CSPI04G16230 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G16230
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionmacrophage migration inhibitory factor homolog
LocationChr4: 13516687 .. 13519649 (-)
RNA-Seq ExpressionCSPI04G16230
SyntenyCSPI04G16230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAACGAGGGCAGACGATTCTTCAGATAAGAATTCACTGAACACACAAGGAGGAGAAGAAGAAGAAGAAGAAGAAGAAGATGCCTTGCCTTAACATTTCTACAAATGTCAACCTTGAAGGTATCGACACCTCCTCTGTTCTTTCTGAAGCTTCATCTACCGTTGCCAAGATCATTGGCAAGCCAGAAGCCGTACGTTTCTAATTTTCTCTATTTAATTACCATTCACCTTACAACTGTTTTTGTACTCATTAAGGCTTATTCTTCACTTTTCTGTTTGATTTTAGAGCGATTGGTGATGTTTTTTTTTTATCGATTTGAGAATCTTTGACTTGACTGTCATTTTAGACTGTGCACGGGTAGAATCAGCTATTGGGATGCTATTTTAGGGTTTCTAGATCTTTGTTAAGATGGAAGTTAGAATGTTGCTGTTACTTATAGTTGTGATTATAGCTTTGTAGTTTAGTCAACCTAGCTTTGCAACTGTTTAGAGTACTTGGTTATCTTCTTCTTGAAAGGGGTAACGGAAAAACAGTCCTTTTTTTTTATGTATATATATATACACACGCTGGGCCGTCTTATGAATAGTTCTACTAATGTCACGAGACAACCTCACTTACCCTACAACATTAGTTCTACTAATCTCACGAGAAAATCTGCTTGACTCTACAACACTTATGTGTCAAAGAAACTCAGAGGAACCTAGGTAGGTGGCCAGATTGAACCCATGCAGAGTTTGCTATTGAGACTATGTCTTCCTCTTTCTCTTTACCATTGGACCCTAATTCTCTTTACCATTAGACCCTAATTTATGATGATTCAAAAACACAGTTTTAGAAGAGCCATACTGAACCATGAAGTGACTCCAATGTAACCTAACTAGAAATCTAACCTAGCTTAGGACCAGATCTCTTACCAAACTTCTCAATCCTTTTGAAATCCTATTATTGTTCTCTAAGTCCCTCATTTAGTAACAAAAGAATTGGCTCTCCAAGGAGTGCTCCTGTCCTGTTTTCAGGGTGTAGCAACAATTGTTCCATTATATGGCATCAATCCCCATTACAAGTCAGGCTCACCCCATAATTGGGCAAGAAAACTAGTGCTGTTTTTGTTTTTGTTTTCTTCCACATTTGTGAGTGTTTGGCCTTGAATCTCGTCTAATCTCACGAGATAACTTGCTTGCCCTATAATATTTGAGTGCCAAGAAAATTTCTAGGATATTAAATTGTAGGTTGGTGACCATCATGTATTGAAATCCTTCTCTTCAAGTTTTTTTATTATATCAATGGTCCTTATTAGGTGAGGCCAACCTATGTTGGTGAAGAAAGCTGGCAGTTACCTGACCCAAATCCTCAAACTCCTCCTTGTGGAGAATATACCATCGAAGTTTCAATGCAAAAGGAAGAATATCTCTAAATATGATCCATACTGTTGAGTTGTTAACACTCTATGAAAATCCTACATTCAAAGAATTTAACCTTTTAAAATATTTGTCTTACCTAACAAGGAGAGGACCAAGGAGGAAGGGAACCAAGAGCTGCAGAAAAGGCTGAAAAAGGAACTGCAAGAGAAGAGAAGCCGTATTCAAGCAACCTACTCTTAGCTCCATTGGAGTTGAATTTCCTCATCTCAAGAATGCCTTTTTTTTTTCCTTTTTACAAGAAATGCCATTTATTCTTTAAGAATTTGAACCTGATAATTTTTACAAATAATATATGTGAAGAGAGAAGCAACTTGAAATGTTGATGTTCCCTCATGTCCATATTGGTGTTGATGAAGGGATTCTCTCTGAATCAAGGAATGAAGTGGTGTATTTGCTATTCTCCTTTGATCCTGGTTGAGCTTCAGCTTCTTCTGTGGCTGGTTTTTAAGCTAGTAGTTAAGAGTACCTTCTTTTCACTTTCATTCAAAGAAGATGAGGTCCATAGACCCTACAGTCACAACACATACAGCATAAGTGAATAAATATTTATTCATCCAGACATTTTATTCTTAGCAATTTAACCTGTTATTAATTCATGTGTGTGACTCCAGTTACGAGATTTGTTGAGGATCTTTATAGAAATTGTGTGCTGATGTGACAATAGATTGGTTTTTGTCAAGTTCTTTCTGATGCGTTAACTTGAACATCTTGCTGCAAGCAGTACGTAATGATTGTGCTGAAGGGATCGGTACCGATGTCATTTGGGGGCAGTGAGCAGCCAGCAGCATATGGTGAGTTGGTGTCCATCGGTGGTCTGAACCCTGATGTCAACAAGAAATTGAGTGCTGCAATTTCTGCAATCCTGGAGACCAAGCTATCTGTTCCCAAGTCAAGATTCTTTCTCAAATTCTACGACACCAAGGCAAGTTATTCTATCTTTTTCTCAAGATGGGTTACTGAAATGCATGCACTTCTCATTGCATTTCTTATTTTTGTTTAGGCCCATCAGAGTCAAGAATATGCACAGTGTTTACATGCTTTACACCAGAACTAGAGATCATGTAACTAAATCTTTTTTTCTATTTTGAAACAAATGTGCTCAACTCCTGGCTGTCTTTTGCAAACATTTGAATCACTTTTAGACACTAATTTCACATTGACTTCTCTTAAATGGCTTATGATAAACCTGGGAATGGTACTGAGATAATAATTTTTTATGGCTGCTGCAGGGTTCCAACTTGGGATGGAATGGCTCCACTTTCTAAGTTAACTTCATCTGTAAGCTCTTGAAATTCCCAACTTTTATATTTGCTGGGACCTAGTTATAAGGATTTGGTATTGTTAACCCTTCATATTATATTGAGTTCTGTCTAGATTCCCTGTGACTTTTGGTTATGAATCATAGATCGAGTTATAATCTACCAAGTTTTAATGAGTACTTTTATTGGTTTAACACTCTTAACATTTTTAATCATTTTGAAAGACATACCTTAATACCTTAAGAAGTTTGCAGAATATACCTTTAATTGTTTTTTATTTTT

