Carg03752 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03752
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	4-coumarate-CoA ligase
Location	Carg_Chr14: 969482 .. 973101 (-)
RNA-Seq Expression	Carg03752
Synteny	Carg03752

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATCGCGATGGCCAAATCTTTCGATCCCCAATCCCAGATTTACACTTCCCCACGTCCCCCAATCCACTTTCCGACCGACCCCACTATCTCCATTGTTTCGTTTCTCTTCCGAAACTCCTCCTCTTTCCTTCATAGCCTCGCTCTCGCCGACGCTGATTCCGGCGAATCCCTAACTTTCCGTCAGCTTCAAATCCAAGTCTCCAAGTTGGCCCATGCGTTTATCCACCTCGGGATTCGAAAAGGCGATGTGGTTCTCATATTTGCGCCTAATTCCATTCATTTCCCTGTTTGTTTCTTCGCCATTGTTGCCATTGGAGCCATCGCCACCACTTGTAACCCTGCTTACACCTTCGTGGAACTATCAAAGCAAGTCGCGAACTGCAACCCCAAGCTCGTAATCACCGTTCCAGAACTCTGGGACGTAATCGGTAAACTCAACTTGCCTTCTATAATCTTGGGTTCTAAAATTTCTTCGAAATCCTCCCGCCCCAATATCTGGGTTTACTCCGATTTGATCAAAAACTCCGGCGACGTGTCCGATTTGCCTGTGAGTGAAGTGGGTCAGAGCGATGTGGCGGCACTTTTGTATTCATCTGGGACGACAGGGATTAGCAAGGGAGTGATTTTGACTCATAGAAACTTCATAGCGACGTCGTTAATGGTGACCCATGATCAGGACCTTCTTGGTGATCCTAGAAACGTGTTCTTATGCTTTCTCCCAATGTTCCATGTATTTGGTCTCTCTGTAATTCTTTACTCGCAGCTTCAAAGAGGAAACACCGTCGTTTCAATGGCGAAGTTTGAGCTGGAAAAGGCTTTGGGAGTGGTGATGAAGTATAGAATCACTCACCTTTATCTTGTTCCGCCTGTAATTATAGCCATGGCGAAGCAAAGTGTGGTGAAGAAGTATGACTTGTCGTCGTTGAAGCAGATTTTATCTGGGGCGGCGCCTCTTGGGAAGGATGTGATGGAAGAATGTTCTAAGGTTTTACCTCAGACCAAGATCACGCAGGTTTTGAACTGTTCCTTCTCAACTCCTCCATTTTGCACTTTCATTTGGCTGTTACAGGAAATGTTATATGATGTTGTGTTGATTTGGGTAGCAATCTATGGTGTACAGGGGTATGGTATGACAGAAACTTGTGGAGTTATTTCAATGGAGAATTTGGGGGTCGAATCTAGACTATCCGGGTCAACTGGATTCCTTGTTTCAGGAATTGAGGCCCAAATTTTGAGTACAGAAACGCAGAAGCGTCTCCCACCCGGTGAAACAGGGGAGATATGCGTGCGAGGACCAAATATGATGAAAGGTGGATCTCTCTTTGGATATTCTTTAAATGATGAACAGTGCTCATCCATACTAACTATACTGAAGGGTGTATCAAACACCAAACCAGAGTTATACCTTGATCCGATCCCTTCTCTTCTCTAACCGTCAAAACCCATCGCTAGCAAATATTGTCCGTCTTCGACATGATATGTATCGCCACCAGTCTCACGGTTTTTAAAACGCGTATGCTAAGGAGAGGTTTCTACACCTTTATAAAGAATGCTTCATTCTCCCCCCAACATATGTGGGATCTTATGATCCACCCCCTTCCGAGCCCAACATCCTTGTTGGCACATCGCCTCGTGTCCACCCCCTTCAAAGCTCAATCTCTTTGCTGGAGCTCGGTGTCTGACTCCAATACTATTTGTAATATCTCAAGGCCACAGCTACAAATATTGTCTACTTTGGTCTCTTTTTTAAGAGTAAAATTGTGAGATCCTACGTCACCGGAGGACGGGAGGTTACTTTCTTGAACTTGTTTGATTGGATCTGCTAAAAAGAATTGAAATTTCCCCCTGTTTTTAAGATGCCAATCTCGTTTCTCTGATGATTTGTTGTCAGTTTAATCAACTTCGGACCCATTTCAAGCTTTTCCATTTTCATGTCCCAGTCTGATTGGCTAAATGTTTGCATGAACATTTCAGGATATTTCAACAATCAGAAAGCCACCAGCCAAACCATAGACGATCAAGGATGGGTTCATACTGGAGATATCGGATACTTTAATGAAGAAGGAGAATTGTTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTTTCAGGTTCTTTTTTACCTCAACAAGCATCAACTTTTCCATTTGGGAGTGCTTCATTTCCTTTTACTAGGACCGTCCAAACTCATTACTAGCAGATATGTCTTCTTTAGGCTTTTCTTTTAAAACACGTTTGTTAGGGAGAGATTTCCATATCCCTATTTTTAAAGAAGGTTTCATTCTCCTCTTCAACCGATATAGGATCTCACAAATCATTATATCAGAACTTTAACTTCTCTTAAATCAAAAGAAACTGCTATAGATTCTTGTATGCTAAGTTCTTCTCAGTTAAAGAATATGAGTCTAAGATGTACTAATCATATGCTCGGCTCCAGCAAGTATGTCTTCTAATGCTATGTCTGCATTGTCTCCTCTTCTTGGAAGTATCAACTCTAGCTGCATTCGTTGTTGATTGATATCAATTTTTTTGTCACTTTCTTGTCTGCATATTAGGTTGCACCAGCGGAGCTTGAAGCACTGCTACTTTCCCATCCTCAAATAATAGACGCCATTGTGATCCCGTAAGTAGTATATAGTAGCATCTATAGTCCCATCATTATGTGGACGTTCATAGTGGATTTTCCTTTCTATTTCATACTTTCTAGTTAACCTCTGCCATGAATTTCCACCTGCAGATATCCTGATGATAAAGCCGGTGAGGTCCCTATTGCATTTGTTGTTCGCTCACCTAACAGCTCAATAAATGAAGAAGATGTTAAAAGTTTTATCGCTGGTCAGGTAATATTTTGCAAACCCCTCAAACAAGAACAATAAAGAGACTTTTTGTCGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCCCTCTCTAATAAACGCGTTTTAAAAACCTTGAGGAAAAGCCTAAAAGGAAAAGTCTAAGAGGACAATGTCTGTTGGCGATGGCATGGCCCCACCTCACGAATTGGGGTTGCTGTGTCTTTTCCTAAGAGGGGCAGCAGCAAGCCAAGCCTTTACTAAGAGGCCGCCTTTTATGGGCCTGCTGCATAAACCCAAGAAGGCAAAAGATGTGGAAAGATGCAATCTTAAACATTGATTCCCTACGCAAAAAGTGGGAACCATCATTTTGACAAAGGAATTCTTGAAAATGATCAGCTCTTTCCCTTTGCAGGTTGCACCATTCAAGAGATTAAAAAGAGTAACCTTCACGAGTAGCGTTCCGAAGTCGGCTTCGGGAAAGCTTCTGAGAAGGGAGCTCATAGCACAAGTCCGTGCCAAGATGTAGACGTCAAAATGTTGAATAGAAAGAACGTAAGTTATTCTGATACAGTTAGCAAAGAAATGTATTTACAGCTTGATGAACACATGAACAATGCAACACCAATTTACATGGATCATAATAAATAACAGCTTTACTTTTGGGAAAAAGAGAATCATTTCAGAGCCAATAAAAGCAAAAGGAAACAGAAAAGTGTATGATTTAAAATGGAGTGTGAAGATGAGTATGAGATGAAGTAGATGATACCCAACTGTG

mRNA sequence

ATGGCGATCGCGATGGCCAAATCTTTCGATCCCCAATCCCAGATTTACACTTCCCCACGTCCCCCAATCCACTTTCCGACCGACCCCACTATCTCCATTGTTTCGTTTCTCTTCCGAAACTCCTCCTCTTTCCTTCATAGCCTCGCTCTCGCCGACGCTGATTCCGGCGAATCCCTAACTTTCCGTCAGCTTCAAATCCAAGTCTCCAAGTTGGCCCATGCGTTTATCCACCTCGGGATTCGAAAAGGCGATGTGGTTCTCATATTTGCGCCTAATTCCATTCATTTCCCTGTTTGTTTCTTCGCCATTGTTGCCATTGGAGCCATCGCCACCACTTGTAACCCTGCTTACACCTTCGTGGAACTATCAAAGCAAGTCGCGAACTGCAACCCCAAGCTCGTAATCACCGTTCCAGAACTCTGGGACGTAATCGGTAAACTCAACTTGCCTTCTATAATCTTGGGTTCTAAAATTTCTTCGAAATCCTCCCGCCCCAATATCTGGGTTTACTCCGATTTGATCAAAAACTCCGGCGACGTGTCCGATTTGCCTGTGAGTGAAGTGGGTCAGAGCGATGTGGCGGCACTTTTGTATTCATCTGGGACGACAGGGATTAGCAAGGGAGTGATTTTGACTCATAGAAACTTCATAGCGACGTCGTTAATGGTGACCCATGATCAGGACCTTCTTGGTGATCCTAGAAACGTGTTCTTATGCTTTCTCCCAATGTTCCATGTATTTGGTCTCTCTGTAATTCTTTACTCGCAGCTTCAAAGAGGAAACACCGTCGTTTCAATGGCGAAGTTTGAGCTGGAAAAGGCTTTGGGAGTGGTGATGAAGTATAGAATCACTCACCTTTATCTTGTTCCGCCTGTAATTATAGCCATGGCGAAGCAAAGTGTGGTGAAGAAGTATGACTTGTCGTCGTTGAAGCAGATTTTATCTGGGGCGGCGCCTCTTGGGAAGGATGTGATGGAAGAATGTTCTAAGGTTTTACCTCAGACCAAGATCACGCAGGTTTTGAACTGTTCCTTCTCAACTCCTCCATTTTGCACTTTCATTTGGCTGTTACAGGAAATGTTATATGATGTTGTGTTGATTTGGGTAGCAATCTATGGTGTACAGGGGTATGGTATGACAGAAACTTGTGGAGTTATTTCAATGGAGAATTTGGGGGTCGAATCTAGACTATCCGGGTCAACTGGATTCCTTGTTTCAGGAATTGAGGCCCAAATTTTGAGTACAGAAACGCAGAAGCGTCTCCCACCCGGTGAAACAGGGGAGATATGCGTGCGAGGACCAAATATGATGAAAGGATATTTCAACAATCAGAAAGCCACCAGCCAAACCATAGACGATCAAGGATGGGTTCATACTGGAGATATCGGATACTTTAATGAAGAAGGAGAATTGTTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTTTCAGGTTGCACCAGCGGAGCTTGAAGCACTGCTACTTTCCCATCCTCAAATAATAGACGCCATTGTGATCCCATATCCTGATGATAAAGCCGGTGAGGTCCCTATTGCATTTGTTGTTCGCTCACCTAACAGCTCAATAAATGAAGAAGATGTTAAAAGTTTTATCGCTGGTCAGGTTGCACCATTCAAGAGATTAAAAAGAGTAACCTTCACGAGTAGCGTTCCGAAGTCGGCTTCGGGAAAGCTTCTGAGAAGGGAGCTCATAGCACAAGTCCGTGCCAAGATGTAGACGTCAAAATGTTGAATAGAAAGAACGTAAGTTATTCTGATACAGTTAGCAAAGAAATGTATTTACAGCTTGATGAACACATGAACAATGCAACACCAATTTACATGGATCATAATAAATAACAGCTTTACTTTTGGGAAAAAGAGAATCATTTCAGAGCCAATAAAAGCAAAAGGAAACAGAAAAGTGTATGATTTAAAATGGAGTGTGAAGATGAGTATGAGATGAAGTAGATGATACCCAACTGTG

Coding sequence (CDS)

ATGGCGATCGCGATGGCCAAATCTTTCGATCCCCAATCCCAGATTTACACTTCCCCACGTCCCCCAATCCACTTTCCGACCGACCCCACTATCTCCATTGTTTCGTTTCTCTTCCGAAACTCCTCCTCTTTCCTTCATAGCCTCGCTCTCGCCGACGCTGATTCCGGCGAATCCCTAACTTTCCGTCAGCTTCAAATCCAAGTCTCCAAGTTGGCCCATGCGTTTATCCACCTCGGGATTCGAAAAGGCGATGTGGTTCTCATATTTGCGCCTAATTCCATTCATTTCCCTGTTTGTTTCTTCGCCATTGTTGCCATTGGAGCCATCGCCACCACTTGTAACCCTGCTTACACCTTCGTGGAACTATCAAAGCAAGTCGCGAACTGCAACCCCAAGCTCGTAATCACCGTTCCAGAACTCTGGGACGTAATCGGTAAACTCAACTTGCCTTCTATAATCTTGGGTTCTAAAATTTCTTCGAAATCCTCCCGCCCCAATATCTGGGTTTACTCCGATTTGATCAAAAACTCCGGCGACGTGTCCGATTTGCCTGTGAGTGAAGTGGGTCAGAGCGATGTGGCGGCACTTTTGTATTCATCTGGGACGACAGGGATTAGCAAGGGAGTGATTTTGACTCATAGAAACTTCATAGCGACGTCGTTAATGGTGACCCATGATCAGGACCTTCTTGGTGATCCTAGAAACGTGTTCTTATGCTTTCTCCCAATGTTCCATGTATTTGGTCTCTCTGTAATTCTTTACTCGCAGCTTCAAAGAGGAAACACCGTCGTTTCAATGGCGAAGTTTGAGCTGGAAAAGGCTTTGGGAGTGGTGATGAAGTATAGAATCACTCACCTTTATCTTGTTCCGCCTGTAATTATAGCCATGGCGAAGCAAAGTGTGGTGAAGAAGTATGACTTGTCGTCGTTGAAGCAGATTTTATCTGGGGCGGCGCCTCTTGGGAAGGATGTGATGGAAGAATGTTCTAAGGTTTTACCTCAGACCAAGATCACGCAGGTTTTGAACTGTTCCTTCTCAACTCCTCCATTTTGCACTTTCATTTGGCTGTTACAGGAAATGTTATATGATGTTGTGTTGATTTGGGTAGCAATCTATGGTGTACAGGGGTATGGTATGACAGAAACTTGTGGAGTTATTTCAATGGAGAATTTGGGGGTCGAATCTAGACTATCCGGGTCAACTGGATTCCTTGTTTCAGGAATTGAGGCCCAAATTTTGAGTACAGAAACGCAGAAGCGTCTCCCACCCGGTGAAACAGGGGAGATATGCGTGCGAGGACCAAATATGATGAAAGGATATTTCAACAATCAGAAAGCCACCAGCCAAACCATAGACGATCAAGGATGGGTTCATACTGGAGATATCGGATACTTTAATGAAGAAGGAGAATTGTTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTTTCAGGTTGCACCAGCGGAGCTTGAAGCACTGCTACTTTCCCATCCTCAAATAATAGACGCCATTGTGATCCCATATCCTGATGATAAAGCCGGTGAGGTCCCTATTGCATTTGTTGTTCGCTCACCTAACAGCTCAATAAATGAAGAAGATGTTAAAAGTTTTATCGCTGGTCAGGTTGCACCATTCAAGAGATTAAAAAGAGTAACCTTCACGAGTAGCGTTCCGAAGTCGGCTTCGGGAAAGCTTCTGAGAAGGGAGCTCATAGCACAAGTCCGTGCCAAGATGTAG

Protein sequence

MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDVSDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCFLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEMLYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFIAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM

Homology

[Close]

BLAST of Carg03752 vs. NCBI nr
Match: KAG7017376.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
           LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR
Sbjct: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM
Sbjct: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 581

[Close]

BLAST of Carg03752 vs. NCBI nr
Match: KAG6580619.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1039.3 bits (2686), Expect = 1.3e-299
Identity = 542/581 (93.29%), Postives = 544/581 (93.63%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           MAIA+AK FDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT
Sbjct: 1   MAIAVAKYFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT                      
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT---------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                         QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR
Sbjct: 361 --------------QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPF+RLKRVTFTSSVPKSASGKLLRRELIAQVRAKM
Sbjct: 541 IAGQVAPFRRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. NCBI nr
Match: XP_022934454.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 1034.2 bits (2673), Expect = 4.1e-298
Identity = 539/581 (92.77%), Postives = 542/581 (93.29%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSF+FRNSSSFLHSLALADADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGS+ISSKSSRPNIWVYSDLIKNSGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           SD PVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF
Sbjct: 181 SDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT                      
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT---------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                         QGYGMTETCGVISMENLGVESRLSGSTG LVSGIEAQILSTETQKR
Sbjct: 361 --------------QGYGMTETCGVISMENLGVESRLSGSTGLLVSGIEAQILSTETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQ+IDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQVIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRLK VTFTSSVPKSASGKLLRRELIAQVRAKM
Sbjct: 541 IAGQVAPFKRLKIVTFTSSVPKSASGKLLRRELIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. NCBI nr
Match: XP_022982901.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 1028.1 bits (2657), Expect = 3.0e-296
Identity = 533/581 (91.74%), Postives = 542/581 (93.29%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           MAIAMAKSF+PQSQIYTSPRPPIHFPTDPTISI+SFLFRNSSSFLHSLALADADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           E+SKQVA+CNPKLVIT+PELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           SDLPVSEVGQSDVAALLYSSGTTGI+KGVILTHRNFI TSLMVTHDQDLLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVK+YDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT                      
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT---------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                         QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR
Sbjct: 361 --------------QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSP+SSINEEDVKSF
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPSSSINEEDVKSF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRLK VTFTSS+PKSASGKLLRRELIAQVRAKM
Sbjct: 541 IAGQVAPFKRLKTVTFTSSIPKSASGKLLRRELIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. NCBI nr
Match: XP_023526195.1 (4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.5e-295
Identity = 532/581 (91.57%), Postives = 541/581 (93.12%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           +AIAMAKSF+PQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLH+LAL DADSGESLT
Sbjct: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVS+LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANCNPKLVITVPELWDVIGKLNLPS+ILGSKISS+SSRPNIWVYSDLIKNSGDV
Sbjct: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPSVILGSKISSESSRPNIWVYSDLIKNSGDV 182

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFI TSLMVTHDQDLLGDPRNVFLCF
Sbjct: 183 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 242

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 243 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 302

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVK+YDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT                      
Sbjct: 303 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT---------------------- 362

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                         QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR
Sbjct: 363 --------------QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 422

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 423 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 482

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYG+QVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPN+SINEEDVKSF
Sbjct: 483 IKCYGYQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNNSINEEDVKSF 542

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRLK VTFTSSVPKSASGKLLRRELIAQVRAKM
Sbjct: 543 IAGQVAPFKRLKIVTFTSSVPKSASGKLLRRELIAQVRAKM 547

[Close]

BLAST of Carg03752 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 9.2e-208
Identity = 370/579 (63.90%), Postives = 455/579 (78.58%), Query Frame = 0


Query: 5   MAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQL 64
           M K F+P++QIY+S RPP++FPTDP +S+ SFLFR+S+S+ +  AL DADSG++LTF +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 65  QIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSK 124
           + QVSKLAH+ + L I+K DVVLIFAPNSIHFPVCFF+I A+GAI TTCNP+YTF ELS 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 125 QVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSK-SSRPNIWVYSDLIKNSGDVSDL 184
           Q  +CNP LVITVPELW+   KLNLP+IIL S  +SK SS+   W +SDL + S   S+L
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRFSEL 180

Query: 185 PVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCFLPM 244
           P+S+V QSDVAALLYSSGTTG SKGV+L+H+NFI TSLMVT DQD  GDP+N+ +CFLPM
Sbjct: 181 PISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLPM 240

Query: 245 FHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS-VV 304
           FH+FGLSVI YSQL+RGN VVSM KFELE AL  V  YR+THL++VPPV+IA+AK+S VV
Sbjct: 241 FHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVVV 300

Query: 305 KKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEMLY 364
           ++YDLSS+K+ILSGAAPLGK+VME+C++ +P   I                         
Sbjct: 301 RRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAI------------------------- 360

Query: 365 DVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRLP 424
                      +QGYGMTETCG+IS+E+     R SGSTG L  GIE+QI+ T+    LP
Sbjct: 361 -----------IQGYGMTETCGIISIEDSKKGCRFSGSTGMLAPGIESQIMDTKRWNPLP 420

Query: 425 PGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIK 484
           P + GEI +RGPNMM+GY NN +AT  TID+QGWV TGDIGYF+EEG+LFVVDR+KELIK
Sbjct: 421 PNQEGEIWLRGPNMMQGYLNNLEATKSTIDEQGWVKTGDIGYFDEEGQLFVVDRLKELIK 480

Query: 485 CYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFIA 544
           CYGFQVAPAELEALLLSHP+I+DA+VIP+PD+KAGEVPIA VVRSPNSS++EEDV+ FI 
Sbjct: 481 CYGFQVAPAELEALLLSHPEILDAVVIPFPDEKAGEVPIANVVRSPNSSLSEEDVQRFIE 540

Query: 545 GQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
            QVAPFK+L+RVTF SSV KS +GK+LRRELI +VR+K+
Sbjct: 541 KQVAPFKKLRRVTFVSSVLKSPAGKILRRELIQKVRSKI 543

[Close]

BLAST of Carg03752 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 7.8e-191
Identity = 343/580 (59.14%), Postives = 433/580 (74.66%), Query Frame = 0


Query: 5   MAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQL 64
           M KS   +  ++ S RPP+  P D  +S+VSF+FRNSSS+    AL D+D+ E+L+F Q 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 65  QIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSK 124
           +  V K++H F++LG++K DVVLIFAPNSIH PVCF  IVA GAIATT NP YT  ELSK
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 125 QVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKS---SRPNIWVYSDLIKNSGDVS 184
           QV + NPKL++TVPEL++ +   NLP+I++G      S   SR  +  + DL+  SG VS
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGPVS 180

Query: 185 DLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCFL 244
           D P+ +  QSD AALLYSSGTTG+SKGV+L+H+NFIA+SLMVT +QD  G+  NVFLCFL
Sbjct: 181 DFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCFL 240

Query: 245 PMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV 304
           PMFHVFGL++I Y+QLQRGNTV+SMA+F+LEK L  V KY++THL++VPPVI+A+ K S+
Sbjct: 241 PMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNSI 300

Query: 305 VKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEML 364
           VKKYDLSSLK I SGAAPLGKD+MEEC+K++P   +                        
Sbjct: 301 VKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIV------------------------ 360

Query: 365 YDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRL 424
                        QGYGMTETCG++S+E+     R +GS G L SG+EAQI+S +T K L
Sbjct: 361 ------------AQGYGMTETCGIVSVEDTRGGKRHTGSAGMLSSGVEAQIVSVDTLKPL 420

Query: 425 PPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELI 484
           PP + GEI VRGPNMM+GYFNN +AT  TID +GWVHTGD+GYF+E+G L+VVDRIKELI
Sbjct: 421 PPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKGWVHTGDLGYFDEDGHLYVVDRIKELI 480

Query: 485 KCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFI 544
           K  GFQVAPAELE LL+SHP+I+DA+VIP+PD  AGEVP+A+VVRSPNSS+ E+DVK FI
Sbjct: 481 KYKGFQVAPAELEGLLVSHPEILDAVVIPFPDADAGEVPVAYVVRSPNSSLTEDDVKKFI 540

Query: 545 AGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           AGQVA FKRL++VTF +SVPKSASGK+LRRELI +VR+ +
Sbjct: 541 AGQVASFKRLRKVTFINSVPKSASGKILRRELIQKVRSNI 544

[Close]

BLAST of Carg03752 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 5.2e-187
Identity = 343/580 (59.14%), Postives = 427/580 (73.62%), Query Frame = 0


Query: 5   MAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQL 64
           M KS   +  IY S RP +  P DP  S+VSFLFRNSSS+   LA+AD+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  QIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSK 124
           +  V++LAH F  LGIRK DVVLIFAPNS  FP+CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSK---SSRPNIWVYSDLIKNSGDVS 184
           Q+ + NPK++I+V +L+D I   +LP ++LGSK + +    S   I  + ++++ S  VS
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 185 DLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCFL 244
           + P  E+ QSD AALLYSSGTTG SKGV LTH NFIA SLMVT DQDL+G+   VFLCFL
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 245 PMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV 304
           PMFHVFGL+VI YSQLQRGN +VSMA+FELE  L  + K+R+THL++VPPV +A++KQS+
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 305 VKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEML 364
           VKK+DLSSLK I SGAAPLGKD+MEEC + +P   +                        
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLL------------------------ 360

Query: 365 YDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRL 424
                       +QGYGMTETCG++S+E+  +  R SGS G L  G+EAQI+S ET K  
Sbjct: 361 ------------MQGYGMTETCGIVSVEDPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQ 420

Query: 425 PPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELI 484
           PP + GEI VRGPNMMKGY NN +AT +TID + WVHTGD+GYFNE+G L+VVDRIKELI
Sbjct: 421 PPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIKELI 480

Query: 485 KCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFI 544
           K  GFQVAPAELE LL+SHP I+DA+VIP+PD++AGEVPIAFVVRSPNSSI E+D++ FI
Sbjct: 481 KYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFI 540

