MS020604 (gene) Bitter gourd (TR) v1

Overview
NameMS020604
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionTriosephosphate isomerase
Locationscaffold375: 1573462 .. 1577327 (-)
RNA-Seq ExpressionMS020604
SyntenyMS020604
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTGGTGTCCACTTCTCTTGCATCTCAACTATCAGCAGTCCGATTGTCCAGCTCCAAGTCCGACATTTCTCAGACTCTGTTTCAGAACGTTCACTCACAAATCCGCCTTGCCTCCTCCCGGAAAGGTTCCCGTGGCGTTGTCACCATGGCTGGCTCCGGCAAGGTATTTAACCTTTCTCATTAATATGTTATTGATCTGCGCTCGAGCCGTTTCGGCGACCTCAAGAACTATAGATTAGTCTGTCGATCGTCATTCTGGACTTCGTTATTATCTCTTAGAACGAGTGGGAGAGGCAATTAGGAATCGTTGCTTATTTTTTAATTTAATTGTAGGCGGCGGACAATTCGAAAGAGGCGATAATTTTGAGTACTAATCTATTGAAGCTATACATATTACTTTGACGAGTTTATGTATTGATCGTACTTGTGATTAGGGCACGTTTAAGATTTAATTATTATTGTTAACTTTTTGGTTTCATATCTCTCGAACCGTCTAGCGTTTTTGAATAACCATGGAATATACTGATTGGCACATCTTTAATGTTTTTTTTTTTTTCCATTTTTTTTTATTTTCTCTAATACTTAAATTTATAAGGCCATAGTTTTTACGGTATTCTGTACTATCTAATTTGCATTTTTTTTTTCTAACTTCTAATTATTCTCATCTTTTGCTGTATTCGCAGTTCTTCGTTGGTGGAAACTGGAAATGTGTAAGTATATTCATTATCAATAATCATATACCTAAAGTTTTGTGATGGATGATTTTATATGCCAAGCAAAAAAAGTGTGTATGGACGGTGTAAATGCGTGCTATGATTTCTCTTCAGATCATTCCACGCTTCTGTTCTGGCCAAAATCTCACTAGCATGGAACGCAAGTCTTATCACCATATTTCTAGAAGTTAGCTTTTCTGTGAAAACTTGTGTCGAACATAGATGAGGACGTAGTTGACTTTTAATTTTCATCTTTTCATTCATTTTTCTAATATCATATTCACTTACTACTGTCCTGTATTAACTGATTTATTTGAACTTGTACGAAATCTTGTTACTACTAAATGGACGTATGTACAATATTTGAATTAGAAGAAGCCTTAGAAAAATAGATCATGTTCTTCTTTGTCTCTCTATCTTCATTTTCATGGGATGAGGTTTTTAGTTTGAAACTGGTAACTGGTTAGGTTCAAGCTATCCCTCCTGCCAGTTGGCCCAGTTCTATACGAGATACAACTTTTGAATTATTTTGATTCAAGCAACTTTCCTATTTTCAAATACTTAGTCAGTAGGAACCCTTTTTTGTTCAGCATTATTAAGTGTCTAAAGTACACTGGATCCATTACAATACCCAAATAAAGACATCTATGTCTACCTGTCACTGTGGCTTGACCATGATCCAAGATTATTGAACTACTAATATGTCCCCCCCTCCCCATGCTGTATATATATATTTGTAAGTTGATATTGACATGTATTGATTCTTTCCCCATTTGTTTTAATATTCAGAATGGTACAAAGGAATCTATAGCCAAGCTTGTCTCAGACTTGAACAATGCAAAGTTGGAGGATGACGTTGGTAAGTTACGTTTGTTATTGGAGGACGCCTGTCATAAACTTCTTATTTTGTTTGGTACTCAATGTATGTGTTCTTAAGTTAAAAAACAAAGGTCAAGTTTTAGGGTGCGTATCTTCCCCCTCCTGTAATGTATACTATGCTTATTGCTTACTAAAAAACAAAGGTTGCATTTGATACCAGCTCATTCAATTAGAATGAAAACTTAAGCTTGTTTCTGACTTTAATACTTGAATTGGTTTCCTCTTCCTCCTTTTTTAATAATACTTATTCTAAGACCTTTATCAATGTTCTAGTAGGTTGGTAAGTGGTAACATTAAATTATTACTACATCTGAGTTGTTGATGAGGATGAATGACATTTTTGTTTTAAATTCTGATCATCTTTCTCTTCATATTGAAAGCTGGGTTTTCGATAAAAAAATATCTCTTCATATTCATTTTTCTACTCTTTTATGTAGATTATTTTGAAAAAAATAAATAACTTCGGATATGTGTGGGAAAATGCAGATGTTGTTGTAGCACCTCCATTTGTCTACATTGATCAGGTGAAGAGCTCTTTGGCATCTAGGATTGAGATTTCTGCCCAAAACTCATGGGTGAGCAAGGGTGGTGCTTTTACAGGAGAAATCAGGTTATTTGGGTTCCTCTCAATGAGCACATTTTAATTCTCGAGGACCGTTAAAAATTTGCTATCTTAATTCAACTTCTATTGCTGTTGATTATTTTTCTAACATAAAGTTTTCTGAAAAATTATGTTCAGTGCATGTAAACTCTTGACTGTACTGCATTTGACGATGATAACAACTTAAGTGTGCTTGCTTCGTGATCTGCAAATGAGATTATGACAATATTGAACACTAGAATACTAGTGTTTGATTTGATGATACTTAAAATCTTGAACTGCTTTTAGTTGTAGAGTTTATAAATTTTCGTTTTCAAAATTGAGGAGTTTTAGAAATACACGACTCCCTAAGAGATTTAGATGTTTATAAATTAGATAGCTTAAACTTATAATTTTATTTAATTTCAATAGTTAATGATTACTTTTTTGAGGGTTTCGCCTCTTCCAGGGAAGTTTTTTGGGAAGTAAGGATATGATTGATCTAATTGTTGTTTTCATCTGACGCCCACTAAGCTTGTGTTTGTCTTTTTCTTTGGAACTCATACTTGGGGTTTTTCGTATAAATGTGTGTTTTGTGTTTTTCCGCATACAATTATATGTCAGATCTTAGCATTGTTAAAAAAATATTGTGTGCACTGCAGCGTTGAACAATTGAAAGATATAGGCTGCAAGTGGGTTATCCTTGGGCACTCTGAGCGGAGACATATCATTGGTGAAGATGACCAGGTGAATGTTTTGAAATTGAGCTTTAAGTGGTTTCCAATCGATGTTCAAGCTGATTCTCTCCAATGCTGACTGATGCTCTGGCAACTATAGTTTATAGGAAAGAAGGCTGCCTACGCCTTGAGTGAGGGTCTTGGAGTAATAGCCTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTTGATGTGTGCTTTCAACAATTGAAAGCTTTTGCAGGCAAATTGCTGGCTCATTCCTTATTTCACTTTCATGATGGTTATGAAGTCACTGATCCGAGAGCAGAAAAGTGATAGAGTTTTCTGTAACTTCAGTTGTTCCTGTTGACTGCAGATGCTATTCCAAGTTGGGATGGTGTTGTTATTGCATATGAGCCTGTATGGGCAATTGGGACTGGCAAAGTGGCTACACCCGAGCAAGCACAGGAAGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAATATCTCACCAGAAGTTGCATCTAAAACACGAATTATTTATGGAGGTAACTGCAACTTTCATTTGTATTCTGTAATTTACTAGTCAACTTATGGCCATTGTAGAAATAAATTTACTTGTGTTAACAAGTTATTGTGAAGTCCATGTTTCTTAACATTGCCATTGTGATTTATGAGTAACAATCTGGAGCGCCTCACATGTCTAAGGAATGATGTTATTGTTAGGATCTGTGAATGGAAGTAATTGTGCTGAGCTTGCAAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGTGCATTTCTCCTTAGTCCTTATCCCTTTCGCATTTCTAATGTTTTGTAGAAGAAAATTTAGTGAATCAAACACTAAGTTCAGCATCTCAACCTTTTTTCCATGTTGAAATCGCAGGGTCCTGAATTTGGAACGATTGTTAATTCTGTAACCGCCAAGAAAGTTGTTGCT

mRNA sequence

ATGGCGGTGGTGTCCACTTCTCTTGCATCTCAACTATCAGCAGTCCGATTGTCCAGCTCCAAGTCCGACATTTCTCAGACTCTGTTTCAGAACGTTCACTCACAAATCCGCCTTGCCTCCTCCCGGAAAGGTTCCCGTGGCGTTGTCACCATGGCTGGCTCCGGCAAGTTCTTCGTTGGTGGAAACTGGAAATGTAATGGTACAAAGGAATCTATAGCCAAGCTTGTCTCAGACTTGAACAATGCAAAGTTGGAGGATGACGTTGATGTTGTTGTAGCACCTCCATTTGTCTACATTGATCAGGTGAAGAGCTCTTTGGCATCTAGGATTGAGATTTCTGCCCAAAACTCATGGGTGAGCAAGGGTGGTGCTTTTACAGGAGAAATCAGCGTTGAACAATTGAAAGATATAGGCTGCAAGTGGGTTATCCTTGGGCACTCTGAGCGGAGACATATCATTGGTGAAGATGACCAGTTTATAGGAAAGAAGGCTGCCTACGCCTTGAGTGAGGGTCTTGGAGTAATAGCCTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTTGATGTGTGCTTTCAACAATTGAAAGCTTTTGCAGGCAAATTGCTGGCTCATTCCTTATTTCACTTTCATGATGATGCTATTCCAAGTTGGGATGGTGTTGTTATTGCATATGAGCCTGTATGGGCAATTGGGACTGGCAAAGTGGCTACACCCGAGCAAGCACAGGAAGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAATATCTCACCAGAAGTTGCATCTAAAACACGAATTATTTATGGAGGATCTGTGAATGGAAGTAATTGTGCTGAGCTTGCAAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGGTCCTGAATTTGGAACGATTGTTAATTCTGTAACCGCCAAGAAAGTTGTTGCT

Coding sequence (CDS)

ATGGCGGTGGTGTCCACTTCTCTTGCATCTCAACTATCAGCAGTCCGATTGTCCAGCTCCAAGTCCGACATTTCTCAGACTCTGTTTCAGAACGTTCACTCACAAATCCGCCTTGCCTCCTCCCGGAAAGGTTCCCGTGGCGTTGTCACCATGGCTGGCTCCGGCAAGTTCTTCGTTGGTGGAAACTGGAAATGTAATGGTACAAAGGAATCTATAGCCAAGCTTGTCTCAGACTTGAACAATGCAAAGTTGGAGGATGACGTTGATGTTGTTGTAGCACCTCCATTTGTCTACATTGATCAGGTGAAGAGCTCTTTGGCATCTAGGATTGAGATTTCTGCCCAAAACTCATGGGTGAGCAAGGGTGGTGCTTTTACAGGAGAAATCAGCGTTGAACAATTGAAAGATATAGGCTGCAAGTGGGTTATCCTTGGGCACTCTGAGCGGAGACATATCATTGGTGAAGATGACCAGTTTATAGGAAAGAAGGCTGCCTACGCCTTGAGTGAGGGTCTTGGAGTAATAGCCTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTTGATGTGTGCTTTCAACAATTGAAAGCTTTTGCAGGCAAATTGCTGGCTCATTCCTTATTTCACTTTCATGATGATGCTATTCCAAGTTGGGATGGTGTTGTTATTGCATATGAGCCTGTATGGGCAATTGGGACTGGCAAAGTGGCTACACCCGAGCAAGCACAGGAAGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAATATCTCACCAGAAGTTGCATCTAAAACACGAATTATTTATGGAGGATCTGTGAATGGAAGTAATTGTGCTGAGCTTGCAAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGGTCCTGAATTTGGAACGATTGTTAATTCTGTAACCGCCAAGAAAGTTGTTGCT

Protein sequence

MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVGGNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVVA
Homology
BLAST of MS020604 vs. NCBI nr
Match: XP_022146193.1 (triosephosphate isomerase, chloroplastic [Momordica charantia])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 304/319 (95.30%), Postives = 304/319 (95.30%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGV TMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVFTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCFQQLKAFA             DAIPSWDGVVIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFQQLKAFA-------------DAIPSWDGVVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL
Sbjct: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSV AKKVVA
Sbjct: 301 KGPEFGTIVNSVAAKKVVA 306

BLAST of MS020604 vs. NCBI nr
Match: XP_022987010.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 572.8 bits (1475), Expect = 1.9e-159
Identity = 292/319 (91.54%), Postives = 301/319 (94.36%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSA+RLS+SKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI KLVSDLNN KLEDDVDVVVAPPF+YIDQVKSSLA RIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFLYIDQVKSSLAPRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCFQQLKA+A             DA+PSWDG+VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFQQLKAYA-------------DAVPSWDGIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           TP+QAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASL
Sbjct: 241 TPDQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 306

BLAST of MS020604 vs. NCBI nr
Match: KAG7035038.1 (Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.9 bits (1470), Expect = 7.1e-159
Identity = 293/319 (91.85%), Postives = 302/319 (94.67%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS S SDISQTL QNV SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI+KLV+DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGV+ACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCF+QLKAFAGK            A+PSWDG+VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFAGKY-----------AVPSWDGIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           +PEQAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL
Sbjct: 241 SPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 308

BLAST of MS020604 vs. NCBI nr
Match: XP_023512787.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.9 bits (1470), Expect = 7.1e-159
Identity = 291/319 (91.22%), Postives = 300/319 (94.04%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSA+RLS+SKSDISQTLFQNVHSQIRLASSRKG RGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGPRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI KLVSDLNN KLEDDVDVVVAPPF+YIDQVKSSLA RIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFLYIDQVKSSLAPRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCFQQLKA+A             DA+PSWDG+VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFQQLKAYA-------------DAVPSWDGIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           TP+QAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASL
Sbjct: 241 TPDQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 306

BLAST of MS020604 vs. NCBI nr
Match: KAG6605006.1 (Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 569.7 bits (1467), Expect = 1.6e-158
Identity = 293/319 (91.85%), Postives = 301/319 (94.36%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS S SDISQTL QNV SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI+KLV+DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCF+QLKAFA             DA+PSWDG+VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFA-------------DAVPSWDGIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           +PEQAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL
Sbjct: 241 SPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 306

BLAST of MS020604 vs. ExPASy Swiss-Prot
Match: P48496 (Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 PE=1 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 1.5e-135
Identity = 254/335 (75.82%), Postives = 279/335 (83.28%), Query Frame = 0

Query: 1   MAVVSTSLASQL-----------SAVRLSSSKSDIS----QTLFQNVHSQIRL-ASSRKG 60
           MAVVSTSLASQ+           S +R S  K + S     + FQNV S +RL +SSR+ 
Sbjct: 1   MAVVSTSLASQITNPNSAVSTQFSGLRRSFLKLENSVSTQSSFFQNVDSHLRLSSSSRRC 60

Query: 61  SRGVVTMAGSGKFFVGGNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKS 120
            RGVV MAGSGKFFVGGNWKCNGTKESI KLVSDLN+A LE DVDVVVAPPFVYIDQVKS
Sbjct: 61  PRGVVAMAGSGKFFVGGNWKCNGTKESITKLVSDLNSATLEADVDVVVAPPFVYIDQVKS 120

Query: 121 SLASRIEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKA 180
           SL  R+EISAQN W+ KGGAFTGEISVEQLKD+GC+WVILGHSERRH+IGE ++FIGKKA
Sbjct: 121 SLTGRVEISAQNCWIGKGGAFTGEISVEQLKDLGCQWVILGHSERRHVIGEQNEFIGKKA 180

Query: 181 AYALSEGLGVIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGV 240
           AYAL++G+GVIACIGELLEEREAGKTFDVC+QQLKAFA             DA+PSWD V
Sbjct: 181 AYALNQGVGVIACIGELLEEREAGKTFDVCYQQLKAFA-------------DALPSWDNV 240

Query: 241 VIAYEPVWAIGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAEL 300
           V+AYEPVWAIGTGKVA+P+QAQEVH AVRDWLKKN+S EVASKTRIIYGGSVNG NCAEL
Sbjct: 241 VVAYEPVWAIGTGKVASPDQAQEVHVAVRDWLKKNVSEEVASKTRIIYGGSVNGGNCAEL 300

Query: 301 AKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVVA 320
           AK+EDIDGFLVGGASLKGPEF TIVNSVTAKKV A
Sbjct: 301 AKQEDIDGFLVGGASLKGPEFATIVNSVTAKKVAA 322

BLAST of MS020604 vs. ExPASy Swiss-Prot
Match: Q9M4S8 (Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 1.3e-131
Identity = 248/330 (75.15%), Postives = 275/330 (83.33%), Query Frame = 0

Query: 1   MAVVSTSLASQL----------SAVRLSSSKSDIS-QTLFQNVHSQIRLASSRKGSRGVV 60
           MAV STSLASQL          S +R S  K D S  +LFQ++      +SSRK SR VV
Sbjct: 1   MAVASTSLASQLSGPKSLSQPYSGLRRSCPKLDQSHSSLFQHLSLS---SSSRKASRAVV 60

Query: 61  TMAGSGKFFVGGNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASR 120
            MAG+GKFFVGGNWKCNGTK+ I+KLVSDLN+AKLE DVDVVVAPPF+Y+DQVKSSL  R
Sbjct: 61  AMAGTGKFFVGGNWKCNGTKDLISKLVSDLNSAKLEPDVDVVVAPPFLYLDQVKSSLTDR 120

Query: 121 IEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALS 180
           IEIS QNSWV+KGGAFTGEISVEQLKDIG KWVILGHSERRH+IGEDDQFIGKKAAYAL+
Sbjct: 121 IEISGQNSWVAKGGAFTGEISVEQLKDIGRKWVILGHSERRHVIGEDDQFIGKKAAYALN 180

Query: 181 EGLGVIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYE 240
           EGLGVIACIGE LEEREAGKTFDVC++QLKAFA             DA+PSW+ +V+AYE
Sbjct: 181 EGLGVIACIGEKLEEREAGKTFDVCYEQLKAFA-------------DAVPSWENIVVAYE 240

Query: 241 PVWAIGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKED 300
           PVWAIGTGKVA+P+QAQEVH AVR+WLKKN+S EVASKTRIIYGGSVNG N AELAK+ED
Sbjct: 241 PVWAIGTGKVASPQQAQEVHVAVREWLKKNVSAEVASKTRIIYGGSVNGGNSAELAKEED 300

Query: 301 IDGFLVGGASLKGPEFGTIVNSVTAKKVVA 320
           IDGFLVGGASLKGPEF TIVN+VT+KKV A
Sbjct: 301 IDGFLVGGASLKGPEFATIVNAVTSKKVAA 314

BLAST of MS020604 vs. ExPASy Swiss-Prot
Match: Q9SKP6 (Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 6.5e-131
Identity = 244/328 (74.39%), Postives = 265/328 (80.79%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDIS-----QTLFQNVHSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R  S K D +     Q+ F  V+S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKFFVGGNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIE 120
           AGSGKFFVGGNWKCNGTK+SIAKL+SDLN+A LE DVDVVV+PPFVYIDQVKSSL  RI+
Sbjct: 61  AGSGKFFVGGNWKCNGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRID 120

Query: 121 ISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEG 180
           IS QNSWV KGGAFTGEISVEQLKD+GCKWVILGHSERRH+IGE D+FIGKKAAYALSEG
Sbjct: 121 ISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEG 180

Query: 181 LGVIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPV 240
           LGVIACIGE LEEREAGKTFDVCF QLKAFA             DA+PSWD +V+AYEPV
Sbjct: 181 LGVIACIGEKLEEREAGKTFDVCFAQLKAFA-------------DAVPSWDNIVVAYEPV 240

Query: 241 WAIGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDID 300
           WAIGTGKVA+P+QAQEVH AVR WLKKN+S EVASKTRIIYGGSVNG N AELAK+EDID
Sbjct: 241 WAIGTGKVASPQQAQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDID 300

Query: 301 GFLVGGASLKGPEFGTIVNSVTAKKVVA 320
           GFLVGGASLKGPEF TIVNSVT+KKV A
Sbjct: 301 GFLVGGASLKGPEFATIVNSVTSKKVAA 315

BLAST of MS020604 vs. ExPASy Swiss-Prot
Match: P46225 (Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-121
Identity = 216/272 (79.41%), Postives = 235/272 (86.40%), Query Frame = 0

Query: 48  VVTMAGSGKFFVGGNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLA 107
           +V MAGSGKFFVGGNWKCNGTKESI+KLVSDLN A LE DVDVVVAPPF+YIDQVKSSL 
Sbjct: 40  LVAMAGSGKFFVGGNWKCNGTKESISKLVSDLNAATLESDVDVVVAPPFIYIDQVKSSLT 99

Query: 108 SRIEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYA 167
            RIE+SAQN+W+ KGGAFTGEIS EQL DIGC+WVILGHSERRH+IGEDD+FIGKKAAYA
Sbjct: 100 DRIEVSAQNTWIGKGGAFTGEISAEQLVDIGCQWVILGHSERRHVIGEDDEFIGKKAAYA 159

Query: 168 LSEGLGVIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIA 227
           LS+ L V+ACIGELLEEREAGKTFDVCF+Q+KAFA             D I  W  VVIA
Sbjct: 160 LSQNLKVMACIGELLEEREAGKTFDVCFKQMKAFA-------------DNITDWTNVVIA 219

Query: 228 YEPVWAIGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKK 287
           YEPVWAIGTGKVA+PEQAQEVHAAVRDWLK N+S +VAS  RIIYGGSVN +NCAELAKK
Sbjct: 220 YEPVWAIGTGKVASPEQAQEVHAAVRDWLKTNVSADVASTVRIIYGGSVNAANCAELAKK 279

Query: 288 EDIDGFLVGGASLKGPEFGTIVNSVTAKKVVA 320
           EDIDGFLVGGASLKGP+F TI NSVT+KKV A
Sbjct: 280 EDIDGFLVGGASLKGPDFATICNSVTSKKVTA 298

BLAST of MS020604 vs. ExPASy Swiss-Prot
Match: P48497 (Triosephosphate isomerase, cytosolic OS=Stellaria longipes OX=19744 GN=TPI PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 7.8e-100
Identity = 181/266 (68.05%), Postives = 211/266 (79.32%), Query Frame = 0

Query: 56  KFFVGGNWKCNGTKESIAKLVSDLNNAKL--EDDVDVVVAPPFVYIDQVKSSLASRIEIS 115
           KFFVGGNWKCNGT+ES++K+V  LN   +   D V VVV+PP+V++    + L   I+++
Sbjct: 4   KFFVGGNWKCNGTQESVSKIVDTLNEPTIAANDVVTVVVSPPYVFLPDENAELKHEIQVA 63

Query: 116 AQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLG 175
           AQN WV KGGAFTGE+S + L ++G  WVILGHSERR ++GE ++F+GKKAAYA SEGLG
Sbjct: 64  AQNCWVKKGGAFTGEVSAQMLANLGITWVILGHSERRTLLGESNEFVGKKAAYAQSEGLG 123

Query: 176 VIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWA 235
           VIACIGELLEEREAGKTFDVCF+QLK+FA              A P WD VV+AYEPVWA
Sbjct: 124 VIACIGELLEEREAGKTFDVCFKQLKSFA-----------RFPAKP-WDNVVVAYEPVWA 183

Query: 236 IGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGF 295
           IGTGKVA+PEQAQEVH AVRDWLK N+S EVASKTRIIYGGSVNG N   LA +ED+DGF
Sbjct: 184 IGTGKVASPEQAQEVHVAVRDWLKTNVSEEVASKTRIIYGGSVNGGNSLALAAQEDVDGF 243

Query: 296 LVGGASLKGPEFGTIVNSVTAKKVVA 320
           LVGGASLKGPEF TI+NSVTAKKV A
Sbjct: 244 LVGGASLKGPEFATIINSVTAKKVAA 257

BLAST of MS020604 vs. ExPASy TrEMBL
Match: A0A6J1CXY1 (triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015469 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.1e-163
Identity = 304/319 (95.30%), Postives = 304/319 (95.30%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGV TMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVFTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCFQQLKAFA             DAIPSWDGVVIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFQQLKAFA-------------DAIPSWDGVVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL
Sbjct: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSV AKKVVA
Sbjct: 301 KGPEFGTIVNSVAAKKVVA 306

BLAST of MS020604 vs. ExPASy TrEMBL
Match: A0A6J1JFM2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111484585 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.1e-160
Identity = 292/319 (91.54%), Postives = 301/319 (94.36%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSA+RLS+SKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI KLVSDLNN KLEDDVDVVVAPPF+YIDQVKSSLA RIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFLYIDQVKSSLAPRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCFQQLKA+A             DA+PSWDG+VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFQQLKAYA-------------DAVPSWDGIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           TP+QAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASL
Sbjct: 241 TPDQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 306

BLAST of MS020604 vs. ExPASy TrEMBL
Match: A0A6J1G6X6 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111451334 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.7e-158
Identity = 292/319 (91.54%), Postives = 301/319 (94.36%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS S SDISQTL QNV SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI+KLV+DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGV+ACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCF+QLKAFA             DA+PSWDG+VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFA-------------DAVPSWDGIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           +PEQAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL
Sbjct: 241 SPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 306

BLAST of MS020604 vs. ExPASy TrEMBL
Match: A0A6J1FWW5 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111448968 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 2.9e-158
Identity = 290/319 (90.91%), Postives = 299/319 (93.73%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSA+RLS+SKSDISQTLFQNVHSQIRLASSRKG RGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGPRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI KLVSDLNN KLEDDVDVVVAPPF+YIDQV+SSLA RIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFLYIDQVRSSLAPRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCFQQLKA+A             DA+ SWDG+VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFQQLKAYA-------------DAVSSWDGIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           TPEQAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASL
Sbjct: 241 TPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 306

BLAST of MS020604 vs. ExPASy TrEMBL
Match: A0A6J1L7Y2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111500005 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.9e-157
Identity = 290/319 (90.91%), Postives = 299/319 (93.73%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS S SDISQTL QNV SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI KLV+DLNNAKLEDDVD+VVAPPFVYIDQVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVADLNNAKLEDDVDIVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKA+YALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKASYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWAIGTGKVA 240
           LLEEREAGKTFDVCF+QLKAFA             DA+PSWD +VIAYEPVWAIGTGKVA
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFA-------------DAVPSWDDIVIAYEPVWAIGTGKVA 240

Query: 241 TPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300
           +PEQAQEVHAAVRDWLKKN+SPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL
Sbjct: 241 SPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 300

Query: 301 KGPEFGTIVNSVTAKKVVA 320
           KGPEFGTIVNSVTAKKV A
Sbjct: 301 KGPEFGTIVNSVTAKKVAA 306

BLAST of MS020604 vs. TAIR 10
Match: AT2G21170.1 (triosephosphate isomerase )

HSP 1 Score: 468.4 bits (1204), Expect = 4.6e-132
Identity = 244/328 (74.39%), Postives = 265/328 (80.79%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDIS-----QTLFQNVHSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R  S K D +     Q+ F  V+S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKFFVGGNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIE 120
           AGSGKFFVGGNWKCNGTK+SIAKL+SDLN+A LE DVDVVV+PPFVYIDQVKSSL  RI+
Sbjct: 61  AGSGKFFVGGNWKCNGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRID 120

Query: 121 ISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEG 180
           IS QNSWV KGGAFTGEISVEQLKD+GCKWVILGHSERRH+IGE D+FIGKKAAYALSEG
Sbjct: 121 ISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEG 180

Query: 181 LGVIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPV 240
           LGVIACIGE LEEREAGKTFDVCF QLKAFA             DA+PSWD +V+AYEPV
Sbjct: 181 LGVIACIGEKLEEREAGKTFDVCFAQLKAFA-------------DAVPSWDNIVVAYEPV 240

Query: 241 WAIGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDID 300
           WAIGTGKVA+P+QAQEVH AVR WLKKN+S EVASKTRIIYGGSVNG N AELAK+EDID
Sbjct: 241 WAIGTGKVASPQQAQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDID 300

Query: 301 GFLVGGASLKGPEFGTIVNSVTAKKVVA 320
           GFLVGGASLKGPEF TIVNSVT+KKV A
Sbjct: 301 GFLVGGASLKGPEFATIVNSVTSKKVAA 315

BLAST of MS020604 vs. TAIR 10
Match: AT2G21170.2 (triosephosphate isomerase )

HSP 1 Score: 438.3 bits (1126), Expect = 5.1e-123
Identity = 235/328 (71.65%), Postives = 256/328 (78.05%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSKSDIS-----QTLFQNVHSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R  S K D +     Q+ F  V+S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKFFVGGNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIE 120
           AGSGK         NGTK+SIAKL+SDLN+A LE DVDVVV+PPFVYIDQVKSSL  RI+
Sbjct: 61  AGSGK---------NGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRID 120

Query: 121 ISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEG 180
           IS QNSWV KGGAFTGEISVEQLKD+GCKWVILGHSERRH+IGE D+FIGKKAAYALSEG
Sbjct: 121 ISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEG 180

Query: 181 LGVIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPV 240
           LGVIACIGE LEEREAGKTFDVCF QLKAFA             DA+PSWD +V+AYEPV
Sbjct: 181 LGVIACIGEKLEEREAGKTFDVCFAQLKAFA-------------DAVPSWDNIVVAYEPV 240

Query: 241 WAIGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDID 300
           WAIGTGKVA+P+QAQEVH AVR WLKKN+S EVASKTRIIYGGSVNG N AELAK+EDID
Sbjct: 241 WAIGTGKVASPQQAQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDID 300

Query: 301 GFLVGGASLKGPEFGTIVNSVTAKKVVA 320
           GFLVGGASLKGPEF TIVNSVT+KKV A
Sbjct: 301 GFLVGGASLKGPEFATIVNSVTSKKVAA 306

BLAST of MS020604 vs. TAIR 10
Match: AT3G55440.1 (triosephosphate isomerase )

HSP 1 Score: 311.2 bits (796), Expect = 9.5e-85
Identity = 156/263 (59.32%), Postives = 190/263 (72.24%), Query Frame = 0

Query: 56  KFFVGGNWKCNGTKESIAKLVSDLNNAKL--EDDVDVVVAPPFVYIDQVKSSLASRIEIS 115
           KFFVGGNWKCNGT E + K+V+ LN A++  +D V+VVV+PP+V++  VKS+L S   ++
Sbjct: 4   KFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDFFVA 63

Query: 116 AQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLG 175
           AQN WV KGGAFTGE+S E L ++   WVILGHSERR I+ E  +F+G K AYAL++GL 
Sbjct: 64  AQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQGLK 123

Query: 176 VIACIGELLEEREAGKTFDVCFQQLKAFAGKLLAHSLFHFHDDAIPSWDGVVIAYEPVWA 235
           VIAC+GE LEEREAG T DV   Q KA A             D + +W  VVIAYEPVWA
Sbjct: 124 VIACVGETLEEREAGSTMDVVAAQTKAIA-------------DRVTNWSNVVIAYEPVWA 183

Query: 236 IGTGKVATPEQAQEVHAAVRDWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGF 295
           IGTGKVA+P QAQEVH  +R WL KN+S +VA+ TRIIYGGSVNG NC EL  + D+DGF
Sbjct: 184 IGTGKVASPAQAQEVHDELRKWLAKNVSADVAATTRIIYGGSVNGGNCKELGGQADVDGF 243

Query: 296 LVGGASLKGPEFGTIVNSVTAKK 317
           LVGGASLK PEF  I+ +   KK
Sbjct: 244 LVGGASLK-PEFIDIIKAAEVKK 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146193.11.1e-16295.30triosephosphate isomerase, chloroplastic [Momordica charantia][more]
XP_022987010.11.9e-15991.54triosephosphate isomerase, chloroplastic-like [Cucurbita maxima][more]
KAG7035038.17.1e-15991.85Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_023512787.17.1e-15991.22triosephosphate isomerase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6605006.11.6e-15891.85Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
P484961.5e-13575.82Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 P... [more]
Q9M4S81.3e-13175.15Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=... [more]
Q9SKP66.5e-13174.39Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM ... [more]
P462251.2e-12179.41Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1[more]
P484977.8e-10068.05Triosephosphate isomerase, cytosolic OS=Stellaria longipes OX=19744 GN=TPI PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CXY15.1e-16395.30triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1JFM29.1e-16091.54triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1G6X61.7e-15891.54triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1FWW52.9e-15890.91triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1L7Y21.9e-15790.91triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G21170.14.6e-13274.39triosephosphate isomerase [more]
AT2G21170.25.1e-12371.65triosephosphate isomerase [more]
AT3G55440.19.5e-8559.32triosephosphate isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 30..318
e-value: 5.4E-113
score: 378.6
IPR000652Triosephosphate isomerasePFAMPF00121TIMcoord: 58..309
e-value: 1.2E-87
score: 293.4
IPR000652Triosephosphate isomeraseTIGRFAMTIGR00419TIGR00419coord: 58..301
e-value: 2.8E-79
score: 264.1
IPR000652Triosephosphate isomerasePANTHERPTHR21139TRIOSEPHOSPHATE ISOMERASEcoord: 4..317
IPR000652Triosephosphate isomerasePROSITEPS51440TIM_2coord: 56..311
score: 82.269646
IPR000652Triosephosphate isomeraseCDDcd00311TIMcoord: 57..310
e-value: 1.01414E-127
score: 362.624
NoneNo IPR availablePANTHERPTHR21139:SF20TRIOSEPHOSPHATE ISOMERASE, CHLOROPLASTICcoord: 4..317
IPR020861Triosephosphate isomerase, active sitePROSITEPS00171TIM_1coord: 227..237
IPR022896Triosephosphate isomerase, bacterial/eukaryoticHAMAPMF_00147_BTIM_Bcoord: 55..311
score: 57.882378
IPR035990Triosephosphate isomerase superfamilySUPERFAMILY51351Triosephosphate isomerase (TIM)coord: 53..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020604.1MS020604.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004807 triose-phosphate isomerase activity