CSPI04G20110 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G20110
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase CHFR isoform X2
Location	Chr4: 18178844 .. 18182883 (-)
RNA-Seq Expression	CSPI04G20110
Synteny	CSPI04G20110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAAATGCTATCATTGTCGATGAGACTCTTGTTCAAAAGGATGAAACTACTTTCATCAAGTGTGGAAGCGAAATTGTTTCGGGTCCAGTTCGGGATGGTTAGTATTTCTTCAAACTCTTTCTTGTTTGTGAAAGAGAGAGTTTAACTGTGGATGATTTAGAATTATTTGGTTTCGCATTGTTGATATCATATTATGGAAGCCTTCTCTAGTTTGTTATCTGTAAGTATATTATTTACCATATTAGTAATCATTAAATTCTAGACTTAAAGAGCACCAGAAAGATCCAGCTTCTAATCCACATGGTAAGCAACGCACCAGAAAATCCTACCGAATCCACAGAGAACCAGCCAATGACCCTAGAGCTCTGAAATGTTCTATAAAAGCATTATTAAATCTTTTCATTTTAGATTCCTTCAGTGGTGGCAGTAACAAAATTGTGGTGACGGAAATACTTCCTAAATTGCTTTGACCAGAATAATACCACAATCTTTGACAACGGACACCAAGTCCTTTGGAAAATCCAAGGATTTTAGTGGGGAGGACCAAGGGGCATTGTCTAGCGGGACACAATCATCTTCACAAAGAATGTCTAAATCCATCCCTTCAATCTCTGCAACAGCCTTATGATCAGAAGGACACCTCAAGCCCGTTGATTCTTCACTACTAACACTGGAGGGTGAGTCTACCTCCAACTCTGCTTGCTTCTTGGTGGAAACAACAGAATGACAAAGGGGAACCTTGTAATAACGTTACCTTTGTGTTAGGGTCTGTGAAACTCGAATAATTGAAAATTTTTGGAGCAGGAATATCAGTTACTAACCTGCTGAGAGAATCAGAGGCATGGACTTTGGAAGTACACACCTTCAAGAGGTTCGGGTTATCTTCGAAAGGGGAGGGCTTGAGTTTAAGTTTCTGGTGGGGATCCAAAAGAAGGGCTTTGAGAGAACAAGCCCCAATTGATTCAGACTTTAGGAGAGCAGTTCATGTGTTGTTGAAAGACATCTTCTTTTGAATGTCAGACAAAATTTCGGGGAGTTGACTTGTTTTTTCCTTTACTCTGACTCAATCTGTTTAAATTGCTAGTTTCTGGAGAACTTTGGAATGCAGCATCTGACTGAGGTTCTTTGAGAGGGAGATCGTTGGGCCTCTGTTTTTTAAAAGGGGAATCTAACTTGGTGTAGCCCACAAAACCAACTAGACAGCCGTTCTTAATAGGTTTTACAGAATCCAATAAGGTTTTACAGAATCCAATAAAGATGACTTTTTGAATTCCAGTGCAATTAAAGCAGTTTAGGCCCAACTTCTTGCTTCTTTTGTCTTCTCCGGGGCCGATGAGGGTTCTTGAAGGTGTTCCAGAATTGCAAATGGATTTCTTGCTATGGGAAGATGTGCAAAAGTTGATTTTGGGAGATTTAAAACTGAGGGGAAAAAAGGGAGGTCTGAACCAGATTTTGCAAGGCCTCCCTAATTCTTAAAAAGTCAATAGAATTTCTAAAATCATTAACCATAATAGTACCAACAGTAGGAACGGGAGGATTCAAACATTTGAAATCACCGAAGTTTAAAAAAATGTTTCCCCTTTTAAGATCAGTAATCTCAATCGTGGAAGGTACAGAACCGCAAAGATTTTGCTTAACTTGAATCCTAATTTGACTGTAATTGGTAAGATTTAAAGTGTCCATGGCAATGCTTTCAAGTTCTCCAAAATGATCCACGATAACCTTGAAGTTTTTCCTGCTCCAGTAATCCAAAGGGAGGTTCTTTATTTTGAGCCATCCTCCATAGTCTTTCATGGTGAGCGGCCGATTGTTTGTGTTTATCCCATCTTTCAAAATTTAGGTGAAAAGAGCCCATTGCCTGCCATTTCCCTTCATTATTGATGAGATCCTTTATCGAGCCTTGGTCAAGACTAATGAGAGCATTCTCATCAATTAAAGGATTAATAACTATCTTTGCTTGGAAATAGTTCTTTAACATCTTTCGAATTCTCTTCCAATCGTCGAAGGCAAAGAGCTTAGTAGTGATCCTAAGGTTTTCAAAATTGGTTGGGATAACTTCAAAATTCCTAATTACCCAATTCTTTTGCTTTCCGCTATTCAAACCTGAGAAAGGACAAGGGGAAACCTTTGCTACTTTTGTATCGGCCACCGCTACAGGACTATTGCAATCTCTGGAAGCTTTCTTTATGACAGACACATTGCTCTTTTTCTGTTTGACTTTATCTGTATAACTCAGGTTGCTTTCCATATTATTGGGAATGTGCTTCAAGTAATTTCTAGAAAACCAATTGACATATTCCTCCCTAGAACAAAAATTGTCCAGCATATTTAGAAAGGATCTCCATCCCTATTTGTTTTCCCTCGAGCACACCCTTATATTAGAAAGCCCTCCTAATGAAGGCCAAAAGTCACAGCTTAATACCAATCCAGTCTTTGTTTTAAATTTCAAAGCTTTCAATCTACCTCTAACTACTTCACCTAGCTTTTGAAAGAACTTGTCCCCTGGCCCTCTTAATAATTTTGAGACCGCCGTCAACAACCATTCTAGATGAAATCTGGAGACCGACAAGATTCTACAAGCTTCCACATCTTCAACATGAATCTGATTTCCCACTTTCCAAGTACAGTAATGAGACTCGAGGACTTTGCAGTTGACTACTTCCATATTTGGATGAAAATGCCGGCAGCCGAAAGGAAAGAGGATAGAGGGAGGAGAGAGGAGGGAGGAGGGAGAGAACTTACCTTTCTTCCCTTAATGACATCGTATTGTTTTGGACCATCTATACTTCCTCAATGTCATTTAATTTCTCTATTTTCGAATATGATGAGGACGCTAAGGGGGTGTCAGCCTAGTTGAGATGTCCGGGTGCGTCTATTGATCTCTAGTTCTTTAATTGCTCATTGTATAACCCTTTTGTACTTTGAGCTTTCCTCATTTTTTATAATAAATGAGATTCGTTTCCTCATTGTACTGTTTTGGACCATCTATACTTCCTCAATGTCATTTAATTTCTCTATTTTTCGGATATGATGAGGACGTGAAGGGGGTGTCAGCCTAGTTGAGATGTCCTGGTGCGTCTATTAATCGCTAGTTCTTTATTGCTCATTCTGTAACCCTTTTGTATGAGCTTTCCTCATTTTTTTATAATAAATGAGATTCGTTTCCTTCTTTTAAACAAATATTAGGTTGTTAATTAATAGATGAGCTAAGTAAATAAATTAATTTTTCTTAGTTTCTTTGACTTCTAACAACACTTGCAAAATCCTTCTGTATTTTTGTCAACTCTTTTTTATAAGAACTAATCAATTCTTGTTTAATTTAAGCCAATTGGAGAGTATCTTATGCTTACCTTTGGTTTTGGCTTTGAATATTTTCAGGAATCTTTTCTTATTCAATTTTTCTTATAGCGAAATCCGAAAGCTAACTGCACAGATATTTTTTATGGTTTGATTGAAAATGTAGAACTGCTTGTCTTATGGAGCTTTTCGATTGTAATTTCCAGGGAATTTGAGCTTTAAATTTGAAATGTTGTCAGATTCAAAATTATGCAAAGGCTTAAAGGTGATTGGTAATTGTCATGTTTTGGTAATTTGGTTATCAGAATGTGATATTAGGTTCTAACCATCCTTTTAGAAATACTCAATTCTAGGGGTTGTTTGAATTTGTCCAGATTTCTGTGGATGTTGAGCACGCAAAATGCAGTATTTGCTTAAACATATGGCATGATGTTGTCACAGCCGCTCCTTGCTTCCACAACTTTTGGTGGGCCTGTTAGTTTGTTTTTTGGTTGGCTAGTTCGTCTTTCAATACAGTTATTTTCATTTAATTGTGTTAGTTGACAGCAATGGATGTTTCTCGGAGTGGTTAAAAAGATCCCAAGAGAAGCATACAAGTGTACTTTGTCCCCAATGTAGAGCAGTCGTGCAGTTTGTTGGAAGAAACCACTACTTGCTGAACATTGCGGAGGTAATATCGTACTTAAGCATCTTACTGAAGAAAACTGTTGATTGGTATGAGTTAGAGCGAAACAAGAAATAA

