Cp4.1LG09g07150 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g07150 vs. ExPASy Swiss-Prot
Match: Q84MA5 (Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 1.6e-233
Identity = 403/567 (71.08%), Postives = 471/567 (83.07%), Query Frame = 0

Query: 5   EEGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARS 64
           ++G+RRR C C+K+DFLPEESF+S  NY KALK TP+R +DR+  RS +  ++ EMKARS
Sbjct: 7   DDGLRRRGCSCTKDDFLPEESFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINEMKARS 66

Query: 65  QHEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCY 124
            HEMKK+LTWWDLMWFGIGAVIG+GIFVLTGLE + H+GPA+VLSYVVSGVSAMLSVFCY
Sbjct: 67  GHEMKKTLTWWDLMWFGIGAVIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCY 126

Query: 125 TEFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHP 184
           TEFAVEIP AGGSFAYLRVELGDFMAFI AGNI+LEYV+GGAAVARSWTSYFATL NH P
Sbjct: 127 TEFAVEIPVAGGSFAYLRVELGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATLLNHKP 186

Query: 185 NDFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIA 244
            DFRI V    +DY+ LDPIA+ V  +IC LAV  TKGSSRFNYIASI H+V+ILF+IIA
Sbjct: 187 EDFRIIVHKLGEDYSHLDPIAVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIA 246

Query: 245 GLTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSM 304
           G TKA+ KN++ F PYG RG+F ++AVLFFAY+GFDAVSTMAEETKNP +DIPIGLVGSM
Sbjct: 247 GFTKADVKNYSDFTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSM 306

Query: 305 VITT------XVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVS 364
           V+TT       VTLCLMQPYQQID DAPFSVAF AVGW WAKYIVA GA+KGMTTVLLV 
Sbjct: 307 VVTTVCYCLMAVTLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVG 366

Query: 365 AVGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSI 424
           A+GQARY+THIAR HM+ PW A+V+ +T  P+NAT  MLAATA+IAFFT L IL++LLS+
Sbjct: 367 AIGQARYMTHIARAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSV 426

Query: 425 STLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWS-SSDGWIGL 484
           STLFIFM VAV L+VRRYYV+GET+  DRNK ++ + LIL SS ATAVYW+   +GWIG 
Sbjct: 427 STLFIFMFVAVALLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEEGWIGY 486

Query: 485 VVSNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGL 544
            ++  IWFLST+ +   VPQA+ P++WGVPLVPW+PS SIAIN+FLLGSID  SF RF +
Sbjct: 487 CITVPIWFLSTVAMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAI 546

Query: 545 WTGILLIYYFLIGLHASYDSVMESKTE 565
           WTGILLIYY L GLHA+YD+   +  E
Sbjct: 547 WTGILLIYYVLFGLHATYDTAKATLKE 573

BLAST of Cp4.1LG09g07150 vs. ExPASy Swiss-Prot
Match: O64759 (Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 1.7e-177
Identity = 322/559 (57.60%), Postives = 412/559 (73.70%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           EG  R     SK DF PEESF+S+ +Y  AL  T +R  +RL +RS +  +  E+K +S+
Sbjct: 2   EGEERGYWRWSKRDFFPEESFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSE 61

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMK+ LTWWDL+WFG G+VIGAGIFVLTG E  + AGPAIVLSYVVSG+SAMLSVFCYT
Sbjct: 62  HEMKRCLTWWDLVWFGFGSVIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYT 121

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIP AGGSFAYLR+ELGDF AFITAGNILLE ++G AAVAR+WTSYFATL N  PN
Sbjct: 122 EFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPN 181

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
             RI  +     +N LDPIA+VVI    T+A  ST+ +S  N+IAS  + ++I F+IIAG
Sbjct: 182 ALRIK-TDLSSGFNLLDPIAVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAG 241

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
              A+  N TPF P+GP G+F A+AV++FAY GFD+++TMAEETKNP++DIPIGL+GSM 
Sbjct: 242 FIHADTSNLTPFLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMS 301

Query: 306 ITTXV------TLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           I T +      +L +MQ Y  ID +A +SVAF++VG  W KY+VA+GA+KGMTTVLLV A
Sbjct: 302 IITVIYCLMALSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGA 361

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           +GQARY+THIARTHMI P FA VH +T  P+NA   +   +A+IAFF+ L +L++LLSIS
Sbjct: 362 LGQARYVTHIARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSIS 421

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYW--SSSDGWIGL 485
           TLFIF ++ + L+VRRYYV  +T      KLI C++ ++ SS+ T+ YW       WIG 
Sbjct: 422 TLFIFTMMPIALLVRRYYVRQDTPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGY 481

Query: 486 VVSNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGL 545
            V+   WFL TL +   VPQ + P+VWGVPLVPW+P LSIA N+FL+GS+   +F RFG+
Sbjct: 482 TVTVPFWFLGTLGIVFFVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGV 541

Query: 546 WTGILLIYYFLIGLHASYD 557
            T  +L+YYFL+GLHA++D
Sbjct: 542 CTLAMLLYYFLLGLHATFD 559

BLAST of Cp4.1LG09g07150 vs. ExPASy Swiss-Prot
Match: Q9SHH0 (Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=CAT8 PE=2 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 8.0e-156
Identity = 285/556 (51.26%), Postives = 388/556 (69.78%), Query Frame = 0

Query: 17  KEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQHEMKKSLTWWD 76
           K+DF PE SF+S+  Y  AL AT  RL DRL +RS +  +L   +  S++ M++ LTWWD
Sbjct: 30  KQDFFPEFSFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWD 89

Query: 77  LMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYTEFAVEIPAAGG 136
           L+W   G+V+G+G+FV+TG E +  AGPA+VLSY +SGVSA+LSV CY EF VEIP AGG
Sbjct: 90  LLWLSFGSVVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGG 149

Query: 137 SFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPNDFRIHVSSFPD 196
           SF+YLRVELGDF+AFI AGNILLE ++G A + RSW+SY A+L  +  + FRI V SF  
Sbjct: 150 SFSYLRVELGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYFRIKVDSFAK 209

Query: 197 DYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAGLTKANPKNFTP 256
            ++ LDP+A+ V++V   +A+  TK +S  N I S+  V II+FI++ G T +   N  P
Sbjct: 210 GFDLLDPVAVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP 269

Query: 257 FAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMVITTXV------ 316
           F PYG +G+  ++AV++++Y GFD V+ MAEET+ P++DIPIGLVGSM + T V      
Sbjct: 270 FFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMAL 329

Query: 317 TLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSAVGQARYLTHIA 376
            L +M  Y +ID +A +SVAF  +G  WAKY+V + A+KGMTT LLV ++GQARY T IA
Sbjct: 330 ALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIA 389

Query: 377 RTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSISTLFIFMLVAVG 436
           R+HMI PWFA VH +T  P+ AT  +   ++II+FFTSL +LS++ S +TLFIFMLVAV 
Sbjct: 390 RSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAVA 449

Query: 437 LIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSS-DGWIGLVVSNSIWFLSTL 496
           L+VRRYYV   T  +   K +  + LI+ SSI  +  W+S   GWI   V+  IWF+ TL
Sbjct: 450 LLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVTGVIWFIGTL 509

Query: 497 VLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWTGILLIYYFLI 556
            L L +P+ + P+VWGVPLVPW+PS SIA+NLFL+GS+   +F RF + T ++L+YY  +
Sbjct: 510 GLAL-LPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLYYLFV 569

Query: 557 GLHASYDSVMESKTEA 566
           GLHA+YD   +   EA
Sbjct: 570 GLHATYDVAHQPLEEA 584

BLAST of Cp4.1LG09g07150 vs. ExPASy Swiss-Prot
Match: Q9LZ20 (Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT6 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 8.4e-121
Identity = 232/552 (42.03%), Postives = 350/552 (63.41%), Query Frame = 0

Query: 25  SFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQHEMKKSLTWWDLMWFGIGA 84
           SF S R Y  +L ATP+RL  R  + S    ++  ++A S  +M+++L W+DL+  GIG 
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 85  VIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYTEFAVEIPAAGGSFAYLRVE 144
           ++GAG+FV TG  ++  AGP+IV+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R+ 
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 145 LGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPNDFRIHVSSFPDDYNQLDPI 204
            G+F AF T  N++++YV+  AAV+RS+T+Y  T      + +R  VS  P  +N++DP+
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDPV 193

Query: 205 AIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAGLTK---------ANPKNFT 264
           A++V++VI  +   ST+ SS+ N I + FH+  I F+I+ G  K         ANP++ +
Sbjct: 194 AVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPS 253

Query: 265 PFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMVITT------X 324
            F P+G  G+F  +A+++ +Y+G+DAVSTMAEE +NP KDIP+G+ GS+ I T       
Sbjct: 254 GFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMA 313

Query: 325 VTLCLMQPYQQIDEDAPFSVAFE-AVGWGWAKYIVAVGAIKGMTTVLLVSAVGQARYLTH 384
           V++ ++ PY  ID +APFS AF  + GW W   +V +GA  G+ T LLV+ +GQARY+  
Sbjct: 314 VSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCV 373

Query: 385 IARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSISTLFIFMLVA 444
           I R+ ++  WFAK+H +TS PVNA+  +   TA +A FT L +L NL+SI TLF+F +VA
Sbjct: 374 IGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVA 433

Query: 445 VGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDG---WIGLVVSNSIWF 504
             LI RRY   G T P     L    +  + S + T ++    +G      L  S  +  
Sbjct: 434 NALIFRRYVPVGPTKPWP--TLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVAI 493

Query: 505 LSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWTGILLIY 558
              L     VPQA+KP +WGVP +PW P +SI +N+FLLGS+D  S+ RFG ++G++++ 
Sbjct: 494 AIVLSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVLV 553

BLAST of Cp4.1LG09g07150 vs. ExPASy Swiss-Prot
Match: Q9SQZ0 (Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT7 PE=3 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.2e-120
Identity = 226/555 (40.72%), Postives = 353/555 (63.60%), Query Frame = 0

Query: 23  EESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQHEMKKSLTWWDLMWFGI 82
           + SF S R Y  +L  TP+R   R  + S  + ++  ++A S  +M+++L W+DL+  GI
Sbjct: 11  DTSFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGI 70

Query: 83  GAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYTEFAVEIPAAGGSFAYLR 142
           G +IGAG+FV TG  ++ +AGP+IV+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R
Sbjct: 71  GGMIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIR 130

Query: 143 VELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPNDFRIHVSSFPDDYNQLD 202
           +  G+F AFIT  N++++YV+  AAV+R +T+Y  +      +++R  VS  P+ +N++D
Sbjct: 131 ITFGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEID 190

Query: 203 PIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAGLTKA---------NPKN 262
           PIA++V++ +  +   ST+ SS+ N + +  H+  I+F+I+ G +K          NP+N
Sbjct: 191 PIAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPEN 250

Query: 263 FTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMVIT------ 322
            + F P+G  G+F  +A+++ +Y+G+DAVSTMAEE K+P KDIP+G+ GS+ I       
Sbjct: 251 PSGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCL 310

Query: 323 TXVTLCLMQPYQQIDEDAPFSVAF-EAVGWGWAKYIVAVGAIKGMTTVLLVSAVGQARYL 382
             +++ ++ PY  ID +AP+S AF ++ GW W   +V +GA  G+ T L+V+ +GQARY+
Sbjct: 311 MAISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYM 370

Query: 383 THIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSISTLFIFML 442
             I R+ ++  WFAKVH +TS PVNA+A +   TA++A FT L +L NL+SI TLF+F +
Sbjct: 371 CVIGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYM 430

Query: 443 VAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYW----SSSDGWIGLVVSNS 502
           VA  +I RRY   G T P       +C+  I  +SI   + W    S    W  L  S  
Sbjct: 431 VANAVIFRRYVTVGYTEPWP-TLSFLCLFSI--TSILFTLVWQLAPSGPPKWFILGASTV 490

Query: 503 IWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWTGIL 558
                  +    VPQA+ P  WGVPL+PW P +SI +N+FLLGS+D  S+ RFG ++G++
Sbjct: 491 TAIAIVQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLV 550

BLAST of Cp4.1LG09g07150 vs. NCBI nr
Match: XP_022994933.1 (cationic amino acid transporter 1-like [Cucurbita maxima])

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 540/573 (94.24%), Postives = 551/573 (96.16%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           EGIRRRSC CSKEDFLPEESFKSW NY+KALKATP RLLDRLTARSGEHT+LVEMKARSQ
Sbjct: 12  EGIRRRSCSCSKEDFLPEESFKSWGNYTKALKATPARLLDRLTARSGEHTELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVII ICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLV+A
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVNA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGETTPSDRNKL+IC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLVICIVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNNRV 572
           GILL+YYFLIGLHASYDSVMESKT A  VN+ V
Sbjct: 552 GILLVYYFLIGLHASYDSVMESKTVAGEVNSSV 584

BLAST of Cp4.1LG09g07150 vs. NCBI nr
Match: XP_022994771.1 (cationic amino acid transporter 1-like [Cucurbita maxima])

HSP 1 Score: 1064 bits (2751), Expect = 0.0
Identity = 540/573 (94.24%), Postives = 551/573 (96.16%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           E IRRRSCGCSK+DFLPEESFKSW NY++ALKATP RLLDRLTARSGEHT+LVEMKARSQ
Sbjct: 12  EEIRRRSCGCSKQDFLPEESFKSWGNYTEALKATPARLLDRLTARSGEHTELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVII ICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIIC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICIVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNNRV 572
           GILL+YYFLIGLHASYDSVMESKT A  VN+ V
Sbjct: 552 GILLVYYFLIGLHASYDSVMESKTVAGEVNSSV 584

BLAST of Cp4.1LG09g07150 vs. NCBI nr
Match: XP_022994106.1 (cationic amino acid transporter 1-like [Cucurbita maxima])

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 537/573 (93.72%), Postives = 549/573 (95.81%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           E IRRRSC CSKEDFLPEESFKSW NY+KALKATP RLLDRLTARSGEHT+LVEMKA+SQ
Sbjct: 12  EEIRRRSCSCSKEDFLPEESFKSWGNYTKALKATPARLLDRLTARSGEHTELVEMKAQSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKH GPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHVGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVII ICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGETTPSDRNKL+IC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLVICIVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNNRV 572
           GILL+YYFLIGLHASYDS+MESKT A  VN+ V
Sbjct: 552 GILLVYYFLIGLHASYDSIMESKTVAGEVNSSV 584

BLAST of Cp4.1LG09g07150 vs. NCBI nr
Match: XP_022955317.1 (cationic amino acid transporter 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 537/571 (94.05%), Postives = 547/571 (95.80%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           EGIRRRSC CSKEDFLPEESFKSW NY+KALKATP R LDRLTARSGEHT+LVEMKARSQ
Sbjct: 12  EGIRRRSCSCSKEDFLPEESFKSWGNYTKALKATPARFLDRLTARSGEHTELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGET PSDRNKLIIC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETIPSDRNKLIICLVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKP VWGVPLVPW+PSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPHVWGVPLVPWLPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNN 570
           GILL+YY LIGLHASYDSVMESKT A  VN+
Sbjct: 552 GILLVYYILIGLHASYDSVMESKTIAGEVNS 582

BLAST of Cp4.1LG09g07150 vs. NCBI nr
Match: KAG6573394.1 (Cationic amino acid transporter 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 535/571 (93.70%), Postives = 548/571 (95.97%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           EGIRRRSCGCSKEDFLPEESFKSW NY+KALKATP RLLDRLTARSGEHT+LVEMKARSQ
Sbjct: 12  EGIRRRSCGCSKEDFLPEESFKSWGNYTKALKATPARLLDRLTARSGEHTELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVI AGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVISAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVS+FPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSTFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIIC+VLILGSSIATAVYW SS+GWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICLVLILGSSIATAVYWGSSNGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKPRVWGVPLVPW+PSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWLPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNN 570
           GIL +YY LIGLHASYDS +ESKT A  VN+
Sbjct: 552 GILFVYYILIGLHASYDSAIESKTVAGEVNS 582

BLAST of Cp4.1LG09g07150 vs. ExPASy TrEMBL
Match: A0A6J1K6G4 (cationic amino acid transporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490510 PE=4 SV=1)

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 540/573 (94.24%), Postives = 551/573 (96.16%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           EGIRRRSC CSKEDFLPEESFKSW NY+KALKATP RLLDRLTARSGEHT+LVEMKARSQ
Sbjct: 12  EGIRRRSCSCSKEDFLPEESFKSWGNYTKALKATPARLLDRLTARSGEHTELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVII ICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLV+A
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVNA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGETTPSDRNKL+IC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLVICIVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNNRV 572
           GILL+YYFLIGLHASYDSVMESKT A  VN+ V
Sbjct: 552 GILLVYYFLIGLHASYDSVMESKTVAGEVNSSV 584

BLAST of Cp4.1LG09g07150 vs. ExPASy TrEMBL
Match: A0A6J1K040 (cationic amino acid transporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490398 PE=4 SV=1)

HSP 1 Score: 1064 bits (2751), Expect = 0.0
Identity = 540/573 (94.24%), Postives = 551/573 (96.16%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           E IRRRSCGCSK+DFLPEESFKSW NY++ALKATP RLLDRLTARSGEHT+LVEMKARSQ
Sbjct: 12  EEIRRRSCGCSKQDFLPEESFKSWGNYTEALKATPARLLDRLTARSGEHTELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVII ICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIIC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICIVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNNRV 572
           GILL+YYFLIGLHASYDSVMESKT A  VN+ V
Sbjct: 552 GILLVYYFLIGLHASYDSVMESKTVAGEVNSSV 584

BLAST of Cp4.1LG09g07150 vs. ExPASy TrEMBL
Match: A0A6J1JY64 (cationic amino acid transporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111489934 PE=4 SV=1)

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 537/573 (93.72%), Postives = 549/573 (95.81%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           E IRRRSC CSKEDFLPEESFKSW NY+KALKATP RLLDRLTARSGEHT+LVEMKA+SQ
Sbjct: 12  EEIRRRSCSCSKEDFLPEESFKSWGNYTKALKATPARLLDRLTARSGEHTELVEMKAQSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKH GPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHVGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVII ICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIAICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGETTPSDRNKL+IC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLVICIVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNNRV 572
           GILL+YYFLIGLHASYDS+MESKT A  VN+ V
Sbjct: 552 GILLVYYFLIGLHASYDSIMESKTVAGEVNSSV 584

BLAST of Cp4.1LG09g07150 vs. ExPASy TrEMBL
Match: A0A6J1GT83 (cationic amino acid transporter 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111457315 PE=4 SV=1)

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 537/571 (94.05%), Postives = 547/571 (95.80%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           EGIRRRSC CSKEDFLPEESFKSW NY+KALKATP R LDRLTARSGEHT+LVEMKARSQ
Sbjct: 12  EGIRRRSCSCSKEDFLPEESFKSWGNYTKALKATPARFLDRLTARSGEHTELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGET PSDRNKLIIC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETIPSDRNKLIICLVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKP VWGVPLVPW+PSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPHVWGVPLVPWLPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNN 570
           GILL+YY LIGLHASYDSVMESKT A  VN+
Sbjct: 552 GILLVYYILIGLHASYDSVMESKTIAGEVNS 582

BLAST of Cp4.1LG09g07150 vs. ExPASy TrEMBL
Match: A0A6J1GTG0 (cationic amino acid transporter 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457315 PE=4 SV=1)

HSP 1 Score: 1053 bits (2724), Expect = 0.0
Identity = 534/571 (93.52%), Postives = 547/571 (95.80%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           E IRRRSCGCSK+DFLPEESFKSW NY++ALKATP RLLDRLTARSG+H +LVEMKARSQ
Sbjct: 12  EEIRRRSCGCSKQDFLPEESFKSWGNYTRALKATPARLLDRLTARSGDHIELVEMKARSQ 71

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPA+VLSYVVSGVSAMLSVFCYT
Sbjct: 72  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAVVLSYVVSGVSAMLSVFCYT 131

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIPAAGGSFAYLRVELGDF+AFI AGNILLEYVIGGAAVARSWTSYFATLCNHHPN
Sbjct: 132 EFAVEIPAAGGSFAYLRVELGDFVAFIAAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 191

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
           DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHV IILFI+IAG
Sbjct: 192 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVAIILFIVIAG 251

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
           LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV
Sbjct: 252 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 311

Query: 306 ITTX------VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           ITT       VTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVA GAIKGMTTVLLVSA
Sbjct: 312 ITTLAYCILAVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAAGAIKGMTTVLLVSA 371

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           VGQARYLTHIARTHMISPWFAKVHERT  PVNATATMLAATAIIAFFTSLGILSNLLSIS
Sbjct: 372 VGQARYLTHIARTHMISPWFAKVHERTGTPVNATATMLAATAIIAFFTSLGILSNLLSIS 431

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDGWIGLVV 485
           TLFIFMLVAVGLIVRRYYVSGET PSDRNKLIIC+VLILGSSIATAVYW SSDGWIGLVV
Sbjct: 432 TLFIFMLVAVGLIVRRYYVSGETIPSDRNKLIICLVLILGSSIATAVYWGSSDGWIGLVV 491

Query: 486 SNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWT 545
           SNSIWFLSTL LWLGVPQAKKP VWGVPLVPW+PSLSIAINLFLLGSIDKASFERFG+WT
Sbjct: 492 SNSIWFLSTLALWLGVPQAKKPHVWGVPLVPWLPSLSIAINLFLLGSIDKASFERFGIWT 551

Query: 546 GILLIYYFLIGLHASYDSVMESKTEAVRVNN 570
           GILL+YY LIGLHASYDSVMESKT A  VN+
Sbjct: 552 GILLVYYILIGLHASYDSVMESKTIAGEVNS 582

BLAST of Cp4.1LG09g07150 vs. TAIR 10
Match: AT4G21120.1 (amino acid transporter 1 )

HSP 1 Score: 810.1 bits (2091), Expect = 1.2e-234
Identity = 403/567 (71.08%), Postives = 471/567 (83.07%), Query Frame = 0

Query: 5   EEGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARS 64
           ++G+RRR C C+K+DFLPEESF+S  NY KALK TP+R +DR+  RS +  ++ EMKARS
Sbjct: 7   DDGLRRRGCSCTKDDFLPEESFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINEMKARS 66

Query: 65  QHEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCY 124
            HEMKK+LTWWDLMWFGIGAVIG+GIFVLTGLE + H+GPA+VLSYVVSGVSAMLSVFCY
Sbjct: 67  GHEMKKTLTWWDLMWFGIGAVIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCY 126

Query: 125 TEFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHP 184
           TEFAVEIP AGGSFAYLRVELGDFMAFI AGNI+LEYV+GGAAVARSWTSYFATL NH P
Sbjct: 127 TEFAVEIPVAGGSFAYLRVELGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATLLNHKP 186

Query: 185 NDFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIA 244
            DFRI V    +DY+ LDPIA+ V  +IC LAV  TKGSSRFNYIASI H+V+ILF+IIA
Sbjct: 187 EDFRIIVHKLGEDYSHLDPIAVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIA 246

Query: 245 GLTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSM 304
           G TKA+ KN++ F PYG RG+F ++AVLFFAY+GFDAVSTMAEETKNP +DIPIGLVGSM
Sbjct: 247 GFTKADVKNYSDFTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSM 306

Query: 305 VITT------XVTLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVS 364
           V+TT       VTLCLMQPYQQID DAPFSVAF AVGW WAKYIVA GA+KGMTTVLLV 
Sbjct: 307 VVTTVCYCLMAVTLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVG 366

Query: 365 AVGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSI 424
           A+GQARY+THIAR HM+ PW A+V+ +T  P+NAT  MLAATA+IAFFT L IL++LLS+
Sbjct: 367 AIGQARYMTHIARAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSV 426

Query: 425 STLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWS-SSDGWIGL 484
           STLFIFM VAV L+VRRYYV+GET+  DRNK ++ + LIL SS ATAVYW+   +GWIG 
Sbjct: 427 STLFIFMFVAVALLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEEGWIGY 486

Query: 485 VVSNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGL 544
            ++  IWFLST+ +   VPQA+ P++WGVPLVPW+PS SIAIN+FLLGSID  SF RF +
Sbjct: 487 CITVPIWFLSTVAMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAI 546

Query: 545 WTGILLIYYFLIGLHASYDSVMESKTE 565
           WTGILLIYY L GLHA+YD+   +  E
Sbjct: 547 WTGILLIYYVLFGLHATYDTAKATLKE 573

BLAST of Cp4.1LG09g07150 vs. TAIR 10
Match: AT2G34960.1 (cationic amino acid transporter 5 )

HSP 1 Score: 624.0 bits (1608), Expect = 1.2e-178
Identity = 322/559 (57.60%), Postives = 412/559 (73.70%), Query Frame = 0

Query: 6   EGIRRRSCGCSKEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQ 65
           EG  R     SK DF PEESF+S+ +Y  AL  T +R  +RL +RS +  +  E+K +S+
Sbjct: 2   EGEERGYWRWSKRDFFPEESFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSE 61

Query: 66  HEMKKSLTWWDLMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYT 125
           HEMK+ LTWWDL+WFG G+VIGAGIFVLTG E  + AGPAIVLSYVVSG+SAMLSVFCYT
Sbjct: 62  HEMKRCLTWWDLVWFGFGSVIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYT 121

Query: 126 EFAVEIPAAGGSFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPN 185
           EFAVEIP AGGSFAYLR+ELGDF AFITAGNILLE ++G AAVAR+WTSYFATL N  PN
Sbjct: 122 EFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPN 181

Query: 186 DFRIHVSSFPDDYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAG 245
             RI  +     +N LDPIA+VVI    T+A  ST+ +S  N+IAS  + ++I F+IIAG
Sbjct: 182 ALRIK-TDLSSGFNLLDPIAVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAG 241

Query: 246 LTKANPKNFTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMV 305
              A+  N TPF P+GP G+F A+AV++FAY GFD+++TMAEETKNP++DIPIGL+GSM 
Sbjct: 242 FIHADTSNLTPFLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMS 301

Query: 306 ITTXV------TLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSA 365
           I T +      +L +MQ Y  ID +A +SVAF++VG  W KY+VA+GA+KGMTTVLLV A
Sbjct: 302 IITVIYCLMALSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGA 361

Query: 366 VGQARYLTHIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSIS 425
           +GQARY+THIARTHMI P FA VH +T  P+NA   +   +A+IAFF+ L +L++LLSIS
Sbjct: 362 LGQARYVTHIARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSIS 421

Query: 426 TLFIFMLVAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYW--SSSDGWIGL 485
           TLFIF ++ + L+VRRYYV  +T      KLI C++ ++ SS+ T+ YW       WIG 
Sbjct: 422 TLFIFTMMPIALLVRRYYVRQDTPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGY 481

Query: 486 VVSNSIWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGL 545
            V+   WFL TL +   VPQ + P+VWGVPLVPW+P LSIA N+FL+GS+   +F RFG+
Sbjct: 482 TVTVPFWFLGTLGIVFFVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGV 541

Query: 546 WTGILLIYYFLIGLHASYD 557
            T  +L+YYFL+GLHA++D
Sbjct: 542 CTLAMLLYYFLLGLHATFD 559

BLAST of Cp4.1LG09g07150 vs. TAIR 10
Match: AT1G17120.1 (cationic amino acid transporter 8 )

HSP 1 Score: 552.0 bits (1421), Expect = 5.7e-157
Identity = 285/556 (51.26%), Postives = 388/556 (69.78%), Query Frame = 0

Query: 17  KEDFLPEESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQHEMKKSLTWWD 76
           K+DF PE SF+S+  Y  AL AT  RL DRL +RS +  +L   +  S++ M++ LTWWD
Sbjct: 30  KQDFFPEFSFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWD 89

Query: 77  LMWFGIGAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYTEFAVEIPAAGG 136
           L+W   G+V+G+G+FV+TG E +  AGPA+VLSY +SGVSA+LSV CY EF VEIP AGG
Sbjct: 90  LLWLSFGSVVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGG 149

Query: 137 SFAYLRVELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPNDFRIHVSSFPD 196
           SF+YLRVELGDF+AFI AGNILLE ++G A + RSW+SY A+L  +  + FRI V SF  
Sbjct: 150 SFSYLRVELGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYFRIKVDSFAK 209

Query: 197 DYNQLDPIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAGLTKANPKNFTP 256
            ++ LDP+A+ V++V   +A+  TK +S  N I S+  V II+FI++ G T +   N  P
Sbjct: 210 GFDLLDPVAVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP 269

Query: 257 FAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMVITTXV------ 316
           F PYG +G+  ++AV++++Y GFD V+ MAEET+ P++DIPIGLVGSM + T V      
Sbjct: 270 FFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMAL 329

Query: 317 TLCLMQPYQQIDEDAPFSVAFEAVGWGWAKYIVAVGAIKGMTTVLLVSAVGQARYLTHIA 376
            L +M  Y +ID +A +SVAF  +G  WAKY+V + A+KGMTT LLV ++GQARY T IA
Sbjct: 330 ALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIA 389

Query: 377 RTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSISTLFIFMLVAVG 436
           R+HMI PWFA VH +T  P+ AT  +   ++II+FFTSL +LS++ S +TLFIFMLVAV 
Sbjct: 390 RSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAVA 449

Query: 437 LIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSS-DGWIGLVVSNSIWFLSTL 496
           L+VRRYYV   T  +   K +  + LI+ SSI  +  W+S   GWI   V+  IWF+ TL
Sbjct: 450 LLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVTGVIWFIGTL 509

Query: 497 VLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWTGILLIYYFLI 556
            L L +P+ + P+VWGVPLVPW+PS SIA+NLFL+GS+   +F RF + T ++L+YY  +
Sbjct: 510 GLAL-LPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLYYLFV 569

Query: 557 GLHASYDSVMESKTEA 566
           GLHA+YD   +   EA
Sbjct: 570 GLHATYDVAHQPLEEA 584

BLAST of Cp4.1LG09g07150 vs. TAIR 10
Match: AT5G04770.1 (cationic amino acid transporter 6 )

HSP 1 Score: 435.6 bits (1119), Expect = 6.0e-122
Identity = 232/552 (42.03%), Postives = 350/552 (63.41%), Query Frame = 0

Query: 25  SFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQHEMKKSLTWWDLMWFGIGA 84
           SF S R Y  +L ATP+RL  R  + S    ++  ++A S  +M+++L W+DL+  GIG 
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 85  VIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYTEFAVEIPAAGGSFAYLRVE 144
           ++GAG+FV TG  ++  AGP+IV+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R+ 
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 145 LGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPNDFRIHVSSFPDDYNQLDPI 204
            G+F AF T  N++++YV+  AAV+RS+T+Y  T      + +R  VS  P  +N++DP+
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDPV 193

Query: 205 AIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAGLTK---------ANPKNFT 264
           A++V++VI  +   ST+ SS+ N I + FH+  I F+I+ G  K         ANP++ +
Sbjct: 194 AVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPS 253

Query: 265 PFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMVITT------X 324
            F P+G  G+F  +A+++ +Y+G+DAVSTMAEE +NP KDIP+G+ GS+ I T       
Sbjct: 254 GFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMA 313

Query: 325 VTLCLMQPYQQIDEDAPFSVAFE-AVGWGWAKYIVAVGAIKGMTTVLLVSAVGQARYLTH 384
           V++ ++ PY  ID +APFS AF  + GW W   +V +GA  G+ T LLV+ +GQARY+  
Sbjct: 314 VSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCV 373

Query: 385 IARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSISTLFIFMLVA 444
           I R+ ++  WFAK+H +TS PVNA+  +   TA +A FT L +L NL+SI TLF+F +VA
Sbjct: 374 IGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVA 433

Query: 445 VGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYWSSSDG---WIGLVVSNSIWF 504
             LI RRY   G T P     L    +  + S + T ++    +G      L  S  +  
Sbjct: 434 NALIFRRYVPVGPTKPWP--TLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVAI 493

Query: 505 LSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWTGILLIY 558
              L     VPQA+KP +WGVP +PW P +SI +N+FLLGS+D  S+ RFG ++G++++ 
Sbjct: 494 AIVLSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVLV 553

BLAST of Cp4.1LG09g07150 vs. TAIR 10
Match: AT3G10600.1 (cationic amino acid transporter 7 )

HSP 1 Score: 433.7 bits (1114), Expect = 2.3e-121
Identity = 226/555 (40.72%), Postives = 353/555 (63.60%), Query Frame = 0

Query: 23  EESFKSWRNYSKALKATPTRLLDRLTARSGEHTKLVEMKARSQHEMKKSLTWWDLMWFGI 82
           + SF S R Y  +L  TP+R   R  + S  + ++  ++A S  +M+++L W+DL+  GI
Sbjct: 11  DTSFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGI 70

Query: 83  GAVIGAGIFVLTGLETKKHAGPAIVLSYVVSGVSAMLSVFCYTEFAVEIPAAGGSFAYLR 142
           G +IGAG+FV TG  ++ +AGP+IV+SY ++G+ A+LS FCYTEFAV +P AGG+F+Y+R
Sbjct: 71  GGMIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIR 130

Query: 143 VELGDFMAFITAGNILLEYVIGGAAVARSWTSYFATLCNHHPNDFRIHVSSFPDDYNQLD 202
           +  G+F AFIT  N++++YV+  AAV+R +T+Y  +      +++R  VS  P+ +N++D
Sbjct: 131 ITFGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEID 190

Query: 203 PIAIVVIIVICTLAVASTKGSSRFNYIASIFHVVIILFIIIAGLTKA---------NPKN 262
           PIA++V++ +  +   ST+ SS+ N + +  H+  I+F+I+ G +K          NP+N
Sbjct: 191 PIAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPEN 250

Query: 263 FTPFAPYGPRGIFVASAVLFFAYVGFDAVSTMAEETKNPAKDIPIGLVGSMVIT------ 322
            + F P+G  G+F  +A+++ +Y+G+DAVSTMAEE K+P KDIP+G+ GS+ I       
Sbjct: 251 PSGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCL 310

Query: 323 TXVTLCLMQPYQQIDEDAPFSVAF-EAVGWGWAKYIVAVGAIKGMTTVLLVSAVGQARYL 382
             +++ ++ PY  ID +AP+S AF ++ GW W   +V +GA  G+ T L+V+ +GQARY+
Sbjct: 311 MAISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYM 370

Query: 383 THIARTHMISPWFAKVHERTSIPVNATATMLAATAIIAFFTSLGILSNLLSISTLFIFML 442
             I R+ ++  WFAKVH +TS PVNA+A +   TA++A FT L +L NL+SI TLF+F +
Sbjct: 371 CVIGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYM 430

Query: 443 VAVGLIVRRYYVSGETTPSDRNKLIICMVLILGSSIATAVYW----SSSDGWIGLVVSNS 502
           VA  +I RRY   G T P       +C+  I  +SI   + W    S    W  L  S  
Sbjct: 431 VANAVIFRRYVTVGYTEPWP-TLSFLCLFSI--TSILFTLVWQLAPSGPPKWFILGASTV 490

Query: 503 IWFLSTLVLWLGVPQAKKPRVWGVPLVPWVPSLSIAINLFLLGSIDKASFERFGLWTGIL 558
                  +    VPQA+ P  WGVPL+PW P +SI +N+FLLGS+D  S+ RFG ++G++
Sbjct: 491 TAIAIVQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLV 550