Csor.00g244530 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g244530
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionAuxin-responsive GH3 family protein
LocationCsor_Chr16: 1613949 .. 1615916 (-)
RNA-Seq ExpressionCsor.00g244530
SyntenyCsor.00g244530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTCGATTCTTCTCTCTCTTCACCTCTCGGCCCTCCCGCCTGCGAAAAGGACGCCAAGGCTCTTCAATTCATCGAGCAAATGACTCGCAACGCCGACGCTGTCCAGCAGACAGTCCTCGCCGAAATCTTGACCAGAAACGCTGCCACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCCACCGATCCCCACACCTTCAAAGAAAAACTCCCGGTCATCACCTATGAGGATCTCGAGCCAGACATCCAACGGATTGCCAATGGAGATCGCTCTCCTATCCTCTCCTCCCATCCAATCTCCGAGTTCCTCACCAGGTGAATTACACTGAAATCTCTTCATCGGAGCAATTAGACATTTAGAATAGTTGAATCGATTTTCTGATGCTGTGGTTTTTGCAGTTCTGGTACTTCTGCCGGTGAGAGGAAGCTTATGCCGACGATTAAGGAGGAGCTGGATCGTCGTCAACTTCTGTACAGTCTACTCATGCCAGTTATGAACCTGTAAGAGAAAGCAACAATATCAATGGCGAACTTGAATTATTTAAATTAAAAGCGCGTAAAATTTAATATATTCATGGATTCTGTTTTGGACAGCTATGTGCCTGGGCTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCTGTTTTGACCAGTTACTACAAGAGCGACCACTTCAAGACCCGACCCTACGACCCGTTTGTTGTCTACACGAGCCCTAACGAGGCCATCCTCTGCCCCGATTCCTTCCAGAGCATGTACACGCAGATGCTCTGCGGTCTTCTTCAACGCCATCAAGTCCTCCGCCTCGGCGCCATCTTCGCCTCCGGCCTTCTCCGAGCCATTCGATTCCTTCAATTGAACTGGCAGGACCTTGCTAACGATATCCGGAACGGAACGTTGAATTCGAAAATCACCGATCCGTCGTTGAGAGATTGCATTGAGAAGATCCTTAAGCCGGACGCGGAATTGGCGGATTTCGTTTCGGATGAATGTTCGAAGGAGGATTGGGAGGGAATCGTGACGAGGATTTGGCCGAAGACGAAATACCTCGACGTGATCGTCACTGGAGCGATGGCGCAGTACATTCCAACGCTTGATTACTACAGCGGCGGATTGCCTCTGGCTTGCACGATGTACGCATCTTCCGAATGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCATCGGAGGTTTCCTACACTATAATGCCAAACATGGCGTACTTCGAGTTTCTCCCTCACGAGCCAAACACCGAAAGCGATTCGCCGCCGAAACTCGTCGACCTGGTAGACGTGGAGGTCGGAAAAGAATACGAACTAGTAATCACAACATACGCCGGACTTTACCGGTACCGAGTAGGCGACATACTCCGAGTTACCGGATTCCACAACGCAGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTCCTGAGCATCGACTCAGACAAAACGGACGAAGCGGAGTTGCAGAAGGCAGTGGAGAATGCATCAAAGCTGCTGAAGGAATGGAAGGCGAGCGTGGTTGAGTACACGAGTTACGCAGAGACGAAGACGATTCCAGGGCACTACGTGATATACTGGGAACTGATGGTGAAGGAAGCGGAAGGGAAATGGCCGAGCAAGGAGGTAATGGAGGACTGCTGCTTGGCAATGGAGGAATCATTGAACTCGGTGTACCGACAAGGGCGAGTTGCAGACCATTCGATCGGAGCTTTAGAAATCAGAGTGGTGAAGAACGGAACATTCGAAGAACTGATGGATTACGCAATTTCCAGAGGAGCCTCTATCAACCAGTATAAGGTGCCGAGGTGTGTGAACTTTACTCCCATTATGGAACTTCTCGATTCCAGAGTAGTGTCCGCACACTTCAGCCCTGCCCTGCCACGTTGGACTCCAGCTAGGACACGCTAA

mRNA sequence

ATGGCTGTCGATTCTTCTCTCTCTTCACCTCTCGGCCCTCCCGCCTGCGAAAAGGACGCCAAGGCTCTTCAATTCATCGAGCAAATGACTCGCAACGCCGACGCTGTCCAGCAGACAGTCCTCGCCGAAATCTTGACCAGAAACGCTGCCACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCCACCGATCCCCACACCTTCAAAGAAAAACTCCCGGTCATCACCTATGAGGATCTCGAGCCAGACATCCAACGGATTGCCAATGGAGATCGCTCTCCTATCCTCTCCTCCCATCCAATCTCCGAGTTCCTCACCAGTTCTGGTACTTCTGCCGGTGAGAGGAAGCTTATGCCGACGATTAAGGAGGAGCTGGATCGTCGTCAACTTCTGTACAGTCTACTCATGCCAGTTATGAACCTCTATGTGCCTGGGCTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCTGTTTTGACCAGTTACTACAAGAGCGACCACTTCAAGACCCGACCCTACGACCCGTTTGTTGTCTACACGAGCCCTAACGAGGCCATCCTCTGCCCCGATTCCTTCCAGAGCATGTACACGCAGATGCTCTGCGGTCTTCTTCAACGCCATCAAGTCCTCCGCCTCGGCGCCATCTTCGCCTCCGGCCTTCTCCGAGCCATTCGATTCCTTCAATTGAACTGGCAGGACCTTGCTAACGATATCCGGAACGGAACGTTGAATTCGAAAATCACCGATCCGTCGTTGAGAGATTGCATTGAGAAGATCCTTAAGCCGGACGCGGAATTGGCGGATTTCGTTTCGGATGAATGTTCGAAGGAGGATTGGGAGGGAATCGTGACGAGGATTTGGCCGAAGACGAAATACCTCGACGTGATCGTCACTGGAGCGATGGCGCAGTACATTCCAACGCTTGATTACTACAGCGGCGGATTGCCTCTGGCTTGCACGATGTACGCATCTTCCGAATGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCATCGGAGGTTTCCTACACTATAATGCCAAACATGGCGTACTTCGAGTTTCTCCCTCACGAGCCAAACACCGAAAGCGATTCGCCGCCGAAACTCGTCGACCTGGTAGACGTGGAGGTCGGAAAAGAATACGAACTAGTAATCACAACATACGCCGGACTTTACCGGTACCGAGTAGGCGACATACTCCGAGTTACCGGATTCCACAACGCAGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTCCTGAGCATCGACTCAGACAAAACGGACGAAGCGGAGTTGCAGAAGGCAGTGGAGAATGCATCAAAGCTGCTGAAGGAATGGAAGGCGAGCGTGGTTGAGTACACGAGTTACGCAGAGACGAAGACGATTCCAGGGCACTACGTGATATACTGGGAACTGATGGTGAAGGAAGCGGAAGGGAAATGGCCGAGCAAGGAGGTAATGGAGGACTGCTGCTTGGCAATGGAGGAATCATTGAACTCGGTGTACCGACAAGGGCGAGTTGCAGACCATTCGATCGGAGCTTTAGAAATCAGAGTGGTGAAGAACGGAACATTCGAAGAACTGATGGATTACGCAATTTCCAGAGGAGCCTCTATCAACCAGTATAAGGTGCCGAGGTGTGTGAACTTTACTCCCATTATGGAACTTCTCGATTCCAGAGTAGTGTCCGCACACTTCAGCCCTGCCCTGCCACGTTGGACTCCAGCTAGGACACGCTAA

Coding sequence (CDS)

