MS001254 (gene) Bitter gourd (TR) v1

Overview
NameMS001254
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING/U-box superfamily protein
Locationscaffold36: 2683020 .. 2686036 (+)
RNA-Seq ExpressionMS001254
SyntenyMS001254
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAATATCCTAGTAAAAGAGCTGCTGATGGATACGTATCCAGAAGGGGACCCGGTACGATTTTGAGAGATACTGCAAATAACAGAGACCAAGATGGTAAATATTGTAGCCGAATTGGTTGCAGTGGCAGACTAAACTCCCCAAAGGGCACTCGGAGTAGTTATTCAGAGAAACTTAAATCTCCATTGCAGTCTTTTCGAACCAATTCAAGTGGCAAAGAAGTTGTCGGAAGTTCCTCTAGGACTTACTATGCTGTTCGCGGTTCCAAGAAATCTGTAGCTGAAACTCAGAGAAAACTGTCCACCCAGTTAGAAACAGACTCTTCAGAAACTAGTAGCACCCAGGATGAGCCAGAGATTTCACAAGTTGTCCCGTCAAATGAAAGGATACGAACAGGGCTTCATGTCGGTTTTAAAAGTTCCGACTCTGCAGATGACACAATGGAACCAGGAAGCTCCAACGCTGCATCTAACACTAGAGGGCGAAGGGATTTCAATCGGAGATCTGGAACGCGTAGTAAGGATACTGCAGCAGGTTCTTCTGTGGTGACGGGGCCTAAAAGTTCTCGCCAGACGTCACGTGGTGGTGCAGGTAGGCATACCTTGAGAAATTTCAGATGTAATTCAATATCTGATGTCATCTCATCAGGTTGTTCTTCATCAGATCCAAATCTTAGTAAACGGAAGGATACAGCCAAAAAAAGGAATTCTGAAGCAGAAAGTAGCTCTACGGGAGGAAAGAAGATGAATGGATCATCCTCAGAAAGAAGGATTACGAGCTCTGGTTATGGCGTTTCAATATCTGATTCAAGAGGAGCCAAAAATGGAACTTCTCCCAGGGAAAATGGCATTGCAGGTCGATCTAGGGCATCAATCAACGGGATGTCAAGAGCAAGATCGCATGGCAACAGACCCGACAGAAACAGTACATCGTTGCAAGAGTCACGGTCCATGATCTCTCAGGTGCCTCATGTTCATCAACCCGACAGCTCAATAGATAGTAATGCGCATGAAGTCTCTACAGATTTGATTTCAGATCACCCAATTTCTTACAGAAGATCAGATAGCATCAATGAGAATATACTTGGTAATAGACCTGCTAGTCCTGCAGAAATTGGATTGGCACGCACTTTAGCAACTCGAGATAGCTTTCGACACTATGGTATTGCAGAGGTACATTTCCATATCATAATTCCATCTATTTTGTGAAGATAAATCGAAAGAACACTTCATTTTCTCAGTCTAGTTAATTTTAAAAAATTTGCATCTGCTTTTTTAAGGTATTGTTGGCTCTTGAAAGGATTGAACATGAAGAAGAGCTAACTTATGAGGTAGGTAGTTCCTTGGTTTTATCTTTATTAATCTAGGGTTAATTTACAAATTTAGTCCTTGAGCTTCGTGAGTTGTCCTTTTATTTTAACAGGTTCTTGAACCTTTTCAATTGTGCTTAACAGGATTTTGAATTTTGAATTTCGTATCAAATAGGCCTAAAATGAAAGTTGAGGGACCTATTAGACACAATATTTAAAGTTTAGAGAATTCTTAGCACCTTTTAAAATTTATTTAGAAACCTATTAGAAACAACTTGAAATTTCAAGGACCAAACTTTGTTATTTAACCATAAGTTATTAAGTCACTACATGTTGAGCTAACATTTAATTTGCCCTGCAGCAAGTAATTCTTTTAGAGACCAACCTTTTCCTTAGTGGCTTAAACTTCTATGATCAGCATAGAGACATGAGGCTGGATATTGATAACATGTCATATGAGGTTTGATATCTTCTTTGGAAATACTGTGCTCCCTTTATATCTAATATATCCAAATGTACATCCTCTGTCCTGCCTTTTTGATGTTATTGTGGACCATTTCTTTTGTTACCCAAACAATTCATTCAATTGTCCTTGGGGGAGTTGCATTTTAGCATCGACTATTACACTCCTCCCCATATGCTGTGTCGGTTGTTCTCGTATTTTTACTTATCTACTATGTTGTGCTTATGACAGGAATTACTAGCTCTAGAAGAGAGAATGGGAAGCGTGAGTACAGCGGTTACCGAGGAAGCATTGTCACAGTGCTTGAATAGAAGCACATATCAGTCTAGACATGCAGAGAATACATCTGCAGGCAGTAGCGAGGGCAGCAGCGAGTACGAGGATGGCGTCAAATGTTGTATATGCCAGGTCTGTTTATACCAGCTGTTTGTTCTGAACCTCTTGTTATACTATAAAGTTAGAATCCAAAAGGGTAAAACAGCCACTAATCAGCCATTATTTATTATAAGCTTAGTTTTGAGAATGGACTTGATGAGGCTGCTAAAAGACCTGAACTCAAGGAACTGATTTGTTATCCTGTTGTATTATAGGTTACCATAGTGTCAGGATTTGCATGCATTTACCAACATCTCTGGCTATTAGTTACCAGACATCTTCGATTTAATTGAATTTTGGTCGTAATATCTTTCTGGGGCCTAAATGTATACTCGTTTTCTTTTCAGTCCGTGAACTTTTGAACATCACTTTTGGTAAAACTTTACTCGTTTTTCGTCTTTCTACATGTCAATTTTAATCCTTCAGCTTTCATCAAACTCTAGTTTTGGTCGTTACCATAAAAACTTTTTTGAGTTTACCACATTACACAATTTATTATCTATGTATTAAATCAAGGTATCACTTTTATGGGAAAGATTAGAGACGTAGTTTAAGAGAAGATGAAGGAATAGGAACCAAAATAGAAAATGACAAAAAATTCAAGTACTATAACCATATTTAAACTACCTGAGTTTCTTGATCATTCAAATATTCGATCTGAGATCCGCACCTCAATCAGTTTAAGTAACAAGGGGTTTGACTTTTTTTCCTTGTTGCAGGAAGAATACTCGAATGGAGATGAAGTCGGGACGCTGCAGTGTGAGCATACTTACCATGAAGGGTGCATACAACAGTGGCTGCGGCTGAAGAACTGGTGCCCCATTTGCAAAGCATCTGTGGAGGAACCTGCTTCAGCTTTGCCTTCA

mRNA sequence

ATGGAAGAATATCCTAGTAAAAGAGCTGCTGATGGATACGTATCCAGAAGGGGACCCGGTACGATTTTGAGAGATACTGCAAATAACAGAGACCAAGATGGTAAATATTGTAGCCGAATTGGTTGCAGTGGCAGACTAAACTCCCCAAAGGGCACTCGGAGTAGTTATTCAGAGAAACTTAAATCTCCATTGCAGTCTTTTCGAACCAATTCAAGTGGCAAAGAAGTTGTCGGAAGTTCCTCTAGGACTTACTATGCTGTTCGCGGTTCCAAGAAATCTGTAGCTGAAACTCAGAGAAAACTGTCCACCCAGTTAGAAACAGACTCTTCAGAAACTAGTAGCACCCAGGATGAGCCAGAGATTTCACAAGTTGTCCCGTCAAATGAAAGGATACGAACAGGGCTTCATGTCGGTTTTAAAAGTTCCGACTCTGCAGATGACACAATGGAACCAGGAAGCTCCAACGCTGCATCTAACACTAGAGGGCGAAGGGATTTCAATCGGAGATCTGGAACGCGTAGTAAGGATACTGCAGCAGGTTCTTCTGTGGTGACGGGGCCTAAAAGTTCTCGCCAGACGTCACGTGGTGGTGCAGGTAGGCATACCTTGAGAAATTTCAGATGTAATTCAATATCTGATGTCATCTCATCAGGTTGTTCTTCATCAGATCCAAATCTTAGTAAACGGAAGGATACAGCCAAAAAAAGGAATTCTGAAGCAGAAAGTAGCTCTACGGGAGGAAAGAAGATGAATGGATCATCCTCAGAAAGAAGGATTACGAGCTCTGGTTATGGCGTTTCAATATCTGATTCAAGAGGAGCCAAAAATGGAACTTCTCCCAGGGAAAATGGCATTGCAGGTCGATCTAGGGCATCAATCAACGGGATGTCAAGAGCAAGATCGCATGGCAACAGACCCGACAGAAACAGTACATCGTTGCAAGAGTCACGGTCCATGATCTCTCAGGTGCCTCATGTTCATCAACCCGACAGCTCAATAGATAGTAATGCGCATGAAGTCTCTACAGATTTGATTTCAGATCACCCAATTTCTTACAGAAGATCAGATAGCATCAATGAGAATATACTTGGTAATAGACCTGCTAGTCCTGCAGAAATTGGATTGGCACGCACTTTAGCAACTCGAGATAGCTTTCGACACTATGGTATTGCAGAGGTATTGTTGGCTCTTGAAAGGATTGAACATGAAGAAGAGCTAACTTATGAGCAAGTAATTCTTTTAGAGACCAACCTTTTCCTTAGTGGCTTAAACTTCTATGATCAGCATAGAGACATGAGGCTGGATATTGATAACATGTCATATGAGGAATTACTAGCTCTAGAAGAGAGAATGGGAAGCGTGAGTACAGCGGTTACCGAGGAAGCATTGTCACAGTGCTTGAATAGAAGCACATATCAGTCTAGACATGCAGAGAATACATCTGCAGGCAGTAGCGAGGGCAGCAGCGAGTACGAGGATGGCGTCAAATGTTGTATATGCCAGGAAGAATACTCGAATGGAGATGAAGTCGGGACGCTGCAGTGTGAGCATACTTACCATGAAGGGTGCATACAACAGTGGCTGCGGCTGAAGAACTGGTGCCCCATTTGCAAAGCATCTGTGGAGGAACCTGCTTCAGCTTTGCCTTCA

Coding sequence (CDS)

ATGGAAGAATATCCTAGTAAAAGAGCTGCTGATGGATACGTATCCAGAAGGGGACCCGGTACGATTTTGAGAGATACTGCAAATAACAGAGACCAAGATGGTAAATATTGTAGCCGAATTGGTTGCAGTGGCAGACTAAACTCCCCAAAGGGCACTCGGAGTAGTTATTCAGAGAAACTTAAATCTCCATTGCAGTCTTTTCGAACCAATTCAAGTGGCAAAGAAGTTGTCGGAAGTTCCTCTAGGACTTACTATGCTGTTCGCGGTTCCAAGAAATCTGTAGCTGAAACTCAGAGAAAACTGTCCACCCAGTTAGAAACAGACTCTTCAGAAACTAGTAGCACCCAGGATGAGCCAGAGATTTCACAAGTTGTCCCGTCAAATGAAAGGATACGAACAGGGCTTCATGTCGGTTTTAAAAGTTCCGACTCTGCAGATGACACAATGGAACCAGGAAGCTCCAACGCTGCATCTAACACTAGAGGGCGAAGGGATTTCAATCGGAGATCTGGAACGCGTAGTAAGGATACTGCAGCAGGTTCTTCTGTGGTGACGGGGCCTAAAAGTTCTCGCCAGACGTCACGTGGTGGTGCAGGTAGGCATACCTTGAGAAATTTCAGATGTAATTCAATATCTGATGTCATCTCATCAGGTTGTTCTTCATCAGATCCAAATCTTAGTAAACGGAAGGATACAGCCAAAAAAAGGAATTCTGAAGCAGAAAGTAGCTCTACGGGAGGAAAGAAGATGAATGGATCATCCTCAGAAAGAAGGATTACGAGCTCTGGTTATGGCGTTTCAATATCTGATTCAAGAGGAGCCAAAAATGGAACTTCTCCCAGGGAAAATGGCATTGCAGGTCGATCTAGGGCATCAATCAACGGGATGTCAAGAGCAAGATCGCATGGCAACAGACCCGACAGAAACAGTACATCGTTGCAAGAGTCACGGTCCATGATCTCTCAGGTGCCTCATGTTCATCAACCCGACAGCTCAATAGATAGTAATGCGCATGAAGTCTCTACAGATTTGATTTCAGATCACCCAATTTCTTACAGAAGATCAGATAGCATCAATGAGAATATACTTGGTAATAGACCTGCTAGTCCTGCAGAAATTGGATTGGCACGCACTTTAGCAACTCGAGATAGCTTTCGACACTATGGTATTGCAGAGGTATTGTTGGCTCTTGAAAGGATTGAACATGAAGAAGAGCTAACTTATGAGCAAGTAATTCTTTTAGAGACCAACCTTTTCCTTAGTGGCTTAAACTTCTATGATCAGCATAGAGACATGAGGCTGGATATTGATAACATGTCATATGAGGAATTACTAGCTCTAGAAGAGAGAATGGGAAGCGTGAGTACAGCGGTTACCGAGGAAGCATTGTCACAGTGCTTGAATAGAAGCACATATCAGTCTAGACATGCAGAGAATACATCTGCAGGCAGTAGCGAGGGCAGCAGCGAGTACGAGGATGGCGTCAAATGTTGTATATGCCAGGAAGAATACTCGAATGGAGATGAAGTCGGGACGCTGCAGTGTGAGCATACTTACCATGAAGGGTGCATACAACAGTGGCTGCGGCTGAAGAACTGGTGCCCCATTTGCAAAGCATCTGTGGAGGAACCTGCTTCAGCTTTGCCTTCA

Protein sequence

MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKLKSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPEISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAGSSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEAESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRARSHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINENILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFLSGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKASVEEPASALPS
Homology
BLAST of MS001254 vs. NCBI nr
Match: XP_022131770.1 (dentin sialophosphoprotein-like isoform X1 [Momordica charantia] >XP_022131771.1 dentin sialophosphoprotein-like isoform X1 [Momordica charantia] >XP_022131772.1 dentin sialophosphoprotein-like isoform X1 [Momordica charantia])

HSP 1 Score: 1036.6 bits (2679), Expect = 7.9e-299
Identity = 548/550 (99.64%), Postives = 549/550 (99.82%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 550

BLAST of MS001254 vs. NCBI nr
Match: XP_022131773.1 (dentin sialophosphoprotein-like isoform X2 [Momordica charantia])

HSP 1 Score: 995.3 bits (2572), Expect = 2.0e-286
Identity = 531/550 (96.55%), Postives = 532/550 (96.73%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIA                 EQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIA-----------------EQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 533

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 533

BLAST of MS001254 vs. NCBI nr
Match: XP_022131774.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X3 [Momordica charantia])

HSP 1 Score: 987.6 bits (2552), Expect = 4.2e-284
Identity = 529/550 (96.18%), Postives = 530/550 (96.36%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGA                   GCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGA-------------------GCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 531

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 531

BLAST of MS001254 vs. NCBI nr
Match: XP_022131775.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X4 [Momordica charantia])

HSP 1 Score: 976.5 bits (2523), Expect = 9.7e-281
Identity = 524/550 (95.27%), Postives = 525/550 (95.45%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGA                        DPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGA------------------------DPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 526

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 526

BLAST of MS001254 vs. NCBI nr
Match: XP_022131776.1 (uncharacterized RING finger protein ECU07_0330-like isoform X5 [Momordica charantia])

HSP 1 Score: 955.3 bits (2468), Expect = 2.3e-274
Identity = 515/550 (93.64%), Postives = 516/550 (93.82%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELT             
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELT------------- 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
                               YEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 --------------------YEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 517

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 517

BLAST of MS001254 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 2.0e-31
Identity = 70/164 (42.68%), Postives = 102/164 (62.20%), Query Frame = 0

Query: 375 LARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFLSGLNFYDQHRDMRL 434
           L R+LA  +  R+  I+E+   L  +   E L +E  ++ +  ++      +D+HRDMRL
Sbjct: 508 LGRSLAADNDGRNRLISEIRQVLSAMRRGENLRFEDYMVFDPLIYQGMAEMHDRHRDMRL 567

Query: 435 DIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENTSAGSSEGSSEYEDG 494
           D+DNMSYEELLAL ER+G VST ++EE + + +       +H   +SA  S     ++D 
Sbjct: 568 DVDNMSYEELLALGERIGDVSTGLSEEVILKVM------KQHKHTSSAAGS-----HQDM 627

Query: 495 VKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICK 539
             CC+CQEEY+ GD++GTL C H +H  C++QWL LKN CPICK
Sbjct: 628 EPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICK 660

BLAST of MS001254 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 135.6 bits (340), Expect = 1.7e-30
Identity = 72/149 (48.32%), Postives = 99/149 (66.44%), Query Frame = 0

Query: 390 IAEVLLALERIEHEEELTYEQVILLETNLFLSGLNFYDQHRDMRLDIDNMSYEELLALEE 449
           + E+  ALE I   E + +E       ++F  G++ +D+HRDMRLDIDNMSYEELLALEE
Sbjct: 531 LIEIRNALEMIHRGENVRFE-------SIFYGGVDIHDRHRDMRLDIDNMSYEELLALEE 590

Query: 450 RMGSVSTAVTEEALSQCLNRSTYQSRHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDE 509
           R+G+VST ++EE +++ L +  +        S+   E S E E    CCICQEEY +GD+
Sbjct: 591 RIGNVSTGLSEEEVTKLLKQRKF--------SSWRLEASVEEE---PCCICQEEYVDGDD 650

Query: 510 VGTLQCEHTYHEGCIQQWLRLKNWCPICK 539
           +GTL C H +H GC++QWL +KN CPICK
Sbjct: 651 LGTLDCGHDFHVGCVRQWLVVKNTCPICK 661

BLAST of MS001254 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.5e-29
Identity = 68/165 (41.21%), Postives = 102/165 (61.82%), Query Frame = 0

Query: 375 LARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFLSGLNFYDQHRDMRL 434
           + R+LA   + R+  I+E+   L  +   E L  E  ++ +  ++    + +D+HR+MRL
Sbjct: 542 IGRSLAADGNGRNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRL 601

Query: 435 DIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENTSAGSSEGSSEYEDG 494
           D+DNMSYEELLAL ER+G VST ++EE + + + +  + S         SS  S E    
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTS---------SSPSSVELHQN 661

Query: 495 VK-CCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICK 539
           ++ CCICQEEY  GD +GTL+C H +H+ CI+QW+ +KN CPICK
Sbjct: 662 IEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICK 697

BLAST of MS001254 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.6e-26
Identity = 97/306 (31.70%), Postives = 148/306 (48.37%), Query Frame = 0

Query: 253 SSSERRITSSGYGVSISDSRGAKNGT----SPRENGIAGRSRASINGMSRARSHGNRPDR 312
           S S R +T+     + S SR     +     P  + +A  S  +     R R+  +   R
Sbjct: 394 SQSSRNVTNGNLNSASSVSRTGSTTSVPPPPPPSSNLAWTSYQNSPHYQRRRTERSELAR 453

Query: 313 NSTSLQESRSMISQVPHVH------QPDSSIDSNAHEVSTDLISDHPISYRRS----DSI 372
            S     +    +Q    H       P +S D    +   D    H  +Y R+    D  
Sbjct: 454 RSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGDNSQMHNRAYSRAGPLFDRQ 513

Query: 373 NENILG----NRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILL 432
            ++++G     R  + A  G +R + ++       +  VL  + R  +   L  E V+LL
Sbjct: 514 GDSVVGIPHPLRALAAASRGRSRLMVSQ-------MQNVLDVMRRDANNNNLRLEDVMLL 573

Query: 433 ETNLFLSGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQS 492
             ++   G   +D++RDMRLD+DNMSYEELLALEER+G V T V EE +S  L +  Y+S
Sbjct: 574 NHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNRLKQRKYKS 633

Query: 493 RHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWC 541
               NT +         +D   CC+CQEEY+ G+++GTL+C H +H  CI++WL+ KN C
Sbjct: 634 ----NTKSP--------QDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNLC 680

BLAST of MS001254 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.5e-21
Identity = 80/258 (31.01%), Postives = 124/258 (48.06%), Query Frame = 0

Query: 293 INGMSRARSHGNRPDRNST---SLQESRSMIS-----QVPHVHQPDSSIDSNAHEVSTDL 352
           +NGM+ A    +R     T   S +E ++  S      +P+ HQ       +AH +    
Sbjct: 266 VNGMNNAPGRFSRSGMTETVNGSFREYQNGPSTLCRGPLPYFHQ---HAGMHAHNLLDHT 325

Query: 353 ISDHPISYRRSDSINENILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHE- 412
               P     ++ +   +  N   +   +G    +   +S R +G     L    + H  
Sbjct: 326 QVQAPYQQCHNNPVLHGV--NHSGNRFHLGPRIPVLFSNSERTFGPPHHPLLANPVNHRN 385

Query: 413 -EELTYEQVILLETNLFLSGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEA 472
              L  E   +++ +      N  D+HRDMRLDID+M+YEELLALEE++G V+T + +  
Sbjct: 386 IRILPPEHATIMDFSRLYEVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSY 445

Query: 473 LSQCLNRSTYQSRHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEG 532
           + + L  S +       +S  S++ S    +   C ICQEEY   + +GTL C H YHE 
Sbjct: 446 IVEKLKTSLF----VPGSSCMSNKSSESSMENDACIICQEEYQVKECIGTLDCGHRYHED 505

Query: 533 CIQQWLRLKNWCPICKAS 541
           CI+QWL +KN CPICK +
Sbjct: 506 CIKQWLMVKNLCPICKTT 514

BLAST of MS001254 vs. ExPASy TrEMBL
Match: A0A6J1BRY9 (dentin sialophosphoprotein-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004854 PE=4 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 3.8e-299
Identity = 548/550 (99.64%), Postives = 549/550 (99.82%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 550

BLAST of MS001254 vs. ExPASy TrEMBL
Match: A0A6J1BUF6 (dentin sialophosphoprotein-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004854 PE=4 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 9.8e-287
Identity = 531/550 (96.55%), Postives = 532/550 (96.73%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIA                 EQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIA-----------------EQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 533

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 533

BLAST of MS001254 vs. ExPASy TrEMBL
Match: A0A6J1BR85 (probable E3 ubiquitin-protein ligase RHG1A isoform X3 OS=Momordica charantia OX=3673 GN=LOC111004854 PE=4 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 2.0e-284
Identity = 529/550 (96.18%), Postives = 530/550 (96.36%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGA                   GCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGA-------------------GCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 531

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 531

BLAST of MS001254 vs. ExPASy TrEMBL
Match: A0A6J1BQM5 (probable E3 ubiquitin-protein ligase RHG1A isoform X4 OS=Momordica charantia OX=3673 GN=LOC111004854 PE=4 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.7e-281
Identity = 524/550 (95.27%), Postives = 525/550 (95.45%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGA                        DPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGA------------------------DPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
           SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 526

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 526

BLAST of MS001254 vs. ExPASy TrEMBL
Match: A0A6J1BQG0 (uncharacterized RING finger protein ECU07_0330-like isoform X5 OS=Momordica charantia OX=3673 GN=LOC111004854 PE=4 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 515/550 (93.64%), Postives = 516/550 (93.82%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60
           MEEYPSKRAADGYVSRRGPG ILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL
Sbjct: 1   MEEYPSKRAADGYVSRRGPGAILRDTANNRDQDGKYCSRIGCSGRLNSPKGTRSSYSEKL 60

Query: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDEPE 120
           KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET+RKLSTQLETDSSETSSTQDEPE
Sbjct: 61  KSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETRRKLSTQLETDSSETSSTQDEPE 120

Query: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180
           ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG
Sbjct: 121 ISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKDTAAG 180

Query: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240
           SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA
Sbjct: 181 SSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKRNSEA 240

Query: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300
           ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR
Sbjct: 241 ESSSTGGKKMNGSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRASINGMSRAR 300

Query: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360
           SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE
Sbjct: 301 SHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPISYRRSDSINE 360

Query: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFL 420
           NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELT             
Sbjct: 361 NILGNRPASPAEIGLARTLATRDSFRHYGIAEVLLALERIEHEEELT------------- 420

Query: 421 SGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480
                               YEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT
Sbjct: 421 --------------------YEELLALEERMGSVSTAVTEEALSQCLNRSTYQSRHAENT 480

Query: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 540
           SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS
Sbjct: 481 SAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQWLRLKNWCPICKAS 517

Query: 541 VEEPASALPS 551
           VEEPASALPS
Sbjct: 541 VEEPASALPS 517

BLAST of MS001254 vs. TAIR 10
Match: AT5G10650.1 (RING/U-box superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 1.0e-78
Identity = 230/561 (41.00%), Postives = 317/561 (56.51%), Query Frame = 0

Query: 1   MEEYPSKRAADG-YVSRRGPGTILRDTANNRD-QDGKYCSRIGCSGRLNSPKGTR-SSYS 60
           M+    KR+ D   V R+  G  LR+  N  D ++  +CSR+GC+ ++ S K +R  S  
Sbjct: 1   MDGCAGKRSVDRLVVPRKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTD 60

Query: 61  EKLKSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET-QRKLSTQLETDSSETSSTQ 120
              K  L    +  + KE+VGSSSRT       +K    T +R+ S+ L+T+SSETS   
Sbjct: 61  NNTKVGLPPVPSTLNRKEIVGSSSRTPGGFGYLRKPAKVTARRQPSSSLDTESSETSCIH 120

Query: 121 DEPEISQ-VVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRG-RRDFNRRSGTRS 180
           D+P  ++  +P  +  R  ++V  +S+ S +  +    + A S++RG  R  + +S   +
Sbjct: 121 DDPAATEPTLPRQKTKRVTINVHPQSAVSREVVI----TKAGSSSRGTSRISHPKSELGT 180

Query: 181 KDTAAGSSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAK 240
           +D   G SV T   +S  T RGG  RH LRN  CNS+SDV+      ++ N + +    K
Sbjct: 181 RDALTGPSVSTSSGNSEHTVRGGLSRHRLRNLSCNSVSDVL-----PTNSNSATKISVTK 240

Query: 241 KRNSEAESS-STGGKKMN--GSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRA 300
           K+N++ ESS S+ G K +        +I+S G GV++SD+R  +N   P    I   S  
Sbjct: 241 KKNADGESSLSSKGSKTSVLVPKVRNQISSHGNGVTVSDNR--RNRVVP---SIRDSSTV 300

Query: 301 SINGMSRARSHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPIS 360
             NG  RA   G      +T+   S +   Q+PH   P +  D N             +S
Sbjct: 301 VSNGCRRAGYFGRSERLGATA---SSATSRQMPH---PTTPTDPNP-----------SLS 360

Query: 361 YRRSD--SINENILGNRPASPAEIGLARTLATRDSFRHY---GIAEVLLALERIEHEEEL 420
           +  S+  S    +  N P SP E   + +L  RD   HY   GIAEVLLALERIEH+EEL
Sbjct: 361 FCPSNIYSSTGRVHSNMPGSPTEADPSSSLVNRDGLSHYNMNGIAEVLLALERIEHDEEL 420

Query: 421 TYEQVILLETNLFLSGL-NFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQ 480
           TYEQ+  +ETNLF SG+  FYDQHRDMRLDIDNMSYEELLAL ++MG+VSTA++EEALS+
Sbjct: 421 TYEQLASIETNLFSSGMFRFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSR 480

Query: 481 CLNRSTYQSRHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQ 540
            L +S YQ    E    GS   S   +D +KC ICQEEY +GDE+GT+ C+H YH  C+Q
Sbjct: 481 SLKQSIYQ----ETDETGSI--SLYKDDDIKCSICQEEYVDGDELGTIPCQHMYHVSCVQ 524

Query: 541 QWLRLKNWCPICKASVEEPAS 547
           QWLR+KNWCPICK S EE  S
Sbjct: 541 QWLRMKNWCPICKTSAEEEKS 524

BLAST of MS001254 vs. TAIR 10
Match: AT5G10650.2 (RING/U-box superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 1.0e-78
Identity = 230/561 (41.00%), Postives = 317/561 (56.51%), Query Frame = 0

Query: 1   MEEYPSKRAADG-YVSRRGPGTILRDTANNRD-QDGKYCSRIGCSGRLNSPKGTR-SSYS 60
           M+    KR+ D   V R+  G  LR+  N  D ++  +CSR+GC+ ++ S K +R  S  
Sbjct: 1   MDGCAGKRSVDRLVVPRKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTD 60

Query: 61  EKLKSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAET-QRKLSTQLETDSSETSSTQ 120
              K  L    +  + KE+VGSSSRT       +K    T +R+ S+ L+T+SSETS   
Sbjct: 61  NNTKVGLPPVPSTLNRKEIVGSSSRTPGGFGYLRKPAKVTARRQPSSSLDTESSETSCIH 120

Query: 121 DEPEISQ-VVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRG-RRDFNRRSGTRS 180
           D+P  ++  +P  +  R  ++V  +S+ S +  +    + A S++RG  R  + +S   +
Sbjct: 121 DDPAATEPTLPRQKTKRVTINVHPQSAVSREVVI----TKAGSSSRGTSRISHPKSELGT 180

Query: 181 KDTAAGSSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAK 240
           +D   G SV T   +S  T RGG  RH LRN  CNS+SDV+      ++ N + +    K
Sbjct: 181 RDALTGPSVSTSSGNSEHTVRGGLSRHRLRNLSCNSVSDVL-----PTNSNSATKISVTK 240

Query: 241 KRNSEAESS-STGGKKMN--GSSSERRITSSGYGVSISDSRGAKNGTSPRENGIAGRSRA 300
           K+N++ ESS S+ G K +        +I+S G GV++SD+R  +N   P    I   S  
Sbjct: 241 KKNADGESSLSSKGSKTSVLVPKVRNQISSHGNGVTVSDNR--RNRVVP---SIRDSSTV 300

Query: 301 SINGMSRARSHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPIS 360
             NG  RA   G      +T+   S +   Q+PH   P +  D N             +S
Sbjct: 301 VSNGCRRAGYFGRSERLGATA---SSATSRQMPH---PTTPTDPNP-----------SLS 360

Query: 361 YRRSD--SINENILGNRPASPAEIGLARTLATRDSFRHY---GIAEVLLALERIEHEEEL 420
           +  S+  S    +  N P SP E   + +L  RD   HY   GIAEVLLALERIEH+EEL
Sbjct: 361 FCPSNIYSSTGRVHSNMPGSPTEADPSSSLVNRDGLSHYNMNGIAEVLLALERIEHDEEL 420

Query: 421 TYEQVILLETNLFLSGL-NFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQ 480
           TYEQ+  +ETNLF SG+  FYDQHRDMRLDIDNMSYEELLAL ++MG+VSTA++EEALS+
Sbjct: 421 TYEQLASIETNLFSSGMFRFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSR 480

Query: 481 CLNRSTYQSRHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQ 540
            L +S YQ    E    GS   S   +D +KC ICQEEY +GDE+GT+ C+H YH  C+Q
Sbjct: 481 SLKQSIYQ----ETDETGSI--SLYKDDDIKCSICQEEYVDGDELGTIPCQHMYHVSCVQ 524

Query: 541 QWLRLKNWCPICKASVEEPAS 547
           QWLR+KNWCPICK S EE  S
Sbjct: 541 QWLRMKNWCPICKTSAEEEKS 524

BLAST of MS001254 vs. TAIR 10
Match: AT5G24870.1 (RING/U-box superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-77
Identity = 223/555 (40.18%), Postives = 309/555 (55.68%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDG--KYCSRIGCSGRLNSPKGTRSSYSE 60
           M+ +  KR +   + R+  G +L +    +D       CSRIGCS R++S KG       
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKG--DLIDH 60

Query: 61  KLKSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKK-SVAETQRKLSTQLETDSSETSS-TQ 120
           K K+ + SFR+  SGKE VGSSSR+     G+KK S    +R+LS+ L+ DSSE+SS  +
Sbjct: 61  KAKATVSSFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNE 120

Query: 121 DEPEISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKD 180
           D P   + +P  +   + + V  +SS S +   E GSS+  +     R  ++R    S+D
Sbjct: 121 DSPTSERSLPRGKTKESTISVHSESSVSGEVVTEAGSSSRGTG----RSIHQRPDLVSRD 180

Query: 181 TAAGSSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKR 240
                       +S Q +R    ++ LR+ R  S SDV+    S+S P  +++ +  +K+
Sbjct: 181 ARV--------SNSEQNARASVNKNGLRDLRNKSGSDVLP---SNSTP--TRKSNIFRKK 240

Query: 241 NSEAESSSTG-GKKMNGSSSERRITSS--GYGVSISDSRGAKNGTSPRENGIAGRSRASI 300
            S+ ESSS+  G K  GS    +  SS  G G+++S+ R  +N  S R+N +   S    
Sbjct: 241 TSDGESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRNLPSVRDNSVVSSS---- 300

Query: 301 NGMSRARSHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPI-SY 360
                         R ST           V  +  P     ++ +   +   S  P+ SY
Sbjct: 301 -------------TRRSTGYYGRTGRAGAVATLQAPRPPTRADLNPSRSAEASRSPLNSY 360

Query: 361 RRSDSINENILG-NRPASPAEIGLARTLATRDSFRHY---GIAEVLLALERIEHEEELTY 420
            R  S N  +     P SP+E GL+R+L  RD+FR Y   G+AEVLLALERIE +EELTY
Sbjct: 361 SRPISSNGRLRSLMMPGSPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTY 420

Query: 421 EQVILLETNLFLSGL-NFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCL 480
           EQ+ +LETNLFL+G+ +F+DQHRDMRLDIDNMSYEELLALEE+MG+VSTA++EEAL + L
Sbjct: 421 EQLAVLETNLFLNGMSSFHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSL 480

Query: 481 NRSTYQSRHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQW 540
             S Y+          S +     +D VKC ICQEEY +GDEVGTL C+H YH  C QQW
Sbjct: 481 KSSIYRPNDE------SDDICLNKDDDVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQW 513

Query: 541 LRLKNWCPICKASVE 543
           LR+KNWCPICK S E
Sbjct: 541 LRMKNWCPICKTSAE 513

BLAST of MS001254 vs. TAIR 10
Match: AT5G24870.2 (RING/U-box superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-77
Identity = 223/555 (40.18%), Postives = 309/555 (55.68%), Query Frame = 0

Query: 1   MEEYPSKRAADGYVSRRGPGTILRDTANNRDQDG--KYCSRIGCSGRLNSPKGTRSSYSE 60
           M+ +  KR +   + R+  G +L +    +D       CSRIGCS R++S KG       
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKG--DLIDH 60

Query: 61  KLKSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKK-SVAETQRKLSTQLETDSSETSS-TQ 120
           K K+ + SFR+  SGKE VGSSSR+     G+KK S    +R+LS+ L+ DSSE+SS  +
Sbjct: 61  KAKATVSSFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNE 120

Query: 121 DEPEISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRSKD 180
           D P   + +P  +   + + V  +SS S +   E GSS+  +     R  ++R    S+D
Sbjct: 121 DSPTSERSLPRGKTKESTISVHSESSVSGEVVTEAGSSSRGTG----RSIHQRPDLVSRD 180

Query: 181 TAAGSSVVTGPKSSRQTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKRKDTAKKR 240
                       +S Q +R    ++ LR+ R  S SDV+    S+S P  +++ +  +K+
Sbjct: 181 ARV--------SNSEQNARASVNKNGLRDLRNKSGSDVLP---SNSTP--TRKSNIFRKK 240

Query: 241 NSEAESSSTG-GKKMNGSSSERRITSS--GYGVSISDSRGAKNGTSPRENGIAGRSRASI 300
            S+ ESSS+  G K  GS    +  SS  G G+++S+ R  +N  S R+N +   S    
Sbjct: 241 TSDGESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRNLPSVRDNSVVSSS---- 300

Query: 301 NGMSRARSHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHEVSTDLISDHPI-SY 360
                         R ST           V  +  P     ++ +   +   S  P+ SY
Sbjct: 301 -------------TRRSTGYYGRTGRAGAVATLQAPRPPTRADLNPSRSAEASRSPLNSY 360

Query: 361 RRSDSINENILG-NRPASPAEIGLARTLATRDSFRHY---GIAEVLLALERIEHEEELTY 420
            R  S N  +     P SP+E GL+R+L  RD+FR Y   G+AEVLLALERIE +EELTY
Sbjct: 361 SRPISSNGRLRSLMMPGSPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTY 420

Query: 421 EQVILLETNLFLSGL-NFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEALSQCL 480
           EQ+ +LETNLFL+G+ +F+DQHRDMRLDIDNMSYEELLALEE+MG+VSTA++EEAL + L
Sbjct: 421 EQLAVLETNLFLNGMSSFHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSL 480

Query: 481 NRSTYQSRHAENTSAGSSEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEGCIQQW 540
             S Y+          S +     +D VKC ICQEEY +GDEVGTL C+H YH  C QQW
Sbjct: 481 KSSIYRPNDE------SDDICLNKDDDVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQW 513

Query: 541 LRLKNWCPICKASVE 543
           LR+KNWCPICK S E
Sbjct: 541 LRMKNWCPICKTSAE 513

BLAST of MS001254 vs. TAIR 10
Match: AT4G31450.1 (RING/U-box superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 8.2e-60
Identity = 206/560 (36.79%), Postives = 289/560 (51.61%), Query Frame = 0

Query: 1   MEEYPSKRAADGYV-SRRGPGTILRDTANNRDQDGKYCSRIGCSGRLNSPKGTR--SSYS 60
           M+ + SKRA +    S++  G + RD  N+       C    CS    S   TR   S  
Sbjct: 1   MDRWSSKRAMEARPDSKKKGGVVFRDRFNSNS-----CKVPICSDEKKSMNFTRFVGSSD 60

Query: 61  EKLKSPLQSFRTNSSGKEVVGSSSRTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQD 120
           +K KS L ++R++ +GKEV+G+SS+   +   S  SV   +++  +Q+  DSSE+S   +
Sbjct: 61  KKEKSVLSTYRSSPNGKEVIGTSSKICIS---SSSSVKSGEKQPFSQIAIDSSESSRGSE 120

Query: 121 ---EPEISQVVPSNERIRTGLHVGFKSSDSADDTMEPGSSNAASNTRGRRDFNRRSGTRS 180
              E EI QV    ++ R    +  K       T E   S   S++R +R F +R G   
Sbjct: 121 DEVESEILQVPLGRDKRR----MNNKLIYGKVITPEAECSKLPSSSRIKRGFRQRFGL-- 180

Query: 181 KDTAAGSSVVTGPKSSRQTSRGGAGRHTLRNFRCNS-ISDVISSGCSSSDPNLSKRKDTA 240
                         S ++   G +G+ T  N  C+  +S VI SG    D  LS++ DT 
Sbjct: 181 --------------SKQEFHPGPSGQSTSANRGCSPLLSGVIPSG-FGLDKRLSRKADTI 240

Query: 241 KKRNSEAESSSTGGKKMNGSSS----ERRITSSGYGVSISDSRGAKNGTSPRENGIA--G 300
            K     ESSS+   +    +     E R  S     S+SDSR A++     +N +A  G
Sbjct: 241 SKTKVYGESSSSSSARGKNVTEPPPVEVRRRSFNPRGSVSDSRRARHCILDDDNDVASVG 300

Query: 301 RSRASINGMSRARSHGNRPDRNSTSLQESRSMISQVPHVHQPDSSIDSNAHE-VSTDLIS 360
             R +    SR R  G             R  +S V       +   +N +  VS +L S
Sbjct: 301 SQRLANRNNSRIRGSG-------------RDGLSSVTAAEMSQTETSNNLNSPVSLELFS 360

Query: 361 DHPISYRRSDSINENILGNRPASPAEIGLARTLATRDSFRHY---GIAEVLLALERIEHE 420
             P                      E GL+ +L + DSFR Y   GI+E+L  L+RIE +
Sbjct: 361 GFP----------------------EFGLSGSLLSHDSFRSYNLDGISEILPELDRIEQD 420

Query: 421 EELTYEQVILLETNLFLSGLNFYDQHRDMRLDIDNMSYEELLALEERMGSVSTAVTEEAL 480
            EL YE ++++ET L L GL+F+DQHRDMRLDIDNMSYEELLALEER+G+VSTA+TEEA+
Sbjct: 421 IELNYEDLLIMETGLLLGGLSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAI 480

Query: 481 SQCLNRSTYQSRHAENTSAGS-SEGSSEYEDGVKCCICQEEYSNGDEVGTLQCEHTYHEG 540
           S+CL  S YQ +     S GS ++  S+ ++  KC ICQEEY+ GDEVG L CEHTYH  
Sbjct: 481 SKCLKTSIYQMK---PLSYGSITKSPSDNKEDAKCSICQEEYTIGDEVGRLHCEHTYHVK 493

Query: 541 CIQQWLRLKNWCPICKASVE 543
           C+Q+WLR+K+WCPICKA+ E
Sbjct: 541 CVQEWLRIKSWCPICKATAE 493

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131770.17.9e-29999.64dentin sialophosphoprotein-like isoform X1 [Momordica charantia] >XP_022131771.1... [more]
XP_022131773.12.0e-28696.55dentin sialophosphoprotein-like isoform X2 [Momordica charantia][more]
XP_022131774.14.2e-28496.18probable E3 ubiquitin-protein ligase RHG1A isoform X3 [Momordica charantia][more]
XP_022131775.19.7e-28195.27probable E3 ubiquitin-protein ligase RHG1A isoform X4 [Momordica charantia][more]
XP_022131776.12.3e-27493.64uncharacterized RING finger protein ECU07_0330-like isoform X5 [Momordica charan... [more]
Match NameE-valueIdentityDescription
O495002.0e-3142.68E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q7XTV71.7e-3048.32Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9ZQF92.5e-2941.21E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q9FMM42.6e-2631.70Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q5QLR52.5e-2131.01Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1BRY93.8e-29999.64dentin sialophosphoprotein-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1BUF69.8e-28796.55dentin sialophosphoprotein-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1BR852.0e-28496.18probable E3 ubiquitin-protein ligase RHG1A isoform X3 OS=Momordica charantia OX=... [more]
A0A6J1BQM54.7e-28195.27probable E3 ubiquitin-protein ligase RHG1A isoform X4 OS=Momordica charantia OX=... [more]
A0A6J1BQG01.1e-27493.64uncharacterized RING finger protein ECU07_0330-like isoform X5 OS=Momordica char... [more]
Match NameE-valueIdentityDescription
AT5G10650.11.0e-7841.00RING/U-box superfamily protein [more]
AT5G10650.21.0e-7841.00RING/U-box superfamily protein [more]
AT5G24870.11.5e-7740.18RING/U-box superfamily protein [more]
AT5G24870.21.5e-7740.18RING/U-box superfamily protein [more]
AT4G31450.18.2e-6036.79RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 497..537
e-value: 9.4E-7
score: 38.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 496..538
e-value: 3.2E-11
score: 43.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 497..538
score: 11.884405
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 481..548
e-value: 3.9E-18
score: 66.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 306..321
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..321
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..87
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..242
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 243..285
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..226
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..161
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 1..542
NoneNo IPR availablePANTHERPTHR22937:SF136E3 UBIQUITIN-PROTEIN LIGASE ATL44-RELATEDcoord: 1..542
NoneNo IPR availableCDDcd16481RING-H2_TTC3coord: 496..538
e-value: 1.95408E-16
score: 71.2241
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 496..544

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001254.1MS001254.1mRNA