Carg27609 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27609
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Glycoprotein membrane precursor GPI-anchored
Location	Carg_Chr10: 7534022 .. 7536662 (+)
RNA-Seq Expression	Carg27609
Synteny	Carg27609

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATGTTCTTCGCCTCTTTTTATTGCTTTGGGTTCGCAGTTCATTTGTGAATTGTTTCCCACAAATATGAGAGTGTTTGCTTTGTTCTTCTTTGCTGTCATCGCCACCTTCCTCCTTCTGCCCAATGCTGTTCTTTGTGACGGTATGATTTTCATCGTTATTTTTCTTTTAACGTTTGTAGCTATTCATCGAAGAATATTGCATGTTGTAATCGCTTATTTTGATATGACGTTTATGTATCATTCAATTTGATAGCTTAAACAGATAAGATTAAAGGTGTTTACGGGATAGGAGGGGTAGGAACGGTAATCCTTCTTCATCCTCGTTCTTCGAAGTTTCTCATTTATTTTCGTATGGATTGAATTTATCCCGAAGAATTTTTTTCCCTTTTTATATATTTTTTCAAAAATATATACCATACAAAAAAATTTAATAATATTTTAGAAATCAAAATATAATATAATTTCTAAGAACTCAAATTTCAATATTACAATTACTTATTCCACACTTCATTTAATGGACGGGGCAAAGAATAGTATCCATGTTTCGAGTCCTATTTAGCTACTTGAGATATATATCTCCACTCCCTTTCTCATTCTCAGTTTAAAAGTAGTTTTTGAAATAGTGTAAAAAGAGGTGCTTCGAATCATTACTATTTGACTCGAACATGTTATAAAAAAGTCGAGGCATTTCGAATTGTTTGTTGACACCGACAAAGTCAAGGAAATGTGTAAAATTATTTCAATATTGGACCTTGGACATATAATAGTTCCGGATCGAATATCTTTAGGATCTTTGGTAGATTTGGTGAGATCTCACATCAGTTGAAGAGGGAACGAAACATTCTTTGTAAGTGTATGAAAATCTCTCTCTATCAAACACGTTTTAAAAACCTTGGGAGAAGTCCGAAAGAGAAAGCCCAAAGAGGACAATATCGGTTAACGGTGGGCTTGGGTTGGGGCGTTACAAATGGTACCAGAACCAAATAACGAACGGTGAGCAAGAAAGGACGCTGAGCCCCAAAAGGAGTGAACATCGGGTAGTGTCCCAGCAAGGAAGTTGGGCCCTAACAATGTGCTAGGGAGAACATTGGGCCTCGAGGGGGGAACTAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCATACGTGTTTTAAAAACCTTGATGGGTAGTCTCCCAAAGAAAACAATATCGGCTAGCGGTGAGCCCTAACAGTGTGTTAGGGAGAACATTGGGCCTCGAGAGGGGAACTAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCATACGTGTTTTAAAAACCTTGATGGGTAGTCTCCCAAAGAAAACAATATCGACTAGCGGTGAGTCCTAACAGTGTGTTAGGGAGAACATTGGGCCTCGAGAGGGGAACTAAGCATTCTTTATAAGGGTGTGGGAAACCTCTCTCTAGCGTACGTGTTTTTAAAAACCTCGATGGGTAAAAACAATATCGGCGAGTGTGTTAGGGTGAACATTGGGCCTCGAGAGGGGAACTAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCATACGTGTTTTAAAAACCTTGATGGGTAGTCTCCCAAAGAAAACAATATCGACTAGCGGTGAGTCCTAACAGTGTGTTAGGGAGAACATTGGGCCTCGAGAGGGGAACTAAGTATTTTTTATAAGGGTGTGGAAACCTCTCTCTAGCGTACGTGTTTTAAAAACTTCGATGGGAAGTCTCCCAGAGAAAAACAATATCCGCTAGCGGTGGATTTAGGCCGTTCGAGGTAGATCTCGACAAATAAATTATAGCGAATTTAATTAGTGTTTCATATTGTTAATGAAGTTACATGCACACCGCACTAAACATAACCAATGCCTCACATGGCTTAGTTTTCTTGTGCAGTGAAAGATACAGAGATTCTAAACGCCATCAACAATTACAGACAATCAAAGCACCTATCTAATCTTACTCATAACAAAAATGCAGCATGTTTAGCAAGCAGGTTCGTTTATAAGCTAAGAGATGAGCCTTGTTCAAGTCCAGATGATTTCCACAAAGAAATCAACTCTGAAAATACCCTTGCAGATTTTCTTAAGCTCTTGAAGAAGTGTCACATAGCCTACAACTCGACCGTTGATGGCATCATGCTCCCCGCTTGCGTCCCCCACCTCGATCCCAAGTCCGTCGCCGATAACTATACCAAATCCCATGACGTCGATTATATTAACAGTAAGAACTTTACGGGTGCTGGGGTCGGGACGTCTGACGATTGGATCGTCCTCGTTCTTAGCACCAATACGTCGACGGGGAACTTTGCTAACGGTGGATCTTCGTCTTTGGTTGTCGCCCGGGGTGGTTGCATTGGTATCATGGTTGGTTTCTTAGGGTTGTTTGTTCTTTTGTTATTTTGATGAGGTGAGGGAAAAGGCTTTTGGGTTTGGGTTTAAAGCATTGGTAACCTAAATAACACAATTGTTTTACAACTCGATCCTACTTAACCCTATCGGGTTGGATTGAATTAGGTTGTTTTTAAAAATAAATTATACCCACGATATATAATCATTCGGGACAATTTTAACGAAAATATATCACAAACCCTAAAAAAAATCACAATTTGTAAGGATTTCAAACAAGG

mRNA sequence

TATGTTCTTCGCCTCTTTTTATTGCTTTGGGTTCGCAGTTCATTTGTGAATTGTTTCCCACAAATATGAGAGTGTTTGCTTTGTTCTTCTTTGCTGTCATCGCCACCTTCCTCCTTCTGCCCAATGCTGTTCTTTGTGACGTGAAAGATACAGAGATTCTAAACGCCATCAACAATTACAGACAATCAAAGCACCTATCTAATCTTACTCATAACAAAAATGCAGCATGTTTAGCAAGCAGGTTCGTTTATAAGCTAAGAGATGAGCCTTGTTCAAGTCCAGATGATTTCCACAAAGAAATCAACTCTGAAAATACCCTTGCAGATTTTCTTAAGCTCTTGAAGAAGTGTCACATAGCCTACAACTCGACCGTTGATGGCATCATGCTCCCCGCTTGCGTCCCCCACCTCGATCCCAAGTCCGTCGCCGATAACTATACCAAATCCCATGACGTCGATTATATTAACAGTAAGAACTTTACGGGTGCTGGGGTCGGGACGTCTGACGATTGGATCGTCCTCGTTCTTAGCACCAATACGTCGACGGGGAACTTTGCTAACGGTGGATCTTCGTCTTTGGTTGTCGCCCGGGGTGGTTGCATTGGTATCATGGTTGGTTTCTTAGGGTTGTTTGTTCTTTTGTTATTTTGATGAGGTGAGGGAAAAGGCTTTTGGGTTTGGGTTTAAAGCATTGGTAACCTAAATAACACAATTGTTTTACAACTCGATCCTACTTAACCCTATCGGGTTGGATTGAATTAGGTTGTTTTTAAAAATAAATTATACCCACGATATATAATCATTCGGGACAATTTTAACGAAAATATATCACAAACCCTAAAAAAAATCACAATTTGTAAGGATTTCAAACAAGG

Coding sequence (CDS)

ATGAGAGTGTTTGCTTTGTTCTTCTTTGCTGTCATCGCCACCTTCCTCCTTCTGCCCAATGCTGTTCTTTGTGACGTGAAAGATACAGAGATTCTAAACGCCATCAACAATTACAGACAATCAAAGCACCTATCTAATCTTACTCATAACAAAAATGCAGCATGTTTAGCAAGCAGGTTCGTTTATAAGCTAAGAGATGAGCCTTGTTCAAGTCCAGATGATTTCCACAAAGAAATCAACTCTGAAAATACCCTTGCAGATTTTCTTAAGCTCTTGAAGAAGTGTCACATAGCCTACAACTCGACCGTTGATGGCATCATGCTCCCCGCTTGCGTCCCCCACCTCGATCCCAAGTCCGTCGCCGATAACTATACCAAATCCCATGACGTCGATTATATTAACAGTAAGAACTTTACGGGTGCTGGGGTCGGGACGTCTGACGATTGGATCGTCCTCGTTCTTAGCACCAATACGTCGACGGGGAACTTTGCTAACGGTGGATCTTCGTCTTTGGTTGTCGCCCGGGGTGGTTGCATTGGTATCATGGTTGGTTTCTTAGGGTTGTTTGTTCTTTTGTTATTTTGA

Protein sequence

MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIGIMVGFLGLFVLLLF

Homology

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BLAST of Carg27609 vs. NCBI nr
Match: KAG7023953.1 (putative GPI-anchored protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.9 bits (982), Expect = 1.7e-102
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF
Sbjct: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 181 IMVGFLGLFVLLLF 194

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: XP_022961524.1 (uncharacterized GPI-anchored protein At5g19250-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 382.9 bits (982), Expect = 1.7e-102
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF
Sbjct: 32  MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 91

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 92  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 151

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 152 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 211

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 212 IMVGFLGLFVLLLF 225

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: KAG6590391.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.1 bits (967), Expect = 9.2e-101
Identity = 191/194 (98.45%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQ KHLSNLTHNKNAACLASRF
Sbjct: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQLKHLSNLTHNKNAACLASRF 60

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           A NYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGC+G
Sbjct: 121 ASNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCMG 180

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 181 IMVGFLGLFVLLLF 194

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: XP_023516540.1 (uncharacterized GPI-anchored protein At5g19250-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.9 bits (951), Expect = 6.6e-99
Identity = 187/194 (96.39%), Postives = 191/194 (98.45%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFF V+ATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF
Sbjct: 1   MRVFALFFFVVVATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           + NYTKS DV+YI+SKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 121 SSNYTKSRDVEYISSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 181 IMVGFLGLFVLLLF 194

[Close]

BLAST of Carg27609 vs. NCBI nr
Match: XP_022968795.1 (uncharacterized GPI-anchored protein At3g06035-like [Cucurbita maxima])

HSP 1 Score: 349.0 bits (894), Expect = 2.7e-92
Identity = 179/194 (92.27%), Postives = 186/194 (95.88%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFAL FFAVIATFLLLPNAVLCDV+D EILNAINNYRQ K LSN T+NKNAACLASRF
Sbjct: 1   MRVFAL-FFAVIATFLLLPNAVLCDVRDIEILNAINNYRQKKSLSNFTYNKNAACLASRF 60

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDF+KEINSENTLADF KLLKKCHIAYNSTVDGIMLPACVPHLDP+SV
Sbjct: 61  VYKLRDEPCSSPDDFNKEINSENTLADFHKLLKKCHIAYNSTVDGIMLPACVPHLDPESV 120

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ++NYTKS DVDYINSKNFTGAGVGTSDDWIVL+LSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 121 SNNYTKSRDVDYINSKNFTGAGVGTSDDWIVLILSTNTSTGNFANGGSSSLVVARGGCIG 180

Query: 181 IMVGFLGLFVLLLF 195
           IM GFLGLFVLLLF
Sbjct: 181 IMAGFLGLFVLLLF 193

[Close]

BLAST of Carg27609 vs. ExPASy Swiss-Prot
Match: P59833 (Uncharacterized GPI-anchored protein At5g19250 OS=Arabidopsis thaliana OX=3702 GN=At5g19250 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-22
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTE--ILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL D  D E  +L  IN+YR S +L+ L HN NA CLA 
Sbjct: 1   MAISKLHLLFLLSVFLSLHPLVLSDTADEEDVLLTGINSYRASLNLTTLIHNHNAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
               + +++PC++         +     +   LL KC +    T DG +LPACVP+LDP 
Sbjct: 61  EIADQFKNQPCTNTTGSASVPGTTPGFPNLPNLLSKCRLNPTVTRDGAILPACVPNLDPS 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSS-SLVVARGG 180
            V  N+T S     +N   FTG G+G+ D+WIV+VL+T+T  G+++   +S +      G
Sbjct: 121 LVLTNFTMSQYSKDLNDSKFTGIGIGSDDNWIVVVLTTSTPEGSYSPASNSGAFAFGVNG 180

Query: 181 CIGIMVGFLGLFVLLLF 195
            +   + FL LF   +F
Sbjct: 181 LVSSSLMFL-LFCFFMF 196

[Close]

BLAST of Carg27609 vs. ExPASy Swiss-Prot
Match: Q84MC0 (Uncharacterized GPI-anchored protein At3g06035 OS=Arabidopsis thaliana OX=3702 GN=At3g06035 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-22
Identity = 67/196 (34.18%), Postives = 110/196 (56.12%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEI-LNAINNYRQSKHLSNLTHNKNAACLASR 60
           M +  L     ++  L L   VL D  + +I L  IN+YR +++L+ L+ N+NA CLA  
Sbjct: 1   MAIDKLPLLLFLSILLCLNRPVLSDTDEEDILLTGINSYRTTQNLTILSKNENAECLADE 60

Query: 61  FVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKS 120
              + +++PC++         +E   A++ ++L KCH+  + T DG ++PACVP L+   
Sbjct: 61  IADQFKNKPCTNDTGSATVPGTEPQFANYPQILAKCHLNVSDTRDGSIMPACVPRLESNL 120

Query: 121 VADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCI 180
           V  N+TKS     +N   FTG G+G  DDWIV+VL+TNT  G+++   + +   + G   
Sbjct: 121 VLTNFTKSQYSMSLNDSKFTGIGIGKEDDWIVVVLTTNTPEGSYST-ATPTKQESNGFTF 180

Query: 181 GI-MVGFLGLFVLLLF 195
           GI +V +L +F+   F
Sbjct: 181 GIGLVSYLVIFMYSSF 195

[Close]

BLAST of Carg27609 vs. ExPASy Swiss-Prot
Match: Q84VZ5 (Uncharacterized GPI-anchored protein At5g19240 OS=Arabidopsis thaliana OX=3702 GN=At5g19240 PE=2 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 4.1e-19
Identity = 61/165 (36.97%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 11  VIATFLLLPNAVLCDVKDTEIL-NAINNYRQSKHLSNLTHNKNAACLASRFVYKLRDEPC 70
           +++ FL L   VL +     +L    N YR+S +L+NL  NKNA CLA   V +L+++PC
Sbjct: 12  LLSVFLSLHRPVLSNTDGENLLFTVFNQYRESVNLTNLKKNKNAECLADEVVDQLQNQPC 71

Query: 71  SSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVP--HLDP--KSVADNYT 130
           ++         ++  + +F  LL KC +  N T DG++L  C P  H  P   S A+  T
Sbjct: 72  TNTTGSASVPGTDPGIPNFPNLLAKCRLNTNVTRDGLILQVCAPKHHSTPDLSSFANVLT 131

Query: 131 KSHDVDYINSKNFTGAGVGTSDD--WIVLVLSTNTSTGNFANGGS 169
           K+     +N   FTGAGVG   D  W+V VL+TNT  G+++N G+
Sbjct: 132 KN-----LNDSKFTGAGVGIDSDGIWLVTVLTTNTPGGSYSNSGA 171

[Close]

BLAST of Carg27609 vs. ExPASy Swiss-Prot
Match: Q8GUL8 (Uncharacterized GPI-anchored protein At5g19230 OS=Arabidopsis thaliana OX=3702 GN=At5g19230 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.4e-13
Identity = 62/198 (31.31%), Postives = 92/198 (46.46%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVL--CDVKDTEILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL   D +D  +L   N YR   +L  LT N+NA CLA 
Sbjct: 1   MAISKLHLLCLLSVFLSLHRLVLSGTDGEDDLLLTVFNKYRTGLNLKTLTKNENAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
             V +L+++PC++ +      NS     +   LL KC +      DG+++  C P  D  
Sbjct: 61  EVVDQLKNQPCTNTN------NSAPVPGN--NLLAKCSLNTTVVRDGVIMQVCFPKHDKN 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGC 180
               N+  S  +  +N    TG G+G+ D W+V++L+TNT       GG S L     G 
Sbjct: 121 PDLSNF-NSVVLKNLNDSKITGIGIGSGDIWVVVILTTNT-----PEGGYSLLTTTNSGA 180

Query: 181 IGIMVGFL--GLFVLLLF 195
               V  L    F+ LLF
Sbjct: 181 YAFGVNGLVSSSFLFLLF 184

[Close]

BLAST of Carg27609 vs. ExPASy TrEMBL
Match: A0A6J1HEB1 (uncharacterized GPI-anchored protein At5g19250-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462076 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 8.1e-103
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF
Sbjct: 32  MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 91

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 92  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 151

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 152 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 211

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 212 IMVGFLGLFVLLLF 225

[Close]

BLAST of Carg27609 vs. ExPASy TrEMBL
Match: A0A6J1HY77 (uncharacterized GPI-anchored protein At3g06035-like OS=Cucurbita maxima OX=3661 GN=LOC111467926 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.3e-92
Identity = 179/194 (92.27%), Postives = 186/194 (95.88%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFAL FFAVIATFLLLPNAVLCDV+D EILNAINNYRQ K LSN T+NKNAACLASRF
Sbjct: 1   MRVFAL-FFAVIATFLLLPNAVLCDVRDIEILNAINNYRQKKSLSNFTYNKNAACLASRF 60

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDF+KEINSENTLADF KLLKKCHIAYNSTVDGIMLPACVPHLDP+SV
Sbjct: 61  VYKLRDEPCSSPDDFNKEINSENTLADFHKLLKKCHIAYNSTVDGIMLPACVPHLDPESV 120

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ++NYTKS DVDYINSKNFTGAGVGTSDDWIVL+LSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 121 SNNYTKSRDVDYINSKNFTGAGVGTSDDWIVLILSTNTSTGNFANGGSSSLVVARGGCIG 180

Query: 181 IMVGFLGLFVLLLF 195
           IM GFLGLFVLLLF
Sbjct: 181 IMAGFLGLFVLLLF 193

[Close]

BLAST of Carg27609 vs. ExPASy TrEMBL
Match: A0A6J1HCG9 (uncharacterized GPI-anchored protein At3g06035-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462076 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 7.9e-82
Identity = 166/194 (85.57%), Postives = 166/194 (85.57%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRF 60
           MRVFALFFFAVIATFLLLPNAVLCD                            ACLASRF
Sbjct: 32  MRVFALFFFAVIATFLLLPNAVLCD----------------------------ACLASRF 91

Query: 61  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 120
           VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV
Sbjct: 92  VYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 151

Query: 121 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 180
           ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG
Sbjct: 152 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 197

Query: 181 IMVGFLGLFVLLLF 195
           IMVGFLGLFVLLLF
Sbjct: 212 IMVGFLGLFVLLLF 197

[Close]

BLAST of Carg27609 vs. ExPASy TrEMBL
Match: A0A5D3CFH2 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003820 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.2e-67
Identity = 135/196 (68.88%), Postives = 162/196 (82.65%), Query Frame = 0


Query: 2   RVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFV 61
           R+F LFFF VI+ FLL PN VLCDVKD  IL+AIN YRQSK+LS  ++NKNAACLA + V
Sbjct: 7   RLFTLFFFVVISIFLLHPNVVLCDVKDDNILSAINKYRQSKNLSEFSYNKNAACLAGKLV 66

Query: 62  YKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVA 121
           YKLRDEPCSS ++F+KEI+SE  LADF KLL+KCHIAYNS++DGI+LP+CVP LDP SV 
Sbjct: 67  YKLRDEPCSSAENFNKEISSETKLADFPKLLRKCHIAYNSSIDGIILPSCVPGLDPTSVT 126

Query: 122 DNYTKSHDVDYINSKNFTGAGVGTSDD-WIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 181
            NYT SHD +YIN +N+TGAGVGT +D W+VL+LSTNTSTGN+ + GSSSLVVA GG +G
Sbjct: 127 SNYTHSHDAEYINDQNYTGAGVGTIEDAWVVLILSTNTSTGNYDSSGSSSLVVAGGGRVG 186

Query: 182 IMVGFLGLFV--LLLF 195
           +MV  LG+FV  LL F
Sbjct: 187 VMVALLGMFVSSLLFF 202

[Close]

BLAST of Carg27609 vs. ExPASy TrEMBL
Match: A0A1S3BQD0 (uncharacterized GPI-anchored protein At5g19250-like OS=Cucumis melo OX=3656 GN=LOC103492177 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.2e-67
Identity = 135/196 (68.88%), Postives = 162/196 (82.65%), Query Frame = 0


Query: 2   RVFALFFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFV 61
           R+F LFFF VI+ FLL PN VLCDVKD  IL+AIN YRQSK+LS  ++NKNAACLA + V
Sbjct: 7   RLFTLFFFVVISIFLLHPNVVLCDVKDDNILSAINKYRQSKNLSEFSYNKNAACLAGKLV 66

Query: 62  YKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSVA 121
           YKLRDEPCSS ++F+KEI+SE  LADF KLL+KCHIAYNS++DGI+LP+CVP LDP SV 
Sbjct: 67  YKLRDEPCSSAENFNKEISSETKLADFPKLLRKCHIAYNSSIDGIILPSCVPGLDPTSVT 126

Query: 122 DNYTKSHDVDYINSKNFTGAGVGTSDD-WIVLVLSTNTSTGNFANGGSSSLVVARGGCIG 181
            NYT SHD +YIN +N+TGAGVGT +D W+VL+LSTNTSTGN+ + GSSSLVVA GG +G
Sbjct: 127 SNYTHSHDAEYINDQNYTGAGVGTIEDAWVVLILSTNTSTGNYDSSGSSSLVVAGGGRVG 186

Query: 182 IMVGFLGLFV--LLLF 195
           +MV  LG+FV  LL F
Sbjct: 187 VMVALLGMFVSSLLFF 202

[Close]

BLAST of Carg27609 vs. TAIR 10
Match: AT5G19250.1 (Glycoprotein membrane precursor GPI-anchored )

HSP 1 Score: 107.8 bits (268), Expect = 9.7e-24
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTE--ILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL D  D E  +L  IN+YR S +L+ L HN NA CLA 
Sbjct: 1   MAISKLHLLFLLSVFLSLHPLVLSDTADEEDVLLTGINSYRASLNLTTLIHNHNAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
               + +++PC++         +     +   LL KC +    T DG +LPACVP+LDP 
Sbjct: 61  EIADQFKNQPCTNTTGSASVPGTTPGFPNLPNLLSKCRLNPTVTRDGAILPACVPNLDPS 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSS-SLVVARGG 180
            V  N+T S     +N   FTG G+G+ D+WIV+VL+T+T  G+++   +S +      G
Sbjct: 121 LVLTNFTMSQYSKDLNDSKFTGIGIGSDDNWIVVVLTTSTPEGSYSPASNSGAFAFGVNG 180

Query: 181 CIGIMVGFLGLFVLLLF 195
            +   + FL LF   +F
Sbjct: 181 LVSSSLMFL-LFCFFMF 196

[Close]

BLAST of Carg27609 vs. TAIR 10
Match: AT3G06035.1 (Glycoprotein membrane precursor GPI-anchored )

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-23
Identity = 67/196 (34.18%), Postives = 110/196 (56.12%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVLCDVKDTEI-LNAINNYRQSKHLSNLTHNKNAACLASR 60
           M +  L     ++  L L   VL D  + +I L  IN+YR +++L+ L+ N+NA CLA  
Sbjct: 1   MAIDKLPLLLFLSILLCLNRPVLSDTDEEDILLTGINSYRTTQNLTILSKNENAECLADE 60

Query: 61  FVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKS 120
              + +++PC++         +E   A++ ++L KCH+  + T DG ++PACVP L+   
Sbjct: 61  IADQFKNKPCTNDTGSATVPGTEPQFANYPQILAKCHLNVSDTRDGSIMPACVPRLESNL 120

Query: 121 VADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGCI 180
           V  N+TKS     +N   FTG G+G  DDWIV+VL+TNT  G+++   + +   + G   
Sbjct: 121 VLTNFTKSQYSMSLNDSKFTGIGIGKEDDWIVVVLTTNTPEGSYST-ATPTKQESNGFTF 180

Query: 181 GI-MVGFLGLFVLLLF 195
           GI +V +L +F+   F
Sbjct: 181 GIGLVSYLVIFMYSSF 195

[Close]

BLAST of Carg27609 vs. TAIR 10
Match: AT1G54860.1 (Glycoprotein membrane precursor GPI-anchored )

HSP 1 Score: 97.1 bits (240), Expect = 1.7e-20
Identity = 58/194 (29.90%), Postives = 102/194 (52.58%), Query Frame = 0


Query: 7   FFFAVIATFLLLPNAVLCDVKDTEILNAINNYRQSKHLSNLTHNKNAACLASRFVYKLRD 66
           F+   +  F L+   V+ +  +  +L  +N+YR ++ +     N+ A C+A     KL D
Sbjct: 11  FYIIHLLIFSLISTCVVSNQAEDNLLQGLNSYRTAQRVPPFAKNEKADCVADEIADKLED 70

Query: 67  EPCSSPDDFHKEINSEN------TLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPKSV 126
           +PC++    H   ++         L ++  +L +C I  N+T DG++LP C+P+  P   
Sbjct: 71  QPCTN----HTTASTVTPGSVPPRLTNYQDILSECKIDPNTTRDGLILPVCIPNRIPTLA 130

Query: 127 ADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANG-----GSSSLVVAR 186
             NYT++    Y+N   + GAGVG+  +W+V+VL+T+T  G+F  G      +S  V+A 
Sbjct: 131 LTNYTQTGYARYLNDSRYVGAGVGSEKEWMVVVLTTSTPGGSFTAGVAAGKATSVRVMAG 190

Query: 187 GGCIGIMVGFLGLF 190
            G +G++   L LF
Sbjct: 191 LGLMGLLFSCLVLF 200

[Close]

BLAST of Carg27609 vs. TAIR 10
Match: AT5G19240.1 (Glycoprotein membrane precursor GPI-anchored )

HSP 1 Score: 96.3 bits (238), Expect = 2.9e-20
Identity = 61/165 (36.97%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 11  VIATFLLLPNAVLCDVKDTEIL-NAINNYRQSKHLSNLTHNKNAACLASRFVYKLRDEPC 70
           +++ FL L   VL +     +L    N YR+S +L+NL  NKNA CLA   V +L+++PC
Sbjct: 12  LLSVFLSLHRPVLSNTDGENLLFTVFNQYRESVNLTNLKKNKNAECLADEVVDQLQNQPC 71

Query: 71  SSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVP--HLDP--KSVADNYT 130
           ++         ++  + +F  LL KC +  N T DG++L  C P  H  P   S A+  T
Sbjct: 72  TNTTGSASVPGTDPGIPNFPNLLAKCRLNTNVTRDGLILQVCAPKHHSTPDLSSFANVLT 131

Query: 131 KSHDVDYINSKNFTGAGVGTSDD--WIVLVLSTNTSTGNFANGGS 169
           K+     +N   FTGAGVG   D  W+V VL+TNT  G+++N G+
Sbjct: 132 KN-----LNDSKFTGAGVGIDSDGIWLVTVLTTNTPGGSYSNSGA 171

[Close]

BLAST of Carg27609 vs. TAIR 10
Match: AT5G19230.1 (Glycoprotein membrane precursor GPI-anchored )

HSP 1 Score: 76.6 bits (187), Expect = 2.4e-14
Identity = 62/198 (31.31%), Postives = 92/198 (46.46%), Query Frame = 0


Query: 1   MRVFALFFFAVIATFLLLPNAVL--CDVKDTEILNAINNYRQSKHLSNLTHNKNAACLAS 60
           M +  L    +++ FL L   VL   D +D  +L   N YR   +L  LT N+NA CLA 
Sbjct: 1   MAISKLHLLCLLSVFLSLHRLVLSGTDGEDDLLLTVFNKYRTGLNLKTLTKNENAECLAD 60

Query: 61  RFVYKLRDEPCSSPDDFHKEINSENTLADFLKLLKKCHIAYNSTVDGIMLPACVPHLDPK 120
             V +L+++PC++ +      NS     +   LL KC +      DG+++  C P  D  
Sbjct: 61  EVVDQLKNQPCTNTN------NSAPVPGN--NLLAKCSLNTTVVRDGVIMQVCFPKHDKN 120

Query: 121 SVADNYTKSHDVDYINSKNFTGAGVGTSDDWIVLVLSTNTSTGNFANGGSSSLVVARGGC 180
               N+  S  +  +N    TG G+G+ D W+V++L+TNT       GG S L     G 
Sbjct: 121 PDLSNF-NSVVLKNLNDSKITGIGIGSGDIWVVVILTTNT-----PEGGYSLLTTTNSGA 180

Query: 181 IGIMVGFL--GLFVLLLF 195
               V  L    F+ LLF
Sbjct: 181 YAFGVNGLVSSSFLFLLF 184

The following BLAST results are available for this feature:

BLAST of Carg27609 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023953.1	1.7e-102	100.00	putative GPI-anchored protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022961524.1	1.7e-102	100.00	uncharacterized GPI-anchored protein At5g19250-like isoform X1 [Cucurbita moscha...	[more]
KAG6590391.1	9.2e-101	98.45	putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023516540.1	6.6e-99	96.39	uncharacterized GPI-anchored protein At5g19250-like [Cucurbita pepo subsp. pepo]	[more]
XP_022968795.1	2.7e-92	92.27	uncharacterized GPI-anchored protein At3g06035-like [Cucurbita maxima]	[more]

BLAST of Carg27609 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P59833	1.4e-22	35.03	Uncharacterized GPI-anchored protein At5g19250 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q84MC0	1.8e-22	34.18	Uncharacterized GPI-anchored protein At3g06035 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q84VZ5	4.1e-19	36.97	Uncharacterized GPI-anchored protein At5g19240 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q8GUL8	3.4e-13	31.31	Uncharacterized GPI-anchored protein At5g19230 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of Carg27609 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HEB1	8.1e-103	100.00	uncharacterized GPI-anchored protein At5g19250-like isoform X1 OS=Cucurbita mosc...	[more]
A0A6J1HY77	1.3e-92	92.27	uncharacterized GPI-anchored protein At3g06035-like OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1HCG9	7.9e-82	85.57	uncharacterized GPI-anchored protein At3g06035-like isoform X2 OS=Cucurbita mosc...	[more]
A0A5D3CFH2	3.2e-67	68.88	Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BQD0	3.2e-67	68.88	uncharacterized GPI-anchored protein At5g19250-like OS=Cucumis melo OX=3656 GN=L...	[more]

BLAST of Carg27609 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19250.1	9.7e-24	35.03	Glycoprotein membrane precursor GPI-anchored	[more]
AT3G06035.1	1.3e-23	34.18	Glycoprotein membrane precursor GPI-anchored	[more]
AT1G54860.1	1.7e-20	29.90	Glycoprotein membrane precursor GPI-anchored	[more]
AT5G19240.1	2.9e-20	36.97	Glycoprotein membrane precursor GPI-anchored	[more]
AT5G19230.1	2.4e-14	31.31	Glycoprotein membrane precursor GPI-anchored	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33976	OS07G0645000 PROTEIN	coord: 4..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27609-RA	Carg27609-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane