MC07g0841 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0841
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionenoyl-CoA hydratase/isomerase A
LocationMC07: 15945302 .. 15946991 (+)
RNA-Seq ExpressionMC07g0841
SyntenyMC07g0841
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAATGTTAAAATCCAGTAACAAACACAAATCAATACGAATTTATTTTATTGAATTGTTTGTTTTCAAATTTCTATTTTTAGATTTCTGTGTCAAGGCTGTATTTACTGGAGAGTTCCTATCTCTGAGTTTATCCACTTTCTGCACAATTGCAATTGCGCCACTGGAGGCTAACGTCAGATATGAGATTTCTACGTTTCTCGATGCCATCTCTTCACTGAACCGCGCCATATCGCACCCAATTCCAGTACAATAACCCATTTTAATTCTGCTTTGCTTCACGATTCTTTGGCCGGAATGGATCCTTCTTCGCCGGAGAAGCTCATACTCGTTGACCGAGAGCCAAACGGGATCGCATTTGTTACTATTAACCGTCCCAAGTCGCTGAATTCGTTAACCAGACCCATGATTGTAGATTTGGCCCACGCGATTAAGAGCTTGGACCGCGACCAGTCGGTGCGGGTCATCGTGCTCTCGGGATCAGGGCGAGCCTTCTGTTCCGGTGTAGACCTTAGTGCGGCGGAGGAGGTGTTCAAGGGGGATGTGAAGGACGTTGAATCCGATCCGGTTCTGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTGCGGGGTTCGCGATCACCGCTGGGTTCGAGATCGCTCTGGCTTGCGACATACTTATCGCTGCCAAAGGGGCTAAGTTCATCGACACTCATGCTAGGTTTGTTTGTTATTTACCATTTCTGTAAAGCGAATTAGGATCAAGAATAATTAATTTTCTGAAATCTTTTGGGAAACGCTTTTCTATTGAAATGCCCAATTAAGAACGTGTCATATCTGGGAAAACTTGGTTGCAGATTTGGGATATTTCCTTCGTGGGGATTGTCTCAGAAGCTCTCACGGATCATTGGAGTTAACAAAGCTCGTGAAGTATCCCTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAGGGGTTTGGTGAATCACGTGGTGGAAGGAGGTGAGTTGTTGAAGAAAGCTCGAGAAGTGGCGGAGGCCATCTTAAAGAACAATCAGGACATGGTGTTGAGGTACAAGTCGGTCATTAACGATGGCCTTCAGCTGGACCTTGGCCACGCTCTTGCGCTGGAAAAGGTACTTGCCCAACCCCTTGAAATTTGCTAGAAAGATACTGATTATGAATGATACTAAATTCAATTTTATCCCCAAATTGATGTCAGTGATTGATTGGAGATAATTCATAAGTAGTTTTGTTTTCAGATGACAGAGCTTGTGTAGTAGAGTAGCATTGTCCAATCGACTGTGTGTTACTTTGATGCAGGAGAGAGGGCATGCGTATTACAATGGAATGACAAAAGAGCAGTTCAAGAAAATGCAGGAATTTATAGCTGGTCGGAGCTCCAAGAAACCTTCAAAGTTATAGTCTAAACTAAACGACGAGTTTCCTGTCTCTGTTTCTACAGTTGTGCCAATAATATGGCAAAAACTTGCCTCCTCACCGGTCAGTAGTTTCCCTTCCTCCTTAAATTTGTTTATATATGTAATATGTAATTTGATTTCAGTGGATCAAAAGCATCTGTGCAATTGATATACTTATAGAAATAGATAAAGTAGAAAATAGACAGGTGTTTGGGCAATAAAGTTGGATTTTTGGTGGAATATGGTTTCTTATCTCATGTCATGTGCTGGAGTTTGAGA

mRNA sequence

TTTAATGTTAAAATCCAGTAACAAACACAAATCAATACGAATTTATTTTATTGAATTGTTTGTTTTCAAATTTCTATTTTTAGATTTCTGTGTCAAGGCTGTATTTACTGGAGAGTTCCTATCTCTGAGTTTATCCACTTTCTGCACAATTGCAATTGCGCCACTGGAGGCTAACGTCAGATATGAGATTTCTACGTTTCTCGATGCCATCTCTTCACTGAACCGCGCCATATCGCACCCAATTCCAGTACAATAACCCATTTTAATTCTGCTTTGCTTCACGATTCTTTGGCCGGAATGGATCCTTCTTCGCCGGAGAAGCTCATACTCGTTGACCGAGAGCCAAACGGGATCGCATTTGTTACTATTAACCGTCCCAAGTCGCTGAATTCGTTAACCAGACCCATGATTGTAGATTTGGCCCACGCGATTAAGAGCTTGGACCGCGACCAGTCGGTGCGGGTCATCGTGCTCTCGGGATCAGGGCGAGCCTTCTGTTCCGGTGTAGACCTTAGTGCGGCGGAGGAGGTGTTCAAGGGGGATGTGAAGGACGTTGAATCCGATCCGGTTCTGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTGCGGGGTTCGCGATCACCGCTGGGTTCGAGATCGCTCTGGCTTGCGACATACTTATCGCTGCCAAAGGGGCTAAGTTCATCGACACTCATGCTAGATTTGGGATATTTCCTTCGTGGGGATTGTCTCAGAAGCTCTCACGGATCATTGGAGTTAACAAAGCTCGTGAAGTATCCCTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAGGGGTTTGGTGAATCACGTGGTGGAAGGAGGTGAGTTGTTGAAGAAAGCTCGAGAAGTGGCGGAGGCCATCTTAAAGAACAATCAGGACATGGTGTTGAGGTACAAGTCGGTCATTAACGATGGCCTTCAGCTGGACCTTGGCCACGCTCTTGCGCTGGAAAAGGAGAGAGGGCATGCGTATTACAATGGAATGACAAAAGAGCAGTTCAAGAAAATGCAGGAATTTATAGCTGGTCGGAGCTCCAAGAAACCTTCAAAGTTATAGTCTAAACTAAACGACGAGTTTCCTGTCTCTGTTTCTACAGTTGTGCCAATAATATGGCAAAAACTTGCCTCCTCACCGGTCAGTAGTTTCCCTTCCTCCTTAAATTTGTTTATATATGTAATATGTAATTTGATTTCAGTGGATCAAAAGCATCTGTGCAATTGATATACTTATAGAAATAGATAAAGTAGAAAATAGACAGGTGTTTGGGCAATAAAGTTGGATTTTTGGTGGAATATGGTTTCTTATCTCATGTCATGTGCTGGAGTTTGAGA

Coding sequence (CDS)

ATGGATCCTTCTTCGCCGGAGAAGCTCATACTCGTTGACCGAGAGCCAAACGGGATCGCATTTGTTACTATTAACCGTCCCAAGTCGCTGAATTCGTTAACCAGACCCATGATTGTAGATTTGGCCCACGCGATTAAGAGCTTGGACCGCGACCAGTCGGTGCGGGTCATCGTGCTCTCGGGATCAGGGCGAGCCTTCTGTTCCGGTGTAGACCTTAGTGCGGCGGAGGAGGTGTTCAAGGGGGATGTGAAGGACGTTGAATCCGATCCGGTTCTGCAAATGGAACGCTGCCGGAAGCCGATCATCGGAGCGATTGCGGGGTTCGCGATCACCGCTGGGTTCGAGATCGCTCTGGCTTGCGACATACTTATCGCTGCCAAAGGGGCTAAGTTCATCGACACTCATGCTAGATTTGGGATATTTCCTTCGTGGGGATTGTCTCAGAAGCTCTCACGGATCATTGGAGTTAACAAAGCTCGTGAAGTATCCCTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAGGGGTTTGGTGAATCACGTGGTGGAAGGAGGTGAGTTGTTGAAGAAAGCTCGAGAAGTGGCGGAGGCCATCTTAAAGAACAATCAGGACATGGTGTTGAGGTACAAGTCGGTCATTAACGATGGCCTTCAGCTGGACCTTGGCCACGCTCTTGCGCTGGAAAAGGAGAGAGGGCATGCGTATTACAATGGAATGACAAAAGAGCAGTTCAAGAAAATGCAGGAATTTATAGCTGGTCGGAGCTCCAAGAAACCTTCAAAGTTATAG

Protein sequence

MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLVNHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNGMTKEQFKKMQEFIAGRSSKKPSKL
Homology
BLAST of MC07g0841 vs. ExPASy Swiss-Prot
Match: Q6NL24 (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECHIA PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 2.8e-111
Identity = 203/262 (77.48%), Postives = 230/262 (87.79%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD +  E LI V +E  GIA +TINRPKSLNSLTR M+VDLA A K +D D+SV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGR+FCSGVDL+AAE VFKGDVKD E+DPV+QMER RKPIIGAI GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DIL+A++GAKF+DTHARFGIFPSWGLSQKLSRIIG NKAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVVE GE LKKARE+AEAI+KN Q MVLR KSVINDGL+LDLGHAL LEKER HAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of MC07g0841 vs. ExPASy Swiss-Prot
Match: P52046 (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=crt PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 8.6e-36
Identity = 92/251 (36.65%), Postives = 149/251 (59.36%), Query Frame = 0

Query: 10  ILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSG-RAFCS 69
           +++++E   +A VTINRPK+LN+L    + ++ + I  ++ D  V  ++L+G+G ++F +
Sbjct: 6   VILEKE-GKVAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVA 65

Query: 70  GVDLSAAEEV--FKGDVKDVESDPVL-QMERCRKPIIGAIAGFAITAGFEIALACDILIA 129
           G D+S  +E+   +G    +  + V  ++E   KP+I A+ GFA+  G EIA++CDI IA
Sbjct: 66  GADISEMKEMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIA 125

Query: 130 AKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLVNHVVE 189
           +  A+F       GI P +G +Q+LSR++G+  A+++  TA  + A+EA R GLVN VVE
Sbjct: 126 SSNARFGQPEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVE 185

Query: 190 GGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNGMTKEQ 249
             EL+  A+E+A  I+ N    V   K  IN G+Q D+  ALA E E     ++  T++Q
Sbjct: 186 PSELMNTAKEIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS--TEDQ 245

Query: 250 FKKMQEFIAGR 257
              M  FI  R
Sbjct: 246 KDAMTAFIEKR 253

BLAST of MC07g0841 vs. ExPASy Swiss-Prot
Match: Q1ZXF1 (Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=echs1 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.0e-33
Identity = 89/242 (36.78%), Postives = 137/242 (56.61%), Query Frame = 0

Query: 19  IAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSGRAFCSGVDLSAAEEV 78
           +  VT+NRPKSLN+L+  +I ++  A+K    D+ V  I+++GS +AF +G D+   E+V
Sbjct: 34  VGLVTLNRPKSLNALSDGLISEINSAVKLFQEDKDVGSIIITGSEKAFAAGADIKEMEKV 93

Query: 79  FKGDVKDVESDPVLQ---MERCRKPIIGAIAGFAITAGFEIALACDILIAAKGAKFIDTH 138
              D     +D + Q   + + RKPII A+ G+A+  G E+A+ CDI+IA + A F    
Sbjct: 94  TLPDA--YNNDLLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGEKAVFGQPE 153

Query: 139 ARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLVNHVVEGGELLKKARE 198
            + G  P  G +Q+L R IG +KA E+ LT   LTA EAE+ GLV+ VV   ELL +A +
Sbjct: 154 IKLGTIPGCGGTQRLIRAIGKSKAMELVLTGNNLTAVEAEKAGLVSKVVPVEELLTEATK 213

Query: 199 VAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNGMTKEQFKKMQEFIAG 258
           +AE I   +Q  V   K  +N   +L L   +  E+   H+ +   T +Q + M  F+  
Sbjct: 214 MAEKIASYSQLTVAMAKEAVNASYELTLQEGIRFERRMFHSTFG--THDQKEGMNAFVEK 271

BLAST of MC07g0841 vs. ExPASy Swiss-Prot
Match: P64017 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA8 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 9.8e-32
Identity = 83/249 (33.33%), Postives = 134/249 (53.82%), Query Frame = 0

Query: 10  ILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSGRAFCSG 69
           ILV+R+   +  +T+NRP++LN+L   ++ ++  A   LD D  +  I+++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLSAAEEVFKGDVKDVESDPVL-QMERCRKPIIGAIAGFAITAGFEIALACDILIAAKG 129
            D+    ++   D    +      ++   R P I A+AG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLVNHVVEGGE 189
           AKF     + G+ P  G SQ+L+R IG  KA ++ LT   + A EAER GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNGMTKEQFKK 249
           LL +AR  A  I + +       K  +N   +  L   L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

BLAST of MC07g0841 vs. ExPASy Swiss-Prot
Match: O07137 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium leprae (strain TN) OX=272631 GN=echA8 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 9.8e-32
Identity = 86/249 (34.54%), Postives = 136/249 (54.62%), Query Frame = 0

Query: 10  ILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSGRAFCSG 69
           ILVD     +  +T+NRP++LN+L   M+ ++ +A K LD D  V  I+++GS + F +G
Sbjct: 6   ILVDGYQR-VGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKVFAAG 65

Query: 70  VDLSAAEEVFKGDVKDVESDPVL-QMERCRKPIIGAIAGFAITAGFEIALACDILIAAKG 129
            D+     +   D  D +      ++   R P+I A+AG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMASLTFTDAFDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLVNHVVEGGE 189
           AKF     + G+ P  G SQ+L+R IG  KA ++ LT   + A EAER GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVLADD 185

Query: 190 LLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNGMTKEQFKK 249
           LL +A+ VA  I + ++      K  +N   +  L   L  E+   H+ +  +T +Q + 
Sbjct: 186 LLPEAKAVATTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTF--VTDDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

BLAST of MC07g0841 vs. NCBI nr
Match: XP_022138775.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Momordica charantia])

HSP 1 Score: 509 bits (1312), Expect = 8.90e-182
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS
Sbjct: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG
Sbjct: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. NCBI nr
Match: XP_023548460.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477 bits (1227), Expect = 8.05e-169
Identity = 243/264 (92.05%), Postives = 257/264 (97.35%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SSPEKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A KSLDRD+SVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGV+KAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKERGHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. NCBI nr
Match: XP_022959404.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 475 bits (1222), Expect = 4.65e-168
Identity = 241/264 (91.29%), Postives = 256/264 (96.97%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SSPEKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A K+LDRD+SVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. NCBI nr
Match: KAG6575117.1 (putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 473 bits (1217), Expect = 2.69e-167
Identity = 241/264 (91.29%), Postives = 255/264 (96.59%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SSPEKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A K+LDRD+SVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKER HAYY G
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYTG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. NCBI nr
Match: XP_023006283.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima])

HSP 1 Score: 472 bits (1215), Expect = 5.42e-167
Identity = 241/264 (91.29%), Postives = 255/264 (96.59%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SS EKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A KSLDRD+SVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. ExPASy TrEMBL
Match: A0A6J1CAG2 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009856 PE=4 SV=1)

HSP 1 Score: 509 bits (1312), Expect = 4.31e-182
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS
Sbjct: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG
Sbjct: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. ExPASy TrEMBL
Match: A0A6J1H7Z0 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111460389 PE=4 SV=1)

HSP 1 Score: 475 bits (1222), Expect = 2.25e-168
Identity = 241/264 (91.29%), Postives = 256/264 (96.97%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SSPEKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A K+LDRD+SVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. ExPASy TrEMBL
Match: A0A6J1L4H0 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 472 bits (1215), Expect = 2.63e-167
Identity = 241/264 (91.29%), Postives = 255/264 (96.59%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SS EKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A KSLDRD+SVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. ExPASy TrEMBL
Match: A0A6J1L1R6 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 469 bits (1207), Expect = 4.35e-166
Identity = 240/264 (90.91%), Postives = 254/264 (96.21%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SS EKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A KSLDRD+SVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of MC07g0841 vs. ExPASy TrEMBL
Match: A0A1S3C8X4 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497788 PE=4 SV=1)

HSP 1 Score: 464 bits (1195), Expect = 2.93e-164
Identity = 239/264 (90.53%), Postives = 251/264 (95.08%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD SSPE LILV+RE NGIAF+TINRPKSLNSLTRPM++DLA A KSLDRD SVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDL+AAE+VFKGDVKDVESDPV QME CRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE+RGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           N VVEG ELLKKAREVAEAILKNNQD+VLRYKSVINDGL+LDLG ALALEKER HAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of MC07g0841 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 402.9 bits (1034), Expect = 2.0e-112
Identity = 203/262 (77.48%), Postives = 230/262 (87.79%), Query Frame = 0

Query: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60
           MD +  E LI V +E  GIA +TINRPKSLNSLTR M+VDLA A K +D D+SV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120
           GSGR+FCSGVDL+AAE VFKGDVKD E+DPV+QMER RKPIIGAI GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180
           DIL+A++GAKF+DTHARFGIFPSWGLSQKLSRIIG NKAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240
           NHVVE GE LKKARE+AEAI+KN Q MVLR KSVINDGL+LDLGHAL LEKER HAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of MC07g0841 vs. TAIR 10
Match: AT1G60550.1 (enoyl-CoA hydratase/isomerase D )

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-25
Identity = 77/214 (35.98%), Postives = 113/214 (52.80%), Query Frame = 0

Query: 18  GIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSG-RAFCSGVDLSAAE 77
           GIA +TINRP+  N+     + +L  A      D SV VI+L+G G +AFCSG D +   
Sbjct: 86  GIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQALRT 145

Query: 78  EVFKGDVKDVES----DPVLQMERCRKPIIGAIAGFAITAGFEIALACDILIAAKGAKFI 137
           +    D  DV      D  +Q+ R  KP+I  +AG+A+  G  + + CD+ IAA  A F 
Sbjct: 146 QDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAADNAIFG 205

Query: 138 DTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLVNHVVEGGELLKK 197
            T  + G F +   S  +SR++G  KARE+       TA EAE+ GL+N VV   +L K+
Sbjct: 206 QTGPKVGSFDAGYGSSIMSRLVGPKKAREMWFMTRFYTASEAEKMGLINTVVPLEDLEKE 265

Query: 198 AREVAEAILKNNQDMVLRYKSVINDGLQLDLGHA 227
             +    IL+N+   +   K+ +N    +D GHA
Sbjct: 266 TVKWCREILRNSPTAIRVLKAALN---AVDDGHA 296

BLAST of MC07g0841 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 108.6 bits (270), Expect = 7.7e-24
Identity = 73/237 (30.80%), Postives = 123/237 (51.90%), Query Frame = 0

Query: 1   MDPSSPE--KLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIV 60
           ++ S PE  KL  +    +GI  V ++RP + N++ + M+  L +A +S+ +D S RV++
Sbjct: 35  IETSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVM 94

Query: 61  L-SGSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQ---MERCRKPIIGAIAGFAITAGF 120
           + S     FC+G DL     +   +V    +        +E    P I AI G A+  G 
Sbjct: 95  IRSLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGL 154

Query: 121 EIALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEA 180
           E+ALACD+ I  + A F        I P  G +Q+LSR++G + ++E+  T   + A EA
Sbjct: 155 EMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEA 214

Query: 181 ERRGLVNHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEK 232
             +GLVN  V  GE  +KA E+A+ I +     +   K  I++G++ ++   L +E+
Sbjct: 215 ANKGLVNICVTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVEE 271

BLAST of MC07g0841 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 103.6 bits (257), Expect = 2.5e-22
Identity = 65/201 (32.34%), Postives = 103/201 (51.24%), Query Frame = 0

Query: 17  NGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSGRAFCSGVDLSA-- 76
           + +  + INRP  LN+L+    ++   A+ SLD++  V VI+LSG+G+ FCSG+DL++  
Sbjct: 18  SSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGAGKHFCSGIDLNSLS 77

Query: 77  --------------AEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 136
                         + E  +  +K +++  +  +E+CRKP+I AI G  I  G ++  AC
Sbjct: 78  SISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIAAIHGACIGGGVDLITAC 137

Query: 137 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 196
           DI   ++ A F        I    G  Q+L  I+G   A E++LTA   +  EA+  GLV
Sbjct: 138 DIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTARRFSGSEAKDLGLV 197

Query: 197 NHVV-EGGELLKKAREVAEAI 201
           + V     EL      +AE I
Sbjct: 198 SKVFGSKSELDNGVTTIAEGI 217

BLAST of MC07g0841 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 103.2 bits (256), Expect = 3.2e-22
Identity = 60/183 (32.79%), Postives = 97/183 (53.01%), Query Frame = 0

Query: 17  NGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLSGSGRAFCSGVDLSA-- 76
           + +  + INRP  LN+L+    ++   A+ SLD++  V VI+LSG+G+ FCSG+DL++  
Sbjct: 18  SSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGAGKHFCSGIDLNSLS 77

Query: 77  --------------AEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 136
                         + E  +  +K +++  +  +E+CRKP+I AI G  I  G ++  AC
Sbjct: 78  SISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIAAIHGACIGGGVDLITAC 137

Query: 137 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 184
           DI   ++ A F        I    G  Q+L  I+G   A E++LTA   +  EA+  GLV
Sbjct: 138 DIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTARRFSGSEAKDLGLV 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NL242.8e-11177.48Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=E... [more]
P520468.6e-3636.65Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /... [more]
Q1ZXF13.0e-3336.78Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689... [more]
P640179.8e-3233.33Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /... [more]
O071379.8e-3234.54Probable enoyl-CoA hydratase echA8 OS=Mycobacterium leprae (strain TN) OX=272631... [more]
Match NameE-valueIdentityDescription
XP_022138775.18.90e-182100.00probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Momordica charantia][more]
XP_023548460.18.05e-16992.05probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo][more]
XP_022959404.14.65e-16891.29probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata][more]
KAG6575117.12.69e-16791.29putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma sub... [more]
XP_023006283.15.42e-16791.29probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CAG24.31e-182100.00probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX... [more]
A0A6J1H7Z02.25e-16891.29probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1L4H02.63e-16791.29probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1L1R64.35e-16690.91probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=36... [more]
A0A1S3C8X42.93e-16490.53probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497... [more]
Match NameE-valueIdentityDescription
AT4G16210.12.0e-11277.48enoyl-CoA hydratase/isomerase A [more]
AT1G60550.11.1e-2535.98enoyl-CoA hydratase/isomerase D [more]
AT4G16800.17.7e-2430.80ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT5G43280.12.5e-2232.34delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT5G43280.23.2e-2232.79delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.226.10coord: 4..237
e-value: 1.2E-67
score: 229.6
NoneNo IPR availablePANTHERPTHR43802ENOYL-COA HYDRATASEcoord: 6..260
NoneNo IPR availableCDDcd06558crotonase-likecoord: 10..200
e-value: 4.09436E-70
score: 212.421
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 15..240
e-value: 8.8E-53
score: 179.2
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 8..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0841.1MC07g0841.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009536 plastid
molecular_function GO:0003824 catalytic activity