Query: 545 AGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           A QVAP+KRL+RV+F S VPKSA+GK+LRREL+ QVR+KM
Sbjct: 541 AKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSKM 544

[Close]

BLAST of Carg03752 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 552.0 bits (1421), Expect = 8.2e-156
Identity = 307/573 (53.58%), Postives = 394/573 (68.76%), Query Frame = 0


Query: 15  IYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADS-GESLTFRQLQIQVSKLAH 74
           +Y S RPP    +DP +S+   L R + +   ++ALADA + G +LTF +L+  V   A 
Sbjct: 16  VYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRSAVLSTAV 75

Query: 75  AF-IHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSKQVANCNPK 134
           A     G+R GD VL+ APN + +PVCFFA+ A+GA+ TT NP YT  E++KQV++   K
Sbjct: 76  ALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQVSDARAK 135

Query: 135 LVITVPELWDVIGKLNLPSIILGSKISSKSSR----PNIWVYSDLIKNSGDVSDLPVSEV 194
           LVIT+  L   I  L LP I+L    ++ ++       + +Y++L+    + +D     +
Sbjct: 136 LVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKE-ADYRRPPI 195

Query: 195 GQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCFLPMFHVFG 254
            QSD AALLYSSGTTG SKGVILTHRNFIA + MVT DQD   +  NVFLCFLPMFH+FG
Sbjct: 196 KQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLPMFHIFG 255

Query: 255 LSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVVKKYDLS 314
           LSVI Y+QL RGN +++M++F++   +  V ++R+THL+ VPPVIIA+AK     KYDLS
Sbjct: 256 LSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKAGKYDLS 315

Query: 315 SLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEMLYDVVLIW 374
           SLK I SGAAPLGKDVME  +K  P ++I                               
Sbjct: 316 SLKFIGSGAAPLGKDVMEVVAKKFPDSEI------------------------------- 375

Query: 375 VAIYGVQGYGMTETCGVISME--NLGVESRLSGSTGFLVSGIEAQILSTETQKRLPPGET 434
                VQGYGMTETCG+IS+E    G ++R  GSTG LVSG+EA+I+  +T K LPP + 
Sbjct: 376 -----VQGYGMTETCGIISLEYPEKG-QAREFGSTGTLVSGVEAKIVDIKTLKHLPPNQV 435

Query: 435 GEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIKCYGF 494
           GEICVRGPN+M+GYFNN +AT  TI  QGW+HTGD+GYF+  G+LFVVDR+KELIK  GF
Sbjct: 436 GEICVRGPNVMQGYFNNVQATEFTI-KQGWLHTGDLGYFDGGGQLFVVDRLKELIKYKGF 495

Query: 495 QVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFIAGQVA 554
           Q+APAELE LLLSHP+I+DA+VIP+PD KAGEVPIA+VVRSP+SS+ E DV+ FI  QVA
Sbjct: 496 QIAPAELEGLLLSHPEILDAVVIPFPDAKAGEVPIAYVVRSPDSSLTEVDVQKFIEKQVA 549

Query: 555 PFKRLKRVTFTSSVPKSASGKLLRRELIAQVRA 580
            +KRLKRVTF  SVPKSASGK+LRR+LIAQVR+
Sbjct: 556 YYKRLKRVTFVGSVPKSASGKILRRQLIAQVRS 549

[Close]

BLAST of Carg03752 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.5e-117
Identity = 239/579 (41.28%), Postives = 353/579 (60.97%), Query Frame = 0


Query: 13  SQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQLQIQVSKLA 72
           + I+ S R P+  P + ++ + +F+  +S +    +A  DA +G  LTF QL   V  +A
Sbjct: 19  NSIFYSKREPVQLPQNHSVDVTTFI--SSRAHHGKIAFIDAATGRHLTFPQLWRAVDSVA 78

Query: 73  HAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSKQVANCNPK 132
                +GIRKGDV+L+ +PNSI+FPV   A++++GAI TT NP  T  E++KQ+ +  P 
Sbjct: 79  TCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITDSKPV 138

Query: 133 LVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDVSDLPVS------ 192
           L  T+P+L   I   NLP +I+  ++ S   +       +++ + G++     S      
Sbjct: 139 LAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKT-----LNIVSSLGEMMRKEPSPNRIGY 198

Query: 193 EVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLG--DPRNVFLCFLPMF 252
            V Q D A LLYSSGTTG SKGV+ +H+N IA   MV       G  D  + F+C +PMF
Sbjct: 199 RVNQEDTATLLYSSGTTGASKGVVSSHKNLIA---MVQTILSRFGTEDGEHTFICTVPMF 258

Query: 253 HVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS--VV 312
           H++GL+      L  G+T+V ++KFE+ + L  + KYR T+L LVPP+++A+ K +  + 
Sbjct: 259 HIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIR 318

Query: 313 KKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEMLY 372
            KYDLSSL+ +LSG APL K+V+E   +  P   I                         
Sbjct: 319 AKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSI------------------------- 378

Query: 373 DVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRLP 432
                      +QGYG+TE+ G+ +  +   ESR  G+ G L   +EA+I++ ET + L 
Sbjct: 379 -----------LQGYGLTESTGIGASTDCLQESRRYGTAGMLSPSMEAKIVNPETGEALS 438

Query: 433 PGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIK 492
              TGE+ +RGP +MKGYF+N++ATS TID +GW+ TGD+ Y +E+G +FVVDR+KELIK
Sbjct: 439 VNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIK 498

Query: 493 CYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFIA 552
             G+QVAPAELEALLLSHP+I DA VIPYPD +AG+ P+A+VVR   S+++E  V  FIA
Sbjct: 499 YKGYQVAPAELEALLLSHPEISDAAVIPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIA 551

Query: 553 GQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
             VAP+KR+++V F +S+PK+ SGK+LR++LI    +K+
Sbjct: 559 KLVAPYKRIRKVAFVASIPKNPSGKILRKDLIKLATSKL 551

[Close]

BLAST of Carg03752 vs. ExPASy TrEMBL
Match: A0A6J1F2M7 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=4 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 2.0e-298
Identity = 539/581 (92.77%), Postives = 542/581 (93.29%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSF+FRNSSSFLHSLALADADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGS+ISSKSSRPNIWVYSDLIKNSGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           SD PVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF
Sbjct: 181 SDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT                      
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT---------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                         QGYGMTETCGVISMENLGVESRLSGSTG LVSGIEAQILSTETQKR
Sbjct: 361 --------------QGYGMTETCGVISMENLGVESRLSGSTGLLVSGIEAQILSTETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQ+IDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQVIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRLK VTFTSSVPKSASGKLLRRELIAQVRAKM
Sbjct: 541 IAGQVAPFKRLKIVTFTSSVPKSASGKLLRRELIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. ExPASy TrEMBL
Match: A0A6J1J0P0 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 1.4e-296
Identity = 533/581 (91.74%), Postives = 542/581 (93.29%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           MAIAMAKSF+PQSQIYTSPRPPIHFPTDPTISI+SFLFRNSSSFLHSLALADADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           E+SKQVA+CNPKLVIT+PELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           SDLPVSEVGQSDVAALLYSSGTTGI+KGVILTHRNFI TSLMVTHDQDLLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVK+YDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT                      
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKIT---------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                         QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR
Sbjct: 361 --------------QGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSP+SSINEEDVKSF
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPSSSINEEDVKSF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRLK VTFTSS+PKSASGKLLRRELIAQVRAKM
Sbjct: 541 IAGQVAPFKRLKTVTFTSSIPKSASGKLLRRELIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. ExPASy TrEMBL
Match: A0A5A7TKZ4 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002730 PE=4 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 5.1e-270
Identity = 487/581 (83.82%), Postives = 517/581 (88.98%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           M I M KSF+PQS+IY+SPRPPIHFPTDP ISIVSFLFRNSSS+ ++LALADADSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLA  F  LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANCNPKLVITVPELWDVIGKLNLPS+ILGSKISSK SR NIW YSDLIK +GDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGISKGVILTHRNFI TSLMVT DQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVI+YSQLQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ 
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVK YDLSSL+QILSGAAPLGKDVMEECSK++PQ +I                       
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARI----------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                        +QGYGMTETCGVIS+EN+ VES LSG+TG L SGIEAQILS ETQKR
Sbjct: 361 -------------IQGYGMTETCGVISVENMVVESTLSGATGSLGSGIEAQILSIETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQI DAIVIP+PDDKAGEVPIAFVVRSPNSSI+EEDVK F
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGEVPIAFVVRSPNSSISEEDVKKF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRL++VTFTSSVPKSASGKLLRRE+IAQVRAKM
Sbjct: 541 IAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. ExPASy TrEMBL
Match: A0A1S3B5U8 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 5.1e-270
Identity = 487/581 (83.82%), Postives = 517/581 (88.98%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           M I M KSF+PQS+IY+SPRPPIHFPTDP ISIVSFLFRNSSS+ ++LALADADSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLA  F  LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANCNPKLVITVPELWDVIGKLNLPS+ILGSKISSK SR NIW YSDLIK +GDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGISKGVILTHRNFI TSLMVT DQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLSVI+YSQLQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ 
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVK YDLSSL+QILSGAAPLGKDVMEECSK++PQ +I                       
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARI----------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                        +QGYGMTETCGVIS+EN+ VES LSG+TG L SGIEAQILS ETQKR
Sbjct: 361 -------------IQGYGMTETCGVISVENMVVESTLSGATGSLGSGIEAQILSIETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQI DAIVIP+PDDKAGEVPIAFVVRSPNSSI+EEDVK F
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGEVPIAFVVRSPNSSISEEDVKKF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           IAGQVAPFKRL++VTFTSSVPKSASGKLLRRE+IAQVRAKM
Sbjct: 541 IAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. ExPASy TrEMBL
Match: A0A0A0LDK0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.7e-268
Identity = 481/581 (82.79%), Postives = 515/581 (88.64%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           M I M KSF+PQSQ+YTS RPPIHFPTDPTISIVSFLFRNSSS+ ++LAL DADSGESLT
Sbjct: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           FRQLQIQVSKLAH FI LGI+KGDVVLIF+PNSIHF VCFFAIVAIGAIATTCNPAYT  
Sbjct: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFSPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120

Query: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180
           ELSKQVANC PKLVITVPELWDVIGKLNLPSIILGSKISSK SR NIW YSDLIK +GDV
Sbjct: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWGYSDLIKKAGDV 180

Query: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGISKGVILTHRNFI  SLMVT DQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300
           LPMFHVFGLS+++ SQLQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ 
Sbjct: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360
           VVK YDLSSL+QILSGAAPLGKDVM+ECSK++PQ +I                       
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARI----------------------- 360

Query: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420
                        +QGYGMTETCGVIS+EN+GVES  SG+TG LVSG+EAQILS ETQKR
Sbjct: 361 -------------IQGYGMTETCGVISVENVGVESTQSGATGSLVSGVEAQILSIETQKR 420

Query: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480
           LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL
Sbjct: 421 LPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKEL 480

Query: 481 IKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSF 540
           IKCYGFQVAPAELEALLLSHPQI DAIVIP+PDDKAGEVPIAFVVRSPNSSI+EEDVK F
Sbjct: 481 IKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKAGEVPIAFVVRSPNSSISEEDVKIF 540

Query: 541 IAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           +AGQVAPFKRL++VTFTSSVPKSASGKLLRRE+IAQVRAKM
Sbjct: 541 VAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQVRAKM 545

[Close]

BLAST of Carg03752 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 655.6 bits (1690), Expect = 3.7e-188
Identity = 343/580 (59.14%), Postives = 427/580 (73.62%), Query Frame = 0


Query: 5   MAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQL 64
           M KS   +  IY S RP +  P DP  S+VSFLFRNSSS+   LA+AD+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  QIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSK 124
           +  V++LAH F  LGIRK DVVLIFAPNS  FP+CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSK---SSRPNIWVYSDLIKNSGDVS 184
           Q+ + NPK++I+V +L+D I   +LP ++LGSK + +    S   I  + ++++ S  VS
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 185 DLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCFL 244
           + P  E+ QSD AALLYSSGTTG SKGV LTH NFIA SLMVT DQDL+G+   VFLCFL
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 245 PMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV 304
           PMFHVFGL+VI YSQLQRGN +VSMA+FELE  L  + K+R+THL++VPPV +A++KQS+
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 305 VKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEML 364
           VKK+DLSSLK I SGAAPLGKD+MEEC + +P   +                        
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLL------------------------ 360

Query: 365 YDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRL 424
                       +QGYGMTETCG++S+E+  +  R SGS G L  G+EAQI+S ET K  
Sbjct: 361 ------------MQGYGMTETCGIVSVEDPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQ 420

Query: 425 PPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELI 484
           PP + GEI VRGPNMMKGY NN +AT +TID + WVHTGD+GYFNE+G L+VVDRIKELI
Sbjct: 421 PPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIKELI 480

Query: 485 KCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFI 544
           K  GFQVAPAELE LL+SHP I+DA+VIP+PD++AGEVPIAFVVRSPNSSI E+D++ FI
Sbjct: 481 KYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFI 540

Query: 545 AGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           A QVAP+KRL+RV+F S VPKSA+GK+LRREL+ QVR+KM
Sbjct: 541 AKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSKM 544

[Close]

BLAST of Carg03752 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 403.7 bits (1036), Expect = 2.6e-112
Identity = 232/582 (39.86%), Postives = 345/582 (59.28%), Query Frame = 0


Query: 9   FDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQLQIQV 68
           F   +  + S R PI  P +P++ + +F+  +S +    +A  DA +G++LTF +L   V
Sbjct: 10  FCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELWRAV 69

Query: 69  SKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSKQVAN 128
             +A     +GIRKG VVL+ +PNSI FPV   +++++GAI TT NP  T  E++KQ+ +
Sbjct: 70  ESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKD 129

Query: 129 CNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDVSDL----- 188
            NP L  T  +L        LP I   +K       P + +  + + + GDV  L     
Sbjct: 130 SNPVLAFTTSQL--------LPKISAAAK-----KLPIVLMDEERVDSVGDVRRLVEMMK 189

Query: 189 -------PVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLG--DPR 248
                      V Q D A LLYSSGTTG+SKGVI +HRN IA   MV    +  G  D  
Sbjct: 190 KEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIA---MVQTIVNRFGSDDGE 249

Query: 249 NVFLCFLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVII 308
             F+C +PMFH++GL+      L  G+T++ ++KFE+ + +  + KY+ T L LVPP+++
Sbjct: 250 QRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILV 309

Query: 309 AMAK--QSVVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCT 368
           AM      +  KYDLSS+  +L G APL K+V E  ++  P  KI               
Sbjct: 310 AMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKI--------------- 369

Query: 369 FIWLLQEMLYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQI 428
                                +QGYG+TE+ G+ +  +   ESR  G+ G L + +E +I
Sbjct: 370 ---------------------LQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRI 429

Query: 429 LSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELF 488
           +   T + L P +TGE+ ++GP++MKGYF+N++ATS T+D +GW+ TGD+ Y +E+G +F
Sbjct: 430 VDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIF 489

Query: 489 VVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSI 548
           VVDR+KELIK  G+QVAPAELEALLL+HP+I DA VIP+PD + G+ P+A+VVR   SS+
Sbjct: 490 VVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSL 537

Query: 549 NEEDVKSFIAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELI 575
           +E+ +  F+A QVAP+KR+++V F SS+PK+ SGK+LR++LI
Sbjct: 550 SEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 537

[Close]

BLAST of Carg03752 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.3e-108
Identity = 225/571 (39.40%), Postives = 339/571 (59.37%), Query Frame = 0


Query: 9   FDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQLQIQV 68
           F  ++ IYTS  P +H P DP +  VS LF  S       AL D+ +G S++  +LQI V
Sbjct: 25  FSSKTGIYTSKFPSLHLPVDPNLDAVSALF--SHKHHGDTALIDSLTGFSISHTELQIMV 84

Query: 69  SKLAHAFIH-LGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSKQVA 128
             +A    H LG+R+GDVV +  PNS++FP+ F +++++GAI TT NP+ +  E+ KQV+
Sbjct: 85  QSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVS 144

Query: 129 NCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSR-PNIWVYSDLIKNSGDVSDLPVS 188
            C+  L  T  E  + +  L +  I +       S R  N   YS + ++ G V   P  
Sbjct: 145 ECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESFGFV---PKP 204

Query: 189 EVGQSDVAALLYSSGTTGISKGVILTHRNFIAT-SLMVTHDQDLLGDP--RNVFLCFLPM 248
            + Q DVAA++YSSGTTG SKGV+LTHRN IA+  L V  +      P   NV+L  LP+
Sbjct: 205 LIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPL 264

Query: 249 FHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV-V 308
            H++GLS+ +   L  G+T+V M +F+    + V+ +++ITH  +VPP+++A+ K++  V
Sbjct: 265 CHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGV 324

Query: 309 KKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEMLY 368
                 SLKQ+ SGAAPL +  +E+  + LP   +                         
Sbjct: 325 CGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDL------------------------- 384

Query: 369 DVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRLP 428
                      +QGYGMTE+  V +      +     S G L   ++A+++   +   LP
Sbjct: 385 -----------IQGYGMTESTAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLP 444

Query: 429 PGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIK 488
           PG  GE+ ++GP +MKGY NN KAT  +I +  W+ TGDI YF+E+G LF+VDRIKE+IK
Sbjct: 445 PGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIK 504

Query: 489 CYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFIA 548
             GFQ+APA+LEA+L+SHP IIDA V   P+++ GE+P+AFVVR   ++++EEDV S++A
Sbjct: 505 YKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVA 554

Query: 549 GQVAPFKRLKRVTFTSSVPKSASGKLLRREL 574
            QVAP++++++V   +S+PKS +GK+LR+EL
Sbjct: 565 SQVAPYRKVRKVVMVNSIPKSPTGKILRKEL 554

[Close]

BLAST of Carg03752 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 382.9 bits (982), Expect = 4.7e-106
Identity = 232/574 (40.42%), Postives = 333/574 (58.01%), Query Frame = 0


Query: 11  PQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLTFRQLQIQVSK 70
           P  +I+ S  P I  P    + + ++ F   SS      L    +G+S T+ +  +   +
Sbjct: 30  PTPRIFRSKLPDIDIPNH--LPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRR 89

Query: 71  LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFVELSKQVANCN 130
           +A     LGIRKGDV++I   NS  F   F     IGA++TT NP YT  EL KQ+ +  
Sbjct: 90  VASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSG 149

Query: 131 PKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDVSDLPVSEVGQ 190
            KL+IT  +  D +  L     ++    + + +  N   +S LI +          ++G 
Sbjct: 150 AKLIITHSQYVDKLKNLGENLTLI---TTDEPTPENCLPFSTLITDDETNPFQETVDIGG 209

Query: 191 SDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQD---LLGDPRNVFLCFLPMFHVF 250
            D AAL +SSGTTG+ KGV+LTH++ I TS+    D D   L     +V LC LP+FH++
Sbjct: 210 DDAAALPFSSGTTGLPKGVVLTHKSLI-TSVAQQVDGDNPNLYLKSNDVILCVLPLFHIY 269

Query: 251 GLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVVKKYDL 310
            L+ +L + L+ G TV+ M KFE+   L ++ ++R+T   LVPP++IA+AK   V  YDL
Sbjct: 270 SLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDL 329

Query: 311 SSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEMLYDVVLI 370
           SS++ +LSGAAPLGK++ +   + LPQ                                 
Sbjct: 330 SSVRFVLSGAAPLGKELQDSLRRRLPQ--------------------------------- 389

Query: 371 WVAIYGVQGYGMTETCGVISMENLGVESR----LSGSTGFLVSGIEAQILSTETQKRLPP 430
             AI G QGYGMTE   V+SM +LG         SGS G +V   E +++  ET+  L  
Sbjct: 390 --AILG-QGYGMTEAGPVLSM-SLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGY 449

Query: 431 GETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIKC 490
            + GEIC+RG  +MK Y N+ +ATS TID++GW+HTGDIGY +E+ E+F+VDR+KE+IK 
Sbjct: 450 NQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKF 509

Query: 491 YGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVRSPNSSINEEDVKSFIAG 550
            GFQV PAELE+LL++H  I DA V+P  D+ AGEVP+AFVVRS  + I EEDVK ++A 
Sbjct: 510 KGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAK 560

Query: 551 QVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQV 578
           QV  +KRL +V F +S+PKS SGK+LR++L A++
Sbjct: 570 QVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of Carg03752 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 381.7 bits (979), Expect = 1.0e-105
Identity = 223/596 (37.42%), Postives = 343/596 (57.55%), Query Frame = 0


Query: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60
           +A+     F   + I+ S R P+  P +  + + SF+   S          DA +G  L+
Sbjct: 19  LAVDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFI--ASQPHRGKTVFVDAVTGRRLS 78

Query: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120
           F +L + V ++A     LG+RKG+VV+I +PNSI FP+   +++++GAI TT NP  T  
Sbjct: 79  FPELWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSD 138

Query: 121 ELSKQVANCNPKLVITVPELWDVI---GKLNLPSIILGS-KISSKSSRPNIWVYSDLIKN 180
           E+SKQ+ +  P L  T  +L   +      NLP +++    + S+S       Y D +K 
Sbjct: 139 EISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQS-------YGDRVKL 198

Query: 181 SGDVSDLPVSE---------VGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQ 240
            G +  +  +E         V Q D AALLYSSGTTG SKGV+L+HRN IA   +V   +
Sbjct: 199 VGRLETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA---LVQAYR 258

Query: 241 DLLGDPRNVFLCFLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLY 300
              G  +   +C +PM H+FG        +  G T+V + KF++ K L  V  +R ++L 
Sbjct: 259 ARFGLEQRT-ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLS 318

Query: 301 LVPPVIIAMAK--QSVVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSF 360
           LVPP+++AM      +  KYDLSSL  +++G APL ++V E+  +  P+ KI        
Sbjct: 319 LVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKI-------- 378

Query: 361 STPPFCTFIWLLQEMLYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLV 420
                                       +QGYG+TE+  + +      E++  G++G L 
Sbjct: 379 ----------------------------LQGYGLTESTAIAASMFNKEETKRYGASGLLA 438

Query: 421 SGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYF 480
             +E +I+  +T + L   +TGE+ +R P +MKGYF N++AT+ TID +GW+ TGD+ Y 
Sbjct: 439 PNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTIDSEGWLKTGDLCYI 498

Query: 481 NEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVV 540
           + +G +FVVDR+KELIKC G+QVAPAELEALLL+HP+I DA VIP PD KAG+ P+A++V
Sbjct: 499 DGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIPDMKAGQYPMAYIV 558

Query: 541 RSPNSSINEEDVKSFIAGQVAPFKRLKRVTFTSSVPKSASGKLLRRELIAQVRAKM 582
           R   S+++E ++  F+A QV+P+K++++VTF +S+PK+ SGK+LRREL     +K+
Sbjct: 559 RKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRELTKLTTSKL 565

The following BLAST results are available for this feature:

BLAST of Carg03752 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017376.1	0.0e+00	100.00	4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
KAG6580619.1	1.3e-299	93.29	4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022934454.1	4.1e-298	92.77	4-coumarate--CoA ligase-like 7 [Cucurbita moschata]	[more]
XP_022982901.1	3.0e-296	91.74	4-coumarate--CoA ligase-like 7 [Cucurbita maxima]	[more]
XP_023526195.1	2.5e-295	91.57	4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg03752 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQS1	9.2e-208	63.90	Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1	[more]
M4IRL6	7.8e-191	59.14	Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1	[more]
Q9M0X9	5.2e-187	59.14	4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=...	[more]
Q0DV32	8.2e-156	53.58	4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1...	[more]
M4IQR7	1.5e-117	41.28	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]

BLAST of Carg03752 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2M7	2.0e-298	92.77	4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=...	[more]
A0A6J1J0P0	1.4e-296	91.74	4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 ...	[more]
A0A5A7TKZ4	5.1e-270	83.82	4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B5U8	5.1e-270	83.82	4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1	[more]
A0A0A0LDK0	3.7e-268	82.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1	[more]

BLAST of Carg03752 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05160.1	3.7e-188	59.14	AMP-dependent synthetase and ligase family protein	[more]
AT1G20510.1	2.6e-112	39.86	OPC-8:0 CoA ligase1	[more]
AT4G19010.1	1.3e-108	39.40	AMP-dependent synthetase and ligase family protein	[more]
AT1G65060.1	4.7e-106	40.42	4-coumarate:CoA ligase 3	[more]
AT1G20480.1	1.0e-105	37.42	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.300.30		coord: 479..580 e-value: 8.2E-29 score: 102.3
None	No IPR available	PANTHER	PTHR24096:SF373	4-COUMARATE:COA LIGASE-LIKE PROTEIN	coord: 8..580
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 8..580
None	No IPR available	CDD	cd05904	4CL	coord: 25..573 e-value: 0.0 score: 723.64
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 16..579
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 492..567 e-value: 2.3E-14 score: 54.1
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 374..483 e-value: 3.8E-38 score: 131.2 coord: 48..339 e-value: 2.5E-59 score: 201.0
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 14..478 e-value: 8.6E-124 score: 415.5
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 196..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03752-RA	Carg03752-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016405	CoA-ligase activity