Cp4.1LG17g10020 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG17g10020 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 6.2e-138
Identity = 274/616 (44.48%), Postives = 365/616 (59.25%), Query Frame = 0

Query: 44  ALECLGRGFDLTNDFRLKFVK-GSG-AGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRC 103
           A++ LG+GFDLT DFRLK+ K G G AG  RLVV+D    R++  P  GV Q VS DI C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 104 DKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNL-SGDWFHDAADANYLAF 163
           DKG+R RF+SD+L FN+MSE  NQRSS+ GK+PSG FN+ F   SG W  DAA+   L  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 164 DGYFIALYYLHL-TASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLI 223
           D   + L+ LH+   + L L D V+ AVPS+WDP  L+RFI+ YGTH+I G ++GGQD++
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 224 SVRQRPSSSIPPAELRRHLEDLGDILF------SDKRSPSSLHRK--------------- 283
            VRQ  SS +    LR HL DLGD LF      S +R   + H                 
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 284 -----------RDVKGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLTG 343
                          GIT+IC+KRGGD    SH+ WL TV  KP+ I F F+PITSLL  
Sbjct: 252 KQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKD 311

Query: 344 VAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATC-PTLQF 403
           V GSG LSHA++LYLRYKP L DLQYFL+F  P  WAP   +LP G     A+  P L  
Sbjct: 312 VPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHI 371

Query: 404 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 463
            FM P+ Y+ +T VTS + P+ G+R +LEGKK N LA+H+QHL +    +   I     +
Sbjct: 372 NFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI-----T 431

Query: 464 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNG------VYIVTGA 523
               W G+D    N R+ EP+  K++S+VCT  V++DPNW+K   N        +IVTGA
Sbjct: 432 DEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGA 491

Query: 524 QLISKGKWPKTILHLRLQFTHLPNCTIRRTEWAAAPEG-SRKSTFLTNLSTTFT----FT 583
           QL  K    K++LHLRL++T + +  + +  W   P G S+KS   +++S   T      
Sbjct: 492 QLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHH 551

Query: 584 QRAAADKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKL 612
                DK + V   L+SGV+   PPVP  + K++K+VD +++ +GP  +PGHWLVT  +L
Sbjct: 552 NMIQKDKNEVV---LDSGVFPGGPPVPA-NNKIVKFVDLSQLCRGPQHSPGHWLVTGVRL 611

BLAST of Cp4.1LG17g10020 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 2.4e-134
Identity = 257/599 (42.90%), Postives = 365/599 (60.93%), Query Frame = 0

Query: 44  ALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRCDK 103
           A+  +G G+DL  D RLK+ KG     R L + +  +  +IV P G  +  VS+ I+CDK
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 104 GDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDAADANYLAFDGY 163
           G+R+RF+SD+L F QM+E  NQ  S+ GK+PSG FN+MF  S  W  DAA    LAFDG 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 164 FIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLISVRQ 223
           FI+LY + L  S ++L++ VK+AVPS WDPA+L+RFI  YGTHIIV   +GG+D+I  +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 224 RPSSSIPPAELRRHLEDLGD-----------------------------ILFSDKRSPSS 283
           + SS + P +L++ L+++ D                             + F+D  S  S
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 284 LHRKRDVKGITLICSKRGGDPFLH-SHTSWLQTVSFKPEVILFKFVPITSLLTGVAGSGY 343
              K D      +C +RGG+   +  H  WLQTV  +P+VI   F+PITSLL GV GSG+
Sbjct: 254 YANKED---YVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGF 313

Query: 344 LSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQFTFMSPRT 403
           LSHAINLYLRYKP + +L  FLEFQ+P QWAP F ELPLG QR++ +C +LQF+F  P+ 
Sbjct: 314 LSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKL 373

Query: 404 YITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNESRRSQWIG 463
           Y+ +T V   ++P+ G+RLYLEG++SN LA+H+QHL+SLPK+  +L    N S R +   
Sbjct: 374 YVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIY-QLEDDLNRSIRQE--- 433

Query: 464 TDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKGKWPKTIL 523
                 + R+ E V WK YS+VCT  V  D        + + +VTGAQL  +    K +L
Sbjct: 434 ----SHDRRYYEKVNWKNYSHVCTEPVESD--------DDLSVVTGAQLHVESHGFKNVL 493

Query: 524 HLRLQFTHLPNCT-IRRTEWAAAPEGSRKSTFLTNL-STTFTFTQRAAADKKKQVPGALN 583
            LRL F+ +   T ++ +EW  A   + KS  ++ L S  FT  Q+      +     +N
Sbjct: 494 FLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQK---PPPRPADVNIN 553

Query: 584 SGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNVKFAL 611
           S +Y   PPVP ++ KLLK+VDT+E+ +GP ++PG+W+V+ A+L+ E GKI L VK++L
Sbjct: 554 SAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Cp4.1LG17g10020 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 6.0e-125
Identity = 251/602 (41.69%), Postives = 353/602 (58.64%), Query Frame = 0

Query: 43  RALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRCD 102
           +A+  +G G+DL +D R    K +   G RLV ID    RD++FP G VV  VS  I+CD
Sbjct: 17  KAVSVIGLGYDLCSDVRFSACK-TTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 76

Query: 103 KGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDAADANYLAFDG 162
           KG+R R +SD+L FNQMSE  NQ   + GK+PSG FN+MF  S  W  DA+    LA+DG
Sbjct: 77  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDG 136

Query: 163 YFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLISVR 222
           +FI+LY + +    L L+DEVK+ VPS+WD A+L+ FI+ YGTH++VG  +GG+D+I V+
Sbjct: 137 WFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVK 196

Query: 223 QRPSSSIPPAELRRHLEDLGDILFS----DKRSPSS------------------------ 282
           Q   S+  P E+++ L+  GD  F     + +SP+S                        
Sbjct: 197 QMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVS 256

Query: 283 ----LHRKRDVKGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLTGVAG 342
               +H K +   I  +C +RGG     SH  WL TVS  P VI   FVPITSLL+G+ G
Sbjct: 257 SAVVMHTKNE--EIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 316

Query: 343 SGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQFTFMS 402
           +G+LSHA+NLYLRYKP + +L  FLEFQ+P QWAP + +LPLG +R K + P+LQF+ M 
Sbjct: 317 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 376

Query: 403 PRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNESRRSQ 462
           P+ Y+ +++V S  +P+ GLR +LEGKK N LA+H+QHL++ P  +       + S    
Sbjct: 377 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL-------HLSHDDT 436

Query: 463 WIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKGKWPK 522
           +   ++    G ++ PVKW  +S+VCT  V++  N  + D +   IVT A L  KG   +
Sbjct: 437 YEPIEEPVEKGYYV-PVKWGIFSHVCTYPVQY--NGARSD-DTASIVTKAWLEVKGMGMR 496

Query: 523 TILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTF-TFTQRAAADKKKQVPGA 582
            +L LRL F+   +   R++ W      SRKS   + +ST   T      A  K Q    
Sbjct: 497 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID 556

Query: 583 LNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNVKFA 612
           +NS VY   P  PV+  KLL  VDT EV++GP + PG+W+VT AKL  E GKI +  K++
Sbjct: 557 INSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYS 603

BLAST of Cp4.1LG17g10020 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 7.9e-125
Identity = 246/574 (42.86%), Postives = 340/574 (59.23%), Query Frame = 0

Query: 44  ALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRCDK 103
           A++ LGRGFD+T+D RL + K  GA G RLV I++   RD+    G ++  V  DI C +
Sbjct: 24  AIQALGRGFDVTSDVRLLYCK--GAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSR 83

Query: 104 GDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDAADANYLAFDGY 163
           G+    +  V  F++M+E  N RS ++G +P G FN+MFN +G W  DAA    LA  GY
Sbjct: 84  GNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGY 143

Query: 164 FIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLISVRQ 223
           FI LY + L    LVL +E+++AVPS+WDPASL+ FI+ YGTHI+    IGG+D++ +RQ
Sbjct: 144 FIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQ 203

Query: 224 RPSSSIPPAELRRHLEDLGDILFSDKRSPS-SLHRKRDVKGITLICSKRGGDPFLHSHTS 283
             SS +P +E+  ++ D+    F +  S S +   K   K IT+I  +RGGD    SH  
Sbjct: 204 HQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDDLEQSHAR 263

Query: 284 WLQTVSFKPEVILFKFVPITSLLTGVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQ 343
           W +TV   P++I   F PI SLL GV G  +L+ AI LYL YKP + DLQYFL++QI   
Sbjct: 264 WAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARA 323

Query: 344 WAPNFCELPLGHQRRKATCPTLQFTFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWL 403
           WAP    L    QR++  C +LQF+ M P+ +I++ QVT  RKP+ GLRL LEG K N L
Sbjct: 324 WAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRL 383

Query: 404 ALHVQHLTSLPKVMARLIGTSNESRRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRH 463
           ++H+QHL SLPK++     +       +W G ++ DS  R+ EP+KWK +S+V TS + H
Sbjct: 384 SIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDS--RWFEPIKWKNFSHVSTSPIEH 443

Query: 464 DPNWLKQDGNGVYIVTGAQLISKGKW---PKTILHLRLQFTHLPNCTIRRTEWAAAPEGS 523
               +  D +GV+IVTGAQL   G W    K +LHL+L F+ +P CTIRR+ W   P   
Sbjct: 444 TETHI-GDLSGVHIVTGAQL---GVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTP--- 503

Query: 524 RKSTFLTNLSTTFTFTQRAAADKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVK 583
                               A   +  PG  ++        V  +S KL K VD++E++K
Sbjct: 504 -------------------VASSGRLEPGGPSTS--SSTEEVSGQSGKLAKIVDSSEMLK 561

Query: 584 GPHDTPGHWLVTAAKLVTEGGKIGLNVKFALLDY 614
           GP D PGHWLVT AKL  E GKI L VK++LL+Y
Sbjct: 564 GPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Cp4.1LG17g10020 vs. NCBI nr
Match: XP_023514305.1 (MACPF domain-containing protein At1g14780-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1169 bits (3024), Expect = 0.0
Identity = 582/608 (95.72%), Postives = 582/608 (95.72%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK          
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
           RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG
Sbjct: 421 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 614
           PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. NCBI nr
Match: KAG7025290.1 (MACPF domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1163 bits (3009), Expect = 0.0
Identity = 577/608 (94.90%), Postives = 582/608 (95.72%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK          
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTS++S
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDQS 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
           RRSQW+GTDDSDSNGRFL+PVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG
Sbjct: 421 RRSQWVGTDDSDSNGRFLDPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 614
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. NCBI nr
Match: KAG6592885.1 (MACPF domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1162 bits (3007), Expect = 0.0
Identity = 577/608 (94.90%), Postives = 582/608 (95.72%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMF+LSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFSLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK          
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTS++S
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDQS 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
           RRSQW+GTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG
Sbjct: 421 RRSQWVGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 614
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. NCBI nr
Match: XP_022960284.1 (MACPF domain-containing protein At1g14780-like [Cucurbita moschata])

HSP 1 Score: 1162 bits (3007), Expect = 0.0
Identity = 577/608 (94.90%), Postives = 582/608 (95.72%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPS+WDPASLSRFIQTYGTHIIVGTA
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSHWDPASLSRFIQTYGTHIIVGTA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK          
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTS++S
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDQS 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
           RRSQW+GTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG
Sbjct: 421 RRSQWVGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 614
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. NCBI nr
Match: XP_023004966.1 (MACPF domain-containing protein At1g14780-like [Cucurbita maxima])

HSP 1 Score: 1159 bits (2998), Expect = 0.0
Identity = 578/608 (95.07%), Postives = 580/608 (95.39%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIAL YLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA
Sbjct: 121 ADANYLAFDGYFIALCYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK          
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTS+ES
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDES 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
           RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQD NGVYIVTGAQLISKG
Sbjct: 421 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDVNGVYIVTGAQLISKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 614
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. ExPASy TrEMBL
Match: A0A6J1HAI4 (MACPF domain-containing protein At1g14780-like OS=Cucurbita moschata OX=3662 GN=LOC111461072 PE=4 SV=1)

HSP 1 Score: 1162 bits (3007), Expect = 0.0
Identity = 577/608 (94.90%), Postives = 582/608 (95.72%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPS+WDPASLSRFIQTYGTHIIVGTA
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSHWDPASLSRFIQTYGTHIIVGTA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK          
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTS++S
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDQS 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
           RRSQW+GTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG
Sbjct: 421 RRSQWVGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 614
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. ExPASy TrEMBL
Match: A0A6J1KTM0 (MACPF domain-containing protein At1g14780-like OS=Cucurbita maxima OX=3661 GN=LOC111498109 PE=4 SV=1)

HSP 1 Score: 1159 bits (2998), Expect = 0.0
Identity = 578/608 (95.07%), Postives = 580/608 (95.39%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV
Sbjct: 1   MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA
Sbjct: 61  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIAL YLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA
Sbjct: 121 ADANYLAFDGYFIALCYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK          
Sbjct: 181 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVKVPEIFTKILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLANISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTS+ES
Sbjct: 361 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSDES 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
           RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQD NGVYIVTGAQLISKG
Sbjct: 421 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDVNGVYIVTGAQLISKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV
Sbjct: 481 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 614
           PGALNSGVYGEAPPVPVRSKKLLKYVDT+EVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTSEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. ExPASy TrEMBL
Match: A0A0A0KDR9 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G022370 PE=4 SV=1)

HSP 1 Score: 1086 bits (2808), Expect = 0.0
Identity = 534/617 (86.55%), Postives = 564/617 (91.41%), Query Frame = 0

Query: 23  WSNLGAGGSTMISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKR 82
           + +L  GGS M+S EK VE RAL+CLGRGFDLTNDFRLKFVKG+G GGRRLVV+DDVNKR
Sbjct: 58  FDDLAVGGSAMLSPEKSVEVRALDCLGRGFDLTNDFRLKFVKGNGGGGRRLVVVDDVNKR 117

Query: 83  DIVFPDGGVVQGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMF 142
           D+VFPDG VV+GVSQDIRCDKGDRIRFKSDVLQFNQMSE LNQ+SSIQGKVPSGYFNSMF
Sbjct: 118 DMVFPDGAVVRGVSQDIRCDKGDRIRFKSDVLQFNQMSETLNQKSSIQGKVPSGYFNSMF 177

Query: 143 NLSGDWFHDAADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQT 202
           +LSGDWFHDAADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASL+RFIQT
Sbjct: 178 DLSGDWFHDAADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLARFIQT 237

Query: 203 YGTHIIVGTAIGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK 262
           YGTHIIVGTAIGGQDLISVRQRPSS IPPAE+RRHLEDLGDILFSDKRSPS+L R+RD K
Sbjct: 238 YGTHIIVGTAIGGQDLISVRQRPSSPIPPAEVRRHLEDLGDILFSDKRSPSTLQRRRDGK 297

Query: 263 --------------------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILF 322
                                     GITLICSKRGGDPFLHSHTSWLQTVS KPEVILF
Sbjct: 298 VPEIFTRILQSSSIQLTSISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILF 357

Query: 323 KFVPITSLLTGVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQR 382
           KFVPITSLLTGVAGSGYLSHAINLYLRYKPSL DLQYFLEFQIPTQWAPNFCELPLGHQR
Sbjct: 358 KFVPITSLLTGVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQR 417

Query: 383 RKATCPTLQFTFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVM 442
           +KA+CPTLQFTFMSPRTY+TSTQVTSS KPLVGLRLYLEGK+SNWLALHVQHLTSLPKVM
Sbjct: 418 KKASCPTLQFTFMSPRTYVTSTQVTSSTKPLVGLRLYLEGKRSNWLALHVQHLTSLPKVM 477

Query: 443 ARLIGTSNESRRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYI 502
           ARL+G+S+ S RS+W+G+DDS SN +FLE VKWK YSNVCTSVVRHDPNWL+QDG+GVYI
Sbjct: 478 ARLMGSSDVSSRSRWLGSDDSHSNSQFLEAVKWKSYSNVCTSVVRHDPNWLQQDGDGVYI 537

Query: 503 VTGAQLISKGKWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQ 562
           VTGAQL++KGKWPKTILHLRLQFTHLPNC IRRTEWAAAPE  RKSTFLTNLSTTFTFTQ
Sbjct: 538 VTGAQLLTKGKWPKTILHLRLQFTHLPNCAIRRTEWAAAPETGRKSTFLTNLSTTFTFTQ 597

Query: 563 RAAADKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLV 613
           RAA DKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVV+GPHD PGHWLVTAAKLV
Sbjct: 598 RAAGDKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVRGPHDAPGHWLVTAAKLV 657

BLAST of Cp4.1LG17g10020 vs. ExPASy TrEMBL
Match: A0A5D3BTB4 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold69G00060 PE=4 SV=1)

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 532/607 (87.64%), Postives = 557/607 (91.76%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           M+S EK VE RAL+CLGRGFDLTNDFRLKFVKG+GAGGRRLVV+DDVNKRD+VFPDG VV
Sbjct: 1   MLSPEKSVEVRALDCLGRGFDLTNDFRLKFVKGNGAGGRRLVVVDDVNKRDMVFPDGAVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           +GVSQDIRCDKGDRIRFKSDVLQFNQMSE LNQ+SSIQGKVPSGYFNSMF+LSGDWFHDA
Sbjct: 61  RGVSQDIRCDKGDRIRFKSDVLQFNQMSETLNQKSSIQGKVPSGYFNSMFDLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASL+RFIQTYGTHIIVG A
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLARFIQTYGTHIIVGMA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSS IPPAELRRHLEDLGDILFSDKRSPS+L RKRD K          
Sbjct: 181 IGGQDLISVRQRPSSPIPPAELRRHLEDLGDILFSDKRSPSTLQRKRDGKVPEIFTRILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVS KPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLTSISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSL DLQYFLEFQIPTQWAPNFCELPLGHQR+KA+CPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKASCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTY+TSTQVTSS KPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARL+GTS+  
Sbjct: 361 TFMSPRTYVTSTQVTSSTKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLMGTSDVL 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
            RS+W+G+DDSDS+ +FLE VKWK YSNVCTSVVRHD NWL+QDG+GVYIVTGAQLI+KG
Sbjct: 421 SRSRWLGSDDSDSSSQFLEAVKWKSYSNVCTSVVRHDSNWLQQDGDGVYIVTGAQLITKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQF+HLPNC IRRTEWAAAPE  RKSTFLTNLSTTFTFTQRAA DKKKQV
Sbjct: 481 KWPKTILHLRLQFSHLPNCAIRRTEWAAAPETGRKSTFLTNLSTTFTFTQRAAGDKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 613
           PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVV+GPHD PGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVRGPHDAPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. ExPASy TrEMBL
Match: A0A1S3BD48 (MACPF domain-containing protein At4g24290-like OS=Cucumis melo OX=3656 GN=LOC103488584 PE=4 SV=1)

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 532/607 (87.64%), Postives = 557/607 (91.76%), Query Frame = 0

Query: 33  MISTEKPVEARALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVV 92
           M+S EK VE RAL+CLGRGFDLTNDFRLKFVKG+GAGGRRLVV+DDVNKRD+VFPDG VV
Sbjct: 1   MLSPEKSVEVRALDCLGRGFDLTNDFRLKFVKGNGAGGRRLVVVDDVNKRDMVFPDGAVV 60

Query: 93  QGVSQDIRCDKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDA 152
           +GVSQDIRCDKGDRIRFKSDVLQFNQMSE LNQ+SSIQGKVPSGYFNSMF+LSGDWFHDA
Sbjct: 61  RGVSQDIRCDKGDRIRFKSDVLQFNQMSETLNQKSSIQGKVPSGYFNSMFDLSGDWFHDA 120

Query: 153 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTA 212
           ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASL+RFIQTYGTHIIVG A
Sbjct: 121 ADANYLAFDGYFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLARFIQTYGTHIIVGMA 180

Query: 213 IGGQDLISVRQRPSSSIPPAELRRHLEDLGDILFSDKRSPSSLHRKRDVK---------- 272
           IGGQDLISVRQRPSS IPPAELRRHLEDLGDILFSDKRSPS+L RKRD K          
Sbjct: 181 IGGQDLISVRQRPSSPIPPAELRRHLEDLGDILFSDKRSPSTLQRKRDGKVPEIFTRILQ 240

Query: 273 ----------------GITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLT 332
                           GITLICSKRGGDPFLHSHTSWLQTVS KPEVILFKFVPITSLLT
Sbjct: 241 SSSIQLTSISETSSKDGITLICSKRGGDPFLHSHTSWLQTVSSKPEVILFKFVPITSLLT 300

Query: 333 GVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQF 392
           GVAGSGYLSHAINLYLRYKPSL DLQYFLEFQIPTQWAPNFCELPLGHQR+KA+CPTLQF
Sbjct: 301 GVAGSGYLSHAINLYLRYKPSLGDLQYFLEFQIPTQWAPNFCELPLGHQRKKASCPTLQF 360

Query: 393 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 452
           TFMSPRTY+TSTQVTSS KPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARL+GTS+  
Sbjct: 361 TFMSPRTYVTSTQVTSSTKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLMGTSDVL 420

Query: 453 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKG 512
            RS+W+G+DDSDS+ +FLE VKWK YSNVCTSVVRHD NWL+QDG+GVYIVTGAQLI+KG
Sbjct: 421 SRSRWLGSDDSDSSSQFLEAVKWKSYSNVCTSVVRHDSNWLQQDGDGVYIVTGAQLITKG 480

Query: 513 KWPKTILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTFTFTQRAAADKKKQV 572
           KWPKTILHLRLQF+HLPNC IRRTEWAAAPE  RKSTFLTNLSTTFTFTQRAA DKKKQV
Sbjct: 481 KWPKTILHLRLQFSHLPNCAIRRTEWAAAPETGRKSTFLTNLSTTFTFTQRAAGDKKKQV 540

Query: 573 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNV 613
           PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVV+GPHD PGHWLVTAAKLVTEGGKIGLNV
Sbjct: 541 PGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVRGPHDAPGHWLVTAAKLVTEGGKIGLNV 600

BLAST of Cp4.1LG17g10020 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 492.7 bits (1267), Expect = 4.4e-139
Identity = 274/616 (44.48%), Postives = 365/616 (59.25%), Query Frame = 0

Query: 44  ALECLGRGFDLTNDFRLKFVK-GSG-AGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRC 103
           A++ LG+GFDLT DFRLK+ K G G AG  RLVV+D    R++  P  GV Q VS DI C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 104 DKGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNL-SGDWFHDAADANYLAF 163
           DKG+R RF+SD+L FN+MSE  NQRSS+ GK+PSG FN+ F   SG W  DAA+   L  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 164 DGYFIALYYLHL-TASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLI 223
           D   + L+ LH+   + L L D V+ AVPS+WDP  L+RFI+ YGTH+I G ++GGQD++
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 224 SVRQRPSSSIPPAELRRHLEDLGDILF------SDKRSPSSLHRK--------------- 283
            VRQ  SS +    LR HL DLGD LF      S +R   + H                 
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 284 -----------RDVKGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLTG 343
                          GIT+IC+KRGGD    SH+ WL TV  KP+ I F F+PITSLL  
Sbjct: 252 KQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKD 311

Query: 344 VAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATC-PTLQF 403
           V GSG LSHA++LYLRYKP L DLQYFL+F  P  WAP   +LP G     A+  P L  
Sbjct: 312 VPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHI 371

Query: 404 TFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNES 463
            FM P+ Y+ +T VTS + P+ G+R +LEGKK N LA+H+QHL +    +   I     +
Sbjct: 372 NFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI-----T 431

Query: 464 RRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNG------VYIVTGA 523
               W G+D    N R+ EP+  K++S+VCT  V++DPNW+K   N        +IVTGA
Sbjct: 432 DEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGA 491

Query: 524 QLISKGKWPKTILHLRLQFTHLPNCTIRRTEWAAAPEG-SRKSTFLTNLSTTFT----FT 583
           QL  K    K++LHLRL++T + +  + +  W   P G S+KS   +++S   T      
Sbjct: 492 QLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHH 551

Query: 584 QRAAADKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKL 612
                DK + V   L+SGV+   PPVP  + K++K+VD +++ +GP  +PGHWLVT  +L
Sbjct: 552 NMIQKDKNEVV---LDSGVFPGGPPVPA-NNKIVKFVDLSQLCRGPQHSPGHWLVTGVRL 611

BLAST of Cp4.1LG17g10020 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.7e-135
Identity = 257/599 (42.90%), Postives = 365/599 (60.93%), Query Frame = 0

Query: 44  ALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRCDK 103
           A+  +G G+DL  D RLK+ KG     R L + +  +  +IV P G  +  VS+ I+CDK
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 104 GDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDAADANYLAFDGY 163
           G+R+RF+SD+L F QM+E  NQ  S+ GK+PSG FN+MF  S  W  DAA    LAFDG 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 164 FIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLISVRQ 223
           FI+LY + L  S ++L++ VK+AVPS WDPA+L+RFI  YGTHIIV   +GG+D+I  +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 224 RPSSSIPPAELRRHLEDLGD-----------------------------ILFSDKRSPSS 283
           + SS + P +L++ L+++ D                             + F+D  S  S
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 284 LHRKRDVKGITLICSKRGGDPFLH-SHTSWLQTVSFKPEVILFKFVPITSLLTGVAGSGY 343
              K D      +C +RGG+   +  H  WLQTV  +P+VI   F+PITSLL GV GSG+
Sbjct: 254 YANKED---YVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGF 313

Query: 344 LSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQFTFMSPRT 403
           LSHAINLYLRYKP + +L  FLEFQ+P QWAP F ELPLG QR++ +C +LQF+F  P+ 
Sbjct: 314 LSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKL 373

Query: 404 YITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNESRRSQWIG 463
           Y+ +T V   ++P+ G+RLYLEG++SN LA+H+QHL+SLPK+  +L    N S R +   
Sbjct: 374 YVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIY-QLEDDLNRSIRQE--- 433

Query: 464 TDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKGKWPKTIL 523
                 + R+ E V WK YS+VCT  V  D        + + +VTGAQL  +    K +L
Sbjct: 434 ----SHDRRYYEKVNWKNYSHVCTEPVESD--------DDLSVVTGAQLHVESHGFKNVL 493

Query: 524 HLRLQFTHLPNCT-IRRTEWAAAPEGSRKSTFLTNL-STTFTFTQRAAADKKKQVPGALN 583
            LRL F+ +   T ++ +EW  A   + KS  ++ L S  FT  Q+      +     +N
Sbjct: 494 FLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQK---PPPRPADVNIN 553

Query: 584 SGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNVKFAL 611
           S +Y   PPVP ++ KLLK+VDT+E+ +GP ++PG+W+V+ A+L+ E GKI L VK++L
Sbjct: 554 SAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Cp4.1LG17g10020 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 449.5 bits (1155), Expect = 4.3e-126
Identity = 251/602 (41.69%), Postives = 353/602 (58.64%), Query Frame = 0

Query: 43  RALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRCD 102
           +A+  +G G+DL +D R    K +   G RLV ID    RD++FP G VV  VS  I+CD
Sbjct: 17  KAVSVIGLGYDLCSDVRFSACK-TTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 76

Query: 103 KGDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDAADANYLAFDG 162
           KG+R R +SD+L FNQMSE  NQ   + GK+PSG FN+MF  S  W  DA+    LA+DG
Sbjct: 77  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDG 136

Query: 163 YFIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLISVR 222
           +FI+LY + +    L L+DEVK+ VPS+WD A+L+ FI+ YGTH++VG  +GG+D+I V+
Sbjct: 137 WFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVK 196

Query: 223 QRPSSSIPPAELRRHLEDLGDILFS----DKRSPSS------------------------ 282
           Q   S+  P E+++ L+  GD  F     + +SP+S                        
Sbjct: 197 QMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVS 256

Query: 283 ----LHRKRDVKGITLICSKRGGDPFLHSHTSWLQTVSFKPEVILFKFVPITSLLTGVAG 342
               +H K +   I  +C +RGG     SH  WL TVS  P VI   FVPITSLL+G+ G
Sbjct: 257 SAVVMHTKNE--EIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 316

Query: 343 SGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQWAPNFCELPLGHQRRKATCPTLQFTFMS 402
           +G+LSHA+NLYLRYKP + +L  FLEFQ+P QWAP + +LPLG +R K + P+LQF+ M 
Sbjct: 317 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 376

Query: 403 PRTYITSTQVTSSRKPLVGLRLYLEGKKSNWLALHVQHLTSLPKVMARLIGTSNESRRSQ 462
           P+ Y+ +++V S  +P+ GLR +LEGKK N LA+H+QHL++ P  +       + S    
Sbjct: 377 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL-------HLSHDDT 436

Query: 463 WIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRHDPNWLKQDGNGVYIVTGAQLISKGKWPK 522
           +   ++    G ++ PVKW  +S+VCT  V++  N  + D +   IVT A L  KG   +
Sbjct: 437 YEPIEEPVEKGYYV-PVKWGIFSHVCTYPVQY--NGARSD-DTASIVTKAWLEVKGMGMR 496

Query: 523 TILHLRLQFTHLPNCTIRRTEWAAAPEGSRKSTFLTNLSTTF-TFTQRAAADKKKQVPGA 582
            +L LRL F+   +   R++ W      SRKS   + +ST   T      A  K Q    
Sbjct: 497 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID 556

Query: 583 LNSGVYGEAPPVPVRSKKLLKYVDTAEVVKGPHDTPGHWLVTAAKLVTEGGKIGLNVKFA 612
           +NS VY   P  PV+  KLL  VDT EV++GP + PG+W+VT AKL  E GKI +  K++
Sbjct: 557 INSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYS 603

BLAST of Cp4.1LG17g10020 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 449.1 bits (1154), Expect = 5.6e-126
Identity = 246/574 (42.86%), Postives = 340/574 (59.23%), Query Frame = 0

Query: 44  ALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRCDK 103
           A++ LGRGFD+T+D RL + K  GA G RLV I++   RD+    G ++  V  DI C +
Sbjct: 24  AIQALGRGFDVTSDVRLLYCK--GAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSR 83

Query: 104 GDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDAADANYLAFDGY 163
           G+    +  V  F++M+E  N RS ++G +P G FN+MFN +G W  DAA    LA  GY
Sbjct: 84  GNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGY 143

Query: 164 FIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLISVRQ 223
           FI LY + L    LVL +E+++AVPS+WDPASL+ FI+ YGTHI+    IGG+D++ +RQ
Sbjct: 144 FIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQ 203

Query: 224 RPSSSIPPAELRRHLEDLGDILFSDKRSPS-SLHRKRDVKGITLICSKRGGDPFLHSHTS 283
             SS +P +E+  ++ D+    F +  S S +   K   K IT+I  +RGGD    SH  
Sbjct: 204 HQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDDLEQSHAR 263

Query: 284 WLQTVSFKPEVILFKFVPITSLLTGVAGSGYLSHAINLYLRYKPSLNDLQYFLEFQIPTQ 343
           W +TV   P++I   F PI SLL GV G  +L+ AI LYL YKP + DLQYFL++QI   
Sbjct: 264 WAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARA 323

Query: 344 WAPNFCELPLGHQRRKATCPTLQFTFMSPRTYITSTQVTSSRKPLVGLRLYLEGKKSNWL 403
           WAP    L    QR++  C +LQF+ M P+ +I++ QVT  RKP+ GLRL LEG K N L
Sbjct: 324 WAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRL 383

Query: 404 ALHVQHLTSLPKVMARLIGTSNESRRSQWIGTDDSDSNGRFLEPVKWKRYSNVCTSVVRH 463
           ++H+QHL SLPK++     +       +W G ++ DS  R+ EP+KWK +S+V TS + H
Sbjct: 384 SIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDS--RWFEPIKWKNFSHVSTSPIEH 443

Query: 464 DPNWLKQDGNGVYIVTGAQLISKGKW---PKTILHLRLQFTHLPNCTIRRTEWAAAPEGS 523
               +  D +GV+IVTGAQL   G W    K +LHL+L F+ +P CTIRR+ W   P   
Sbjct: 444 TETHI-GDLSGVHIVTGAQL---GVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTP--- 503

Query: 524 RKSTFLTNLSTTFTFTQRAAADKKKQVPGALNSGVYGEAPPVPVRSKKLLKYVDTAEVVK 583
                               A   +  PG  ++        V  +S KL K VD++E++K
Sbjct: 504 -------------------VASSGRLEPGGPSTS--SSTEEVSGQSGKLAKIVDSSEMLK 561

Query: 584 GPHDTPGHWLVTAAKLVTEGGKIGLNVKFALLDY 614
           GP D PGHWLVT AKL  E GKI L VK++LL+Y
Sbjct: 564 GPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Cp4.1LG17g10020 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.6e-70
Identity = 142/325 (43.69%), Postives = 194/325 (59.69%), Query Frame = 0

Query: 44  ALECLGRGFDLTNDFRLKFVKGSGAGGRRLVVIDDVNKRDIVFPDGGVVQGVSQDIRCDK 103
           A+  +G G+DL  D RLK+ KG     R L + +  +  +IV P G  +  VS+ I+CDK
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 104 GDRIRFKSDVLQFNQMSELLNQRSSIQGKVPSGYFNSMFNLSGDWFHDAADANYLAFDGY 163
           G+R+RF+SD+L F QM+E  NQ  S+ GK+PSG FN+MF  S  W  DAA    LAFDG 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 164 FIALYYLHLTASPLVLQDEVKKAVPSNWDPASLSRFIQTYGTHIIVGTAIGGQDLISVRQ 223
           FI+LY + L  S ++L++ VK+AVPS WDPA+L+RFI  YGTHIIV   +GG+D+I  +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 224 RPSSSIPPAELRRHLEDLGD-----------------------------ILFSDKRSPSS 283
           + SS + P +L++ L+++ D                             + F+D  S  S
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 284 LHRKRDVKGITLICSKRGGDPFLH-SHTSWLQTVSFKPEVILFKFVPITSLLTGVAGSGY 337
              K D      +C +RGG+   +  H  WLQTV  +P+VI   F+PITSLL GV GSG+
Sbjct: 254 YANKED---YVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGF 313