Carg16820 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16820
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionbeta-carotene isomerase D27, chloroplastic-like isoform X1
LocationCarg_Chr19: 92916 .. 96861 (+)
RNA-Seq ExpressionCarg16820
SyntenyCarg16820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGTAGGAGTTGGTGAGGTAAGGAGAGTGATTTTGGGAAGTGGGACAAACGTGAGGAGAAGCAACAAAACGAAGAAGATGAGAAGAAAAGGCATTGCCTGTGTAGCAGTGCTTACAAGAGCAGCAGAGAGTGGAGCAACAGAAGAAACAAGAAAACCAAACACTGTTTATACAGACAACTGGTTTGAGAAAATAGCCATCCACCATGTATCTCAGGCAGTACAAGCAACCTCAGGTGAACTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTGTTTTGAAGATTTGGAAAATAGAATTAATTATGATGTATATTCAGGTTGGAGAAGCAATAAGAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAACTACAACCAGATCAAACAAAGAGAGGTTATCATTCAGGCGCTTGGAAGAGCATTTCCAGCTCCAATCCTCTCTTTGGTAAATTTCATTCATTCATTCATTCATTCAATATCATTTACTATTCTTTTGACTTTCAATGTATTTATGATCTAAATGCATATATCATGCATATATGTAAAATACTATCACCACACATATGGTTAAAGATTTTACCAATACCAATTTCAAGACTACAATCATTTTACTCTCACCACTCCCTCAAATATTATTGCTAAAAGTAATTTCAAAAGTCATATATTTATTTTTCTAGGCAGCTCTAAGTTATTTATTTAGCTATGATATTAGGTGATAAAAGCAATAAATGTTTCCACATCAATAGAAGTTCATTATTAAATAACATTTGTAGGGTAAGTAGTGCATGATCATTTGATGAATGTCATTTAGGCCAAATTTCACTACTTTGTCTTCCTTATTTGGTCTCCCTTCTTCTTCCCATTTGTTTGTTTAAGTATTGACATAATTAAGTTTTTTACCATAACCTATAAGCTAGAAGTTTCAAGTTGATTGTGGTATTAGAGCATGAGGTTATGTGTCCTGTGTTTGAACCCTATAATATAATTTTCTGATTTCGAACTTAAATTCGTAGGATCCAACACATTTTGGGGGATATAGCTAGGTTTCATTGCCTAGGTTAGAACCTACCTGGAACTATACCCTTTTCTAATTTTTCTTTCCATTAATCATGGGGTATAATAGTTATATTTGATTGGCATGTCATTATATGGAAATAGTATCAGCAAATGATCACATTTTATATTATATTTCTAATTATCAGCAATTATTCGATTAAAGAAGTATTAAAACTGTACTTTTACTATTCTCGATCCATTTCCAATTTCGCCTTTTACTATTAAAACCCCCACCCCTCTCAATCGTATGTCCCTAACAAATATAGTGGTGAACAAAGTGCAGATAAGAACATTGCTACCTCAATCCAAATTTGCAAGGGAATATTTTGCTGCATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTAAGATGTTGTAAATAAATTTAGCCAAGGACATAGTTGGGAGTTAACATCAATCCATGGACATAATTGGGAATTAACGGCTGCTTGTATACATACACATTTGGTTAAAGCATATATTATATTATCGACCAAAAAGTCAAAAGTTCGAATTCCTACTCGAAAGAGAGATGGTTCTGTCATAGGGCTTAGGATTGATGTCAAAAAAAAATTTCAGAATATAATAGAGTATTTAAACATTCAACAAACAAGGTCAGAATAGAGATAGATTCCAGCTAATGGTGGACACAAGAACTTCAACTTTAGCACTAAATTCAATATAATAATATGATTTTCAATGGCCAAGTTGATAAACCTGCAGTGTTTGAGGCACTAGTCCCTTCTAGACTTGTCCTGTCCCTACAAATGAAAGAAAGGTTTTGTGACGTAAGATAGATAAACCTATTTTTTAAGAAACTATCTTCTCTTCATGCTTACTCTCTTTGGCCTTTCACTATCTAGGCATTCCTTAAGAACTCAGATTAAACACAATTGATATGTCTAGTTTGTTGGGGAGGGAGTGATTGAAAAAGATTTTTAAGAGAAACGACTAAACAATGCAGGAGCCATTCCCTGCGCGCACACAAATGGAAGAAGCAAGTCTCCTTCTCAATTACTAATTTAGTAACATATGATTCTTACAGGTGAAGGAATCGGACTTCAAAGGAAAAAGAGAAAAAAATGTGGTTCAAATCCACAAGTGCAGGTAATTCTTTCTCGCATCAGAGTTCTATAATTAAGGCAATGTTCTCTAACCGTTCATGGGGTCCCATCTCACCTACTTGAGACAATTTCCACGCCCACAGAACCTTCTGTCTGTAATTATTATGCTTATTCTAAACTCTCAGTACTTAATCACAGTAAGTCATGTCCATCATTCACATCATTGAAAATATGAAATTATCGTTGTCAAAATAACACTCAGCAAATATATATTGTGCCTAAACATATCCCATTTATTTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAGTAAGTCTCCTCTCACTAAGCTATAGAACCACCGAACAATTGCGGCAACCATATACAGTTTTCCCAAAAGAAAAACAAAATTTCAAATTCACATAGACTGCAGCACAAGGCAATTCTAGACACTAGTTCAAGCTGTCTAACTATAACTGAAGGCCATATTTCCTTTTTCTCGTATCCATAGTGTTCAGGTTTGTTTGCACACACCACTACTATTCTTACAAGATAATTGTCTGACTCTATTTAGTTTGGTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCATAGGATATTAAACAATTAAGCAGGTGAGTACAATGGCTAGAGCTCGTTCTCTCTAAGTTTTTTCCTATTTTCATGGGCTCTTTTTTATCACTAAACTAAATCCTGGTGGTTAACTCAAAAGGCCATTATAAAAACTTCATATCCACATATAGAACATTAGAATTAAATTATTTCAAGACCTCTATATGGTTTAGATGAGAGGACACTAAATAAAGTAAATCTGCTATAGCACAATGACTGGTAGATCCATGAGTAAAAGTGAAATTACTATTAAAGCAACATTACCAGCACCAAATGGTGGAAAGGATCATGGTTGTCAATGTGAATGGAGGGTCCCATGCATGAACCATTTTGGGTACAAAAGAACTGCAGATAGGCCCATCCAAGCTAAGTGGGATTTCTAACCTGCAGCTTAATTTTGCAATATGGTTATAATCCAAGAGTTAGAAGCTGAATTCCCATCGTTAATAAATCATCAAGCTGGATTTCCCACTGAGCCAGGTATCGCTCTTGGTCATGATATACATCAATCATCACAGGTGTAAAAAATGCAACTAAGCAAAGGCATTTTTCTTAGGCGTTAATTATGCACCATGATGTAACTATTAGCTGGGAACCACTAGTATTAATGTCAAAGACCAAGAAACACGTCAATAAGCAAGGGGAATCTGGGAGAAAATGGGGGCGGCAAGAAGTATTTAAAAATATGATTAAAATAAGTGTCTTTCTTTGCAGATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCGGCTGCAGTCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGTATTAACCTTACACGAGTCTTCTTCTTCTTTTCTTATTATTACCCTTTACTTCTTTACCTGCAAGTGGAGAAGTCGTATGTTATAATTCACTGGGCAAGGTAGCAGGACTACAGCAGGTCCAAACAAGATGTTTAGGGGGATGGTAATATAAGTTAAAAATGAATTCTAATCTTATTCGTTTACTTTTTGCAGGCAAAACAAAAGAAAAACATACGACAAACTGCTTCATTTAGAGCAAAGGGACGCTTGA

mRNA sequence

ATGGAAGTAGGAGTTGGTGAGGTAAGGAGAGTGATTTTGGGAAGTGGGACAAACGTGAGGAGAAGCAACAAAACGAAGAAGATGAGAAGAAAAGGCATTGCCTGTGTAGCAGTGCTTACAAGAGCAGCAGAGAGTGGAGCAACAGAAGAAACAAGAAAACCAAACACTGTTTATACAGACAACTGGTTTGAGAAAATAGCCATCCACCATGTATCTCAGGCAGTACAAGCAACCTCAGGTTGGAGAAGCAATAAGAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAACTACAACCAGATCAAACAAAGAGAGGTTATCATTCAGGCGCTTGGAAGAGCATTTCCAGCTCCAATCCTCTCTTTGATAAGAACATTGCTACCTCAATCCAAATTTGCAAGGGAATATTTTGCTGCATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTGAAGGAATCGGACTTCAAAGGAAAAAGAGAAAAAAATGTGGTTCAAATCCACAAGTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCGGCTGCAGTCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGTATTAACCTTACACGAGTCTTCTTCTTCTTTTCTTATTATTACCCTTTACTTCTTTACCTGCAAGTGGAGAAGTCGTATGTTATAATTCACTGGGCAAGGCAAAACAAAAGAAAAACATACGACAAACTGCTTCATTTAGAGCAAAGGGACGCTTGA

Coding sequence (CDS)

ATGGAAGTAGGAGTTGGTGAGGTAAGGAGAGTGATTTTGGGAAGTGGGACAAACGTGAGGAGAAGCAACAAAACGAAGAAGATGAGAAGAAAAGGCATTGCCTGTGTAGCAGTGCTTACAAGAGCAGCAGAGAGTGGAGCAACAGAAGAAACAAGAAAACCAAACACTGTTTATACAGACAACTGGTTTGAGAAAATAGCCATCCACCATGTATCTCAGGCAGTACAAGCAACCTCAGGTTGGAGAAGCAATAAGAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAACTACAACCAGATCAAACAAAGAGAGGTTATCATTCAGGCGCTTGGAAGAGCATTTCCAGCTCCAATCCTCTCTTTGATAAGAACATTGCTACCTCAATCCAAATTTGCAAGGGAATATTTTGCTGCATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTGAAGGAATCGGACTTCAAAGGAAAAAGAGAAAAAAATGTGGTTCAAATCCACAAGTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCGGCTGCAGTCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGTATTAACCTTACACGAGTCTTCTTCTTCTTTTCTTATTATTACCCTTTACTTCTTTACCTGCAAGTGGAGAAGTCGTATGTTATAATTCACTGGGCAAGGCAAAACAAAAGAAAAACATACGACAAACTGCTTCATTTAGAGCAAAGGGACGCTTGA

Protein sequence

MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPCYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA
Homology
BLAST of Carg16820 vs. NCBI nr
Match: KAG7011212.1 (Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 599.7 bits (1545), Expect = 1.3e-167
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 297
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 296

BLAST of Carg16820 vs. NCBI nr
Match: XP_022963899.1 (beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 594.3 bits (1531), Expect = 5.6e-166
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGEVRRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 297
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 296

BLAST of Carg16820 vs. NCBI nr
Match: XP_022967513.1 (beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 588.6 bits (1516), Expect = 3.1e-164
Identity = 291/296 (98.31%), Postives = 292/296 (98.65%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGE RRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEERRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRS KSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSKKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGK+EKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKKEKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 297
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 296

BLAST of Carg16820 vs. NCBI nr
Match: KAG6571447.1 (Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564.3 bits (1453), Expect = 6.2e-157
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 279
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 278

BLAST of Carg16820 vs. NCBI nr
Match: XP_022963900.1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 558.9 bits (1439), Expect = 2.6e-155
Identity = 276/278 (99.28%), Postives = 276/278 (99.28%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGEVRRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 279
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 278

BLAST of Carg16820 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.8e-80
Identity = 140/222 (63.06%), Postives = 177/222 (79.73%), Query Frame = 0

Query: 24  KTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRS 83
           K  +++ K    V VL R  +   +EET +   VY DNWF+K+AI+H+S++VQA +G  +
Sbjct: 20  KKLRLKPKHTFVVGVLARPTDD-ISEETLRKTNVYKDNWFDKLAINHLSKSVQAATGISN 79

Query: 84  NKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAA 143
           NKSG++SLVE AT+AS+ +N  +Q+ +I+ AL RAFP PILS+IR ++P SK AREYFA 
Sbjct: 80  NKSGFDSLVEAATVASQKFNTTQQQGIILDALDRAFPKPILSVIRRVMPPSKLAREYFAV 139

Query: 144 FTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKD 203
           FTT+FFAWL+GP EV+ES+  G+REKN+V I KCRFLE+TNC GMCINLCK P Q FIKD
Sbjct: 140 FTTIFFAWLLGPSEVRESEINGRREKNIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKD 199

Query: 204 SLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPCYKLC 246
           S GMPV+MVPNFDDMSCEMIFG++PPA+ DDPALKQPCYKLC
Sbjct: 200 SFGMPVNMVPNFDDMSCEMIFGQEPPASTDDPALKQPCYKLC 240

BLAST of Carg16820 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.0e-67
Identity = 126/234 (53.85%), Postives = 163/234 (69.66%), Query Frame = 0

Query: 28  MRRKGIACVAVLTRAAESGATEETRK------------------PNTVYTDNWFEKIAIH 87
           MRR      AV+ R  E+ A+   +K                  P TVY D+WF+K+AI 
Sbjct: 32  MRRCCSTVRAVMARPQEAPASAPAKKTETAAMMSTVQTETAAAPPATVYRDSWFDKLAIG 91

Query: 88  HVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRT 147
           ++S+ +Q  SG ++ K GYESL++ A   SR ++  KQ E++ QAL RA P+ IL++I+ 
Sbjct: 92  YLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQSEIVTQALERALPSYILTMIKV 151

Query: 148 LLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMC 207
           ++P S+F+REYFAAFTT+FF WLVGPCEV ES+ +G++EKNVV I KCRFLE TNC GMC
Sbjct: 152 MMPPSRFSREYFAAFTTIFFPWLVGPCEVMESEVEGRKEKNVVYIPKCRFLESTNCVGMC 211

Query: 208 INLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPCYK 244
            NLCK PCQ FI+DSLGM V M PNF+DMSCEMIFG+ PP   DDPALKQPC++
Sbjct: 212 TNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCEMIFGQQPPE--DDPALKQPCFR 263

BLAST of Carg16820 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 4.9e-56
Identity = 103/209 (49.28%), Postives = 147/209 (70.33%), Query Frame = 0

Query: 39  LTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSN--KSGYESLVEVAT 98
           L  A E+   E +   N    D++F KIAI+++S+ +Q  +G  S+   + Y+ LV+ AT
Sbjct: 46  LKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTAT 105

Query: 99  MASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPC 158
             SRN++  +Q E ++ +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP 
Sbjct: 106 RVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPS 165

Query: 159 EVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFD 218
           EV+E++  G++EK+VV I KCRFLEQ+NC GMC ++CK P Q FIK+SLGMP+ M P+F+
Sbjct: 166 EVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFN 225

Query: 219 DMSCEMIFGKDPPAAVDDPALKQPCYKLC 246
           D+SC+M+FG++PP   DDPA+KQPC++ C
Sbjct: 226 DLSCKMMFGREPPEIEDDPAMKQPCFEFC 254

BLAST of Carg16820 vs. ExPASy TrEMBL
Match: A0A6J1HLI8 (beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464069 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.7e-166
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGEVRRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 297
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 296

BLAST of Carg16820 vs. ExPASy TrEMBL
Match: A0A6J1HV96 (beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466998 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.5e-164
Identity = 291/296 (98.31%), Postives = 292/296 (98.65%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGE RRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEERRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRS KSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSKKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGK+EKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKKEKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 297
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWARQNKRKTYDKLLHLEQRDA 296

BLAST of Carg16820 vs. ExPASy TrEMBL
Match: A0A6J1HJA1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464069 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.3e-155
Identity = 276/278 (99.28%), Postives = 276/278 (99.28%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGEVRRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 279
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 278

BLAST of Carg16820 vs. ExPASy TrEMBL
Match: A0A6J1HWX8 (beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466998 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 6.9e-154
Identity = 273/278 (98.20%), Postives = 274/278 (98.56%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGE RRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEERRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRS KSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSKKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGK+EKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKKEKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 279
           CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR
Sbjct: 241 CYKLCINLTRVFFFFSYYYPLLLYLQVEKSYVIIHWAR 278

BLAST of Carg16820 vs. ExPASy TrEMBL
Match: A0A6J1HHC5 (beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111464069 PE=4 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.8e-136
Identity = 243/245 (99.18%), Postives = 243/245 (99.18%), Query Frame = 0

Query: 1   MEVGVGEVRRVILGSGTNVRRSNKTKKMRRKGIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEV VGEVRRVILGSGTNVRRSNKTKKMRRK IACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLC 246
           CYKLC
Sbjct: 241 CYKLC 245

BLAST of Carg16820 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 219.5 bits (558), Expect = 3.5e-57
Identity = 103/209 (49.28%), Postives = 147/209 (70.33%), Query Frame = 0

Query: 39  LTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSN--KSGYESLVEVAT 98
           L  A E+   E +   N    D++F KIAI+++S+ +Q  +G  S+   + Y+ LV+ AT
Sbjct: 46  LKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTAT 105

Query: 99  MASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPC 158
             SRN++  +Q E ++ +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP 
Sbjct: 106 RVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPS 165

Query: 159 EVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFD 218
           EV+E++  G++EK+VV I KCRFLEQ+NC GMC ++CK P Q FIK+SLGMP+ M P+F+
Sbjct: 166 EVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFN 225

Query: 219 DMSCEMIFGKDPPAAVDDPALKQPCYKLC 246
           D+SC+M+FG++PP   DDPA+KQPC++ C
Sbjct: 226 DLSCKMMFGREPPEIEDDPAMKQPCFEFC 254

BLAST of Carg16820 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 154.8 bits (390), Expect = 1.1e-37
Identity = 69/154 (44.81%), Postives = 101/154 (65.58%), Query Frame = 0

Query: 88  YESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTV 147
           YES VEV+    +  ++++Q+E + + L    P       R L P +K+A E+ AA T  
Sbjct: 77  YESFVEVSKRVMQGRSRVQQQEAVREVLLSMLPPGAPEQFRKLFPPTKWAAEFNAALTVP 136

Query: 148 FFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGM 207
           FF WLVGP +V E +  G ++++ V+I KCR+LE + C GMC+N+CK P QDF  +  G+
Sbjct: 137 FFHWLVGPSQVIEVEVNGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGL 196

Query: 208 PVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPC 242
           P++M PN++DMSCEMI+G+ PPA  +D A KQPC
Sbjct: 197 PLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPC 230

BLAST of Carg16820 vs. TAIR 10
Match: AT1G03055.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 119.0 bits (297), Expect = 6.4e-27
Identity = 62/142 (43.66%), Postives = 91/142 (64.08%), Query Frame = 0

Query: 39  LTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSN--KSGYESLVEVAT 98
           L  A E+   E +   N    D++F KIAI+++S+ +Q  +G  S+   + Y+ LV+ AT
Sbjct: 46  LKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTAT 105

Query: 99  MASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPC 158
             SRN++  +Q E ++ +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP 
Sbjct: 106 RVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPS 165

Query: 159 EVKESDFKGKREKNVVQIHKCR 179
           EV+E++  G++EK+VV I KCR
Sbjct: 166 EVRETEVNGRKEKSVVYIEKCR 187

BLAST of Carg16820 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 111.7 bits (278), Expect = 1.0e-24
Identity = 69/208 (33.17%), Postives = 108/208 (51.92%), Query Frame = 0

Query: 43  AESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSNKSGYESLVE-VATMASRN 102
           ++ GA +   KP  +  D++F +   + + + V    G  S K GY  L+E V  +  + 
Sbjct: 43  SDEGAPKLEYKPGPL--DDFFMQSFRNKLVEEV----GSDSEKPGYVGLIELVKLLLLKG 102

Query: 103 YNQIKQREVIIQALGRAFPAPILSLIRTL---LPQSKFAREYFAAFTTVFFAWLVGPCEV 162
             + +  +  ++ L   FP  IL L + L   + Q K A    A  T +   WL+GP +V
Sbjct: 103 RTRSETSDAAVRILKSLFPPLILELYKLLIAPIAQGKLAALMVARVTVLTCQWLMGPSKV 162

Query: 163 KESDF-KGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDD 222
              D   G+   + V + KC++LE++ C G+CIN CK P Q F KD +G+P+ M PNF D
Sbjct: 163 NIIDLPNGESWDSGVFVEKCQYLEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKD 222

Query: 223 MSCEMIFGKDPPAAVDDPALKQPCYKLC 246
            SC+  FG  PP   DD  + +PC++ C
Sbjct: 223 YSCQFKFGVAPPE--DDGNVNEPCFETC 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011212.11.3e-167100.00Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_022963899.15.6e-16699.32beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita moschata][more]
XP_022967513.13.1e-16498.31beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita maxima][more]
KAG6571447.16.2e-157100.00Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_022963900.12.6e-15599.28beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
R4HZ962.8e-8063.06Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27... [more]
C7AU218.0e-6753.85Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39... [more]
Q7XA784.9e-5649.28Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D2... [more]
Match NameE-valueIdentityDescription
A0A6J1HLI82.7e-16699.32beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita moschata... [more]
A0A6J1HV961.5e-16498.31beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita maxima O... [more]
A0A6J1HJA11.3e-15599.28beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita moschata... [more]
A0A6J1HWX86.9e-15498.20beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita maxima O... [more]
A0A6J1HHC53.8e-13699.18beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
AT1G03055.13.5e-5749.28unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G64680.11.1e-3744.81unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G03055.26.4e-2743.66unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G01995.11.0e-2433.17unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025114Beta-carotene isomerase D27-like, C-terminalPFAMPF13225DUF4033coord: 151..230
e-value: 5.1E-33
score: 112.9
IPR038938Beta-carotene isomerase D27-likePANTHERPTHR33591BETA-CAROTENE ISOMERASE D27coord: 16..248
NoneNo IPR availablePANTHERPTHR33591:SF1BETA-CAROTENE ISOMERASE D27, CHLOROPLASTICcoord: 16..248

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16820-RACarg16820-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010223 secondary shoot formation
biological_process GO:1901601 strigolactone biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016859 cis-trans isomerase activity
molecular_function GO:0005506 iron ion binding