CsGy4G011010 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy4G011010 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 6.3e-113
Identity = 221/435 (50.80%), Postives = 300/435 (68.97%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           +DQ ++ P +FLCPIS  IMKDPV V++GITYDRESIEKWL S K N+CPV+  V++   
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLCRLR 123
           +TPNHTLRR+IQ+WCTLNAS G+ERIPTPKPP+ + ++ +++  ++ S  +Q   L RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIATANESNKRCMESAGVVEFLAGVVCNN-----------STTNMEYGLEDYAFD---DM 183
            I + N +NKRC+E+A V EFLA +V N+           S++N+    +    +   D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 SANSADEALLILHKLQVSEPSLKFLLSNNGGI-FVSTLTKILQNRSYSSRSYSVMLLNSM 243
           S +  DEAL +L+ L  SE +LK LL+N  G   V TLTKI+Q   Y SR+Y+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQIQNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVP 303
            EVAD +QI  L  + F E+IQIL DQIS +A++SA+++L+      RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGS 363
           ++I+LL+D   S  +R  EM +V+LD+LC CA+GRAE LNH A IAVVSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTW 423
           E+AV +L SV +F  + S++QEM+++GVV KLC VLQ V  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVW 420

BLAST of CsGy4G011010 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 5.5e-109
Identity = 210/421 (49.88%), Postives = 292/421 (69.36%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           +D+ ++ PP+FLCPIS +IMKDPV V++GITYDR+SIEKWL + K N+CPV+   ++   
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLCRLR 123
           +TPNHTLRR+IQ+WCTLNAS GVERIPTP+PP+ + ++ +++  +  S  +Q   L RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLILHK 183
            I + N +NKRC+E+AGV EFLA +V N               D  + +  DEAL +L+ 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQVSEPSLKFLLSN-NGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTA 243
           L+ SE  LK LL+N      V +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q   L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSEN 303
           + FTE++QIL D+IS++A+K+A+ +L+ +    RNR KAV+AG + V+I+LL+D   +  
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAKFS 363
           +R  EM +V+LDLLC CA+GRAE LNH A IAVV KKILRVS   S++AV +L SV +F 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 GSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAY 423
            + +++ EM+++GVV KLC VLQ V  G K KEKAKE+LKLH R W++S CLP  +  AY
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

BLAST of CsGy4G011010 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.9e-61
Identity = 169/459 (36.82%), Postives = 245/459 (53.38%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           M + ++ ++ P YF+CPIS +IMKDPVT  SGITYDR++I KWL   K  +CPV+   L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 61  -HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQ---- 120
               +TPNH LRR+IQ WC  N ++GV RI TP+ P  +  VV  +   NL  F Q    
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEI--KNLKKFGQEALG 120

Query: 121 -KNSLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSA 180
            + +L +L  +A  + +N+R M   GV + L   V    T+  E G              
Sbjct: 121 REETLQKLEVLA-MDGNNRRLMCECGVHKSLILFVV-KCTSEDEDGRR-------RIKGL 180

Query: 181 DEALLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQ 240
           DE+L +LH + +     K +L  N  + + +LT +L    + S++Y+++LL ++ E    
Sbjct: 181 DESLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSS 240

Query: 241 IQIQNLTADFFTEIIQILKDQIS-----------------------------------KQ 300
             ++ L  + F  II  LKD ++                                   KQ
Sbjct: 241 HIVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQ 300

Query: 301 ASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDLLCSC 360
           A  +AL +L+  SS  RNR   V  GAV  LI+  L++  +  KR+ E++L +L  LC C
Sbjct: 301 AVTAALMILLETSSWSRNRSLLVDLGAVSELIE--LEISYTGEKRITELMLGVLSRLCCC 360

Query: 361 ADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAKFSGSQSVVQEMVRIGVVTK 419
           A+GRAE+L H  GIAVV+K++LRVS    ++A+ IL +V+KFS    VV+EMV +G V K
Sbjct: 361 ANGRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEK 420