mRNA sequence

TTCAAATGCTATCATTGTCGATGAGACTCTTGTTCAAAAGGATGAAACTACTTTCATCAAGTGTGGAAGCGAAATTGTTTCGGGTCCAGTTCGGGATGGGAATTTGAGCTTTAAATTTGAAATGTTGTCAGATTCAAAATTATGCAAAGGCTTAAAGATTTCTGTGGATGTTGAGCACGCAAAATGCAGTATTTGCTTAAACATATGGCATGATGTTGTCACAGCCGCTCCTTGCTTCCACAACTTTTGCAATGGATGTTTCTCGGAGTGGTTAAAAAGATCCCAAGAGAAGCATACAAGTGTACTTTGTCCCCAATGTAGAGCAGTCGTGCAGTTTGTTGGAAGAAACCACTACTTGCTGAACATTGCGGAGGTAATATCGTACTTAAGCATCTTACTGAAGAAAACTGTTGATTGGTATGAGTTAGAGCGAAACAAGAAATAA

Coding sequence (CDS)

TCAAATGCTATCATTGTCGATGAGACTCTTGTTCAAAAGGATGAAACTACTTTCATCAAGTGTGGAAGCGAAATTGTTTCGGGTCCAGTTCGGGATGGGAATTTGAGCTTTAAATTTGAAATGTTGTCAGATTCAAAATTATGCAAAGGCTTAAAGATTTCTGTGGATGTTGAGCACGCAAAATGCAGTATTTGCTTAAACATATGGCATGATGTTGTCACAGCCGCTCCTTGCTTCCACAACTTTTGCAATGGATGTTTCTCGGAGTGGTTAAAAAGATCCCAAGAGAAGCATACAAGTGTACTTTGTCCCCAATGTAGAGCAGTCGTGCAGTTTGTTGGAAGAAACCACTACTTGCTGAACATTGCGGAGGTAATATCGTACTTAAGCATCTTACTGAAGAAAACTGTTGATTGGTATGAGTTAGAGCGAAACAAGAAATAA

Protein sequence

SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLLNIAEVISYLSILLKKTVDWYELERNKK*

Homology

[Close]

BLAST of CSPI04G20110 vs. ExPASy Swiss-Prot
Match: Q810L3 (E3 ubiquitin-protein ligase CHFR OS=Mus musculus OX=10090 GN=Chfr PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-10
Identity = 35/104 (33.65%), Postives = 49/104 (47.12%), Query Frame = 0


Query: 32  DGNLSFKFEMLSDSKLCKGLKISVDVEHAK-----------CSICLNIWHDVVTAAPCFH 91
           DG L    ++L   +       S DV+ A            C IC ++ HD V+  PC H
Sbjct: 262 DGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMH 321

Query: 92  NFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLLNIAE 125
            FC  C+S W++RS       LCP CR  V+ + +NH L N+ E
Sbjct: 322 TFCAACYSGWMERSS------LCPTCRCPVERICKNHILNNLVE 359

[Close]

BLAST of CSPI04G20110 vs. ExPASy Swiss-Prot
Match: Q5FWP4 (E3 ubiquitin-protein ligase CHFR OS=Xenopus laevis OX=8355 GN=chfr PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.5e-10
Identity = 31/82 (37.80%), Postives = 41/82 (50.00%), Query Frame = 0


Query: 43  SDSKLCKGLKISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVL 102
           SD      +K     E   C IC  + HD V+  PC H FC  C+S W++RS       L
Sbjct: 247 SDESKTPSMKPDKMEETLTCIICQELLHDCVSLQPCMHTFCAACYSGWMERSS------L 306

Query: 103 CPQCRAVVQFVGRNHYLLNIAE 125
           CP CR  V+ + +NH L N+ E
Sbjct: 307 CPTCRCPVERICKNHILNNLVE 322

[Close]

BLAST of CSPI04G20110 vs. ExPASy Swiss-Prot
Match: Q6P256 (E3 ubiquitin-protein ligase CHFR OS=Xenopus tropicalis OX=8364 GN=chfr PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.5e-10
Identity = 31/82 (37.80%), Postives = 41/82 (50.00%), Query Frame = 0


Query: 43  SDSKLCKGLKISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVL 102
           SD      +K     E   C IC  + HD V+  PC H FC  C+S W++RS       L
Sbjct: 248 SDESKTPSMKPDKMEETLTCIICQELLHDCVSLQPCMHTFCAACYSGWMERSS------L 307

Query: 103 CPQCRAVVQFVGRNHYLLNIAE 125
           CP CR  V+ + +NH L N+ E
Sbjct: 308 CPTCRCPVERICKNHILNNLVE 323

[Close]

BLAST of CSPI04G20110 vs. ExPASy Swiss-Prot
Match: Q96EP1 (E3 ubiquitin-protein ligase CHFR OS=Homo sapiens OX=9606 GN=CHFR PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 5.9e-10
Identity = 28/67 (41.79%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 58  EHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNH 117
           E   C IC ++ HD V+  PC H FC  C+S W++RS       LCP CR  V+ + +NH
Sbjct: 300 ETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSS------LCPTCRCPVERICKNH 359

Query: 118 YLLNIAE 125
            L N+ E
Sbjct: 360 ILNNLVE 360

[Close]

BLAST of CSPI04G20110 vs. ExPASy Swiss-Prot
Match: Q5RF77 (E3 ubiquitin-protein ligase CHFR OS=Pongo abelii OX=9601 GN=CHFR PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.9e-10
Identity = 28/67 (41.79%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 58  EHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNH 117
           E   C IC ++ HD V+  PC H FC  C+S W++RS       LCP CR  V+ + +NH
Sbjct: 208 ETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSS------LCPTCRCPVERICKNH 267

Query: 118 YLLNIAE 125
            L N+ E
Sbjct: 268 ILNNLVE 268

[Close]

BLAST of CSPI04G20110 vs. ExPASy TrEMBL
Match: A0A0A0KYR0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508530 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.9e-68
Identity = 126/138 (91.30%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 60
           SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA
Sbjct: 72  SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 131

Query: 61  KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL 120
           KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHT+VLCPQCRAVVQFVGRNHYLL
Sbjct: 132 KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTNVLCPQCRAVVQFVGRNHYLL 191

Query: 121 NIAEVISYLSILLKKTVD 139
           NIAE I      L+++V+
Sbjct: 192 NIAEDILQADSSLRRSVE 209

[Close]

BLAST of CSPI04G20110 vs. ExPASy TrEMBL
Match: A0A1S3B4V7 (E3 ubiquitin-protein ligase CHFR isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486049 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.7e-66
Identity = 123/138 (89.13%), Postives = 129/138 (93.48%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 60
           SNAIIVDETLVQKDETTFI+CGSEIVSGPVRDG+LSFKFEM SDSKLCKGLKISVDVEHA
Sbjct: 72  SNAIIVDETLVQKDETTFIRCGSEIVSGPVRDGHLSFKFEMSSDSKLCKGLKISVDVEHA 131

Query: 61  KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL 120
           KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRA VQFVGRNHYLL
Sbjct: 132 KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAAVQFVGRNHYLL 191

Query: 121 NIAEVISYLSILLKKTVD 139
           NIAE I      L+++V+
Sbjct: 192 NIAEDILQADSSLRRSVE 209

[Close]

BLAST of CSPI04G20110 vs. ExPASy TrEMBL
Match: A0A1S4DUC8 (E3 ubiquitin-protein ligase CHFR isoform X3 OS=Cucumis melo OX=3656 GN=LOC103486049 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 4.3e-64
Identity = 123/146 (84.25%), Postives = 129/146 (88.36%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLK-------- 60
           SNAIIVDETLVQKDETTFI+CGSEIVSGPVRDG+LSFKFEM SDSKLCKGLK        
Sbjct: 72  SNAIIVDETLVQKDETTFIRCGSEIVSGPVRDGHLSFKFEMSSDSKLCKGLKVIGNCHVL 131

Query: 61  ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQF 120
           ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRA VQF
Sbjct: 132 ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAAVQF 191

Query: 121 VGRNHYLLNIAEVISYLSILLKKTVD 139
           VGRNHYLLNIAE I      L+++V+
Sbjct: 192 VGRNHYLLNIAEDILQADSSLRRSVE 217

[Close]

BLAST of CSPI04G20110 vs. ExPASy TrEMBL
Match: A0A1S3B4G1 (E3 ubiquitin-protein ligase CHFR isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486049 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 4.3e-64
Identity = 123/146 (84.25%), Postives = 129/146 (88.36%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLK-------- 60
           SNAIIVDETLVQKDETTFI+CGSEIVSGPVRDG+LSFKFEM SDSKLCKGLK        
Sbjct: 72  SNAIIVDETLVQKDETTFIRCGSEIVSGPVRDGHLSFKFEMSSDSKLCKGLKVIGNCHVL 131

Query: 61  ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQF 120
           ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRA VQF
Sbjct: 132 ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAAVQF 191

Query: 121 VGRNHYLLNIAEVISYLSILLKKTVD 139
           VGRNHYLLNIAE I      L+++V+
Sbjct: 192 VGRNHYLLNIAEDILQADSSLRRSVE 217

[Close]

BLAST of CSPI04G20110 vs. ExPASy TrEMBL
Match: A0A6J1F3R3 (E3 ubiquitin-protein ligase CHFR isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439866 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 9.0e-62
Identity = 113/137 (82.48%), Postives = 126/137 (91.97%), Query Frame = 0


Query: 2   NAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHAK 61
           NAI+VDETLVQKD+  FIKCGSEIVSGPV+DG+LS+KFE+LSDSK+CKGLKIS+DVEH K
Sbjct: 65  NAILVDETLVQKDDIAFIKCGSEIVSGPVQDGHLSYKFEILSDSKVCKGLKISMDVEHTK 124

Query: 62  CSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLLN 121
           CSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEK TSVLCPQCRAVVQFVGRNH+LLN
Sbjct: 125 CSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKRTSVLCPQCRAVVQFVGRNHFLLN 184

Query: 122 IAEVISYLSILLKKTVD 139
           IAE I      L+++V+
Sbjct: 185 IAETILQEDSSLRRSVE 201

[Close]

BLAST of CSPI04G20110 vs. NCBI nr
Match: XP_004146401.2 (E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis sativus] >KAE8649715.1 hypothetical protein Csa_012000 [Cucumis sativus])

HSP 1 Score: 266.9 bits (681), Expect = 1.0e-67
Identity = 126/138 (91.30%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 60
           SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA
Sbjct: 72  SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 131

Query: 61  KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL 120
           KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHT+VLCPQCRAVVQFVGRNHYLL
Sbjct: 132 KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTNVLCPQCRAVVQFVGRNHYLL 191

Query: 121 NIAEVISYLSILLKKTVD 139
           NIAE I      L+++V+
Sbjct: 192 NIAEDILQADSSLRRSVE 209

[Close]

BLAST of CSPI04G20110 vs. NCBI nr
Match: XP_031739668.1 (E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 125/126 (99.21%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 60
           SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA
Sbjct: 72  SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 131

Query: 61  KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL 120
           KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHT+VLCPQCRAVVQFVGRNHYLL
Sbjct: 132 KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTNVLCPQCRAVVQFVGRNHYLL 191

Query: 121 NIAEVI 127
           NIAEVI
Sbjct: 192 NIAEVI 197

[Close]

BLAST of CSPI04G20110 vs. NCBI nr
Match: XP_008442086.1 (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis melo])

HSP 1 Score: 261.2 bits (666), Expect = 5.6e-66
Identity = 123/138 (89.13%), Postives = 129/138 (93.48%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 60
           SNAIIVDETLVQKDETTFI+CGSEIVSGPVRDG+LSFKFEM SDSKLCKGLKISVDVEHA
Sbjct: 72  SNAIIVDETLVQKDETTFIRCGSEIVSGPVRDGHLSFKFEMSSDSKLCKGLKISVDVEHA 131

Query: 61  KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL 120
           KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRA VQFVGRNHYLL
Sbjct: 132 KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAAVQFVGRNHYLL 191

Query: 121 NIAEVISYLSILLKKTVD 139
           NIAE I      L+++V+
Sbjct: 192 NIAEDILQADSSLRRSVE 209

[Close]

BLAST of CSPI04G20110 vs. NCBI nr
Match: XP_038887863.1 (E3 ubiquitin-protein ligase CHFR [Benincasa hispida])

HSP 1 Score: 257.3 bits (656), Expect = 8.1e-65
Identity = 119/138 (86.23%), Postives = 130/138 (94.20%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLKISVDVEHA 60
           SNAIIVDETLVQKD+TTFIKCGSEI+SGPVRDG+LS+KF+MLS S++CKGLKISVDVEHA
Sbjct: 72  SNAIIVDETLVQKDDTTFIKCGSEIISGPVRDGHLSYKFKMLSGSRICKGLKISVDVEHA 131

Query: 61  KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL 120
           KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL
Sbjct: 132 KCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYLL 191

Query: 121 NIAEVISYLSILLKKTVD 139
           NIAE I      L+++V+
Sbjct: 192 NIAEDILQADSSLRRSVE 209

[Close]

BLAST of CSPI04G20110 vs. NCBI nr
Match: XP_016899584.1 (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis melo])

HSP 1 Score: 253.8 bits (647), Expect = 8.9e-64
Identity = 123/146 (84.25%), Postives = 129/146 (88.36%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEMLSDSKLCKGLK-------- 60
           SNAIIVDETLVQKDETTFI+CGSEIVSGPVRDG+LSFKFEM SDSKLCKGLK        
Sbjct: 72  SNAIIVDETLVQKDETTFIRCGSEIVSGPVRDGHLSFKFEMSSDSKLCKGLKVIGNCHVL 131

Query: 61  ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQF 120
           ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRA VQF
Sbjct: 132 ISVDVEHAKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAAVQF 191

Query: 121 VGRNHYLLNIAEVISYLSILLKKTVD 139
           VGRNHYLLNIAE I      L+++V+
Sbjct: 192 VGRNHYLLNIAEDILQADSSLRRSVE 217

[Close]

BLAST of CSPI04G20110 vs. TAIR 10
Match: AT1G47570.1 (RING/U-box superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 3.2e-43
Identity = 79/139 (56.83%), Postives = 101/139 (72.66%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEML-SDSKLCKGLKISVDVEH 60
           S+AI+VD+ +V KD    I  GSEIV GP   G L ++F ++ +     + L+IS+D EH
Sbjct: 76  SDAILVDKAVVPKDGAVDIISGSEIVPGPEEQGYLQYRFTIMPAPESRTQLLQISIDPEH 135

Query: 61  AKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYL 120
           AKCSICLNIWHDVVTAAPC HNFCNGCFSEW++RS+EKH  VLCPQCR  VQ+VG+NH+L
Sbjct: 136 AKCSICLNIWHDVVTAAPCLHNFCNGCFSEWMRRSEEKHKHVLCPQCRTTVQYVGKNHFL 195

Query: 121 LNIAEVISYLSILLKKTVD 139
            NI E I  +   L++  +
Sbjct: 196 KNIQEEILKVDAALRRPAE 214

[Close]

BLAST of CSPI04G20110 vs. TAIR 10
Match: AT1G47570.2 (RING/U-box superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 3.2e-43
Identity = 79/139 (56.83%), Postives = 101/139 (72.66%), Query Frame = 0


Query: 1   SNAIIVDETLVQKDETTFIKCGSEIVSGPVRDGNLSFKFEML-SDSKLCKGLKISVDVEH 60
           S+AI+VD+ +V KD    I  GSEIV GP   G L ++F ++ +     + L+IS+D EH
Sbjct: 63  SDAILVDKAVVPKDGAVDIISGSEIVPGPEEQGYLQYRFTIMPAPESRTQLLQISIDPEH 122

Query: 61  AKCSICLNIWHDVVTAAPCFHNFCNGCFSEWLKRSQEKHTSVLCPQCRAVVQFVGRNHYL 120
           AKCSICLNIWHDVVTAAPC HNFCNGCFSEW++RS+EKH  VLCPQCR  VQ+VG+NH+L
Sbjct: 123 AKCSICLNIWHDVVTAAPCLHNFCNGCFSEWMRRSEEKHKHVLCPQCRTTVQYVGKNHFL 182

Query: 121 LNIAEVISYLSILLKKTVD 139
            NI E I  +   L++  +
Sbjct: 183 KNIQEEILKVDAALRRPAE 201

[Close]

BLAST of CSPI04G20110 vs. TAIR 10
Match: AT3G05250.1 (RING/U-box superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 5.0e-04
Identity = 17/50 (34.00%), Postives = 26/50 (52.00%), Query Frame = 0


Query: 62  CSICLNIWHDVVTAAPCFHNFCNGCFSEWLK----RSQEKHTSVLCPQCR 108
           C ICL  +        CFH FC  C  +W+K    ++ ++ +SV CP C+
Sbjct: 22  CPICLGQFLRESYLDTCFHKFCFNCIKQWIKVVSSKASKQRSSVTCPLCK 71

The following BLAST results are available for this feature:

BLAST of CSPI04G20110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q810L3	1.6e-10	33.65	E3 ubiquitin-protein ligase CHFR OS=Mus musculus OX=10090 GN=Chfr PE=1 SV=1	[more]
Q5FWP4	3.5e-10	37.80	E3 ubiquitin-protein ligase CHFR OS=Xenopus laevis OX=8355 GN=chfr PE=1 SV=1	[more]
Q6P256	3.5e-10	37.80	E3 ubiquitin-protein ligase CHFR OS=Xenopus tropicalis OX=8364 GN=chfr PE=2 SV=1	[more]
Q96EP1	5.9e-10	41.79	E3 ubiquitin-protein ligase CHFR OS=Homo sapiens OX=9606 GN=CHFR PE=1 SV=2	[more]
Q5RF77	5.9e-10	41.79	E3 ubiquitin-protein ligase CHFR OS=Pongo abelii OX=9601 GN=CHFR PE=2 SV=1	[more]

BLAST of CSPI04G20110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYR0	4.9e-68	91.30	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508530 P...	[more]
A0A1S3B4V7	2.7e-66	89.13	E3 ubiquitin-protein ligase CHFR isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486...	[more]
A0A1S4DUC8	4.3e-64	84.25	E3 ubiquitin-protein ligase CHFR isoform X3 OS=Cucumis melo OX=3656 GN=LOC103486...	[more]
A0A1S3B4G1	4.3e-64	84.25	E3 ubiquitin-protein ligase CHFR isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486...	[more]
A0A6J1F3R3	9.0e-62	82.48	E3 ubiquitin-protein ligase CHFR isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of CSPI04G20110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146401.2	1.0e-67	91.30	E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis sativus] >KAE8649715.1 hypo...	[more]
XP_031739668.1	1.3e-67	99.21	E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis sativus]	[more]
XP_008442086.1	5.6e-66	89.13	PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis melo]	[more]
XP_038887863.1	8.1e-65	86.23	E3 ubiquitin-protein ligase CHFR [Benincasa hispida]	[more]
XP_016899584.1	8.9e-64	84.25	PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis melo]	[more]

BLAST of CSPI04G20110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47570.1	3.2e-43	56.83	RING/U-box superfamily protein	[more]
AT1G47570.2	3.2e-43	56.83	RING/U-box superfamily protein	[more]
AT3G05250.1	5.0e-04	34.00	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 62..106 e-value: 1.9E-5 score: 34.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 61..107 e-value: 3.8E-8 score: 33.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 62..107 score: 12.046688
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 51..124 e-value: 3.4E-15 score: 57.2
None	No IPR available	PANTHER	PTHR16079	UBIQUITIN LIGASE PROTEIN CHFR	coord: 1..126
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 61..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G20110.1	CSPI04G20110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding