K YGPVISG+PQLLSKFSFNIIE
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 216

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BLAST of MS023689 vs. NCBI nr
Match: XP_023536755.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.0 bits (1011), Expect = 8.1e-106
Identity = 189/216 (87.50%), Postives = 203/216 (93.98%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGD+G +HAMEDDE+ATA+Q RA+ IYPL NYYFGSKEPL+FK++TLADR+ RMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESATADQIRAIDIYPLSNYYFGSKEPLVFKDKTLADRVIRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
            GLRT VEAVLLVELFKHPH+LLLQIRNSIF LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
             DASDWEVG+CLG WWRPDFETLLYP+LS+NVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           LIAVPLCQLHEN K YGPVISG+PQLLSKFSFNIIE
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 216

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BLAST of MS023689 vs. NCBI nr
Match: XP_022937740.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita moschata])

HSP 1 Score: 391.3 bits (1004), Expect = 5.3e-105
Identity = 187/216 (86.57%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGD+G +HAMEDDE+A A+Q RA+ IYPL NYYFGSKEPL+FK++TLADR+ RMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
            GLRT VEAVLLVELFKHPH+LLLQIRNSIF LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
             DASDWEVG+CLG WWRPDFETLLYP+LS+NVKGAKECTKLFLVKLPE+RKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPENRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           LIAVPLCQLHEN K YGPVISG+PQLLSKFSFNIIE
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 216

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BLAST of MS023689 vs. NCBI nr
Match: XP_022965443.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita maxima])

HSP 1 Score: 391.3 bits (1004), Expect = 5.3e-105
Identity = 188/216 (87.04%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGD+G +HAMEDDE+A A+Q RA+ IYPL NYYFGSKEPL+FK++TLADR+ RMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
            GLRT VEAVLLVELFKHPH+LLLQIRNSIF LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
           + DASDWEVG+CLG WWRPDFETLL PYLS+NVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           LIAVPLCQLHEN K YGPVISG+PQLLSKFSFNIIE
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 216

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BLAST of MS023689 vs. ExPASy Swiss-Prot
Match: Q94AF0 (Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=CFIS1 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 1.4e-81
Identity = 144/221 (65.16%), Postives = 177/221 (80.09%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVR-----IYPLGNYYFGSKEPLLFKNETLADRLHRMK 60
           MG+      ME+    T  ++  V      +YPL +YYFGSKE L  K+E ++DR+ R+K
Sbjct: 1   MGEEARALDMEEISDNTTRRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRLK 60

Query: 61  SNYAANGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRK 120
           SNYAA+GLRTCVEAVLLVELFKHPH+LLLQ RNSIFKLPGGRLRP ESDI+GLKRKL+ K
Sbjct: 61  SNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLASK 120

Query: 121 LSANGTSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIV 180
           LS N     S +EVGEC+GMWWRP+FETL+YP+L  N+K  KECTKLFLV+LP  ++F+V
Sbjct: 121 LSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFVV 180

Query: 181 PKNLKLIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           PKN KL+AVPLCQLHEN+KTYGP++S +P+LLSKFSFN++E
Sbjct: 181 PKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMME 221

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BLAST of MS023689 vs. ExPASy Swiss-Prot
Match: Q8GXS3 (Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=CFIS2 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.6e-62
Identity = 110/197 (55.84%), Postives = 143/197 (72.59%), Query Frame = 0


Query: 21  SRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAANGLRTCVEAVLLVELFKHPH 80
           S+ V  YPL NY FG+KEP L K+ ++ADRL RMK NY   G+RT VE +LLV+   HPH
Sbjct: 4   SQVVNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPH 63

Query: 81  LLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANGTSDASDWEVGECLGMWWRPD 140
           +LLLQI N+  KLPGGRL+P E++ DGLKRKL+ KL  N  +   DW VGEC+  WWRP+
Sbjct: 64  ILLLQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPN 123

Query: 141 FETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENQKTYGPVI 200
           FET++YPY   ++   KEC +L++V L E   F VPKNLKL+AVPL +L++N + YGPVI
Sbjct: 124 FETMMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVI 183

Query: 201 SGVPQLLSKFSFNIIEA 218
           S +PQ LS+F FN+I +
Sbjct: 184 STIPQQLSRFHFNMISS 200

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BLAST of MS023689 vs. ExPASy Swiss-Prot
Match: Q7T3C6 (Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955 GN=nudt21 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.2e-48
Identity = 92/193 (47.67%), Postives = 128/193 (66.32%), Query Frame = 0


Query: 22  RAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAANGLRTCVEAVLLVELFKHPHL 81
           R + +YPL NY FG+KEPL  K+ ++A R  RM+  +   G+R  VE VL+V   + PH+
Sbjct: 36  RTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFEKIGMRRTVEGVLIVHEHRLPHV 95

Query: 82  LLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANGTSDA--SDWEVGECLGMWWRP 141
           LLLQ+  + FKLPGG L P E +++GLKR ++  L   G  D    DW + +C+G WWRP
Sbjct: 96  LLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEIL---GRQDGVKQDWVIDDCIGNWWRP 155

Query: 142 DFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENQKTYGPV 201
           +FE   YPY+ +++   KE  KLFLV+L E   F VPKN KL+A PL +L++N   YGP+
Sbjct: 156 NFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPI 215

Query: 202 ISGVPQLLSKFSF 213
           IS +PQLLS+F+F
Sbjct: 216 ISSLPQLLSRFNF 225

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BLAST of MS023689 vs. ExPASy Swiss-Prot
Match: Q3ZCA2 (Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 GN=NUDT21 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.6e-48
Identity = 92/193 (47.67%), Postives = 128/193 (66.32%), Query Frame = 0


Query: 22  RAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAANGLRTCVEAVLLVELFKHPHL 81
           R + +YPL NY FG+KEPL  K+ ++A R  RM+  +   G+R  VE VL+V   + PH+
Sbjct: 35  RTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHV 94

Query: 82  LLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANGTSDA--SDWEVGECLGMWWRP 141
           LLLQ+  + FKLPGG L P E +++GLKR ++  L   G  D    DW + +C+G WWRP
Sbjct: 95  LLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEIL---GRQDGVLQDWVIDDCIGNWWRP 154

Query: 142 DFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENQKTYGPV 201
           +FE   YPY+ +++   KE  KLFLV+L E   F VPKN KL+A PL +L++N   YGP+
Sbjct: 155 NFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPI 214

Query: 202 ISGVPQLLSKFSF 213
           IS +PQLLS+F+F
Sbjct: 215 ISSLPQLLSRFNF 224

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BLAST of MS023689 vs. ExPASy Swiss-Prot
Match: O43809 (Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=9606 GN=NUDT21 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.6e-48
Identity = 92/193 (47.67%), Postives = 128/193 (66.32%), Query Frame = 0


Query: 22  RAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAANGLRTCVEAVLLVELFKHPHL 81
           R + +YPL NY FG+KEPL  K+ ++A R  RM+  +   G+R  VE VL+V   + PH+
Sbjct: 35  RTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHV 94

Query: 82  LLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANGTSDA--SDWEVGECLGMWWRP 141
           LLLQ+  + FKLPGG L P E +++GLKR ++  L   G  D    DW + +C+G WWRP
Sbjct: 95  LLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEIL---GRQDGVLQDWVIDDCIGNWWRP 154

Query: 142 DFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENQKTYGPV 201
           +FE   YPY+ +++   KE  KLFLV+L E   F VPKN KL+A PL +L++N   YGP+
Sbjct: 155 NFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPI 214

Query: 202 ISGVPQLLSKFSF 213
           IS +PQLLS+F+F
Sbjct: 215 ISSLPQLLSRFNF 224

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BLAST of MS023689 vs. ExPASy TrEMBL
Match: A0A6J1DN47 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111021937 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 6.7e-122
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA
Sbjct: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
           NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
           TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIEA 218
           LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIEA
Sbjct: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIEA 217

[Close]

BLAST of MS023689 vs. ExPASy TrEMBL
Match: A0A6J1HNY2 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita maxima OX=3661 GN=LOC111465348 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.5e-105
Identity = 188/216 (87.04%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGD+G +HAMEDDE+A A+Q RA+ IYPL NYYFGSKEPL+FK++TLADR+ RMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
            GLRT VEAVLLVELFKHPH+LLLQIRNSIF LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
           + DASDWEVG+CLG WWRPDFETLL PYLS+NVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           LIAVPLCQLHEN K YGPVISG+PQLLSKFSFNIIE
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 216

[Close]

BLAST of MS023689 vs. ExPASy TrEMBL
Match: A0A6J1FHN4 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC111444047 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.5e-105
Identity = 187/216 (86.57%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGD+G +HAMEDDE+A A+Q RA+ IYPL NYYFGSKEPL+FK++TLADR+ RMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
            GLRT VEAVLLVELFKHPH+LLLQIRNSIF LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
             DASDWEVG+CLG WWRPDFETLLYP+LS+NVKGAKECTKLFLVKLPE+RKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPENRKFIVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           LIAVPLCQLHEN K YGPVISG+PQLLSKFSFNIIE
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 216

[Close]

BLAST of MS023689 vs. ExPASy TrEMBL
Match: A0A0A0LIM0 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G000310 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.8e-103
Identity = 180/215 (83.72%), Postives = 201/215 (93.49%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60
           MGD+G LHA++DD+A++A Q   + IYPL NYYFGSKEPLLFK+ETL+DR+ RMKSNYAA
Sbjct: 1   MGDDGRLHAIDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRVLRMKSNYAA 60

Query: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120
           +GLRTCVEAV+LVELFKHPHLLL QIRNSIFKLPGGR+RPNESDIDGL RKL++KLSANG
Sbjct: 61  HGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRIRPNESDIDGLTRKLTKKLSANG 120

Query: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180
            SDAS WEV ECLGMWWRPDFETLL+PYLS+NVKGAKECTKLFLVKLPESRKF+VPKNLK
Sbjct: 121 ASDASHWEVSECLGMWWRPDFETLLFPYLSTNVKGAKECTKLFLVKLPESRKFVVPKNLK 180

Query: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNII 216
           LIAVPLCQ+HEN KTYGP+ISG+PQLLSKFSFNII
Sbjct: 181 LIAVPLCQIHENHKTYGPIISGIPQLLSKFSFNII 215

[Close]

BLAST of MS023689 vs. ExPASy TrEMBL
Match: A0A1S3C9Z9 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103498651 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.0e-101
Identity = 181/217 (83.41%), Postives = 199/217 (91.71%), Query Frame = 0


Query: 1   MGDNGHLHAM--EDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNY 60
           MGD+G LHA+  +DD+A++A Q   + IYPL NYYFGSKEPLLFK+ETL+DR+ RMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60

Query: 61  AANGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSA 120
           AA GLRTCVEAV+LVELFKHPHLLL QIRNSIFKLPGGRLRPNESDIDGL RKLS+KL A
Sbjct: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120

Query: 121 NGTSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKN 180
           NG SDASDWEV ECLGMWWRPDFETLL+PYLS+NVKGAKEC KLFLVKLPES+KF+VPKN
Sbjct: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 181 LKLIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNII 216
           LKLIAVPLCQ+HEN KTYGPVISG+PQLLSKFSFNII
Sbjct: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNII 217

[Close]

BLAST of MS023689 vs. TAIR 10
Match: AT4G29820.1 (homolog of CFIM-25 )

HSP 1 Score: 303.9 bits (777), Expect = 1.0e-82
Identity = 144/221 (65.16%), Postives = 177/221 (80.09%), Query Frame = 0


Query: 1   MGDNGHLHAMEDDEAATAEQSRAVR-----IYPLGNYYFGSKEPLLFKNETLADRLHRMK 60
           MG+      ME+    T  ++  V      +YPL +YYFGSKE L  K+E ++DR+ R+K
Sbjct: 1   MGEEARALDMEEISDNTTRRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRLK 60

Query: 61  SNYAANGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRK 120
           SNYAA+GLRTCVEAVLLVELFKHPH+LLLQ RNSIFKLPGGRLRP ESDI+GLKRKL+ K
Sbjct: 61  SNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLASK 120

Query: 121 LSANGTSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIV 180
           LS N     S +EVGEC+GMWWRP+FETL+YP+L  N+K  KECTKLFLV+LP  ++F+V
Sbjct: 121 LSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFVV 180

Query: 181 PKNLKLIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 217
           PKN KL+AVPLCQLHEN+KTYGP++S +P+LLSKFSFN++E
Sbjct: 181 PKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMME 221

[Close]

BLAST of MS023689 vs. TAIR 10
Match: AT4G25550.1 (Cleavage/polyadenylation specificity factor, 25kDa subunit )

HSP 1 Score: 240.0 bits (611), Expect = 1.8e-63
Identity = 110/197 (55.84%), Postives = 143/197 (72.59%), Query Frame = 0


Query: 21  SRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAANGLRTCVEAVLLVELFKHPH 80
           S+ V  YPL NY FG+KEP L K+ ++ADRL RMK NY   G+RT VE +LLV+   HPH
Sbjct: 4   SQVVNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPH 63

Query: 81  LLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANGTSDASDWEVGECLGMWWRPD 140
           +LLLQI N+  KLPGGRL+P E++ DGLKRKL+ KL  N  +   DW VGEC+  WWRP+
Sbjct: 64  ILLLQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPN 123

Query: 141 FETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENQKTYGPVI 200
           FET++YPY   ++   KEC +L++V L E   F VPKNLKL+AVPL +L++N + YGPVI
Sbjct: 124 FETMMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVI 183

Query: 201 SGVPQLLSKFSFNIIEA 218
           S +PQ LS+F FN+I +
Sbjct: 184 STIPQQLSRFHFNMISS 200

The following BLAST results are available for this feature:

BLAST of MS023689 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154764.1	1.4e-121	100.00	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Momordica charantia]	[more]
KAG6586493.1	6.2e-106	87.50	Pre-mRNA cleavage factor Im 25 kDa subunit 1, partial [Cucurbita argyrosperma su...	[more]
XP_023536755.1	8.1e-106	87.50	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita pepo subsp. pepo]	[more]
XP_022937740.1	5.3e-105	86.57	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita moschata]	[more]
XP_022965443.1	5.3e-105	87.04	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita maxima]	[more]

BLAST of MS023689 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AF0	1.4e-81	65.16	Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8GXS3	2.6e-62	55.84	Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q7T3C6	1.2e-48	47.67	Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955...	[more]
Q3ZCA2	1.6e-48	47.67	Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 ...	[more]
O43809	1.6e-48	47.67	Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=960...	[more]

BLAST of MS023689 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DN47	6.7e-122	100.00	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1HNY2	2.5e-105	87.04	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1FHN4	2.5e-105	86.57	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0LIM0	1.8e-103	83.72	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G0...	[more]
A0A1S3C9Z9	1.0e-101	83.41	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis melo OX=3656 GN=LOC1034986...	[more]

BLAST of MS023689 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29820.1	1.0e-82	65.16	homolog of CFIM-25	[more]
AT4G25550.1	1.8e-63	55.84	Cleavage/polyadenylation specificity factor, 25kDa subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PIRSF	PIRSF017888	CPSF-25	coord: 3..217 e-value: 3.7E-82 score: 272.8
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PFAM	PF13869	NUDIX_2	coord: 23..210 e-value: 9.8E-73 score: 243.7
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PANTHER	PTHR13047	PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT	coord: 10..216
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 22..214 e-value: 1.7E-76 score: 257.9
None	No IPR available	PANTHER	PTHR13047:SF1	PRE-MRNA CLEAVAGE FACTOR IM 25 KDA SUBUNIT 1	coord: 10..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023689.1	MS023689.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006378	mRNA polyadenylation
cellular_component	GO:0005849	mRNA cleavage factor complex
molecular_function	GO:0003729	mRNA binding