Carg27258 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27258
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAlcohol dehydrogenase
LocationCarg_Chr09: 6339319 .. 6341453 (+)
RNA-Seq ExpressionCarg27258
SyntenyCarg27258
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGTTCTTCATTTCTCATCACCATTCGCATCAAATCATTCTCAGTTCATCAAAGCTTTCTTTCTACAGATCTCTCTTCTACTATAATCAACGATGTCTAGCACTGCTGGTCAGGTCATCAAATGCAAAGGTAATAGTAACATTTCATTTCGGTATTCGTGAAATTTGGTACGGATATTGGGATTCGATCAATATAGTCTTAAGTTTAGGGATTCGTGTTTGAGTTTTGAATTGATTTGGATGTTTGGATCTTTGTTTTGTTGTAGCTGCTGTGGCTTGGGAGGCTGGAAAGCCACTAGTGATTGAGGAAGTGGAAGTGGCGCCGCCGCAAGCCAATGAAGTTAGAGTGAAGATCCTTTTCACGGCTCTTTGTCACACCGATGTTTATTTCTGGGAAGCGAAGGTAAATTTGTAATCAATGATGAACGAATGTATCACATCGGTTAGGTTTTTGATTTTGTTTGCGTTTTGTAGGGCCAAACTCCTTTGTTTCCTCGCATTTTTGGACATGAAGCTGGAGGGTAAGTAGTGGATTGATTGTTCTGATTTTGATTCTGATTGTGAATCATTTTGTTTGTGATATCGATTTTTGGTTTTTGGATCTGTTTGATTAGTGTTGTTGAGAGTGTTGGAGAAGGCGTGAAAGATCTTCAACCAGGAGATCATGTTCTTCCTGTTTTCACTGGTGAATGTGGCGATTGTCGTCATTGTAAATCAGAAGAGAGCAATATGTGCGATCTTCTTCGAATCAATACTGATCGTGGAGTTATGATCAGTGATGGCAAGACCAGATTCTCCAAAAATGGACAACCAATTCATCATTTCGTTGGAACCTCCACTTTTAGTGAATACACTGTTATTCATTTTGGCTGTTTGGCTAAGATCAATCCTGCTGCTCCTCTTGACAAAGTCTGCGTTCTTAGCTGCGGAATTTCCACAGGTTTTGAACATTTCTTTGTATAATGTTTGGTAGATTCTTGTTTTCATAAGCTGATATTGAATGAATTTGTTTGTGATTGTGAATGAAGGCTTTGGTGCCACTGTGAATGTTGCAAAGCCTAAAAAGGGTCAATCTGTTGCTATCTTTGGCCTTGGAGCTGTTGGGCTTGCTGTAAGTTTATATATCTGTTCTTCACTCGCAGATTCTCGTTTCTATTATCCCCATAATGACACTGTTTTTCAATCTTAGGCTGCTGAAGGGGCAAGAGTTTCCGGAGCATCTAAGATCATTGGTGTTGATTTGAACCCGGCTCGATTCGAAGAAGGTGCAGTCAAATTACTTGTTTTGGATGAATTATCATGTACCATATACTTAATGTATTGATTTGATTTGTTTGATCTTTGCAGCAAAGAAATTTGGTTGCAATGAATTTGTTAACCCAAAGGATCACAGCAAGCCAGTTCAAGAGGTGATTGCTGAGATGACGAACGGAGGAGTCGACCGAAGCATCGAATGCACCGGAAGCATCCAAGCAATGATCTCGGCATTCGAATGCGTTCACGATGTAATTAAGAGTCAATTTGAAATGACGTTATAAATGCTTCTAAACAACGAACACTCATTATTGATTTTGTTTTGAAAACAGGGGTGGGGTGTTGCAGTACTAGTGGGAGTCCCAAACAAAGACGACGCATTCAAAACTCATCCTATGAATTTCCTCAACGAAAGAACTCTAAAGGGTACGTTCTTCGGCAACTACAAGCCTCGATCCGACATTCCCGGGGTCGTCGAGAAGTACCTGAAGAAGGTAATGAAAAATCCTCAAAATGTTCAACCCCCATTGAAGGAATTTGATTTCTGAGGCCTTTTTCTTATTGTTTGTTCTTGATTAGGAGATGGAATTGGAGAAATTCATAACTCATTCAGTGCCGTTCTCTGAGATCAACAAGGCGTTTGATTACATGCTGAAAGGGGAGTCCATTCGATGCATCATTCGCATGGGAGACTGATTTAACAAATTCTGGGGTTAGTTTTATATTTTGGAATAAAGATTATGACTGAAACTGGAAGATCTCCGTTTCCATTTTGTTTTCGTTGTTTTATGTTTCTGGGTGTTCTTCGTGTTCATCATCCAGGGATTGCATCGATGTTATTAATATAAATAAATAAATAGAAAGTTCATTTTTTATTTTTC

mRNA sequence

TCTGTTCTTCATTTCTCATCACCATTCGCATCAAATCATTCTCAGTTCATCAAAGCTTTCTTTCTACAGATCTCTCTTCTACTATAATCAACGATGTCTAGCACTGCTGGTCAGGTCATCAAATGCAAAGCTGCTGTGGCTTGGGAGGCTGGAAAGCCACTAGTGATTGAGGAAGTGGAAGTGGCGCCGCCGCAAGCCAATGAAGTTAGAGTGAAGATCCTTTTCACGGCTCTTTGTCACACCGATGTTTATTTCTGGGAAGCGAAGGGCCAAACTCCTTTGTTTCCTCGCATTTTTGGACATGAAGCTGGAGGTGTTGTTGAGAGTGTTGGAGAAGGCGTGAAAGATCTTCAACCAGGAGATCATGTTCTTCCTGTTTTCACTGGTGAATGTGGCGATTGTCGTCATTGTAAATCAGAAGAGAGCAATATGTGCGATCTTCTTCGAATCAATACTGATCGTGGAGTTATGATCAGTGATGGCAAGACCAGATTCTCCAAAAATGGACAACCAATTCATCATTTCGTTGGAACCTCCACTTTTAGTGAATACACTGTTATTCATTTTGGCTGTTTGGCTAAGATCAATCCTGCTGCTCCTCTTGACAAAGTCTGCGTTCTTAGCTGCGGAATTTCCACAGGCTTTGGTGCCACTGTGAATGTTGCAAAGCCTAAAAAGGGTCAATCTGTTGCTATCTTTGGCCTTGGAGCTGTTGGGCTTGCTGCTGCTGAAGGGGCAAGAGTTTCCGGAGCATCTAAGATCATTGGTGTTGATTTGAACCCGGCTCGATTCGAAGAAGCAAAGAAATTTGGTTGCAATGAATTTGTTAACCCAAAGGATCACAGCAAGCCAGTTCAAGAGGTGATTGCTGAGATGACGAACGGAGGAGTCGACCGAAGCATCGAATGCACCGGAAGCATCCAAGCAATGATCTCGGCATTCGAATGCGTTCACGATGGGTGGGGTGTTGCAGTACTAGTGGGAGTCCCAAACAAAGACGACGCATTCAAAACTCATCCTATGAATTTCCTCAACGAAAGAACTCTAAAGGGTACGTTCTTCGGCAACTACAAGCCTCGATCCGACATTCCCGGGGTCGTCGAGAAGTACCTGAAGAAGGAGATGGAATTGGAGAAATTCATAACTCATTCAGTGCCGTTCTCTGAGATCAACAAGGCGTTTGATTACATGCTGAAAGGGGAGTCCATTCGATGCATCATTCGCATGGGAGACTGATTTAACAAATTCTGGGGTTAGTTTTATATTTTGGAATAAAGATTATGACTGAAACTGGAAGATCTCCGTTTCCATTTTGTTTTCGTTGTTTTATGTTTCTGGGTGTTCTTCGTGTTCATCATCCAGGGATTGCATCGATGTTATTAATATAAATAAATAAATAGAAAGTTCATTTTTTATTTTTC

Coding sequence (CDS)

ATGTCTAGCACTGCTGGTCAGGTCATCAAATGCAAAGCTGCTGTGGCTTGGGAGGCTGGAAAGCCACTAGTGATTGAGGAAGTGGAAGTGGCGCCGCCGCAAGCCAATGAAGTTAGAGTGAAGATCCTTTTCACGGCTCTTTGTCACACCGATGTTTATTTCTGGGAAGCGAAGGGCCAAACTCCTTTGTTTCCTCGCATTTTTGGACATGAAGCTGGAGGTGTTGTTGAGAGTGTTGGAGAAGGCGTGAAAGATCTTCAACCAGGAGATCATGTTCTTCCTGTTTTCACTGGTGAATGTGGCGATTGTCGTCATTGTAAATCAGAAGAGAGCAATATGTGCGATCTTCTTCGAATCAATACTGATCGTGGAGTTATGATCAGTGATGGCAAGACCAGATTCTCCAAAAATGGACAACCAATTCATCATTTCGTTGGAACCTCCACTTTTAGTGAATACACTGTTATTCATTTTGGCTGTTTGGCTAAGATCAATCCTGCTGCTCCTCTTGACAAAGTCTGCGTTCTTAGCTGCGGAATTTCCACAGGCTTTGGTGCCACTGTGAATGTTGCAAAGCCTAAAAAGGGTCAATCTGTTGCTATCTTTGGCCTTGGAGCTGTTGGGCTTGCTGCTGCTGAAGGGGCAAGAGTTTCCGGAGCATCTAAGATCATTGGTGTTGATTTGAACCCGGCTCGATTCGAAGAAGCAAAGAAATTTGGTTGCAATGAATTTGTTAACCCAAAGGATCACAGCAAGCCAGTTCAAGAGGTGATTGCTGAGATGACGAACGGAGGAGTCGACCGAAGCATCGAATGCACCGGAAGCATCCAAGCAATGATCTCGGCATTCGAATGCGTTCACGATGGGTGGGGTGTTGCAGTACTAGTGGGAGTCCCAAACAAAGACGACGCATTCAAAACTCATCCTATGAATTTCCTCAACGAAAGAACTCTAAAGGGTACGTTCTTCGGCAACTACAAGCCTCGATCCGACATTCCCGGGGTCGTCGAGAAGTACCTGAAGAAGGAGATGGAATTGGAGAAATTCATAACTCATTCAGTGCCGTTCTCTGAGATCAACAAGGCGTTTGATTACATGCTGAAAGGGGAGTCCATTCGATGCATCATTCGCATGGGAGACTGA

Protein sequence

MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGCNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEINKAFDYMLKGESIRCIIRMGD
Homology
BLAST of Carg27258 vs. NCBI nr
Match: KAG7025037.1 (Alcohol dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783.1 bits (2021), Expect = 1.1e-222
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG
Sbjct: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMGD
Sbjct: 361 KAFDYMLKGESIRCIIRMGD 380

BLAST of Carg27258 vs. NCBI nr
Match: XP_022937363.1 (alcohol dehydrogenase 1 [Cucurbita moschata])

HSP 1 Score: 782.7 bits (2020), Expect = 1.4e-222
Identity = 379/380 (99.74%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG
Sbjct: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDHSKPVQEVIAEMTNGGVDRS+ECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSVECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMGD
Sbjct: 361 KAFDYMLKGESIRCIIRMGD 380

BLAST of Carg27258 vs. NCBI nr
Match: KAG6592176.1 (Alcohol dehydrogenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 781.9 bits (2018), Expect = 2.4e-222
Identity = 379/380 (99.74%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG
Sbjct: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMEL+KFITHSVPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELDKFITHSVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMGD
Sbjct: 361 KAFDYMLKGESIRCIIRMGD 380

BLAST of Carg27258 vs. NCBI nr
Match: XP_023534902.1 (alcohol dehydrogenase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 781.9 bits (2018), Expect = 2.4e-222
Identity = 378/380 (99.47%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG
Sbjct: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDHSKPVQEVIAEMTNGGVDRS+ECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSVECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKY+KKEMELEKFITHSVPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYMKKEMELEKFITHSVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMGD
Sbjct: 361 KAFDYMLKGESIRCIIRMGD 380

BLAST of Carg27258 vs. NCBI nr
Match: XP_022975027.1 (alcohol dehydrogenase 1 [Cucurbita maxima])

HSP 1 Score: 774.2 bits (1998), Expect = 5.0e-220
Identity = 371/380 (97.63%), Postives = 379/380 (99.74%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKI+FT+LCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKIVFTSLCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGG+VESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGIVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGAR+SGASKIIGVDLNPARFEEAKKFG
Sbjct: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARISGASKIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDHSKPVQEVIAEMTNGGVDRS+ECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSVECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKY+ KEMEL+KFITHSVPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYMNKEMELDKFITHSVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMG+
Sbjct: 361 KAFDYMLKGESIRCIIRMGE 380

BLAST of Carg27258 vs. ExPASy Swiss-Prot
Match: P12886 (Alcohol dehydrogenase 1 OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 2.5e-198
Identity = 325/378 (85.98%), Postives = 356/378 (94.18%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MS+T GQ+IKC+AAVAWEAGKPLVIEEVEVAPPQA EVR+KILFT+LCHTDVYFWEAKGQ
Sbjct: 1   MSNTVGQIIKCRAAVAWEAGKPLVIEEVEVAPPQAGEVRLKILFTSLCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGG+VESVGEGV  L+PGDH LPVFTGECG+C HCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGIVESVGEGVTHLKPGDHALPVFTGECGECPHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVM++D K+RFS  GQP+HHFVGTSTFSEYTV+H GC+AKINP APLDKVC+LSCGI
Sbjct: 121 TDRGVMLNDNKSRFSIKGQPVHHFVGTSTFSEYTVVHAGCVAKINPDAPLDKVCILSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
            TG GAT+NVAKPK G SVAIFGLGAVGLAAAEGAR+SGAS+IIGVDL  +RFE AKKFG
Sbjct: 181 CTGLGATINVAKPKPGSSVAIFGLGAVGLAAAEGARISGASRIIGVDLVSSRFELAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
            NEFVNPK+H KPVQ+VIAEMTNGGVDR++ECTGSIQAMISAFECVHDGWGVAVLVGVP+
Sbjct: 241 VNEFVNPKEHDKPVQQVIAEMTNGGVDRAVECTGSIQAMISAFECVHDGWGVAVLVGVPS 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTF+GNYKPR+D+P VVEKY+K E+ELEKFITH+VPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFYGNYKPRTDLPNVVEKYMKGELELEKFITHTVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRM 379
           KAFDYMLKGESIRCII+M
Sbjct: 361 KAFDYMLKGESIRCIIKM 378

BLAST of Carg27258 vs. ExPASy Swiss-Prot
Match: P13603 (Alcohol dehydrogenase 1 OS=Trifolium repens OX=3899 GN=ADH1 PE=2 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 2.5e-198
Identity = 328/378 (86.77%), Postives = 356/378 (94.18%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MS+TAGQVIKC+AAVAWEAGKPLVIEEVEVAPPQA EVR+KILFT+LCHTDVYFWEAKGQ
Sbjct: 1   MSNTAGQVIKCRAAVAWEAGKPLVIEEVEVAPPQAGEVRLKILFTSLCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGG+VESVGEGV  L+PGDH LPVFTGECG+C HCKSEESNMC+LLRIN
Sbjct: 61  TPLFPRIFGHEAGGIVESVGEGVTHLKPGDHALPVFTGECGECPHCKSEESNMCNLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMI+D K+RFS  GQP+HHFVGTSTFSEYTV+H GC+AKINP APLDKVC+LSCGI
Sbjct: 121 TDRGVMINDNKSRFSIKGQPVHHFVGTSTFSEYTVVHAGCVAKINPDAPLDKVCILSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
            TG GATVNVAKPK G SVAIFGLGAVGLAAAEGAR+SGAS+IIGVDL  +RFE AKKFG
Sbjct: 181 CTGLGATVNVAKPKPGSSVAIFGLGAVGLAAAEGARMSGASRIIGVDLVSSRFELAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
            NEFVNPKDH KPVQ+VIAEMT+GGVDR++ECTGSIQAMISAFECVHDGWGVAVLVGVP 
Sbjct: 241 VNEFVNPKDHDKPVQQVIAEMTDGGVDRAVECTGSIQAMISAFECVHDGWGVAVLVGVPK 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTF+GNYKPR+D+P VVE+Y+K E+ELEKFITHS+PFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFYGNYKPRTDLPNVVEQYMKGELELEKFITHSIPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRM 379
           KAFDYMLKGESIRCIIRM
Sbjct: 361 KAFDYMLKGESIRCIIRM 378

BLAST of Carg27258 vs. ExPASy Swiss-Prot
Match: P25141 (Alcohol dehydrogenase 1 OS=Petunia hybrida OX=4102 GN=ADH1 PE=1 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 1.1e-196
Identity = 326/378 (86.24%), Postives = 355/378 (93.92%), Query Frame = 0

Query: 2   SSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQT 61
           S+TAGQVI+CKAAVAWEAGKPLVIEEVEVAPPQ  EVR+KILFT+LCHTDVYFWEAKGQT
Sbjct: 3   SNTAGQVIRCKAAVAWEAGKPLVIEEVEVAPPQKMEVRLKILFTSLCHTDVYFWEAKGQT 62

Query: 62  PLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINT 121
           PLFPRIFGHEAGG+VESVGEGV DL+PGDHVLPVFTGEC  CRHCKSEESNMCDLLRINT
Sbjct: 63  PLFPRIFGHEAGGIVESVGEGVTDLKPGDHVLPVFTGECQQCRHCKSEESNMCDLLRINT 122

Query: 122 DRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGIS 181
           DRGVMI DG+TRFSK+G+PI+HFVGTSTFSEYTV H GC+ KI+P APLDKVCVLSCGIS
Sbjct: 123 DRGVMIHDGQTRFSKDGKPIYHFVGTSTFSEYTVCHSGCVTKIDPQAPLDKVCVLSCGIS 182

Query: 182 TGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGC 241
           TG GAT+NVAKP KG +VAIFGLGAVGLAAAEGAR++GAS+IIGVDLNP+RF +AKKFG 
Sbjct: 183 TGLGATLNVAKPTKGSTVAIFGLGAVGLAAAEGARIAGASRIIGVDLNPSRFNDAKKFGV 242

Query: 242 NEFVNPKDH-SKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 301
            EFVNPKDH  KPVQ+VIAEMT+GGVDRS+ECTG++ AMISAFECVHDGWGVAVLVGVPN
Sbjct: 243 TEFVNPKDHGDKPVQQVIAEMTDGGVDRSVECTGNVNAMISAFECVHDGWGVAVLVGVPN 302

Query: 302 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 361
           KDDAFKTHPMN LNERTLKGTFFGNYKP+SDIP VV+KY+KKE+ELEKFITH VPFSEIN
Sbjct: 303 KDDAFKTHPMNLLNERTLKGTFFGNYKPKSDIPSVVDKYMKKELELEKFITHQVPFSEIN 362

Query: 362 KAFDYMLKGESIRCIIRM 379
           KAFDYMLKGESIRC+I M
Sbjct: 363 KAFDYMLKGESIRCMITM 380

BLAST of Carg27258 vs. ExPASy Swiss-Prot
Match: P06525 (Alcohol dehydrogenase class-P OS=Arabidopsis thaliana OX=3702 GN=ADH1 PE=1 SV=2)

HSP 1 Score: 684.5 bits (1765), Expect = 6.9e-196
Identity = 318/377 (84.35%), Postives = 355/377 (94.16%), Query Frame = 0

Query: 3   STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP 62
           ST GQ+I+CKAAVAWEAGKPLVIEEVEVAPPQ +EVR+KILFT+LCHTDVYFWEAKGQTP
Sbjct: 2   STTGQIIRCKAAVAWEAGKPLVIEEVEVAPPQKHEVRIKILFTSLCHTDVYFWEAKGQTP 61

Query: 63  LFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTD 122
           LFPRIFGHEAGG+VESVGEGV DLQPGDHVLP+FTGECG+CRHC SEESNMCDLLRINT+
Sbjct: 62  LFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPIFTGECGECRHCHSEESNMCDLLRINTE 121

Query: 123 RGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGIST 182
           RG MI DG++RFS NG+PI+HF+GTSTFSEYTV+H G +AKINP APLDKVC++SCG+ST
Sbjct: 122 RGGMIHDGESRFSINGKPIYHFLGTSTFSEYTVVHSGQVAKINPDAPLDKVCIVSCGLST 181

Query: 183 GFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGCN 242
           G GAT+NVAKPKKGQSVAIFGLGAVGL AAEGAR++GAS+IIGVD N  RF++AK+FG  
Sbjct: 182 GLGATLNVAKPKKGQSVAIFGLGAVGLGAAEGARIAGASRIIGVDFNSKRFDQAKEFGVT 241

Query: 243 EFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPNKD 302
           E VNPKDH KP+Q+VIAEMT+GGVDRS+ECTGS+QAMI AFECVHDGWGVAVLVGVP+KD
Sbjct: 242 ECVNPKDHDKPIQQVIAEMTDGGVDRSVECTGSVQAMIQAFECVHDGWGVAVLVGVPSKD 301

Query: 303 DAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEINKA 362
           DAFKTHPMNFLNERTLKGTFFGNYKP++DIPGVVEKY+ KE+ELEKFITH+VPFSEINKA
Sbjct: 302 DAFKTHPMNFLNERTLKGTFFGNYKPKTDIPGVVEKYMNKELELEKFITHTVPFSEINKA 361

Query: 363 FDYMLKGESIRCIIRMG 380
           FDYMLKGESIRCII MG
Sbjct: 362 FDYMLKGESIRCIITMG 378

BLAST of Carg27258 vs. ExPASy Swiss-Prot
Match: P48977 (Alcohol dehydrogenase OS=Malus domestica OX=3750 GN=ADH PE=2 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 4.5e-195
Identity = 323/378 (85.45%), Postives = 353/378 (93.39%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MS+TAGQVI+C+AAVAWEAGKPLVIEEVEVAPPQANEVR+KILFT+LCHTDVYFWEAKGQ
Sbjct: 1   MSNTAGQVIRCRAAVAWEAGKPLVIEEVEVAPPQANEVRIKILFTSLCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
            PLFPRI+GHEAGG+VESVGEGV DL+ GDHVLPVFTGEC DC HCKSEESNMCDLLRIN
Sbjct: 61  NPLFPRIYGHEAGGIVESVGEGVTDLKAGDHVLPVFTGECKDCAHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVM+SDGK+RFS  G+PI+HFVGTSTFSEYTV+H GCLAKINP+APLDKVC+LSCGI
Sbjct: 121 TDRGVMLSDGKSRFSIKGKPIYHFVGTSTFSEYTVVHVGCLAKINPSAPLDKVCLLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STG GAT+NVAKPKKG +VA+FGLGAVGLAAAEGAR+SGAS+IIGVDL+  RFEEAKKFG
Sbjct: 181 STGLGATLNVAKPKKGSTVAVFGLGAVGLAAAEGARLSGASRIIGVDLHSDRFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
             EFVNPK H KPVQEVIAE+TN GVDRSIECTGS +AMISAFECVHDGWGVAVLVGVP+
Sbjct: 241 VTEFVNPKAHEKPVQEVIAELTNRGVDRSIECTGSTEAMISAFECVHDGWGVAVLVGVPH 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KD  FKTHP+NFLNERTLKGTFFGNYK R+DIP VVEKY+ KE+ELEKFITH VPFSEIN
Sbjct: 301 KDAVFKTHPVNFLNERTLKGTFFGNYKTRTDIPSVVEKYMNKELELEKFITHKVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRM 379
           KAF+YMLKGE +RCIIRM
Sbjct: 361 KAFEYMLKGEGLRCIIRM 378

BLAST of Carg27258 vs. ExPASy TrEMBL
Match: A0A6J1FFW1 (alcohol dehydrogenase 1 OS=Cucurbita moschata OX=3662 GN=LOC111443671 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 6.9e-223
Identity = 379/380 (99.74%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG
Sbjct: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDHSKPVQEVIAEMTNGGVDRS+ECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSVECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMGD
Sbjct: 361 KAFDYMLKGESIRCIIRMGD 380

BLAST of Carg27258 vs. ExPASy TrEMBL
Match: A0A6J1IBW6 (alcohol dehydrogenase 1 OS=Cucurbita maxima OX=3661 GN=LOC111473986 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.4e-220
Identity = 371/380 (97.63%), Postives = 379/380 (99.74%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKI+FT+LCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKIVFTSLCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGG+VESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGIVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGAR+SGASKIIGVDLNPARFEEAKKFG
Sbjct: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARISGASKIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDHSKPVQEVIAEMTNGGVDRS+ECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSVECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKY+ KEMEL+KFITHSVPFSEIN
Sbjct: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYMNKEMELDKFITHSVPFSEIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMG+
Sbjct: 361 KAFDYMLKGESIRCIIRMGE 380

BLAST of Carg27258 vs. ExPASy TrEMBL
Match: A0A097BU00 (Alcohol dehydrogenase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 8.1e-216
Identity = 360/380 (94.74%), Postives = 378/380 (99.47%), Query Frame = 0

Query: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQ 60
           MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVR+KILFT+LCHTDVYFWEAKGQ
Sbjct: 1   MSSTAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRLKILFTSLCHTDVYFWEAKGQ 60

Query: 61  TPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRIN 120
           TPLFPRIFGHEAGG+VESVGEGVKDLQPGDHVLPVFTGECG+CRHC+SEESNMCDLLRIN
Sbjct: 61  TPLFPRIFGHEAGGIVESVGEGVKDLQPGDHVLPVFTGECGECRHCQSEESNMCDLLRIN 120

Query: 121 TDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 180
           TDRGVMI+DGKTRFSKNGQPIHHFVGTSTFSEYTV+HFGCLAKINPAAPLDKVCVLSCGI
Sbjct: 121 TDRGVMINDGKTRFSKNGQPIHHFVGTSTFSEYTVVHFGCLAKINPAAPLDKVCVLSCGI 180

Query: 181 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 240
           STG GAT+NVAKPKKGQSVAIFGLGAVGLAAAEGAR++GAS+IIGVDLNPARFEEAKKFG
Sbjct: 181 STGLGATLNVAKPKKGQSVAIFGLGAVGLAAAEGARIAGASRIIGVDLNPARFEEAKKFG 240

Query: 241 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 300
           CNEFVNPKDH+KPVQEVIAEMTNGGVDRS+ECTGSIQAMISAFECVHDGWGVAVLVGVPN
Sbjct: 241 CNEFVNPKDHNKPVQEVIAEMTNGGVDRSVECTGSIQAMISAFECVHDGWGVAVLVGVPN 300

Query: 301 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 360
           KDDAFKTHPMN LNERTLKGTFFGNYKPR+DIPGVVE+YLKKE+ELEKFITHSVPFS+IN
Sbjct: 301 KDDAFKTHPMNLLNERTLKGTFFGNYKPRTDIPGVVEQYLKKELELEKFITHSVPFSDIN 360

Query: 361 KAFDYMLKGESIRCIIRMGD 381
           KAFDYMLKGESIRCIIRMG+
Sbjct: 361 KAFDYMLKGESIRCIIRMGE 380

BLAST of Carg27258 vs. ExPASy TrEMBL
Match: A0A0A0K6K4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G322060 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.2e-213
Identity = 359/376 (95.48%), Postives = 371/376 (98.67%), Query Frame = 0

Query: 3   STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP 62
           STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP
Sbjct: 2   STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP 61

Query: 63  LFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTD 122
           LFPRIFGHEAGG+VESVGEGVKDLQPGDHVLPVFTGECGDCRHC+SEESNMCDLLRINTD
Sbjct: 62  LFPRIFGHEAGGIVESVGEGVKDLQPGDHVLPVFTGECGDCRHCQSEESNMCDLLRINTD 121

Query: 123 RGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGIST 182
           RGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTV+H GCLAKINPAAPLDKVCVLSCGIST
Sbjct: 122 RGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVVHIGCLAKINPAAPLDKVCVLSCGIST 181

Query: 183 GFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGCN 242
           G GAT+NVAKPKKGQSVAIFGLGAVGLAAAEGAR+SGAS+IIGVDLNPARFEEAKKFGCN
Sbjct: 182 GLGATLNVAKPKKGQSVAIFGLGAVGLAAAEGARISGASRIIGVDLNPARFEEAKKFGCN 241

Query: 243 EFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPNKD 302
           EFVNPKDHSKPVQEVIAEMTNGGVDRS+ECTGSIQAMI+AFECVHDGWGVAVLVGVPNKD
Sbjct: 242 EFVNPKDHSKPVQEVIAEMTNGGVDRSVECTGSIQAMIAAFECVHDGWGVAVLVGVPNKD 301

Query: 303 DAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEINKA 362
           DAFKTHPMN LNERTLKGTFFGNYKPRSDIPGVVEKYL KE+ELEKFITH+V FSEINKA
Sbjct: 302 DAFKTHPMNLLNERTLKGTFFGNYKPRSDIPGVVEKYLNKELELEKFITHTVSFSEINKA 361

Query: 363 FDYMLKGESIRCIIRM 379
           F+YMLKGESIRCII+M
Sbjct: 362 FEYMLKGESIRCIIKM 377

BLAST of Carg27258 vs. ExPASy TrEMBL
Match: A0A6J1DNQ0 (alcohol dehydrogenase class-P OS=Momordica charantia OX=3673 GN=LOC111021701 PE=3 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 2.4e-212
Identity = 357/378 (94.44%), Postives = 371/378 (98.15%), Query Frame = 0

Query: 3   STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP 62
           STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP
Sbjct: 2   STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP 61

Query: 63  LFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTD 122
           LFPRIFGHEAGGVVESVGEGV DLQPGDHVLPVFTGECGDCRHC+SEESNMCDLLRINTD
Sbjct: 62  LFPRIFGHEAGGVVESVGEGVTDLQPGDHVLPVFTGECGDCRHCQSEESNMCDLLRINTD 121

Query: 123 RGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGIST 182
           RGVMI+DGKTRFS NGQPIHHFVGTSTFSEYTV+H GCLAKINPAAPLDKVCVLSCGIST
Sbjct: 122 RGVMINDGKTRFSINGQPIHHFVGTSTFSEYTVVHVGCLAKINPAAPLDKVCVLSCGIST 181

Query: 183 GFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGCN 242
           G GAT+NVAKPKKG SVAIFGLGAVGLAAAEGAR+SGAS+IIGVDLNP+RFEEAKKFGCN
Sbjct: 182 GLGATLNVAKPKKGSSVAIFGLGAVGLAAAEGARISGASRIIGVDLNPSRFEEAKKFGCN 241

Query: 243 EFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPNKD 302
           EFVNPKDH+KPVQEVIAEMT+GGVDRS+ECTGSIQAMISAFECVHDGWGVAVLVGVPNKD
Sbjct: 242 EFVNPKDHNKPVQEVIAEMTDGGVDRSVECTGSIQAMISAFECVHDGWGVAVLVGVPNKD 301

Query: 303 DAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEINKA 362
           DAFKTHPMN LNERTLKGTFFGNYKPR+DIPGVVEKYL KE+ELEKFITHSVPFSEINKA
Sbjct: 302 DAFKTHPMNLLNERTLKGTFFGNYKPRTDIPGVVEKYLNKELELEKFITHSVPFSEINKA 361

Query: 363 FDYMLKGESIRCIIRMGD 381
           F+YMLKGESIRCII+MGD
Sbjct: 362 FEYMLKGESIRCIIKMGD 379

BLAST of Carg27258 vs. TAIR 10
Match: AT1G77120.1 (alcohol dehydrogenase 1 )

HSP 1 Score: 684.5 bits (1765), Expect = 4.9e-197
Identity = 318/377 (84.35%), Postives = 355/377 (94.16%), Query Frame = 0

Query: 3   STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP 62
           ST GQ+I+CKAAVAWEAGKPLVIEEVEVAPPQ +EVR+KILFT+LCHTDVYFWEAKGQTP
Sbjct: 2   STTGQIIRCKAAVAWEAGKPLVIEEVEVAPPQKHEVRIKILFTSLCHTDVYFWEAKGQTP 61

Query: 63  LFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTD 122
           LFPRIFGHEAGG+VESVGEGV DLQPGDHVLP+FTGECG+CRHC SEESNMCDLLRINT+
Sbjct: 62  LFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPIFTGECGECRHCHSEESNMCDLLRINTE 121

Query: 123 RGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGIST 182
           RG MI DG++RFS NG+PI+HF+GTSTFSEYTV+H G +AKINP APLDKVC++SCG+ST
Sbjct: 122 RGGMIHDGESRFSINGKPIYHFLGTSTFSEYTVVHSGQVAKINPDAPLDKVCIVSCGLST 181

Query: 183 GFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGCN 242
           G GAT+NVAKPKKGQSVAIFGLGAVGL AAEGAR++GAS+IIGVD N  RF++AK+FG  
Sbjct: 182 GLGATLNVAKPKKGQSVAIFGLGAVGLGAAEGARIAGASRIIGVDFNSKRFDQAKEFGVT 241

Query: 243 EFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPNKD 302
           E VNPKDH KP+Q+VIAEMT+GGVDRS+ECTGS+QAMI AFECVHDGWGVAVLVGVP+KD
Sbjct: 242 ECVNPKDHDKPIQQVIAEMTDGGVDRSVECTGSVQAMIQAFECVHDGWGVAVLVGVPSKD 301

Query: 303 DAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEINKA 362
           DAFKTHPMNFLNERTLKGTFFGNYKP++DIPGVVEKY+ KE+ELEKFITH+VPFSEINKA
Sbjct: 302 DAFKTHPMNFLNERTLKGTFFGNYKPKTDIPGVVEKYMNKELELEKFITHTVPFSEINKA 361

Query: 363 FDYMLKGESIRCIIRMG 380
           FDYMLKGESIRCII MG
Sbjct: 362 FDYMLKGESIRCIITMG 378

BLAST of Carg27258 vs. TAIR 10
Match: AT5G43940.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 4.2e-132
Identity = 213/374 (56.95%), Postives = 281/374 (75.13%), Query Frame = 0

Query: 3   STAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP 62
           +T GQVI CKAAVA+E  KPLVIE+V+VAPPQA EVR+KIL+TALCHTD Y W  K    
Sbjct: 2   ATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHTDAYTWSGKDPEG 61

Query: 63  LFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTD 122
           LFP I GHEA G+VESVGEGV ++Q GDHV+P +  EC +C+ CKS ++N+C  +R  T 
Sbjct: 62  LFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSATG 121

Query: 123 RGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGIST 182
            G+M++D K+RFS NG+PI+HF+GTSTFS+YTV+H   +AKI+P APLDKVC+L CG+ T
Sbjct: 122 VGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPT 181

Query: 183 GFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGCN 242
           G GA  N AK + G +VAIFGLG VGLA AEGA+ +GAS+IIG+D++  ++E AKKFG N
Sbjct: 182 GLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGVN 241

Query: 243 EFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPNKD 302
           EFVNPKDH KP+QEVI ++T+GGVD S EC G++  M +A EC H GWG +V+VGV    
Sbjct: 242 EFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAASG 301

Query: 303 DAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEINKA 362
               T P   +  R  KGT FG +K R+ +P +VEKY+ KE++++++ITH++   EINKA
Sbjct: 302 QEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLTLGEINKA 361

Query: 363 FDYMLKGESIRCII 377
           FD + +G  +RC++
Sbjct: 362 FDLLHEGTCLRCVL 375

BLAST of Carg27258 vs. TAIR 10
Match: AT5G43940.2 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 458.8 bits (1179), Expect = 4.4e-129
Identity = 212/386 (54.92%), Postives = 281/386 (72.80%), Query Frame = 0

Query: 3   STAGQVIKCK------------AAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHT 62
           +T GQVI CK            +AVA+E  KPLVIE+V+VAPPQA EVR+KIL+TALCHT
Sbjct: 2   ATQGQVITCKGTFILDPRTLTISAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHT 61

Query: 63  DVYFWEAKGQTPLFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEE 122
           D Y W  K    LFP I GHEA G+VESVGEGV ++Q GDHV+P +  EC +C+ CKS +
Sbjct: 62  DAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGK 121

Query: 123 SNMCDLLRINTDRGVMISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPL 182
           +N+C  +R  T  G+M++D K+RFS NG+PI+HF+GTSTFS+YTV+H   +AKI+P APL
Sbjct: 122 TNLCGKVRSATGVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPL 181

Query: 183 DKVCVLSCGISTGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNP 242
           DKVC+L CG+ TG GA  N AK + G +VAIFGLG VGLA AEGA+ +GAS+IIG+D++ 
Sbjct: 182 DKVCLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDS 241

Query: 243 ARFEEAKKFGCNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGW 302
            ++E AKKFG NEFVNPKDH KP+QEVI ++T+GGVD S EC G++  M +A EC H GW
Sbjct: 242 KKYETAKKFGVNEFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGW 301

Query: 303 GVAVLVGVPNKDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFI 362
           G +V+VGV        T P   +  R  KGT FG +K R+ +P +VEKY+ KE++++++I
Sbjct: 302 GTSVIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYI 361

Query: 363 THSVPFSEINKAFDYMLKGESIRCII 377
           TH++   EINKAFD + +G  +RC++
Sbjct: 362 THNLTLGEINKAFDLLHEGTCLRCVL 387

BLAST of Carg27258 vs. TAIR 10
Match: AT5G24760.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 431.4 bits (1108), Expect = 7.5e-121
Identity = 197/372 (52.96%), Postives = 271/372 (72.85%), Query Frame = 0

Query: 7   QVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTPLFPR 66
           QVI C AAVAW AG+PLV+EEVEV+PPQ  E+R+K++ T+LC +D+  WE++    L PR
Sbjct: 12  QVITCNAAVAWRAGEPLVMEEVEVSPPQPLEIRIKVVCTSLCRSDLSAWESQS---LLPR 71

Query: 67  IFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTDRGVM 126
           IFGHEA G+VES+GEGV + + GDHVL VFTGECG CRHC S +SNMC +L +   +G+M
Sbjct: 72  IFGHEAAGIVESIGEGVTEFEKGDHVLAVFTGECGSCRHCISGKSNMCQVLGMER-KGLM 131

Query: 127 ISDGKTRFSKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGISTGFGA 186
            SD KTRFS  G+P++H+   S+FSEYTV+H GC  K++P APL K+C+LSCG++ G GA
Sbjct: 132 HSDQKTRFSIKGKPVYHYCAVSSFSEYTVVHSGCAVKVDPLAPLHKICLLSCGVAAGLGA 191

Query: 187 TVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFGCNEFVN 246
             NVA  +KG SV IFGLG VGL+ A+GA++ GA++I+GVD+NPA+ E+AK FG  +F+N
Sbjct: 192 AWNVADVQKGSSVVIFGLGTVGLSVAQGAKLRGAAQILGVDINPAKAEQAKTFGVTDFIN 251

Query: 247 PKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPNKDDAFK 306
             D S+P+ +VI  MT GG D S EC G      +A +   DGWG+ V +GVP       
Sbjct: 252 SNDLSEPIPQVIKRMTGGGADFSFECVGDTGIATTALQSCSDGWGMTVTLGVPKAKPEVS 311

Query: 307 THPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEINKAFDYM 366
            H   FL+ ++LKGT FG +KP+SD+P +++KY+ KE+ +++FITH++ F EINKAF  M
Sbjct: 312 AHYGLFLSGKSLKGTLFGGWKPKSDLPSLIDKYMNKEIMIDEFITHNLSFDEINKAFVLM 371

Query: 367 LKGESIRCIIRM 379
            +G+ +RC++ M
Sbjct: 372 REGKCLRCVLHM 379

BLAST of Carg27258 vs. TAIR 10
Match: AT1G64710.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 412.9 bits (1060), Expect = 2.8e-115
Identity = 191/378 (50.53%), Postives = 273/378 (72.22%), Query Frame = 0

Query: 4   TAGQVIKCKAAVAWEAGKPLVIEEVEVAPPQANEVRVKILFTALCHTDVYFWEAKGQTP- 63
           T G+VI CKAAVAW AG+PLV+E+V+V PPQ  EVR++ILFT++CHTD+  W+ + +   
Sbjct: 19  TQGKVITCKAAVAWGAGEPLVMEDVKVDPPQRLEVRIRILFTSICHTDLSAWKGENEAQR 78

Query: 64  LFPRIFGHEAGGVVESVGEGVKDLQPGDHVLPVFTGECGDCRHCKSEESNMCDLLRINTD 123
            +PRI GHEA G+VESVGEGV+++  GDHVLP+FTGECGDCR CK + +N+C+  R++  
Sbjct: 79  AYPRILGHEAAGIVESVGEGVEEMMAGDHVLPIFTGECGDCRVCKRDGANLCERFRVDPM 138

Query: 124 RGVMISDGKTRF--SKNGQPIHHFVGTSTFSEYTVIHFGCLAKINPAAPLDKVCVLSCGI 183
           + VM++DGKTRF  SK+ +PI+HF+ TSTFSEYTVI   C+ K++P  PL+K+ +LSCG+
Sbjct: 139 KKVMVTDGKTRFFTSKDNKPIYHFLNTSTFSEYTVIDSACVLKVDPLFPLEKISLLSCGV 198

Query: 184 STGFGATVNVAKPKKGQSVAIFGLGAVGLAAAEGARVSGASKIIGVDLNPARFEEAKKFG 243
           STG GA  NVA  +   +VAIFGLGAVGLA AEGAR  GASKIIG+D+NP +F+  ++ G
Sbjct: 199 STGVGAAWNVADIQPASTVAIFGLGAVGLAVAEGARARGASKIIGIDINPDKFQLGREAG 258

Query: 244 CNEFVNPKDHSKPVQEVIAEMTNGGVDRSIECTGSIQAMISAFECVHDGWGVAVLVGVPN 303
            +EF+NPK+  K V E + E+T GGV+ S EC GSI+A+  AF   + G GV V++GV  
Sbjct: 259 ISEFINPKESDKAVHERVMEITEGGVEYSFECAGSIEALREAFLSTNSGVGVTVMLGVHA 318

Query: 304 KDDAFKTHPMNFLNERTLKGTFFGNYKPRSDIPGVVEKYLKKEMELEKFITHSVPFSEIN 363
                  HPM     R++  + FG +KP++ +P  + + L+  + L+ FI+H +PF +IN
Sbjct: 319 SPQLLPIHPMELFQGRSITASVFGGFKPKTQLPFFITQCLQGLLNLDLFISHQLPFHDIN 378

Query: 364 KAFDYMLKGESIRCIIRM 379
           +A   + +G+++RC++ +
Sbjct: 379 EAMQLLHQGKALRCLLHL 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025037.11.1e-222100.00Alcohol dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022937363.11.4e-22299.74alcohol dehydrogenase 1 [Cucurbita moschata][more]
KAG6592176.12.4e-22299.74Alcohol dehydrogenase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023534902.12.4e-22299.47alcohol dehydrogenase 1 [Cucurbita pepo subsp. pepo][more]
XP_022975027.15.0e-22097.63alcohol dehydrogenase 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P128862.5e-19885.98Alcohol dehydrogenase 1 OS=Pisum sativum OX=3888 PE=1 SV=1[more]
P136032.5e-19886.77Alcohol dehydrogenase 1 OS=Trifolium repens OX=3899 GN=ADH1 PE=2 SV=1[more]
P251411.1e-19686.24Alcohol dehydrogenase 1 OS=Petunia hybrida OX=4102 GN=ADH1 PE=1 SV=1[more]
P065256.9e-19684.35Alcohol dehydrogenase class-P OS=Arabidopsis thaliana OX=3702 GN=ADH1 PE=1 SV=2[more]
P489774.5e-19585.45Alcohol dehydrogenase OS=Malus domestica OX=3750 GN=ADH PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FFW16.9e-22399.74alcohol dehydrogenase 1 OS=Cucurbita moschata OX=3662 GN=LOC111443671 PE=3 SV=1[more]
A0A6J1IBW62.4e-22097.63alcohol dehydrogenase 1 OS=Cucurbita maxima OX=3661 GN=LOC111473986 PE=3 SV=1[more]
A0A097BU008.1e-21694.74Alcohol dehydrogenase OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A0A0K6K42.2e-21395.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G322060 PE=3 SV=1[more]
A0A6J1DNQ02.4e-21294.44alcohol dehydrogenase class-P OS=Momordica charantia OX=3673 GN=LOC111021701 PE=... [more]
Match NameE-valueIdentityDescription
AT1G77120.14.9e-19784.35alcohol dehydrogenase 1 [more]
AT5G43940.14.2e-13256.95GroES-like zinc-binding dehydrogenase family protein [more]
AT5G43940.24.4e-12954.92GroES-like zinc-binding dehydrogenase family protein [more]
AT5G24760.17.5e-12152.96GroES-like zinc-binding dehydrogenase family protein [more]
AT1G64710.12.8e-11550.53GroES-like zinc-binding dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013154Alcohol dehydrogenase, N-terminalPFAMPF08240ADH_Ncoord: 36..157
e-value: 3.5E-23
score: 81.6
NoneNo IPR availableGENE3D3.40.50.720coord: 183..321
e-value: 1.5E-147
score: 493.4
NoneNo IPR availableGENE3D3.90.180.10coord: 19..370
e-value: 1.5E-147
score: 493.4
NoneNo IPR availablePANTHERPTHR43880:SF53ALCOHOL DEHYDROGENASE 1coord: 4..378
NoneNo IPR availablePANTHERPTHR43880ALCOHOL DEHYDROGENASEcoord: 4..378
NoneNo IPR availableCDDcd08301alcohol_DH_plantscoord: 9..377
e-value: 0.0
score: 763.375
IPR013149Alcohol dehydrogenase, C-terminalPFAMPF00107ADH_zinc_Ncoord: 206..334
e-value: 1.9E-25
score: 89.4
IPR002328Alcohol dehydrogenase, zinc-type, conserved sitePROSITEPS00059ADH_ZINCcoord: 69..83
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 169..341
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 328..377
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 4..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27258-RACarg27258-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046294 formaldehyde catabolic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004024 alcohol dehydrogenase activity, zinc-dependent
molecular_function GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016491 oxidoreductase activity