MS001196 (gene) Bitter gourd (TR) v1

Overview


Name	MS001196
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Malic enzyme
Location	scaffold36: 2204668 .. 2208515 (-)
RNA-Seq Expression	MS001196
Synteny	MS001196

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGGGCAATGAAGAAGACCATGAGCAATGGCAGTGTTCATATGGAGAGGGAGGAGATTGATAACAAGTTTGAGGAAGCCTATGGGGAACATAATGCCACGGAAGATCAGCTCATTACCCCTTGGACTGTCTCTGTTGCTAGGTAAGATTAAGAAAAGAAAAAAAAAACATGTTTTGCCTTTTTTTGGTCTGTCTTTGCGTTTGTTCTTACTGAAACTACTCTGCATTTGGGCTATCTGAATGAGTGATTCTCTGTTTTGTTGTTGTAGTGGTTACTCGTTACTGCGTGATCCGATCTACAACAAAGGCCTTGCATTTTCTGAGAAGGAGAGAGATGCTCATTACTTGCGTGGCTTGCTGCCTCCAGCAATCCTTGATCAGGAGCTTCAGGTTAGCCCAGATCTCGACAACTTATTTGAGGGGCTGGAACAATGTTGCTGATGATAAGTTCGATGTGTACAAGAGAGAAATTAAAAACTGTATTTCGAACTTGCATCCAACGGTTACTAAGGACTAATAGAATAGATTTTGAATCTGCATCGAATGGTTATTAGGAATCAACCACGTCTGGTCGACTAATGGCTCTCTCCCCCCCCTGTTTTTTCTTCTTTTGGCTTGCAGGAAAGGAGGATGATGCACAATCTTCGCCAATATGATGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGTTTATATTTTCTTTACTCATTTGTTATTAGTTCCTAACCATCATTAGCCTGCACAAAAACAAAGACAGTAAATCTTAGACACAAATTGATGTTTGTAATTTGGAGCTTAGTTCAACTTGGGCATGCCAAAGCTTTCCCATTGTTTCTGATATCATCTGTGATCTTGCAGGAGAGAAATGAGAGGCTGTTCTACAAGCTTCTCATTGACAATGTTGAAGAACTGCTTCCAGTTGTGTACACTCCCACAGTTGGTGAAGCCTGCCAGAAGTATGGAAGCATTTACAGGCGACCTCAGGGCCTTTTCATCAGTTTGAAAGAGAAGTATGCCATTAGAAAAGCACTTATTATGTTTGTTAGTTGCATTGGTTCAGAACTGATAATCATTTTCCTCTTCAGGGGAAAGATTCTTGAAGTACTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATCGTTGTGACCGACGGCGAGCGGATTCTCGGTCTTGGGGATCTCGGATGCCAGGTATAAAACTTACTGCTTCCATTCACCTGATGAACCTTAATTCACTTAGCTTCTATCTTTTAGTTTACTGTTTACATATTCTCAATTTGTTAAGATGGCATCTTTCAGGGCATGGGGATTCCTGTGGGAAAGCTTTCTTTGTATACCGCTCTTGGAGGAATTCGGCCCTCAGCTGTATGAATCTCTGCTCTCTTTGTTTATTATCAGTCATTACAGAACACTAATCTCAATTTAGCAAAATGTTTATACCTGGATTACTCAGCTTTGTTTTCAAGTACTAAATGTAAGTAAGGAATGTTGTTGAACTTTGTAGTTATATATTGTATGCAGTGCTTGCCTATTACTATCGATGTCGGTACGAACAACGAGCAGCTTTTGAATGACGAGTTCTACATTGGCCTTCGACATAAACGAGCCAGAGGACAGGTGAAACAATCTTTCATTCTTTTACTTGTGCTTTTATTCTCGTGCCTCCTCGATCTCAATCTGCTCATATATGATCGTTTTACTTAGTTTTGTGTGTGTTTTCCTTCATTCAGGAATACATGGAGCTTTTAGATGAGTTCATGTATGCAGTAAAGAAGAACTATGGAGAGAAAGTTCTAATACAGGTCATTTGGGCAATTTCAACTTACTCTAGTACTCTGCCTGGATTTTTTCAATTTTTATATGTGATTCAATGTGTCACTAAGCATTTCTTACTTATTTAACAGTTTGAAGATTTTGCAAACCACAATGCGTTCGAGTTGCTTTCGAGATATAGTTCGTCGCACCTTGTGTTTAATGACGACATTCAGGTATGAAATATACACACCTCTTTCAGTTAACTGTGAATTTTTATAAGTTTCCCGAAGTTGTTCTATAACATGGATCTTCTCATTATATCATTTAGGGTACAGCATCTGTCGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGAACCTTAACTGACCACACTTTCCTGTTCCTGGGAGCTGGAGAGGTGAGTCAAAAATCATAGTTATAATCAAAGTTTTCTTTAACCGTGTTCGTATTCTTCGGCACACATAACCTCGATGTATCATTTGTTTATTTGTGTCGTTAGAGATTGAATAGCCATGATTTTACTTGAACTTCCCTCCATTGTGCTGTGGAAATGGAATGGTGTATTGTTTCTTATTGGTGTCATTTGTCTGTTGTAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGGTACAAATATACTTCTATCTCTAATCTTCTTTGAATCAAATGAATTCATGGCTTATGATTTTGTTTTCTTTGGCAGACAAAAGCTCCAGTTGAAGAGACTCGCAGGAAGATTTGGCTTGTGGACTCAAAGGTTGCTTGGACTGACTTCATTTCAATATTGTGCCTTTGCACCTCAATGTTATTTCCTGTACTAAAAGAATTATAACCATTATTACAGGGGTTGATCGTTCGATCCCGCTTCGAATCCCTTCAACACTTCAAGAAGCCTTGGGCTCATGACCATGAATCCATCAAGGATCTCTTGGGTGCCGTCCAGGTCTGTAATCGACTTACTTTACTACTCACCCCTCGAAGTATATTGGATCGTTTTCTAAACCAATCTTAATCATACAGGCCATCAAGCCAACAGTGTTGATAGGAACATCTGGTGTTGGAAAGACATTCACAAAGGAGGTTGTGGAGGCCATGGCATCCCTCAATGAGGTTCACCCCTATTCCCTCCAACTTCTTCCCCCTCCCCCGACAAAAGAAATTATCTAAGATTAACCCGAGTTTCGGTTACTATGCAGAAACCACTTATCCTTGCTCTCTCGAACCCGACATCACAATCGGAGTGTACAGCCGAAGAAGCTTACACATGGAGCAAGGTACAAAATATGCTCCTATCTCTTCAAGTCTTTCAAAACCTCAAGCATCACTTAGGTTTGGAGCTTAGTTCTAAAACTTATCCTTTCAAATGTGCAGGGCCGGGCGATCTATGCCAGTGGAAGTCCATTTGACCCTGTTGAATATGATGGGAAAGTTTTTGTGCCTGGGCAGGTACCCTTCATACTTCAAAAAACCTTGCTTTAACAAGATGGTATTGGTATATGATCTTCTGTGCTAACTTCTCCTAATGTAATTCAGGCCAACAATGCTTACATATTCCCTGGATTCGGCTTGGGTCTGATCATGTCTGGTACGATTCGTGTGCACGATGACATGCTCTTGGCAGCCTGTAAGTGAAAATTTTAACTTCCCTCCTACGTATCTTGAGTATTAGTTGCTGGTATGAAGGTCGACTTAACGGTTTTTCCCGTTTGCAGCGGAGGCTCTGGCTGCCGAAGTGAGTCAGGAAAACTATGACAAGGGACTGATTTACCCTCCTTTTACCAATATCAGAAAGATATCTGCTAACATTGCTGCTAAAGTTGCAGCTAAAGCTTATGAGCTTGGTAAGCTTCTCTCCCATTTTCTTCACAATGTGATTAATCATGATTTCCTGATAAACCAATGCCAGAAAACTTTACTAACAGTTGCATCATATACAATTATATTACAGGTTTGGCTTCTCGTCTTCCTCGACCCAAGGATCTCGTAAAATTTGCGGAGAGCTGCATGTACAGCCCGCGCTACCGTAACTACCTT

mRNA sequence

ATGGAGGGGGCAATGAAGAAGACCATGAGCAATGGCAGTGTTCATATGGAGAGGGAGGAGATTGATAACAAGTTTGAGGAAGCCTATGGGGAACATAATGCCACGGAAGATCAGCTCATTACCCCTTGGACTGTCTCTGTTGCTAGTGGTTACTCGTTACTGCGTGATCCGATCTACAACAAAGGCCTTGCATTTTCTGAGAAGGAGAGAGATGCTCATTACTTGCGTGGCTTGCTGCCTCCAGCAATCCTTGATCAGGAGCTTCAGGAAAGGAGGATGATGCACAATCTTCGCCAATATGATGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAGAGGCTGTTCTACAAGCTTCTCATTGACAATGTTGAAGAACTGCTTCCAGTTGTGTACACTCCCACAGTTGGTGAAGCCTGCCAGAAGTATGGAAGCATTTACAGGCGACCTCAGGGCCTTTTCATCAGTTTGAAAGAGAAGTATGCCATTAGAAAAGCACTTATTATGTTTGTTAGTTGCATTGGTTCAGAACTGATAATCATTTTCCTCTTCAGGGGAAAGATTCTTGAAGTACTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATCGTTGTGACCGACGGCGAGCGGATTCTCGGTCTTGGGGATCTCGGATGCCAGGGCATGGGGATTCCTGTGGGAAAGCTTTCTTTGTATACCGCTCTTGGAGGAATTCGGCCCTCAGCTTGCTTGCCTATTACTATCGATGTCGGTACGAACAACGAGCAGCTTTTGAATGACGAGTTCTACATTGGCCTTCGACATAAACGAGCCAGAGGACAGGAATACATGGAGCTTTTAGATGAGTTCATGTATGCAGTAAAGAAGAACTATGGAGAGAAAGTTCTAATACAGTTTGAAGATTTTGCAAACCACAATGCGTTCGAGTTGCTTTCGAGATATAGTTCGTCGCACCTTGTGTTTAATGACGACATTCAGGGTACAGCATCTGTCGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGAACCTTAACTGACCACACTTTCCTGTTCCTGGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGACAAAAGCTCCAGTTGAAGAGACTCGCAGGAAGATTTGGCTTGTGGACTCAAAGGGGTTGATCGTTCGATCCCGCTTCGAATCCCTTCAACACTTCAAGAAGCCTTGGGCTCATGACCATGAATCCATCAAGGATCTCTTGGGTGCCGTCCAGGCCATCAAGCCAACAGTGTTGATAGGAACATCTGGTGTTGGAAAGACATTCACAAAGGAGGTTGTGGAGGCCATGGCATCCCTCAATGAGAAACCACTTATCCTTGCTCTCTCGAACCCGACATCACAATCGGAGTGTACAGCCGAAGAAGCTTACACATGGAGCAAGGGCCGGGCGATCTATGCCAGTGGAAGTCCATTTGACCCTGTTGAATATGATGGGAAAGTTTTTGTGCCTGGGCAGGCCAACAATGCTTACATATTCCCTGGATTCGGCTTGGGTCTGATCATGTCTGGTACGATTCGTGTGCACGATGACATGCTCTTGGCAGCCTCGGAGGCTCTGGCTGCCGAAGTGAGTCAGGAAAACTATGACAAGGGACTGATTTACCCTCCTTTTACCAATATCAGAAAGATATCTGCTAACATTGCTGCTAAAGTTGCAGCTAAAGCTTATGAGCTTGGTTTGGCTTCTCGTCTTCCTCGACCCAAGGATCTCGTAAAATTTGCGGAGAGCTGCATGTACAGCCCGCGCTACCGTAACTACCTT

Coding sequence (CDS)

ATGGAGGGGGCAATGAAGAAGACCATGAGCAATGGCAGTGTTCATATGGAGAGGGAGGAGATTGATAACAAGTTTGAGGAAGCCTATGGGGAACATAATGCCACGGAAGATCAGCTCATTACCCCTTGGACTGTCTCTGTTGCTAGTGGTTACTCGTTACTGCGTGATCCGATCTACAACAAAGGCCTTGCATTTTCTGAGAAGGAGAGAGATGCTCATTACTTGCGTGGCTTGCTGCCTCCAGCAATCCTTGATCAGGAGCTTCAGGAAAGGAGGATGATGCACAATCTTCGCCAATATGATGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAGAGGCTGTTCTACAAGCTTCTCATTGACAATGTTGAAGAACTGCTTCCAGTTGTGTACACTCCCACAGTTGGTGAAGCCTGCCAGAAGTATGGAAGCATTTACAGGCGACCTCAGGGCCTTTTCATCAGTTTGAAAGAGAAGTATGCCATTAGAAAAGCACTTATTATGTTTGTTAGTTGCATTGGTTCAGAACTGATAATCATTTTCCTCTTCAGGGGAAAGATTCTTGAAGTACTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATCGTTGTGACCGACGGCGAGCGGATTCTCGGTCTTGGGGATCTCGGATGCCAGGGCATGGGGATTCCTGTGGGAAAGCTTTCTTTGTATACCGCTCTTGGAGGAATTCGGCCCTCAGCTTGCTTGCCTATTACTATCGATGTCGGTACGAACAACGAGCAGCTTTTGAATGACGAGTTCTACATTGGCCTTCGACATAAACGAGCCAGAGGACAGGAATACATGGAGCTTTTAGATGAGTTCATGTATGCAGTAAAGAAGAACTATGGAGAGAAAGTTCTAATACAGTTTGAAGATTTTGCAAACCACAATGCGTTCGAGTTGCTTTCGAGATATAGTTCGTCGCACCTTGTGTTTAATGACGACATTCAGGGTACAGCATCTGTCGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGAACCTTAACTGACCACACTTTCCTGTTCCTGGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGACAAAAGCTCCAGTTGAAGAGACTCGCAGGAAGATTTGGCTTGTGGACTCAAAGGGGTTGATCGTTCGATCCCGCTTCGAATCCCTTCAACACTTCAAGAAGCCTTGGGCTCATGACCATGAATCCATCAAGGATCTCTTGGGTGCCGTCCAGGCCATCAAGCCAACAGTGTTGATAGGAACATCTGGTGTTGGAAAGACATTCACAAAGGAGGTTGTGGAGGCCATGGCATCCCTCAATGAGAAACCACTTATCCTTGCTCTCTCGAACCCGACATCACAATCGGAGTGTACAGCCGAAGAAGCTTACACATGGAGCAAGGGCCGGGCGATCTATGCCAGTGGAAGTCCATTTGACCCTGTTGAATATGATGGGAAAGTTTTTGTGCCTGGGCAGGCCAACAATGCTTACATATTCCCTGGATTCGGCTTGGGTCTGATCATGTCTGGTACGATTCGTGTGCACGATGACATGCTCTTGGCAGCCTCGGAGGCTCTGGCTGCCGAAGTGAGTCAGGAAAACTATGACAAGGGACTGATTTACCCTCCTTTTACCAATATCAGAAAGATATCTGCTAACATTGCTGCTAAAGTTGCAGCTAAAGCTTATGAGCTTGGTTTGGCTTCTCGTCTTCCTCGACCCAAGGATCTCGTAAAATTTGCGGAGAGCTGCATGTACAGCCCGCGCTACCGTAACTACCTT

Protein sequence

MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGSELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRNYL

Homology

[Close]

BLAST of MS001196 vs. NCBI nr
Match: XP_022132027.1 (NADP-dependent malic enzyme [Momordica charantia])

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 588/614 (95.77%), Postives = 588/614 (95.77%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 21  MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 80

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL
Sbjct: 81  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 140

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK               
Sbjct: 141 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK--------------- 200

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 201 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 260

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 261 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 320

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL
Sbjct: 321 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 380

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH
Sbjct: 381 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 440

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAM SLNEKPLILALSNPTSQSECTAE
Sbjct: 441 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMESLNEKPLILALSNPTSQSECTAE 500

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 501 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 560

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 561 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 609

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSPRYRNYL
Sbjct: 621 AESCMYSPRYRNYL 609

[Close]

BLAST of MS001196 vs. NCBI nr
Match: XP_022951634.1 (NADP-dependent malic enzyme [Cucurbita moschata] >XP_023002278.1 NADP-dependent malic enzyme [Cucurbita maxima])

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 568/614 (92.51%), Postives = 585/614 (95.28%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MEGAMKKTMSNGSVHME EEIDNK+EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 18  MEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 77

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAF+EKERDAHYLRGLLPPA+L+QELQERRMMHNLR Y+VPLHRYIAMMDLQERNERL
Sbjct: 78  KGLAFTEKERDAHYLRGLLPPALLNQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 137

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK               
Sbjct: 138 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK--------------- 197

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 198 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 257

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 258 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 317

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLL+ALKL+GGTL DHTFLFL
Sbjct: 318 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFL 377

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALE+SKQT+APVEETR+KIWLVDSKGLIVRSRFESLQHFKKPWAHDH
Sbjct: 378 GAGEAGTGIAELIALEVSKQTQAPVEETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 437

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE
Sbjct: 438 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 497

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWS+GRAI+ASGSPFDPVEYDGKV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 498 EAYTWSQGRAIFASGSPFDPVEYDGKVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 557

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAA+VS+ENY+KGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 558 AASEALAAQVSEENYEKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 606

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSP YR+YL
Sbjct: 618 AESCMYSPGYRSYL 606

[Close]

BLAST of MS001196 vs. NCBI nr
Match: KAG7020293.1 (NADP-dependent malic enzyme [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 567/614 (92.35%), Postives = 584/614 (95.11%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MEGAMKKTMSNGSVHME EEIDNK+EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 18  MEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 77

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAF+EKERDAHYLRGLLPPA+L+QELQERRMMHNLR Y+VPLHRYIAMMDLQERNERL
Sbjct: 78  KGLAFTEKERDAHYLRGLLPPALLNQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 137

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK               
Sbjct: 138 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK--------------- 197

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 198 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 257

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 258 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 317

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLL+ALKL+GGTL DHTFLFL
Sbjct: 318 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFL 377

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALE+SKQT+APVEETR+KIWLVDSKGLIVRSRFESLQHFKKPWAHDH
Sbjct: 378 GAGEAGTGIAELIALEVSKQTQAPVEETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 437

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMAS NEKPLILALSNPTSQSECTAE
Sbjct: 438 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAE 497

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWS+GRAI+ASGSPFDPVEYDGKV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 498 EAYTWSQGRAIFASGSPFDPVEYDGKVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 557

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAA+VS+ENY+KGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 558 AASEALAAQVSEENYEKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 606

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSP YR+YL
Sbjct: 618 AESCMYSPGYRSYL 606

[Close]

BLAST of MS001196 vs. NCBI nr
Match: KAG6585377.1 (NADP-dependent malic enzyme, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 566/614 (92.18%), Postives = 584/614 (95.11%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MEGAMKKTMSNGSVHME EEIDNK+EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 18  MEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 77

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAF+EKERDAHYLRGLLPPA+L+QELQERRMMHNLR Y+VPLHRYIAMMDLQERNERL
Sbjct: 78  KGLAFTEKERDAHYLRGLLPPALLNQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 137

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK               
Sbjct: 138 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK--------------- 197

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 198 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 257

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 258 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 317

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLL+ALKL+GGTL DHTFLFL
Sbjct: 318 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFL 377

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALE+SKQT++PVEETR+KIWLVDSKGLIVRSRFESLQHFKKPWAHDH
Sbjct: 378 GAGEAGTGIAELIALEVSKQTQSPVEETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 437

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMAS NEKPLILALSNPTSQSECTAE
Sbjct: 438 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAE 497

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWS+GRAI+ASGSPFDPVEYDGKV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 498 EAYTWSQGRAIFASGSPFDPVEYDGKVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 557

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAA+VS+ENY+KGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 558 AASEALAAQVSEENYEKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 606

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSP YR+YL
Sbjct: 618 AESCMYSPGYRSYL 606

[Close]

BLAST of MS001196 vs. NCBI nr
Match: XP_011649727.1 (NADP-dependent malic enzyme isoform X1 [Cucumis sativus] >KGN63047.2 hypothetical protein Csa_022109 [Cucumis sativus])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 567/614 (92.35%), Postives = 580/614 (94.46%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           ME +MKKTMSNGSVHM+ EEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 28  MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPA++DQELQERRMMHNLR Y+VPLHRYIAMMDLQERNERL
Sbjct: 88  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 147

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEK               
Sbjct: 148 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEK--------------- 207

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 208 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 267

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLR KRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 268 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKV 327

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTL DHTFLFL
Sbjct: 328 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFL 387

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALEISKQT AP EETR+KIWLVDSKGLIV+SRFESLQHFKKPWAHDH
Sbjct: 388 GAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDH 447

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDL GAVQAIKPTVLIGTSGVGKTFTKEVVEAMAS NEKPLILALSNPTSQSECTAE
Sbjct: 448 ESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAE 507

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWS+GRAI+ASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 508 EAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 567

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAA+VSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 568 AASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 616

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSPRYR+YL
Sbjct: 628 AESCMYSPRYRSYL 616

[Close]

BLAST of MS001196 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 6.8e-286
Identity = 495/612 (80.88%), Postives = 540/612 (88.24%), Query Frame = 0


Query: 2   EGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNK 61
           E ++KK  SNG              + YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 12  EVSVKKDYSNG----------GGVRDLYGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 71

Query: 62  GLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLF 121
           GLAF+E ERDAHYLRGLLPP++ +QELQE+R+MHNLRQY+VPLHRY+A+MDLQERNERLF
Sbjct: 72  GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 131

Query: 122 YKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGS 181
           YKLLIDNV ELLPVVYTPTVGEACQKYGSI+RRPQGL+ISLKEK                
Sbjct: 132 YKLLIDNVAELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEK---------------- 191

Query: 182 ELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALG 241
                    GKILEVLKNWPE++IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALG
Sbjct: 192 ---------GKILEVLKNWPEKSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALG 251

Query: 242 GIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVL 301
           G+RPS+CLP+TIDVGTNNE+LLNDEFYIGLR +RA GQEY   LDEFM AVK+NYGEKVL
Sbjct: 252 GVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQRRATGQEYATFLDEFMRAVKQNYGEKVL 311

Query: 302 IQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLG 361
           +QFEDFANHNAF+LL +YSSSHLVFNDDIQGTASVV+AGLLA+LKL+GGTL DHTFLFLG
Sbjct: 312 VQFEDFANHNAFDLLEKYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLG 371

Query: 362 AGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHE 421
           AGEAGTGIAELIA+E+SKQTKAPVEETR+KIWLVDSKGLIV SR ESLQ FKKPWAH+HE
Sbjct: 372 AGEAGTGIAELIAVEVSKQTKAPVEETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHE 431

Query: 422 SIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEE 481
            +K LL AV+AIKPTVLIG+SG GKTFTKEVVE MASLNEKPLILALSNPTSQSECTAEE
Sbjct: 432 PVKGLLEAVKAIKPTVLIGSSGAGKTFTKEVVETMASLNEKPLILALSNPTSQSECTAEE 491

Query: 482 AYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLA 541
           AYTWSKGRAI+ASGSPFDPVEY+GK+FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLA
Sbjct: 492 AYTWSKGRAIFASGSPFDPVEYEGKLFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLA 551

Query: 542 ASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFA 601
           ASEALAA+VS+ENYDKGLIYPPFTNIRKISANIAAKVAAKAY+LGLAS L RPKDLVK+A
Sbjct: 552 ASEALAAQVSEENYDKGLIYPPFTNIRKISANIAAKVAAKAYDLGLASHLKRPKDLVKYA 588

Query: 602 ESCMYSPRYRNY 614
           ESCMYSP YR+Y
Sbjct: 612 ESCMYSPGYRSY 588

[Close]

BLAST of MS001196 vs. ExPASy Swiss-Prot
Match: Q9XGZ0 (NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME3 PE=1 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 1.0e-273
Identity = 470/611 (76.92%), Postives = 524/611 (85.76%), Query Frame = 0


Query: 3   GAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKG 62
           G  +  +S+  V      +     + YGE +AT DQL+TPW  SVASGY+L+RDP YNKG
Sbjct: 2   GTNQTQISDEYVTGNSSGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKG 61

Query: 63  LAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLFY 122
           LAF++KERDAHY+ GLLPP +L Q++QER++MHNLRQY VPL RY+A+MDLQERNERLFY
Sbjct: 62  LAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFY 121

Query: 123 KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGSE 182
           KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGL+ISLKEK                 
Sbjct: 122 KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEK----------------- 181

Query: 183 LIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 242
                   GKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG
Sbjct: 182 --------GKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 241

Query: 243 IRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLI 302
           IRPSACLPITIDVGTNNE+LLN+EFYIGL+ KRA G+EY E L EFM AVK+NYGEKVL+
Sbjct: 242 IRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCAVKQNYGEKVLV 301

Query: 303 QFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGA 362
           QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +L DHTFLFLGA
Sbjct: 302 QFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLADHTFLFLGA 361

Query: 363 GEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHES 422
           GEAGTGIAELIAL+ISK+T  P++ETR+KIWLVDSKGLIV  R ESLQHFK+PWAHDH+ 
Sbjct: 362 GEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWAHDHKP 421

Query: 423 IKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEA 482
           +K+LL AV AIKPTVLIGTSGVGKTFTKEVVEAMA+LNEKPLILALSNPTSQ+ECTAEEA
Sbjct: 422 VKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNPTSQAECTAEEA 481

Query: 483 YTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAA 542
           YTW+KGRAI+ASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG IRV DDMLLAA
Sbjct: 482 YTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAA 541

Query: 543 SEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAE 602
           SEALA++V++EN+  GLIYPPF NIRKISANIAA V AK YELGLAS LPRPKDLVK AE
Sbjct: 542 SEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNLPRPKDLVKMAE 587

Query: 603 SCMYSPRYRNY 614
           SCMYSP YRN+
Sbjct: 602 SCMYSPVYRNF 587

[Close]

BLAST of MS001196 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 3.0e-273
Identity = 464/620 (74.84%), Postives = 539/620 (86.94%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREE-------IDNKFEEAYGEHNATEDQLITPWTVSVASGYSL 60
           ++GA+K   +  +V +   E       +    E+ YGE +ATED  ITPW+VSVASGYSL
Sbjct: 52  VDGAVKDVNAPVAVEVADSESKKPTAVVGGGVEDVYGEDSATEDHFITPWSVSVASGYSL 111

Query: 61  LRDPIYNKGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDL 120
           LRDP +NKGLAF+EKERDAHYLRGLLPP +++ +LQ ++MMHN+RQY+VPL RY AMMDL
Sbjct: 112 LRDPHHNKGLAFTEKERDAHYLRGLLPPVVVNHDLQVKKMMHNIRQYEVPLQRYQAMMDL 171

Query: 121 QERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALI 180
           QERNERLFYKLLI+N+EELLP+VYTPTVGEACQKYG+I++ PQGL+ISLK+K        
Sbjct: 172 QERNERLFYKLLIENIEELLPIVYTPTVGEACQKYGTIFKNPQGLYISLKDK-------- 231

Query: 181 MFVSCIGSELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGK 240
                            GK+LE+LKNWP++ IQVIVVTDGERILGLGDLGCQGMGIPVGK
Sbjct: 232 -----------------GKVLEILKNWPQKKIQVIVVTDGERILGLGDLGCQGMGIPVGK 291

Query: 241 LSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVK 300
           LSLYTALGGIRPSACLPITIDVGTNNE++LNDEFYIGLR +RA G+EY EL++EFM AVK
Sbjct: 292 LSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEYAELMNEFMSAVK 351

Query: 301 KNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLT 360
           +NYGEKVLIQFEDFANHNAF+LL +Y ++HLVFNDDIQGTASVV+AGL++ALKL+GG+L 
Sbjct: 352 QNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGLISALKLVGGSLA 411

Query: 361 DHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFK 420
           DH FLFLGAGEAGTGIAELIALEISKQT AP+EETR+KIWLVDSKGLIVRSR +SLQHFK
Sbjct: 412 DHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGLIVRSRLDSLQHFK 471

Query: 421 KPWAHDHESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTS 480
           KPWAHDHE +   L AV+AIKPTVLIG+SG G+TFTKEVVEAM+S NEKP+ILALSNPTS
Sbjct: 472 KPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNEKPIILALSNPTS 531

Query: 481 QSECTAEEAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIR 540
           QSECTAE+AYTWS+GR I+ASGSPF PVEY+GKV+V GQ+NNAYIFPGFGLGLI+SG IR
Sbjct: 532 QSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAYIFPGFGLGLIISGAIR 591

Query: 541 VHDDMLLAASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPR 600
           VHD+MLLAASEALA +V+QE++D GLIYPPFTNIRKISA+IAAKVAAKAYELGLASRLP+
Sbjct: 592 VHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAKAYELGLASRLPQ 646

Query: 601 PKDLVKFAESCMYSPRYRNY 614
           P++LV +AESCMYSP+YRNY
Sbjct: 652 PENLVAYAESCMYSPKYRNY 646

[Close]

BLAST of MS001196 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 8.6e-273
Identity = 469/615 (76.26%), Postives = 533/615 (86.67%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSV--HMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPI 60
           ME  +K      SV     +  +    E+ YGE  ATEDQL+TPWTVSVASGYSLLRDP 
Sbjct: 1   MESTLKDIRDGASVLDLDPKATVGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDPR 60

Query: 61  YNKGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNE 120
           +NKGLAF++KERDAHYL GLLPP +  QELQER++M+++RQY VPL +Y+AMMDLQERNE
Sbjct: 61  HNKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSC 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSI+RRPQGL+ISLKEK             
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEK------------- 180

Query: 181 IGSELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240
                       GKILEVLKNWPER IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT
Sbjct: 181 ------------GKILEVLKNWPERRIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240

Query: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGE 300
           ALGG+RPSACLPITIDVGTNNE+LL +EFYIGL+ +RA G+EY E L EFM  VK+NYGE
Sbjct: 241 ALGGVRPSACLPITIDVGTNNEKLLANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGE 300

Query: 301 KVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFL 360
           KVLIQFEDFANHNAF+LL++Y ++HL FNDDIQGTASVV+AG+++AL+L+GGTL DH FL
Sbjct: 301 KVLIQFEDFANHNAFDLLAKYGTTHLAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFL 360

Query: 361 FLGAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAH 420
           FLGAGEAGTGIAELIALE+SKQTK P+EETR+KIWLVDSKGLIV SR +SLQ FKKPWAH
Sbjct: 361 FLGAGEAGTGIAELIALEMSKQTKCPIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAH 420

Query: 421 DHESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECT 480
           +HE +KDLL AV+ IKPTVLIG+SGVGK FTKEV+EAMAS NEKPLILALSNPTSQSECT
Sbjct: 421 EHEPVKDLLDAVKVIKPTVLIGSSGVGKAFTKEVIEAMASCNEKPLILALSNPTSQSECT 480

Query: 481 AEEAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDM 540
           AEEAYTW++GRAI+ASGSPFDPVEY+GK FVPGQANNAYIFPG G+GL++SG IRVHD+M
Sbjct: 481 AEEAYTWTQGRAIFASGSPFDPVEYNGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEM 540

Query: 541 LLAASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLV 600
           LLAASEALA +V+QEN+DKGLIYPPF+NIRKISA+IAA VAAKAYELGLA+RLP+P++LV
Sbjct: 541 LLAASEALARQVTQENFDKGLIYPPFSNIRKISAHIAANVAAKAYELGLATRLPQPENLV 590

Query: 601 KFAESCMYSPRYRNY 614
           K+AESCMYSP YR+Y
Sbjct: 601 KYAESCMYSPVYRSY 590

[Close]

BLAST of MS001196 vs. ExPASy Swiss-Prot
Match: Q9LYG3 (NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 8.1e-271
Identity = 467/611 (76.43%), Postives = 522/611 (85.43%), Query Frame = 0


Query: 3   GAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKG 62
           G+    +    V   R  +     + YGE +AT DQL+TPW  SVASGY+L+RDP YNKG
Sbjct: 2   GSTPTDLPGEDVADNRSGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKG 61

Query: 63  LAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLFY 122
           LAF++KERDAHYL GLLPP IL Q++QER++MHNLRQY VPL RY+A+MDLQERNERLFY
Sbjct: 62  LAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFY 121

Query: 123 KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGSE 182
           KLLIDNVEELLPVVYTPTVGEACQKYGSI+R+PQGL+ISL EK                 
Sbjct: 122 KLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEK----------------- 181

Query: 183 LIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 242
                   GKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG
Sbjct: 182 --------GKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 241

Query: 243 IRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLI 302
           IRPSACLPITIDVGTNNE+LLNDEFYIGL+ +RA GQEY E L EFM AVK+NYGEKVL+
Sbjct: 242 IRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCAVKQNYGEKVLV 301

Query: 303 QFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGA 362
           QFEDFANHNAF+LLS+YS SHLVFNDDIQGTASVV+AGL+AA K++G  L DHTFLFLGA
Sbjct: 302 QFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLADHTFLFLGA 361

Query: 363 GEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHES 422
           GEAGTGIAELIAL+ISK+T AP+ ETR+KIWLVDSKGLIV SR ESLQHFK+PWAH+H+ 
Sbjct: 362 GEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQHFKQPWAHEHKP 421

Query: 423 IKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEA 482
           +KDL+GAV AIKPTVLIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNPTSQ+ECTAE+A
Sbjct: 422 VKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNPTSQAECTAEQA 481

Query: 483 YTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAA 542
           YTW+KGRAI+ SGSPFDPV YDGK ++PGQANN YIFPG GLGLIMSG IRV DDMLLAA
Sbjct: 482 YTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAA 541

Query: 543 SEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAE 602
           SEALAA+V++E+Y  GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS LPR KDLVKFAE
Sbjct: 542 SEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNLPRAKDLVKFAE 587

Query: 603 SCMYSPRYRNY 614
           S MYSP YRNY
Sbjct: 602 SSMYSPVYRNY 587

[Close]

BLAST of MS001196 vs. ExPASy TrEMBL
Match: A0A6J1BSP9 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111004998 PE=3 SV=1)

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 588/614 (95.77%), Postives = 588/614 (95.77%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 21  MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 80

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL
Sbjct: 81  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 140

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK               
Sbjct: 141 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK--------------- 200

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 201 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 260

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 261 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 320

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL
Sbjct: 321 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 380

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH
Sbjct: 381 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 440

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAM SLNEKPLILALSNPTSQSECTAE
Sbjct: 441 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMESLNEKPLILALSNPTSQSECTAE 500

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 501 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 560

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 561 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 609

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSPRYRNYL
Sbjct: 621 AESCMYSPRYRNYL 609

[Close]

BLAST of MS001196 vs. ExPASy TrEMBL
Match: A0A6J1KT37 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111496169 PE=3 SV=1)

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 568/614 (92.51%), Postives = 585/614 (95.28%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MEGAMKKTMSNGSVHME EEIDNK+EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 18  MEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 77

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAF+EKERDAHYLRGLLPPA+L+QELQERRMMHNLR Y+VPLHRYIAMMDLQERNERL
Sbjct: 78  KGLAFTEKERDAHYLRGLLPPALLNQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 137

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK               
Sbjct: 138 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK--------------- 197

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 198 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 257

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 258 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 317

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLL+ALKL+GGTL DHTFLFL
Sbjct: 318 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFL 377

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALE+SKQT+APVEETR+KIWLVDSKGLIVRSRFESLQHFKKPWAHDH
Sbjct: 378 GAGEAGTGIAELIALEVSKQTQAPVEETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 437

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE
Sbjct: 438 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 497

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWS+GRAI+ASGSPFDPVEYDGKV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 498 EAYTWSQGRAIFASGSPFDPVEYDGKVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 557

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAA+VS+ENY+KGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 558 AASEALAAQVSEENYEKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 606

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSP YR+YL
Sbjct: 618 AESCMYSPGYRSYL 606

