Homology
BLAST of MS013417 vs. NCBI nr
Match:
XP_022140040.1 (putative pectinesterase/pectinesterase inhibitor 24 [Momordica charantia])
HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 564/568 (99.30%), Postives = 565/568 (99.47%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD--GN 60
MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD GN
Sbjct: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
Query: 61 GNGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADY 120
GNGNSALSSSIKAVCDVTLYPETCNYAFGPL NSSRLDPGQICKLSVQLALGELSRAADY
Sbjct: 61 GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQS 240
QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYE+HQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 567
TAKKFTVSSFIDGKDWISKAQVPFKSGL
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568
BLAST of MS013417 vs. NCBI nr
Match:
XP_038895610.1 (putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida])
HSP 1 Score: 973.4 bits (2515), Expect = 8.4e-280
Identity = 494/567 (87.13%), Postives = 525/567 (92.59%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGS-KDDGNG 60
MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLV VVVAVVFGTRN S +D +
Sbjct: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60
Query: 61 NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 120
N + ALS+SIKAVCDVTLYP TC AFGPL NSS LDP QI KLSVQLALGELSR ADY+
Sbjct: 61 NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120
Query: 121 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQ 180
F HAITNSTDN+T LAL++CHELLDLALDHLNISLSS++ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
Query: 181 QTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSS 240
QTCI+GL EVDP L N IANYLKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYEDHQS+
Sbjct: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240
Query: 241 ADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 300
DWARPILRKLAQRD+RKQADIVVAKD SGKYKTI+AAL+AVP+KSKK TVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300
Query: 301 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 360
ENVEV KNKWNVV++GDGM AT VT LN IDGTPTFS+ATFAAKGKGFIAIDMGF+N A
Sbjct: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360
Query: 361 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 420
GP+KHQAVALMSTSDQSIFYRC+MDAYQDTLY+HSNRQFYREC V GTVDFIFGNSAVVL
Sbjct: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420
Query: 421 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 480
QNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQDSSI PYENLK TETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480
Query: 481 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKT 540
TTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKW GV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540
Query: 541 AKKFTVSSFIDGKDWISKAQVPFKSGL 567
AKKFTVSSFIDGKDWISKAQVPFK+ +
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 567
BLAST of MS013417 vs. NCBI nr
Match:
XP_023000829.1 (probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima])
HSP 1 Score: 951.4 bits (2458), Expect = 3.4e-273
Identity = 481/566 (84.98%), Postives = 514/566 (90.81%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1 MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
Query: 61 GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
N ALS+SIKAVCD TLYP+TCN AFGPL NSS LDPGQI K SVQLAL ELSR ADY+
Sbjct: 61 HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120
Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
+HAITNSTDN+T LAL CHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180
Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
TCI+GLEEVD L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED S+
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ ALKAV DKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300
Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
NVEV KNKWNVVMIGDGM +TTVT KLNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK T+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480
Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
TVFMQN GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
KKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566
BLAST of MS013417 vs. NCBI nr
Match:
KAG6583848.1 (putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 948.0 bits (2449), Expect = 3.8e-272
Identity = 480/566 (84.81%), Postives = 513/566 (90.64%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1 MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
Query: 61 GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
N ALS+SIKAVCD TLYP+TC AFG L NSS LDPGQI K SVQLALGELSR ADY+
Sbjct: 61 HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
+HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAMDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
TCI+GLEEVD L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED S+
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
NVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
TVFMQN GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
KKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566
BLAST of MS013417 vs. NCBI nr
Match:
XP_022927225.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata])
HSP 1 Score: 948.0 bits (2449), Expect = 3.8e-272
Identity = 480/566 (84.81%), Postives = 513/566 (90.64%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1 MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
Query: 61 GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
N ALS+SIKAVCD TLYP+TC AFG L NSS LDPGQI K SVQLALGELSR ADY+
Sbjct: 61 HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
+HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
TCI+GLEEVD L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED S+
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
NVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
TVFMQN GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
KKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566
BLAST of MS013417 vs. ExPASy Swiss-Prot
Match:
Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)
HSP 1 Score: 593.6 bits (1529), Expect = 2.4e-168
Identity = 313/570 (54.91%), Postives = 407/570 (71.40%), Query Frame = 0
Query: 6 PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKD----DGNGNG 65
PYGKVDE + +RL+A R+TRK IA+I +S V+L +V+ VFGT K + N NG
Sbjct: 4 PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63
Query: 66 NSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFK 125
+S +S S+KAVCDVTL+ E C G N+S L+P ++ + +V++ + E+S+A
Sbjct: 64 DS-ISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI----- 123
Query: 126 HAITNST-DNRTKLALESCHELLDLALDHLNISLSST---EITLLKAVDDLKTWLTSAAT 185
+A ++S D + + + +C ELLDL +D+LN +L+S+ ++T+ + VDDL+TWL+SA T
Sbjct: 124 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGT 183
Query: 186 YQQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-RRLMSYEDH 245
YQ+TC+E L P +R ++LKNSTELTSN LAI+++ KI DS L RRL++ D
Sbjct: 184 YQRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADV 243
Query: 246 QSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKG 305
+ R R L DLRK ADIVVAKDGSGKY+TI AL+ VP+KS+K T+IYVKKG
Sbjct: 244 EVDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKG 303
Query: 306 TYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQ 365
Y ENV+V K WNV+++GDG + + V+ +LN IDGTPTF +ATFA GKGF+A DMGF
Sbjct: 304 VYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFI 363
Query: 366 NIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSA 425
N AGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYRECT+ GTVDFIFGNSA
Sbjct: 364 NTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSA 423
Query: 426 VVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWK 485
VLQ+C ILP+ P+ GQ+NTITAQGR DPN NTGISI +I+P +L T+LGRPWK
Sbjct: 424 SVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWK 483
Query: 486 NYSTTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNID 545
N+STTV M +YL I GWLPW G SAPDTIFY E++N GPG+ST RVKWKG+R +
Sbjct: 484 NFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLS 543
Query: 546 NKTAKKFTVSSFIDGKDWISKAQVPFKSGL 567
K A +FTV FIDG W+ +VPF+SGL
Sbjct: 544 TKEANRFTVKPFIDGGRWLPATKVPFRSGL 561
BLAST of MS013417 vs. ExPASy Swiss-Prot
Match:
Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)
HSP 1 Score: 585.1 bits (1507), Expect = 8.5e-166
Identity = 304/566 (53.71%), Postives = 402/566 (71.02%), Query Frame = 0
Query: 7 YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT--RNGSKDDGNGNGNSA 66
YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV V GT R+ SK N
Sbjct: 4 YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63
Query: 67 LSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 126
+S S+KA+CDVTL+ E C G N+SR P ++ K +V++ + ELS+ D +
Sbjct: 64 ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123
Query: 127 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQTCIE 186
DN T A+ +C EL+ LA+D LN +++S+ LK DDL+TWL+S TYQ+TC++
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183
Query: 187 GLEEVD-PTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSADWA 246
L E + P+L N+LKNSTE+TSN LAI+++ KI D++ RR E + A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243
Query: 247 -RPIL---RKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 306
P++ R L DL+K+A IVVAKDGSGKY+TI AL V +K++K T+IYVKKG Y
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303
Query: 307 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 366
ENV V K KWNVVM+GDG + T V+A LNFIDGTPTF +ATFA GKGF+A DMGF N A
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363
Query: 367 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 426
GP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423
Query: 427 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 486
Q C ILP+ P+ GQ+NTITAQGR DPNQNTGISI + +I P +NL +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483
Query: 487 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVR-NIDNK 546
TTV M++++ I P GWLPW G +APDTIFYAE+ N GPG+ST RVKW+G++ ++ K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543
Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKS 565
A KFTV FIDG +W+ +VPF S
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562
BLAST of MS013417 vs. ExPASy Swiss-Prot
Match:
Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)
HSP 1 Score: 487.3 bits (1253), Expect = 2.4e-136
Identity = 268/582 (46.05%), Postives = 373/582 (64.09%), Query Frame = 0
Query: 7 YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFG-------TRNGSKDDGNG 66
YGKV+E +Q + ++TRKR+ +I +SS+VL+AV++A V G + + D
Sbjct: 10 YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69
Query: 67 NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 126
+ ++S+KAVCD T YP +C + L S+ DP + KLS+++A+ ELS +
Sbjct: 70 QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELS---SFP 129
Query: 127 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSST-----EITLLKAVDDLKTWLTS 186
K D R + A++ C + ALD LN S+S+ I +V +++TWL++
Sbjct: 130 SKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189
Query: 187 AATYQQTCIEGLEEVDPTLR----NSIANYLKNSTELTSNGLAIVS----FFSKITDSLN 246
A T Q TC++ + E++ T I ++NSTE SN LAIV+ S+ ++
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249
Query: 247 LRRLMSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSK 306
RRL+ + + +A+ R L +++ D VVAKDGSG++KTI ALK V KS+
Sbjct: 250 HRRLLGFPEWLGAAE-----RRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSE 309
Query: 307 KSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGK 366
+ +YVK+G Y EN+++ KN WNV++ GDG + T V NF+DGTPTF +ATFA KGK
Sbjct: 310 ERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGK 369
Query: 367 GFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEG 426
GFIA D+GF N AG SKHQAVAL S SD+S+F+RC D +QDTLYAHSNRQFYR+C + G
Sbjct: 370 GFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITG 429
Query: 427 TVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKA 486
T+DFIFGN+AVV Q+C I+P+ PL Q NTITAQG+ DPNQNTGI IQ S+ITP+ N
Sbjct: 430 TIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLT 489
Query: 487 TETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSSTAK 546
TYLGRPWK++STTV MQ+ +G+L+ P GW+ WV P TIFYAE+QN GPG+ ++
Sbjct: 490 APTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQ 549
Query: 547 RVKWKGVR-NIDNKTAKKFTVSSFIDGKDWISKAQVPFKSGL 567
RVKW G + I ++ A++FTV SFI G +W+ A V F S L
Sbjct: 550 RVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581
BLAST of MS013417 vs. ExPASy Swiss-Prot
Match:
Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)
HSP 1 Score: 452.2 bits (1162), Expect = 8.6e-126
Identity = 254/573 (44.33%), Postives = 362/573 (63.18%), Query Frame = 0
Query: 7 YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT---RNGSKDDGNGNGNS 66
YGKVDE L L ++TRKR+ ++ +S VVL+AV++A V T +N ++ +
Sbjct: 10 YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69
Query: 67 ALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHA 126
S+S+KA+C VT +PE+C + L +S+ DP + KLS+++ + EL +D K +
Sbjct: 70 TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLS 129
Query: 127 ITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLK------AVDDLKTWLTSAAT 186
+ D R K AL C +L++ ALD LN ++S+ + K ++DLKTWL++ T
Sbjct: 130 -KETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189
Query: 187 YQQTCIEGLEEV--------DPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-- 246
+TC + L+E+ + T+ ++ + + STE TSN LAIVS L +
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249
Query: 247 ---RRLMSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDK 306
RRLMS+ HQ S D+ + R+L Q K D+ VA DG+G T++ A+ VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309
Query: 307 SKKSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAK 366
S K VIYVK GTY ENV + K+KWNV++ GDG T ++ NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369
Query: 367 GKGFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTV 426
GKGFI D+G N AG +KHQAVA S SD S++Y+C D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429
Query: 427 EGTVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENL 486
GT+DFIFG++AVV Q C I+P+ PL Q NTITAQG+ DPNQ++G+SIQ +I+ N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489
Query: 487 KATETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
A TYLGRPWK +STTV M+ +G++++P+GW+ WV G P +I Y E++N GPGS
Sbjct: 490 IA-PTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549
Query: 547 AKRVKWKGVRNI-DNKTAKKFTVSSFIDGKDWI 556
+RVKW G + + + A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576
BLAST of MS013417 vs. ExPASy Swiss-Prot
Match:
Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)
HSP 1 Score: 402.5 bits (1033), Expect = 7.8e-111
Identity = 235/564 (41.67%), Postives = 343/564 (60.82%), Query Frame = 0
Query: 19 DASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNSALSSSIKAVCDV--- 78
D S+R R+ I VI +SSV+L+++VVAV G + +K DG+ G + +++S+KAV DV
Sbjct: 7 DESKRKRRYI-VITISSVLLISMVVAVTVGV-SLNKHDGDSKGKAEVNASVKAVKDVCAP 66
Query: 79 TLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAITNSTDNRTKLA 138
T Y +TC + DP ++ K + + + +++ AA + + D+RT++A
Sbjct: 67 TDYRKTCEDTL-IKNGKNTTDPMELVKTAFNVTMKQITDAAK-KSQTIMELQKDSRTRMA 126
Query: 139 LESCHELLDLALDHLNIS---LSSTEITLL-KAVDDLKTWLTSAATYQQTCIEGLEEVDP 198
L+ C EL+D ALD L+ S L E LL +A+ +L+ WL++A ++++TC+EG +
Sbjct: 127 LDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQG 186
Query: 199 TLRNSIANYLKNSTELTSNGLAIVSFFSKIT-----DSLNLRRLMSYEDHQSSADWARPI 258
++ LK + ELT NGLAI+S S LN RRL++ E S D
Sbjct: 187 NAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVDQRGRK 246
Query: 259 LRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKENVEVGK 318
L + A + DIVVA+DGSG+YKTI+ AL+ VP K + V+++K G YKE V+V K
Sbjct: 247 LLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 306
Query: 319 NKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAGPSKHQA 378
++V IGDG + T ++ N+ DG T+ +AT A G FIA ++GF+N AG KHQA
Sbjct: 307 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 366
Query: 379 VALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQNCTILP 438
VA+ SD+SIF+ C+ D YQDTLY HS+RQF+R+CT+ GT+DF+FG++A V QNCT+L
Sbjct: 367 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 426
Query: 439 KLPLLGQKNTITAQGRFDPNQNTGISIQDSSIT---PYENLKAT-ETYLGRPWKNYSTTV 498
+ PL Q ITA GR DP ++TG Q +I Y +K T + YLGRPWK YS T+
Sbjct: 427 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 486
Query: 499 FMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTAKK 558
M ++ +QP GW PW+G T+FY+E QN GPGS+ A RV W G++ + + K
Sbjct: 487 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 546
Query: 559 FTVSSFIDGKDWISKAQVPFKSGL 567
FT + +I G DWI VP+ +GL
Sbjct: 547 FTPAQYIQGDDWIPGKGVPYTTGL 565
BLAST of MS013417 vs. ExPASy TrEMBL
Match:
A0A6J1CDY3 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010791 PE=3 SV=1)
HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 564/568 (99.30%), Postives = 565/568 (99.47%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD--GN 60
MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDD GN
Sbjct: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
Query: 61 GNGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADY 120
GNGNSALSSSIKAVCDVTLYPETCNYAFGPL NSSRLDPGQICKLSVQLALGELSRAADY
Sbjct: 61 GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQS 240
QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYE+HQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 567
TAKKFTVSSFIDGKDWISKAQVPFKSGL
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568
BLAST of MS013417 vs. ExPASy TrEMBL
Match:
A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)
HSP 1 Score: 951.4 bits (2458), Expect = 1.7e-273
Identity = 481/566 (84.98%), Postives = 514/566 (90.81%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1 MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
Query: 61 GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
N ALS+SIKAVCD TLYP+TCN AFGPL NSS LDPGQI K SVQLAL ELSR ADY+
Sbjct: 61 HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120
Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
+HAITNSTDN+T LAL CHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180
Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
TCI+GLEEVD L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED S+
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ ALKAV DKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300
Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
NVEV KNKWNVVMIGDGM +TTVT KLNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK T+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480
Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
TVFMQN GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
KKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566
BLAST of MS013417 vs. ExPASy TrEMBL
Match:
A0A6J1ENB0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1)
HSP 1 Score: 948.0 bits (2449), Expect = 1.8e-272
Identity = 480/566 (84.81%), Postives = 513/566 (90.64%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D + N
Sbjct: 1 MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
Query: 61 GNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVF 120
N ALS+SIKAVCD TLYP+TC AFG L NSS LDPGQI K SVQLALGELSR ADY+
Sbjct: 61 HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
Query: 121 KHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQ 180
+HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
Query: 181 TCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSA 240
TCI+GLEEVD L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYED S+
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
Query: 241 DWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKE 300
DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG YKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
Query: 301 NVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAG 360
NVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N AG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
Query: 361 PSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQ 420
PSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
Query: 421 NCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYST 480
NC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
Query: 481 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 540
TVFMQN GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
Query: 541 KKFTVSSFIDGKDWISKAQVPFKSGL 567
KKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566
BLAST of MS013417 vs. ExPASy TrEMBL
Match:
A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)
HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 477/567 (84.13%), Postives = 517/567 (91.18%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGS-KDDGNG 60
MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLVAVVVAVVFGTRN S +D +
Sbjct: 1 MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
Query: 61 NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 120
N N +LS+SIKAVCDVTLYP+TC AFGP NSS LDPGQI KL+VQLALGELS+ AD++
Sbjct: 61 NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120
Query: 121 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQ 180
F HAITN+TD++T LAL++CHELLDLALDHLNI+LSS++ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180
Query: 181 QTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSS 240
QTCI+GL EVDP L + AN+LKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYED QS+
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240
Query: 241 ADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 300
DWARPILRKLAQ D+RKQADIVVAKD SGKYKTI+ ALKAVPDKSKK TVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
Query: 301 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 360
E VEV KNKWNVVM+GDGMNAT V+ LN IDGTPTFS+A+FAAKGKGFIAIDMGF N A
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360
Query: 361 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 420
GPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYREC V GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
Query: 421 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 480
QNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQ S I P++NLK TETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480
Query: 481 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKT 540
TTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKWKGV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540
Query: 541 AKKFTVSSFIDGKDWISKAQVPFKSGL 567
AKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567
BLAST of MS013417 vs. ExPASy TrEMBL
Match:
A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)
HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 477/567 (84.13%), Postives = 517/567 (91.18%), Query Frame = 0
Query: 1 MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGS-KDDGNG 60
MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLVAVVVAVVFGTRN S +D +
Sbjct: 1 MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
Query: 61 NGNSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYV 120
N N +LS+SIKAVCDVTLYP+TC AFGP NSS LDPGQI KL+VQLALGELS+ AD++
Sbjct: 61 NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120
Query: 121 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQ 180
F HAITN+TD++T LAL++CHELLDLALDHLNI+LSS++ITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180
Query: 181 QTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSS 240
QTCI+GL EVDP L + AN+LKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYED QS+
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240
Query: 241 ADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 300
DWARPILRKLAQ D+RKQADIVVAKD SGKYKTI+ ALKAVPDKSKK TVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
Query: 301 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 360
E VEV KNKWNVVM+GDGMNAT V+ LN IDGTPTFS+A+FAAKGKGFIAIDMGF N A
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360
Query: 361 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 420
GPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYREC V GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
Query: 421 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 480
QNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQ S I P++NLK TETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480
Query: 481 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKT 540
TTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKWKGV+NID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540
Query: 541 AKKFTVSSFIDGKDWISKAQVPFKSGL 567
AKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567
BLAST of MS013417 vs. TAIR 10
Match:
AT3G10710.1 (root hair specific 12 )
HSP 1 Score: 593.6 bits (1529), Expect = 1.7e-169
Identity = 313/570 (54.91%), Postives = 407/570 (71.40%), Query Frame = 0
Query: 6 PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKD----DGNGNG 65
PYGKVDE + +RL+A R+TRK IA+I +S V+L +V+ VFGT K + N NG
Sbjct: 4 PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63
Query: 66 NSALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFK 125
+S +S S+KAVCDVTL+ E C G N+S L+P ++ + +V++ + E+S+A
Sbjct: 64 DS-ISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI----- 123
Query: 126 HAITNST-DNRTKLALESCHELLDLALDHLNISLSST---EITLLKAVDDLKTWLTSAAT 185
+A ++S D + + + +C ELLDL +D+LN +L+S+ ++T+ + VDDL+TWL+SA T
Sbjct: 124 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGT 183
Query: 186 YQQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-RRLMSYEDH 245
YQ+TC+E L P +R ++LKNSTELTSN LAI+++ KI DS L RRL++ D
Sbjct: 184 YQRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADV 243
Query: 246 QSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKG 305
+ R R L DLRK ADIVVAKDGSGKY+TI AL+ VP+KS+K T+IYVKKG
Sbjct: 244 EVDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKG 303
Query: 306 TYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQ 365
Y ENV+V K WNV+++GDG + + V+ +LN IDGTPTF +ATFA GKGF+A DMGF
Sbjct: 304 VYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFI 363
Query: 366 NIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSA 425
N AGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYRECT+ GTVDFIFGNSA
Sbjct: 364 NTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSA 423
Query: 426 VVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWK 485
VLQ+C ILP+ P+ GQ+NTITAQGR DPN NTGISI +I+P +L T+LGRPWK
Sbjct: 424 SVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWK 483
Query: 486 NYSTTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNID 545
N+STTV M +YL I GWLPW G SAPDTIFY E++N GPG+ST RVKWKG+R +
Sbjct: 484 NFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLS 543
Query: 546 NKTAKKFTVSSFIDGKDWISKAQVPFKSGL 567
K A +FTV FIDG W+ +VPF+SGL
Sbjct: 544 TKEANRFTVKPFIDGGRWLPATKVPFRSGL 561
BLAST of MS013417 vs. TAIR 10
Match:
AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 585.1 bits (1507), Expect = 6.0e-167
Identity = 304/566 (53.71%), Postives = 402/566 (71.02%), Query Frame = 0
Query: 7 YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT--RNGSKDDGNGNGNSA 66
YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV V GT R+ SK N
Sbjct: 4 YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63
Query: 67 LSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 126
+S S+KA+CDVTL+ E C G N+SR P ++ K +V++ + ELS+ D +
Sbjct: 64 ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123
Query: 127 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQTCIE 186
DN T A+ +C EL+ LA+D LN +++S+ LK DDL+TWL+S TYQ+TC++
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183
Query: 187 GLEEVD-PTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYEDHQSSADWA 246
L E + P+L N+LKNSTE+TSN LAI+++ KI D++ RR E + A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243
Query: 247 -RPIL---RKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 306
P++ R L DL+K+A IVVAKDGSGKY+TI AL V +K++K T+IYVKKG Y
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303
Query: 307 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 366
ENV V K KWNVVM+GDG + T V+A LNFIDGTPTF +ATFA GKGF+A DMGF N A
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363
Query: 367 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 426
GP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423
Query: 427 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 486
Q C ILP+ P+ GQ+NTITAQGR DPNQNTGISI + +I P +NL +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483
Query: 487 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVR-NIDNK 546
TTV M++++ I P GWLPW G +APDTIFYAE+ N GPG+ST RVKW+G++ ++ K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543
Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKS 565
A KFTV FIDG +W+ +VPF S
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562
BLAST of MS013417 vs. TAIR 10
Match:
AT1G53840.1 (pectin methylesterase 1 )
HSP 1 Score: 452.2 bits (1162), Expect = 6.1e-127
Identity = 254/573 (44.33%), Postives = 362/573 (63.18%), Query Frame = 0
Query: 7 YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT---RNGSKDDGNGNGNS 66
YGKVDE L L ++TRKR+ ++ +S VVL+AV++A V T +N ++ +
Sbjct: 10 YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69
Query: 67 ALSSSIKAVCDVTLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHA 126
S+S+KA+C VT +PE+C + L +S+ DP + KLS+++ + EL +D K +
Sbjct: 70 TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLS 129
Query: 127 ITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLK------AVDDLKTWLTSAAT 186
+ D R K AL C +L++ ALD LN ++S+ + K ++DLKTWL++ T
Sbjct: 130 -KETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189
Query: 187 YQQTCIEGLEEV--------DPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-- 246
+TC + L+E+ + T+ ++ + + STE TSN LAIVS L +
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249
Query: 247 ---RRLMSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDK 306
RRLMS+ HQ S D+ + R+L Q K D+ VA DG+G T++ A+ VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309
Query: 307 SKKSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAK 366
S K VIYVK GTY ENV + K+KWNV++ GDG T ++ NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369
Query: 367 GKGFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTV 426
GKGFI D+G N AG +KHQAVA S SD S++Y+C D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429
Query: 427 EGTVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENL 486
GT+DFIFG++AVV Q C I+P+ PL Q NTITAQG+ DPNQ++G+SIQ +I+ N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489
Query: 487 KATETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
A TYLGRPWK +STTV M+ +G++++P+GW+ WV G P +I Y E++N GPGS
Sbjct: 490 IA-PTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549
Query: 547 AKRVKWKGVRNI-DNKTAKKFTVSSFIDGKDWI 556
+RVKW G + + + A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576
BLAST of MS013417 vs. TAIR 10
Match:
AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 402.5 bits (1033), Expect = 5.5e-112
Identity = 235/564 (41.67%), Postives = 343/564 (60.82%), Query Frame = 0
Query: 19 DASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNSALSSSIKAVCDV--- 78
D S+R R+ I VI +SSV+L+++VVAV G + +K DG+ G + +++S+KAV DV
Sbjct: 7 DESKRKRRYI-VITISSVLLISMVVAVTVGV-SLNKHDGDSKGKAEVNASVKAVKDVCAP 66
Query: 79 TLYPETCNYAFGPLYNSSRLDPGQICKLSVQLALGELSRAADYVFKHAITNSTDNRTKLA 138
T Y +TC + DP ++ K + + + +++ AA + + D+RT++A
Sbjct: 67 TDYRKTCEDTL-IKNGKNTTDPMELVKTAFNVTMKQITDAAK-KSQTIMELQKDSRTRMA 126
Query: 139 LESCHELLDLALDHLNIS---LSSTEITLL-KAVDDLKTWLTSAATYQQTCIEGLEEVDP 198
L+ C EL+D ALD L+ S L E LL +A+ +L+ WL++A ++++TC+EG +
Sbjct: 127 LDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQG 186
Query: 199 TLRNSIANYLKNSTELTSNGLAIVSFFSKIT-----DSLNLRRLMSYEDHQSSADWARPI 258
++ LK + ELT NGLAI+S S LN RRL++ E S D
Sbjct: 187 NAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVDQRGRK 246
Query: 259 LRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKENVEVGK 318
L + A + DIVVA+DGSG+YKTI+ AL+ VP K + V+++K G YKE V+V K
Sbjct: 247 LLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 306
Query: 319 NKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAGPSKHQA 378
++V IGDG + T ++ N+ DG T+ +AT A G FIA ++GF+N AG KHQA
Sbjct: 307 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 366
Query: 379 VALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQNCTILP 438
VA+ SD+SIF+ C+ D YQDTLY HS+RQF+R+CT+ GT+DF+FG++A V QNCT+L
Sbjct: 367 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 426
Query: 439 KLPLLGQKNTITAQGRFDPNQNTGISIQDSSIT---PYENLKAT-ETYLGRPWKNYSTTV 498
+ PL Q ITA GR DP ++TG Q +I Y +K T + YLGRPWK YS T+
Sbjct: 427 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 486
Query: 499 FMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTAKK 558
M ++ +QP GW PW+G T+FY+E QN GPGS+ A RV W G++ + + K
Sbjct: 487 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 546
Query: 559 FTVSSFIDGKDWISKAQVPFKSGL 567
FT + +I G DWI VP+ +GL
Sbjct: 547 FTPAQYIQGDDWIPGKGVPYTTGL 565
BLAST of MS013417 vs. TAIR 10
Match:
AT3G14300.1 (pectinesterase family protein )
HSP 1 Score: 394.4 bits (1012), Expect = 1.5e-109
Identity = 231/512 (45.12%), Postives = 308/512 (60.16%), Query Frame = 0
Query: 66 SSSIKAVCDVTLYPETCNYAFGPL-YNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 125
SS ++ VC+VT YP +C + L + + DP + +LS+Q+ EL+ K A
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVGLPKKLA- 515
Query: 126 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITL--------LKAVDDLKTWLTSAA 185
+ D K AL C ++ DLA+D +N ++SS + + + DL TWL+SA
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575
Query: 186 TYQQTCIEGLEE--VDPTLRNSIANYLKNSTELTSNGLAIVSFF----SKITDSLNLRRL 245
T TC + L+E + + + + + NSTE TSN LAIV+ SK + RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635
Query: 246 MSYEDHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTV 305
+ + S +W RP +R+L Q + VA DGSG +T++ A+ VP K K V
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFV 695
Query: 306 IYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIA 365
IYVK GTY ENV + K+KWNV + GDG + T ++ N +DG TF+++TFA +GKGF+
Sbjct: 696 IYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMM 755
Query: 366 IDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDF 425
DMG N AGP KHQAVA S SD+S++YRC D YQDTLY HSNRQ+YR C V GTVDF
Sbjct: 756 KDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDF 815
Query: 426 IFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETY 485
IFG VV Q C+I P+ PL Q NTITA+G + NQNTGISI +I+P N+ AT TY
Sbjct: 816 IFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNVTAT-TY 875
Query: 486 LGRPWKNYSTTVFMQNYLGSLIQPTGWLPWVGT--SAPDTIFYAEFQNYGPGSSTAKRVK 545
LGRPWK +S TV MQ+ +GS + P GW+ W T P TIFY E++N GPGS +KRVK
Sbjct: 876 LGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVK 935
Query: 546 WKGVRNI-DNKTAKKFTVSSFIDGKD-WISKA 559
W G + I + A +FTV F+ G D WI KA
Sbjct: 936 WAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022140040.1 | 0.0e+00 | 99.30 | putative pectinesterase/pectinesterase inhibitor 24 [Momordica charantia] | [more] |
| XP_038895610.1 | 8.4e-280 | 87.13 | putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida] | [more] |
| XP_023000829.1 | 3.4e-273 | 84.98 | probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima] | [more] |
| KAG6583848.1 | 3.8e-272 | 84.81 | putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosp... | [more] |
| XP_022927225.1 | 3.8e-272 | 84.81 | putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9SG77 | 2.4e-168 | 54.91 | Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3... | [more] |
| Q9FF78 | 8.5e-166 | 53.71 | Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3... | [more] |
| Q43111 | 2.4e-136 | 46.05 | Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1 | [more] |
| Q43867 | 8.6e-126 | 44.33 | Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1 | [more] |
| Q8GX86 | 7.8e-111 | 41.67 | Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CDY3 | 0.0e+00 | 99.30 | Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010791 PE=3 SV=1 | [more] |
| A0A6J1KL23 | 1.7e-273 | 84.98 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1 | [more] |
| A0A6J1ENB0 | 1.8e-272 | 84.81 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1 | [more] |
| A0A5A7VDG1 | 1.3e-270 | 84.13 | Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G0077... | [more] |
| A0A1S3BDA4 | 1.3e-270 | 84.13 | Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1 | [more] |
