Csor.00g296810 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g296810 vs. ExPASy Swiss-Prot
Match: F4HYR6 (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana OX=3702 GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 7.3e-197
Identity = 357/595 (60.00%), Postives = 427/595 (71.76%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +N  AV TT S+A KL +  GVK VE D+GV+ MTTYTP FL +P +             
Sbjct: 105 INAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYTPDFLELPQQVWQKISNEGDRRA 164

Query: 61  GEGIVIGFVDSGIDPTHPSFG-------FDDGYEDLRLF--CEKGRFFPLSSCNGKIVGA 120
           GE IVIGFVD+GI+PTHPSF        +      L     CE G FFP  SCNGKI+ A
Sbjct: 165 GEDIVIGFVDTGINPTHPSFAALDLTNPYSSNLSRLHFSGDCEIGPFFPPGSCNGKIISA 224

Query: 121 RFFSEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPR 180
           RFFS GARA   LNSS+D LSPFDA GHGSHVASIAAGN GVPV V+GFFYG ASGMAPR
Sbjct: 225 RFFSAGARASGALNSSLDILSPFDASGHGSHVASIAAGNAGVPVIVDGFFYGRASGMAPR 284

Query: 181 ARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILS 240
           +RIAVYKA+YP++ TL DV++AIDQA++DGVD+L LSVGP+EPP +  T L I+D+A+L 
Sbjct: 285 SRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSVGPDEPPVDKPTVLGIFDLAMLL 344

Query: 241 ATRAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSG 300
           A +AG+ VVQAVGNNGP+P++V+SYSPW VGVAA  TDR YPA L+L  GQ V GVGLSG
Sbjct: 345 ARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTDRSYPAPLILDGGQTVQGVGLSG 404

Query: 301 PT----FFLHKLVLAKDAMNQNG------TMPSEECQYPEAFDPNLVQSSIVLCSFSQGF 360
           PT       H+LVLAKDA+  NG      T   EECQ PE FDP  V  SIV+C+FS GF
Sbjct: 405 PTLGAPLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSIVICTFSDGF 464

Query: 361 LNGTSSLAAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYY 420
            N  S++ AI  TA TL F+GF LIANP +GD++AEP+ F  PGILIP  S +Q IL+YY
Sbjct: 465 YNQMSTVLAITQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVSAAQIILRYY 524

Query: 421 EANTCKDDNGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPD 480
           E  T +D  G+ ++F  +A IGEGR + F  +AP VSRFSSRGP +++  R+  DVLKPD
Sbjct: 525 EEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPD 584

Query: 481 ILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIA 540
           ILAPGHQIW AWS  S  +P+L G  FA++SGTSMA PHI GI ALIKQ NPSWTP+MIA
Sbjct: 585 ILAPGHQIWGAWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIA 644

Query: 541 SAMSTTATKYDMNGDLIQAEGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
           SA+STTA +YD NG++I AE + + RL+PS  FD GAG V P  ALDPGLV PAG
Sbjct: 645 SAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAG 699

BLAST of Csor.00g296810 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 7.9e-143
Identity = 285/590 (48.31%), Postives = 375/590 (63.56%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGFA H +P +A  LR+A GV+ V++D  VR++TT+TP+FLG+P+   V          
Sbjct: 93  INGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTD--VWPTGGGFDRA 152

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDD--GYEDLRLF---CEKGRFFPLSSCNGKIVGARFFS 120
           GE IVIGFVDSGI P HPSF       Y  L  +   CE+      S CN KIVGA+ F+
Sbjct: 153 GEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFCNRKIVGAQHFA 212

Query: 121 EGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIA 180
           E A+A    N  +D+ SP D +GHGSH A+IAAGN G+P+ ++G+ +G ASGMAPRARIA
Sbjct: 213 EAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHGYEFGKASGMAPRARIA 272

Query: 181 VYKAVYPTM-STLTDVVSAIDQAVIDGVDILALSVGPNEPP-EEGLTFLSIYDIAILSAT 240
           VYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TFL+ +D  +L A 
Sbjct: 273 VYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTTKTTFLNPFDATLLGAV 332

Query: 241 RAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT 300
           +AG+ V QA GN GP P T+VSYSPW   VAA+  DR Y   L LGNG+ + G+GLS PT
Sbjct: 333 KAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNGKMLAGMGLSPPT 392

Query: 301 --FFLHKLVLAKDAMNQNGTMP--SEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLA 360
               L+ LV A D +  +        +CQ PE F+  LV+ +I+LC +S  F+ GT+S+ 
Sbjct: 393 RPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNKKLVEGNILLCGYSFNFVVGTASIK 452

Query: 361 AIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDD 420
            ++ TA  L   GF L+          +P+P  +PGILI + S S  ++ YY A+T +D 
Sbjct: 453 KVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDVSKSMDLIDYYNASTSRDW 512

Query: 421 NGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQI 480
            G V  FK + +IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ I
Sbjct: 513 TGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLI 572

Query: 481 WAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTAT 540
           WAAW P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T
Sbjct: 573 WAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTST 632

Query: 541 KYDMNGDLIQAEGFN---IHRLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
             D  G L+QA+ ++      L  +TPFD G+G V P+ ALDPGL+F AG
Sbjct: 633 VIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAG 680

BLAST of Csor.00g296810 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.4e-139
Identity = 280/591 (47.38%), Postives = 370/591 (62.61%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGFA H +P +A  LR+A GVK V+RD  VRK+TT+TP+FLG+P+   V          
Sbjct: 93  INGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTD--VWPTGGGYDRA 152

Query: 61  GEGIVIGFVDSGIDPTHPSFGFD------DGYEDLRLFCEKGRFFPLSSCNGKIVGARFF 120
           GE IVIGF+DSGI P HPSF           +   +  CE+     +S CNGKI+GA+ F
Sbjct: 153 GEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISFCNGKIIGAQHF 212

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
           +E A+A    N  +DF SP D +GHGSH A+IAAGN G+PV ++G+ +G ASGMAPRARI
Sbjct: 213 AEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNNGIPVRMHGYEFGKASGMAPRARI 272

Query: 181 AVYKAVYPTM-STLTDVVSAIDQAVIDGVDILALSVGPNEPP-EEGLTFLSIYDIAILSA 240
           AVYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TFL+ +D  +L A
Sbjct: 273 AVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATTKTTFLNPFDATLLGA 332

Query: 241 TRAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGP 300
            +AG+ V QA GN GP P T+VSYSPW   VAA+  DR Y   L LGNG+ + G+GLS  
Sbjct: 333 VKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNGKMLAGIGLSPS 392

Query: 301 T--FFLHKLVLAKDAMNQNGTMP--SEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSL 360
           T     +K+V A D +  +  M     +CQ PE  +  LV+ +I+LC +S  F+ G++S+
Sbjct: 393 TRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLNKKLVEGNILLCGYSFNFVAGSASI 452

Query: 361 AAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKD 420
             +  TA  L   GF L+          +P+P  +PGILI + S S  ++ YY   T +D
Sbjct: 453 KKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIPGILITDVSKSMDLIDYYNVTTSRD 512

Query: 421 DNGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQ 480
             G V +FK + +IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  
Sbjct: 513 WMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSL 572

Query: 481 IWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTA 540
           IW+AWS     E    G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+
Sbjct: 573 IWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTS 632

Query: 541 TKYDMNGDLIQAEGFN---IHRLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
           T  D  G  +QA+ ++      L  +TPFD G+G V P+ ALDPGL+F AG
Sbjct: 633 TVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAG 681

BLAST of Csor.00g296810 vs. ExPASy Swiss-Prot
Match: Q9SA75 (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.5e-130
Identity = 268/592 (45.27%), Postives = 366/592 (61.82%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGF+   T  +A +L     V+ V  D  V K TT+TP+FLG+P  + +    ++ +  
Sbjct: 105 INGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYA-- 164

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRL------FCEKGRFFPLSSCNGKIVGARFF 120
           GEG+VIGF+D+GIDPTHPSF          +       CE    FP  SCN K++GAR F
Sbjct: 165 GEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHF 224

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
           +E A +   LNSS D  SPFD EGHG+H AS+AAGN G+PV V G   G ASGMAPRA I
Sbjct: 225 AESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHI 284

Query: 181 AVYKAVYPTMSTL-TDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSAT 240
           A+YKA+Y        D+++AIDQA  DGVDI+ LS+ PN  P    TF +  D+A+LSA 
Sbjct: 285 AIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAV 344

Query: 241 RAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT 300
           +AGI VVQA GN GPAP ++ S+SPW   V A+  DRVY  S++LGN   + GVGL+  T
Sbjct: 345 KAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT 404

Query: 301 FFLHKLVLAKDAMNQNGTMPSE-----ECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSL 360
             +HKLVLA  A+ +NGT   +     ECQ   +FD  LVQ  I++CS++  F+ G S++
Sbjct: 405 RIMHKLVLATHAL-RNGTTVMDAIYVGECQDSSSFDQKLVQGKILVCSYTVRFILGVSTI 464

Query: 361 AAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKD 420
              + TA  L   G     +P+   F     P  +PGILI +  DSQA+L+YY ++  ++
Sbjct: 465 KQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILISSPQDSQALLRYYNSSLLRE 524

Query: 421 D-NGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGH 480
           + +G +      A I  G   ++G  AP V  FS+RGPD  + +   AD++KP+++APG+
Sbjct: 525 NGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDPEDDSFVDADIMKPNLVAPGN 584

Query: 481 QIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTT 540
            IW AWSPL +     +G +FA+ SGTSM+APH+ GIAALIKQK P +TP+ IASA+STT
Sbjct: 585 AIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAALIKQKFPHFTPAAIASALSTT 644

Query: 541 ATKYDMNGDLIQAEGFNIH---RLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
           A+  D  G+ I A+   ++      P+TPFD+G+G V  T ALDPGL+F  G
Sbjct: 645 ASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNATAALDPGLIFDIG 693

BLAST of Csor.00g296810 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.6e-130
Identity = 265/590 (44.92%), Postives = 359/590 (60.85%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGFA+     +A KL     V  +  D  VR  TTYTP+F+G+P  + V  K+      
Sbjct: 113 INGFALFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWV--KEGGFEIA 172

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRL------FCEKGRFFPLSSCNGKIVGARFF 120
           GEG++IGF+D+GIDP HPSF  +D      +       CE    FP  SCN K++GAR F
Sbjct: 173 GEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGARHF 232

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
           ++ A      NSS D+ SPFD +GHG+H AS+AAGN GVPV V+   +G+ASG+APRA I
Sbjct: 233 AQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPRAFI 292

Query: 181 AVYKAVYPTMSTL-TDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSAT 240
           +VYKA+Y +      DVV+AIDQA  DGVDIL+LS+ PN  P    TF +  D+A+LSA 
Sbjct: 293 SVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPPGVATFFNPIDMALLSAV 352

Query: 241 RAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT 300
           +AGI VVQA GN GPAP T+ S+SPW   V AS  DRVY  SL LGN   + G+G + PT
Sbjct: 353 KAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFAIPT 412

Query: 301 FF--LHKLVLAKDAMNQNGTMPSE----ECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSS 360
               ++K++ A  A+N + ++  +    ECQ  E FD + V   +++CS+S  F+ G S+
Sbjct: 413 DSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLST 472

Query: 361 LAAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCK 420
           +   +  A  L   G     +P    F   P P  +PGI+IP+  DS+ +LKYY ++  +
Sbjct: 473 IKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQR 532

Query: 421 D-DNGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPG 480
           D     +  F   AAI  G  A+F ++AP V  +S+RGPD  + +   ADVLKP+++APG
Sbjct: 533 DVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPG 592

Query: 481 HQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMST 540
           + IW AWS  S      +G +FA+MSGTSMAAPH+ G+AALIKQ  P +TPS I+SA+ST
Sbjct: 593 NSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALST 652

Query: 541 TATKYDMNGDLIQAEGFNIH---RLYPSTPFDLGAGLVKPTNALDPGLVF 574
           TA   D  G  I A+    +    LY +TP D+G+G V  T ALDPGLVF
Sbjct: 653 TALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVF 700

BLAST of Csor.00g296810 vs. NCBI nr
Match: KAG6573471.1 (Subtilisin-like protease 2.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1154 bits (2986), Expect = 0.0
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD
Sbjct: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120
           GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA
Sbjct: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120

Query: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180
           VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV
Sbjct: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180

Query: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240
           YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV
Sbjct: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240

Query: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300
           QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV
Sbjct: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300

Query: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360
           LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF
Sbjct: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360

Query: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420
           IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA
Sbjct: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420

Query: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480
           AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE
Sbjct: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480

Query: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540
           PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA
Sbjct: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540

Query: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAGKFQ 579
           EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAGKFQ
Sbjct: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAGKFQ 579

BLAST of Csor.00g296810 vs. NCBI nr
Match: XP_022954879.1 (subtilisin-like protease SBT2.4 [Cucurbita moschata])

HSP 1 Score: 1144 bits (2960), Expect = 0.0
Identity = 574/575 (99.83%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD
Sbjct: 83  MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 142

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120
           GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA
Sbjct: 143 GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 202

Query: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180
           VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV
Sbjct: 203 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 262

Query: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240
           YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV
Sbjct: 263 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 322

Query: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300
           QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV
Sbjct: 323 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 382

Query: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360
           LAKDAMNQNGTMPSEECQYPEAFDPNLVQ+SIVLCSFSQGFLNGTSSLAAIIHTATTLRF
Sbjct: 383 LAKDAMNQNGTMPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 442

Query: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420
           IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA
Sbjct: 443 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 502

Query: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480
           AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE
Sbjct: 503 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 562

Query: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540
           PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA
Sbjct: 563 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 622

Query: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA
Sbjct: 623 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 657

BLAST of Csor.00g296810 vs. NCBI nr
Match: XP_023523808.1 (subtilisin-like protease SBT2.4 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 565/575 (98.26%), Postives = 570/575 (99.13%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD
Sbjct: 86  MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 145

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120
           GEGIV+GFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFS GARA
Sbjct: 146 GEGIVVGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSAGARA 205

Query: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180
           VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV
Sbjct: 206 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 265

Query: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240
           YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV
Sbjct: 266 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 325

Query: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300
           QAVGNNGPAPATVVSYSPW+VGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV
Sbjct: 326 QAVGNNGPAPATVVSYSPWAVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 385

Query: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360
           LAKDAM QNGT+PSEECQYPEAFDPNLVQ+SIVLCSFSQGFLNGTSSL AIIHTATTLRF
Sbjct: 386 LAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLTAIIHTATTLRF 445

Query: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420
           IGFALIANPNYGDFIAEPIPF LPGILIPNASDSQAILKYYEANTCKD NGMVSEFKGKA
Sbjct: 446 IGFALIANPNYGDFIAEPIPFRLPGILIPNASDSQAILKYYEANTCKDANGMVSEFKGKA 505

Query: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480
           AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE
Sbjct: 506 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 565

Query: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540
           PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA
Sbjct: 566 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 625

Query: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           EGF+IHRLYPSTPFDLGAGLVKPTNALDPGLVFPA
Sbjct: 626 EGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 660

BLAST of Csor.00g296810 vs. NCBI nr
Match: XP_022994742.1 (subtilisin-like protease SBT2.4 [Cucurbita maxima])

HSP 1 Score: 1116 bits (2886), Expect = 0.0
Identity = 561/575 (97.57%), Postives = 566/575 (98.43%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSR+CSKKTKTSPD
Sbjct: 86  MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRLCSKKTKTSPD 145

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120
           GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFS GARA
Sbjct: 146 GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSAGARA 205

Query: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180
           VAKLNSSVDFLSPFD EGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV
Sbjct: 206 VAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 265

Query: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240
           YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV
Sbjct: 266 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 325

Query: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300
           QAVGNNGPA ATVVSYSPW+VGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV
Sbjct: 326 QAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 385

Query: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360
           LAKDAM QNGT+PSEECQYPEAFDPNLVQ+SIVLCSFSQGFLNGTSSLAAIIHTA TLRF
Sbjct: 386 LAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRF 445

Query: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420
           IGFALIANPNYGDFIAEPI F LPGILIPNASDSQAIL YYEANTCKD NGMVSEFKGKA
Sbjct: 446 IGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKA 505

Query: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480
           AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE
Sbjct: 506 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 565

Query: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540
           PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA
Sbjct: 566 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 625

Query: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           EGF+IHRLYPSTPFDLGAGLVKPTNALDPGLVFPA
Sbjct: 626 EGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 660

BLAST of Csor.00g296810 vs. NCBI nr
Match: KAG7012613.1 (Subtilisin-like protease SBT2.4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 512/575 (89.04%), Postives = 513/575 (89.22%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD
Sbjct: 67  MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 126

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120
           GEGIVIGFVDSGIDPTHPSFGFDDGYEDL LFCEKGRFFPLSSCNGKIVGA         
Sbjct: 127 GEGIVIGFVDSGIDPTHPSFGFDDGYEDLSLFCEKGRFFPLSSCNGKIVGA--------- 186

Query: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180
                                                               RIAVYKAV
Sbjct: 187 ----------------------------------------------------RIAVYKAV 246

Query: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240
           YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV
Sbjct: 247 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 306

Query: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300
           QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV
Sbjct: 307 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 366

Query: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360
           LAKDAMNQNGTMPSEECQYPEAFDPNLVQ+SIVLCSFSQGFLNGTSSLAAIIHTATTLRF
Sbjct: 367 LAKDAMNQNGTMPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 426

Query: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420
           IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA
Sbjct: 427 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 486

Query: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480
           AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE
Sbjct: 487 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 546

Query: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540
           PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA
Sbjct: 547 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 580

Query: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA
Sbjct: 607 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 580

BLAST of Csor.00g296810 vs. ExPASy TrEMBL
Match: A0A6J1GUB5 (subtilisin-like protease SBT2.4 OS=Cucurbita moschata OX=3662 GN=LOC111457007 PE=3 SV=1)

HSP 1 Score: 1144 bits (2960), Expect = 0.0
Identity = 574/575 (99.83%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD
Sbjct: 83  MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 142

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120
           GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA
Sbjct: 143 GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 202

Query: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180
           VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV
Sbjct: 203 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 262

Query: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240
           YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV
Sbjct: 263 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 322

Query: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300
           QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV
Sbjct: 323 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 382

Query: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360
           LAKDAMNQNGTMPSEECQYPEAFDPNLVQ+SIVLCSFSQGFLNGTSSLAAIIHTATTLRF
Sbjct: 383 LAKDAMNQNGTMPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 442

Query: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420
           IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA
Sbjct: 443 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 502

Query: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480
           AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE
Sbjct: 503 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 562

Query: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540
           PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA
Sbjct: 563 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 622

Query: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA
Sbjct: 623 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 657

BLAST of Csor.00g296810 vs. ExPASy TrEMBL
Match: A0A6J1JWQ4 (subtilisin-like protease SBT2.4 OS=Cucurbita maxima OX=3661 GN=LOC111490380 PE=3 SV=1)

HSP 1 Score: 1116 bits (2886), Expect = 0.0
Identity = 561/575 (97.57%), Postives = 566/575 (98.43%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSR+CSKKTKTSPD
Sbjct: 86  MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRLCSKKTKTSPD 145

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSEGARA 120
           GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFS GARA
Sbjct: 146 GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRLFCEKGRFFPLSSCNGKIVGARFFSAGARA 205

Query: 121 VAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 180
           VAKLNSSVDFLSPFD EGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV
Sbjct: 206 VAKLNSSVDFLSPFDVEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAV 265

Query: 181 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 240
           YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV
Sbjct: 266 YPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVV 325

Query: 241 QAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 300
           QAVGNNGPA ATVVSYSPW+VGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV
Sbjct: 326 QAVGNNGPASATVVSYSPWAVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPTFFLHKLV 385

Query: 301 LAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAAIIHTATTLRF 360
           LAKDAM QNGT+PSEECQYPEAFDPNLVQ+SIVLCSFSQGFLNGTSSLAAIIHTA TLRF
Sbjct: 386 LAKDAMKQNGTIPSEECQYPEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRF 445

Query: 361 IGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKA 420
           IGFALIANPNYGDFIAEPI F LPGILIPNASDSQAIL YYEANTCKD NGMVSEFKGKA
Sbjct: 446 IGFALIANPNYGDFIAEPITFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKA 505

Query: 421 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 480
           AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE
Sbjct: 506 AIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTE 565

Query: 481 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 540
           PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA
Sbjct: 566 PLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQA 625

Query: 541 EGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           EGF+IHRLYPSTPFDLGAGLVKPTNALDPGLVFPA
Sbjct: 626 EGFDIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 660

BLAST of Csor.00g296810 vs. ExPASy TrEMBL
Match: A0A6J1CM39 (subtilisin-like protease SBT2.4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012715 PE=3 SV=1)

HSP 1 Score: 945 bits (2443), Expect = 0.0
Identity = 476/585 (81.37%), Postives = 516/585 (88.21%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVC-SKKTKTSP 60
           +NGFAVHTTPSEA KLR+A GV  VERDRGVRKMTTYTP+FLGVPS  R     K   S 
Sbjct: 100 INGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPSTLRATVGCKRNESS 159

Query: 61  DGEGIVIGFVDSGIDPTHPSFGFDDGY--EDLRLFC---EKGRFFPLSSCNGKIVGARFF 120
            GEGIVIGFVDSGIDPTHPSFGF  G   ++   FC   E+G FF  +SCNGKIVGARFF
Sbjct: 160 GGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSKFCGVCEEGPFFGSNSCNGKIVGARFF 219

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
             GA+AV++LNSSVDFLSPFDAEGHGSHVASIAAGN GVPV VNGFFYG ASG APRARI
Sbjct: 220 CAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFFYGLASGXAPRARI 279

Query: 181 AVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATR 240
           A+YKAVYPT++TLTDVV+AIDQA+IDGVDILALSVGPNEPPE G TFLSIYDIA+LSATR
Sbjct: 280 AIYKAVYPTVATLTDVVAAIDQAIIDGVDILALSVGPNEPPEVGFTFLSIYDIAVLSATR 339

Query: 241 AGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT- 300
           AGILVVQA GNNGPAP+TVVSYSPW+VGVAASGTDRVYPASLLLGNGQ VGG GLSGPT 
Sbjct: 340 AGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLGNGQXVGGTGLSGPTL 399

Query: 301 ---FFLHKLVLAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAA 360
              F LHKLVLAKDA+NQN  +P+EECQYP+AFDPN+VQ+SIV+CSFSQGFLNGTS+L A
Sbjct: 400 GSEFLLHKLVLAKDAVNQNVALPTEECQYPQAFDPNIVQNSIVICSFSQGFLNGTSTLTA 459

Query: 361 IIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDN 420
           I+HTA TLRF+ F L+ANP+YGDFIAEPIPF +PGIL+P+ SDSQ ILKYYE N CKD  
Sbjct: 460 ILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVSDSQVILKYYEENICKDGR 519

Query: 421 GMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 480
           GMV+ FKGKAAIGEGR ASFG QAPTVSRFSSRGPDYMN NRT ADVLKPDILAPGHQ+W
Sbjct: 520 GMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRTAADVLKPDILAPGHQVW 579

Query: 481 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 540
           AAWSPLS +EPLLKGY FAL+SGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK
Sbjct: 580 AAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 639

Query: 541 YDMNGDLIQAEGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           YDMNG+LIQAEG+ IH LYPSTPFDLGAGLV PTNALDPGLVFP+
Sbjct: 640 YDMNGELIQAEGYGIHSLYPSTPFDLGAGLVSPTNALDPGLVFPS 684

BLAST of Csor.00g296810 vs. ExPASy TrEMBL
Match: A0A6J1CLJ5 (subtilisin-like protease SBT2.4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012715 PE=3 SV=1)

HSP 1 Score: 945 bits (2443), Expect = 0.0
Identity = 476/585 (81.37%), Postives = 516/585 (88.21%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVC-SKKTKTSP 60
           +NGFAVHTTPSEA KLR+A GV  VERDRGVRKMTTYTP+FLGVPS  R     K   S 
Sbjct: 99  INGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPSTLRATVGCKRNESS 158

Query: 61  DGEGIVIGFVDSGIDPTHPSFGFDDGY--EDLRLFC---EKGRFFPLSSCNGKIVGARFF 120
            GEGIVIGFVDSGIDPTHPSFGF  G   ++   FC   E+G FF  +SCNGKIVGARFF
Sbjct: 159 GGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSKFCGVCEEGPFFGSNSCNGKIVGARFF 218

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
             GA+AV++LNSSVDFLSPFDAEGHGSHVASIAAGN GVPV VNGFFYG ASG APRARI
Sbjct: 219 CAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFFYGLASGXAPRARI 278

Query: 181 AVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATR 240
           A+YKAVYPT++TLTDVV+AIDQA+IDGVDILALSVGPNEPPE G TFLSIYDIA+LSATR
Sbjct: 279 AIYKAVYPTVATLTDVVAAIDQAIIDGVDILALSVGPNEPPEVGFTFLSIYDIAVLSATR 338

Query: 241 AGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT- 300
           AGILVVQA GNNGPAP+TVVSYSPW+VGVAASGTDRVYPASLLLGNGQ VGG GLSGPT 
Sbjct: 339 AGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLGNGQXVGGTGLSGPTL 398

Query: 301 ---FFLHKLVLAKDAMNQNGTMPSEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLAA 360
              F LHKLVLAKDA+NQN  +P+EECQYP+AFDPN+VQ+SIV+CSFSQGFLNGTS+L A
Sbjct: 399 GSEFLLHKLVLAKDAVNQNVALPTEECQYPQAFDPNIVQNSIVICSFSQGFLNGTSTLTA 458

Query: 361 IIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDDN 420
           I+HTA TLRF+ F L+ANP+YGDFIAEPIPF +PGIL+P+ SDSQ ILKYYE N CKD  
Sbjct: 459 ILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVSDSQVILKYYEENICKDGR 518

Query: 421 GMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQIW 480
           GMV+ FKGKAAIGEGR ASFG QAPTVSRFSSRGPDYMN NRT ADVLKPDILAPGHQ+W
Sbjct: 519 GMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRTAADVLKPDILAPGHQVW 578

Query: 481 AAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 540
           AAWSPLS +EPLLKGY FAL+SGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK
Sbjct: 579 AAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATK 638

Query: 541 YDMNGDLIQAEGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPA 575
           YDMNG+LIQAEG+ IH LYPSTPFDLGAGLV PTNALDPGLVFP+
Sbjct: 639 YDMNGELIQAEGYGIHSLYPSTPFDLGAGLVSPTNALDPGLVFPS 683

BLAST of Csor.00g296810 vs. ExPASy TrEMBL
Match: A0A1S3C9G9 (LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4 OS=Cucumis melo OX=3656 GN=LOC103498334 PE=3 SV=1)

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 463/590 (78.47%), Postives = 508/590 (86.10%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTK---T 60
           +NGFAVHTTPSEA+KLR+ANGVKLVE DRGV+KMTTYTP+FLG+   +           +
Sbjct: 67  LNGFAVHTTPSEASKLREANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGIS 126

Query: 61  SPDGEGIVIGFVDSGIDPTHPSFGFDDGYEDL---RLFCEKGRFFPLSSCNGKIVGARFF 120
              G+GI+IGFVDSGI P HPSF  + G +D     L CE+G  FP   CNGKIV A FF
Sbjct: 127 GAGGDGILIGFVDSGIYPKHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFF 186

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
           S GA+A A LNSS+DFLSPFDAEGHGSHVASIAAGN  VPV V+GFFYG ASG+AP ARI
Sbjct: 187 SAGAQAAAVLNSSIDFLSPFDAEGHGSHVASIAAGNAEVPVIVDGFFYGLASGIAPHARI 246

Query: 181 AVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSATR 240
            VYKAVYPT++TLTDV+SAIDQAVIDGVDILALSVGPNEPPE G TFLSIYDIAILSATR
Sbjct: 247 GVYKAVYPTIATLTDVISAIDQAVIDGVDILALSVGPNEPPEVGFTFLSIYDIAILSATR 306

Query: 241 AGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT- 300
           AGILVVQA GNNGPA ATVVSYSPW++GVAASGTDRVY +SLLLGNGQK+GGVG+SGP+ 
Sbjct: 307 AGILVVQAAGNNGPARATVVSYSPWAIGVAASGTDRVYSSSLLLGNGQKIGGVGMSGPSL 366

Query: 301 ---FFLHKLVLAKDAMNQNGT----MPS--EECQYPEAFDPNLVQSSIVLCSFSQGFLNG 360
              FFLHKLVLAKDA  QN T    +PS  EECQ PEAFDPN+VQ+SIVLCSFSQGFLNG
Sbjct: 367 GSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNG 426

Query: 361 TSSLAAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEAN 420
           TSSLAAIIHTA  L+F+GF LIANPNYGDFIAEPIPF +PGIL+P+ SD+Q ILKYYE N
Sbjct: 427 TSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEEN 486

Query: 421 TCKDDNGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILA 480
           TCKD  GMV EFKGKA IGEGR+ASFG +APTVSRFSSRGPDY+N+NR++ADVLKPDILA
Sbjct: 487 TCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILA 546

Query: 481 PGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAM 540
           PGHQIWAAWSPLS +EPLLKGY+FALMSGTSMAAPHIVG+AALIKQK PSWTPSMIASAM
Sbjct: 547 PGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAM 606

Query: 541 STTATKYDMNGDLIQAEGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFP 574
           STTATKYDMNGDLIQAEGFN+H LYPSTPFD GAGLV PTNALDPGLVFP
Sbjct: 607 STTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFP 656

BLAST of Csor.00g296810 vs. TAIR 10
Match: AT1G62340.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 688.3 bits (1775), Expect = 5.2e-198
Identity = 357/595 (60.00%), Postives = 427/595 (71.76%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +N  AV TT S+A KL +  GVK VE D+GV+ MTTYTP FL +P +             
Sbjct: 105 INAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYTPDFLELPQQVWQKISNEGDRRA 164

Query: 61  GEGIVIGFVDSGIDPTHPSFG-------FDDGYEDLRLF--CEKGRFFPLSSCNGKIVGA 120
           GE IVIGFVD+GI+PTHPSF        +      L     CE G FFP  SCNGKI+ A
Sbjct: 165 GEDIVIGFVDTGINPTHPSFAALDLTNPYSSNLSRLHFSGDCEIGPFFPPGSCNGKIISA 224

Query: 121 RFFSEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPR 180
           RFFS GARA   LNSS+D LSPFDA GHGSHVASIAAGN GVPV V+GFFYG ASGMAPR
Sbjct: 225 RFFSAGARASGALNSSLDILSPFDASGHGSHVASIAAGNAGVPVIVDGFFYGRASGMAPR 284

Query: 181 ARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILS 240
           +RIAVYKA+YP++ TL DV++AIDQA++DGVD+L LSVGP+EPP +  T L I+D+A+L 
Sbjct: 285 SRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSVGPDEPPVDKPTVLGIFDLAMLL 344

Query: 241 ATRAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSG 300
           A +AG+ VVQAVGNNGP+P++V+SYSPW VGVAA  TDR YPA L+L  GQ V GVGLSG
Sbjct: 345 ARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTDRSYPAPLILDGGQTVQGVGLSG 404

Query: 301 PT----FFLHKLVLAKDAMNQNG------TMPSEECQYPEAFDPNLVQSSIVLCSFSQGF 360
           PT       H+LVLAKDA+  NG      T   EECQ PE FDP  V  SIV+C+FS GF
Sbjct: 405 PTLGAPLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSIVICTFSDGF 464

Query: 361 LNGTSSLAAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYY 420
            N  S++ AI  TA TL F+GF LIANP +GD++AEP+ F  PGILIP  S +Q IL+YY
Sbjct: 465 YNQMSTVLAITQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVSAAQIILRYY 524

Query: 421 EANTCKDDNGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPD 480
           E  T +D  G+ ++F  +A IGEGR + F  +AP VSRFSSRGP +++  R+  DVLKPD
Sbjct: 525 EEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPD 584

Query: 481 ILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIA 540
           ILAPGHQIW AWS  S  +P+L G  FA++SGTSMA PHI GI ALIKQ NPSWTP+MIA
Sbjct: 585 ILAPGHQIWGAWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIA 644

Query: 541 SAMSTTATKYDMNGDLIQAEGFNIHRLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
           SA+STTA +YD NG++I AE + + RL+PS  FD GAG V P  ALDPGLV PAG
Sbjct: 645 SAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAG 699

BLAST of Csor.00g296810 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 508.8 bits (1309), Expect = 5.6e-144
Identity = 285/590 (48.31%), Postives = 375/590 (63.56%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGFA H +P +A  LR+A GV+ V++D  VR++TT+TP+FLG+P+   V          
Sbjct: 93  INGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTD--VWPTGGGFDRA 152

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDD--GYEDLRLF---CEKGRFFPLSSCNGKIVGARFFS 120
           GE IVIGFVDSGI P HPSF       Y  L  +   CE+      S CN KIVGA+ F+
Sbjct: 153 GEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFCNRKIVGAQHFA 212

Query: 121 EGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARIA 180
           E A+A    N  +D+ SP D +GHGSH A+IAAGN G+P+ ++G+ +G ASGMAPRARIA
Sbjct: 213 EAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHGYEFGKASGMAPRARIA 272

Query: 181 VYKAVYPTM-STLTDVVSAIDQAVIDGVDILALSVGPNEPP-EEGLTFLSIYDIAILSAT 240
           VYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TFL+ +D  +L A 
Sbjct: 273 VYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTTKTTFLNPFDATLLGAV 332

Query: 241 RAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT 300
           +AG+ V QA GN GP P T+VSYSPW   VAA+  DR Y   L LGNG+ + G+GLS PT
Sbjct: 333 KAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNGKMLAGMGLSPPT 392

Query: 301 --FFLHKLVLAKDAMNQNGTMP--SEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSLA 360
               L+ LV A D +  +        +CQ PE F+  LV+ +I+LC +S  F+ GT+S+ 
Sbjct: 393 RPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNKKLVEGNILLCGYSFNFVVGTASIK 452

Query: 361 AIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKDD 420
            ++ TA  L   GF L+          +P+P  +PGILI + S S  ++ YY A+T +D 
Sbjct: 453 KVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDVSKSMDLIDYYNASTSRDW 512

Query: 421 NGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQI 480
            G V  FK + +IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ I
Sbjct: 513 TGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLI 572

Query: 481 WAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTAT 540
           WAAW P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T
Sbjct: 573 WAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTST 632

Query: 541 KYDMNGDLIQAEGFN---IHRLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
             D  G L+QA+ ++      L  +TPFD G+G V P+ ALDPGL+F AG
Sbjct: 633 VIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAG 680

BLAST of Csor.00g296810 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 498.0 bits (1281), Expect = 9.9e-141
Identity = 280/591 (47.38%), Postives = 370/591 (62.61%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGFA H +P +A  LR+A GVK V+RD  VRK+TT+TP+FLG+P+   V          
Sbjct: 93  INGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTD--VWPTGGGYDRA 152

Query: 61  GEGIVIGFVDSGIDPTHPSFGFD------DGYEDLRLFCEKGRFFPLSSCNGKIVGARFF 120
           GE IVIGF+DSGI P HPSF           +   +  CE+     +S CNGKI+GA+ F
Sbjct: 153 GEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISFCNGKIIGAQHF 212

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
           +E A+A    N  +DF SP D +GHGSH A+IAAGN G+PV ++G+ +G ASGMAPRARI
Sbjct: 213 AEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNNGIPVRMHGYEFGKASGMAPRARI 272

Query: 181 AVYKAVYPTM-STLTDVVSAIDQAVIDGVDILALSVGPNEPP-EEGLTFLSIYDIAILSA 240
           AVYKA+Y      + DVV+AIDQAV DGVDIL+LSVGPN PP     TFL+ +D  +L A
Sbjct: 273 AVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATTKTTFLNPFDATLLGA 332

Query: 241 TRAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGP 300
            +AG+ V QA GN GP P T+VSYSPW   VAA+  DR Y   L LGNG+ + G+GLS  
Sbjct: 333 VKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNGKMLAGIGLSPS 392

Query: 301 T--FFLHKLVLAKDAMNQNGTMP--SEECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSL 360
           T     +K+V A D +  +  M     +CQ PE  +  LV+ +I+LC +S  F+ G++S+
Sbjct: 393 TRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLNKKLVEGNILLCGYSFNFVAGSASI 452

Query: 361 AAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKD 420
             +  TA  L   GF L+          +P+P  +PGILI + S S  ++ YY   T +D
Sbjct: 453 KKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIPGILITDVSKSMDLIDYYNVTTSRD 512

Query: 421 DNGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGHQ 480
             G V +FK + +IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  
Sbjct: 513 WMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSL 572

Query: 481 IWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTA 540
           IW+AWS     E    G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+
Sbjct: 573 IWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTS 632

Query: 541 TKYDMNGDLIQAEGFN---IHRLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
           T  D  G  +QA+ ++      L  +TPFD G+G V P+ ALDPGL+F AG
Sbjct: 633 TVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAG 681

BLAST of Csor.00g296810 vs. TAIR 10
Match: AT1G30600.1 (Subtilase family protein )

HSP 1 Score: 468.0 bits (1203), Expect = 1.1e-131
Identity = 268/592 (45.27%), Postives = 366/592 (61.82%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGF+   T  +A +L     V+ V  D  V K TT+TP+FLG+P  + +    ++ +  
Sbjct: 105 INGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYA-- 164

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRL------FCEKGRFFPLSSCNGKIVGARFF 120
           GEG+VIGF+D+GIDPTHPSF          +       CE    FP  SCN K++GAR F
Sbjct: 165 GEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHF 224

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
           +E A +   LNSS D  SPFD EGHG+H AS+AAGN G+PV V G   G ASGMAPRA I
Sbjct: 225 AESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHI 284

Query: 181 AVYKAVYPTMSTL-TDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSAT 240
           A+YKA+Y        D+++AIDQA  DGVDI+ LS+ PN  P    TF +  D+A+LSA 
Sbjct: 285 AIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAV 344

Query: 241 RAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT 300
           +AGI VVQA GN GPAP ++ S+SPW   V A+  DRVY  S++LGN   + GVGL+  T
Sbjct: 345 KAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT 404

Query: 301 FFLHKLVLAKDAMNQNGTMPSE-----ECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSSL 360
             +HKLVLA  A+ +NGT   +     ECQ   +FD  LVQ  I++CS++  F+ G S++
Sbjct: 405 RIMHKLVLATHAL-RNGTTVMDAIYVGECQDSSSFDQKLVQGKILVCSYTVRFILGVSTI 464

Query: 361 AAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCKD 420
              + TA  L   G     +P+   F     P  +PGILI +  DSQA+L+YY ++  ++
Sbjct: 465 KQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILISSPQDSQALLRYYNSSLLRE 524

Query: 421 D-NGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPGH 480
           + +G +      A I  G   ++G  AP V  FS+RGPD  + +   AD++KP+++APG+
Sbjct: 525 NGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDPEDDSFVDADIMKPNLVAPGN 584

Query: 481 QIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTT 540
            IW AWSPL +     +G +FA+ SGTSM+APH+ GIAALIKQK P +TP+ IASA+STT
Sbjct: 585 AIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAALIKQKFPHFTPAAIASALSTT 644

Query: 541 ATKYDMNGDLIQAEGFNIH---RLYPSTPFDLGAGLVKPTNALDPGLVFPAG 577
           A+  D  G+ I A+   ++      P+TPFD+G+G V  T ALDPGL+F  G
Sbjct: 645 ASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNATAALDPGLIFDIG 693

BLAST of Csor.00g296810 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 467.2 bits (1201), Expect = 1.9e-131
Identity = 265/590 (44.92%), Postives = 359/590 (60.85%), Query Frame = 0

Query: 1   MNGFAVHTTPSEAAKLRQANGVKLVERDRGVRKMTTYTPKFLGVPSKSRVCSKKTKTSPD 60
           +NGFA+     +A KL     V  +  D  VR  TTYTP+F+G+P  + V  K+      
Sbjct: 113 INGFALFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWV--KEGGFEIA 172

Query: 61  GEGIVIGFVDSGIDPTHPSFGFDDGYEDLRL------FCEKGRFFPLSSCNGKIVGARFF 120
           GEG++IGF+D+GIDP HPSF  +D      +       CE    FP  SCN K++GAR F
Sbjct: 173 GEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGARHF 232

Query: 121 SEGARAVAKLNSSVDFLSPFDAEGHGSHVASIAAGNGGVPVYVNGFFYGFASGMAPRARI 180
           ++ A      NSS D+ SPFD +GHG+H AS+AAGN GVPV V+   +G+ASG+APRA I
Sbjct: 233 AQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPRAFI 292

Query: 181 AVYKAVYPTMSTL-TDVVSAIDQAVIDGVDILALSVGPNEPPEEGLTFLSIYDIAILSAT 240
           +VYKA+Y +      DVV+AIDQA  DGVDIL+LS+ PN  P    TF +  D+A+LSA 
Sbjct: 293 SVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPPGVATFFNPIDMALLSAV 352

Query: 241 RAGILVVQAVGNNGPAPATVVSYSPWSVGVAASGTDRVYPASLLLGNGQKVGGVGLSGPT 300
           +AGI VVQA GN GPAP T+ S+SPW   V AS  DRVY  SL LGN   + G+G + PT
Sbjct: 353 KAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFAIPT 412

Query: 301 FF--LHKLVLAKDAMNQNGTMPSE----ECQYPEAFDPNLVQSSIVLCSFSQGFLNGTSS 360
               ++K++ A  A+N + ++  +    ECQ  E FD + V   +++CS+S  F+ G S+
Sbjct: 413 DSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLST 472

Query: 361 LAAIIHTATTLRFIGFALIANPNYGDFIAEPIPFGLPGILIPNASDSQAILKYYEANTCK 420
           +   +  A  L   G     +P    F   P P  +PGI+IP+  DS+ +LKYY ++  +
Sbjct: 473 IKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQR 532

Query: 421 D-DNGMVSEFKGKAAIGEGRVASFGSQAPTVSRFSSRGPDYMNMNRTVADVLKPDILAPG 480
           D     +  F   AAI  G  A+F ++AP V  +S+RGPD  + +   ADVLKP+++APG
Sbjct: 533 DVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPG 592

Query: 481 HQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMST 540
           + IW AWS  S      +G +FA+MSGTSMAAPH+ G+AALIKQ  P +TPS I+SA+ST
Sbjct: 593 NSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALST 652

Query: 541 TATKYDMNGDLIQAEGFNIH---RLYPSTPFDLGAGLVKPTNALDPGLVF 574
           TA   D  G  I A+    +    LY +TP D+G+G V  T ALDPGLVF
Sbjct: 653 TALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVF 700