Carg19894-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg19894-RA vs. NCBI nr
Match: KAG6583944.1 (hypothetical protein SDJN03_19876, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019561.1 hypothetical protein SDJN02_18522, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 460.7 bits (1184), Expect = 7.6e-126
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI
Sbjct: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG 120
           SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG
Sbjct: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG 120

Query: 121 GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180
           GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG
Sbjct: 121 GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180

Query: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 235
           GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR
Sbjct: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 234

BLAST of Carg19894-RA vs. NCBI nr
Match: XP_022927119.1 (uncharacterized protein LOC111434058 [Cucurbita moschata])

HSP 1 Score: 452.6 bits (1163), Expect = 2.1e-123
Identity = 229/234 (97.86%), Postives = 232/234 (99.15%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MDVEQVL+LFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI
Sbjct: 1   MDVEQVLDLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG 120
           SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSK+IAGEEPPEKSRRVVIRQKRKQ RRIG
Sbjct: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKEIAGEEPPEKSRRVVIRQKRKQRRRIG 120

Query: 121 GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180
           GRSIR SESRSLSELEFEE+KGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG
Sbjct: 121 GRSIRGSESRSLSELEFEEVKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180

Query: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 235
           GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR
Sbjct: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 234

BLAST of Carg19894-RA vs. NCBI nr
Match: XP_023001638.1 (uncharacterized protein LOC111495710 [Cucurbita maxima])

HSP 1 Score: 446.4 bits (1147), Expect = 1.5e-121
Identity = 229/241 (95.02%), Postives = 231/241 (95.85%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MDVEQVLNLFDS WFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI
Sbjct: 1   MDVEQVLNLFDSFWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG 120
           SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQ RRIG
Sbjct: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQRRRIG 120

Query: 121 GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRD------- 180
           GRSIR SESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRD       
Sbjct: 121 GRSIRGSESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEEEE 180

Query: 181 EEEEELGGGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTV 235
           EEEEELGGGISRPYLSEAWAAME+EEE+KKALVMKWRLPANEIDMKDNLKWWAHAVASTV
Sbjct: 181 EEEEELGGGISRPYLSEAWAAMEQEEEVKKALVMKWRLPANEIDMKDNLKWWAHAVASTV 240

BLAST of Carg19894-RA vs. NCBI nr
Match: XP_023520224.1 (uncharacterized protein LOC111783529 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 439.9 bits (1130), Expect = 1.4e-119
Identity = 223/234 (95.30%), Postives = 227/234 (97.01%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MDVEQVLNLFDS WFEREIFNKHPFPTNPQNPRPENQD DPL+NSPPEE FVPRICPRSI
Sbjct: 1   MDVEQVLNLFDSFWFEREIFNKHPFPTNPQNPRPENQDLDPLENSPPEEAFVPRICPRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG 120
           SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSS+D+ GEE PEKSRRVVIRQKRKQ RRIG
Sbjct: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSQDMGGEEAPEKSRRVVIRQKRKQRRRIG 120

Query: 121 GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180
           GRSIR SESRSLSELEFEE+KGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG
Sbjct: 121 GRSIRGSESRSLSELEFEEVKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180

Query: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 235
           GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR
Sbjct: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 234

BLAST of Carg19894-RA vs. NCBI nr
Match: XP_038895996.1 (uncharacterized protein LOC120084174 [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 1.2e-86
Identity = 182/244 (74.59%), Postives = 200/244 (81.97%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPF-VPRICPRS 60
           MD EQ+LNLFDS WFE EIFNKHPFP+NPQNP+PENQD     NS P+EPF VPR+  RS
Sbjct: 1   MDFEQLLNLFDSFWFEHEIFNKHPFPSNPQNPQPENQD-----NSLPKEPFIVPRLRTRS 60

Query: 61  ISEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVI--RQKRKQSR 120
           ISEDLSSKL+FMS+S+SPDSVLFSPKLQTI SSKDIAG E PE SR+V I  R K +  R
Sbjct: 61  ISEDLSSKLSFMSNSNSPDSVLFSPKLQTIFSSKDIAGAESPENSRKVGIERRPKTESRR 120

Query: 121 RIGGRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGK------ 180
           ++ GR +R SESRSLSELEFEELKGFMDLGFVFSE DK SSLA IVPGLNRLGK      
Sbjct: 121 KLRGRRMRRSESRSLSELEFEELKGFMDLGFVFSEEDKGSSLASIVPGLNRLGKTEEEDI 180

Query: 181 RDEEEEELGGGISRPYLSEAWAAM-EEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVA 235
            +EEE +LGG ISRPYLSEAW AM EEEEELK  L MKW+ P+N+IDMKDNLKWWAHAVA
Sbjct: 181 EEEEERKLGGEISRPYLSEAWEAMEEEEEELKNPLTMKWKFPSNQIDMKDNLKWWAHAVA 239

BLAST of Carg19894-RA vs. ExPASy TrEMBL
Match: A0A6J1EH41 (uncharacterized protein LOC111434058 OS=Cucurbita moschata OX=3662 GN=LOC111434058 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.0e-123
Identity = 229/234 (97.86%), Postives = 232/234 (99.15%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MDVEQVL+LFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI
Sbjct: 1   MDVEQVLDLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG 120
           SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSK+IAGEEPPEKSRRVVIRQKRKQ RRIG
Sbjct: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKEIAGEEPPEKSRRVVIRQKRKQRRRIG 120

Query: 121 GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180
           GRSIR SESRSLSELEFEE+KGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG
Sbjct: 121 GRSIRGSESRSLSELEFEEVKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEELG 180

Query: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 235
           GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR
Sbjct: 181 GGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTVR 234

BLAST of Carg19894-RA vs. ExPASy TrEMBL
Match: A0A6J1KR35 (uncharacterized protein LOC111495710 OS=Cucurbita maxima OX=3661 GN=LOC111495710 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 7.2e-122
Identity = 229/241 (95.02%), Postives = 231/241 (95.85%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MDVEQVLNLFDS WFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI
Sbjct: 1   MDVEQVLNLFDSFWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQSRRIG 120
           SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQ RRIG
Sbjct: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVIRQKRKQRRRIG 120

Query: 121 GRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRD------- 180
           GRSIR SESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRD       
Sbjct: 121 GRSIRGSESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDEEEEEEE 180

Query: 181 EEEEELGGGISRPYLSEAWAAMEEEEELKKALVMKWRLPANEIDMKDNLKWWAHAVASTV 235
           EEEEELGGGISRPYLSEAWAAME+EEE+KKALVMKWRLPANEIDMKDNLKWWAHAVASTV
Sbjct: 181 EEEEELGGGISRPYLSEAWAAMEQEEEVKKALVMKWRLPANEIDMKDNLKWWAHAVASTV 240

BLAST of Carg19894-RA vs. ExPASy TrEMBL
Match: A0A0A0LV26 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G470240 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.3e-78
Identity = 169/254 (66.54%), Postives = 192/254 (75.59%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MD + +LNLFDS WF+R++ N HPFP+NPQ  +P+ QD DPL   P E   +PR+  RSI
Sbjct: 1   MDFDHLLNLFDSFWFQRQVLNNHPFPSNPQILQPQIQDPDPL---PKESFLIPRLRTRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVI--RQKRKQSRR 120
           SEDLSSKL+FMS+S+SPDSVL SPKLQTI SSKDIAG E PE S +V I  R K +  RR
Sbjct: 61  SEDLSSKLSFMSNSNSPDSVLLSPKLQTIFSSKDIAGAESPETSHKVEIERRPKTEYRRR 120

Query: 121 IGGRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRD----- 180
           + GR  R SESRSLSELEFEELKGFMDLGFVFSE DK SSLA IVPGLNRLGKR+     
Sbjct: 121 LRGRRTRRSESRSLSELEFEELKGFMDLGFVFSEEDKGSSLASIVPGLNRLGKREEKGNK 180

Query: 181 ---------EEEEELGGGISRPYLSEAWAAMEEEEE----LKKALVMKWRLPANEIDMKD 235
                    EEE +LGG ISRPYLSEAW A+ EEEE    LK+ L+MKWR P+N+IDMKD
Sbjct: 181 EGEEEEEEKEEERKLGGEISRPYLSEAWEAIAEEEEKEELLKRPLMMKWRFPSNQIDMKD 240

BLAST of Carg19894-RA vs. ExPASy TrEMBL
Match: A0A5A7UPL7 (DUF1685 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002460 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 4.9e-78
Identity = 173/252 (68.65%), Postives = 190/252 (75.40%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFVPRICPRSI 60
           MD EQ+LNLFDS WFER +FNKHPF +N Q   P+ QD D L   P E   +PR+  RSI
Sbjct: 1   MDFEQLLNLFDSFWFERGVFNKHPFLSNLQ---PQIQDPDSL---PKESFIIPRLPTRSI 60

Query: 61  SEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVVI--RQKRKQSRR 120
           SEDLSSKL+FMS S+SPDSVLFSPKLQTI SSKDIAG E PE SR++ I  R K +  RR
Sbjct: 61  SEDLSSKLSFMSSSNSPDSVLFSPKLQTIFSSKDIAGAESPETSRKLEIERRPKTEYRRR 120

Query: 121 IGGRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKR------ 180
             GR  R SESRSLSELEFEELKGFMDLGFVFSE DK SSLA IVPGLNRLGK+      
Sbjct: 121 FRGRRTRRSESRSLSELEFEELKGFMDLGFVFSEEDKGSSLASIVPGLNRLGKKEEKESK 180

Query: 181 ------DEEEEELGGGISRPYLSEAWAAMEEEEE----LKKALVMKWRLPANEIDMKDNL 235
                 +EEE +LGG ISRPYLSEAW AMEEEEE     KK L+MKWR P+N+IDMKDNL
Sbjct: 181 EEEEEEEEEERKLGGEISRPYLSEAWEAMEEEEEKEELAKKPLMMKWRFPSNQIDMKDNL 240

BLAST of Carg19894-RA vs. ExPASy TrEMBL
Match: A0A6J1CEZ9 (uncharacterized protein LOC111010901 OS=Momordica charantia OX=3673 GN=LOC111010901 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.6e-74
Identity = 166/244 (68.03%), Postives = 187/244 (76.64%), Query Frame = 0

Query: 1   MDVEQVLNLFDSLWFEREIFNKHPFPTNPQNPRPENQDRDPLKNSPPEEPFV-PRICPRS 60
           MDVEQ+LNLFDSLWFER IFN+   P  P NP  ENQ++ PLKNSPP  P V PRI  RS
Sbjct: 1   MDVEQILNLFDSLWFERGIFNQ---PLLPSNPERENQEK-PLKNSPPPSPEVLPRIHTRS 60

Query: 61  ISEDLSSKLTFMSDSSSPDSVLFSPKLQTILSSKDIAGEEPPEKSRRVV-----IRQKRK 120
           ISEDLSSKL+FMS+S+SPDS+LFSPKLQTI SSKDIA  E PE +R+ +        +++
Sbjct: 61  ISEDLSSKLSFMSNSNSPDSILFSPKLQTIFSSKDIARTESPENNRKEIRPELETEPRKR 120

Query: 121 QSRRIGGRSIRASESRSLSELEFEELKGFMDLGFVFSEGDKSSSLAWIVPGLNRLGKRDE 180
              R  GR  R  ES+SLSELEFEELKGFMDLGFVFSE DK SSLA I+PGLNRL K++ 
Sbjct: 121 NKGRGRGRRRRWPESKSLSELEFEELKGFMDLGFVFSEEDKDSSLASIIPGLNRLKKKEH 180

Query: 181 EEEELG--GGISRPYLSEAWAAMEEEEELKKALVMKWRLPA--NEIDMKDNLKWWAHAVA 235
           EEEE G  G ISRPYLSEAW AME+E ELKK  +M+W  PA  NEIDMKDNLKWWAH VA
Sbjct: 181 EEEEEGEEGEISRPYLSEAWEAMEKENELKKPPLMEWSFPALGNEIDMKDNLKWWAHTVA 240

BLAST of Carg19894-RA vs. TAIR 10
Match: AT2G42760.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1685 (InterPro:IPR012881); Has 170 Blast hits to 164 proteins in 34 species: Archae - 0; Bacteria - 1; Metazoa - 26; Fungi - 10; Plants - 107; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink). )

HSP 1 Score: 138.3 bits (347), Expect = 8.1e-33
Identity = 109/270 (40.37%), Postives = 154/270 (57.04%), Query Frame = 0

Query: 4   EQVLNLFDSLWFEREIFNKHPFPTNPQNPR--------PENQDRDPLKNSPPEEPFVPRI 63
           E++L LF+  W ER IF K     N ++           E ++ + LKN P     V  +
Sbjct: 4   EELLKLFEQNWSERPIFKKDKENLNGKSREKRGEKEILEERREEEALKNFP-----VSFL 63

Query: 64  CPRSISED----LSSKLTFMSDSS-----SPDSVL----FSPKLQTILSSKDIAGEEPPE 123
             R++S++     SSK +  S SS     SP SVL       KLQTILS K++      E
Sbjct: 64  VERAMSDETMMTTSSKTSLFSSSSDDLFLSPRSVLPVKPTPMKLQTILSGKEVNAFTIAE 123

Query: 124 KSRRVVIRQKRKQSRRIGGRS-IRASESRSLSELEFEELKGFMDLGFVFSEGD-KSSSLA 183
           + R  ++ +K +Q ++   +S +R  + +S+S+LE+EELKGFMDLGFVFSE D K S L 
Sbjct: 124 RER--LLSEKEEQRKKKKKKSNVRTRKGKSMSDLEYEELKGFMDLGFVFSEDDHKDSDLV 183

Query: 184 WIVPGLNRLGKRD----------EEEEELGGG-ISRPYLSEAWAAMEEEEELKKAL-VMK 235
            I+PGL RL K+D          EEE+++GG   +RPYLSEAW      +  K+    +K
Sbjct: 184 SILPGLQRLVKKDDGVTKEEEEEEEEDKIGGNRAARPYLSEAWDHCGGRKGKKQITPEIK 243