BLAST of CsGy4G011010 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 9.5e-45
Identity = 139/416 (33.41%), Postives = 218/416 (52.40%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           LD  +  P +F CPIS ++M+DPVTV +G TYDR SIE W+    + TCPV+   LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHA------NLSPFSQKN 123
           + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A      ++S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 183
           +L RLR  A  ++ N+  + +    E L  ++ + +T               S+    E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 243
           L +L  L ++EP+    +S++ G  V  LT++L + S  +R  +  L+  +        +
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 QNLTAD---FFTEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLID- 303
           +   ++    F  ++ +L++ I S++A K  +K L A+ S    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAV 363
           L  D D  + +R     L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 LAADFDRCDTER----ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAA 367

Query: 364 GILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTW 409
           G L  +A  +  +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 368 GAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of CsGy4G011010 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 8.9e-43
Identity = 136/424 (32.08%), Postives = 218/424 (51.42%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           LD  +  P +F CPIS  +M DPVT+++G TYDR SI+ W ++  + TCPV+ + LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSW-IAMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHA------NLSPFSQKN 123
           + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A      ++S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 183
           ++ RLR +A  +E N+  +      E L  ++             D     +S+    E+
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL-----------FADIETTSLSSELVSES 187

Query: 184 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 243
           L +L  L ++E   + + S+     V  +T++L + S   R  +  L+  +   A  + +
Sbjct: 188 LALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL 247

Query: 244 QNLTA---DFFTEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLID- 303
           + + +     F  ++ +LK+ I S++A K  +K + A+    + R  A+ AGA  +LID 
Sbjct: 248 KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDR 307

Query: 304 LLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAV 363
           L  D D  + +R     L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 LAADFDRCDTER----GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSS 417
           G L  +A  +  +    E    G+VT+L  ++Q      +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

BLAST of CsGy4G011010 vs. NCBI nr
Match: XP_011653475.1 (E3 ubiquitin-protein ligase PUB22 [Cucumis sativus] >KGN53961.1 hypothetical protein Csa_021573 [Cucumis sativus])

HSP 1 Score: 810 bits (2092), Expect = 3.01e-295
Identity = 422/424 (99.53%), Postives = 423/424 (99.76%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS
Sbjct: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60

Query: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120
           HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC
Sbjct: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120

Query: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 180
           RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI
Sbjct: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 180

Query: 181 LHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNL 240
           LHKLQVSE SLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMF+VADQIQIQNL
Sbjct: 181 LHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQIQNL 240

Query: 241 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 300
           TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS
Sbjct: 241 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 300

Query: 301 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 360
           ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK
Sbjct: 301 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 360

Query: 361 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 420
           FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS
Sbjct: 361 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 420

Query: 421 AYPQ 424
           AYPQ
Sbjct: 421 AYPQ 424

BLAST of CsGy4G011010 vs. NCBI nr
Match: XP_008441961.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo] >KAA0060143.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa] >TYK08456.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa])

HSP 1 Score: 758 bits (1956), Expect = 1.72e-274
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0

Query: 1   MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 60
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 120
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 180
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180

Query: 181 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240
           L+ILHKLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI
Sbjct: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240

Query: 241 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 301 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 360
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360

Query: 361 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 421 LRSAYPQ 424
           LRSAYPQ
Sbjct: 421 LRSAYPQ 426

BLAST of CsGy4G011010 vs. NCBI nr
Match: XP_038882772.1 (E3 ubiquitin-protein ligase PUB22-like [Benincasa hispida])

HSP 1 Score: 684 bits (1764), Expect = 2.79e-245
Identity = 363/425 (85.41%), Postives = 391/425 (92.00%), Query Frame = 0

Query: 1   MAELDQ-VLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVL 60
           MAELDQ V+DPPP+FLCPIS QIMKDPVT+ASGITYDRE+IEKWL S KH TCPVS +VL
Sbjct: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60

Query: 61  SHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSL 120
             F ITPNHTLRRVIQAWCT NA+ GVERIPTPKPPVDR+QVVRILA A LSPFSQ+N L
Sbjct: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120

Query: 121 CRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALL 180
            RLRSIA ANE+NKRCME+AGVVEFLAG+VC+ STTN+E GLEDYAFDDMSAN  DEAL 
Sbjct: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCS-STTNVESGLEDYAFDDMSANLGDEALS 180

Query: 181 ILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQN 240
           IL+KLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSR+YS+MLLNSMFEVADQIQI N
Sbjct: 181 ILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILN 240

Query: 241 LTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDS 300
           LT DFF EI+QILKDQISKQASKSALKLLI V SS RNRVKAV+AGAVPVL+DLLLDLDS
Sbjct: 241 LTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDS 300

Query: 301 SENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVA 360
           SE KRL EMILV+LDLLC CADGRA LLNHAAGIAVVSKKILRVSS+GSEKAVGIL SVA
Sbjct: 301 SE-KRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360

Query: 361 KFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLR 420
           +FS SQSV+QEMVR+GVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS+LR
Sbjct: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SAYPQ 424
           SAYPQ
Sbjct: 421 SAYPQ 423

BLAST of CsGy4G011010 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 650 bits (1676), Expect = 6.69e-232
Identity = 341/425 (80.24%), Postives = 380/425 (89.41%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIE+WL S +H TCPV+ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120
            F ITPNHT+RRVIQAWCT+NA KG+ERIPTPKPP+D +QVVRILA A LSPFSQ+N L 
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 180
           RLRSIA A+ESNKRCME+AG VEFLAG+VCN+  TN+E+ LEDYA+  DD  AN  DEAL
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNS--TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 181 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 301 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 360
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSI 360

Query: 361 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420
           A+FS S+SVVQEM+R+GVVTKLCF+LQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 421 RSAYP 423
           RS++P
Sbjct: 421 RSSFP 422

BLAST of CsGy4G011010 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647 bits (1668), Expect = 1.10e-230
Identity = 341/425 (80.24%), Postives = 378/425 (88.94%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIEKWL S +H TCPV+ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60

Query: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120
            F ITPNHTLRRVIQAWCT+NA KG+ERIPTPKPPVD +QVVRILA A LSPFSQ+N L 
Sbjct: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 180
           RLRSIA  +ESNKRCME+AG VEFLAG+VCN+  TN+E+  EDYA+  DD  AN  DEAL
Sbjct: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNS--TNVEFSSEDYAYLIDDQFANLGDEAL 180

Query: 181 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPV+IDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLD 300

Query: 301 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 360
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSI 360

Query: 361 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420
           A+FS S+SVVQEM+R+GVVTKLCF+LQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 421 RSAYP 423
           RS++P
Sbjct: 421 RSSFP 422

BLAST of CsGy4G011010 vs. ExPASy TrEMBL
Match: A0A0A0L1F5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE=4 SV=1)

HSP 1 Score: 810 bits (2092), Expect = 1.46e-295
Identity = 422/424 (99.53%), Postives = 423/424 (99.76%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS
Sbjct: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60

Query: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120
           HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC
Sbjct: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120

Query: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 180
           RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI
Sbjct: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 180

Query: 181 LHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNL 240
           LHKLQVSE SLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMF+VADQIQIQNL
Sbjct: 181 LHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQIQNL 240

Query: 241 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 300
           TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS
Sbjct: 241 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 300

Query: 301 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 360
           ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK
Sbjct: 301 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 360

Query: 361 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 420
           FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS
Sbjct: 361 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 420

Query: 421 AYPQ 424
           AYPQ
Sbjct: 421 AYPQ 424

BLAST of CsGy4G011010 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 758 bits (1956), Expect = 8.31e-275
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0

Query: 1   MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 60
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 120
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 180
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180

Query: 181 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240
           L+ILHKLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI
Sbjct: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240

Query: 241 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 301 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 360
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360

Query: 361 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 421 LRSAYPQ 424
           LRSAYPQ
Sbjct: 421 LRSAYPQ 426

BLAST of CsGy4G011010 vs. ExPASy TrEMBL
Match: A0A1S3B4K2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 758 bits (1956), Expect = 8.31e-275
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0

Query: 1   MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 60
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 120
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 180
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180

Query: 181 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240
           L+ILHKLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI
Sbjct: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240

Query: 241 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 301 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 360
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360

Query: 361 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 421 LRSAYPQ 424
           LRSAYPQ
Sbjct: 421 LRSAYPQ 426

BLAST of CsGy4G011010 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 650 bits (1676), Expect = 3.24e-232
Identity = 341/425 (80.24%), Postives = 380/425 (89.41%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIE+WL S +H TCPV+ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120
            F ITPNHT+RRVIQAWCT+NA KG+ERIPTPKPP+D +QVVRILA A LSPFSQ+N L 
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 180
           RLRSIA A+ESNKRCME+AG VEFLAG+VCN+  TN+E+ LEDYA+  DD  AN  DEAL
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNS--TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 181 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 301 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 360
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSI 360

Query: 361 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420
           A+FS S+SVVQEM+R+GVVTKLCF+LQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 421 RSAYP 423
           RS++P
Sbjct: 421 RSSFP 422

BLAST of CsGy4G011010 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 1.03e-228
Identity = 338/425 (79.53%), Postives = 378/425 (88.94%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIE+WL S +H TCP++ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120
           +F ITPNHTLRRVIQAWCT+NA KGVERIPTPKPPVD +QVVRILA A LS FSQ+N L 
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 180
           RL+SIA A+ESNKRCME+AG VEFLAG+VCN+  TN+E+ LEDYA+  DD  AN   EAL
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNS--TNVEFSLEDYAYLIDDQLANLGFEAL 180

Query: 181 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 301 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 360
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIA+VSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSI 360

Query: 361 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420
           A+FS S+SVVQEM+R+GVVTKLCF+LQV GAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 421 RSAYP 423
           RS++P
Sbjct: 421 RSSFP 422

BLAST of CsGy4G011010 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 409.1 bits (1050), Expect = 4.4e-114
Identity = 221/435 (50.80%), Postives = 300/435 (68.97%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           +DQ ++ P +FLCPIS  IMKDPV V++GITYDRESIEKWL S K N+CPV+  V++   
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLCRLR 123
           +TPNHTLRR+IQ+WCTLNAS G+ERIPTPKPP+ + ++ +++  ++ S  +Q   L RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIATANESNKRCMESAGVVEFLAGVVCNN-----------STTNMEYGLEDYAFD---DM 183
            I + N +NKRC+E+A V EFLA +V N+           S++N+    +    +   D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 SANSADEALLILHKLQVSEPSLKFLLSNNGGI-FVSTLTKILQNRSYSSRSYSVMLLNSM 243
           S +  DEAL +L+ L  SE +LK LL+N  G   V TLTKI+Q   Y SR+Y+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQIQNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVP 303
            EVAD +QI  L  + F E+IQIL DQIS +A++SA+++L+      RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGS 363
           ++I+LL+D   S  +R  EM +V+LD+LC CA+GRAE LNH A IAVVSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTW 423
           E+AV +L SV +F  + S++QEM+++GVV KLC VLQ V  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVW 420

BLAST of CsGy4G011010 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 396.0 bits (1016), Expect = 3.9e-110
Identity = 210/421 (49.88%), Postives = 292/421 (69.36%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           +D+ ++ PP+FLCPIS +IMKDPV V++GITYDR+SIEKWL + K N+CPV+   ++   
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLCRLR 123
           +TPNHTLRR+IQ+WCTLNAS GVERIPTP+PP+ + ++ +++  +  S  +Q   L RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLILHK 183
            I + N +NKRC+E+AGV EFLA +V N               D  + +  DEAL +L+ 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQVSEPSLKFLLSN-NGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTA 243
           L+ SE  LK LL+N      V +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q   L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSEN 303
           + FTE++QIL D+IS++A+K+A+ +L+ +    RNR KAV+AG + V+I+LL+D   +  
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAKFS 363
           +R  EM +V+LDLLC CA+GRAE LNH A IAVV KKILRVS   S++AV +L SV +F 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 GSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAY 423
            + +++ EM+++GVV KLC VLQ V  G K KEKAKE+LKLH R W++S CLP  +  AY
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

BLAST of CsGy4G011010 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-62
Identity = 169/459 (36.82%), Postives = 245/459 (53.38%), Query Frame = 0

Query: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60
           M + ++ ++ P YF+CPIS +IMKDPVT  SGITYDR++I KWL   K  +CPV+   L 
Sbjct: 15  MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 74

Query: 61  -HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQ---- 120
               +TPNH LRR+IQ WC  N ++GV RI TP+ P  +  VV  +   NL  F Q    
Sbjct: 75  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEI--KNLKKFGQEALG 134

Query: 121 -KNSLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSA 180
            + +L +L  +A  + +N+R M   GV + L   V    T+  E G              
Sbjct: 135 REETLQKLEVLA-MDGNNRRLMCECGVHKSLILFVV-KCTSEDEDGRR-------RIKGL 194

Query: 181 DEALLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQ 240
           DE+L +LH + +     K +L  N  + + +LT +L    + S++Y+++LL ++ E    
Sbjct: 195 DESLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSS 254

Query: 241 IQIQNLTADFFTEIIQILKDQIS-----------------------------------KQ 300
             ++ L  + F  II  LKD ++                                   KQ
Sbjct: 255 HIVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQ 314

Query: 301 ASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDLLCSC 360
           A  +AL +L+  SS  RNR   V  GAV  LI+  L++  +  KR+ E++L +L  LC C
Sbjct: 315 AVTAALMILLETSSWSRNRSLLVDLGAVSELIE--LEISYTGEKRITELMLGVLSRLCCC 374

Query: 361 ADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAKFSGSQSVVQEMVRIGVVTK 419
           A+GRAE+L H  GIAVV+K++LRVS    ++A+ IL +V+KFS    VV+EMV +G V K
Sbjct: 375 ANGRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEK 434

BLAST of CsGy4G011010 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 182.6 bits (462), Expect = 6.8e-46
Identity = 139/416 (33.41%), Postives = 218/416 (52.40%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           LD  +  P +F CPIS ++M+DPVTV +G TYDR SIE W+    + TCPV+   LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHA------NLSPFSQKN 123
           + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A      ++S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 183
           +L RLR  A  ++ N+  + +    E L  ++ + +T               S+    E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 243
           L +L  L ++EP+    +S++ G  V  LT++L + S  +R  +  L+  +        +
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 QNLTAD---FFTEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLID- 303
           +   ++    F  ++ +L++ I S++A K  +K L A+ S    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAV 363
           L  D D  + +R     L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 LAADFDRCDTER----ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAA 367

Query: 364 GILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTW 409
           G L  +A  +  +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 368 GAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of CsGy4G011010 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 176.0 bits (445), Expect = 6.3e-44
Identity = 136/424 (32.08%), Postives = 218/424 (51.42%), Query Frame = 0

Query: 4   LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 63
           LD  +  P +F CPIS  +M DPVT+++G TYDR SI+ W ++  + TCPV+ + LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSW-IAMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHA------NLSPFSQKN 123
           + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A      ++S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 183
           ++ RLR +A  +E N+  +      E L  ++             D     +S+    E+
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL-----------FADIETTSLSSELVSES 187

Query: 184 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 243
           L +L  L ++E   + + S+     V  +T++L + S   R  +  L+  +   A  + +
Sbjct: 188 LALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL 247

Query: 244 QNLTA---DFFTEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLID- 303
           + + +     F  ++ +LK+ I S++A K  +K + A+    + R  A+ AGA  +LID 
Sbjct: 248 KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDR 307

Query: 304 LLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAV 363
           L  D D  + +R     L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 LAADFDRCDTER----GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSS 417
           G L  +A  +  +    E    G+VT+L  ++Q      +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410