ATGGCTGTCGATTCTTCTCTCTCTTCACCTCTCGGCCCTCCCGCCTGCGAAAAGGACGCCAAGGCTCTTCAATTCATCGAGCAAATGACTCGCAACGCCGACGCTGTCCAGCAGACAGTCCTCGCCGAAATCTTGACCAGAAACGCTGCCACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCCACCGATCCCCACACCTTCAAAGAAAAACTCCCGGTCATCACCTATGAGGATCTCGAGCCAGACATCCAACGGATTGCCAATGGAGATCGCTCTCCTATCCTCTCCTCCCATCCAATCTCCGAGTTCCTCACCAGTTCTGGTACTTCTGCCGGTGAGAGGAAGCTTATGCCGACGATTAAGGAGGAGCTGGATCGTCGTCAACTTCTGTACAGTCTACTCATGCCAGTTATGAACCTCTATGTGCCTGGGCTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCTGTTTTGACCAGTTACTACAAGAGCGACCACTTCAAGACCCGACCCTACGACCCGTTTGTTGTCTACACGAGCCCTAACGAGGCCATCCTCTGCCCCGATTCCTTCCAGAGCATGTACACGCAGATGCTCTGCGGTCTTCTTCAACGCCATCAAGTCCTCCGCCTCGGCGCCATCTTCGCCTCCGGCCTTCTCCGAGCCATTCGATTCCTTCAATTGAACTGGCAGGACCTTGCTAACGATATCCGGAACGGAACGTTGAATTCGAAAATCACCGATCCGTCGTTGAGAGATTGCATTGAGAAGATCCTTAAGCCGGACGCGGAATTGGCGGATTTCGTTTCGGATGAATGTTCGAAGGAGGATTGGGAGGGAATCGTGACGAGGATTTGGCCGAAGACGAAATACCTCGACGTGATCGTCACTGGAGCGATGGCGCAGTACATTCCAACGCTTGATTACTACAGCGGCGGATTGCCTCTGGCTTGCACGATGTACGCATCTTCCGAATGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCATCGGAGGTTTCCTACACTATAATGCCAAACATGGCGTACTTCGAGTTTCTCCCTCACGAGCCAAACACCGAAAGCGATTCGCCGCCGAAACTCGTCGACCTGGTAGACGTGGAGGTCGGAAAAGAATACGAACTAGTAATCACAACATACGCCGGACTTTACCGGTACCGAGTAGGCGACATACTCCGAGTTACCGGATTCCACAACGCAGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTCCTGAGCATCGACTCAGACAAAACGGACGAAGCGGAGTTGCAGAAGGCAGTGGAGAATGCATCAAAGCTGCTGAAGGAATGGAAGGCGAGCGTGGTTGAGTACACGAGTTACGCAGAGACGAAGACGATTCCAGGGCACTACGTGATATACTGGGAACTGATGGTGAAGGAAGCGGAAGGGAAATGGCCGAGCAAGGAGGTAATGGAGGACTGCTGCTTGGCAATGGAGGAATCATTGAACTCGGTGTACCGACAAGGGCGAGTTGCAGACCATTCGATCGGAGCTTTAGAAATCAGAGTGGTGAAGAACGGAACATTCGAAGAACTGATGGATTACGCAATTTCCAGAGGAGCCTCTATCAACCAGTATAAGGTGCCGAGGTGTGTGAACTTTACTCCCATTATGGAACTTCTCGATTCCAGAGTAGTGTCCGCACACTTCAGCCCTGCCCTGCCACGTTGGACTCCAGCTAGGACACGCTAA

Protein sequence

MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLDGATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRFLQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIPGHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR
Homology
BLAST of Csor.00g244530 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 5.4e-280
Identity = 476/597 (79.73%), Postives = 531/597 (88.94%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDS+LSSPLGPPACEKDAKAL+FIE+MTRNAD VQ+ +LAEIL RNA TEYLRR++L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD  TFK K+PVITYEDL+P+IQRIA+GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HF++RPYDP+ VYTSPNEAILCPDSFQSMYTQMLCGLL R  VLR+GA+FASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQL+W   A+DI  G L+S+ITDPS+R C+  ILKPD  LA+F+  EC  ++WE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLL-KEWKASVVEYTSYAETKTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYA+T TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKN 540
           PGHYV+YWEL+V++   + PS E +  CCL MEESLNSVYRQ RVAD+S+G LEIRVV+N
Sbjct: 481 PGHYVLYWELLVRDG-ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 597
           GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LP WTP R R
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of Csor.00g244530 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 1.6e-263
Identity = 458/599 (76.46%), Postives = 518/599 (86.48%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           M VDS+L SP+      KD KAL+FIE+MTRN D VQ+ V+ EIL+RN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+P+IQRIANGDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKS+
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
            FK RP DP+ VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGA+FASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKED-WEGIVT 300
           LQ NW++LA+DI  GTL+S+I+DP++++ + KIL KPD ELADF++  C +++ WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLP-HEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRV 420
           TIMPNMAYFEFLP HE  TE     +LV+L DVEVGKEYELVITTYAGL RYRVGDIL+V
Sbjct: 361 TIMPNMAYFEFLPHHEVPTEKS---ELVELADVEVGKEYELVITTYAGLNRYRVGDILQV 420

Query: 421 TGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETK 480
           TGF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E    V+EYTSYAETK
Sbjct: 421 TGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETK 480

Query: 481 TIPGHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVV 540
           TIPGHYVIYWEL+VK+     P+ EVM  CCL MEESLNSVYRQ RVAD SIG LEIRVV
Sbjct: 481 TIPGHYVIYWELLVKDQTNP-PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVV 540

Query: 541 KNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 597
           KNGTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALP W+P R R
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

BLAST of Csor.00g244530 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 888.3 bits (2294), Expect = 4.9e-257
Identity = 441/591 (74.62%), Postives = 494/591 (83.59%), Query Frame = 0

Query: 5   SSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLDGATD 64
           ++L +P      E D + L+FI++MT N DAVQ+ VL EIL RNA TEYL +  LDGATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+ K+PV++Y+DL+P IQRIANGDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKSDHFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRFLQLN 244
           RPYDP+  YTSP  AILC D+FQSMY QM+CGL QR+ VLRLGA+FASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKT 304
           W+ LA+DI +G L  ++TDPS+R+ +  IL PD ELA  +  ECSK DW GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLP-HEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP  E    S    +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+   ASVVEYTS A TK IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNGTFE 544
           VIYWEL+ K A       + +  CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GTFE
Sbjct: 492 VIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFE 551

Query: 545 ELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPAR 595
           ELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALP WTPAR
Sbjct: 552 ELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPAR 602

BLAST of Csor.00g244530 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 4.9e-257
Identity = 441/591 (74.62%), Postives = 494/591 (83.59%), Query Frame = 0

Query: 5   SSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLDGATD 64
           ++L +P      E D + L+FI++MT N DAVQ+ VL EIL RNA TEYL +  LDGATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+ K+PV++Y+DL+P IQRIANGDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKSDHFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRFLQLN 244
           RPYDP+  YTSP  AILC D+FQSMY QM+CGL QR+ VLRLGA+FASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKT 304
           W+ LA+DI +G L  ++TDPS+R+ +  IL PD ELA  +  ECSK DW GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLP-HEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP  E    S    +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+   ASVVEYTS A TK IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNGTFE 544
           VIYWEL+ K A       + +  CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GTFE
Sbjct: 492 VIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFE 551

Query: 545 ELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPAR 595
           ELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALP WTPAR
Sbjct: 552 ELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPAR 602

BLAST of Csor.00g244530 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 2.7e-255
Identity = 432/589 (73.34%), Postives = 502/589 (85.23%), Query Frame = 0

Query: 15  ACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLDGATDPHTFKEKLPV 74
           ACE+DA+ L+FIE+MTR  DAVQ+ VLA IL RN   EYLRR+ ++G TD   FK ++PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 75  ITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSL 134
           +TYEDL P+I+RIANGDRS I+SSHPI+EFLTSSGTSAGERKLMPTI++ELDRRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 135 LMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFVVYT 194
           LMPVMNLYVPGLDKGKGLYFLF+KSET+TPGGL ARPVLTSYYKSDHFK RP+DP+ VYT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 195 SPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRFLQLNWQDLANDIRN 254
           SP  AILC D+FQSMY QMLCGL+ R +VLR+GA+FASGLLRAIRFLQL+W++LA+DIR 
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 255 GTLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKEDWEGIVTRIWPKTKYLDVIVTG 314
           GTL++K+T+PS+RD + ++L  PDAELA FV  EC K+ WEGI+TR+WP TKYLDVIVTG
Sbjct: 265 GTLSAKVTEPSIRDAVAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVTG 324

Query: 315 AMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHE 374
           AMAQYIPTL +YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM YFE +PH+
Sbjct: 325 AMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPHD 384

Query: 375 PNT----ESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHF 434
           P+         PP+LVDL D EVG+EYELVITTYAGL RYRVGDIL+VTGFHNAAPQF F
Sbjct: 385 PDAPPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQFRF 444

Query: 435 VRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIPGHYVIYWEL 494
           VRRKNV+LSIDSDKTDEAELQ AVE AS LL  + AS+VEYTS A+  TIPGHYV+YWEL
Sbjct: 445 VRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVYWEL 504

Query: 495 MVKEAEGKWP------SKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNGTFE 554
           MV+E  G WP       + V E CCL MEE+LN+VYRQGR  + +IG LEIRVV+ GTFE
Sbjct: 505 MVREG-GAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGTFE 564

Query: 555 ELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTP 593
           E+MDYAISRGASINQYK PRCV+F PI+ELL+SRV+S HFSPA P+++P
Sbjct: 565 EVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSP 611

BLAST of Csor.00g244530 vs. NCBI nr
Match: KAG6576928.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014954.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1202 bits (3110), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of Csor.00g244530 vs. NCBI nr
Match: XP_022922958.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1200 bits (3105), Expect = 0.0
Identity = 594/596 (99.66%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKEAEGKWPSKEVME+CCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of Csor.00g244530 vs. NCBI nr
Match: XP_023552180.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1197 bits (3096), Expect = 0.0
Identity = 591/596 (99.16%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLANDIRNGTLN+KITDPSLRDCI+KILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIDKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKEAEGKWPSKEVME+CCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSR+VSAHFSPALPRWTPARTR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRLVSAHFSPALPRWTPARTR 596

BLAST of Csor.00g244530 vs. NCBI nr
Match: XP_022985331.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima])

HSP 1 Score: 1194 bits (3090), Expect = 0.0
Identity = 590/596 (98.99%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TDPHTFK KLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GTTDPHTFKGKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLANDIRNGTLN+KITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKEAEGKWPSKEVME+CCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRV+SAHFSPALPRWTPARTR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVISAHFSPALPRWTPARTR 596

BLAST of Csor.00g244530 vs. NCBI nr
Match: XP_038877583.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida])

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 569/598 (95.15%), Postives = 584/598 (97.66%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAV SSLSSPLGPPACE DAK LQFI+QMTRNADAVQ TVLAEIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLA DIRNGTLNS+ITDPSLRDCIEKILKPDAELADF+SDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFLSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE AS+LLKEWK SVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASELLKEWKTSVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEA--EGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVK 540
           GHYVIYWELMVKEA  EGKWPSKEVME CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK
Sbjct: 481 GHYVIYWELMVKEATEEGKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVK 540

Query: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           +GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARTR
Sbjct: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARTR 598

BLAST of Csor.00g244530 vs. ExPASy TrEMBL
Match: A0A6J1E8A0 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111430783 PE=3 SV=1)

HSP 1 Score: 1200 bits (3105), Expect = 0.0
Identity = 594/596 (99.66%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKEAEGKWPSKEVME+CCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of Csor.00g244530 vs. ExPASy TrEMBL
Match: A0A6J1J7V1 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111483372 PE=3 SV=1)

HSP 1 Score: 1194 bits (3090), Expect = 0.0
Identity = 590/596 (98.99%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TDPHTFK KLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GTTDPHTFKGKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNWQDLANDIRNGTLN+KITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKEAEGKWPSKEVME+CCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRV+SAHFSPALPRWTPARTR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVISAHFSPALPRWTPARTR 596

BLAST of Csor.00g244530 vs. ExPASy TrEMBL
Match: A0A6J1IUJ6 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111479427 PE=3 SV=1)

HSP 1 Score: 1132 bits (2927), Expect = 0.0
Identity = 559/596 (93.79%), Postives = 579/596 (97.15%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQ+TVLAEILTRNA+TEYLRRY L 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK+KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDP++VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNW DLANDIR G L+SKITDPSLRDCIEKILKPD E ADF+S+ECSKE+WEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSSKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ SDS PKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGSDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKE K SVVEYTSYA+T TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKE EGKWPSKE ME CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+G
Sbjct: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPAR R
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 596

BLAST of Csor.00g244530 vs. ExPASy TrEMBL
Match: A0A6J1FTR9 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111448040 PE=3 SV=1)

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 558/596 (93.62%), Postives = 580/596 (97.32%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQ+TVLAEILTRNA+TEYLRRY L 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK+KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDP++VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNW DLANDIR G L++KITDPSLRDCIEKILKPD E ADF+S+ECSKE+WEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DS PKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKE K SVVEYTSYA+T TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540
           GHYVIYWELMVKE EGKWPSKE ME CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+G
Sbjct: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPAR R
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 596

BLAST of Csor.00g244530 vs. ExPASy TrEMBL
Match: A0A5A7T148 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold379G001170 PE=3 SV=1)

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 558/599 (93.16%), Postives = 579/599 (96.66%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAV SSLSSPLGPPACE DAK LQFI++MTRNADAVQ TVLAEIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQLNW DLA DIRNGTLNS+ITDPSLRDCI KILKPD++LADFVSDECSKEDWEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP EPN+ SDS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKEWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEA-----EGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIR 540
           GHYVIYWELMVKEA     +GKWPSKEVME+CCL MEES+NSVYRQGRVADHSIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPAR 594
           VVK+GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALP WTPAR
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPAR 599

BLAST of Csor.00g244530 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 964.5 bits (2492), Expect = 3.8e-281
Identity = 476/597 (79.73%), Postives = 531/597 (88.94%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           MAVDS+LSSPLGPPACEKDAKAL+FIE+MTRNAD VQ+ +LAEIL RNA TEYLRR++L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD  TFK K+PVITYEDL+P+IQRIA+GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
           HF++RPYDP+ VYTSPNEAILCPDSFQSMYTQMLCGLL R  VLR+GA+FASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300
           LQL+W   A+DI  G L+S+ITDPS+R C+  ILKPD  LA+F+  EC  ++WE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLL-KEWKASVVEYTSYAETKTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYA+T TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKN 540
           PGHYV+YWEL+V++   + PS E +  CCL MEESLNSVYRQ RVAD+S+G LEIRVV+N
Sbjct: 481 PGHYVLYWELLVRDG-ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 597
           GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LP WTP R R
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of Csor.00g244530 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 929.1 bits (2400), Expect = 1.8e-270
Identity = 463/603 (76.78%), Postives = 521/603 (86.40%), Query Frame = 0

Query: 1   MAVDSSLSSPL-GPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSL 60
           MAVDS L S +      EKD KAL+FIE+MTRN D+VQ+ VL EILTRN+ TEYL+R+ L
Sbjct: 1   MAVDSPLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDL 60

Query: 61  DGATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
           DG  D  TFK K+PV+TYEDL+P+IQRI+NGD SPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  DGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSE++T GGL ARPVLTSYYKS
Sbjct: 121 TIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKS 180

Query: 181 DHFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIR 240
           DHFK RPYDP+ VYTSPNEAILC DS QSMY QMLCGLL RH+VLRLGA+FASGLLRAI 
Sbjct: 181 DHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAIS 240

Query: 241 FLQLNWQDLANDIRNGTLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKEDWEGIVT 300
           FLQ NW++LA DI  GTL+S+I DP++++ + KIL KPD ELA+F+   CS+E+WEGI+T
Sbjct: 241 FLQNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNTE-----SDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGD 420
           TIMPNMAYFEFLPH  + +     S     LV+L +VEVGKEYELVITTYAGLYRYRVGD
Sbjct: 361 TIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGD 420

Query: 421 ILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSY 480
           ILRVTGFHN+APQF F+RRKNV+LS++SDKTDEAELQKAVENAS+L  E    V+EYTSY
Sbjct: 421 ILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSY 480

Query: 481 AETKTIPGHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALE 540
           AETKTIPGHYVIYWEL+ ++      S+EVM  CCL MEESLNSVYRQ RVAD SIG LE
Sbjct: 481 AETKTIPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPA 597
           IRVV+NGTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVSAHFSP+LP W+P 
Sbjct: 541 IRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPE 600

BLAST of Csor.00g244530 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 909.8 bits (2350), Expect = 1.1e-264
Identity = 458/599 (76.46%), Postives = 518/599 (86.48%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60
           M VDS+L SP+      KD KAL+FIE+MTRN D VQ+ V+ EIL+RN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+P+IQRIANGDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKS+
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240
            FK RP DP+ VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGA+FASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKED-WEGIVT 300
           LQ NW++LA+DI  GTL+S+I+DP++++ + KIL KPD ELADF++  C +++ WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLP-HEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRV 420
           TIMPNMAYFEFLP HE  TE     +LV+L DVEVGKEYELVITTYAGL RYRVGDIL+V
Sbjct: 361 TIMPNMAYFEFLPHHEVPTEKS---ELVELADVEVGKEYELVITTYAGLNRYRVGDILQV 420

Query: 421 TGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETK 480
           TGF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E    V+EYTSYAETK
Sbjct: 421 TGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETK 480

Query: 481 TIPGHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVV 540
           TIPGHYVIYWEL+VK+     P+ EVM  CCL MEESLNSVYRQ RVAD SIG LEIRVV
Sbjct: 481 TIPGHYVIYWELLVKDQTNP-PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVV 540

Query: 541 KNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 597
           KNGTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALP W+P R R
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

BLAST of Csor.00g244530 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 863.2 bits (2229), Expect = 1.2e-250
Identity = 432/597 (72.36%), Postives = 504/597 (84.42%), Query Frame = 0

Query: 1   MAVDSSLSSPL-GPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSL 60
           MAVDS L S +  P   E + KAL+FIE++TRN D+VQ+ VL EIL+RN+ TEYL+R+ L
Sbjct: 1   MAVDSLLQSGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDL 60

Query: 61  DGATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
           +GA D  +FK K+PV+ YEDL+ DIQRI+NGDRSPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  NGAVDRKSFKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+++RRQLL +LLMPVMNLYVPGLDKGKGLYFLFVKSE+ T GGL ARP LTSYYKS
Sbjct: 121 TIEEDINRRQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKS 180

Query: 181 DHFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIR 240
           D+F+T   D   VYTSP EAILC DS QSMYTQMLCGLL RH+V RLGA+F SGLLRAI 
Sbjct: 181 DYFRTSDSDS--VYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAIS 240

Query: 241 FLQLNWQDLANDIRNGTLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKEDWEGIVT 300
           FLQ NW++L+ DI  GTL+SKI D +++  +  IL KPD ELA+F+   CS+E+WEGI+T
Sbjct: 241 FLQNNWKELSQDISTGTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMA+YIP L+YYSGGLP+A  +YASSE YFG+NLNPMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNTESD-SPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRV 420
           TI PNMAYFEFLPH  + +       LV+L DVEVGKEYELVITTYAGLYRYRVGDILRV
Sbjct: 361 TIFPNMAYFEFLPHNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRV 420

Query: 421 TGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETK 480
           TGFHN+APQF F+RR+NV+LSI+SDKTDEA+LQKAVENAS+LL E    V+EYTSYA+TK
Sbjct: 421 TGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTK 480

Query: 481 TIPGHYVIYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVV 540
           TIPGHYVIYWEL+ ++     PS EVM  CCL MEESLN+VYRQ RV+D SIG LEIRVV
Sbjct: 481 TIPGHYVIYWELLSRDQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVV 540

Query: 541 KNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPAR 595
           +NGTFEELMD++ISRG+SINQYKVPRCV+ TPIM+LLDSRVVSAHFSP+LP W+P R
Sbjct: 541 QNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPER 595

BLAST of Csor.00g244530 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 825.9 bits (2132), Expect = 2.1e-239
Identity = 404/588 (68.71%), Postives = 482/588 (81.97%), Query Frame = 0

Query: 17  EKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLDGATDPHTFKEKLPVIT 76
           EK+   LQFIE +T NAD VQ+ VL EIL+RNA  EYL+R+ L+G TD  TFK  +PV+T
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 77  YEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136
           YED++P+I RIANGD+S +L S+PISEFLTSSGTS GERKLMPTI+EELDRR LLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 137 PVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFVVYTSP 196
           PVM+ +VPGLDKGKG+YFLF+KSE++TPGGL ARPVLTSYYKS HFK RPYDP+  YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 197 NEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRFLQLNWQDLANDIRNGT 256
           N+ ILC DS+QSMY+QMLCGL Q  +VLR+GA+FASG +RAI+FL+ +W +LA DIR GT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 257 LNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRIWPKTKYLDVIVTGAMA 316
           L+S+ITD S+R+ + +ILKPD +LADFV  EC K  W+GI+TR+WP TKY+DVIVTG M+
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 317 QYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPN- 376
           QYIPTLDYYS GLPL CTMYASSECYFG+NL P+CKPSEVSYT++PNMAYFEFLP   N 
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 377 --TESDSPPK---------LVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAA 436
             T S S PK         LVDLVDV++G+EYELV+TTYAGLYRYRVGD+L V GF N A
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 438

Query: 437 PQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIPGHYV 496
           PQF F+ RKNVVLSIDSDKTDE ELQ AV+NA   L  + AS+ EYTSYA+T +IPGHYV
Sbjct: 439 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 498

Query: 497 IYWELMVKEAEGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKNGTFEE 556
           ++WEL +       PS  V EDCCL +EESLNSVYRQGRV+D SIG LEI++V++GTF++
Sbjct: 499 LFWELCLNGNTPIPPS--VFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDK 558

Query: 557 LMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTP 593
           LMDYAIS GASINQYK PRCV F PI+ELL+SRVV ++FSP  P+W+P
Sbjct: 559 LMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823335.4e-28079.73Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221901.6e-26376.46Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS04.9e-25774.62Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z64.9e-25774.62Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
P0C0M22.7e-25573.34Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
KAG6576928.10.0100.00putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ... [more]
XP_022922958.10.099.66probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata][more]
XP_023552180.10.099.16probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo... [more]
XP_022985331.10.098.99probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima][more]
XP_038877583.10.095.15probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1E8A00.099.66probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A6J1J7V10.098.99probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1IUJ60.093.79probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1FTR90.093.62probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A5A7T1480.093.16Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
AT2G14960.13.8e-28179.73Auxin-responsive GH3 family protein [more]
AT4G37390.11.8e-27076.78Auxin-responsive GH3 family protein [more]
AT2G23170.11.1e-26476.46Auxin-responsive GH3 family protein [more]
AT1G59500.11.2e-25072.36Auxin-responsive GH3 family protein [more]
AT5G54510.12.1e-23968.71Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 446..466
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 5..594
IPR004993GH3 familyPFAMPF03321GH3coord: 26..567
e-value: 5.1E-207
score: 688.9
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 5..594

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g244530.m01Csor.00g244530.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity