CSPI04G23250 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G23250
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	ethylene-responsive transcription factor 4
Location	Chr4: 21370993 .. 21371952 (+)
RNA-Seq Expression	CSPI04G23250
Synteny	CSPI04G23250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCCCAGTCCCTGCATGGGCCACGTGCGCTGTCCCTCGTTTATACGCTCCCTAATTTACGCTCCAAATTTACCCCTTTTTTCCTCTCTCCCTTTCTTCCTATAAAACCCTCCCCCTCCCCCTCATTTTCCCCTCAAATTTCAATTCACATCAATTTCTCTCTCACTACTACACCTAATTTCCTCTCTCCACCGCTTCTTTTCACAATATGGCTGCCGGAGACCGCTCCACATCCACCACGCCTCCCACTTCCAAAGAGATCCGCTACAGAGGCGTCAGAAAGAGGCCGTGGGGTCGCTACGCTGCCGAAATCCGAGACCCACGTAAGAAGACTAGGGTTTGGCTTGGCACATTCAATACGGCAGAAGAAGCGGCGCGTGCTTATGACGCGGCTGCGCGTGAATTTAGAGGCGCAAATGCCAAAACTAATTTCCCTCTTCAAACCGATTTCCTTATCGTTGATAACAATAACCACAGCAACTTCCATAAATCCGCTTGCAGTACTAGTCCCAGCCACAGCAGCACCGTCGAGTCCTCTTCCCCATCCCCAACGCCGTCGGATCCTTACCTCCACGGAGGCATCCACCCGCCAGGCATTTCCCCTCCAATTTTCTTCTTCGACGCGTTTGCTCAGGCGGAAAAGAATATCAGTGACTTTCGTCGGACCGTTACCGCCGCCGTCGCCGGTGCAAGCGACTCCGATTCGTCATCTGTAGGTGATTTCGACACTACGCGAAAATCCCGGCCGTTGGATTTTGACCTCAATCTTCCAGCTGTGGAAGTTGTCTGAGGATCCGTGTCTTTTTTTTTTTTTCTTTTCATTTTGAATATGAACTAATTTTTCTAATTTAATATCCGTAAAATCCATTTCAAGGATTGAATGAAATATTCGACCAGAATGAGATTAAAAAATATATATTAGAAGGTGTATTATTGTATAGATATCAAAGAATTTCTCCG

mRNA sequence

TTTCCCAGTCCCTGCATGGGCCACGTGCGCTGTCCCTCGTTTATACGCTCCCTAATTTACGCTCCAAATTTACCCCTTTTTTCCTCTCTCCCTTTCTTCCTATAAAACCCTCCCCCTCCCCCTCATTTTCCCCTCAAATTTCAATTCACATCAATTTCTCTCTCACTACTACACCTAATTTCCTCTCTCCACCGCTTCTTTTCACAATATGGCTGCCGGAGACCGCTCCACATCCACCACGCCTCCCACTTCCAAAGAGATCCGCTACAGAGGCGTCAGAAAGAGGCCGTGGGGTCGCTACGCTGCCGAAATCCGAGACCCACGTAAGAAGACTAGGGTTTGGCTTGGCACATTCAATACGGCAGAAGAAGCGGCGCGTGCTTATGACGCGGCTGCGCGTGAATTTAGAGGCGCAAATGCCAAAACTAATTTCCCTCTTCAAACCGATTTCCTTATCGTTGATAACAATAACCACAGCAACTTCCATAAATCCGCTTGCAGTACTAGTCCCAGCCACAGCAGCACCGTCGAGTCCTCTTCCCCATCCCCAACGCCGTCGGATCCTTACCTCCACGGAGGCATCCACCCGCCAGGCATTTCCCCTCCAATTTTCTTCTTCGACGCGTTTGCTCAGGCGGAAAAGAATATCAGTGACTTTCGTCGGACCGTTACCGCCGCCGTCGCCGGTGCAAGCGACTCCGATTCGTCATCTGTAGGTGATTTCGACACTACGCGAAAATCCCGGCCGTTGGATTTTGACCTCAATCTTCCAGCTGTGGAAGTTGTCTGAGGATCCGTGTCTTTTTTTTTTTTTCTTTTCATTTTGAATATGAACTAATTTTTCTAATTTAATATCCGTAAAATCCATTTCAAGGATTGAATGAAATATTCGACCAGAATGAGATTAAAAAATATATATTAGAAGGTGTATTATTGTATAGATATCAAAGAATTTCTCCG

Coding sequence (CDS)

ATGGCTGCCGGAGACCGCTCCACATCCACCACGCCTCCCACTTCCAAAGAGATCCGCTACAGAGGCGTCAGAAAGAGGCCGTGGGGTCGCTACGCTGCCGAAATCCGAGACCCACGTAAGAAGACTAGGGTTTGGCTTGGCACATTCAATACGGCAGAAGAAGCGGCGCGTGCTTATGACGCGGCTGCGCGTGAATTTAGAGGCGCAAATGCCAAAACTAATTTCCCTCTTCAAACCGATTTCCTTATCGTTGATAACAATAACCACAGCAACTTCCATAAATCCGCTTGCAGTACTAGTCCCAGCCACAGCAGCACCGTCGAGTCCTCTTCCCCATCCCCAACGCCGTCGGATCCTTACCTCCACGGAGGCATCCACCCGCCAGGCATTTCCCCTCCAATTTTCTTCTTCGACGCGTTTGCTCAGGCGGAAAAGAATATCAGTGACTTTCGTCGGACCGTTACCGCCGCCGTCGCCGGTGCAAGCGACTCCGATTCGTCATCTGTAGGTGATTTCGACACTACGCGAAAATCCCGGCCGTTGGATTTTGACCTCAATCTTCCAGCTGTGGAAGTTGTCTGA

Protein sequence

MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPYLHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRPLDFDLNLPAVEVV*

Homology

[Close]

BLAST of CSPI04G23250 vs. ExPASy Swiss-Prot
Match: O80340 (Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 8.5e-33
Identity = 105/213 (49.30%), Postives = 123/213 (57.75%), Query Frame = 0


Query: 7   STSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREF 66
           +T+ T   +KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD AAR+F
Sbjct: 12  TTNQTHNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDF 71

Query: 67  RGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPYLHGGIH 126
           RGA AKTNFP  T   + D    + F     + SPS SST++ +SP PT   P    G  
Sbjct: 72  RGAKAKTNFP--TFLELSDQKVPTGF-----ARSPSQSSTLDCASP-PTLVVPSATAGNV 131

Query: 127 PPGI-----------------SPPIFFFDAF-------AQAEKNISDFRRTV----TAAV 186
           PP +                 S P++F D          Q     S FR  V    T   
Sbjct: 132 PPQLELSLGGGGGGSCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMA 191

Query: 187 AGA-SDSDSSSVGDFD--TTRKSRPLDFDLNLP 189
            GA SDSDSSSV DF+    ++S+ LD DLNLP
Sbjct: 192 CGAQSDSDSSSVVDFEGGMEKRSQLLDLDLNLP 216

[Close]

BLAST of CSPI04G23250 vs. ExPASy Swiss-Prot
Match: Q40477 (Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.2e-26
Identity = 69/117 (58.97%), Postives = 77/117 (65.81%), Query Frame = 0


Query: 16  KEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTNF 75
           KE+ YRGVRKRPWGRYAAEIRDP KK+RVWLGTF+TAEEAA+AYD AAREFRG  AKTNF
Sbjct: 23  KEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNF 82

Query: 76  PLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPYLHGGIHPPGISP 133
           P  T+                 + SPSHSSTVESSS            G+H P  +P
Sbjct: 83  PSPTE-----------------NQSPSHSSTVESSSG---------ENGVHAPPHAP 113

[Close]

BLAST of CSPI04G23250 vs. ExPASy Swiss-Prot
Match: Q9MAI5 (Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ERF8 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.5e-26
Identity = 82/183 (44.81%), Postives = 110/183 (60.11%), Query Frame = 0


Query: 15  SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTN 74
           +KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TA++AARAYDAAAR+FRG  AKTN
Sbjct: 26  AKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAAARDFRGVKAKTN 85

Query: 75  FPLQTDFLIVDNNNHSNFHKSACSTSPSHSST-VESSSPSPTPSDPYLHGGIHPPG---- 134
           F +                     +SP+ SST V+S + +   + P+L   +   G    
Sbjct: 86  FGV------------------IVGSSPTQSSTVVDSPTAARFITPPHLELSLGGGGACRR 145

Query: 135 ---ISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFD--TTRKSRPLDFD 188
              +  P++++        N++ + +  T  V   S+S++SSV DF+    + S PLD D
Sbjct: 146 KIPLVHPVYYY--------NMATYPKMTTCGV--QSESETSSVVDFEGGAGKISPPLDLD 180

[Close]

BLAST of CSPI04G23250 vs. ExPASy Swiss-Prot
Match: Q9LW49 (Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ERF4 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.2e-25
Identity = 68/117 (58.12%), Postives = 77/117 (65.81%), Query Frame = 0


Query: 16  KEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTNF 75
           KE+ YRGVRKRPWGRYAAEIRDP KK+RVWLGTF+TAEEAA+AYD AAREFRG  AKTNF
Sbjct: 23  KEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNF 82

Query: 76  PLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPYLHGGIHPPGISP 133
           PL ++                 + S SHSST+ESSS            GIH P  +P
Sbjct: 83  PLPSE-----------------NQSTSHSSTMESSSGET---------GIHAPPHAP 113

[Close]

BLAST of CSPI04G23250 vs. ExPASy Swiss-Prot
Match: Q9SXS8 (Ethylene-responsive transcription factor 3 OS=Nicotiana tabacum OX=4097 GN=ERF3 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 88/194 (45.36%), Postives = 104/194 (53.61%), Query Frame = 0


Query: 15  SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTN 74
           SKEIR+RGVRKRPWGR+AAEIRDP KKTRVWLGTF++AE+AARAYDAAAR  RG  AKTN
Sbjct: 23  SKEIRFRGVRKRPWGRFAAEIRDPWKKTRVWLGTFDSAEDAARAYDAAARALRGPKAKTN 82

Query: 75  FPLQTDFLIVDNNNH---------SNFHK------SACSTSPSHSSTVES-SSPSPTPSD 134
           FPL        N  H         S F+       S   TS S SSTVES S P P P+ 
Sbjct: 83  FPLPYAHHHQFNQGHNPNNDPFVDSRFYPQDNPIISQRPTSSSMSSTVESFSGPRPPPAP 142

Query: 135 -PYLHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRK 189
                        SPP+   D  +  +          +++V    D +  +  D D    
Sbjct: 143 RQQTTASSRKYTRSPPVVPDDCHSDCDS---------SSSVVDHGDCEKENDNDNDNIAS 202

[Close]

BLAST of CSPI04G23250 vs. ExPASy TrEMBL
Match: A0A0A0L4P1 (Ethylene response factor 2 OS=Cucumis sativus OX=3659 GN=Csa_4G630010 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.6e-101
Identity = 192/193 (99.48%), Postives = 192/193 (99.48%), Query Frame = 0


Query: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120
           AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY
Sbjct: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120

Query: 121 LHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180
           L GGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP
Sbjct: 121 LDGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180

Query: 181 LDFDLNLPAVEVV 194
           LDFDLNLPAVEVV
Sbjct: 181 LDFDLNLPAVEVV 193

[Close]

BLAST of CSPI04G23250 vs. ExPASy TrEMBL
Match: A0A5A7VEL4 (Ethylene-responsive transcription factor 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001630 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.0e-89
Identity = 177/193 (91.71%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRSTS  PP SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120
            AAREFRGANAKTNFP+Q DFLIVD NNH+NFHKSACS+SPSHSSTVESSSPSPTPS+PY
Sbjct: 61  TAAREFRGANAKTNFPVQNDFLIVD-NNHTNFHKSACSSSPSHSSTVESSSPSPTPSNPY 120

Query: 121 LHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180
           L GGIH PGIS PIFFFDAFAQAEK ISDFRRTVTAA AGASDSDSSSV DFDTTRKSRP
Sbjct: 121 LDGGIHAPGISRPIFFFDAFAQAEK-ISDFRRTVTAAAAGASDSDSSSVVDFDTTRKSRP 180

Query: 181 LDFDLNLPAVEVV 194
           LDFDLNLPAVEVV
Sbjct: 181 LDFDLNLPAVEVV 191

[Close]

BLAST of CSPI04G23250 vs. ExPASy TrEMBL
Match: A0A1S3CLM9 (ethylene-responsive transcription factor 4 OS=Cucumis melo OX=3656 GN=LOC103501853 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.0e-89
Identity = 177/193 (91.71%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRSTS  PP SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120
            AAREFRGANAKTNFP+Q DFLIVD NNH+NFHKSACS+SPSHSSTVESSSPSPTPS+PY
Sbjct: 61  TAAREFRGANAKTNFPVQNDFLIVD-NNHTNFHKSACSSSPSHSSTVESSSPSPTPSNPY 120

Query: 121 LHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180
           L GGIH PGIS PIFFFDAFAQAEK ISDFRRTVTAA AGASDSDSSSV DFDTTRKSRP
Sbjct: 121 LDGGIHAPGISRPIFFFDAFAQAEK-ISDFRRTVTAAAAGASDSDSSSVVDFDTTRKSRP 180

Query: 181 LDFDLNLPAVEVV 194
           LDFDLNLPAVEVV
Sbjct: 181 LDFDLNLPAVEVV 191

[Close]

BLAST of CSPI04G23250 vs. ExPASy TrEMBL
Match: A0A6J1F5I9 (ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111442554 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.7e-68
Identity = 153/197 (77.66%), Postives = 159/197 (80.71%), Query Frame = 0


Query: 1   MAAGDRSTSTT----PPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAAGD+STST        SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGT NTAEEAA
Sbjct: 1   MAAGDQSTSTASAAEASNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTINTAEEAA 60

Query: 61  RAYDAAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTP 120
           RAYD AAREFRGA+AKTNFP+   FL VDNNN  NF KSACS SPS  STVES+SPSP P
Sbjct: 61  RAYDTAAREFRGADAKTNFPVPNGFLTVDNNN--NFRKSACS-SPSQCSTVESTSPSPAP 120

Query: 121 SDPYLHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTR 180
           SDP +   +H P IS PIFFFDAFAQ EKN SDFRR VT A    SDSDSSSV DFD TR
Sbjct: 121 SDPTI-TDVHAPAISRPIFFFDAFAQVEKN-SDFRRIVTGAGGSRSDSDSSSVVDFDRTR 180

Query: 181 KSRPLDFDLNLPAVEVV 194
           KSRPLDFDLNLPAVEVV
Sbjct: 181 KSRPLDFDLNLPAVEVV 192

[Close]

BLAST of CSPI04G23250 vs. ExPASy TrEMBL
Match: A0A6J1IML9 (ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111476936 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 9.3e-67
Identity = 151/197 (76.65%), Postives = 158/197 (80.20%), Query Frame = 0


Query: 1   MAAGDRSTST----TPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAA DRSTST        S+EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MAANDRSTSTVSAAVASNSEEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDAAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTP 120
           RAYD AAREFRGA+AKTNFP+  DF I+D NN  NF KSACS +PS  STVESSSPSP P
Sbjct: 61  RAYDTAAREFRGADAKTNFPVPNDFQIIDINN--NFRKSACS-NPSQCSTVESSSPSPAP 120

Query: 121 SDPYLHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTR 180
            DP L   +H P IS PIFFFDAFAQ EKN SDFRR V+ A    SDSDSSSV DFD TR
Sbjct: 121 LDPAL-TYVHAPSISRPIFFFDAFAQVEKN-SDFRRIVSVAGRSRSDSDSSSVVDFDRTR 180

Query: 181 KSRPLDFDLNLPAVEVV 194
           KSRPLDFDLNLPAVEVV
Sbjct: 181 KSRPLDFDLNLPAVEVV 192

[Close]

BLAST of CSPI04G23250 vs. NCBI nr
Match: XP_004146050.1 (ethylene-responsive transcription factor 4 [Cucumis sativus])

HSP 1 Score: 377.9 bits (969), Expect = 5.4e-101
Identity = 192/193 (99.48%), Postives = 192/193 (99.48%), Query Frame = 0


Query: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120
           AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY
Sbjct: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120

Query: 121 LHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180
           L GGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP
Sbjct: 121 LDGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180

Query: 181 LDFDLNLPAVEVV 194
           LDFDLNLPAVEVV
Sbjct: 181 LDFDLNLPAVEVV 193

[Close]

BLAST of CSPI04G23250 vs. NCBI nr
Match: KAE8649833.1 (hypothetical protein Csa_011985 [Cucumis sativus])

HSP 1 Score: 376.3 bits (965), Expect = 1.6e-100
Identity = 191/192 (99.48%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120
           AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY
Sbjct: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120

Query: 121 LHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180
           L GGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP
Sbjct: 121 LDGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180

Query: 181 LDFDLNLPAVEV 193
           LDFDLNLPAVEV
Sbjct: 181 LDFDLNLPAVEV 192

[Close]

BLAST of CSPI04G23250 vs. NCBI nr
Match: XP_008463800.1 (PREDICTED: ethylene-responsive transcription factor 4 [Cucumis melo] >KAA0066812.1 ethylene-responsive transcription factor 4 [Cucumis melo var. makuwa] >TYK27959.1 ethylene-responsive transcription factor 4 [Cucumis melo var. makuwa])

HSP 1 Score: 337.8 bits (865), Expect = 6.2e-89
Identity = 177/193 (91.71%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 1   MAAGDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRSTS  PP SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  AAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPY 120
            AAREFRGANAKTNFP+Q DFLIVD NNH+NFHKSACS+SPSHSSTVESSSPSPTPS+PY
Sbjct: 61  TAAREFRGANAKTNFPVQNDFLIVD-NNHTNFHKSACSSSPSHSSTVESSSPSPTPSNPY 120

Query: 121 LHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRP 180
           L GGIH PGIS PIFFFDAFAQAEK ISDFRRTVTAA AGASDSDSSSV DFDTTRKSRP
Sbjct: 121 LDGGIHAPGISRPIFFFDAFAQAEK-ISDFRRTVTAAAAGASDSDSSSVVDFDTTRKSRP 180

Query: 181 LDFDLNLPAVEVV 194
           LDFDLNLPAVEVV
Sbjct: 181 LDFDLNLPAVEVV 191

[Close]

BLAST of CSPI04G23250 vs. NCBI nr
Match: XP_038897001.1 (ethylene-responsive transcription factor 4 [Benincasa hispida])

HSP 1 Score: 297.7 bits (761), Expect = 7.1e-77
Identity = 166/205 (80.98%), Postives = 174/205 (84.88%), Query Frame = 0


Query: 1   MAAGDRSTST----TPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           M AGDRSTST     PP SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MVAGDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDAAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTP 120
           RAYD AAREFRGANAKTNFP+Q +FL+VDNNN  NFHKSACSTSPS SSTV+SSSPSP P
Sbjct: 61  RAYDTAAREFRGANAKTNFPIQNEFLMVDNNN--NFHKSACSTSPSQSSTVDSSSPSPAP 120

Query: 121 SDP----YLHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGA----SDSDSSS 180
           SDP    +L+   H P IS PIFFFDAFAQAE+ IS+FRR VT AVAGA    SDSDSSS
Sbjct: 121 SDPSLTHFLNSSNHAPTISRPIFFFDAFAQAER-ISEFRRNVTNAVAGAGGSVSDSDSSS 180

Query: 181 VGDFDTTRKSRPLDFDLNLPAVEVV 194
           V DFDTTRKSRPLDFDLNLPAVEVV
Sbjct: 181 VVDFDTTRKSRPLDFDLNLPAVEVV 202

[Close]

BLAST of CSPI04G23250 vs. NCBI nr
Match: XP_023536061.1 (ethylene-responsive transcription factor 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 272.7 bits (696), Expect = 2.4e-69
Identity = 155/197 (78.68%), Postives = 161/197 (81.73%), Query Frame = 0


Query: 1   MAAGDRSTST----TPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAAGD+STST        SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MAAGDKSTSTASAAVASNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDAAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTP 120
           RAYD AAREFRGA+AKTNFP+   FL VDNNN  NF KSACS SPS SSTVESSSPSP P
Sbjct: 61  RAYDTAAREFRGADAKTNFPVPNGFLTVDNNN--NFRKSACS-SPSQSSTVESSSPSPAP 120

Query: 121 SDPYLHGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTR 180
           SDP +   +H P IS PIFFF+AFAQ EKN SDFRR VT A    SDSDSSSV DFD TR
Sbjct: 121 SDPAI-THVHAPAISRPIFFFEAFAQVEKN-SDFRRIVTGAGGSRSDSDSSSVVDFDRTR 180

Query: 181 KSRPLDFDLNLPAVEVV 194
           KSRPLDFDLNLPAVEVV
Sbjct: 181 KSRPLDFDLNLPAVEVV 192

[Close]

BLAST of CSPI04G23250 vs. TAIR 10
Match: AT3G15210.1 (ethylene responsive element binding factor 4 )

HSP 1 Score: 141.7 bits (356), Expect = 6.0e-34
Identity = 105/213 (49.30%), Postives = 123/213 (57.75%), Query Frame = 0


Query: 7   STSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREF 66
           +T+ T   +KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD AAR+F
Sbjct: 12  TTNQTHNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDF 71

Query: 67  RGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPYLHGGIH 126
           RGA AKTNFP  T   + D    + F     + SPS SST++ +SP PT   P    G  
Sbjct: 72  RGAKAKTNFP--TFLELSDQKVPTGF-----ARSPSQSSTLDCASP-PTLVVPSATAGNV 131

Query: 127 PPGI-----------------SPPIFFFDAF-------AQAEKNISDFRRTV----TAAV 186
           PP +                 S P++F D          Q     S FR  V    T   
Sbjct: 132 PPQLELSLGGGGGGSCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMA 191

Query: 187 AGA-SDSDSSSVGDFD--TTRKSRPLDFDLNLP 189
            GA SDSDSSSV DF+    ++S+ LD DLNLP
Sbjct: 192 CGAQSDSDSSSVVDFEGGMEKRSQLLDLDLNLP 216

[Close]

BLAST of CSPI04G23250 vs. TAIR 10
Match: AT1G53170.1 (ethylene response factor 8 )

HSP 1 Score: 119.4 bits (298), Expect = 3.2e-27
Identity = 82/183 (44.81%), Postives = 110/183 (60.11%), Query Frame = 0


Query: 15  SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTN 74
           +KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TA++AARAYDAAAR+FRG  AKTN
Sbjct: 26  AKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAAARDFRGVKAKTN 85

Query: 75  FPLQTDFLIVDNNNHSNFHKSACSTSPSHSST-VESSSPSPTPSDPYLHGGIHPPG---- 134
           F +                     +SP+ SST V+S + +   + P+L   +   G    
Sbjct: 86  FGV------------------IVGSSPTQSSTVVDSPTAARFITPPHLELSLGGGGACRR 145

Query: 135 ---ISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFD--TTRKSRPLDFD 188
              +  P++++        N++ + +  T  V   S+S++SSV DF+    + S PLD D
Sbjct: 146 KIPLVHPVYYY--------NMATYPKMTTCGV--QSESETSSVVDFEGGAGKISPPLDLD 180

[Close]

BLAST of CSPI04G23250 vs. TAIR 10
Match: AT5G44210.1 (erf domain protein 9 )

HSP 1 Score: 114.0 bits (284), Expect = 1.3e-25
Identity = 79/191 (41.36%), Postives = 104/191 (54.45%), Query Frame = 0


Query: 4   GDRSTSTTPPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAA 63
           G   T T     KE+ +RGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD AA
Sbjct: 17  GGGKTMTMTTMRKEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAA 76

Query: 64  REFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTPSDPYLHG 123
           REFRG+ AKTNFPL  +   V++   ++ + +  + + +   T     P     +  + G
Sbjct: 77  REFRGSKAKTNFPLPGESTTVNDGGENDSYVNRTTVTTAREMT-RQRFPFACHRERKVVG 136

Query: 124 GIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAGASDSDSSSVGDFDTTRKSRPLDF 183
           G    G     FFFD  ++A    ++  R         +   S  + +       R L+ 
Sbjct: 137 GYASAG-----FFFDP-SRAASLRAELSRVCPVRFDPVNIELSIGIRETVKVEPRRELNL 196

Query: 184 DLNL--PAVEV 193
           DLNL  P V+V
Sbjct: 197 DLNLAPPVVDV 200

[Close]

BLAST of CSPI04G23250 vs. TAIR 10
Match: AT1G28370.1 (ERF domain protein 11 )

HSP 1 Score: 109.4 bits (272), Expect = 3.3e-24
Identity = 62/96 (64.58%), Postives = 69/96 (71.88%), Query Frame = 0


Query: 18  IRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTNFPL 77
           +RYRGVRKRPWGRYAAEIRDP KK+RVWLGTF+T EEAARAYD  A EFRGA AKTNFP 
Sbjct: 18  VRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYDKRAIEFRGAKAKTNFPC 77

Query: 78  QTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPS 114
                      + N H  + + S S SSTVESS P+
Sbjct: 78  Y----------NINAHCLSLTQSLSQSSTVESSFPN 103

[Close]

BLAST of CSPI04G23250 vs. TAIR 10
Match: AT1G03800.1 (ERF domain protein 10 )

HSP 1 Score: 108.6 bits (270), Expect = 5.7e-24
Identity = 51/63 (80.95%), Postives = 56/63 (88.89%), Query Frame = 0


Query: 15  SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDAAAREFRGANAKTN 74
           SKE+RYRGVR+RPWGRYAAEIRDP KK RVWLG+FNT EEAARAYD+AA  FRG+ A TN
Sbjct: 48  SKEVRYRGVRRRPWGRYAAEIRDPVKKKRVWLGSFNTGEEAARAYDSAAIRFRGSKATTN 107

Query: 75  FPL 78
           FPL
Sbjct: 108 FPL 110

The following BLAST results are available for this feature:

BLAST of CSPI04G23250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80340	8.5e-33	49.30	Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q40477	1.2e-26	58.97	Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 ...	[more]
Q9MAI5	4.5e-26	44.81	Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q9LW49	2.2e-25	58.12	Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ER...	[more]
Q9SXS8	1.1e-24	45.36	Ethylene-responsive transcription factor 3 OS=Nicotiana tabacum OX=4097 GN=ERF3 ...	[more]

BLAST of CSPI04G23250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4P1	2.6e-101	99.48	Ethylene response factor 2 OS=Cucumis sativus OX=3659 GN=Csa_4G630010 PE=4 SV=1	[more]
A0A5A7VEL4	3.0e-89	91.71	Ethylene-responsive transcription factor 4 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3CLM9	3.0e-89	91.71	ethylene-responsive transcription factor 4 OS=Cucumis melo OX=3656 GN=LOC1035018...	[more]
A0A6J1F5I9	1.7e-68	77.66	ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IML9	9.3e-67	76.65	ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of CSPI04G23250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146050.1	5.4e-101	99.48	ethylene-responsive transcription factor 4 [Cucumis sativus]	[more]
KAE8649833.1	1.6e-100	99.48	hypothetical protein Csa_011985 [Cucumis sativus]	[more]
XP_008463800.1	6.2e-89	91.71	PREDICTED: ethylene-responsive transcription factor 4 [Cucumis melo] >KAA0066812...	[more]
XP_038897001.1	7.1e-77	80.98	ethylene-responsive transcription factor 4 [Benincasa hispida]	[more]
XP_023536061.1	2.4e-69	78.68	ethylene-responsive transcription factor 4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI04G23250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15210.1	6.0e-34	49.30	ethylene responsive element binding factor 4	[more]
AT1G53170.1	3.2e-27	44.81	ethylene response factor 8	[more]
AT5G44210.1	1.3e-25	41.36	erf domain protein 9	[more]
AT1G28370.1	3.3e-24	64.58	ERF domain protein 11	[more]
AT1G03800.1	5.7e-24	80.95	ERF domain protein 10	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 20..31 score: 66.67 coord: 42..58 score: 74.65
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 19..82 e-value: 1.4E-36 score: 137.5
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 19..68 e-value: 9.5E-15 score: 54.6
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 19..76 score: 24.579044
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 19..76 e-value: 3.07516E-19 score: 75.3859
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 18..78 e-value: 1.1E-28 score: 101.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 94..117
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 94..124
None	No IPR available	PANTHER	PTHR31677	AP2 DOMAIN CLASS TRANSCRIPTION FACTOR	coord: 127..182 coord: 1..115
None	No IPR available	PANTHER	PTHR31677:SF156	ERF FAMILY PROTEIN	coord: 127..182 coord: 1..115
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 19..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G23250.1	CSPI04G23250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity