Cp4.1LG01g17770 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g17770
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCp4.1LG01: 13994113 .. 13998593 (+)
RNA-Seq ExpressionCp4.1LG01g17770
SyntenyCp4.1LG01g17770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTGTTCGGTGGATTAGTTGGAAAAGGAATGCAGTTGACGACTGTGCCTAGATAAGTCGATCGTCAAAGATCGGCTCTCTAATACCGCCGCTGGGAGAACCGAAGCCTCATTCCATTGAAATAATTCCTCTGATATAGACTGTTCTTACTTCAGTCTTAAGAACGGGAAAGGGGAAAGGAGTATATACACACATCTGGGTTTCATCTTCCTCGTTCAAGCGTCAATGTCACTCCTCAACCAACTCTTCAACAGAGGCGTCTTTGCGGGCAAATGGTCAGCTTTCGATTTTTCTAATTCTAAACCTCCTCACTATTTCATTAACGATGCTTCAATTTGGTTTCGGATCCTCTGCATTACTTAGAATTCGTCTGGGATTGTTATCTTCCCGTGGATTTTTCTGTTCGTAGGAGAATCTTTTTGTTTCGTTAATTTACCAAATCCAACACCCACAAACGAAATTTCTGTTGAGATTTTGCCCAAATTTTCGTTCGGCTAAACTTCGACAAAATAATATATCGACCAGTTTTCCAGTTTTTGTATAATTTCGGCAGTTGAATTTTGATTCGTACTCTTCTACCTTTATCATCTCTCGATTAGACTGGGGGTTTTCCGCACAATTTCATGTCAAAATCTATAATCAAACAAGTACTTGTAAACAGATAGCCATCCTTGCATTTTTCTGGGAATGAAAGGTCTAATATATATTGGGTGTTTTGTAGCAAAACTTGCTTGAGCTTGGCAATCTCGCGAATCAAGCTGCTGCAGAATAAGAGGGATCTACAGCTGAAGCATATGCGCAAGGAGATCGCACAATTCTTGCAGACGGGGCAGGAACCAATAGCTCGGATTCGGGTACCCTTTAGAATTTTGAGATCTGTAATGTCTGTTAGTTCTAATGTTTCTTTTACTATCCCGTAAAATAACATGTGTAGTCATCTGTTTGGGCAGGTGGAGCATGTAATCCGGGAACAAAACATATGGGCAGCATATGAAATTCTAGAGTTGTTTTGTGAATTTGTTCTTGCTCGTGTCCCCATCATCGAAAGCCAGAAGTAATCTATAGCTCAAAAGTTGTTTTGTGATTTTTTTTTGCTTCTTGGAGTTTCTATTTATATGTTGGTTCACATAAATTGGGACTCCCTTGGTCCAATGGATGTCTGATGATTCTTAGCATTTGGATCTTTAAATAAGTCATGGAAGCTCTTGTTTAACTGGTTCATCTCCATTAATTTTTTGTTTGTCCCTATTAAAAGTTAATTAACTATGAAACTAACTTCTAACTGATATGTGGTTCTGAAGCTTACTTGTGATCCCCAGTCAAAACCTTCTCTGTTTGTGTTGGACTTTAATATCGATTGATGCGTCCTTTACATTTTCCGGTGCATCTTTCGGCTGGGTTATGTTGTAGAATTATAAATTATGTGGTGCTTTCTTTGGTGCTGATGTCTAATTTAATAGTCTTGAGAAAATCTGTTAGCTGCAAAGTTATTTCAGTTACACTGTTCTAAGCTACAAAAGAAGAAAGTGGTACCTGTTCATTCAATCAAAGGAACTAGTGTCGTGTCTATCTACCAAAATTTATTCACTCAATTTTCTTGTCAGTACTAAGTTGTCATTGACATAATTTTCAACGGTAGTATGTATTTGTGAGTTCTCTTGGATAATTATGATAGGAACAGCAGTAGGAATATTATTAGGGTATTAAGGGTTTATTAGTAATTAGTTAGGAAGATTGTTAGGGTATTTGATTCTAAATAGAGGGAGTAGGAAAAGAGGAAGTTAGACAATTGTTTAGTGGTTCGGGCTTCGGTGGCATCTTAAGAAAGTGGAGGTTCAAAGTACCTCTAATACTTGGGAGTTATCTTGCTGTTCTGTTATCTTTCATATTTCAATGTACTTGGGTTCTATCAAATTGTTGTTAAGATTAGTGTTCCAATGCTATGATGTGAAGATGAGTTCTATTAATTTGCTTGGTTTGATTTGATGCATGTGTTTTTCATTATTTGCTTAGAAACCTGTAGCCTTGGCATAATTAAAAAAATGGTGAGGTTAATCTGTTGAGTCCTTTTCGTGCAGACCATACTGGGGGCCTTTTATGTGAATGTTCTTACTTTGTGCATTTTGTTTGTCCGTAACTAACTATTAAATTTACAATAACAGAGAATGTCCAGTCGAACTGCGGGAGGCTATTGCGAGCATAATATTTGCTGCTCCTAGATGTTCTGACCTTCCAGATCTACTGCAGTTGAAGAATTTGTTTGCTGTCAAATATGGGAAAGAATTTGTCTCTGCTATTTCTGAGCTTCGTCCTGACTCCGGTGTTAATCGATCAGTTGAGTGTGTCTCAAGTTTATCGTGAGCAGTAGTAATGTTTGTTTTTTTAGAATAAAAGCGAAATGGTTCATTCAATTCTTTGTTTTCATTTCAGATTATTGAAAAGCTTGCAGTGAGTGCGCCTCCTGGAGAACTAAAGCTCAAGGTCATGAAGGAGATTGCAAAAGAACATGGACTCGACTGGGATTCTTCCTCCACTGCTTCTGAATTTAGTAAAACCCACGAAGATCTGCTGGTATGATTCCCAATTTGATTTTGGTGTTGATTGATCTGAAACACACATTCATACTACTATAGCAAAGTTATAAATGACATATATTCTTGCTTCTGGAAACAATTATCCCATTCTACTGACTGCATATTTCTCCATCCTTTCCCTCATATATTGGCATGATATTTCTAACCGCTGTTCCTGAAAAGCCTGAAAGGAACTGAGATTTGAACTACAGAGCTGAATTGGAAAATGCTTGTATTGGAATGAAAATGGTCTAGATTCTATGATTTTTGAGAGATCAAGAGCTCTCCTTTCTCCAGGGGATCCTCTATAATCTTTGAACAAAACTCTGTGGCCCTCCCCCGTTTTCCTATCCGTAACTGTCGAACCCTAACAAATTGCTGACACACGCTCAAAAACAGTAAGATCAACAAGCTAACTAATTCGTTCACAGTACTAGAGATTACAACTTCTGTTGAAAATTTTTGTTCTGTCTGCTGAAAAGAAGAATATTATCATATGAGACCCTCAACTGTGAATACGTACTTTACGAGAGGTAGTAAGGAAAAGGACCACCAGACAATTAGTTGGTGGAACACTCGTACCAGTGGCATGGAGGTGAGGACTAGCCATTGGAGGGAAGTATTCTGTTTTGGGAGGATGACTTTTCTTTTGAATAGAACTGTGTTTTATGGGCTGAGAGGTTACCAGCCATCTTGAAATTGGCTGGGAGTTTGTTTGGGTTCTTTGTGTTATTATGAACTTTGCATCTGTTTTTTCAATTTAATGAAGTTGTAAGGAAGGCATCTTACACATTATTACACCTACTTTTGACTAACTACTTTGTCAAGTTCAACCTTAAATATTAAATGTTATCAATTTGAACCCTGAACTTAAATAGATTGCACAATTAGTATCCTCAAGTTCAAAGTTACATTTTTAGAATATTTGTGGCTGGCTATGAGCATTTTGTGACTAAACTAATCTAGTTTTACGAGAATTAAATCTTCATGTTGGGTACTTTTAGCTTGATCTGCGCAACACACACACACAAAATGAAGTTTATTGTCATGGTAACTTATTCTGTTCTTGATGGTCTATTCTGTGGCCTTTGTCATGGGATATGCAGGGTGGACCAAAGGTAGTACATGGAGCAGCAGCGTCTCAAGCTCCCTCTCAATATAGCTCGTCCAATCCTTCCACTCCTTATGGAGCGTTGCATCCCGTTGTGTCTCCTGTAGAAAAGCAAGAACCTGAATGCCACCATACGTTCACCCGAATTAGCAATAGCGCAAGTGATATCAAACCGGGACCTAAGAATTTTGATGTCAAACCAGAAGTCGCTTCTAAATTAGATACAAGACCACCTCCATGGGATGTATTAGAGAAAGCCAGAGCTGCCATTGCTTCAGCCGAACGTGCCACTGCAGCAGCCCGAGTTGCTGCCGAGCTAGCCAATGTCAACCTTGGAGCAATGAAGCTTGAAGGAGGAGGACAAGCTTCTGAGTGCTGAAGAGAAAAAGAAAGGTAATTGATACATTGTTTGATACTGGACTGGTGCATAAATTGTGTTTGAAGTTAATCGGGTGTCCAAAGATTGAAGAGTGGAGAGTGGGTCAATCCATAAGTGAAAAAAAACCCGTGTAATTAATAGAGTTGCAAGGAAAATTTAATTCCAAGTATTTGTAAAATACTAAAATTCTTTGGTGATTATGGTGAAGTTCTAATGGTGCAGCAGTTTGATATAAATGCAACATTAGAGGAAAATAAGTTTGCTTTAATTTAAGATAAGTTGCAGACTGAGGAAGGGTAAAGTATTCGCAATGTTCTTCCTGAAACTATGAGCATCCATTGTAGAAATACTCACAAGTTTGCTTGAGAAATTGTGGATTGCCATCAGTTTCTGGTTAAAATGAAGCACCGTTGCCAC

mRNA sequence

TTTTTGTTCGGTGGATTAGTTGGAAAAGGAATGCAGTTGACGACTGTGCCTAGATAAGTCGATCGTCAAAGATCGGCTCTCTAATACCGCCGCTGGGAGAACCGAAGCCTCATTCCATTGAAATAATTCCTCTGATATAGACTGTTCTTACTTCAGTCTTAAGAACGGGAAAGGGGAAAGGAGTATATACACACATCTGGGTTTCATCTTCCTCGTTCAAGCGTCAATGTCACTCCTCAACCAACTCTTCAACAGAGGCGTCTTTGCGGGCAAATGCAAAACTTGCTTGAGCTTGGCAATCTCGCGAATCAAGCTGCTGCAGAATAAGAGGGATCTACAGCTGAAGCATATGCGCAAGGAGATCGCACAATTCTTGCAGACGGGGCAGGAACCAATAGCTCGGATTCGGGTGGAGCATGTAATCCGGGAACAAAACATATGGGCAGCATATGAAATTCTAGAGTTGTTTTGTGAATTTGTTCTTGCTCGTGTCCCCATCATCGAAAGCCAGAAAGAATGTCCAGTCGAACTGCGGGAGGCTATTGCGAGCATAATATTTGCTGCTCCTAGATGTTCTGACCTTCCAGATCTACTGCAGTTGAAGAATTTGTTTGCTGTCAAATATGGGAAAGAATTTGTCTCTGCTATTTCTGAGCTTCGTCCTGACTCCGGTGTTAATCGATCAATTATTGAAAAGCTTGCAGTGAGTGCGCCTCCTGGAGAACTAAAGCTCAAGGTCATGAAGGAGATTGCAAAAGAACATGGACTCGACTGGGATTCTTCCTCCACTGCTTCTGAATTTAGTAAAACCCACGAAGATCTGCTGGGTGGACCAAAGGTAGTACATGGAGCAGCAGCGTCTCAAGCTCCCTCTCAATATAGCTCGTCCAATCCTTCCACTCCTTATGGAGCGTTGCATCCCGTTGTGTCTCCTGTAGAAAAGCAAGAACCTGAATGCCACCATACGTTCACCCGAATTAGCAATAGCGCAAGTGATATCAAACCGGGACCTAAGAATTTTGATGTCAAACCAGAAGTCGCTTCTAAATTAGATACAAGACCACCTCCATGGGATGTATTAGAGAAAGCCAGAGCTGCCATTGCTTCAGCCGAACGTGCCACTGCAGCAGCCCGAGTTGCTGCCGAGCTAGCCAATGTCAACCTTGGAGCAATGAAGCTTGAAGGAGGAGGACAAGCTTCTGAGTGCTGAAGAGAAAAAGAAAGGTAATTGATACATTGTTTGATACTGGACTGGTGCATAAATTGTGTTTGAAGTTAATCGGGTGTCCAAAGATTGAAGAGTGGAGAGTGGGTCAATCCATAAGTGAAAAAAAACCCGTGTAATTAATAGAGTTGCAAGGAAAATTTAATTCCAAGTATTTGTAAAATACTAAAATTCTTTGGTGATTATGGTGAAGTTCTAATGGTGCAGCAGTTTGATATAAATGCAACATTAGAGGAAAATAAGTTTGCTTTAATTTAAGATAAGTTGCAGACTGAGGAAGGGTAAAGTATTCGCAATGTTCTTCCTGAAACTATGAGCATCCATTGTAGAAATACTCACAAGTTTGCTTGAGAAATTGTGGATTGCCATCAGTTTCTGGTTAAAATGAAGCACCGTTGCCAC

Coding sequence (CDS)

ATGTCACTCCTCAACCAACTCTTCAACAGAGGCGTCTTTGCGGGCAAATGCAAAACTTGCTTGAGCTTGGCAATCTCGCGAATCAAGCTGCTGCAGAATAAGAGGGATCTACAGCTGAAGCATATGCGCAAGGAGATCGCACAATTCTTGCAGACGGGGCAGGAACCAATAGCTCGGATTCGGGTGGAGCATGTAATCCGGGAACAAAACATATGGGCAGCATATGAAATTCTAGAGTTGTTTTGTGAATTTGTTCTTGCTCGTGTCCCCATCATCGAAAGCCAGAAAGAATGTCCAGTCGAACTGCGGGAGGCTATTGCGAGCATAATATTTGCTGCTCCTAGATGTTCTGACCTTCCAGATCTACTGCAGTTGAAGAATTTGTTTGCTGTCAAATATGGGAAAGAATTTGTCTCTGCTATTTCTGAGCTTCGTCCTGACTCCGGTGTTAATCGATCAATTATTGAAAAGCTTGCAGTGAGTGCGCCTCCTGGAGAACTAAAGCTCAAGGTCATGAAGGAGATTGCAAAAGAACATGGACTCGACTGGGATTCTTCCTCCACTGCTTCTGAATTTAGTAAAACCCACGAAGATCTGCTGGGTGGACCAAAGGTAGTACATGGAGCAGCAGCGTCTCAAGCTCCCTCTCAATATAGCTCGTCCAATCCTTCCACTCCTTATGGAGCGTTGCATCCCGTTGTGTCTCCTGTAGAAAAGCAAGAACCTGAATGCCACCATACGTTCACCCGAATTAGCAATAGCGCAAGTGATATCAAACCGGGACCTAAGAATTTTGATGTCAAACCAGAAGTCGCTTCTAAATTAGATACAAGACCACCTCCATGGGATGTATTAGAGAAAGCCAGAGCTGCCATTGCTTCAGCCGAACGTGCCACTGCAGCAGCCCGAGTTGCTGCCGAGCTAGCCAATGTCAACCTTGGAGCAATGAAGCTTGAAGGAGGAGGACAAGCTTCTGAGTGCTGA

Protein sequence

MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQEPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC
Homology
BLAST of Cp4.1LG01g17770 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 68/182 (37.36%), Postives = 109/182 (59.89%), Query Frame = 0

Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G+ A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

BLAST of Cp4.1LG01g17770 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-24
Identity = 68/182 (37.36%), Postives = 108/182 (59.34%), Query Frame = 0

Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

BLAST of Cp4.1LG01g17770 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-24
Identity = 68/182 (37.36%), Postives = 108/182 (59.34%), Query Frame = 0

Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

BLAST of Cp4.1LG01g17770 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-24
Identity = 68/182 (37.36%), Postives = 108/182 (59.34%), Query Frame = 0

Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

BLAST of Cp4.1LG01g17770 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.7e-23
Identity = 67/182 (36.81%), Postives = 107/182 (58.79%), Query Frame = 0

Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY K +   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKGY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

BLAST of Cp4.1LG01g17770 vs. NCBI nr
Match: XP_023538831.1 (IST1 homolog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634 bits (1634), Expect = 9.66e-229
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240

Query: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300
           EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA
Sbjct: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 327

BLAST of Cp4.1LG01g17770 vs. NCBI nr
Match: KAG6601778.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615 bits (1586), Expect = 2.00e-221
Identity = 320/327 (97.86%), Postives = 321/327 (98.17%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYG  HPVVS VEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGTAHPVVSVVEKQ 240

Query: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300
           EPECHHTFTRISNSASDIKPGPKN DVKPE ASKLDTRPPP DVLEKARAAIASAERATA
Sbjct: 241 EPECHHTFTRISNSASDIKPGPKNSDVKPEFASKLDTRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMK+EGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKVEGGGQASEC 327

BLAST of Cp4.1LG01g17770 vs. NCBI nr
Match: XP_022921725.1 (IST1 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 612 bits (1577), Expect = 4.71e-220
Identity = 319/327 (97.55%), Postives = 319/327 (97.55%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQ PSQYSSSNPSTPYGA HPVVS VEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQVPSQYSSSNPSTPYGAAHPVVSVVEKQ 240

Query: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300
           EPECHHT TRISNS SDIKPGPKNFDVK E ASKLDTRPPP DVLEKARAAIASAERATA
Sbjct: 241 EPECHHTLTRISNSVSDIKPGPKNFDVKLEFASKLDTRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 327

BLAST of Cp4.1LG01g17770 vs. NCBI nr
Match: XP_022971809.1 (IST1 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 606 bits (1562), Expect = 8.76e-218
Identity = 319/327 (97.55%), Postives = 320/327 (97.86%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+MRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYG   PVVS VEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGES-PVVSVVEKQ 240

Query: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300
           EPECHHTFTR SNSASDIKPGPKNFDVKPEVASKLD RPPP DVLEKARAAIASAERATA
Sbjct: 241 EPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 326

BLAST of Cp4.1LG01g17770 vs. NCBI nr
Match: KAG7032495.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 606 bits (1562), Expect = 1.47e-217
Identity = 320/340 (94.12%), Postives = 321/340 (94.41%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRS-------------IIEKLAVSAPPGEL 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRS             IIEKLAVSAPPGEL
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSVECVSSLSFSSSNIIEKLAVSAPPGEL 180

Query: 181 KLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPY 240
           KLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPY
Sbjct: 181 KLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPY 240

Query: 241 GALHPVVSPVEKQEPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEK 300
           G  HPVVS VEKQEPECHHTFTRISNSASDIKPGPKN DVKPE ASKLDTRPPP DVLEK
Sbjct: 241 GTAHPVVSVVEKQEPECHHTFTRISNSASDIKPGPKNSDVKPEFASKLDTRPPPSDVLEK 300

Query: 301 ARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 327
           ARAAIASAERATAAARVAAELANVNLGAMK+EGGGQASEC
Sbjct: 301 ARAAIASAERATAAARVAAELANVNLGAMKVEGGGQASEC 340

BLAST of Cp4.1LG01g17770 vs. ExPASy TrEMBL
Match: A0A6J1E6L9 (IST1 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 2.28e-220
Identity = 319/327 (97.55%), Postives = 319/327 (97.55%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQ PSQYSSSNPSTPYGA HPVVS VEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQVPSQYSSSNPSTPYGAAHPVVSVVEKQ 240

Query: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300
           EPECHHT TRISNS SDIKPGPKNFDVK E ASKLDTRPPP DVLEKARAAIASAERATA
Sbjct: 241 EPECHHTLTRISNSVSDIKPGPKNFDVKLEFASKLDTRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 327

BLAST of Cp4.1LG01g17770 vs. ExPASy TrEMBL
Match: A0A6J1I9L3 (IST1 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1)

HSP 1 Score: 606 bits (1562), Expect = 4.24e-218
Identity = 319/327 (97.55%), Postives = 320/327 (97.86%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+MRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYG   PVVS VEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGES-PVVSVVEKQ 240

Query: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300
           EPECHHTFTR SNSASDIKPGPKNFDVKPEVASKLD RPPP DVLEKARAAIASAERATA
Sbjct: 241 EPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 326

BLAST of Cp4.1LG01g17770 vs. ExPASy TrEMBL
Match: A0A6J1DR13 (IST1 homolog OS=Momordica charantia OX=3673 GN=LOC111022352 PE=3 SV=1)

HSP 1 Score: 539 bits (1389), Expect = 9.32e-192
Identity = 284/328 (86.59%), Postives = 302/328 (92.07%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHM KEIAQFLQ GQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMHKEIAQFLQAGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQ+ECPVELREAI+SIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQRECPVELREAISSIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAIS+LRPDSGVNRSIIEKL+VSAPPGELKLKVMKEIA+EHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISDLRPDSGVNRSIIEKLSVSAPPGELKLKVMKEIAQEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           L+WDSS+TASEFSKTHEDLLGGPKVVHGAAASQ P Q+ SSN S   G   PVVSP  KQ
Sbjct: 181 LNWDSSNTASEFSKTHEDLLGGPKVVHGAAASQTPLQHGSSNSS---GTARPVVSPTGKQ 240

Query: 241 EPECHHTFTRISNSA-SDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERAT 300
           EP+ HHTF+ I+N+  ++IKP  KNF+ KPEV +KLDT PPP D+LEKARAAIASAERAT
Sbjct: 241 EPDRHHTFSPINNTTINEIKPASKNFNFKPEVDAKLDTIPPPSDILEKARAAIASAERAT 300

Query: 301 AAARVAAELANVNLGAMKLEGGGQASEC 327
           AAARVAAEL NVNLGAMKLEGG QAS+C
Sbjct: 301 AAARVAAELTNVNLGAMKLEGGAQASDC 325

BLAST of Cp4.1LG01g17770 vs. ExPASy TrEMBL
Match: A0A6J1E1A6 (IST1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1)

HSP 1 Score: 533 bits (1374), Expect = 4.29e-190
Identity = 278/286 (97.20%), Postives = 278/286 (97.20%), Query Frame = 0

Query: 42  MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 101
           MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE
Sbjct: 1   MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 60

Query: 102 LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 161
           LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS
Sbjct: 61  LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 120

Query: 162 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS 221
           APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQ PSQYSSS
Sbjct: 121 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQVPSQYSSS 180

Query: 222 NPSTPYGALHPVVSPVEKQEPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPP 281
           NPSTPYGA HPVVS VEKQEPECHHT TRISNS SDIKPGPKNFDVK E ASKLDTRPPP
Sbjct: 181 NPSTPYGAAHPVVSVVEKQEPECHHTLTRISNSVSDIKPGPKNFDVKLEFASKLDTRPPP 240

Query: 282 WDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 327
            DVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 241 SDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 286

BLAST of Cp4.1LG01g17770 vs. ExPASy TrEMBL
Match: A0A6J1I6R9 (IST1 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 6.84e-189
Identity = 279/286 (97.55%), Postives = 279/286 (97.55%), Query Frame = 0

Query: 42  MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 101
           MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE
Sbjct: 1   MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 60

Query: 102 LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 161
           LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS
Sbjct: 61  LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 120

Query: 162 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS 221
           APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS
Sbjct: 121 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS 180

Query: 222 NPSTPYGALHPVVSPVEKQEPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPP 281
           NPSTPYG   PVVS VEKQEPECHHTFTR SNSASDIKPGPKNFDVKPEVASKLD RPPP
Sbjct: 181 NPSTPYGES-PVVSVVEKQEPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPP 240

Query: 282 WDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 327
            DVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 241 SDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 285

BLAST of Cp4.1LG01g17770 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 359.0 bits (920), Expect = 4.1e-99
Identity = 198/317 (62.46%), Postives = 235/317 (74.13%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRG+F  KCKT L+LAI+R+KLLQNKRD+QLKHM+KEIA FLQ GQEPIARI
Sbjct: 1   MSLLNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIRE N+WAAYEILELFCEF+LARVPI+ES+KECP ELREAIASIIFAAPRCS++P
Sbjct: 61  RVEHVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQ+KNLF  KYGKEF+   SELRPDSGVNR+IIEKL+ ++P G  +LK++KEIA+E+ 
Sbjct: 121 DLLQIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYS 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240
           L+WDSS+T +EF K+HEDLLGG K +H           S S PS        V   VE  
Sbjct: 181 LNWDSSATEAEFMKSHEDLLGGAKQIH------RQDGISESRPSQQGYGQSSVSREVESL 240

Query: 241 EPECHHTFTRI--SNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERA 300
             E    F ++   N  S   P  K   +     +  DTR    DV+E ARAA+ASA+RA
Sbjct: 241 PAEATQRFQKLQAQNPVSKSMPSSK---LTSAFQAPPDTRRNQTDVMEIARAALASADRA 300

Query: 301 TAAARVAAELANVNLGA 316
           TAAAR AA+L NV+ GA
Sbjct: 301 TAAARAAAQLVNVSYGA 308

BLAST of Cp4.1LG01g17770 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.3e-68
Identity = 164/404 (40.59%), Postives = 220/404 (54.46%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MS+L+  FN+G  A KCKT L L I RIKL++N+R+ Q+K MR+EIA+ L+TGQE  ARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEH+IRE+ + AA EILELFCE +  R+PIIE+Q+ECP++L+EAI+S+ FAAPRCSDL 
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           +L Q++ LF  KYGKEFV+A SEL+PDSGVNR ++E L+V AP  E KLK++KEIA+EH 
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHE 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSN------------------ 240
           LDWD +ST ++  K+HEDLL GPK   G +    P + +                     
Sbjct: 181 LDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDSEY 240

Query: 241 -----PSTPYGALHPVVSPVEKQEPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDT 300
                P  P   L P         P+   +     +++ D+    +N  V+ + ASK D 
Sbjct: 241 DILDFPEVPNVLLRPTPGATSVNAPDAAKS-ASYEHTSHDLPFDSENAGVE-KTASKRDE 300

Query: 301 RP---------------------------PP----------------------------W 327
            P                           PP                             
Sbjct: 301 HPAKASKTVVEGQQSSPILMESFEKKNYSPPSIDAVGPIPTKESGASRDTPRKISDGDLQ 360

BLAST of Cp4.1LG01g17770 vs. TAIR 10
Match: AT1G25420.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.0e-65
Identity = 141/248 (56.85%), Postives = 172/248 (69.35%), Query Frame = 0

Query: 70  NIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLPDLLQLKNLF 129
           N+WAAYEILELFCEF+LARVPI+ES+KECP ELREAIASIIFAAPRCS++PDLLQ+KNLF
Sbjct: 2   NLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLF 61

Query: 130 AVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTA 189
             KYGKEF+   SELRPDSGVNR+IIEKL+ ++P G  +LK++KEIA+E+ L+WDSS+T 
Sbjct: 62  GTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATE 121

Query: 190 SEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQEPECHHTFT 249
           +EF K+HEDLLGG K +H           S S PS        V   VE    E    F 
Sbjct: 122 AEFMKSHEDLLGGAKQIH------RQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 181

Query: 250 RI--SNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATAAARVAAE 309
           ++   N  S   P  K   +     +  DTR    DV+E ARAA+ASA+RATAAAR AA+
Sbjct: 182 KLQAQNPVSKSMPSSK---LTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQ 240

Query: 310 LANVNLGA 316
           L NV+ GA
Sbjct: 242 LVNVSYGA 240

BLAST of Cp4.1LG01g17770 vs. TAIR 10
Match: AT1G25420.3 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.0e-65
Identity = 141/248 (56.85%), Postives = 172/248 (69.35%), Query Frame = 0

Query: 70  NIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLPDLLQLKNLF 129
           N+WAAYEILELFCEF+LARVPI+ES+KECP ELREAIASIIFAAPRCS++PDLLQ+KNLF
Sbjct: 2   NLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLF 61

Query: 130 AVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTA 189
             KYGKEF+   SELRPDSGVNR+IIEKL+ ++P G  +LK++KEIA+E+ L+WDSS+T 
Sbjct: 62  GTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATE 121

Query: 190 SEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQEPECHHTFT 249
           +EF K+HEDLLGG K +H           S S PS        V   VE    E    F 
Sbjct: 122 AEFMKSHEDLLGGAKQIH------RQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 181

Query: 250 RI--SNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATAAARVAAE 309
           ++   N  S   P  K   +     +  DTR    DV+E ARAA+ASA+RATAAAR AA+
Sbjct: 182 KLQAQNPVSKSMPSSK---LTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQ 240

Query: 310 LANVNLGA 316
           L NV+ GA
Sbjct: 242 LVNVSYGA 240

BLAST of Cp4.1LG01g17770 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 241.9 bits (616), Expect = 7.2e-64
Identity = 164/434 (37.79%), Postives = 220/434 (50.69%), Query Frame = 0

Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60
           MS+L+  FN+G  A KCKT L L I RIKL++N+R+ Q+K MR+EIA+ L+TGQE  ARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEH+IRE+ + AA EILELFCE +  R+PIIE+Q+ECP++L+EAI+S+ FAAPRCSDL 
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNR---------------------------- 180
           +L Q++ LF  KYGKEFV+A SEL+PDSGVNR                            
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNS 180

Query: 181 --SIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAA 240
              ++E L+V AP  E KLK++KEIA+EH LDWD +ST ++  K+HEDLL GPK   G +
Sbjct: 181 ILQLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGS 240

Query: 241 ASQAPSQYSSSN-----------------------PSTPYGALHPVVSPVEKQEPECHHT 300
               P + +                          P  P   L P         P+   +
Sbjct: 241 KLPLPEEQNEKTNLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPTPGATSVNAPDAAKS 300

Query: 301 FTRISNSASDIKPGPKNFDVKPEVASKLDTRP---------------------------P 327
                +++ D+    +N  V+ + ASK D  P                           P
Sbjct: 301 -ASYEHTSHDLPFDSENAGVE-KTASKRDEHPAKASKTVVEGQQSSPILMESFEKKNYSP 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3ZBV11.4e-2437.36IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
P539904.2e-2437.36IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX004.2e-2437.36IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z64.2e-2437.36IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Q5R6G82.7e-2336.81IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023538831.19.66e-229100.00IST1 homolog isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6601778.12.00e-22197.86IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022921725.14.71e-22097.55IST1 homolog isoform X1 [Cucurbita moschata][more]
XP_022971809.18.76e-21897.55IST1 homolog isoform X1 [Cucurbita maxima][more]
KAG7032495.11.47e-21794.12IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1E6L92.28e-22097.55IST1 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1[more]
A0A6J1I9L34.24e-21897.55IST1 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1[more]
A0A6J1DR139.32e-19286.59IST1 homolog OS=Momordica charantia OX=3673 GN=LOC111022352 PE=3 SV=1[more]
A0A6J1E1A64.29e-19097.20IST1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1[more]
A0A6J1I6R96.84e-18997.55IST1 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G25420.14.1e-9962.46Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.21.3e-6840.59Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.21.0e-6556.85Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.31.0e-6556.85Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.17.2e-6437.79Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 18..181
e-value: 1.6E-62
score: 210.3
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 14..310
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 5..192
e-value: 4.5E-76
score: 256.6
NoneNo IPR availablePANTHERPTHR12161:SF13REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 14..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g17770.1Cp4.1LG01g17770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport