CSPI04G12500 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G12500
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMediator of RNA polymerase II transcription subunit 21
LocationChr4: 10916688 .. 10919325 (+)
RNA-Seq ExpressionCSPI04G12500
SyntenyCSPI04G12500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGAAACACCAATTAATTATTATATGTGAAAATTATTGAAAATAGTTAGAAATACATCTCAATAATACTTTAATTCATTCTGCTATTTTCGAAACATTTTGTGATTGACGACTTGACGTTCTAAAACTAGAAAATTAGGAAGGTATAAGGAAAAGAAGGAACCCTAATTCATCTGCTTTTTCTCCGACTCACGTTCAACCGCCCAGCCCCTTCCTTGTCTCCGGTAGCATCTCGATGACAGTCCTTTGAGATAAACGGCGGATCGTACTCTCACTCACTCAGTTTCTGGAACTGATTTGCTGATTAAAAGGAGCGATATGTGTATTATTTGCGGAATCATCTTCTGAACAATTCATCCTAGGGATAAGGTTGAAGATGGATATAATTTCCCAATTGCAGGAACAAGTTAACACAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTGCAAAGAGATGCTCCTCCAGTTCGTTTATCCCCTAATTATCCCGAACCACCGTCTCAGGAGCCTACAGAGGATAATGCAACCATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAGCAGGTGCGTATCATTTATTCTGCACTTTAATACTTCGGGGTGGAAAATATGAATGAGTTAGAGTACCTCTTGTTAATAAACTTTCCACGCATTGAAACATCAAGAAAAATATTTGCATTTTCCATTTGAGGAAATTCAGTGTCCTAGATTAACTTTACATGCATTCTGTCTAGTGTCTACTGGGTAAGTAGCCGAGAATGCAGTATCTACTTGAAGCACCCTTGTGTTGAGGTTGGAAGAGAAAGAAAAATCAAGGCAGCAGGTTTCATGCTACTTGTTTGAGATGAGTACATATTCTAATGCTATGATATATGATACAATCCACAAATTTGGTATTGAAAACTTCAGTCTATTTTTCCCATCTAACATCAAATACTACTGAGGTAGGTATGGCTATTCGATCGGCAATAAAACAAAGTACTATGGTTCTTTTTTTTTAGTTATCCTTTTACTTTTTAACTGCTTGTTATGTATATTTAGGCTTCTTTAAGTGTTGATATTTTGGAATCTGATACTCAAACCCTGTATTTGAGCAATTTTGCTGAGAGAGTTTTGATTTTCACAAGTTTCCATCGTCGAAATCAGCAATGCATTTAGGTTTGTTCTTCCGAATAATCTTTTAAGGATTATATAGAATTATAAAATAGACTGATTCTGTCCACGTTAAGTTTGAAAATCTCAGTGTGATAGTATTAAGTAATAGAAACGTACAAAGTTTTCCAACTCTGCTGGACTTTTAATGACTTCTAGATGTGATTCTACAACGAGTTAGATTTGTTCTATATATTCTCTCATTTTCTGTATTTTTTACGTTTACCTCTTGATTGGTATGTTATTTTATTAGTTTTTTGAGGAGTTGCAAGGAGCCAACTGAGGAATTTCTCCTCCATCCACTTTTCTCTCTCGGAGAAATGGCTGTTTTCATAGTAGGTTGGAGTATATGCTAATTTGTGGGGTTTGGGGTGCGAGAGAAATGATAGAATCTTCAAGGGAGAAATAACCGAATCTTGGATCTTGAAGATTCTTTTTTAATAATTAATCGCTTAGTCACATTTCACTTGATTGGAGCCCTTCCCCTTCTTATAGTTAGCTACACTTTCTGTGAACACCATTCTGTATGGTTATGTATTCTTTCACCTTGTTTTTCAACAAAAGTCTAGTTGTACATCAAAAACAAAATTTATCAGCATTTTGAACAAAAGAAAGGGATGGGATCTTGCTTCATTTATTTGCAATTATCCAATTGAACATGTGTACCTTTTCTTAAAAAAATTATTGTCAATATTTTCTAGCACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGTATGAATTCTCATATTCTGATGTTGATATAAGATCAAGAGTTGTTGTGTTGTTTACTTCTCTCAGTATGTATTTCAGGCTGAGAACGATGTTGTAGGTCAAGAACTTCAAAAACAGCTACAAGCAGCAGGTGTTATCTCTGTCTTCTTGTTAATCTCTACATTTTCAAACCGTGCCCCGGTGTGGTTACATAAGACTGATGTTGTTTGGCTGGCGACTTACTTTAGTTGAAATAATTCTATTTAATAATTTTAGCCCTAAATGTAAATTTCTCATGTTCACTTCATTCTATTGAACATAAGGATATTTTGCGCAACTAACAATCTTACCGAAACTGTGTTTCAGAGAAAGAATTAGTACAAGTTCAGGAGCTGTTCAGCCAAGTGGCAGATAATTGCTTGAACTTAAAGAAAGCAGATTGAAAGAGTGGGGGAATTTTCCTATGCTACCAATGTATGGAAATTTTCCGTATCTCCCCGTTCATTTCTGCCGCATGACTTTTTATTTTTATATTACATATATGTAGAAATATACGTGAATCTACAGTTTATGGAATAAAAATACTTTTATCCATTGTATTAATTTTTTTTATGACGGATTGATATTTTCATCAATATTTCTACGTTTTCAAAACGGTTTTGATATTAATATGAGGAGTGAATGGATATTTTCTGTTAGATT

mRNA sequence

CTGAAACACCAATTAATTATTATATGTGAAAATTATTGAAAATAGTTAGAAATACATCTCAATAATACTTTAATTCATTCTGCTATTTTCGAAACATTTTGTGATTGACGACTTGACGTTCTAAAACTAGAAAATTAGGAAGGTATAAGGAAAAGAAGGAACCCTAATTCATCTGCTTTTTCTCCGACTCACGTTCAACCGCCCAGCCCCTTCCTTGTCTCCGGTAGCATCTCGATGACAGTCCTTTGAGATAAACGGCGGATCGTACTCTCACTCACTCAGTTTCTGGAACTGATTTGCTGATTAAAAGGAGCGATATGTGTATTATTTGCGGAATCATCTTCTGAACAATTCATCCTAGGGATAAGGTTGAAGATGGATATAATTTCCCAATTGCAGGAACAAGTTAACACAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTGCAAAGAGATGCTCCTCCAGTTCGTTTATCCCCTAATTATCCCGAACCACCGTCTCAGGAGCCTACAGAGGATAATGCAACCATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAGCAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGTTGTAGGTCAAGAACTTCAAAAACAGCTACAAGCAGCAGAGAAAGAATTAGTACAAGTTCAGGAGCTGTTCAGCCAAGTGGCAGATAATTGCTTGAACTTAAAGAAAGCAGATTGAAAGAGTGGGGGAATTTTCCTATGCTACCAATGTATGGAAATTTTCCGTATCTCCCCGTTCATTTCTGCCGCATGACTTTTTATTTTTATATTACATATATGTAGAAATATACGTGAATCTACAGTTTATGGAATAAAAATACTTTTATCCATTGTATTAATTTTTTTTATGACGGATTGATATTTTCATCAATATTTCTACGTTTTCAAAACGGTTTTGATATTAATATGAGGAGTGAATGGATATTTTCTGTTAGATT

Coding sequence (CDS)

ATGGATATAATTTCCCAATTGCAGGAACAAGTTAACACAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTGCAAAGAGATGCTCCTCCAGTTCGTTTATCCCCTAATTATCCCGAACCACCGTCTCAGGAGCCTACAGAGGATAATGCAACCATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAGCAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGTTGTAGGTCAAGAACTTCAAAAACAGCTACAAGCAGCAGAGAAAGAATTAGTACAAGTTCAGGAGCTGTTCAGCCAAGTGGCAGATAATTGCTTGAACTTAAAGAAAGCAGATTGA

Protein sequence

MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQVQELFSQVADNCLNLKKAD*
Homology
BLAST of CSPI04G12500 vs. ExPASy Swiss-Prot
Match: C0LU16 (Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana OX=3702 GN=MED21 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-51
Identity = 110/139 (79.14%), Postives = 120/139 (86.33%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNAT-IAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP+     D+AT   EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQ 120
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END+VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLKKAD 139
           VQELF Q ADNCLN+KK +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

BLAST of CSPI04G12500 vs. ExPASy Swiss-Prot
Match: Q7ZTI5 (Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN=med21 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 2.7e-04
Identity = 37/139 (26.62%), Postives = 68/139 (48.92%), Query Frame = 0

Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPP-----VRLSPNYPEPPSQEPTEDNATIAEQ 61
           D ++QLQ+ VN++A    N  G LQ+ APP     ++ + N  +P    PTE+ A     
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQP--SNPTEEYA----- 62

Query: 62  PKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKE 121
            +L +A + + AK  D L+ +LP  +     Q   + +L+ EN      L++ +   +  
Sbjct: 63  -QLFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENQEAAARLEEVVYRGDAL 122

Query: 122 LVQVQELFSQVADNCLNLK 136
           L ++Q   + +A + L  +
Sbjct: 123 LEKIQTALADIAQSQLRTR 133

BLAST of CSPI04G12500 vs. ExPASy TrEMBL
Match: A0A0A0KZ00 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis sativus OX=3659 GN=Csa_4G268030 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.7e-65
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. ExPASy TrEMBL
Match: A0A5A7TEZ9 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00920 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 4.1e-64
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTED+ATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAE+ELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. ExPASy TrEMBL
Match: A0A1S3BN38 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo OX=3656 GN=LOC103491637 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 4.1e-64
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTED+ATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAE+ELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. ExPASy TrEMBL
Match: A0A5D3DEB7 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001120 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.3e-64
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTED ATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDGATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAE+ELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. ExPASy TrEMBL
Match: A0A6J1DHM9 (Mediator of RNA polymerase II transcription subunit 21 OS=Momordica charantia OX=3673 GN=LOC111021179 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.2e-61
Identity = 131/138 (94.93%), Postives = 133/138 (96.38%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS NYPEPPSQEPTED+A IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSSNYPEPPSQEPTEDSANIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. NCBI nr
Match: XP_004149640.1 (mediator of RNA polymerase II transcription subunit 21 [Cucumis sativus] >KGN54019.1 hypothetical protein Csa_021664 [Cucumis sativus])

HSP 1 Score: 257.3 bits (656), Expect = 7.6e-65
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. NCBI nr
Match: XP_008449895.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo] >KAA0040117.1 mediator of RNA polymerase II transcription subunit 21 [Cucumis melo var. makuwa])

HSP 1 Score: 253.8 bits (647), Expect = 8.4e-64
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTED+ATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAE+ELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. NCBI nr
Match: TYK21740.1 (mediator of RNA polymerase II transcription subunit 21 [Cucumis melo var. makuwa])

HSP 1 Score: 253.4 bits (646), Expect = 1.1e-63
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTED ATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDGATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAE+ELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. NCBI nr
Match: XP_038902781.1 (mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida])

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 133/138 (96.38%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVN+IAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTED+A IAEQPKLM
Sbjct: 1   MDIISQLQEQVNSIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSANIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAEKEL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. NCBI nr
Match: XP_038893979.1 (mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida] >XP_038893980.1 mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida])

HSP 1 Score: 246.5 bits (628), Expect = 1.3e-61
Identity = 131/138 (94.93%), Postives = 134/138 (97.10%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTED+A IAEQPKLM
Sbjct: 1   MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAE+EL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAERELIQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

BLAST of CSPI04G12500 vs. TAIR 10
Match: AT4G04780.1 (mediator 21 )

HSP 1 Score: 203.4 bits (516), Expect = 1.2e-52
Identity = 110/139 (79.14%), Postives = 120/139 (86.33%), Query Frame = 0

Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNAT-IAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP+     D+AT   EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQ 120
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END+VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLKKAD 139
           VQELF Q ADNCLN+KK +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0LU161.7e-5179.14Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana O... [more]
Q7ZTI52.7e-0426.62Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0KZ003.7e-65100.00Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis sativus OX=365... [more]
A0A5A7TEZ94.1e-6498.55Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. maku... [more]
A0A1S3BN384.1e-6498.55Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo OX=3656 G... [more]
A0A5D3DEB75.3e-6498.55Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. maku... [more]
A0A6J1DHM94.2e-6194.93Mediator of RNA polymerase II transcription subunit 21 OS=Momordica charantia OX... [more]
Match NameE-valueIdentityDescription
XP_004149640.17.6e-65100.00mediator of RNA polymerase II transcription subunit 21 [Cucumis sativus] >KGN540... [more]
XP_008449895.18.4e-6498.55PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo]... [more]
TYK21740.11.1e-6398.55mediator of RNA polymerase II transcription subunit 21 [Cucumis melo var. makuwa... [more]
XP_038902781.11.2e-6296.38mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida][more]
XP_038893979.11.3e-6194.93mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida] ... [more]
Match NameE-valueIdentityDescription
AT4G04780.11.2e-5279.14mediator 21 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 92..130
NoneNo IPR availableGENE3D6.10.280.10Mediator complex, subunit Med21coord: 1..130
e-value: 6.7E-29
score: 102.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..50
IPR021384Mediator complex, subunit Med21PFAMPF11221Med21coord: 1..128
e-value: 1.4E-27
score: 96.7
IPR021384Mediator complex, subunit Med21PANTHERPTHR13381RNA POLYMERASE II HOLOENZYME COMPONENT SRB7coord: 1..136
IPR037212Mediator complex, subunit Med7/Med21-likeSUPERFAMILY140718Mediator hinge subcomplex-likecoord: 1..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G12500.1CSPI04G12500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050832 defense response to fungus
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016592 mediator complex
cellular_component GO:0043078 polar nucleus
molecular_function GO:0003712 transcription coregulator activity