Carg21642 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21642
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncytochrome P450 87A3-like
LocationCarg_Chr06: 3793634 .. 3796011 (+)
RNA-Seq ExpressionCarg21642
SyntenyCarg21642
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATGATAAGAGCACATCCAAGCACCACTTACCCACAATACTTCCATTTCAGTTTGCAGCAACCAAAATGTGGGTTCTTTTGATAGGAACTATTGTTGTTTTGAGCTTTACACACTGGCTTTACCGTTGGAGAAACCCCAAATGTAATGGGAAACTCCCACCAGGTTCAATGGGGTTCCCTCTGCTTGGTGAAACCTTCCAGTTCTTCGCTCCCAATACCTCCTCTGATGTCCCTCCTTTCATCAGAAACAGAGTGGATAGGTACGGACCAATCTTCAGAACAAGTTTGGTGGGAAGACCGCTGATTGTCTCAACCGACCCCGACTTCAATCACTTCATCTTTCAACAAGAGGGTCACTTGTTCCAGAGCTGGTATCCATCTACCTTCACCGAGATACTTGGAAGGAAAAATGTTGGTTCTTTGCACGGTTTCATGCATAAGTTTGTGAAGAACATGGTTCTTCATCTTGTTGGCCCTGAAAATCTGAGGAAGATGATATCTGAAGTGGAGGCTGTAGCTACCACAAGATTGAGGCAGTGGTCATCTCTTGAATCGGTTGAATTGAAGGATGAGACTGCAAGTGTAATTACAAAATGAAAAGTTTAGAACCAAACCTGTTTGTTCTCCATGTTTGGTTTGATACTGATTTTTGTTGTTCTTGCAGATGATTTTTAATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTGAAGAGAATTTGAGAGACAACTTTGTTGCCTTCATTCAGGGGTTGATTTCCTTCCCTCTAAATGTTTTTGGAACAGCTTATAACAAATGCTTACAGGTTAGCTAATTATGCTATGCACACTGTGAGTCCTACCTCGATGGGAGAGGAGAATAAACCACACTTCTTAGTAGATGCGTTTTAAAACCGTGAAGCTGACGCCGATACGTAATGGGTCAAAGCAAACAATATCTATTAACGGTGAACTTGAGATGTTATAAATGGTATCAGAGCCAGACATCGGGCGGTGTGCCAACGAAGACGTTGGGCCCCAATGGGGTGGATTGTGAGGTCCCACATCAGTTGGAGAGGGAACTAACCTCTCCTTAATAGACGCATTTTAAAACCGTGAGGCTGACGCCGATACATAACGAGTCAAAGCAGACAATATCTATTAGCGGTGAACTTGAGATGTTACAAATAGTATCAGAGCCAGACATCGGACGGTGTGCTAGCGAGGACGCTGGACTCCAAGGGGTGGATTGTGAGGTCCCACATCGGTTGGAGAGGAAACTAACCTCTTCCTAGTAGATGCGTTTTAAAACTGTGAAGTTGACGGCGATACGTAACGAGCCAAAGTGGACCATATTTGCTAGCGGTGGGCTTGAGCTGTTACAAATGGTATCAGAGCCGAACATCGGGCGGTGTGCTAGCAAAGACGCTGGGCCCCAAGAGGGGTGGATTGTGAGGTCCTACATCCGTTGGAGAGGGGAACAAACCTCTCCTTAATAGACGTGTTTTAAAACCGTGAGATTGATGACGATACATAATAAGCCAAAGCGGACAATACCGACTAGTAGTGGGCTTGAACACCTCCTATAGGAATTGAGGGTCTCTTACCCTAAATCAACAGGGCAGGAAAAGAGCGATGAGGATGCTGAAAAACATGTTGCAAGAAAGGCGTGCAAATCCAAGGAAAGAGCAAACAGATTTTTTTGATTTTGTTCTTGAAGAACTGGCAAAGGATGGAACTCTTCTCACAGAGGAAATTGCTCTGGACCTCATGTTTGTGCTGCTTTTTGCGAGCTTCGAAACAACTTCACTTGCTATTACATTAGCCATTAAATTCCTCCTCAACAATCCTCATGTGCTGGAGGAGCTGACGGTCTGTTTAATGTTGTTTCAACTTTTAACAACAATATCCTCATGTTAAATCCATCATTTGCATCTTTATCAGTGTGAACATGAGGGGATATTGAAGGCAAGGGAGAATCCAGATTGTGGGCTTACATGGGGAGAATACAAATCCATGACATTCACATTTCAGGTTACTTTAATTATTTCATCTCTTTAAGGTCTCTATGTGTCTCATAACTAAACTCCTTCAATTGTCAGTTCATCAATGAAACTGTGAGACTGGCAAATATAGCTCCGGGAATCTTTCGAAAAGCTCTGAGAGACATCCCGTTTAAGGGTATGTATGTATGTATGTATGTATGTATGTATATTGAGCTCAAAGATTCTTCTTCTTCAAAACATCATCACAAATGGCGGCTACACATTTGTTGGAGCAGGATATACCATTCCAGCTGGGTGGTCTGTTATGGTCTGCCCTCCTGCCGTGCACTTGAATCCTGAAATATATGTTGATCCTCTCGCTTTTAATCCATGGCGGTGGAAGGTA

mRNA sequence

GATGATAAGAGCACATCCAAGCACCACTTACCCACAATACTTCCATTTCAGTTTGCAGCAACCAAAATGTGGGTTCTTTTGATAGGAACTATTGTTGTTTTGAGCTTTACACACTGGCTTTACCGTTGGAGAAACCCCAAATGTAATGGGAAACTCCCACCAGGTTCAATGGGGTTCCCTCTGCTTGGTGAAACCTTCCAGTTCTTCGCTCCCAATACCTCCTCTGATGTCCCTCCTTTCATCAGAAACAGAGTGGATAGGTACGGACCAATCTTCAGAACAAGTTTGGTGGGAAGACCGCTGATTGTCTCAACCGACCCCGACTTCAATCACTTCATCTTTCAACAAGAGGGTCACTTGTTCCAGAGCTGGTATCCATCTACCTTCACCGAGATACTTGGAAGGAAAAATGTTGGTTCTTTGCACGGTTTCATGCATAAGTTTGTGAAGAACATGGTTCTTCATCTTGTTGGCCCTGAAAATCTGAGGAAGATGATATCTGAAGTGGAGGCTGTAGCTACCACAAGATTGAGGCAGTGGTCATCTCTTGAATCGGTTGAATTGAAGGATGAGACTGCAAGTATGATTTTTAATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTGAAGAGAATTTGAGAGACAACTTTGTTGCCTTCATTCAGGGGTTGATTTCCTTCCCTCTAAATGTTTTTGGAACAGCTTATAACAAATGCTTACAGGGCAGGAAAAGAGCGATGAGGATGCTGAAAAACATGTTGCAAGAAAGGCGTGCAAATCCAAGGAAAGAGCAAACAGATTTTTTTGATTTTGTTCTTGAAGAACTGGCAAAGGATGGAACTCTTCTCACAGAGGAAATTGCTCTGGACCTCATGTTTGTGCTGCTTTTTGCGAGCTTCGAAACAACTTCACTTGCTATTACATTAGCCATTAAATTCCTCCTCAACAATCCTCATGTGCTGGAGGAGCTGACGTGTGAACATGAGGGGATATTGAAGGCAAGGGAGAATCCAGATTGTGGGCTTACATGGGGAGAATACAAATCCATGACATTCACATTTCAGTTCATCAATGAAACTGTGAGACTGGCAAATATAGCTCCGGGAATCTTTCGAAAAGCTCTGAGAGACATCCCGTTTAAGGGATATACCATTCCAGCTGGGTGGTCTGTTATGGTCTGCCCTCCTGCCGTGCACTTGAATCCTGAAATATATGTTGATCCTCTCGCTTTTAATCCATGGCGGTGGAAGGTA

Coding sequence (CDS)

ATGTGGGTTCTTTTGATAGGAACTATTGTTGTTTTGAGCTTTACACACTGGCTTTACCGTTGGAGAAACCCCAAATGTAATGGGAAACTCCCACCAGGTTCAATGGGGTTCCCTCTGCTTGGTGAAACCTTCCAGTTCTTCGCTCCCAATACCTCCTCTGATGTCCCTCCTTTCATCAGAAACAGAGTGGATAGGTACGGACCAATCTTCAGAACAAGTTTGGTGGGAAGACCGCTGATTGTCTCAACCGACCCCGACTTCAATCACTTCATCTTTCAACAAGAGGGTCACTTGTTCCAGAGCTGGTATCCATCTACCTTCACCGAGATACTTGGAAGGAAAAATGTTGGTTCTTTGCACGGTTTCATGCATAAGTTTGTGAAGAACATGGTTCTTCATCTTGTTGGCCCTGAAAATCTGAGGAAGATGATATCTGAAGTGGAGGCTGTAGCTACCACAAGATTGAGGCAGTGGTCATCTCTTGAATCGGTTGAATTGAAGGATGAGACTGCAAGTATGATTTTTAATCTGACTGCAAAGAAGCTGATTAGTTATGATTCAGAAAATTCTGAAGAGAATTTGAGAGACAACTTTGTTGCCTTCATTCAGGGGTTGATTTCCTTCCCTCTAAATGTTTTTGGAACAGCTTATAACAAATGCTTACAGGGCAGGAAAAGAGCGATGAGGATGCTGAAAAACATGTTGCAAGAAAGGCGTGCAAATCCAAGGAAAGAGCAAACAGATTTTTTTGATTTTGTTCTTGAAGAACTGGCAAAGGATGGAACTCTTCTCACAGAGGAAATTGCTCTGGACCTCATGTTTGTGCTGCTTTTTGCGAGCTTCGAAACAACTTCACTTGCTATTACATTAGCCATTAAATTCCTCCTCAACAATCCTCATGTGCTGGAGGAGCTGACGTGTGAACATGAGGGGATATTGAAGGCAAGGGAGAATCCAGATTGTGGGCTTACATGGGGAGAATACAAATCCATGACATTCACATTTCAGTTCATCAATGAAACTGTGAGACTGGCAAATATAGCTCCGGGAATCTTTCGAAAAGCTCTGAGAGACATCCCGTTTAAGGGATATACCATTCCAGCTGGGTGGTCTGTTATGGTCTGCCCTCCTGCCGTGCACTTGAATCCTGAAATATATGTTGATCCTCTCGCTTTTAATCCATGGCGGTGGAAGGTA

Protein sequence

MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLHGFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAKKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRANPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPHVLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIPFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV
Homology
BLAST of Carg21642 vs. NCBI nr
Match: KAG7028331.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 820.5 bits (2118), Expect = 6.4e-234
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV 400
           FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV 399

BLAST of Carg21642 vs. NCBI nr
Match: KAG6596805.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 816.6 bits (2108), Expect = 9.3e-233
Identity = 397/398 (99.75%), Postives = 397/398 (99.75%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRL QWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 398

BLAST of Carg21642 vs. NCBI nr
Match: XP_022944062.1 (cytochrome P450 87A3-like [Cucurbita moschata])

HSP 1 Score: 815.1 bits (2104), Expect = 2.7e-232
Identity = 396/398 (99.50%), Postives = 397/398 (99.75%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRL QWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFDFVLEELAKDGTLLTEEIALDL+FVLLFASFETTSLAITLAIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLIFVLLFASFETTSLAITLAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 398

BLAST of Carg21642 vs. NCBI nr
Match: KAG7028330.1 (Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 796.6 bits (2056), Expect = 1.0e-226
Identity = 386/399 (96.74%), Postives = 393/399 (98.50%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEI GR+NVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEIFGRQNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFM+KF+KNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQ+RRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQQRRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFD+VLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAIT AIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTW EYKSMTFTFQFINETVRLANI PGIFR+ALRDI 
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWEEYKSMTFTFQFINETVRLANIVPGIFRRALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV 400
           FKGYTIPAGWSVMVCPPAVHLNPE YVDPLAFNPWRW+V
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEV 399

BLAST of Carg21642 vs. NCBI nr
Match: XP_022945165.1 (cytochrome P450 87A3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 795.8 bits (2054), Expect = 1.7e-226
Identity = 386/399 (96.74%), Postives = 392/399 (98.25%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFT+I GR+NVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFM+KF+KNMVLHLVGPENLRKMISEVEAVATTRL QWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFD+VLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAIT AIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTW EYKSMTFTFQFINETVRLANI PGIFRKALRDI 
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV 400
           FKGYTIPAGWSVMVCPPAVHLNPE YVDPLAFNPWRW+V
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEV 399

BLAST of Carg21642 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 519.2 bits (1336), Expect = 4.0e-146
Identity = 242/398 (60.80%), Postives = 312/398 (78.39%), Query Frame = 0

Query: 3   VLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNR 62
           +L      V++   W YRW +P+ NG+LPPGS+G P++GET QFFAPN + D+ PF++ R
Sbjct: 44  LLCAALAAVVALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKER 103

Query: 63  VDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLHGF 122
           + RYG IF+TS+VGRP++VS DP+ N+++FQQEG LF+SWYP TFTEI GR NVGSLHGF
Sbjct: 104 IKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVGSLHGF 163

Query: 123 MHKFVKNMVLHLVGPENLRK-MISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAKK 182
           M+K++K +VL L G ENL+  +++E +A     L  W+S  SVELK+  ++MIF+LTAKK
Sbjct: 164 MYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAKK 223

Query: 183 LISYD-SENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 242
           LI YD S+ S+ NLR NF AFI GLISFPLN+ GTAY++C++GRK AM++L+ M++ER A
Sbjct: 224 LIGYDPSKPSQVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERMA 283

Query: 243 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 302
            P +   DFFD V++EL ++  LLTE IALDLMFVLLFASFETT+LA+T+ +K L  NP 
Sbjct: 284 EPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTENPK 343

Query: 303 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 362
           V++ L  EHE I++ R++P+ G+TW EYKSMTFT Q I E VRLANI PGIFRKAL+D+ 
Sbjct: 344 VVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIVRLANIVPGIFRKALQDVE 403

Query: 363 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
            KGYTIPAGW +MVCPPAVHLNPEIY DPLAFNPWRW+
Sbjct: 404 IKGYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWRWQ 441

BLAST of Carg21642 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.2e-94
Identity = 167/402 (41.54%), Postives = 251/402 (62.44%), Query Frame = 0

Query: 1   MWVLLIG--TIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPF 60
           MW +++G  T+ V  + HW+ +WR+ K NG LPPG+MG PL+GET Q   P+ S DV PF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IRNRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVG- 120
           I+ +V+RYGPIF+T L GRP++VS D +FN++I  QEG   + WY  T ++  G      
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 SLHGFMHKFVKNMVLHLVGPENLR-KMISEVEAVATTRLRQWSSLESVELKDETASMIFN 180
              G +HK+++++ L+  G E LR + +  +EA +   L  WS+  SVE+K+ +A M+F 
Sbjct: 121 KALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFR 180

Query: 181 LTAKKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQ 240
            +  K+   D++    N+   F   + G +S PLN  GT Y+KCL+  K   + L+ ++ 
Sbjct: 181 TSVNKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVVD 240

Query: 241 ERRANPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLL 300
           +R AN   +  DF    L++   +   ++EE  + L+F + FASFE+ S  +TL +K L 
Sbjct: 241 DRLANVGPDVEDFLGQALKDKESE-KFISEEFIIQLLFSISFASFESISTTLTLILKLLD 300

Query: 301 NNPHVLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKAL 360
            +P V++EL  EHE I KAR +PD  +TW EYKSMTFT Q INET+RL ++ P + RK +
Sbjct: 301 EHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRLGSVTPALLRKTV 360

Query: 361 RDIPFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           +D+  KGY IP GW++M+   + H +P++Y DP  FNPWRWK
Sbjct: 361 KDLQVKGYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWK 401

BLAST of Carg21642 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 318.9 bits (816), Expect = 8.0e-86
Identity = 168/401 (41.90%), Postives = 244/401 (60.85%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           +WV+ +  +V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   ++ PF++
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
            R+ +YGP+FRT++ G   +V T+PD    +F+QE   F   YP  F +  G++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMHKFVKNMVLHLVGPENL-RKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTA 180
           G +HK VK + L  +G E L +KMI E++ V    LR  ++  S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFG-TAYNKCLQGRKRAMRMLKNMLQER 240
            K+IS     ++  L DN +A        PL +    +  K    R+ A++++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNN 300
           +A+ R+   DF D ++EE  K+  +  EE A++L+F +L  + E+TS   +LAIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTCEHEGILKARENPDCGLTWGEYK-SMTFTFQFINETVRLANIAPGIFRKALR 360
              L EL  EH  IL+ R     G++W EY+  MTFT   INET+R+AN+AP ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIPFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           D+  KGYTIPAGW V V PPAVH N  IY +PL FNPWRW+
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWE 405

BLAST of Carg21642 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 8.6e-72
Identity = 150/396 (37.88%), Postives = 220/396 (55.56%), Query Frame = 0

Query: 4   LLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNRV 63
           LL+ + +   F   L R R  +    LPPGS+G PL+GETFQ      + +  PFI  RV
Sbjct: 7   LLLLSSIAAGFLLLLRRTRYRRMG--LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 66

Query: 64  DRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLHGFM 123
            RYG +F T L G P I S DP+ N F+ Q EG LF+  YP++   +LG+ ++  + G +
Sbjct: 67  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 126

Query: 124 HKFVKNMVLHLVGPENLR-KMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAKKL 183
           HK + ++ +       ++  ++ +++ +    L  WSS   V L +E   + F LT K+L
Sbjct: 127 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVKQL 186

Query: 184 ISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRANP 243
           +S+D     E+LR  ++  I+G  S PL +F T Y K +Q R++    L  ++ +RR   
Sbjct: 187 MSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEE 246

Query: 244 RKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPHVL 303
            +      D +   LA D     EEI +D +  LL A +ETTS  +TLA+KFL   P  L
Sbjct: 247 EEGAERKKDMLAALLAADDGFSDEEI-VDFLVALLVAGYETTSTIMTLAVKFLTETPLAL 306

Query: 304 EELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIPFK 363
            +L  EHE I +A ++    L W +YKSM FT   +NET+R+ANI  G+FR+A+ D+  K
Sbjct: 307 AQLKEEHEKI-RAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIK 366

Query: 364 GYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           GY IP GW V     AVHL+P  + D   FNPWRW+
Sbjct: 367 GYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQ 396

BLAST of Carg21642 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 7.3e-71
Identity = 161/423 (38.06%), Postives = 235/423 (55.56%), Query Frame = 0

Query: 3   VLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNR 62
           +LL+ +++ L     L + RN K    LPPG  G+P LGET  +  P T++ +  F++  
Sbjct: 12  LLLLPSLLSLLLFLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQH 71

Query: 63  VDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLHGF 122
           V +YG I+R++L G P IVS D   N FI Q EG LF+  YP +   ILG+ ++  L G 
Sbjct: 72  VSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGD 131

Query: 123 MHKFVKNMVLHLVGPENLRK-MISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAKK 182
           MH+ ++++ L+ +    LR  ++ +VE      L  W        +DE     FNL AK 
Sbjct: 132 MHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKH 191

Query: 183 LISYD-SENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 242
           ++S D  E   E L+  +V F++G++S PLN+ GTAY+K LQ R   ++ ++  ++ER+ 
Sbjct: 192 IMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKL 251

Query: 243 ------------------------NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVL 302
                                   + RK++TD  D +L  + K   L TE+I LDL+  L
Sbjct: 252 DIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTD--DDLLGWVLKHSNLSTEQI-LDLILSL 311

Query: 303 LFASFETTSLAITLAIKFLLNNPHVLEELTCEHEGILKA-RENPDCGLTWGEYKSMTFTF 362
           LFA  ET+S+AI LAI FL   P  +EEL  EH  I +A +E  +  L W +YK M FT 
Sbjct: 312 LFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQ 371

Query: 363 QFINETVRLANIAPGIFRKALRDIPFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPW 399
             INET+RL N+   + RKAL+D+ +KGY IP+GW V+    AVHL+   Y  P  FNPW
Sbjct: 372 CVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPW 431

BLAST of Carg21642 vs. ExPASy TrEMBL
Match: A0A6J1FUR3 (cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111448609 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.3e-232
Identity = 396/398 (99.50%), Postives = 397/398 (99.75%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRL QWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFDFVLEELAKDGTLLTEEIALDL+FVLLFASFETTSLAITLAIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLIFVLLFASFETTSLAITLAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 398

BLAST of Carg21642 vs. ExPASy TrEMBL
Match: A0A6J1G055 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449479 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 8.2e-227
Identity = 386/399 (96.74%), Postives = 392/399 (98.25%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFT+I GR+NVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFM+KF+KNMVLHLVGPENLRKMISEVEAVATTRL QWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFD+VLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAIT AIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTW EYKSMTFTFQFINETVRLANI PGIFRKALRDI 
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV 400
           FKGYTIPAGWSVMVCPPAVHLNPE YVDPLAFNPWRW+V
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEV 399

BLAST of Carg21642 vs. ExPASy TrEMBL
Match: A0A6J1G031 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449479 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.4e-226
Identity = 385/398 (96.73%), Postives = 391/398 (98.24%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFT+I GR+NVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTQIFGRQNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFM+KF+KNMVLHLVGPENLRKMISEVEAVATTRL QWSSLESVELKDETASMIFNLTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLTQWSSLESVELKDETASMIFNLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKEQTDFFD+VLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAIT AIKFLLNNPH
Sbjct: 241 NPRKEQTDFFDYVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTW EYKSMTFTFQFINETVRLANI PGIFRKALRDI 
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWDEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           FKGYTIPAGWSVMVCPPAVHLNPE YVDPLAFNPWRW+
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 398

BLAST of Carg21642 vs. ExPASy TrEMBL
Match: A0A6J1KXA8 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498396 PE=4 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 6.7e-221
Identity = 377/399 (94.49%), Postives = 387/399 (96.99%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLL+G IVVLSFTH LYRWRNPKCNGKLPPGSMGFPLLGET QFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLMGAIVVLSFTHCLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEG+LFQSWYPSTFTEI GR+NVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGYLFQSWYPSTFTEIFGRQNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFM+KF+KNMVLHLVGPENLRKMISEVEAVATTRLRQWS LESVELKDETASMIF+LTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLRQWSCLESVELKDETASMIFDLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENS ENLR+NFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSGENLRENFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKE  DFFDFVLEELAKDGTLLTEEIALDLMFVLLF+SFETTS+AIT AIKFLLNNPH
Sbjct: 241 NPRKEHMDFFDFVLEELAKDGTLLTEEIALDLMFVLLFSSFETTSIAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANI PGIFRKALRDI 
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWKV 400
           FKGYTIPAGWSVMVCPPAVHLNPE YVDPLAFNPWRW+V
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWEV 399

BLAST of Carg21642 vs. ExPASy TrEMBL
Match: A0A6J1KZ20 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498396 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.0e-220
Identity = 376/398 (94.47%), Postives = 386/398 (96.98%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           MWVLL+G IVVLSFTH LYRWRNPKCNGKLPPGSMGFPLLGET QFFAPNTSSDVPPFIR
Sbjct: 1   MWVLLMGAIVVLSFTHCLYRWRNPKCNGKLPPGSMGFPLLGETLQFFAPNTSSDVPPFIR 60

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
           NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEG+LFQSWYPSTFTEI GR+NVGSLH
Sbjct: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGYLFQSWYPSTFTEIFGRQNVGSLH 120

Query: 121 GFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAK 180
           GFM+KF+KNMVLHLVGPENLRKMISEVEAVATTRLRQWS LESVELKDETASMIF+LTAK
Sbjct: 121 GFMYKFLKNMVLHLVGPENLRKMISEVEAVATTRLRQWSCLESVELKDETASMIFDLTAK 180

Query: 181 KLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240
           KLISYDSENS ENLR+NFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA
Sbjct: 181 KLISYDSENSGENLRENFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRA 240

Query: 241 NPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPH 300
           NPRKE  DFFDFVLEELAKDGTLLTEEIALDLMFVLLF+SFETTS+AIT AIKFLLNNPH
Sbjct: 241 NPRKEHMDFFDFVLEELAKDGTLLTEEIALDLMFVLLFSSFETTSIAITAAIKFLLNNPH 300

Query: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDIP 360
           VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANI PGIFRKALRDI 
Sbjct: 301 VLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIVPGIFRKALRDIQ 360

Query: 361 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           FKGYTIPAGWSVMVCPPAVHLNPE YVDPLAFNPWRW+
Sbjct: 361 FKGYTIPAGWSVMVCPPAVHLNPEKYVDPLAFNPWRWE 398

BLAST of Carg21642 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 604.4 bits (1557), Expect = 6.8e-173
Identity = 287/399 (71.93%), Postives = 339/399 (84.96%), Query Frame = 0

Query: 1   MWVLLIG-TIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFI 60
           MW LLI  +++++S THW+Y WRNPKC GKLPPGSMGFPLLGE+ QFF PN +SD+PPFI
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  RNRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSL 120
           + RV +YGPIF+T+LVGRP+IVSTD D ++F+F QEG  FQSWYP TFT I G+KNVGSL
Sbjct: 61  KERVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSL 120

Query: 121 HGFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTA 180
           HGFM+K++KNMVL L G + L+KM+ +VE  A  RL  WS+ +SVELKD TASMIF+LTA
Sbjct: 121 HGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTA 180

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERR 240
           KKLIS+D + S ENLR NFVAFIQGLISFP ++ GTAY+KCLQGR +AM+ML+NMLQERR
Sbjct: 181 KKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR 240

Query: 241 ANPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNP 300
            NPRK  +DFFD+V+EE+ K+GT+LTEEIALDLMFVLLFASFETTSLA+TLAIKFL ++P
Sbjct: 241 ENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDP 300

Query: 301 HVLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFRKALRDI 360
            VL+ LT EHE IL+ RE+ D GLTW EYKSMT+TFQFINET RLANI P IFRKALRDI
Sbjct: 301 EVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDI 360

Query: 361 PFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
            FK YTIPAGW+VMVCPPAVHLNPE+Y DPL FNP RW+
Sbjct: 361 KFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWE 399

BLAST of Carg21642 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 599.0 bits (1543), Expect = 2.8e-171
Identity = 288/405 (71.11%), Postives = 339/405 (83.70%), Query Frame = 0

Query: 1   MWVLLIG-TIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFI 60
           MW LLI  +++++S THW+Y WRNPKC GKLPPGSMGFPLLGE+ QFF PN +SD+PPFI
Sbjct: 1   MWALLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFI 60

Query: 61  RNRVD------RYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGR 120
           + RV       RYGPIF+T+LVGRP+IVSTD D ++F+F QEG  FQSWYP TFT I G+
Sbjct: 61  KERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGK 120

Query: 121 KNVGSLHGFMHKFVKNMVLHLVGPENLRKMISEVEAVATTRLRQWSSLESVELKDETASM 180
           KNVGSLHGFM+K++KNMVL L G + L+KM+ +VE  A  RL  WS+ +SVELKD TASM
Sbjct: 121 KNVGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASM 180

Query: 181 IFNLTAKKLISYDSENSEENLRDNFVAFIQGLISFPLNVFGTAYNKCLQGRKRAMRMLKN 240
           IF+LTAKKLIS+D + S ENLR NFVAFIQGLISFP ++ GTAY+KCLQGR +AM+ML+N
Sbjct: 181 IFDLTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRN 240

Query: 241 MLQERRANPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIK 300
           MLQERR NPRK  +DFFD+V+EE+ K+GT+LTEEIALDLMFVLLFASFETTSLA+TLAIK
Sbjct: 241 MLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIK 300

Query: 301 FLLNNPHVLEELTCEHEGILKARENPDCGLTWGEYKSMTFTFQFINETVRLANIAPGIFR 360
           FL ++P VL+ LT EHE IL+ RE+ D GLTW EYKSMT+TFQFINET RLANI P IFR
Sbjct: 301 FLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFR 360

Query: 361 KALRDIPFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           KALRDI FK YTIPAGW+VMVCPPAVHLNPE+Y DPL FNP RW+
Sbjct: 361 KALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWE 405

BLAST of Carg21642 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 330.9 bits (847), Expect = 1.4e-90
Identity = 166/398 (41.71%), Postives = 249/398 (62.56%), Query Frame = 0

Query: 4   LLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIRNRV 63
           +L+  +VV+  +HWLYRW NPKC GKLPPGSMGFP++GET  FF P     +P F++ R+
Sbjct: 9   MLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTFVKKRM 68

Query: 64  DRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLHGFM 123
            RYGP+FRT++ G   +VSTDPD  H IF+QE   F+  YP  F ++ G+ N+     F+
Sbjct: 69  IRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFLKEVFI 128

Query: 124 HKFVKNMVLHLVGPENLRK-MISEVEAVATTRLRQWSSLESVELKDETASMIFNLTAKKL 183
           HK+++ + + ++G E L++ M+  ++      +R  +S  S  ++ E  +++      KL
Sbjct: 129 HKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVAYMTPKL 188

Query: 184 ISYDSENSEENLRDNFVAF-IQGLISFPLNVFGTAYNKCLQGRKRAMRMLKNMLQERRAN 243
           IS     ++  L DN  AF +    SF       A  K L+ R+ A++++K++L  R+  
Sbjct: 189 ISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVLMMRK-E 248

Query: 244 PRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNNPHV 303
            R++Q DF + +LEEL KDG+   +  A++L+F+L FA  E TS    LA+KF+  +P V
Sbjct: 249 TREKQEDFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFISKDPKV 308

Query: 304 LEELTCEHEGILKARENPDCGLTWGEYK-SMTFTFQFINETVRLANIAPGIFRKALRDIP 363
           L EL  EH+ I+  R++ + G++W EY+ +MTFT    NE +RLAN  P +FRKA++D+ 
Sbjct: 309 LAELKREHKAIVDNRKDKEAGVSWEEYRHNMTFTNMVSNEVLRLANTTPLLFRKAVQDVE 368

Query: 364 FKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
            KGYTIPAGW V V P AVH +P IY +P  FNPWRW+
Sbjct: 369 IKGYTIPAGWIVAVAPSAVHFDPAIYENPFEFNPWRWE 405

BLAST of Carg21642 vs. TAIR 10
Match: AT5G48000.2 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 318.9 bits (816), Expect = 5.7e-87
Identity = 168/401 (41.90%), Postives = 244/401 (60.85%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           +WV+ +  +V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   ++ PF++
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
            R+ +YGP+FRT++ G   +V T+PD    +F+QE   F   YP  F +  G++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMHKFVKNMVLHLVGPENL-RKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTA 180
           G +HK VK + L  +G E L +KMI E++ V    LR  ++  S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFG-TAYNKCLQGRKRAMRMLKNMLQER 240
            K+IS     ++  L DN +A        PL +    +  K    R+ A++++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNN 300
           +A+ R+   DF D ++EE  K+  +  EE A++L+F +L  + E+TS   +LAIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTCEHEGILKARENPDCGLTWGEYK-SMTFTFQFINETVRLANIAPGIFRKALR 360
              L EL  EH  IL+ R     G++W EY+  MTFT   INET+R+AN+AP ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIPFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           D+  KGYTIPAGW V V PPAVH N  IY +PL FNPWRW+
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWE 405

BLAST of Carg21642 vs. TAIR 10
Match: AT5G48000.3 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 318.9 bits (816), Expect = 5.7e-87
Identity = 168/401 (41.90%), Postives = 244/401 (60.85%), Query Frame = 0

Query: 1   MWVLLIGTIVVLSFTHWLYRWRNPKCNGKLPPGSMGFPLLGETFQFFAPNTSSDVPPFIR 60
           +WV+ +  +V+   + WLYRW NPKCNGKLPPGSMG P++GET  FF P+   ++ PF++
Sbjct: 9   VWVIAVAAVVI---SKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVK 68

Query: 61  NRVDRYGPIFRTSLVGRPLIVSTDPDFNHFIFQQEGHLFQSWYPSTFTEILGRKNVGSLH 120
            R+ +YGP+FRT++ G   +V T+PD    +F+QE   F   YP  F +  G++NV   H
Sbjct: 69  KRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKH 128

Query: 121 GFMHKFVKNMVLHLVGPENL-RKMISEVEAVATTRLRQWSSLESVELKDETASMIFNLTA 180
           G +HK VK + L  +G E L +KMI E++ V    LR  ++  S + K+   S+I     
Sbjct: 129 GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLT 188

Query: 181 KKLISYDSENSEENLRDNFVAFIQGLISFPLNVFG-TAYNKCLQGRKRAMRMLKNMLQER 240
            K+IS     ++  L DN +A        PL +    +  K    R+ A++++K++   R
Sbjct: 189 PKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRR 248

Query: 241 RANPRKEQTDFFDFVLEELAKDGTLLTEEIALDLMFVLLFASFETTSLAITLAIKFLLNN 300
           +A+ R+   DF D ++EE  K+  +  EE A++L+F +L  + E+TS   +LAIKFL  N
Sbjct: 249 KAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAEN 308

Query: 301 PHVLEELTCEHEGILKARENPDCGLTWGEYK-SMTFTFQFINETVRLANIAPGIFRKALR 360
              L EL  EH  IL+ R     G++W EY+  MTFT   INET+R+AN+AP ++RKA+ 
Sbjct: 309 HKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVN 368

Query: 361 DIPFKGYTIPAGWSVMVCPPAVHLNPEIYVDPLAFNPWRWK 399
           D+  KGYTIPAGW V V PPAVH N  IY +PL FNPWRW+
Sbjct: 369 DVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWE 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028331.16.4e-234100.00Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6596805.19.3e-23399.75Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944062.12.7e-23299.50cytochrome P450 87A3-like [Cucurbita moschata][more]
KAG7028330.11.0e-22696.74Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022945165.11.7e-22696.74cytochrome P450 87A3-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q7XU384.0e-14660.80Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV... [more]
K7NBR21.2e-9441.54Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=... [more]
Q8L7D58.0e-8641.90Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3[more]
Q425698.6e-7237.88Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
O649897.3e-7138.06Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FUR31.3e-23299.50cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111448609 PE=3 SV=... [more]
A0A6J1G0558.2e-22796.74cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114494... [more]
A0A6J1G0312.4e-22696.73cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114494... [more]
A0A6J1KXA86.7e-22194.49cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498396... [more]
A0A6J1KZ202.0e-22094.47cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498396... [more]
Match NameE-valueIdentityDescription
AT1G12740.16.8e-17371.93cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT1G12740.22.8e-17171.11cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT1G78490.11.4e-9041.71cytochrome P450, family 708, subfamily A, polypeptide 3 [more]
AT5G48000.25.7e-8741.90cytochrome P450, family 708, subfamily A, polypeptide 2 [more]
AT5G48000.35.7e-8741.90cytochrome P450, family 708, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 331..347
score: 27.15
coord: 57..80
score: 26.76
coord: 380..398
score: 39.88
coord: 364..378
score: 29.23
IPR001128Cytochrome P450PFAMPF00067p450coord: 31..398
e-value: 2.2E-45
score: 155.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 22..399
e-value: 8.6E-90
score: 303.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..397
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..397
NoneNo IPR availablePANTHERPTHR24286:SF11CYTOCHROME P450, FAMILY 87, SUBFAMILY A, POLYPEPTIDE 2coord: 8..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21642-RACarg21642-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen