Cp4.1LG15g00260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG15g00260 vs. ExPASy Swiss-Prot
Match: F4IQV7 (Preprotein translocase subunit SCY2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SCY2 PE=2 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 4.4e-218
Identity = 395/541 (73.01%), Postives = 451/541 (83.36%), Query Frame = 0

Query: 40  RSAAVINFNSLKFKGRNNVLLSRPFVSKVNEKSFDC-LSNQCRRSRMNVNATSEQCLDLE 99
           R     N N  + +  +++ ++R  +     KSF    S++ R   M+        LD+E
Sbjct: 38  RKCLTTNLNMSRTRQGHSIQMNRRHLLMKERKSFSINYSDKFRDDSMSSEEMHTDALDVE 97

Query: 100 TFSQELNDGDAFFTSPNGTNSDTWK-PRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRR 159
               +  D      S N   S+T +  R K FRNRFL+ VR+ SVLN AAE FFKSEIRR
Sbjct: 98  IIPPDSQD---IRNSQNSAVSNTLQDDRPKSFRNRFLDFVRISSVLNTAAERFFKSEIRR 157

Query: 160 RLFVTAVLIVISRIGYFIPLPGYDRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGIS 219
           RLFVTAVL+V+SR+GYFIPLPG+DRRLIPQDYLSFVSGSV+ELG+F  E+K+S FQLG+S
Sbjct: 158 RLFVTAVLLVLSRVGYFIPLPGFDRRLIPQDYLSFVSGSVEELGEFGAEIKLSLFQLGLS 217

Query: 220 PQIIASIIMQVLCHILPSLVKLRKEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYS 279
           PQIIASIIMQVLCH+LPSLVKLRKEGLDG EKIKSYIWW+S  FAIVEALV++  SL YS
Sbjct: 218 PQIIASIIMQVLCHVLPSLVKLRKEGLDGHEKIKSYIWWLSFFFAIVEALVVAYTSLQYS 277

Query: 280 IYAANHRVKHVMVTTFLLVCGAMTMTWICDTISESGFGQGSSLIICIGILSGYTETLYKM 339
           ++AA  +VKHVM+T+ LLVCGAMTMTW+CDTISESGFG GSSLIIC+GIL+GYTETL+KM
Sbjct: 278 VFAATAQVKHVMMTSSLLVCGAMTMTWLCDTISESGFGHGSSLIICVGILTGYTETLHKM 337

Query: 340 LSQLSGRAVIWWPYVLALLGIFTVVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITE 399
           L+Q+SG    W PY+L LLGIFTVVTM+AVVVTEGCRKIKLQYYGFKLASA+RE SPITE
Sbjct: 338 LNQISGSFSNWLPYLLGLLGIFTVVTMFAVVVTEGCRKIKLQYYGFKLASASREGSPITE 397

Query: 400 VEPYIPFNINPAGMQPILTTTYLLAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYY 459
           VEPYIPFNINPAGMQP+LTTTYLLAFPSILAS+L S F +++KEILNPES++GA PWVYY
Sbjct: 398 VEPYIPFNINPAGMQPVLTTTYLLAFPSILASILGSPFLLNMKEILNPESTVGAPPWVYY 457

Query: 460 LIYAVFVFLFNIFDIANLPKEISDYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLL 519
            IYA FVFLFNIFDIANLPKEI+DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLL
Sbjct: 458 SIYAFFVFLFNIFDIANLPKEIADYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLL 517

Query: 520 LSFLATTSSLLDHYLRSINAGFAIGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 579
           LSFLAT S++LDHYLRSIN GF+IGFTSVLIIVGSIIELRRSY AYNVMPSLS AL+RYG
Sbjct: 518 LSFLATASTVLDHYLRSINQGFSIGFTSVLIIVGSIIELRRSYHAYNVMPSLSKALKRYG 575

BLAST of Cp4.1LG15g00260 vs. ExPASy Swiss-Prot
Match: P51297 (Protein translocase subunit SecY OS=Porphyra purpurea OX=2787 GN=secY PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 3.8e-44
Identity = 132/427 (30.91%), Postives = 226/427 (52.93%), Query Frame = 0

Query: 152 KSEIRRRLFVTAVLIVISRIGYFIPLPGYD--------RRLIPQDYLSFVSGSVDELGDF 211
           KS++R R+  T +L+V++R+G FIP+PG D         +    ++L+  SG     G F
Sbjct: 4   KSDLRNRIKFTLLLLVLARLGIFIPVPGIDHDAFYASVEKNTLVNFLNIFSG-----GGF 63

Query: 212 TTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLRK-EGLDGQEKIKSYIWWMSLGFA 271
           +T   +  F LGI P I +SI+MQ+L  I+P L KL+K EG  G++KI     +++LG+A
Sbjct: 64  ST---IGIFALGIVPYINSSIVMQLLTKIIPDLEKLQKEEGELGRQKITQITRYLALGWA 123

Query: 272 IVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAMTMTWICDTISESGFGQGSSLII 331
            +++  +S +  P   Y  N     V  +   L  G+M + W+ + I+E G G G+SL+I
Sbjct: 124 TLQSGAISIWVKP---YVFNWNFTFVCESVLALTAGSMIIMWLSELITEKGIGNGASLLI 183

Query: 332 CIGILSG----YTETLYKMLSQLSGRAVIWWPYVLALLGIFTVVTMWAVVVTEGCRKIKL 391
              I+SG    +T++ +   +  S  ++ +  +++    IF ++ +  + V EG R+IK+
Sbjct: 184 FQNIVSGLPKNFTQSFFD--ANYSNTSIKFGLFIV----IFLLMIIITIFVQEGTRRIKI 243

Query: 392 QYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYLLAFPSILASVLNSTFWVH 451
                 +++     S I +   Y+P  +N  G+ PI+  +  +A P+ L  +  +TF + 
Sbjct: 244 ------ISARQLGKSSILDPNSYLPLKLNQGGVMPIVFASASMALPAYLTQLTQNTFLLQ 303

Query: 452 VKEILNPESSIGAEPWVYYLIYAVFVFLFNIF--DIANLPKEISDYLNKMGARIPNIKPG 511
           V  +  P  S+      Y ++Y+V +  F+ F   I   P++I+  L KMGA IPNI+PG
Sbjct: 304 VLYLFCPNGSL------YLVLYSVLILFFSYFYTSIVMNPEDIATNLKKMGASIPNIRPG 363

Query: 512 KATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFAIGFTSVLIIVGSIIEL 564
           +ATI+YL  I     F G   L  +A    +++   +  N    +G TS+LI+VG  I+ 
Sbjct: 364 QATIDYLQVILNRLTFLGATFLFTVALIPFIIEKVAQIQNLR-GLGATSLLILVGVAIDT 400

BLAST of Cp4.1LG15g00260 vs. ExPASy Swiss-Prot
Match: P77964 (Protein translocase subunit SecY OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=secY PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.4e-43
Identity = 142/455 (31.21%), Postives = 229/455 (50.33%), Query Frame = 0

Query: 136 LVRLRSVLNNAAESFFK----SEIRRRLFVTAVLIVISRIGYFIPLPGYDRRLIPQ---- 195
           +V  R    +A E+F +    + +R RL +T  L+++ R+G FIP+P  DR+   Q    
Sbjct: 1   MVVSRDKAPSAQETFLQMAQAAGLRGRLLITIGLLILVRVGIFIPVPDIDRQAFSQAIND 60

Query: 196 ----DYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLRK-E 255
                +L+  +G     G  +T   +  F LGI P I ASIIMQ+L   +P+L  L+K E
Sbjct: 61  NSVIGFLNIFTG-----GGLST---VGIFALGILPYINASIIMQLLTAAIPALEDLQKNE 120

Query: 256 GLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAMTM 315
           G  G+ KI  Y  +++ G+ I++ L L+   L    YA N+    +  T   +  G+M +
Sbjct: 121 GEAGRRKISQYSRYIAFGWCIIQGLGLTVGLL--RPYANNYGPLFIFQTVLAITAGSMFV 180

Query: 316 TWICDTISESGFGQGSSLIICIGILSGYTETLYKML--SQLSGRAVIWWPYVLALLGIFT 375
            WI + I+E G G G+SL+I + I++   +TL + +  +Q  GR  I    V+ L+ +F 
Sbjct: 181 MWISELITERGIGNGASLLIFVNIVATLPQTLGQTIEYAQSGGRQSI--TAVVLLMLVFL 240

Query: 376 VVTMWAVVVTEGCRKIKLQYYGFKLASAARE--DSPITEVEPYIPFNINPAGMQPILTTT 435
           V+ +  V V EG R+I        + SA R+       E   Y+P  +N  G+ PI+  +
Sbjct: 241 VMIVGIVFVQEGTRRI-------PIISARRQVGKKLYRERTSYLPLRLNQGGVMPIIFAS 300

Query: 436 YLLAFPSILASVLNST--------FWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIF 495
            +L  PS LA               +V +   L P +      WVY ++Y+V +F F+ F
Sbjct: 301 AVLILPSSLAGFATGNEGLGGFGEIFVQISNALRPGT------WVYTVVYSVMIFFFSYF 360

Query: 496 DIANL--PKEISDYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLL 555
             + +  P+++S  L KMG+ IP I+PGK T +YL  +     F G + LSF+AT    +
Sbjct: 361 YASLIVNPEDVSKNLKKMGSSIPGIRPGKKTEQYLEGVLNRLTFLGAIFLSFVATLPIFV 420

Query: 556 DHYLRSINAGFAIGFTSVLIIVGSIIELRRSYQAY 564
           +     +     +G TS+LI+VG  I+  +  Q Y
Sbjct: 421 EQ-ATGVTTFQGLGATSLLILVGVAIDTAKQIQTY 429

BLAST of Cp4.1LG15g00260 vs. ExPASy Swiss-Prot
Match: O66491 (Protein translocase subunit SecY OS=Aquifex aeolicus (strain VF5) OX=224324 GN=secY PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 5.4e-43
Identity = 133/437 (30.43%), Postives = 221/437 (50.57%), Query Frame = 0

Query: 150 FFKSEIRRRLFVTAVLIVISRIGYFIPLPGYDRRLIPQDYLSFVSGSVDELGDFTTE--- 209
           F   E+R++   T ++ VI R+G  IP+PG +   + +D+L    GSV  L D  +    
Sbjct: 9   FELKELRQKFIFTLLMFVIYRLGSHIPIPGINPEAL-RDFLKAFEGSVFALYDIFSGGNL 68

Query: 210 MKMSFFQLGISPQIIASIIMQVLCHILPSLVKLRKEGLD-GQEKIKSYIWWMSLGFAIVE 269
            +++ F LG+ P I ASI+MQ+L   +PSL +L KE  D G+ KI  Y  +++L  A V+
Sbjct: 69  GRLTVFALGVMPYISASIMMQLLTVAIPSLQRLAKEEGDYGRYKINEYTKYLTLFVATVQ 128

Query: 270 ALVLSCY----SLPYSI-YAANHRVKHVMVTTFLLVCGAMTMTWICDTISESGFGQGSSL 329
           +L ++ +      P  I    N  +  +++T   LV G M + WI D I+E G G G+SL
Sbjct: 129 SLGIAFWIRGQVSPKGIPVVENPGISFILITVLTLVAGTMFLVWIADRITEKGIGNGASL 188

Query: 330 IICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFTVVTMWAVVVTEGCRKIKLQY 389
           II  GI++ +   + +   ++     I    +L ++ +   + +  V V E  R+I +QY
Sbjct: 189 IIFAGIVANFPNAVIQFYEKVK-TGDIGPLTLLLIIALIIAIIVGIVYVQEAERRIPIQY 248

Query: 390 YGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYLLAFPSILASVLNSTFWVHVK 449
            G ++             + Y+P  INPAG+ PI+    LL  PS L + + + F   + 
Sbjct: 249 PGRQVGRQLYAGR-----KTYLPIKINPAGVIPIIFAQALLLIPSTLLNFVQNPFIKVIA 308

Query: 450 EILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANL--PKEISDYLNKMGARIPNIKPGKA 509
           ++  P +        Y  +Y  F+  F  F  A L  P E+++ L+K GA IP ++PG+ 
Sbjct: 309 DMFQPGAIF------YNFLYVTFIVFFTYFYTAVLINPVELAENLHKAGAFIPGVRPGQD 368

Query: 510 TIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFAIGFTSVLIIVGSIIELRR 569
           T++YL +I     F+G L LS +A    L+  +    N  F  G T+ LI+VG  ++  R
Sbjct: 369 TVKYLERIINRLIFFGALFLSVIALIPILISVW---FNIPFYFGGTTALIVVGVALDTFR 428

Query: 570 SYQAYNVMPSLSNALRR 576
             + Y +     + +RR
Sbjct: 429 QIETYLIQKKYKSYVRR 429

BLAST of Cp4.1LG15g00260 vs. ExPASy Swiss-Prot
Match: Q1XDJ1 (Protein translocase subunit SecY OS=Pyropia yezoensis OX=2788 GN=secY PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 7.1e-43
Identity = 130/424 (30.66%), Postives = 218/424 (51.42%), Query Frame = 0

Query: 152 KSEIRRRLFVTAVLIVISRIGYFIPLPGYD--------RRLIPQDYLSFVSGSVDELGDF 211
           KS++R R+  T  L+V++R+G FIP+PG D         +    ++L+  SG     G F
Sbjct: 4   KSDLRNRIIFTLFLLVLARLGIFIPVPGIDHDAFYASVEKNTLVNFLNIFSG-----GGF 63

Query: 212 TTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLRK-EGLDGQEKIKSYIWWMSLGFA 271
           +T   +  F LGI P I +SI+MQ+L  I+P+L KL+K EG  G++KI     +++LG+A
Sbjct: 64  ST---IGIFALGIVPYINSSIVMQLLTKIVPNLEKLQKEEGELGRQKITQITRYLALGWA 123

Query: 272 IVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAMTMTWICDTISESGFGQGSSLII 331
            +++  +S +  P   Y  N     V  +   L  G+M + W+ + I+E G G G+SL+I
Sbjct: 124 TLQSGAISIWVKP---YVFNWNFAFVCESVLALTAGSMIIMWLSELITEKGIGNGASLLI 183

Query: 332 CIGILSGYTETLYKMLSQLS-GRAVIWWPYVLALLGIFTVVTMWAVVVTEGCRKIKLQYY 391
              I+SG  +   +     S   A + +   +A+  +  ++T   + V EG R+IK+   
Sbjct: 184 FQNIVSGLPKNFTQSFFDASYSNASLKFGLFIAIFLLMIIIT---ICVQEGTRRIKI--- 243

Query: 392 GFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYLLAFPSILASVLNSTFWVHVKE 451
              +++     S I +   Y+P  +N  G+ PI+  +  +A PS L  ++ +   + +  
Sbjct: 244 ---ISARQLGKSSILDPNSYLPLKLNQGGVMPIVFASASMALPSYLTQIIQNKTLLQILY 303

Query: 452 ILNPESSIGAEPWVYYLIYAVFVFLFNIF--DIANLPKEISDYLNKMGARIPNIKPGKAT 511
           +  P  S+      Y L+Y   +  F+ F   I   P++I+  L KMGA IPNI+PG+AT
Sbjct: 304 LFCPNGSL------YLLLYCALILFFSYFYTSIVMNPEDIAINLKKMGASIPNIRPGQAT 363

Query: 512 IEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFAIGFTSVLIIVGSIIELRRS 564
           I+YL  I     F G   L  +A    +++   +  N    +G TS+LI+VG  I+  + 
Sbjct: 364 IDYLQVILNRLTFLGASFLFTVALIPFIIEKVTQIQNLR-GLGATSLLILVGVAIDTAKQ 400

BLAST of Cp4.1LG15g00260 vs. NCBI nr
Match: XP_023553756.1 (preprotein translocase subunit SCY2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023553757.1 preprotein translocase subunit SCY2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL
Sbjct: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
           SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD
Sbjct: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
           TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY
Sbjct: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM
Sbjct: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578

BLAST of Cp4.1LG15g00260 vs. NCBI nr
Match: KAG6571476.1 (Preprotein translocase subunit SCY2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1127 bits (2916), Expect = 0.0
Identity = 570/577 (98.79%), Postives = 573/577 (99.31%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           MEAAPLCTRLFPPALFPT PSKFRVAAAYDVQFCPLKFGRSAA+INFNSLKFKGRNNVLL
Sbjct: 1   MEAAPLCTRLFPPALFPTKPSKFRVAAAYDVQFCPLKFGRSAAIINFNSLKFKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
           SRPFV KVNEKSFDCLSNQCR SRMNV ATSEQCLDLETFSQELNDGDAFFTSPNGTNSD
Sbjct: 61  SRPFVPKVNEKSFDCLSNQCRSSRMNVKATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
           TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY
Sbjct: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYS+YAANHRVKHVMVTTFLLVCGAM
Sbjct: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSVYAANHRVKHVMVTTFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIIC+GILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICVGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 577
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 577

BLAST of Cp4.1LG15g00260 vs. NCBI nr
Match: XP_022963692.1 (preprotein translocase subunit SCY2, chloroplastic [Cucurbita moschata] >XP_022963693.1 preprotein translocase subunit SCY2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 570/578 (98.62%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           MEAAPLCTRLFPPALFPT PSKFRVAAAYDVQFCPLKFGRSA+VINFNSLKFKGRNNVLL
Sbjct: 1   MEAAPLCTRLFPPALFPTKPSKFRVAAAYDVQFCPLKFGRSASVINFNSLKFKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
           SRPFVSKVNEKSFDCLSNQCR SRMNV ATSEQCLDLETFSQELNDGDAFFTSPNGTNSD
Sbjct: 61  SRPFVSKVNEKSFDCLSNQCRSSRMNVKATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
           TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY
Sbjct: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYS+YAA+HRVKHVMVTTFLLVCGAM
Sbjct: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSVYAASHRVKHVMVTTFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIIC+GILSGYTETLYKMLSQLSGR+VIWWPYVLALLGIFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICVGILSGYTETLYKMLSQLSGRSVIWWPYVLALLGIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578

BLAST of Cp4.1LG15g00260 vs. NCBI nr
Match: XP_022967504.1 (preprotein translocase subunit SCY2, chloroplastic [Cucurbita maxima] >XP_022967506.1 preprotein translocase subunit SCY2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 561/577 (97.23%), Postives = 564/577 (97.75%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           MEAAP+CTRLFP     T PSKFRVAAAYDVQFCPLKFGRSAAVINFNSLK KGRNNVLL
Sbjct: 1   MEAAPICTRLFP-----TKPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKCKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
           SRPFVSKVNEKSFDCLSNQCR SRMNV ATSEQCLDLETFSQELNDGD FFTSPNGTNS+
Sbjct: 61  SRPFVSKVNEKSFDCLSNQCRSSRMNVKATSEQCLDLETFSQELNDGDVFFTSPNGTNSE 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
           TWKPRSKMFRNRFLNLVRLRS LNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY
Sbjct: 121 TWKPRSKMFRNRFLNLVRLRSFLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM
Sbjct: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIIC+GILSGYTETLYKMLSQLSGR VIWWPYVLALL IFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICVGILSGYTETLYKMLSQLSGRTVIWWPYVLALLAIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 577
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 572

BLAST of Cp4.1LG15g00260 vs. NCBI nr
Match: XP_038888194.1 (preprotein translocase subunit SCY2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1064 bits (2751), Expect = 0.0
Identity = 537/578 (92.91%), Postives = 552/578 (95.50%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           ME  P CTRL  PA FPT PSKF VAA+YDVQFC  KFGRSA VINFN LKFKGRNNVLL
Sbjct: 1   METTPHCTRLLRPAPFPTIPSKFLVAASYDVQFCHFKFGRSATVINFNPLKFKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
           +RP VSKVN+KS DCLSNQC+ SRMNV ATSEQCLDL+TFSQE NDGDAF TSPN TN D
Sbjct: 61  NRPLVSKVNQKSSDCLSNQCQSSRMNVKATSEQCLDLQTFSQESNDGDAFLTSPNATNFD 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
            WKP+SKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISR+GYFIPLPG+
Sbjct: 121 IWKPKSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRVGYFIPLPGF 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDG EKIKSYIWWMSLGFAI+EAL+LSCYSLPYSIYAANHRVKHVMVT+FLLVCGAM
Sbjct: 241 KEGLDGHEKIKSYIWWMSLGFAILEALILSCYSLPYSIYAANHRVKHVMVTSFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIIC+GIL+GYTETLYKMLSQLSG AV WWPYV ALLGIFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICVGILTGYTETLYKMLSQLSGHAVFWWPYVFALLGIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPSILAS+LNSTFW HVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSILASLLNSTFWEHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKA+IEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKASIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578

BLAST of Cp4.1LG15g00260 vs. ExPASy TrEMBL
Match: A0A6J1HFX8 (preprotein translocase subunit SCY2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111463947 PE=3 SV=1)

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 570/578 (98.62%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           MEAAPLCTRLFPPALFPT PSKFRVAAAYDVQFCPLKFGRSA+VINFNSLKFKGRNNVLL
Sbjct: 1   MEAAPLCTRLFPPALFPTKPSKFRVAAAYDVQFCPLKFGRSASVINFNSLKFKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
           SRPFVSKVNEKSFDCLSNQCR SRMNV ATSEQCLDLETFSQELNDGDAFFTSPNGTNSD
Sbjct: 61  SRPFVSKVNEKSFDCLSNQCRSSRMNVKATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
           TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY
Sbjct: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYS+YAA+HRVKHVMVTTFLLVCGAM
Sbjct: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSVYAASHRVKHVMVTTFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIIC+GILSGYTETLYKMLSQLSGR+VIWWPYVLALLGIFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICVGILSGYTETLYKMLSQLSGRSVIWWPYVLALLGIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578

BLAST of Cp4.1LG15g00260 vs. ExPASy TrEMBL
Match: A0A6J1HWW7 (preprotein translocase subunit SCY2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466991 PE=3 SV=1)

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 561/577 (97.23%), Postives = 564/577 (97.75%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           MEAAP+CTRLFP     T PSKFRVAAAYDVQFCPLKFGRSAAVINFNSLK KGRNNVLL
Sbjct: 1   MEAAPICTRLFP-----TKPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKCKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
           SRPFVSKVNEKSFDCLSNQCR SRMNV ATSEQCLDLETFSQELNDGD FFTSPNGTNS+
Sbjct: 61  SRPFVSKVNEKSFDCLSNQCRSSRMNVKATSEQCLDLETFSQELNDGDVFFTSPNGTNSE 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
           TWKPRSKMFRNRFLNLVRLRS LNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY
Sbjct: 121 TWKPRSKMFRNRFLNLVRLRSFLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM
Sbjct: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIIC+GILSGYTETLYKMLSQLSGR VIWWPYVLALL IFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICVGILSGYTETLYKMLSQLSGRTVIWWPYVLALLAIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 577
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 572

BLAST of Cp4.1LG15g00260 vs. ExPASy TrEMBL
Match: A0A1S3BM25 (preprotein translocase subunit SCY2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491333 PE=3 SV=1)

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 527/578 (91.18%), Postives = 549/578 (94.98%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPALFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLL 60
           MEA P CTRL  P L  +  SKFRVAAAYDVQFC  KFGRS  VINFN+ KFKGRNNVLL
Sbjct: 1   MEATPCCTRLLRPPLLHSVHSKFRVAAAYDVQFCHFKFGRSTPVINFNTSKFKGRNNVLL 60

Query: 61  SRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSD 120
            RP VSK+N+KSFDC SNQC+ SRMNV ATSEQCLDLETFSQE NDGDAF TS NGTN D
Sbjct: 61  KRPLVSKMNQKSFDCFSNQCQNSRMNVKATSEQCLDLETFSQESNDGDAFLTSSNGTNFD 120

Query: 121 TWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGY 180
            WKP+S+ FRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISR+GYFIPLPG+
Sbjct: 121 IWKPKSRAFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRVGYFIPLPGF 180

Query: 181 DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240
           DRRLIPQDYLSFVSGS DELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR
Sbjct: 181 DRRLIPQDYLSFVSGSADELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLR 240

Query: 241 KEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAM 300
           KEGLDG EKIKSYIWWMSLGFAI+EALVLSCYSLPYSIYAANHRVKHVMVT+FLLVCGAM
Sbjct: 241 KEGLDGHEKIKSYIWWMSLGFAILEALVLSCYSLPYSIYAANHRVKHVMVTSFLLVCGAM 300

Query: 301 TMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFT 360
           TMTWICDTISESGFGQGSSLIIC+GIL+GYTETLYKMLSQLSGRAV+WWPYVLALLGIFT
Sbjct: 301 TMTWICDTISESGFGQGSSLIICVGILTGYTETLYKMLSQLSGRAVVWWPYVLALLGIFT 360

Query: 361 VVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420
           +VTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL
Sbjct: 361 IVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYL 420

Query: 421 LAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEIS 480
           LAFPS+LAS+L STFW HVKEILNPESSIGAEPWV+YL+YAVFVFLFNIFDIANLPKEIS
Sbjct: 421 LAFPSVLASLLKSTFWEHVKEILNPESSIGAEPWVFYLLYAVFVFLFNIFDIANLPKEIS 480

Query: 481 DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFA 540
           DYLNKMGARIPNIKPGKA+IEYLTK+QASTRFWGGLLLSFLATTSSLLDHYLRS+NAGFA
Sbjct: 481 DYLNKMGARIPNIKPGKASIEYLTKVQASTRFWGGLLLSFLATTSSLLDHYLRSVNAGFA 540

Query: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578
           IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV
Sbjct: 541 IGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578

BLAST of Cp4.1LG15g00260 vs. ExPASy TrEMBL
Match: A0A6J1DS34 (preprotein translocase subunit SCY2, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022689 PE=3 SV=1)

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 514/580 (88.62%), Postives = 539/580 (92.93%), Query Frame = 0

Query: 1   MEAAPLCTRLFPPA--LFPTTPSKFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNV 60
           MEA    TRL  P   LFPT  SKFRVA AYDV+FC L+FGRS+ VIN  SL+FKGR + 
Sbjct: 1   MEATHHSTRLLHPIPILFPTRTSKFRVAVAYDVRFCRLQFGRSSTVINSKSLEFKGRTHX 60

Query: 61  LLSRPFVSKVNEKSFDCLSNQCRRSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTN 120
           L +R F  KVN+KSF+CLSNQC+ S +NV ATSEQCLDLETF QE  DGDAF TSPNG++
Sbjct: 61  LSNRHFAYKVNQKSFECLSNQCQSSHLNVKATSEQCLDLETFPQESQDGDAFLTSPNGSD 120

Query: 121 SDTWKPRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLP 180
            DTWKPRS+ F NRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISR+GYFIPLP
Sbjct: 121 FDTWKPRSQKFMNRFLNLVRLRSVLNNAAESFFKSEIRRRLFVTAVLIVISRVGYFIPLP 180

Query: 181 GYDRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVK 240
           G+DRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHI+PSLVK
Sbjct: 181 GFDRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGISPQIIASIIMQVLCHIVPSLVK 240

Query: 241 LRKEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCG 300
           LRKEGLDG EKIKSYIWWMSLGFAI+EAL+LSCYSLPYSIYAANHRVKHVMVT+ LLVCG
Sbjct: 241 LRKEGLDGHEKIKSYIWWMSLGFAILEALILSCYSLPYSIYAANHRVKHVMVTSLLLVCG 300

Query: 301 AMTMTWICDTISESGFGQGSSLIICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGI 360
           AMTMTWICDTISESGFG GSSLIIC+GIL+GYTETLYKMLSQLSGRAVIW PYVL LLGI
Sbjct: 301 AMTMTWICDTISESGFGXGSSLIICVGILTGYTETLYKMLSQLSGRAVIWLPYVLTLLGI 360

Query: 361 FTVVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTT 420
           FT+VTMWAVVVTEGCRKIKLQYYGFKLASAAR+DSPITEVEPYIPFNINPAGMQPILTTT
Sbjct: 361 FTIVTMWAVVVTEGCRKIKLQYYGFKLASAARDDSPITEVEPYIPFNINPAGMQPILTTT 420

Query: 421 YLLAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKE 480
           YLLA PSILAS+LNSTFW HVKEILNPESSIGAEPWVYY IYAVFVFLFNIFDIANLPKE
Sbjct: 421 YLLASPSILASLLNSTFWEHVKEILNPESSIGAEPWVYYSIYAVFVFLFNIFDIANLPKE 480

Query: 481 ISDYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAG 540
           ISDYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLS LATTSS+LDHYLRSINAG
Sbjct: 481 ISDYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLLLSILATTSSVLDHYLRSINAG 540

Query: 541 FAIGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 578
           FAIGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV
Sbjct: 541 FAIGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYGV 580

BLAST of Cp4.1LG15g00260 vs. ExPASy TrEMBL
Match: A0A6J1DMV0 (preprotein translocase subunit SCY2, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022689 PE=3 SV=1)

HSP 1 Score: 991 bits (2563), Expect = 0.0
Identity = 500/557 (89.77%), Postives = 527/557 (94.61%), Query Frame = 0

Query: 22  KFRVAAAYDVQFCPLKFGRSAAVINFNSLKFKGRNNVLLSRPFVSKVNEKSFDCLSNQCR 81
           K++VA AYDV+FC L+FGRS+ VIN  SL+FKGR + L +R F  KVN+KSF+CLSNQC+
Sbjct: 30  KWKVAVAYDVRFCRLQFGRSSTVINSKSLEFKGRTHXLSNRHFAYKVNQKSFECLSNQCQ 89

Query: 82  RSRMNVNATSEQCLDLETFSQELNDGDAFFTSPNGTNSDTWKPRSKMFRNRFLNLVRLRS 141
            S +NV ATSEQCLDLETF QE  DGDAF TSPNG++ DTWKPRS+ F NRFLNLVRLRS
Sbjct: 90  SSHLNVKATSEQCLDLETFPQESQDGDAFLTSPNGSDFDTWKPRSQKFMNRFLNLVRLRS 149

Query: 142 VLNNAAESFFKSEIRRRLFVTAVLIVISRIGYFIPLPGYDRRLIPQDYLSFVSGSVDELG 201
           VLNNAAESFFKSEIRRRLFVTAVLIVISR+GYFIPLPG+DRRLIPQDYLSFVSGSVDELG
Sbjct: 150 VLNNAAESFFKSEIRRRLFVTAVLIVISRVGYFIPLPGFDRRLIPQDYLSFVSGSVDELG 209

Query: 202 DFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKLRKEGLDGQEKIKSYIWWMSLGF 261
           DFTTEMKMSFFQLGISPQIIASIIMQVLCHI+PSLVKLRKEGLDG EKIKSYIWWMSLGF
Sbjct: 210 DFTTEMKMSFFQLGISPQIIASIIMQVLCHIVPSLVKLRKEGLDGHEKIKSYIWWMSLGF 269

Query: 262 AIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAMTMTWICDTISESGFGQGSSLI 321
           AI+EAL+LSCYSLPYSIYAANHRVKHVMVT+ LLVCGAMTMTWICDTISESGFG GSSLI
Sbjct: 270 AILEALILSCYSLPYSIYAANHRVKHVMVTSLLLVCGAMTMTWICDTISESGFGXGSSLI 329

Query: 322 ICIGILSGYTETLYKMLSQLSGRAVIWWPYVLALLGIFTVVTMWAVVVTEGCRKIKLQYY 381
           IC+GIL+GYTETLYKMLSQLSGRAVIW PYVL LLGIFT+VTMWAVVVTEGCRKIKLQYY
Sbjct: 330 ICVGILTGYTETLYKMLSQLSGRAVIWLPYVLTLLGIFTIVTMWAVVVTEGCRKIKLQYY 389

Query: 382 GFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYLLAFPSILASVLNSTFWVHVKE 441
           GFKLASAAR+DSPITEVEPYIPFNINPAGMQPILTTTYLLA PSILAS+LNSTFW HVKE
Sbjct: 390 GFKLASAARDDSPITEVEPYIPFNINPAGMQPILTTTYLLASPSILASLLNSTFWEHVKE 449

Query: 442 ILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEISDYLNKMGARIPNIKPGKATIE 501
           ILNPESSIGAEPWVYY IYAVFVFLFNIFDIANLPKEISDYLNKMGARIPNIKPGKATIE
Sbjct: 450 ILNPESSIGAEPWVYYSIYAVFVFLFNIFDIANLPKEISDYLNKMGARIPNIKPGKATIE 509

Query: 502 YLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFAIGFTSVLIIVGSIIELRRSYQ 561
           YLTKIQASTRFWGGLLLS LATTSS+LDHYLRSINAGFAIGFTSVLIIVGSIIELRRSYQ
Sbjct: 510 YLTKIQASTRFWGGLLLSILATTSSVLDHYLRSINAGFAIGFTSVLIIVGSIIELRRSYQ 569

Query: 562 AYNVMPSLSNALRRYGV 578
           AYNVMPSLSNALRRYGV
Sbjct: 570 AYNVMPSLSNALRRYGV 586

BLAST of Cp4.1LG15g00260 vs. TAIR 10
Match: AT2G31530.1 (SecY protein transport family protein )

HSP 1 Score: 758.8 bits (1958), Expect = 3.1e-219
Identity = 395/541 (73.01%), Postives = 451/541 (83.36%), Query Frame = 0

Query: 40  RSAAVINFNSLKFKGRNNVLLSRPFVSKVNEKSFDC-LSNQCRRSRMNVNATSEQCLDLE 99
           R     N N  + +  +++ ++R  +     KSF    S++ R   M+        LD+E
Sbjct: 38  RKCLTTNLNMSRTRQGHSIQMNRRHLLMKERKSFSINYSDKFRDDSMSSEEMHTDALDVE 97

Query: 100 TFSQELNDGDAFFTSPNGTNSDTWK-PRSKMFRNRFLNLVRLRSVLNNAAESFFKSEIRR 159
               +  D      S N   S+T +  R K FRNRFL+ VR+ SVLN AAE FFKSEIRR
Sbjct: 98  IIPPDSQD---IRNSQNSAVSNTLQDDRPKSFRNRFLDFVRISSVLNTAAERFFKSEIRR 157

Query: 160 RLFVTAVLIVISRIGYFIPLPGYDRRLIPQDYLSFVSGSVDELGDFTTEMKMSFFQLGIS 219
           RLFVTAVL+V+SR+GYFIPLPG+DRRLIPQDYLSFVSGSV+ELG+F  E+K+S FQLG+S
Sbjct: 158 RLFVTAVLLVLSRVGYFIPLPGFDRRLIPQDYLSFVSGSVEELGEFGAEIKLSLFQLGLS 217

Query: 220 PQIIASIIMQVLCHILPSLVKLRKEGLDGQEKIKSYIWWMSLGFAIVEALVLSCYSLPYS 279
           PQIIASIIMQVLCH+LPSLVKLRKEGLDG EKIKSYIWW+S  FAIVEALV++  SL YS
Sbjct: 218 PQIIASIIMQVLCHVLPSLVKLRKEGLDGHEKIKSYIWWLSFFFAIVEALVVAYTSLQYS 277

Query: 280 IYAANHRVKHVMVTTFLLVCGAMTMTWICDTISESGFGQGSSLIICIGILSGYTETLYKM 339
           ++AA  +VKHVM+T+ LLVCGAMTMTW+CDTISESGFG GSSLIIC+GIL+GYTETL+KM
Sbjct: 278 VFAATAQVKHVMMTSSLLVCGAMTMTWLCDTISESGFGHGSSLIICVGILTGYTETLHKM 337

Query: 340 LSQLSGRAVIWWPYVLALLGIFTVVTMWAVVVTEGCRKIKLQYYGFKLASAAREDSPITE 399
           L+Q+SG    W PY+L LLGIFTVVTM+AVVVTEGCRKIKLQYYGFKLASA+RE SPITE
Sbjct: 338 LNQISGSFSNWLPYLLGLLGIFTVVTMFAVVVTEGCRKIKLQYYGFKLASASREGSPITE 397

Query: 400 VEPYIPFNINPAGMQPILTTTYLLAFPSILASVLNSTFWVHVKEILNPESSIGAEPWVYY 459
           VEPYIPFNINPAGMQP+LTTTYLLAFPSILAS+L S F +++KEILNPES++GA PWVYY
Sbjct: 398 VEPYIPFNINPAGMQPVLTTTYLLAFPSILASILGSPFLLNMKEILNPESTVGAPPWVYY 457

Query: 460 LIYAVFVFLFNIFDIANLPKEISDYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLL 519
            IYA FVFLFNIFDIANLPKEI+DYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLL
Sbjct: 458 SIYAFFVFLFNIFDIANLPKEIADYLNKMGARIPNIKPGKATIEYLTKIQASTRFWGGLL 517

Query: 520 LSFLATTSSLLDHYLRSINAGFAIGFTSVLIIVGSIIELRRSYQAYNVMPSLSNALRRYG 579
           LSFLAT S++LDHYLRSIN GF+IGFTSVLIIVGSIIELRRSY AYNVMPSLS AL+RYG
Sbjct: 518 LSFLATASTVLDHYLRSINQGFSIGFTSVLIIVGSIIELRRSYHAYNVMPSLSKALKRYG 575

BLAST of Cp4.1LG15g00260 vs. TAIR 10
Match: AT2G18710.1 (SECY homolog 1 )

HSP 1 Score: 138.3 bits (347), Expect = 2.0e-32
Identity = 125/438 (28.54%), Postives = 198/438 (45.21%), Query Frame = 0

Query: 150 FFKSEIRRRLFVTAVLIVISRIGYFIPLPGYDRRL----IPQDYL-----SFVSGSVDEL 209
           FFK  +  +       + +SR+G +IPL G +R      + Q+ +     +F  G +  L
Sbjct: 133 FFKGPLPGKFLKLLGFLALSRLGIYIPLGGVNREAFVGNLDQNSILSTLDTFSGGGIGRL 192

Query: 210 GDFTTEMKMSFFQLGISPQIIASIIMQVLCHILPSLVKL-RKEGLDGQEKIKSYIWWMSL 269
           G            LGI P I A I+ Q+L  + P L  L +KEG  G++KI  Y  + S+
Sbjct: 193 G---------ICSLGIVPFINAQIVFQLLAQVYPKLQDLQKKEGEAGRKKILQYTRYASV 252

Query: 270 GFAIVEALVLSCYSLPYSIYAANHRVKHVMVTTFLLVCGAMTMTWICDTISESGFGQGSS 329
           GFAIV+A+    Y  P   Y  +   + V+ +  LL  G++  T+I + IS+   G G+S
Sbjct: 253 GFAIVQAIGQVFYLRP---YVNDFSTEWVVSSVTLLTLGSVLTTYIGERISDLKLGNGTS 312

Query: 330 LIICIGILS------GYTETLYKMLSQLSGRAVIWWPYVLALLGIFTVVTMWAVVVTEGC 389
           L+I   I+S      G T          +G   I   ++L +LGI        V V E  
Sbjct: 313 LLIFTSIISYLPASFGRTTAEALQEGNYTGLGTIVVSFLLLVLGI--------VYVQEAE 372

Query: 390 RKIKLQYYGFKLASAAREDSPITEVEPYIPFNINPAGMQPILTTTYLLAFPSILASVLNS 449
           RKI L Y     AS     +   +   Y+PF +N AG+ PI+ +T  LA P+ LA     
Sbjct: 373 RKIPLNY-----ASRYTSKAGGLQKSAYLPFKVNSAGVMPIIFSTSSLALPATLARFTGI 432

Query: 450 TFWVHVKEILNPESSIGAEPWVYYLIYAVFVFLFNIFDIANLPKEISDYLNKMGARIPNI 509
           +   +V   L P  S      +  + +  + + F   D    P ++S+ L + GA IP +
Sbjct: 433 SALKNVAFALTPGGSFYLPTNILLIAFFNYYYTFLQLD----PDDVSEQLKRQGASIPLV 492

Query: 510 KPGKATIEYLTKIQASTRFWGGLLLSFLATTSSLLDHYLRSINAGFAIGFTSVLIIVGSI 569
           +PGK+T  ++  +       G   L+ LA   ++++  +  + A      TSVLI+VG  
Sbjct: 493 RPGKSTALFIKTVLGRISVLGSAFLAVLAAGPAVVEQ-ITHLTAFRGFAGTSVLILVGCA 540

Query: 570 IELRRSYQAYNVMPSLSN 572
            +  R  QA  +     N
Sbjct: 553 TDTARKVQAEIISQKYKN 540