mRNA sequence

GAAAACGAGGGCAGACGATTCTTCAGATAAGAATTCACTGAACACACAAGGAGGAGAAGAAGAAGAAGAAGAAGAAGAAGATGCCTTGCCTTAACATTTCTACAAATGTCAACCTTGAAGGTATCGACACCTCCTCTGTTCTTTCTGAAGCTTCATCTACCGTTGCCAAGATCATTGGCAAGCCAGAAGCCTACGTAATGATTGTGCTGAAGGGATCGGTACCGATGTCATTTGGGGGCAGTGAGCAGCCAGCAGCATATGGTGAGTTGGTGTCCATCGGTGGTCTGAACCCTGATGTCAACAAGAAATTGAGTGCTGCAATTTCTGCAATCCTGGAGACCAAGCTATCTGTTCCCAAGTCAAGATTCTTTCTCAAATTCTACGACACCAAGGGTTCCAACTTGGGATGGAATGGCTCCACTTTCTAAGTTAACTTCATCTGTAAGCTCTTGAAATTCCCAACTTTTATATTTGCTGGGACCTAGTTATAAGGATTTGGTATTGTTAACCCTTCATATTATATTGAGTTCTGTCTAGATTCCCTGTGACTTTTGGTTATGAATCATAGATCGAGTTATAATCTACCAAGTTTTAATGAGTACTTTTATTGGTTTAACACTCTTAACATTTTTAATCATTTTGAAAGACATACCTTAATACCTTAAGAAGTTTGCAGAATATACCTTTAATTGTTTTTTATTTTT

Coding sequence (CDS)

ATGCCTTGCCTTAACATTTCTACAAATGTCAACCTTGAAGGTATCGACACCTCCTCTGTTCTTTCTGAAGCTTCATCTACCGTTGCCAAGATCATTGGCAAGCCAGAAGCCTACGTAATGATTGTGCTGAAGGGATCGGTACCGATGTCATTTGGGGGCAGTGAGCAGCCAGCAGCATATGGTGAGTTGGTGTCCATCGGTGGTCTGAACCCTGATGTCAACAAGAAATTGAGTGCTGCAATTTCTGCAATCCTGGAGACCAAGCTATCTGTTCCCAAGTCAAGATTCTTTCTCAAATTCTACGACACCAAGGGTTCCAACTTGGGATGGAATGGCTCCACTTTCTAA

Protein sequence

MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF*
Homology
[Close]
BLAST of CSPI04G16230 vs. ExPASy Swiss-Prot
Match: P81529 (Macrophage migration inhibitory factor homolog OS=Trichinella spiralis OX=6334 PE=1 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 1.6e-18
Identity = 46/115 (40.00%), Postives = 70/115 (60.87%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MP   ++TN+    +  S  LS  S+ V  I+ KP +YV + +     +SFGGS +PAA+
Sbjct: 1   MPIFTLNTNIKATDV-PSDFLSSTSALVGNILSKPGSYVAVHINTDQQLSFGGSTKPAAF 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           G L+SIGG+ P  N+  SA +   L  KL +PK+R ++ F +  G ++GWNG+TF
Sbjct: 61  GTLMSIGGIEPSRNRDHSAKLFDHLNKKLGIPKNRMYIHFVNLNGDDVGWNGTTF 114

[Close]
BLAST of CSPI04G16230 vs. ExPASy Swiss-Prot
Match: P81748 (Macrophage migration inhibitory factor homolog OS=Trichuris trichiura OX=36087 PE=1 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 6.0e-18
Identity = 45/115 (39.13%), Postives = 71/115 (61.74%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MP    STNV  E I     L   S  +A ++GKPE+YV + + G   ++FGG++ PA +
Sbjct: 1   MPIFTFSTNVPSENISV-DFLKSTSKLIAGMLGKPESYVAVHINGGQKITFGGTDAPAGF 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           G+L+S+GG+  + N+  SA +   L   L +P +R ++ F D +GS++G+NGSTF
Sbjct: 61  GQLLSLGGVGGEKNRSHSAKLFKHLTDGLGIPGNRMYINFVDMRGSDVGYNGSTF 114

[Close]
BLAST of CSPI04G16230 vs. ExPASy Swiss-Prot
Match: P91850 (Macrophage migration inhibitory factor homolog OS=Brugia malayi OX=6279 GN=Bm1_28435 PE=3 SV=4)

HSP 1 Score: 90.1 bits (222), Expect = 1.8e-17
Identity = 49/115 (42.61%), Postives = 71/115 (61.74%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MP   I TN+    I +S+ L +AS+ VAK +GKPE+YV I + G   M FGGSE P A 
Sbjct: 1   MPYFTIDTNIPQNSI-SSAFLKKASNVVAKALGKPESYVSIHVNGGQAMVFGGSEDPCAV 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
             L SIG + P VN   +  +  +L  +L +PK+R +++F D + S++ +NGSTF
Sbjct: 61  CVLKSIGCVGPKVNNSHAEKLYKLLADELKIPKNRCYIEFVDIEASSMAFNGSTF 114

[Close]
BLAST of CSPI04G16230 vs. ExPASy Swiss-Prot
Match: O44786 (Macrophage migration inhibitory factor homolog OS=Wuchereria bancrofti OX=6293 GN=MIF PE=3 SV=3)

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-15
Identity = 46/115 (40.00%), Postives = 68/115 (59.13%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MP   I TN   + I +S+ L +A + V K +GKPE+YV I + G  PM FGGSE P   
Sbjct: 1   MPYFTIDTNKPQDSI-SSAFLKKAPNVVPKALGKPESYVSIHVNGGQPMVFGGSEDPCPV 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
             L SIG + P VN   +  +  +L  +L +PK+R +++  D + S++ +NGSTF
Sbjct: 61  CVLKSIGCVGPKVNNSHAEKLYKLLADELKIPKNRCYIESVDIEASSMAFNGSTF 114

[Close]
BLAST of CSPI04G16230 vs. ExPASy Swiss-Prot
Match: Q76BK2 (Macrophage migration inhibitory factor OS=Xenopus laevis OX=8355 GN=mif PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.8e-14
Identity = 43/115 (37.39%), Postives = 64/115 (55.65%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MP   I TNV  + +   ++LS+ +  +AK  GKP  Y+ I +     MSFG S  P A 
Sbjct: 1   MPVFTIRTNVCRDSV-PDTLLSDLTKQLAKATGKPAEYIAIHIVPDQIMSFGDSTDPCAV 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
             L SIG +    NK  +  +  IL  +L++P +R ++ +YD   +N+GWNGSTF
Sbjct: 61  CSLCSIGKIGGPQNKSYTKLLCDILTKQLNIPANRVYINYYDLNAANVGWNGSTF 114

[Close]
BLAST of CSPI04G16230 vs. ExPASy TrEMBL
Match: A0A0A0L2R6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G314430 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.2e-55
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY
Sbjct: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. ExPASy TrEMBL
Match: A0A6J1HRS5 (macrophage migration inhibitory factor homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466128 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.4e-54
Identity = 111/115 (96.52%), Postives = 114/115 (99.13%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAY
Sbjct: 1   MPCLNVSTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMVVLKGSVPMSFGGSEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKFYDTKGSN GWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAVSAILETKLSVPKSRFFLKFYDTKGSNFGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. ExPASy TrEMBL
Match: A0A6J1EB33 (macrophage migration inhibitory factor homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432434 PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.9e-54
Identity = 111/115 (96.52%), Postives = 114/115 (99.13%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAY
Sbjct: 1   MPCLNLSTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMVVLKGSVPMSFGGSEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKFYDTKGSN GWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAVSAILETKLSVPKSRFFLKFYDTKGSNFGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. ExPASy TrEMBL
Match: A0A6J1CSE6 (macrophage migration inhibitory factor homolog OS=Momordica charantia OX=3673 GN=LOC111013871 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.0e-52
Identity = 107/115 (93.04%), Postives = 113/115 (98.26%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNL+G+DTSSVLSEASSTVA+IIGKPEAYVM+VLKGSVPMSFGGSEQPAAY
Sbjct: 1   MPCLNVSTNVNLDGMDTSSVLSEASSTVAEIIGKPEAYVMVVLKGSVPMSFGGSEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAA+S ILETKLSVPKSRFFLKFYDTKGSN GWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAVSDILETKLSVPKSRFFLKFYDTKGSNFGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. ExPASy TrEMBL
Match: A0A1S3TFC2 (macrophage migration inhibitory factor homolog isoform X2 OS=Vigna radiata var. radiata OX=3916 GN=LOC106754911 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.9e-52
Identity = 105/115 (91.30%), Postives = 114/115 (99.13%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNL+G+DTSS+LSEA+STVA IIGKPEAYVMIVLKGSVP+SFGG+EQPAAY
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEATSTVASIIGKPEAYVMIVLKGSVPISFGGNEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAAI+AILETKLSVPKSR+FLKFYDTKGSNLGWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAIAAILETKLSVPKSRYFLKFYDTKGSNLGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. NCBI nr
Match: XP_011653708.1 (macrophage migration inhibitory factor homolog isoform X2 [Cucumis sativus])

HSP 1 Score: 224.6 bits (571), Expect = 4.5e-55
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY
Sbjct: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. NCBI nr
Match: XP_022966490.1 (macrophage migration inhibitory factor homolog isoform X2 [Cucurbita maxima])

HSP 1 Score: 221.9 bits (564), Expect = 2.9e-54
Identity = 111/115 (96.52%), Postives = 114/115 (99.13%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAY
Sbjct: 1   MPCLNVSTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMVVLKGSVPMSFGGSEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKFYDTKGSN GWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAVSAILETKLSVPKSRFFLKFYDTKGSNFGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. NCBI nr
Match: XP_038881028.1 (macrophage migration inhibitory factor homolog [Benincasa hispida])

HSP 1 Score: 221.9 bits (564), Expect = 2.9e-54
Identity = 112/115 (97.39%), Postives = 115/115 (100.00%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLNISTNVNLEG+DTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGG+EQPAAY
Sbjct: 1   MPCLNISTNVNLEGVDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGNEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNG+TF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGTTF 115

[Close]
BLAST of CSPI04G16230 vs. NCBI nr
Match: XP_022925084.1 (macrophage migration inhibitory factor homolog isoform X2 [Cucurbita moschata] >XP_023517014.1 macrophage migration inhibitory factor homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.5 bits (563), Expect = 3.8e-54
Identity = 111/115 (96.52%), Postives = 114/115 (99.13%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAY
Sbjct: 1   MPCLNLSTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMVVLKGSVPMSFGGSEQPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKFYDTKGSN GWNGSTF
Sbjct: 61  GELVSIGGLNPDVNKKLSAAVSAILETKLSVPKSRFFLKFYDTKGSNFGWNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. NCBI nr
Match: KAG6595575.1 (hypothetical protein SDJN03_12128, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 220.3 bits (560), Expect = 8.6e-54
Identity = 110/115 (95.65%), Postives = 114/115 (99.13%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNLEGIDTS+VLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAY
Sbjct: 19  MPCLNLSTNVNLEGIDTSAVLSEASSTVAKIIGKPEAYVMVVLKGSVPMSFGGSEQPAAY 78

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKFYDTKGSN GWNGSTF
Sbjct: 79  GELVSIGGLNPDVNKKLSAAVSAILETKLSVPKSRFFLKFYDTKGSNFGWNGSTF 133

[Close]
BLAST of CSPI04G16230 vs. TAIR 10
Match: AT5G01650.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 204.5 bits (519), Expect = 4.5e-53
Identity = 102/114 (89.47%), Postives = 109/114 (95.61%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNL+G+DTSS+LSEASSTVAKIIGKPE YVMIVLKGSVPMSFGG+E PAAY
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGKPENYVMIVLKGSVPMSFGGTEDPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGST 115
           GELVSIGGLN DVNKKLSAA+SAILETKLSVPKSRFFLKFYDTKGS  GWNG+T
Sbjct: 61  GELVSIGGLNADVNKKLSAAVSAILETKLSVPKSRFFLKFYDTKGSFFGWNGAT 114

[Close]
BLAST of CSPI04G16230 vs. TAIR 10
Match: AT5G01650.2 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 4.4e-48
Identity = 95/104 (91.35%), Postives = 101/104 (97.12%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCLN+STNVNL+G+DTSS+LSEASSTVAKIIGKPE YVMIVLKGSVPMSFGG+E PAAY
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGKPENYVMIVLKGSVPMSFGGTEDPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTK 105
           GELVSIGGLN DVNKKLSAA+SAILETKLSVPKSRFFLKFYDTK
Sbjct: 61  GELVSIGGLNADVNKKLSAAVSAILETKLSVPKSRFFLKFYDTK 104

[Close]
BLAST of CSPI04G16230 vs. TAIR 10
Match: AT5G57170.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 5.2e-33
Identity = 66/115 (57.39%), Postives = 87/115 (75.65%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MP LN+ TN+ ++ +  S +L +A+  VAKIIGKPE+YVMI+L   VP++F G+E+PAAY
Sbjct: 1   MPTLNLFTNIPVDAVTCSDILKDATKAVAKIIGKPESYVMILLNSGVPIAFAGTEEPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGWNGSTF 116
           GEL+SIGGL P VN KLS  IS IL+ KLS+  SRF++KFYD+     G+NGSTF
Sbjct: 61  GELISIGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKFYDSPRPFFGYNGSTF 115

[Close]
BLAST of CSPI04G16230 vs. TAIR 10
Match: AT5G57170.2 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 5.4e-30
Identity = 62/108 (57.41%), Postives = 82/108 (75.93%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MP LN+ TN+ ++ +  S +L +A+  VAKIIGKPE+YVMI+L   VP++F G+E+PAAY
Sbjct: 1   MPTLNLFTNIPVDAVTCSDILKDATKAVAKIIGKPESYVMILLNSGVPIAFAGTEEPAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNL 109
           GEL+SIGGL P VN KLS  IS IL+ KLS+  SRF++KFYD+  S L
Sbjct: 61  GELISIGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKFYDSPVSPL 108

[Close]
BLAST of CSPI04G16230 vs. TAIR 10
Match: AT3G51660.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 1.9e-22
Identity = 48/102 (47.06%), Postives = 71/102 (69.61%), Query Frame = 0 

Query: 1   MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAY 60
           MPCL I+TNVN +G++T    SE +  VA I+G+P+  VM+VLKGSV + FGG+++ AAY
Sbjct: 1   MPCLYITTNVNFDGVNTDPFYSEVTKAVASIVGRPQNLVMVVLKGSVEIVFGGNKEAAAY 60

Query: 61  GELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYD 103
            E+VS+GG+   V ++L A + +IL T  S+  +RF  K +D
Sbjct: 61  AEIVSMGGITKQVKRELIATVGSILHTHFSIHPTRFIFKVFD 102

The following BLAST results are available for this feature:
BLAST of CSPI04G16230 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P815291.6e-1840.00Macrophage migration inhibitory factor homolog OS=Trichinella spiralis OX=6334 P... [more]
P817486.0e-1839.13Macrophage migration inhibitory factor homolog OS=Trichuris trichiura OX=36087 P... [more]
P918501.8e-1742.61Macrophage migration inhibitory factor homolog OS=Brugia malayi OX=6279 GN=Bm1_2... [more]
O447862.1e-1540.00Macrophage migration inhibitory factor homolog OS=Wuchereria bancrofti OX=6293 G... [more]
Q76BK21.8e-1437.39Macrophage migration inhibitory factor OS=Xenopus laevis OX=8355 GN=mif PE=1 SV=... [more]
BLAST of CSPI04G16230 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L2R62.2e-55100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G314430 PE=3 SV=1[more]
A0A6J1HRS51.4e-5496.52macrophage migration inhibitory factor homolog isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1EB331.9e-5496.52macrophage migration inhibitory factor homolog isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1CSE61.0e-5293.04macrophage migration inhibitory factor homolog OS=Momordica charantia OX=3673 GN... [more]
A0A1S3TFC23.9e-5291.30macrophage migration inhibitory factor homolog isoform X2 OS=Vigna radiata var. ... [more]
BLAST of CSPI04G16230 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011653708.14.5e-55100.00macrophage migration inhibitory factor homolog isoform X2 [Cucumis sativus][more]
XP_022966490.12.9e-5496.52macrophage migration inhibitory factor homolog isoform X2 [Cucurbita maxima][more]
XP_038881028.12.9e-5497.39macrophage migration inhibitory factor homolog [Benincasa hispida][more]
XP_022925084.13.8e-5496.52macrophage migration inhibitory factor homolog isoform X2 [Cucurbita moschata] >... [more]
KAG6595575.18.6e-5495.65hypothetical protein SDJN03_12128, partial [Cucurbita argyrosperma subsp. sorori... [more]
BLAST of CSPI04G16230 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G01650.14.5e-5389.47Tautomerase/MIF superfamily protein [more]
AT5G01650.24.4e-4891.35Tautomerase/MIF superfamily protein [more]
AT5G57170.15.2e-3357.39Tautomerase/MIF superfamily protein [more]
AT5G57170.25.4e-3057.41Tautomerase/MIF superfamily protein [more]
AT3G51660.11.9e-2247.06Tautomerase/MIF superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014347Tautomerase/MIF superfamilyGENE3D3.30.429.10Macrophage Migration Inhibitory Factorcoord: 2..115
e-value: 1.4E-43
score: 149.9
IPR014347Tautomerase/MIF superfamilySUPERFAMILY55331Tautomerase/MIFcoord: 1..115
IPR001398Macrophage migration inhibitory factorPFAMPF01187MIFcoord: 2..115
e-value: 4.9E-24
score: 84.8
IPR001398Macrophage migration inhibitory factorPANTHERPTHR11954D-DOPACHROME DECARBOXYLASEcoord: 1..115
NoneNo IPR availablePANTHERPTHR11954:SF42TAUTOMERASE/MIF SUPERFAMILY PROTEINcoord: 1..115

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G16230.1CSPI04G16230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005615 extracellular space
molecular_function GO:0050178 phenylpyruvate tautomerase activity