[Close]

BLAST of MS001196 vs. ExPASy TrEMBL
Match: A0A6J1GJD0 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111454390 PE=3 SV=1)

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 568/614 (92.51%), Postives = 585/614 (95.28%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MEGAMKKTMSNGSVHME EEIDNK+EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 18  MEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 77

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAF+EKERDAHYLRGLLPPA+L+QELQERRMMHNLR Y+VPLHRYIAMMDLQERNERL
Sbjct: 78  KGLAFTEKERDAHYLRGLLPPALLNQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 137

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK               
Sbjct: 138 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK--------------- 197

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 198 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 257

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 258 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 317

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLL+ALKL+GGTL DHTFLFL
Sbjct: 318 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFL 377

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALE+SKQT+APVEETR+KIWLVDSKGLIVRSRFESLQHFKKPWAHDH
Sbjct: 378 GAGEAGTGIAELIALEVSKQTQAPVEETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 437

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE
Sbjct: 438 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 497

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWS+GRAI+ASGSPFDPVEYDGKV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 498 EAYTWSQGRAIFASGSPFDPVEYDGKVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 557

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAA+VS+ENY+KGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 558 AASEALAAQVSEENYEKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 606

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSP YR+YL
Sbjct: 618 AESCMYSPGYRSYL 606

[Close]

BLAST of MS001196 vs. ExPASy TrEMBL
Match: A0A0A0LLH9 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_2G373430 PE=3 SV=1)

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 567/614 (92.35%), Postives = 580/614 (94.46%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           ME +MKKTMSNGSVHM+ EEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPA++DQELQERRMMHNLR Y+VPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIG 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEK               
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEK--------------- 180

Query: 181 SELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240
                     GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 181 ----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 240

Query: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKV 300
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGLR KRARGQEYMELLDEFMYAVKKNYGEKV
Sbjct: 241 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKV 300

Query: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFL 360
           LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTL DHTFLFL
Sbjct: 301 LIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFL 360

Query: 361 GAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDH 420
           GAGEAGTGIAELIALEISKQT AP EETR+KIWLVDSKGLIV+SRFESLQHFKKPWAHDH
Sbjct: 361 GAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDH 420

Query: 421 ESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAE 480
           ESIKDL GAVQAIKPTVLIGTSGVGKTFTKEVVEAMAS NEKPLILALSNPTSQSECTAE
Sbjct: 421 ESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAE 480

Query: 481 EAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540
           EAYTWS+GRAI+ASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL
Sbjct: 481 EAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLL 540

Query: 541 AASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 600
           AASEALAA+VSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF
Sbjct: 541 AASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKF 589

Query: 601 AESCMYSPRYRNYL 615
           AESCMYSPRYR+YL
Sbjct: 601 AESCMYSPRYRSYL 589

[Close]

BLAST of MS001196 vs. ExPASy TrEMBL
Match: A0A5A7VH34 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003800 PE=3 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 570/615 (92.68%), Postives = 581/615 (94.47%), Query Frame = 0


Query: 1   MEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           ME +MKKTMSNGSVHME EEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAILDQEL-QERRMMHNLRQYDVPLHRYIAMMDLQERNER 120
           KGLAFSEKERDAHYLRGLLPPA+LDQEL QERRMMHNLR Y+VPLHRYIAMMDLQERNER
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPALLDQELQQERRMMHNLRNYEVPLHRYIAMMDLQERNER 120

Query: 121 LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCI 180
           LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK              
Sbjct: 121 LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEK-------------- 180

Query: 181 GSELIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTA 240
                      GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTA
Sbjct: 181 -----------GKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTA 240

Query: 241 LGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEK 300
           LGGIRPSACLPITIDVGTNNEQLLNDEFYIGLR KRARGQEYMELLDEFMYAVKKNYGEK
Sbjct: 241 LGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEK 300

Query: 301 VLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLF 360
           VLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKL+GGTL DHTFLF
Sbjct: 301 VLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLF 360

Query: 361 LGAGEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHD 420
           LGAGEAGTGIAELIALEISKQT AP+EETR+KIWLVDSKGLIV+SRFESLQHFKKPWAHD
Sbjct: 361 LGAGEAGTGIAELIALEISKQTGAPIEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHD 420

Query: 421 HESIKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTA 480
           HESIKDL GAVQAIKPTVLIGTSGVGKTFTKEVVEAMAS NEKPLILALSNPTSQSECTA
Sbjct: 421 HESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTA 480

Query: 481 EEAYTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDML 540
           EEAYTWS+GRAI+ASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDML
Sbjct: 481 EEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDML 540

Query: 541 LAASEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVK 600
           LAASEALAA+VSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVK
Sbjct: 541 LAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVK 590

Query: 601 FAESCMYSPRYRNYL 615
           FAESCMYSPRYR+YL
Sbjct: 601 FAESCMYSPRYRSYL 590

[Close]

BLAST of MS001196 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 943.7 bits (2438), Expect = 7.2e-275
Identity = 470/611 (76.92%), Postives = 524/611 (85.76%), Query Frame = 0


Query: 3   GAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKG 62
           G  +  +S+  V      +     + YGE +AT DQL+TPW  SVASGY+L+RDP YNKG
Sbjct: 2   GTNQTQISDEYVTGNSSGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKG 61

Query: 63  LAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLFY 122
           LAF++KERDAHY+ GLLPP +L Q++QER++MHNLRQY VPL RY+A+MDLQERNERLFY
Sbjct: 62  LAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFY 121

Query: 123 KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGSE 182
           KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGL+ISLKEK                 
Sbjct: 122 KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEK----------------- 181

Query: 183 LIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 242
                   GKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG
Sbjct: 182 --------GKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 241

Query: 243 IRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLI 302
           IRPSACLPITIDVGTNNE+LLN+EFYIGL+ KRA G+EY E L EFM AVK+NYGEKVL+
Sbjct: 242 IRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCAVKQNYGEKVLV 301

Query: 303 QFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGA 362
           QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +L DHTFLFLGA
Sbjct: 302 QFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLADHTFLFLGA 361

Query: 363 GEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHES 422
           GEAGTGIAELIAL+ISK+T  P++ETR+KIWLVDSKGLIV  R ESLQHFK+PWAHDH+ 
Sbjct: 362 GEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWAHDHKP 421

Query: 423 IKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEA 482
           +K+LL AV AIKPTVLIGTSGVGKTFTKEVVEAMA+LNEKPLILALSNPTSQ+ECTAEEA
Sbjct: 422 VKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNPTSQAECTAEEA 481

Query: 483 YTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAA 542
           YTW+KGRAI+ASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG IRV DDMLLAA
Sbjct: 482 YTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAA 541

Query: 543 SEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAE 602
           SEALA++V++EN+  GLIYPPF NIRKISANIAA V AK YELGLAS LPRPKDLVK AE
Sbjct: 542 SEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNLPRPKDLVKMAE 587

Query: 603 SCMYSPRYRNY 614
           SCMYSP YRN+
Sbjct: 602 SCMYSPVYRNF 587

[Close]

BLAST of MS001196 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 934.1 bits (2413), Expect = 5.7e-272
Identity = 467/611 (76.43%), Postives = 522/611 (85.43%), Query Frame = 0


Query: 3   GAMKKTMSNGSVHMEREEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKG 62
           G+    +    V   R  +     + YGE +AT DQL+TPW  SVASGY+L+RDP YNKG
Sbjct: 2   GSTPTDLPGEDVADNRSGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKG 61

Query: 63  LAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLFY 122
           LAF++KERDAHYL GLLPP IL Q++QER++MHNLRQY VPL RY+A+MDLQERNERLFY
Sbjct: 62  LAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFY 121

Query: 123 KLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGSE 182
           KLLIDNVEELLPVVYTPTVGEACQKYGSI+R+PQGL+ISL EK                 
Sbjct: 122 KLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEK----------------- 181

Query: 183 LIIIFLFRGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 242
                   GKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG
Sbjct: 182 --------GKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG 241

Query: 243 IRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLI 302
           IRPSACLPITIDVGTNNE+LLNDEFYIGL+ +RA GQEY E L EFM AVK+NYGEKVL+
Sbjct: 242 IRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCAVKQNYGEKVLV 301

Query: 303 QFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGA 362
           QFEDFANHNAF+LLS+YS SHLVFNDDIQGTASVV+AGL+AA K++G  L DHTFLFLGA
Sbjct: 302 QFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLADHTFLFLGA 361

Query: 363 GEAGTGIAELIALEISKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHES 422
           GEAGTGIAELIAL+ISK+T AP+ ETR+KIWLVDSKGLIV SR ESLQHFK+PWAH+H+ 
Sbjct: 362 GEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQHFKQPWAHEHKP 421

Query: 423 IKDLLGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEA 482
           +KDL+GAV AIKPTVLIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNPTSQ+ECTAE+A
Sbjct: 422 VKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNPTSQAECTAEQA 481

Query: 483 YTWSKGRAIYASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAA 542
           YTW+KGRAI+ SGSPFDPV YDGK ++PGQANN YIFPG GLGLIMSG IRV DDMLLAA
Sbjct: 482 YTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAA 541

Query: 543 SEALAAEVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAE 602
           SEALAA+V++E+Y  GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS LPR KDLVKFAE
Sbjct: 542 SEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNLPRAKDLVKFAE 587

Query: 603 SCMYSPRYRNY 614
           S MYSP YRNY
Sbjct: 602 SSMYSPVYRNY 587

[Close]

BLAST of MS001196 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 922.2 bits (2382), Expect = 2.3e-268
Identity = 453/596 (76.01%), Postives = 521/596 (87.42%), Query Frame = 0


Query: 18  REEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRG 77
           +  +    ++ YGE  ATED  ITPW++SVASGY+LLRDP +NKGLAFS +ERDAHYLRG
Sbjct: 75  KSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRG 134

Query: 78  LLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVY 137
           LLPP ++ Q+LQ +++MH LRQY VPL +Y+AMMDLQE NERLFYKLLID+VEELLPV+Y
Sbjct: 135 LLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIY 194

Query: 138 TPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGSELIIIFLFRGKILEVL 197
           TPTVGEACQKYGSI+ RPQGLFISLKEK                         GKI EVL
Sbjct: 195 TPTVGEACQKYGSIFLRPQGLFISLKEK-------------------------GKIHEVL 254

Query: 198 KNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGT 257
           +NWPE+NIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLP+TIDVGT
Sbjct: 255 RNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGT 314

Query: 258 NNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLS 317
           NNE+LLNDEFYIGLR +RA G+EY EL+ EFM AVK+NYGEKV+IQFEDFANHNAF+LL+
Sbjct: 315 NNEKLLNDEFYIGLRQRRATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLA 374

Query: 318 RYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGAGEAGTGIAELIALEI 377
           +Y ++HLVFNDDIQGTASVV+AGL+AAL+ +GG+L+DH FLFLGAGEAGTGIAELIALEI
Sbjct: 375 KYGTTHLVFNDDIQGTASVVLAGLIAALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEI 434

Query: 378 SKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTV 437
           SK++  P+EE R+ IWLVDSKGLIV SR ES+QHFKKPWAHDHE I++L+ AV+AIKPTV
Sbjct: 435 SKKSHIPLEEARKNIWLVDSKGLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTV 494

Query: 438 LIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIYASGSP 497
           LIGTSGVG+TFT++VVE MA LNEKP+IL+LSNPTSQSECTAEEAYTWS+GRAI+ASGSP
Sbjct: 495 LIGTSGVGQTFTQDVVETMAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSP 554

Query: 498 FDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAEVSQENYDK 557
           F PVEY+GK FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALA E+ +E+Y+K
Sbjct: 555 FAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEK 614

Query: 558 GLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRNY 614
           G+IYPPF NIRKISA IAAKVAAKAYELGLA+RLP+PK+L + AES MYSP YR+Y
Sbjct: 615 GMIYPPFRNIRKISARIAAKVAAKAYELGLATRLPQPKELEQCAESSMYSPSYRSY 645

[Close]

BLAST of MS001196 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 920.2 bits (2377), Expect = 8.6e-268
Identity = 453/596 (76.01%), Postives = 521/596 (87.42%), Query Frame = 0


Query: 18  REEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRG 77
           +  +D    + YGE +AT +  ITPW++SV+SGYSLLRDP YNKGLAF+EKERD HYLRG
Sbjct: 10  KSSVDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRG 69

Query: 78  LLPPAILDQELQERRMMHNLRQYDVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVY 137
           LLPP +LDQ+LQE+R+++N+RQY  PL +Y+A+ +LQERNERLFYKLLIDNVEELLP+VY
Sbjct: 70  LLPPVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVY 129

Query: 138 TPTVGEACQKYGSIYRRPQGLFISLKEKYAIRKALIMFVSCIGSELIIIFLFRGKILEVL 197
           TPTVGEACQK+GSI+RRPQGLFISLK+K                         GKIL+VL
Sbjct: 130 TPTVGEACQKFGSIFRRPQGLFISLKDK-------------------------GKILDVL 189

Query: 198 KNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGT 257
           KNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKL+LY+ALGG+RPSACLP+TIDVGT
Sbjct: 190 KNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGT 249

Query: 258 NNEQLLNDEFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLS 317
           NNE+LLNDEFYIGLR KRA GQEY ELL+EFM AVK+NYGEKVLIQFEDFANHNAFELL+
Sbjct: 250 NNEKLLNDEFYIGLRQKRATGQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLA 309

Query: 318 RYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGAGEAGTGIAELIALEI 377
           +YS +HLVFNDDIQGTASVV+AGL++A KL    L +HTFLFLGAGEAGTGIAELIAL +
Sbjct: 310 KYSDTHLVFNDDIQGTASVVLAGLVSAQKLTNSPLAEHTFLFLGAGEAGTGIAELIALYM 369

Query: 378 SKQTKAPVEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTV 437
           SKQ  A VEE+R+KIWLVDSKGLIV SR +SLQ FKKPWAH+HE +KDLLGA++AIKPTV
Sbjct: 370 SKQMNASVEESRKKIWLVDSKGLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTV 429

Query: 438 LIGTSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIYASGSP 497
           LIG+SGVG++FTKEV+EAM+S+NE+PLI+ALSNPT+QSECTAEEAYTWSKGRAI+ASGSP
Sbjct: 430 LIGSSGVGRSFTKEVIEAMSSINERPLIMALSNPTTQSECTAEEAYTWSKGRAIFASGSP 489

Query: 498 FDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAEVSQENYDK 557
           FDPVEY+GKVFV  QANNAYIFPGFGLGL++SG IRVHDDMLLAA+EALA +VS+ENY+K
Sbjct: 490 FDPVEYEGKVFVSTQANNAYIFPGFGLGLVISGAIRVHDDMLLAAAEALAGQVSKENYEK 549

Query: 558 GLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRNY 614
           G+IYP F++IRKISA IAA VA KAYELGLA RLPRPKD+VK AES MYSP YR Y
Sbjct: 550 GMIYPSFSSIRKISAQIAANVATKAYELGLAGRLPRPKDIVKCAESSMYSPTYRLY 580

[Close]

BLAST of MS001196 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-117
Identity = 242/603 (40.13%), Postives = 357/603 (59.20%), Query Frame = 0


Query: 44  TVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAILDQELQERRMMHNLRQYDV- 103
           T+    G  +L DP +NKG AF+  ER+   LRGLLPP ++D E Q  R M +L++ +  
Sbjct: 40  TIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQ 99

Query: 104 ---------PLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 163
                     L ++  +  L +RNE ++YK+LI+N+EE  P+VYTPTVG  CQ Y  ++R
Sbjct: 100 ARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159

Query: 164 RPQGLFISLKEKYAIRKALIMFVSCIGSELIIIFLFRGKILEVLKNWPERNIQVIVVTDG 223
           RP+G++ S ++                         RG+++ ++ NWP   + +IVVTDG
Sbjct: 160 RPRGMYFSAED-------------------------RGEMMSMVYNWPAEQVDMIVVTDG 219

Query: 224 ERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRH 283
            RILGLGDLG  G+GI VGKL LY A  GI P   LP+ IDVGTNNE+L ND  Y+GL+ 
Sbjct: 220 SRILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQ 279

Query: 284 KRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGT 343
           +R    +Y++++DEFM AV   +   V++QFEDF +  AF+LL RY  ++ +FNDD+QGT
Sbjct: 280 RRLEDDDYIDVIDEFMEAVYTRW-PHVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGT 339

Query: 344 ASVVVAGLLAALKLIGGTLTDH---TFLFLGAGEAGTGIAELIALEISK---QTKAPVEE 403
           A V +AGLL A++  G  + D      +  GAG AG G+       +++    T+   + 
Sbjct: 340 AGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARMLGNTETAFDS 399

Query: 404 TRRKIWLVDSKGLIVRSRFESLQHFKKPWAH-----DHESIKD---LLGAVQAIKPTVLI 463
            + + W+VD++GLI   R E++    +P+A      + + +K+   L+  V+ +KP VL+
Sbjct: 400 AQSQFWVVDAQGLITEGR-ENIDPEAQPFARKTKEMERQGLKEGATLVEVVREVKPDVLL 459

Query: 464 GTSGVGKTFTKEVVEAM-ASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIYASGSPF 523
           G S VG  F+KEV+EAM  S + +P I A+SNPT  +ECT ++A++      I+ASGSPF
Sbjct: 460 GLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSNPTKNAECTPQDAFSILGENMIFASGSPF 519

Query: 524 DPVEY-DGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAEVSQENYDK 583
             VE+ +G V    Q NN Y+FPG GLG ++SG   V D ML AASE LAA +S+E   +
Sbjct: 520 KNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAASECLAAYMSEEEVLE 579

Query: 584 GLIYPPFTNIRKISANIAAKVAAKAYELGL--------ASRLPR--PKDLVKFAESCMYS 611
           G+IYPP + IR I+  IAA V  +A E  L        A  + +   + L+++ E+ M++
Sbjct: 580 GIIYPPISRIRDITKRIAAAVIKEAIEEDLVEGYREMDAREIQKLDEEGLMEYVENNMWN 615

The following BLAST results are available for this feature:

BLAST of MS001196 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132027.1	0.0e+00	95.77	NADP-dependent malic enzyme [Momordica charantia]	[more]
XP_022951634.1	0.0e+00	92.51	NADP-dependent malic enzyme [Cucurbita moschata] >XP_023002278.1 NADP-dependent ...	[more]
KAG7020293.1	0.0e+00	92.35	NADP-dependent malic enzyme [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6585377.1	0.0e+00	92.18	NADP-dependent malic enzyme, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_011649727.1	0.0e+00	92.35	NADP-dependent malic enzyme isoform X1 [Cucumis sativus] >KGN63047.2 hypothetica...	[more]

BLAST of MS001196 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P12628	6.8e-286	80.88	NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1	[more]
Q9XGZ0	1.0e-273	76.92	NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME3 PE=1 S...	[more]
P36444	3.0e-273	74.84	NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ...	[more]
P51615	8.6e-273	76.26	NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
Q9LYG3	8.1e-271	76.43	NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 S...	[more]

BLAST of MS001196 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSP9	0.0e+00	95.77	Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111004998 PE=3 SV=1	[more]
A0A6J1KT37	0.0e+00	92.51	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111496169 PE=3 SV=1	[more]
A0A6J1GJD0	0.0e+00	92.51	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111454390 PE=3 SV=1	[more]
A0A0A0LLH9	0.0e+00	92.35	Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_2G373430 PE=3 SV=1	[more]
A0A5A7VH34	0.0e+00	92.68	Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003800 P...	[more]

BLAST of MS001196 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25880.1	7.2e-275	76.92	NADP-malic enzyme 3	[more]
AT5G11670.1	5.7e-272	76.43	NADP-malic enzyme 2	[more]
AT1G79750.1	2.3e-268	76.01	NADP-malic enzyme 4	[more]
AT2G19900.1	8.6e-268	76.01	NADP-malic enzyme 1	[more]
AT2G13560.1	1.6e-117	40.13	NAD-dependent malic enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001891	Malic oxidoreductase	PRINTS	PR00072	MALOXRDTASE	coord: 460..476 score: 67.06 coord: 120..144 score: 69.8 coord: 357..373 score: 71.32
IPR001891	Malic oxidoreductase	PIRSF	PIRSF000106	ME	coord: 10..186 e-value: 5.5E-42 score: 142.0 coord: 188..614 e-value: 1.3E-184 score: 612.9
IPR012301	Malic enzyme, N-terminal domain	SMART	SM01274	malic_2	coord: 114..320 e-value: 8.9E-96 score: 334.2
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 190..319 e-value: 8.5E-58 score: 195.1 coord: 114..173 e-value: 1.5E-15 score: 57.5
IPR012302	Malic enzyme, NAD-binding	SMART	SM00919	Malic_M_2	coord: 330..583 e-value: 8.1E-113 score: 390.8
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 330..582 e-value: 1.5E-95 score: 319.3
None	No IPR available	GENE3D	3.40.50.720		coord: 328..614 e-value: 1.4E-120 score: 404.2
None	No IPR available	PANTHER	PTHR23406:SF65	MALIC ENZYME	coord: 5..171 coord: 184..613
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 5..171 coord: 184..613
None	No IPR available	CDD	cd05312	NAD_bind_1_malic_enz	coord: 330..608 e-value: 9.71372E-144 score: 416.179
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 27..329
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	3.40.50.10380		coord: 48..327 e-value: 6.0E-122 score: 407.7
IPR015884	Malic enzyme, conserved site	PROSITE	PS00331	MALIC_ENZYMES	coord: 326..342
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 330..613

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001196.1	MS001196.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0004470	malic enzyme activity
molecular_function	GO:0051287	NAD binding