MC01g1266 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g1266
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DNA oxidative demethylase ALKBH2
Location	MC01: 17595584 .. 17596812 (-)
RNA-Seq Expression	MC01g1266
Synteny	MC01g1266

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAAAGCATGAACTATATATAAATTGTAATCATAATCGGCGCCATGAATCAGAACGAAGGTGGGACAATGACTTTGAGGTTGAAGGAAGTGACGGACTCAGAATCGGAGACGAGAATCCCTCGTCCTCTTGATTTGGGAAGACGACACACATTGGATCTCGGAAATGGAAGCGAAATCGTCTACATGACCAAGTTCGTGCCCTCCGATAAGGCTTGGACCTGGTTCGATTTCCTCAATCAACGCATTCCCTGGACCAGGCCTACCATTCGCGTCTTCGGACGCTCCCTACTCCAGGTATTCCTCTTTCTCGATTCTCTTCTCTCTTTTCAGACGTTGTCAAATGTCATACTAATTAGAAATTGAAGATTTAACCAGTGACTGTTGGCTGTGTTGGATTATTTTACTGTTTGCCCGCCCTCTGCTTGAAATTAGGAAGCATTTTCACGCGATTGAGGTTGTCAGATTGAAGTTTTAATTTGCTAATGCAGCCTAGAGACACTTGCTACGTTGCGAGTCCAGGATTGACGACATTAACTTATAGTGGGTACAAACCACGTGCCTATACTTGGGATGATTTTCCTCCACTGAAGGAAATTTTGGACGCTGTAAGACTTTTCCTTTCAATCTTTCGCTATCAGGTTAATTTTGGCTTCTTTAGCTATAACTCCTCGTTACTAGTGGCTCCCCTGATTGTCTGAACTTTTCTCTGTCAAGGTCCACGAAGCTCTTCCTGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAGCTGTATGCACTGAACCAGGAGATTGCTTCGGTATCGTTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGCCCAACAAGACTTCTCAACGTTTTTTGTTTATTTCCATCTGTTTTTGTTGTCTCTGTTCAGATTTTGAAAAGCCCTTTTCTCAAATCTTCAGTCTTTTGCTCAATTTCTTTCTCGCCATTTTAAAATTTATTTGATGTGGCTCCAGGAAGAAATGATGAGGAACCTCCAAGCAAGAAATCAAAGAAGAGCGGTGTGGCTGATACACACTCATTTGCTCTAAAGCACGGTTCACTTTTGGTTATGAAAGGCTACACTCAGCGGGATTGGATGCATTCGGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATTAACCTTACTTTCAGGCATGTTGTTGTTGAT

mRNA sequence

CGAAAGCATGAACTATATATAAATTGTAATCATAATCGGCGCCATGAATCAGAACGAAGGTGGGACAATGACTTTGAGGTTGAAGGAAGTGACGGACTCAGAATCGGAGACGAGAATCCCTCGTCCTCTTGATTTGGGAAGACGACACACATTGGATCTCGGAAATGGAAGCGAAATCGTCTACATGACCAAGTTCGTGCCCTCCGATAAGGCTTGGACCTGGTTCGATTTCCTCAATCAACGCATTCCCTGGACCAGGCCTACCATTCGCGTCTTCGGACGCTCCCTACTCCAGCCTAGAGACACTTGCTACGTTGCGAGTCCAGGATTGACGACATTAACTTATAGTGGGTACAAACCACGTGCCTATACTTGGGATGATTTTCCTCCACTGAAGGAAATTTTGGACGCTGTCCACGAAGCTCTTCCTGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAGCTGTATGCACTGAACCAGGAGATTGCTTCGGTATCGTTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGCCCAACAAGACTTCTCAACGAAGAAATGATGAGGAACCTCCAAGCAAGAAATCAAAGAAGAGCGGTGTGGCTGATACACACTCATTTGCTCTAAAGCACGGTTCACTTTTGGTTATGAAAGGCTACACTCAGCGGGATTGGATGCATTCGGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATTAACCTTACTTTCAGGCATGTTGTTGTTGAT

Coding sequence (CDS)

ATGAATCAGAACGAAGGTGGGACAATGACTTTGAGGTTGAAGGAAGTGACGGACTCAGAATCGGAGACGAGAATCCCTCGTCCTCTTGATTTGGGAAGACGACACACATTGGATCTCGGAAATGGAAGCGAAATCGTCTACATGACCAAGTTCGTGCCCTCCGATAAGGCTTGGACCTGGTTCGATTTCCTCAATCAACGCATTCCCTGGACCAGGCCTACCATTCGCGTCTTCGGACGCTCCCTACTCCAGCCTAGAGACACTTGCTACGTTGCGAGTCCAGGATTGACGACATTAACTTATAGTGGGTACAAACCACGTGCCTATACTTGGGATGATTTTCCTCCACTGAAGGAAATTTTGGACGCTGTCCACGAAGCTCTTCCTGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAGCTGTATGCACTGAACCAGGAGATTGCTTCGGTATCGTTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGCCCAACAAGACTTCTCAACGAAGAAATGATGAGGAACCTCCAAGCAAGAAATCAAAGAAGAGCGGTGTGGCTGATACACACTCATTTGCTCTAAAGCACGGTTCACTTTTGGTTATGAAAGGCTACACTCAGCGGGATTGGATGCATTCGGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATTAACCTTACTTTCAGGCATGTTGTTGTTGAT

Protein sequence

MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVVVD

Homology

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BLAST of MC01g1266 vs. ExPASy Swiss-Prot
Match: Q9SIE0 (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 320.9 bits (821), Expect = 1.3e-86
Identity = 147/219 (67.12%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 34  RHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVAS 93
           R T+DLG+GS+++Y+ +F+P  ++WT+FD+L++ IPWTRPTIRVFGRS LQPRDTCYVAS
Sbjct: 96  RKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPWTRPTIRVFGRSCLQPRDTCYVAS 155

Query: 94  PGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSD 153
            GLT L YSGY+P +Y+WDDFPPLKEILDA+++ LPGS FNSLLLNRYKG +DYV WH+D
Sbjct: 156 SGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGASDYVAWHAD 215

Query: 154 DEKLYALNQEIASVSFGCERDFLLKKKPNKTSQ--RRNDEEPPSKKSKKSGVADTHSFAL 213
           DEK+Y    EIASVSFGCERDF+LKKK ++ S   +  D  P  K+ K+S   D  S  L
Sbjct: 216 DEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQGKTGDSGPAKKRLKRSSREDQQSLTL 275

Query: 214 KHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 251
           KHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTFR V+
Sbjct: 276 KHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTFRLVL 314

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BLAST of MC01g1266 vs. ExPASy Swiss-Prot
Match: Q58DM4 (DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.8e-22
Identity = 67/176 (38.07%), Postives = 92/176 (52.27%), Query Frame = 0


Query: 75  IRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFN 134
           ++VFG+    PR        GL T T+SG       W   P L+ + D V   + G +FN
Sbjct: 98  VQVFGKWHSVPRKQATYGDTGL-TYTFSGLTLSPKPW--IPVLERVRDRV-SLVTGQTFN 157

Query: 135 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPP 194
            +L+NRYK G D++G H DDE+  AL   IASVSFG  RDF+ + K ++          P
Sbjct: 158 FVLINRYKDGQDHIGEHRDDERELALGSPIASVSFGACRDFVFRHKDSRGKH-------P 217

Query: 195 SKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 251
           S++       +     L HGSLL+M   T   W HS+P R K  A R+NLTFR ++
Sbjct: 218 SRR------LEVVRLQLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKIL 256

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BLAST of MC01g1266 vs. ExPASy Swiss-Prot
Match: Q6P6J4 (DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.0e-21
Identity = 65/176 (36.93%), Postives = 90/176 (51.14%), Query Frame = 0


Query: 75  IRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFN 134
           ++VFG+    PR        GL T T+SG       W   P L+ + D V E + G +FN
Sbjct: 77  VQVFGKWHSVPRKQATYGDAGL-TYTFSGLTLTPKPW--VPVLERVRDRVCE-VTGQTFN 136

Query: 135 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPP 194
            +L+NRYK G D++G H DDE+  A    IASVSFG  RDF+ + K ++  + R      
Sbjct: 137 FVLVNRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFIFRHKDSRGKRPRR----- 196

Query: 195 SKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 251
                     +     L HGSLL+M   T   W HS+P R +  A R+NLTFR ++
Sbjct: 197 --------TVEVVRLQLAHGSLLMMNPPTNTHWYHSLPIRKRVLAPRVNLTFRKIL 235

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BLAST of MC01g1266 vs. ExPASy Swiss-Prot
Match: Q6NS38 (DNA oxidative demethylase ALKBH2 OS=Homo sapiens OX=9606 GN=ALKBH2 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.3e-21
Identity = 69/177 (38.98%), Postives = 91/177 (51.41%), Query Frame = 0


Query: 75  IRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFN 134
           ++VFG+    PR        GL T T+SG       W   P L+ I D V   + G +FN
Sbjct: 99  VQVFGKWHSVPRKQATYGDAGL-TYTFSGLTLSPKPW--IPVLERIRDHV-SGVTGQTFN 158

Query: 135 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPP 194
            +L+NRYK G D++G H DDE+  A    IASVSFG  RDF+ + K ++           
Sbjct: 159 FVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSR----------- 218

Query: 195 SKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVVV 252
             KS    VA      L HGSLL+M   T   W HS+P R K  A R+NLTFR +++
Sbjct: 219 -GKSPSRRVAVVR-LPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILL 258

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BLAST of MC01g1266 vs. ExPASy Swiss-Prot
Match: Q32L00 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 GN=ALKBH3 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 4.4e-21
Identity = 80/239 (33.47%), Postives = 108/239 (45.19%), Query Frame = 0


Query: 20  ESETRIPRPLDLGRRHTLDLGNG----SEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTI 79
           E   R P P  + +    ++       S +     FV   +A + F+ L + +PW + T 
Sbjct: 61  EGVRRAPEPRVIEKEGVYEISMSPTGISRVCLCPGFVDLKEADSVFEQLCRDVPWKQRTG 120

Query: 80  RVFGRSLLQPRDTCYVASPGLTTLTYS--GYKPRAYTWDDFPPLKEILDAVHEALPGSSF 139
                +  QPR T +    G    TYS    +P  +    + P+  +L    E   G SF
Sbjct: 121 IRDDVTYQQPRLTAWY---GELPYTYSRITMEPNPH----WHPVLLMLKNQIEENTGHSF 180

Query: 140 NSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEP 199
           NSLL N Y+   D V WHSDDE        IAS+SFG  R F ++KKP           P
Sbjct: 181 NSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRMFEMRKKP-----------P 240

Query: 200 PSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVVVD 253
           P      + V +     L HG+LL+M+G TQ DW H VPK   +   RINLTFR V  D
Sbjct: 241 PEDNGDYTYV-ERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRINLTFRTVYPD 280

[Close]

BLAST of MC01g1266 vs. NCBI nr
Match: XP_022133026.1 (DNA oxidative demethylase ALKBH2 [Momordica charantia])

HSP 1 Score: 523 bits (1348), Expect = 1.14e-187
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0


Query: 1   MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW 60
           MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW
Sbjct: 1   MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW 60

Query: 61  FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI 120
           FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI
Sbjct: 61  FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI 120

Query: 121 LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 180
           LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK
Sbjct: 121 LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 180

Query: 181 PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 240
           PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT
Sbjct: 181 PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 240

Query: 241 RINLTFRHVVVD 252
           RINLTFRHVVVD
Sbjct: 241 RINLTFRHVVVD 252

[Close]

BLAST of MC01g1266 vs. NCBI nr
Match: XP_038882915.1 (DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882917.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882918.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882919.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882920.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882921.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida])

HSP 1 Score: 476 bits (1225), Expect = 4.83e-169
Identity = 224/244 (91.80%), Postives = 237/244 (97.13%), Query Frame = 0


Query: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68
           M+LRLKEVTDSESET+IPRP + GRR TLDLGNGSEIVY+ KFVPSD+AWTWFDFLNQRI
Sbjct: 1   MSLRLKEVTDSESETKIPRPFNSGRRQTLDLGNGSEIVYVPKFVPSDQAWTWFDFLNQRI 60

Query: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128
           PWTRPTIRVFGRS+LQPRDTCYVA+PGLTTLTYSGYKP AYTWDDFPPLK+ILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFPPLKDILDAVHEAL 120

Query: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRR 188
           PGSSFNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 189 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 248
           NDEEPP+KKSKKS VAD+HSF LKHGSLLVM+GYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADSHSFVLKHGSLLVMRGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 249 VVVD 252
           V+VD
Sbjct: 241 VIVD 244

[Close]

BLAST of MC01g1266 vs. NCBI nr
Match: XP_004134811.1 (DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742940.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >KGN49032.1 hypothetical protein Csa_003044 [Cucumis sativus])

HSP 1 Score: 466 bits (1200), Expect = 3.12e-165
Identity = 220/244 (90.16%), Postives = 233/244 (95.49%), Query Frame = 0


Query: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68
           M+LR KEVTDSESET+IPRP DLG+R TLDLGNGSEI+Y++KFV SD+AWTWFDFLNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128
           PWTRPTI VFGRS+LQPRDTCYVA+PGLTTLTYSGYKP AYTWDDF PLK+ILDAVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRR 188
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 189 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 248
           NDEEPP+KKSKKS VAD HSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 249 VVVD 252
           V+VD
Sbjct: 241 VIVD 244

[Close]

BLAST of MC01g1266 vs. NCBI nr
Match: XP_022950477.1 (DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 DNA oxidative demethylase ALKBH2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 465 bits (1196), Expect = 5.84e-164
Identity = 221/249 (88.76%), Postives = 234/249 (93.98%), Query Frame = 0


Query: 1   MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW 60
           +N  E G M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTW
Sbjct: 7   VNYEEFGRMSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTW 66

Query: 61  FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI 120
           FDFLNQ IPWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+I
Sbjct: 67  FDFLNQHIPWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDI 126

Query: 121 LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 180
           L+AVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK
Sbjct: 127 LNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 186

Query: 181 PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 240
            NKTSQRRNDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEAT
Sbjct: 187 SNKTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 246

Query: 241 RINLTFRHV 249
           RINLTFRHV
Sbjct: 247 RINLTFRHV 255

[Close]

BLAST of MC01g1266 vs. NCBI nr
Match: KAG6603836.1 (DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461 bits (1186), Expect = 3.95e-163
Identity = 218/241 (90.46%), Postives = 230/241 (95.44%), Query Frame = 0


Query: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68
           M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTWFDFLNQ I
Sbjct: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60

Query: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128
           PWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120

Query: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRR 188
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180

Query: 189 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 248
           NDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

[Close]

BLAST of MC01g1266 vs. ExPASy TrEMBL
Match: A0A6J1BTV9 (DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 PE=4 SV=1)

HSP 1 Score: 523 bits (1348), Expect = 5.52e-188
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0


Query: 1   MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW 60
           MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW
Sbjct: 1   MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW 60

Query: 61  FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI 120
           FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI
Sbjct: 61  FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI 120

Query: 121 LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 180
           LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK
Sbjct: 121 LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 180

Query: 181 PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 240
           PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT
Sbjct: 181 PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 240

Query: 241 RINLTFRHVVVD 252
           RINLTFRHVVVD
Sbjct: 241 RINLTFRHVVVD 252

[Close]

BLAST of MC01g1266 vs. ExPASy TrEMBL
Match: A0A0A0KL20 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510990 PE=4 SV=1)

HSP 1 Score: 466 bits (1200), Expect = 1.51e-165
Identity = 220/244 (90.16%), Postives = 233/244 (95.49%), Query Frame = 0


Query: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68
           M+LR KEVTDSESET+IPRP DLG+R TLDLGNGSEI+Y++KFV SD+AWTWFDFLNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128
           PWTRPTI VFGRS+LQPRDTCYVA+PGLTTLTYSGYKP AYTWDDF PLK+ILDAVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRR 188
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 189 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 248
           NDEEPP+KKSKKS VAD HSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 249 VVVD 252
           V+VD
Sbjct: 241 VIVD 244

[Close]

BLAST of MC01g1266 vs. ExPASy TrEMBL
Match: A0A6J1GEX9 (DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 2.83e-164
Identity = 221/249 (88.76%), Postives = 234/249 (93.98%), Query Frame = 0


Query: 1   MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW 60
           +N  E G M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTW
Sbjct: 7   VNYEEFGRMSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTW 66

Query: 61  FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI 120
           FDFLNQ IPWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+I
Sbjct: 67  FDFLNQHIPWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDI 126

Query: 121 LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 180
           L+AVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK
Sbjct: 127 LNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 186

Query: 181 PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 240
            NKTSQRRNDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEAT
Sbjct: 187 SNKTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 246

Query: 241 RINLTFRHV 249
           RINLTFRHV
Sbjct: 247 RINLTFRHV 255

[Close]

BLAST of MC01g1266 vs. ExPASy TrEMBL
Match: A0A6J1GEX8 (DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 460 bits (1184), Expect = 3.51e-162
Identity = 219/247 (88.66%), Postives = 232/247 (93.93%), Query Frame = 0


Query: 1   MNQNEGGTMTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTW 60
           +N  E G M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTW
Sbjct: 7   VNYEEFGRMSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTW 66

Query: 61  FDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEI 120
           FDFLNQ IPWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+I
Sbjct: 67  FDFLNQHIPWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDI 126

Query: 121 LDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 180
           L+AVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK
Sbjct: 127 LNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK 186

Query: 181 PNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 240
            NKTSQRRNDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEAT
Sbjct: 187 SNKTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEAT 246

Query: 241 RINLTFR 247
           RINLTFR
Sbjct: 247 RINLTFR 253

[Close]

BLAST of MC01g1266 vs. ExPASy TrEMBL
Match: A0A5A7SMW2 (DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G00710 PE=4 SV=1)

HSP 1 Score: 457 bits (1175), Expect = 9.78e-162
Identity = 214/244 (87.70%), Postives = 229/244 (93.85%), Query Frame = 0


Query: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68
           M+LR KE TDSE+ET+IPRP DLG+R TLDLGNGSEI+Y++KFV SD+AWTWFD+LNQRI
Sbjct: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128
           PWTRPTIRVFGRS+LQPRDTCYVA PGLTTLTYSGYKP AYTWDDF PLK+ILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRR 188
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 189 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 248
           NDE PP+KKSKK   ADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEVPPAKKSKKCNGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 249 VVVD 252
           ++ D
Sbjct: 241 IIGD 244

[Close]

BLAST of MC01g1266 vs. TAIR 10
Match: AT2G22260.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 320.9 bits (821), Expect = 9.4e-88
Identity = 147/219 (67.12%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 34  RHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVAS 93
           R T+DLG+GS+++Y+ +F+P  ++WT+FD+L++ IPWTRPTIRVFGRS LQPRDTCYVAS
Sbjct: 96  RKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPWTRPTIRVFGRSCLQPRDTCYVAS 155

Query: 94  PGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSD 153
            GLT L YSGY+P +Y+WDDFPPLKEILDA+++ LPGS FNSLLLNRYKG +DYV WH+D
Sbjct: 156 SGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGASDYVAWHAD 215

Query: 154 DEKLYALNQEIASVSFGCERDFLLKKKPNKTSQ--RRNDEEPPSKKSKKSGVADTHSFAL 213
           DEK+Y    EIASVSFGCERDF+LKKK ++ S   +  D  P  K+ K+S   D  S  L
Sbjct: 216 DEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQGKTGDSGPAKKRLKRSSREDQQSLTL 275

Query: 214 KHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 251
           KHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTFR V+
Sbjct: 276 KHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTFRLVL 314

The following BLAST results are available for this feature:

BLAST of MC01g1266 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIE0	1.3e-86	67.12	DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 ...	[more]
Q58DM4	1.8e-22	38.07	DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1	[more]
Q6P6J4	2.0e-21	36.93	DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1	[more]
Q6NS38	3.3e-21	38.98	DNA oxidative demethylase ALKBH2 OS=Homo sapiens OX=9606 GN=ALKBH2 PE=1 SV=1	[more]
Q32L00	4.4e-21	33.47	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 G...	[more]

BLAST of MC01g1266 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133026.1	1.14e-187	100.00	DNA oxidative demethylase ALKBH2 [Momordica charantia]	[more]
XP_038882915.1	4.83e-169	91.80	DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidati...	[more]
XP_004134811.1	3.12e-165	90.16	DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative...	[more]
XP_022950477.1	5.84e-164	88.76	DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 D...	[more]
KAG6603836.1	3.95e-163	90.46	DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of MC01g1266 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTV9	5.52e-188	100.00	DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 ...	[more]
A0A0A0KL20	1.51e-165	90.16	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1GEX9	2.83e-164	88.76	DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1GEX8	3.51e-162	88.66	DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5A7SMW2	9.78e-162	87.70	DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of MC01g1266 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22260.1	9.4e-88	67.12	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 45..247 e-value: 1.8E-31 score: 109.8
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 33..251 e-value: 5.7E-60 score: 204.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..204
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..203
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 40..249
IPR032852	DNA oxidative demethylase ALKBH2	PANTHER	PTHR31573	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2	coord: 30..250
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 132..250 score: 12.816041

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g1266.1	MC01g1266.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006307	DNA dealkylation involved in DNA repair
biological_process	GO:0032259	methylation
biological_process	GO:0035511	oxidative DNA demethylation
biological_process	GO:0006281	DNA repair
molecular_function	GO:0051747	cytosine C-5 DNA demethylase activity
molecular_function	GO:0043734	DNA-N1-methyladenine dioxygenase activity
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0008168	methyltransferase activity