MS006575.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS006575.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	scaffold404: 1481891 .. 1483481 (-)
Sequence length	1005
RNA-Seq Expression	MS006575.1
Synteny	MS006575.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAACGATAAATTTAATCTTTTATAAATATCTTAATATGTTATTTTGTTTGTGTTTGGCAGGTGGTTTGGGTGGCATTGGGGTCAACTATGGGCTGAATGGTGACAATCTACCGCAACCAAACGATGTTATAAGTCTGTATGGGCGGTGTGGTATCGATATCGTGCGGATATTTGAGCCTAATCACGGCGTTTTGGATGCGATGCGCGGGAAACCAAATGCCCTGTGGCTGGGAACAAGAAATGAAGACATACCCAGCTTCGCCACCAACCAAACAGCAGCCAACGCTTGGGTTAACGCTAACGTGGTTCCTTATTATGCAGATGTCAATATCGCATACATCACCGTCGGTAACGAAGTCATCCCTGCCGACCCAGCCGCACCTTTTGTTGCTAATGCCATTAGAAATATAATGCAGGCGCTTGCCATTACCAGAGTCAAGAAGGCCATAAAGGTAATGTGTAAGCTAAGTTTTTTTTTTTTCTTTTTTTTTGTTTAAATGTGGTGTCACCGACATTTAGGTTTAGTTTCACTCAAATTTCTATAATTTCAAAATTTTAATTTTGTTTAGATGGTTAGATAGTTATTATTATTATATTGAACATGTAACATGTTTATTATTTATTTAGCAATGAATTAATGTTTTCTATCGAAGTGAGCATAACTTAACGGTAATTGATATATATCTTCAACCAAGAAGTTGTGAGTTCGATCCTCCACCCCACATGTTGTGCTAAAAAAAATTAATGTTTTCTATTTATATATTAGTTAACACAATATTAGTTGAGTGATAAATAAAAAAATAAGGAAAATAAATAGATAGGTATCGTAAAAGTGACCATTTATGGATAAATCTGTGAGATTTAACCTAAACATAAATATATTAGATTAAAACTTTAAAATTTTATAGATGAAAATATAAATTTTGAAAAGATTAAATTGAAATTAAGCCAAAAGCCACGTAAACCAAGTTTGCAATTTAATCCTAAAAATTATCCAAATTTCTTATTATATATATATATATATACATACTTGCACAGGTAACGACGGTGGTCCCTATGAGCGCCATGGGAGTCTCGTACCCACCCTCAGCTGGAACATTCACAGCTGTCGCCTTGGGGCCTCTAAGCAACATCGCAAAGGTGGTCGATTCTAGCGGCGCACCACTACTATTGAATGTGTATCCATATTTTGCATACGCATCAAACCCACAACAGATCCCTTTAAATTATGCACTCTTCACTTCGACGACGCCTGTTGTGGATGGAAATTTGCACTATTACAATCTGTTTGATGCTATGGTGGATTCTTTCTATGCGGCGTTAGAGAAGATTGGCACTACCAATGTAACACTGGAGATTTCAGAGACTGGATGGCCGACTAAAGGGAATGAGCCGTTCACAAGTGTTGAGAATGCAGTGACATATAACAAAAATTTCGTGAACCATGTGAGCAGTGGAGCAGGAACTCCGAGGAGGCCGAATGTGAAGTATAGTGGAATTTTATTTGAAATGTTCAATGAAGATCTTAAGGCGGCTGGAATTGAGCAGAATTTTGGGTTCTTTTATCCTAATATGCACCCGATTTATCCA

mRNA sequence

ACAACGATAAATTTAATCTTTTATAAATATCTTAATATGTTATTTTGTTTGTGTTTGGCAGGTGGTTTGGGTGGCATTGGGGTCAACTATGGGCTGAATGGTGACAATCTACCGCAACCAAACGATGTTATAAGTCTGTATGGGCGGTGTGGTATCGATATCGTGCGGATATTTGAGCCTAATCACGGCGTTTTGGATGCGATGCGCGGGAAACCAAATGCCCTGTGGCTGGGAACAAGAAATGAAGACATACCCAGCTTCGCCACCAACCAAACAGCAGCCAACGCTTGGGTTAACGCTAACGTGGTTCCTTATTATGCAGATGTCAATATCGCATACATCACCGTCGGTAACGAAGTCATCCCTGCCGACCCAGCCGCACCTTTTGTTGCTAATGCCATTAGAAATATAATGCAGGCGCTTGCCATTACCAGAGTCAAGAAGGCCATAAAGGTAACGACGGTGGTCCCTATGAGCGCCATGGGAGTCTCGTACCCACCCTCAGCTGGAACATTCACAGCTGTCGCCTTGGGGCCTCTAAGCAACATCGCAAAGGTGGTCGATTCTAGCGGCGCACCACTACTATTGAATGTGTATCCATATTTTGCATACGCATCAAACCCACAACAGATCCCTTTAAATTATGCACTCTTCACTTCGACGACGCCTGTTGTGGATGGAAATTTGCACTATTACAATCTGTTTGATGCTATGGTGGATTCTTTCTATGCGGCGTTAGAGAAGATTGGCACTACCAATGTAACACTGGAGATTTCAGAGACTGGATGGCCGACTAAAGGGAATGAGCCGTTCACAAGTGTTGAGAATGCAGTGACATATAACAAAAATTTCGTGAACCATGTGAGCAGTGGAGCAGGAACTCCGAGGAGGCCGAATGTGAAGTATAGTGGAATTTTATTTGAAATGTTCAATGAAGATCTTAAGGCGGCTGGAATTGAGCAGAATTTTGGGTTCTTTTATCCTAATATGCACCCGATTTATCCA

Coding sequence (CDS)

ACAACGATAAATTTAATCTTTTATAAATATCTTAATATGTTATTTTGTTTGTGTTTGGCAGGTGGTTTGGGTGGCATTGGGGTCAACTATGGGCTGAATGGTGACAATCTACCGCAACCAAACGATGTTATAAGTCTGTATGGGCGGTGTGGTATCGATATCGTGCGGATATTTGAGCCTAATCACGGCGTTTTGGATGCGATGCGCGGGAAACCAAATGCCCTGTGGCTGGGAACAAGAAATGAAGACATACCCAGCTTCGCCACCAACCAAACAGCAGCCAACGCTTGGGTTAACGCTAACGTGGTTCCTTATTATGCAGATGTCAATATCGCATACATCACCGTCGGTAACGAAGTCATCCCTGCCGACCCAGCCGCACCTTTTGTTGCTAATGCCATTAGAAATATAATGCAGGCGCTTGCCATTACCAGAGTCAAGAAGGCCATAAAGGTAACGACGGTGGTCCCTATGAGCGCCATGGGAGTCTCGTACCCACCCTCAGCTGGAACATTCACAGCTGTCGCCTTGGGGCCTCTAAGCAACATCGCAAAGGTGGTCGATTCTAGCGGCGCACCACTACTATTGAATGTGTATCCATATTTTGCATACGCATCAAACCCACAACAGATCCCTTTAAATTATGCACTCTTCACTTCGACGACGCCTGTTGTGGATGGAAATTTGCACTATTACAATCTGTTTGATGCTATGGTGGATTCTTTCTATGCGGCGTTAGAGAAGATTGGCACTACCAATGTAACACTGGAGATTTCAGAGACTGGATGGCCGACTAAAGGGAATGAGCCGTTCACAAGTGTTGAGAATGCAGTGACATATAACAAAAATTTCGTGAACCATGTGAGCAGTGGAGCAGGAACTCCGAGGAGGCCGAATGTGAAGTATAGTGGAATTTTATTTGAAATGTTCAATGAAGATCTTAAGGCGGCTGGAATTGAGCAGAATTTTGGGTTCTTTTATCCTAATATGCACCCGATTTATCCA

Protein sequence

TTINLIFYKYLNMLFCLCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVYPYFAYASNPQQIPLNYALFTSTTPVVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNFGFFYPNMHPIYP

Homology

[Close]

BLAST of MS006575.1 vs. NCBI nr
Match: XP_022141986.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-174
Identity = 309/319 (96.87%), Postives = 311/319 (97.49%), Query Frame = 0


Query: 17  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 76
           L L  GLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 80

Query: 77  LGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 136
           LGTRNEDIPSFA NQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN
Sbjct: 81  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 140

Query: 137 IMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLL 196
           IMQALAIT VKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVV SSGAPLLL
Sbjct: 141 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 200

Query: 197 NVYPYFAYASNPQQIPLNYALFTSTTPVVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTL 256
           NVYPYFAYASNPQQI LNYALFTSTTP+VDGNLHYYNLFDAMVDSFYAALEKIG TNVTL
Sbjct: 201 NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL 260

Query: 257 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 316
           EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA
Sbjct: 261 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 320

Query: 317 AGIEQNFGFFYPNMHPIYP 336
           AGIEQNFGFFYPNMHP+YP
Sbjct: 321 AGIEQNFGFFYPNMHPVYP 339

[Close]

BLAST of MS006575.1 vs. NCBI nr
Match: XP_022152302.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 561.6 bits (1446), Expect = 4.5e-156
Identity = 277/320 (86.56%), Postives = 293/320 (91.56%), Query Frame = 0


Query: 17  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 76
           L L  GLGGIGVNYGLNGDNLPQP+DVI+LYGRCGIDIVRIFEPNHGVLDA+ GKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVRIFEPNHGVLDALHGKPNALW 80

Query: 77  LGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 136
           LGTRNEDI SFAT+Q AANAWVNA+V+PYYADVNI YITVGNEV+PADPA PFVANAIRN
Sbjct: 81  LGTRNEDIHSFATDQMAANAWVNAHVLPYYADVNIEYITVGNEVVPADPATPFVANAIRN 140

Query: 137 IMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLL 196
           IMQAL    +KK IKVTTVV M AMGVSYPPSAG FT  A GPLS+IAKVV SSG PLLL
Sbjct: 141 IMQALVTAGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAAAGPLSDIAKVVGSSGTPLLL 200

Query: 197 NVYPYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVT 256
           NVYPYFAYAS+PQQIPLNYALFTSTTP VVDGNL+YYNLFDAMVDSFYAALEKI   NV 
Sbjct: 201 NVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIDAANVR 260

Query: 257 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 316
           LEISETGWPTKGNEPFTSVENAVTYNKNF+NHV+SGAGTPRRPNVKY+GILFEMFNEDLK
Sbjct: 261 LEISETGWPTKGNEPFTSVENAVTYNKNFMNHVASGAGTPRRPNVKYNGILFEMFNEDLK 320

Query: 317 AAGIEQNFGFFYPNMHPIYP 336
           AAG+EQNFGFFYPNM P+YP
Sbjct: 321 AAGVEQNFGFFYPNMQPVYP 340

[Close]

BLAST of MS006575.1 vs. NCBI nr
Match: XP_022135630.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-151
Identity = 270/320 (84.38%), Postives = 290/320 (90.62%), Query Frame = 0


Query: 17  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 76
           L L  GLGGIGVNYGLNG+NLPQ NDVI+LYGRCGIDIVRIFEPNH VLDA+RGKPN LW
Sbjct: 11  LPLICGLGGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLW 70

Query: 77  LGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 136
           LGTRNEDI SFA +Q AANAWVN++VVPYYADVNIAYITVGNEVIP D AAPFVANAIRN
Sbjct: 71  LGTRNEDIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRN 130

Query: 137 IMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLL 196
           IMQAL +T VK+ IKVTTVV MS +GVSYPPS G FT  A GPLS IAKVV SSGAPLLL
Sbjct: 131 IMQALVVTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLL 190

Query: 197 NVYPYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVT 256
           NVYPYFAYASNPQQIPLNYALFTSTTP VVDGNL+YYNLFDAMVDSFYAALEKIG  +V 
Sbjct: 191 NVYPYFAYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVR 250

Query: 257 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 316
           LEISETGWPTKGN+PFTS++NAVTYNKNF+NHV+SGAGTP+RP VKY+GILFEMFNEDLK
Sbjct: 251 LEISETGWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLK 310

Query: 317 AAGIEQNFGFFYPNMHPIYP 336
            AG+EQNFGFFYPNM P+YP
Sbjct: 311 LAGVEQNFGFFYPNMQPVYP 330

[Close]

BLAST of MS006575.1 vs. NCBI nr
Match: XP_038896614.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 497.3 bits (1279), Expect = 1.0e-136
Identity = 244/317 (76.97%), Postives = 275/317 (86.75%), Query Frame = 0


Query: 21  GGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWLGT 80
           GG G +GVNYGLNGDNLP P+DVI+LYGRCGI+I+RIFEPNHGVLDA++G+ N  LWLGT
Sbjct: 29  GGSGTVGVNYGLNGDNLPIPSDVINLYGRCGINIIRIFEPNHGVLDALKGQANLVLWLGT 88

Query: 81  RNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQ 140
           RNEDI  FAT+Q AANAWVNANVVPYY DVNIAYITVGNEV+PAD AAPFVANAI NIMQ
Sbjct: 89  RNEDIEGFATDQVAANAWVNANVVPYYKDVNIAYITVGNEVVPADAAAPFVANAITNIMQ 148

Query: 141 ALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVY 200
           AL    ++  IKVTTVV M+A+ VSYPPSAG FTA A G + +IA V+ SSGAP+L+NVY
Sbjct: 149 ALVNAGIQSDIKVTTVVAMTALEVSYPPSAGAFTATAAGVMKDIANVLGSSGAPILVNVY 208

Query: 201 PYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTLEI 260
           PYFAYASNP+QI LNYALFTS+TP VVDG+LHY+NLFDAMVDSFYAALEKI    V + I
Sbjct: 209 PYFAYASNPEQISLNYALFTSSTPVVVDGDLHYFNLFDAMVDSFYAALEKIDAGGVGIAI 268

Query: 261 SETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAG 320
           SETGWPT GNEPFTSV+NA+TYNKNFV HV+SG GTP+RPNV Y  ILFEMFNEDLKA G
Sbjct: 269 SETGWPTDGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNVFYDAILFEMFNEDLKAPG 328

Query: 321 IEQNFGFFYPNMHPIYP 336
           +EQNFGFFYPNM+P+YP
Sbjct: 329 VEQNFGFFYPNMNPVYP 345

[Close]

BLAST of MS006575.1 vs. NCBI nr
Match: XP_022935980.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata])

HSP 1 Score: 495.4 bits (1274), Expect = 4.0e-136
Identity = 238/319 (74.61%), Postives = 272/319 (85.27%), Query Frame = 0


Query: 19  LAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWL 78
           L  GLG +GVNYGLNGDNLP P++V+ LYGRCGIDI+R++EPNH VLDA++G+ N  +WL
Sbjct: 23  LISGLGAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWL 82

Query: 79  GTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNI 138
           GTRNEDI  FA +Q AAN+WVNANVVPYY  VNIAYITVGNEV+P D AAPFVANAI+NI
Sbjct: 83  GTRNEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNI 142

Query: 139 MQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLN 198
           MQAL I  VK  IKVTTVVPMS + VSYPPSAG F+A A+GPL +I +V+ +SGAPLLLN
Sbjct: 143 MQALVIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLN 202

Query: 199 VYPYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTL 258
            YPYFAYASNPQQI L+YALF ST P VVDGNL Y+NLFDAMVDSFY+ALEKIG   V +
Sbjct: 203 AYPYFAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRI 262

Query: 259 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 318
           +ISETGWP+KGN+PFTSV+NA TYNK FV HV SGAGTP+RPNVKY G+LFEMFNEDLK 
Sbjct: 263 DISETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKT 322

Query: 319 AGIEQNFGFFYPNMHPIYP 336
           AG+EQNFG FYPNMH +YP
Sbjct: 323 AGVEQNFGMFYPNMHSVYP 341

[Close]

BLAST of MS006575.1 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-79
Identity = 152/310 (49.03%), Postives = 202/310 (65.16%), Query Frame = 0


Query: 26  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNEDIP 85
           IGV YG+ G+NLP P++V+SLY    I  +R+++PN   L A+R     + L     D+ 
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 86  SFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAITR 145
           S A+N +AA  W+  NVV Y+  V+  YI VGNE+IP    A ++  A+RNI  AL+   
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 146 VKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVYPYFAYA 205
           ++  IKV+T V    +G SYPPSAG F++ A   LS I + + S+GAPLL+NVYPYF+Y 
Sbjct: 149 LQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSYT 208

Query: 206 SNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALEKIGTTNVTLEISETGWP 265
            NP QI L YALFT++  VV DG   Y NLFDA+VD+ +AALE++G  NV + +SE+GWP
Sbjct: 209 GNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGWP 268

Query: 266 TKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNFG 325
           + G     S  NA TYN+N + HV  G GTPRRP  +    +FEMFNE+ KA GIEQNFG
Sbjct: 269 SAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNFG 328

Query: 326 FFYPNMHPIY 335
            FYPN  P+Y
Sbjct: 329 LFYPNKQPVY 336

[Close]

BLAST of MS006575.1 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.1e-75
Identity = 150/312 (48.08%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 26  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRG-KPNALWLGTRNEDI 85
           IG+NYGL GDNLP P++VI+LY   GI  +RIF+PN  VL+A+RG +   + +G +++D+
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 86  PSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAIT 145
            + A ++ A   W  AN+  Y ADVNI +ITVGNEVIP  P  P V   ++++   +   
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPG-PIGPQVLPVMQSLTNLVKSR 153

Query: 146 RVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVYPYFAY 205
            +   I ++TVV MS +  SYPPSAG FT+ A   L  + K++  +  P+L+N+YPYFAY
Sbjct: 154 NL--PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAY 213

Query: 206 ASNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALEKIGTTNVTLEISETGW 265
           AS+P  I L+YA F + + VV DG+L Y N+FDA+ D+F  A+EK G  N+ + +SETGW
Sbjct: 214 ASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGW 273

Query: 266 PTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNF 325
           P+ GN  FT+   A TYN+NFV H++SG GTP+RPN   +G LF  FNE+ K AG EQNF
Sbjct: 274 PSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNF 333

Query: 326 GFFYP-NMHPIY 335
           G + P +M PIY
Sbjct: 334 GLYNPSDMKPIY 342

[Close]

BLAST of MS006575.1 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 145/328 (44.21%), Postives = 209/328 (63.72%), Query Frame = 0


Query: 10  YLNMLFCLCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMR 69
           ++N    +        IG+NYGL GDNLP P+ VI+LY    I  +RIF+PN  VL+A+R
Sbjct: 27  FVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALR 86

Query: 70  G-KPNALWLGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAP 129
           G +  A+ +G R++D+ + + ++ A   W   N+ PY +D+NIA+ITVGNEVIP  P  P
Sbjct: 87  GHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPG-PIGP 146

Query: 130 FVANAIRNIMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVD 189
            V   ++++   +    +   I ++TVV M  +  SYPPSAG FT+ A   L  + K++ 
Sbjct: 147 QVLPVMQSLTNLVKSRNL--PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLS 206

Query: 190 SSGAPLLLNVYPYFAYASNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALE 249
            + +P+L+ +YPYF+YAS+P  I L+YA F +   VV DG+L Y N+FDA+ D+F  A+E
Sbjct: 207 QTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAME 266

Query: 250 KIGTTNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILF 309
           K G  ++ + +SETGWP+ GN   T+ + A TYN+NFV H++SG GTP+RPN    G LF
Sbjct: 267 KEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLF 326

Query: 310 EMFNEDLKAAGIEQNFGFFYPN-MHPIY 335
             FNE+ K  G EQNFG + PN M PIY
Sbjct: 327 ATFNENQKPVGTEQNFGLYNPNDMKPIY 351

[Close]

BLAST of MS006575.1 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-66
Identity = 142/316 (44.94%), Postives = 196/316 (62.03%), Query Frame = 0


Query: 22  GLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRN 81
           G+ GIGVNYG+ G +LP P+ V++LY    I  VRIF P+  VL+A+R     + LGT N
Sbjct: 3   GVEGIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALRNSGLGVVLGTLN 62

Query: 82  EDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQAL 141
            D+   A++ + A +WV++ V P+   V+  YI  GNEVIP + AA  V  A++N+  AL
Sbjct: 63  SDLAPLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEAAL 122

Query: 142 AITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVYPY 201
               +  ++ VTT +  S +G SYPPS GTF+  AL  +  I   + SSG PLL+NVYPY
Sbjct: 123 QAAGL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVYPY 182

Query: 202 FAYASNPQQIPLNYALFTST--TPVVDGNLHYYNLFDAMVDSFYAALEKI-GTTNVTLEI 261
           FAY+++P  + L+YAL +S+    V D  + Y N+FDA++D+ YAA+EK  G  ++ L +
Sbjct: 183 FAYSADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLELVV 242

Query: 262 SETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAG 321
           SETGWP+ G     SVENA  Y  N V HV    GTPRRP       +F MFNE+ K  G
Sbjct: 243 SETGWPSGGGGYGASVENAAAYINNLVRHV---GGTPRRPGKAVETYIFAMFNENQKPEG 302

Query: 322 IEQNFGFFYPNMHPIY 335
           +EQNFG F P+M  +Y
Sbjct: 303 VEQNFGMFQPDMSQVY 312

[Close]

BLAST of MS006575.1 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.0e-62
Identity = 136/310 (43.87%), Postives = 192/310 (61.94%), Query Frame = 0


Query: 26  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNEDIP 85
           IGV  G+ GD+LP   +V++LY    I  +R+++PN   L+A+RG    L LG  NE++ 
Sbjct: 40  IGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVPNENLQ 99

Query: 86  SFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAITR 145
             A +Q  ANAWV  N V  YA+V   YI VGNEV P+D  A F+  A+RNI +A+++  
Sbjct: 100 YIALSQANANAWVQNN-VRNYANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAISLAG 159

Query: 146 VKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVYPYFAYA 205
           + K IKV+T +    +G ++PPS G+F +     L  I + + S  +PLL+N+YPYFAY+
Sbjct: 160 LAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPYFAYS 219

Query: 206 SNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALEKIGTTNVTLEISETGWP 265
            N Q I L+YALFT+ + VV DGN  Y NLFDAM+D  YAALEK G  ++ + ISETGWP
Sbjct: 220 GNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISETGWP 279

Query: 266 TKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNFG 325
           +      T+++NA T+  N + HV    GTPRRP       +F MF+E+ K   +E+++G
Sbjct: 280 SAAGTA-TTIDNARTFISNLIQHVKE--GTPRRPGRPIETYIFAMFDENRKTPELEKHWG 339

Query: 326 FFYPNMHPIY 335
            F P   P Y
Sbjct: 340 LFSPTKQPKY 345

[Close]

BLAST of MS006575.1 vs. ExPASy TrEMBL
Match: A0A6J1CKU8 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.1e-175
Identity = 309/319 (96.87%), Postives = 311/319 (97.49%), Query Frame = 0


Query: 17  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 76
           L L  GLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 80

Query: 77  LGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 136
           LGTRNEDIPSFA NQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN
Sbjct: 81  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 140

Query: 137 IMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLL 196
           IMQALAIT VKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVV SSGAPLLL
Sbjct: 141 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 200

Query: 197 NVYPYFAYASNPQQIPLNYALFTSTTPVVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTL 256
           NVYPYFAYASNPQQI LNYALFTSTTP+VDGNLHYYNLFDAMVDSFYAALEKIG TNVTL
Sbjct: 201 NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL 260

Query: 257 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 316
           EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA
Sbjct: 261 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 320

Query: 317 AGIEQNFGFFYPNMHPIYP 336
           AGIEQNFGFFYPNMHP+YP
Sbjct: 321 AGIEQNFGFFYPNMHPVYP 339

[Close]

BLAST of MS006575.1 vs. ExPASy TrEMBL
Match: A0A6J1DFU0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.2e-156
Identity = 277/320 (86.56%), Postives = 293/320 (91.56%), Query Frame = 0


Query: 17  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 76
           L L  GLGGIGVNYGLNGDNLPQP+DVI+LYGRCGIDIVRIFEPNHGVLDA+ GKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVRIFEPNHGVLDALHGKPNALW 80

Query: 77  LGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 136
           LGTRNEDI SFAT+Q AANAWVNA+V+PYYADVNI YITVGNEV+PADPA PFVANAIRN
Sbjct: 81  LGTRNEDIHSFATDQMAANAWVNAHVLPYYADVNIEYITVGNEVVPADPATPFVANAIRN 140

Query: 137 IMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLL 196
           IMQAL    +KK IKVTTVV M AMGVSYPPSAG FT  A GPLS+IAKVV SSG PLLL
Sbjct: 141 IMQALVTAGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAAAGPLSDIAKVVGSSGTPLLL 200

Query: 197 NVYPYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVT 256
           NVYPYFAYAS+PQQIPLNYALFTSTTP VVDGNL+YYNLFDAMVDSFYAALEKI   NV 
Sbjct: 201 NVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIDAANVR 260

Query: 257 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 316
           LEISETGWPTKGNEPFTSVENAVTYNKNF+NHV+SGAGTPRRPNVKY+GILFEMFNEDLK
Sbjct: 261 LEISETGWPTKGNEPFTSVENAVTYNKNFMNHVASGAGTPRRPNVKYNGILFEMFNEDLK 320

Query: 317 AAGIEQNFGFFYPNMHPIYP 336
           AAG+EQNFGFFYPNM P+YP
Sbjct: 321 AAGVEQNFGFFYPNMQPVYP 340

[Close]

BLAST of MS006575.1 vs. ExPASy TrEMBL
Match: A0A6J1C1J7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.3e-152
Identity = 270/320 (84.38%), Postives = 290/320 (90.62%), Query Frame = 0


Query: 17  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 76
           L L  GLGGIGVNYGLNG+NLPQ NDVI+LYGRCGIDIVRIFEPNH VLDA+RGKPN LW
Sbjct: 11  LPLICGLGGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLW 70

Query: 77  LGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 136
           LGTRNEDI SFA +Q AANAWVN++VVPYYADVNIAYITVGNEVIP D AAPFVANAIRN
Sbjct: 71  LGTRNEDIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRN 130

Query: 137 IMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLL 196
           IMQAL +T VK+ IKVTTVV MS +GVSYPPS G FT  A GPLS IAKVV SSGAPLLL
Sbjct: 131 IMQALVVTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLL 190

Query: 197 NVYPYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVT 256
           NVYPYFAYASNPQQIPLNYALFTSTTP VVDGNL+YYNLFDAMVDSFYAALEKIG  +V 
Sbjct: 191 NVYPYFAYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVR 250

Query: 257 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 316
           LEISETGWPTKGN+PFTS++NAVTYNKNF+NHV+SGAGTP+RP VKY+GILFEMFNEDLK
Sbjct: 251 LEISETGWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLK 310

Query: 317 AAGIEQNFGFFYPNMHPIYP 336
            AG+EQNFGFFYPNM P+YP
Sbjct: 311 LAGVEQNFGFFYPNMQPVYP 330

[Close]

BLAST of MS006575.1 vs. ExPASy TrEMBL
Match: A0A6J1FC84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.9e-136
Identity = 238/319 (74.61%), Postives = 272/319 (85.27%), Query Frame = 0


Query: 19  LAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWL 78
           L  GLG +GVNYGLNGDNLP P++V+ LYGRCGIDI+R++EPNH VLDA++G+ N  +WL
Sbjct: 23  LISGLGAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWL 82

Query: 79  GTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNI 138
           GTRNEDI  FA +Q AAN+WVNANVVPYY  VNIAYITVGNEV+P D AAPFVANAI+NI
Sbjct: 83  GTRNEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNI 142

Query: 139 MQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLN 198
           MQAL I  VK  IKVTTVVPMS + VSYPPSAG F+A A+GPL +I +V+ +SGAPLLLN
Sbjct: 143 MQALVIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLN 202

Query: 199 VYPYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTL 258
            YPYFAYASNPQQI L+YALF ST P VVDGNL Y+NLFDAMVDSFY+ALEKIG   V +
Sbjct: 203 AYPYFAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRI 262

Query: 259 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 318
           +ISETGWP+KGN+PFTSV+NA TYNK FV HV SGAGTP+RPNVKY G+LFEMFNEDLK 
Sbjct: 263 DISETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKT 322

Query: 319 AGIEQNFGFFYPNMHPIYP 336
           AG+EQNFG FYPNMH +YP
Sbjct: 323 AGVEQNFGMFYPNMHSVYP 341

[Close]

BLAST of MS006575.1 vs. ExPASy TrEMBL
Match: A0A6J1IM63 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111476518 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.8e-135
Identity = 236/319 (73.98%), Postives = 272/319 (85.27%), Query Frame = 0


Query: 19  LAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWL 78
           L  GLG +GVNYGLNGDNLP P++V+ LYGRCGIDI+R++EPNH VLDA++G+ N  +WL
Sbjct: 23  LISGLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVYEPNHSVLDALKGRRNLVVWL 82

Query: 79  GTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNI 138
           GTRNEDI  FA +Q AAN+WVNANVVPYY  VNIAYITVGNEV+P D AAPFVANAI+NI
Sbjct: 83  GTRNEDIKGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNI 142

Query: 139 MQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLN 198
           MQAL I  VK  IKV+TVVPMSA+ VS+PPSAG F+  A+GPL +I  V+ +SGAPLLLN
Sbjct: 143 MQALVIAGVKSDIKVSTVVPMSALAVSHPPSAGAFSTTAIGPLKDITLVLGTSGAPLLLN 202

Query: 199 VYPYFAYASNPQQIPLNYALFTSTTP-VVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTL 258
            Y YFAYASNPQQI L+YALFTST P VVDGNL Y+NLFDAMVDSFY+ALEKIG   V +
Sbjct: 203 AYTYFAYASNPQQISLDYALFTSTAPVVVDGNLQYFNLFDAMVDSFYSALEKIGAEGVRI 262

Query: 259 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 318
           +ISETGWP+KGN+PFTSV+NA TYNK FV HV SGAGTP+RPNVKY G+LFEMFNE+LK 
Sbjct: 263 DISETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNENLKT 322

Query: 319 AGIEQNFGFFYPNMHPIYP 336
           AG+EQNFG FYPNMHP+YP
Sbjct: 323 AGVEQNFGMFYPNMHPVYP 341

[Close]

BLAST of MS006575.1 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 282.0 bits (720), Expect = 6.5e-76
Identity = 150/312 (48.08%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 26  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRG-KPNALWLGTRNEDI 85
           IG+NYGL GDNLP P++VI+LY   GI  +RIF+PN  VL+A+RG +   + +G +++D+
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 86  PSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAIT 145
            + A ++ A   W  AN+  Y ADVNI +ITVGNEVIP  P  P V   ++++   +   
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPG-PIGPQVLPVMQSLTNLVKSR 153

Query: 146 RVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVYPYFAY 205
            +   I ++TVV MS +  SYPPSAG FT+ A   L  + K++  +  P+L+N+YPYFAY
Sbjct: 154 NL--PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAY 213

Query: 206 ASNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALEKIGTTNVTLEISETGW 265
           AS+P  I L+YA F + + VV DG+L Y N+FDA+ D+F  A+EK G  N+ + +SETGW
Sbjct: 214 ASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGW 273

Query: 266 PTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNF 325
           P+ GN  FT+   A TYN+NFV H++SG GTP+RPN   +G LF  FNE+ K AG EQNF
Sbjct: 274 PSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNF 333

Query: 326 GFFYP-NMHPIY 335
           G + P +M PIY
Sbjct: 334 GLYNPSDMKPIY 342

[Close]

BLAST of MS006575.1 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 145/328 (44.21%), Postives = 209/328 (63.72%), Query Frame = 0


Query: 10  YLNMLFCLCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMR 69
           ++N    +        IG+NYGL GDNLP P+ VI+LY    I  +RIF+PN  VL+A+R
Sbjct: 27  FVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALR 86

Query: 70  G-KPNALWLGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAP 129
           G +  A+ +G R++D+ + + ++ A   W   N+ PY +D+NIA+ITVGNEVIP  P  P
Sbjct: 87  GHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPG-PIGP 146

Query: 130 FVANAIRNIMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVD 189
            V   ++++   +    +   I ++TVV M  +  SYPPSAG FT+ A   L  + K++ 
Sbjct: 147 QVLPVMQSLTNLVKSRNL--PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLS 206

Query: 190 SSGAPLLLNVYPYFAYASNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALE 249
            + +P+L+ +YPYF+YAS+P  I L+YA F +   VV DG+L Y N+FDA+ D+F  A+E
Sbjct: 207 QTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAME 266

Query: 250 KIGTTNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILF 309
           K G  ++ + +SETGWP+ GN   T+ + A TYN+NFV H++SG GTP+RPN    G LF
Sbjct: 267 KEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLF 326

Query: 310 EMFNEDLKAAGIEQNFGFFYPN-MHPIY 335
             FNE+ K  G EQNFG + PN M PIY
Sbjct: 327 ATFNENQKPVGTEQNFGLYNPNDMKPIY 351

[Close]

BLAST of MS006575.1 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 146/320 (45.62%), Postives = 200/320 (62.50%), Query Frame = 0


Query: 17  LCLAGGLGGI-GVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNAL 76
           L  AG +    GV YG NGDNLP P   +SLY +  +  +R++EP   ++ +++G    +
Sbjct: 14  LTTAGNMNSFAGVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQGTGLLV 73

Query: 77  WLGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIR 136
            +G RNE I + A     A  W    + P Y +V   +ITVGNEVI  +    +V  A++
Sbjct: 74  AIGPRNEAIKTLAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGE-IGRYVPQAMK 133

Query: 137 NIMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLL 196
           NI  AL      K I VTTV+  +A+  SYPPSAG F       ++ I  ++ S+ +PL+
Sbjct: 134 NIKAALTEIGNSK-IHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLM 193

Query: 197 LNVYPYFAYASNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALEKIGTTNV 256
           +NVYPYFAYAS+P  + L YA F ST+PVV DG   Y N+FDA +D+F  ALEKI   +V
Sbjct: 194 VNVYPYFAYASDPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSV 253

Query: 257 TLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDL 316
            + ++ETGWPT+GN+P+TSVENA  YN+  +  +++G GTPRRPNV      FEMFNEDL
Sbjct: 254 KVYVAETGWPTRGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDL 313

Query: 317 KAAGIEQNFGFFYPNMHPIY 335
           K   +EQ+FGFF PNM P+Y
Sbjct: 314 KQGAVEQSFGFFDPNMAPVY 330

[Close]

BLAST of MS006575.1 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 151/321 (47.04%), Postives = 200/321 (62.31%), Query Frame = 0


Query: 18  CLAGGLGGI-GVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 77
           C AG  G I G  YG NG+NLP P D ++LY    ID +R++EP   +L+A+RG    + 
Sbjct: 21  CTAGVAGDITGDCYGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADMLEALRGSGLLVA 80

Query: 78  LGTRNEDIPSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 137
            G RNEDI S A +  AA  +V+  + PY  DV I +IT+GNEV P +  A FVA AI+N
Sbjct: 81  FGPRNEDIQSLAHDPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGE-IAQFVAAAIKN 140

Query: 138 IMQALAITRVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLL 197
           +  AL  + V   I VTTV+ M+A+  +YPPSA TF       ++ I  ++  + +PL+ 
Sbjct: 141 VNVALTNSGV-TGISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITSILSETNSPLMT 200

Query: 198 NVYPYFAYASNPQQIPLNYALFTSTTPVV-DGNLHYYNLFDAMVDSFYAALEKIGTTNVT 257
           N+YPYFAYAS+P  I L+YA F S TPVV DG+L+Y N+F+AMVD F AALEKI   NV 
Sbjct: 201 NIYPYFAYASDPYHISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNAALEKINAANVV 260

Query: 258 LEISETGWPTKGNEPFTSVENAVTYNKNF--VNHVSSGAGTPRRPNVKYSGILFEMFNED 317
           + ++ETGWPT+GN P TSV+NA  YN         +    TPRR N      LF MF E+
Sbjct: 261 VMVAETGWPTEGNPPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPVDVFLFAMFKEN 320

Query: 318 LKAAGIEQNFGFFYPNMHPIY 335
            K   +EQ+FG F P+M P+Y
Sbjct: 321 QKDGPVEQSFGIFAPDMTPVY 339

[Close]

BLAST of MS006575.1 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 6.3e-71
Identity = 143/311 (45.98%), Postives = 198/311 (63.67%), Query Frame = 0


Query: 26  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWLGTRNEDI 85
           IG+NYGL GDN P P++V++LY   GI  +RIF+P   VL+A+RG  N  + +G R++D+
Sbjct: 34  IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQDL 93

Query: 86  PSFATNQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAIT 145
            + + N+ A   W   N+ PY ADVNIA+ITVGNEVIP  P  P V   ++++   + + 
Sbjct: 94  GALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPG-PIGPQVLPVMQSL--TILVK 153

Query: 146 RVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVDSSGAPLLLNVYPYFAY 205
            +   I ++TVV MS +  SYPPSAG FT+ A   L  + K++  +  P+L+N+YPYF Y
Sbjct: 154 SMNLPISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYFPY 213

Query: 206 ASNPQQIPLNYALF-TSTTPVVDGNLHYYNLFDAMVDSFYAALEKIGTTNVTLEISETGW 265
           AS+P  IPL+YA F T  T V DG L Y N+FDA+ D+F  A+EK G  ++ + +SETGW
Sbjct: 214 ASDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGW 273

Query: 266 PTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNF 325
           P+ GN   T+ + A TYN+NFV H+ SG GTP+RPN    G LF  FNE+ K AG +   
Sbjct: 274 PSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPAGTD--- 332

Query: 326 GFFYPNMHPIY 335
                 M PIY
Sbjct: 334 ------MKPIY 332

The following BLAST results are available for this feature:

BLAST of MS006575.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141986.1	1.3e-174	96.87	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022152302.1	4.5e-156	86.56	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022135630.1	1.5e-151	84.38	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_038896614.1	1.0e-136	76.97	glucan endo-1,3-beta-glucosidase-like [Benincasa hispida]	[more]
XP_022935980.1	4.0e-136	74.61	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata]	[more]

BLAST of MS006575.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22317	1.2e-79	49.03	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
O49352	9.1e-75	48.08	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49353	1.6e-71	44.21	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q02439	1.2e-66	44.94	Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4...	[more]
P52408	1.0e-62	43.87	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]

BLAST of MS006575.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKU8	6.1e-175	96.87	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 ...	[more]
A0A6J1DFU0	2.2e-156	86.56	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 ...	[more]
A0A6J1C1J7	7.3e-152	84.38	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 ...	[more]
A0A6J1FC84	1.9e-136	74.61	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 P...	[more]
A0A6J1IM63	2.8e-135	73.98	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111476518 PE=...	[more]

BLAST of MS006575.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20330.1	6.5e-76	48.08	beta-1,3-glucanase 4	[more]
AT5G20340.1	1.1e-72	44.21	beta-1,3-glucanase 5	[more]
AT1G77780.1	1.3e-71	45.63	Glycosyl hydrolase superfamily protein	[more]
AT1G77790.1	1.6e-71	47.04	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	6.3e-71	45.98	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 26..334 e-value: 1.5E-88 score: 297.4
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..335 e-value: 5.9E-111 score: 372.5
None	No IPR available	PANTHER	PTHR32227:SF248	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 17..334
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 17..334
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..335

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS006575	MS006575	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS006575.1-cds	MS006575.1-cds-scaffold404:1481891..1482442	CDS
MS006575.1-cds	MS006575.1-cds-scaffold404:1483029..1483481	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS006575.1	MS006575.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds