Carg05999 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05999
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionLectin_legB domain-containing protein
LocationCarg_Chr04: 21468900 .. 21469277 (-)
RNA-Seq ExpressionCarg05999
SyntenyCarg05999
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGATTTCTCTTCTCCATTGCTTTTCTCTGTTTTCCTCTTCTCATTTCTTCACCCCATTTCTTCATTCAACCGTCGATCCCTCCCCAAACAACCCAATTTCGATCCCCACATTGCCCTCATCGGAGACGCCGTTCTTGTCGACGGCGACGGTCACGGAGACTGCGGCGATTCTTTCGTCAAACTGACCCGCCCTGTCGCTTCCAGCTTTGGCCTTCTCTTGCACAACAAACCCTTGAAGCTTCACGGTTCCACGTCGTTTTATACCCAGTTCACCTTCGCTGTCTCGCCGGACAACGGGGATGGCATCGTTCTTTCGCTTTTTCCCGGTGGGGGTTTTCCCGACGAAATCTCCCCGGAAAAAATGGCTTCATAG

mRNA sequence

ATGGTTGATTTCTCTTCTCCATTGCTTTTCTCTGTTTTCCTCTTCTCATTTCTTCACCCCATTTCTTCATTCAACCGTCGATCCCTCCCCAAACAACCCAATTTCGATCCCCACATTGCCCTCATCGGAGACGCCGTTCTTGTCGACGGCGACGGTCACGGAGACTGCGGCGATTCTTTCGTCAAACTGACCCGCCCTGTCGCTTCCAGCTTTGGCCTTCTCTTGCACAACAAACCCTTGAAGCTTCACGGTTCCACGTCGTTTTATACCCAGTTCACCTTCGCTGTCTCGCCGGACAACGGGGATGGCATCGTTCTTTCGCTTTTTCCCGGTGGGGGTTTTCCCGACGAAATCTCCCCGGAAAAAATGGCTTCATAG

Coding sequence (CDS)

ATGGTTGATTTCTCTTCTCCATTGCTTTTCTCTGTTTTCCTCTTCTCATTTCTTCACCCCATTTCTTCATTCAACCGTCGATCCCTCCCCAAACAACCCAATTTCGATCCCCACATTGCCCTCATCGGAGACGCCGTTCTTGTCGACGGCGACGGTCACGGAGACTGCGGCGATTCTTTCGTCAAACTGACCCGCCCTGTCGCTTCCAGCTTTGGCCTTCTCTTGCACAACAAACCCTTGAAGCTTCACGGTTCCACGTCGTTTTATACCCAGTTCACCTTCGCTGTCTCGCCGGACAACGGGGATGGCATCGTTCTTTCGCTTTTTCCCGGTGGGGGTTTTCCCGACGAAATCTCCCCGGAAAAAATGGCTTCATAG

Protein sequence

MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSFVKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISPEKMAS
Homology
BLAST of Carg05999 vs. NCBI nr
Match: KAG6602634.1 (hypothetical protein SDJN03_07867, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033320.1 hypothetical protein SDJN02_07375, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 259.2 bits (661), Expect = 1.8e-65
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF
Sbjct: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP
Sbjct: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120

Query: 121 EKMAS 126
           EKMAS
Sbjct: 121 EKMAS 125

BLAST of Carg05999 vs. NCBI nr
Match: XP_022961282.1 (uncharacterized protein LOC111461824 [Cucurbita moschata])

HSP 1 Score: 246.9 bits (629), Expect = 9.2e-62
Identity = 120/122 (98.36%), Postives = 120/122 (98.36%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MVDFSSPLLFSVFLFSFLHPISSFN RSLPKQPNFDPHIALIGDAVLVDGDGHGD GDSF
Sbjct: 1   MVDFSSPLLFSVFLFSFLHPISSFNLRSLPKQPNFDPHIALIGDAVLVDGDGHGDFGDSF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP
Sbjct: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120

Query: 121 EK 123
           EK
Sbjct: 121 EK 122

BLAST of Carg05999 vs. NCBI nr
Match: XP_023553507.1 (uncharacterized protein LOC111810901 [Cucurbita pepo subsp. pepo] >XP_023553516.1 uncharacterized protein LOC111810901 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.1 bits (588), Expect = 5.2e-57
Identity = 111/122 (90.98%), Postives = 115/122 (94.26%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MVDFSSP LFSVF FSFLHPISSFN R+LPK+PNFDPHIALIGDAVLVDGDGHGDCGDSF
Sbjct: 1   MVDFSSPFLFSVFFFSFLHPISSFNLRALPKEPNFDPHIALIGDAVLVDGDGHGDCGDSF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRPVASSFGLLLHNKPLKLHGSTSF TQFTFAVSPDNGDG+VLSLF GG FP +ISP
Sbjct: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFNTQFTFAVSPDNGDGLVLSLFRGGDFPHKISP 120

Query: 121 EK 123
           EK
Sbjct: 121 EK 122

BLAST of Carg05999 vs. NCBI nr
Match: XP_008441637.1 (PREDICTED: uncharacterized protein LOC103485717 [Cucumis melo] >KAA0041522.1 L-type lectin-domain containing receptor kinase VIII.1-like [Cucumis melo var. makuwa] >TYK24395.1 L-type lectin-domain containing receptor kinase VIII.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 182.2 bits (461), Expect = 2.8e-42
Identity = 89/122 (72.95%), Postives = 101/122 (82.79%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MV+FSS  LFS+FLF FLHPISSF   + PKQPNFDP ++LIGDA  V  D H + G SF
Sbjct: 1   MVNFSSQFLFSIFLFQFLHPISSF---TFPKQPNFDPQLSLIGDAAFVSTDAHVNGGASF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRP+ASSFGLLLHNKPL +H STSF T FTF+VSP+NGDG+VLSLFPGG FPDE+SP
Sbjct: 61  VKLTRPIASSFGLLLHNKPLTIHESTSFNTHFTFSVSPENGDGLVLSLFPGGIFPDEVSP 119

Query: 121 EK 123
           EK
Sbjct: 121 EK 119

BLAST of Carg05999 vs. NCBI nr
Match: XP_004138574.2 (uncharacterized protein LOC101219233 [Cucumis sativus] >KGN45764.1 hypothetical protein Csa_005767 [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 5.3e-41
Identity = 87/122 (71.31%), Postives = 98/122 (80.33%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MV FSS  L S+FLF FLHPISSF   + PKQPNFDP ++LIGDA  V  D H   G SF
Sbjct: 23  MVHFSSQFLLSIFLFQFLHPISSF---TFPKQPNFDPQLSLIGDAAFVSTDAHLHGGASF 82

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRP+ASSFGLLLHNKPL +H STSF T FTF+VSP+NGDG++LSLFPGG FPDE+SP
Sbjct: 83  VKLTRPIASSFGLLLHNKPLTIHESTSFNTHFTFSVSPENGDGLILSLFPGGVFPDEVSP 141

Query: 121 EK 123
           EK
Sbjct: 143 EK 141

BLAST of Carg05999 vs. ExPASy TrEMBL
Match: A0A6J1H9S2 (uncharacterized protein LOC111461824 OS=Cucurbita moschata OX=3662 GN=LOC111461824 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.5e-62
Identity = 120/122 (98.36%), Postives = 120/122 (98.36%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MVDFSSPLLFSVFLFSFLHPISSFN RSLPKQPNFDPHIALIGDAVLVDGDGHGD GDSF
Sbjct: 1   MVDFSSPLLFSVFLFSFLHPISSFNLRSLPKQPNFDPHIALIGDAVLVDGDGHGDFGDSF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP
Sbjct: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120

Query: 121 EK 123
           EK
Sbjct: 121 EK 122

BLAST of Carg05999 vs. ExPASy TrEMBL
Match: A0A5A7TDM9 (L-type lectin-domain containing receptor kinase VIII.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G001810 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-42
Identity = 89/122 (72.95%), Postives = 101/122 (82.79%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MV+FSS  LFS+FLF FLHPISSF   + PKQPNFDP ++LIGDA  V  D H + G SF
Sbjct: 1   MVNFSSQFLFSIFLFQFLHPISSF---TFPKQPNFDPQLSLIGDAAFVSTDAHVNGGASF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRP+ASSFGLLLHNKPL +H STSF T FTF+VSP+NGDG+VLSLFPGG FPDE+SP
Sbjct: 61  VKLTRPIASSFGLLLHNKPLTIHESTSFNTHFTFSVSPENGDGLVLSLFPGGIFPDEVSP 119

Query: 121 EK 123
           EK
Sbjct: 121 EK 119

BLAST of Carg05999 vs. ExPASy TrEMBL
Match: A0A1S3B3V6 (uncharacterized protein LOC103485717 OS=Cucumis melo OX=3656 GN=LOC103485717 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-42
Identity = 89/122 (72.95%), Postives = 101/122 (82.79%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MV+FSS  LFS+FLF FLHPISSF   + PKQPNFDP ++LIGDA  V  D H + G SF
Sbjct: 1   MVNFSSQFLFSIFLFQFLHPISSF---TFPKQPNFDPQLSLIGDAAFVSTDAHVNGGASF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRP+ASSFGLLLHNKPL +H STSF T FTF+VSP+NGDG+VLSLFPGG FPDE+SP
Sbjct: 61  VKLTRPIASSFGLLLHNKPLTIHESTSFNTHFTFSVSPENGDGLVLSLFPGGIFPDEVSP 119

Query: 121 EK 123
           EK
Sbjct: 121 EK 119

BLAST of Carg05999 vs. ExPASy TrEMBL
Match: A0A0A0KA99 (Lectin_legB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G009430 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.5e-41
Identity = 87/122 (71.31%), Postives = 98/122 (80.33%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MV FSS  L S+FLF FLHPISSF   + PKQPNFDP ++LIGDA  V  D H   G SF
Sbjct: 23  MVHFSSQFLLSIFLFQFLHPISSF---TFPKQPNFDPQLSLIGDAAFVSTDAHLHGGASF 82

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRP+ASSFGLLLHNKPL +H STSF T FTF+VSP+NGDG++LSLFPGG FPDE+SP
Sbjct: 83  VKLTRPIASSFGLLLHNKPLTIHESTSFNTHFTFSVSPENGDGLILSLFPGGVFPDEVSP 141

Query: 121 EK 123
           EK
Sbjct: 143 EK 141

BLAST of Carg05999 vs. ExPASy TrEMBL
Match: A0A6J1BW10 (uncharacterized protein LOC111006026 OS=Momordica charantia OX=3673 GN=LOC111006026 PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.3e-32
Identity = 77/122 (63.11%), Postives = 90/122 (73.77%), Query Frame = 0

Query: 1   MVDFSSPLLFSVFLFSFLHPISSFNRRSLPKQPNFDPHIALIGDAVLVDGDGHGDCGDSF 60
           MV+  S LL  +F + FLHP+SSF   +L   PNFDPH+ALIGDA +V  D   D  DSF
Sbjct: 1   MVNLPSHLLIFLFFYYFLHPVSSFTLPTLSNNPNFDPHVALIGDAAVVHAD--ADADDSF 60

Query: 61  VKLTRPVASSFGLLLHNKPLKLHGSTSFYTQFTFAVSPDNGDGIVLSLFPGGGFPDEISP 120
           VKLTRPVASSFGL+L+ KPL+LH S+SF TQF F VSPDNGDG+VLSLFPG     E+S 
Sbjct: 61  VKLTRPVASSFGLVLNAKPLRLHDSSSFSTQFAFWVSPDNGDGLVLSLFPGS---FELSR 117

Query: 121 EK 123
           EK
Sbjct: 121 EK 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6602634.11.8e-65100.00hypothetical protein SDJN03_07867, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022961282.19.2e-6298.36uncharacterized protein LOC111461824 [Cucurbita moschata][more]
XP_023553507.15.2e-5790.98uncharacterized protein LOC111810901 [Cucurbita pepo subsp. pepo] >XP_023553516.... [more]
XP_008441637.12.8e-4272.95PREDICTED: uncharacterized protein LOC103485717 [Cucumis melo] >KAA0041522.1 L-t... [more]
XP_004138574.25.3e-4171.31uncharacterized protein LOC101219233 [Cucumis sativus] >KGN45764.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1H9S24.5e-6298.36uncharacterized protein LOC111461824 OS=Cucurbita moschata OX=3662 GN=LOC1114618... [more]
A0A5A7TDM91.3e-4272.95L-type lectin-domain containing receptor kinase VIII.1-like OS=Cucumis melo var.... [more]
A0A1S3B3V61.3e-4272.95uncharacterized protein LOC103485717 OS=Cucumis melo OX=3656 GN=LOC103485717 PE=... [more]
A0A0A0KA992.5e-4171.31Lectin_legB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G009430... [more]
A0A6J1BW106.3e-3263.11uncharacterized protein LOC111006026 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.120.200coord: 23..122
e-value: 6.5E-7
score: 31.3
NoneNo IPR availablePANTHERPTHR32401CONCANAVALIN A-LIKE LECTIN FAMILY PROTEINcoord: 9..116
NoneNo IPR availablePANTHERPTHR32401:SF15LEGUME LECTIN BETA DOMAIN PROTEINcoord: 9..116
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 35..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05999-RACarg05999-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030246 carbohydrate binding