CsGy1G025350 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G025350
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionExonuclease domain-containing protein
LocationGy14Chr1: 24311659 .. 24313962 (-)
RNA-Seq ExpressionCsGy1G025350
SyntenyCsGy1G025350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTATTTATTAATTATTTCGGAAGAATAATTATAGAGAAAGCAACGAAGCTAAAGATTTCTCTTCCCACCTCCATGTCTCCCTAAAGCACCTCTCAATCTTCTTCCACGCCATTTACTCCCTTAACTTACGTTTTTCCTTTGGTTTTTAAATGCAGTATAATCCTCCCTCAGATTTCATTTCAGGCAAGTTCCTTTTCTCTTTCTCTCGCTTTGTAATTTCCATGTTTTTGCATTCAAAGATTGCTTTAGATTGCCGAGAAGTGGGATGATGTTGTACTCTTTTGAAATCTGAAGGTAAATAGTTTGAAGTTGTGTGAGAATGAAATGGGTCGATTTCTGTTTCTGCTATAAACCCTTTTTTTTGTTTTTTTAATCTAATGCAGTAGTGCTTCATTTGGACGTTTTTAAGTGTCACCGATCGATTGGTTTGCGTTGTTTGATTTTTAGTAATGAATGTTTGGGTTTCTGTTTGTGGGGTATTGGCGAAAGATTGGAGATGGCGGGAGTTGGGAGTGTTCTAGGCCAAATCGGTTTGATTACAATGTGTTTGTTAAAATGTCTGAATAAGATCGTTGAAAATAAATTATTATTATTATTTTTTTTTCTTTTTTTTTGTCTTTGACGTAATTGTGCTTATACTTTTGAGATCCATCATATGGAGCCCTTAACTTTTTTGTTTTGTTTATTATTTTCGTTATTCTGTATGATAATCAATGTGGAGAATTTAAAGTTGATCCCTTTTCTGTTTCTTGATCTCTGATATGGGGTTATACATTGGTTGTCACACCCCGTTATCTACAGGTTGAAGAAAATTGCTTTCGGTTGCTGGATTTTGTTGGGTTTTCTGTCTAGATTTCCCCTTGTTGTGCTGTGAATTCGAATTTTAACAGCTCCAATGTGCCTTTCAATCTTCCGATTTCCGAAATATGGAGTCCCTGTTTTAGCTAACCTTTGGAGGGAAAACTTTCATCCCTTGAACAGGAATCATGGTCATAATTGTTGGTATGATCAGCTTTGTTTTAGGATCTATAACCTTGAAGGAGGGCGGAACAAGAGATGGACCAGAAAATCTATTACTACCAAAGCGGGGGAGAAGGCAAAGACCAACCCTAGCAGTAAACCCACCAATATCAGGAATGAAATCTTGCAGGAATCGATGTTAGCAAGCTGTACAGTTAACATTAACAAGTCCGAGAAAAGTGAAACTCAGAAGCTTCAATATTGTGATATTCAACCAACAATTATTAAGAGCAAGGAATTCGCTCACCTGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAAACCTCGATAGAATAGTGGAAATTGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTTCAAACATTAATAAATCCACAGTGCTATATTACAAATTCAAATATACATGGCATTTCAAACCGTATGGTCGATAGTCCCAGTGTTCCAAGGTATTGAAACGTTCATAGTAGTCTCATTTCTCTTATATCTTCCATTCAGTTCACTGCTAGTTACAGTCATTTATCAATCATATATTTTTATACATGTTCAAACGCTCCTTTTCTTGTTCATATAGGATGCAGGAACTTATTCCAATTATATTACAATTTGTCAAAAGCCGTCAGAAACCTGGTGGTTATGTATTATTGGTAGCTCATAATGCTCGTACCTTCGACGTACCCTTTTTACTCAGTGAATTCAGCCGTTACTCCGTTGATATTCCTCTAAATTGGCTATTTTTCGATACTATGACGTTAGCCCGTCAACTGATGAAGTTATCCGGTTAGTTTTCCCTTTCCCCATATTATCGTTTTCTAGTTTCTCTGTGAATAAAAGGATTAGAGTGATGTAATGTAATATTGTTGATGGCAATATCATGATCAGATTCGAAGATTACAAAAATAACATTACAAGCTCTTGGCGAGTATTACGGTATAAAACTGGATGGTAAAGCACATCGAGCATTGTCAGACGTGAGATTGTTGTCATCGATTCTTCAGAGGCTTACTTTCGACCTCAAGCTAGATATCTCGGATCTCGTTGAAAGAGCTTTCACTCCTTTAGATCTGATTAATAAGAAGAAAAAATAGTTTGTTGGAGTTTTTGTTGTAATGTTCTTTACTAATTGTTGTAACTTTTGTTCATTCTTATTTGAATAACTTTTGGTCATTTTATTTACCCTCATTAGATCCTTCTTGATGTCCATATTCTCAAGAAACGGGTGGATGAATTGAAATGTCAGGACATAGTTGTTTACAAAGAAATATGAAAGGAAGATAGTTTGATG

mRNA sequence

CCTATTTATTAATTATTTCGGAAGAATAATTATAGAGAAAGCAACGAAGCTAAAGATTTCTCTTCCCACCTCCATGTCTCCCTAAAGCACCTCTCAATCTTCTTCCACGCCATTTACTCCCTTAACTTACGTTTTTCCTTTGGTTTTTAAATGCAGTATAATCCTCCCTCAGATTTCATTTCAGGTTGAAGAAAATTGCTTTCGGTTGCTGGATTTTGTTGGGTTTTCTGTCTAGATTTCCCCTTGTTGTGCTGTGAATTCGAATTTTAACAGCTCCAATGTGCCTTTCAATCTTCCGATTTCCGAAATATGGAGTCCCTGTTTTAGCTAACCTTTGGAGGGAAAACTTTCATCCCTTGAACAGGAATCATGGTCATAATTGTTGGTATGATCAGCTTTGTTTTAGGATCTATAACCTTGAAGGAGGGCGGAACAAGAGATGGACCAGAAAATCTATTACTACCAAAGCGGGGGAGAAGGCAAAGACCAACCCTAGCAGTAAACCCACCAATATCAGGAATGAAATCTTGCAGGAATCGATGTTAGCAAGCTGTACAGTTAACATTAACAAGTCCGAGAAAAGTGAAACTCAGAAGCTTCAATATTGTGATATTCAACCAACAATTATTAAGAGCAAGGAATTCGCTCACCTGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAAACCTCGATAGAATAGTGGAAATTGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTTCAAACATTAATAAATCCACAGTGCTATATTACAAATTCAAATATACATGGCATTTCAAACCGTATGGTCGATAGTCCCAGTGTTCCAAGGATGCAGGAACTTATTCCAATTATATTACAATTTGTCAAAAGCCGTCAGAAACCTGGTGGTTATGTATTATTGGTAGCTCATAATGCTCGTACCTTCGACGTACCCTTTTTACTCAGTGAATTCAGCCGTTACTCCGTTGATATTCCTCTAAATTGGCTATTTTTCGATACTATGACGTTAGCCCGTCAACTGATGAAGTTATCCGATTCGAAGATTACAAAAATAACATTACAAGCTCTTGGCGAGTATTACGGTATAAAACTGGATGGTAAAGCACATCGAGCATTGTCAGACGTGAGATTGTTGTCATCGATTCTTCAGAGGCTTACTTTCGACCTCAAGCTAGATATCTCGGATCTCGTTGAAAGAGCTTTCACTCCTTTAGATCTGATTAATAAGAAGAAAAAATAGTTTGTTGGAGTTTTTGTTGTAATGTTCTTTACTAATTGTTGTAACTTTTGTTCATTCTTATTTGAATAACTTTTGGTCATTTTATTTACCCTCATTAGATCCTTCTTGATGTCCATATTCTCAAGAAACGGGTGGATGAATTGAAATGTCAGGACATAGTTGTTTACAAAGAAATATGAAAGGAAGATAGTTTGATG

Coding sequence (CDS)

ATGTGCCTTTCAATCTTCCGATTTCCGAAATATGGAGTCCCTGTTTTAGCTAACCTTTGGAGGGAAAACTTTCATCCCTTGAACAGGAATCATGGTCATAATTGTTGGTATGATCAGCTTTGTTTTAGGATCTATAACCTTGAAGGAGGGCGGAACAAGAGATGGACCAGAAAATCTATTACTACCAAAGCGGGGGAGAAGGCAAAGACCAACCCTAGCAGTAAACCCACCAATATCAGGAATGAAATCTTGCAGGAATCGATGTTAGCAAGCTGTACAGTTAACATTAACAAGTCCGAGAAAAGTGAAACTCAGAAGCTTCAATATTGTGATATTCAACCAACAATTATTAAGAGCAAGGAATTCGCTCACCTGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAAACCTCGATAGAATAGTGGAAATTGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTTCAAACATTAATAAATCCACAGTGCTATATTACAAATTCAAATATACATGGCATTTCAAACCGTATGGTCGATAGTCCCAGTGTTCCAAGGATGCAGGAACTTATTCCAATTATATTACAATTTGTCAAAAGCCGTCAGAAACCTGGTGGTTATGTATTATTGGTAGCTCATAATGCTCGTACCTTCGACGTACCCTTTTTACTCAGTGAATTCAGCCGTTACTCCGTTGATATTCCTCTAAATTGGCTATTTTTCGATACTATGACGTTAGCCCGTCAACTGATGAAGTTATCCGATTCGAAGATTACAAAAATAACATTACAAGCTCTTGGCGAGTATTACGGTATAAAACTGGATGGTAAAGCACATCGAGCATTGTCAGACGTGAGATTGTTGTCATCGATTCTTCAGAGGCTTACTTTCGACCTCAAGCTAGATATCTCGGATCTCGTTGAAAGAGCTTTCACTCCTTTAGATCTGATTAATAAGAAGAAAAAATAG

Protein sequence

MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSITTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSKEFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGISNRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIPLNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRLTFDLKLDISDLVERAFTPLDLINKKKK*
Homology
BLAST of CsGy1G025350 vs. ExPASy Swiss-Prot
Match: Q682U6 (Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DPD1 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.1e-60
Identity = 138/326 (42.33%), Postives = 199/326 (61.04%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC+SI +  +  +    +   E  H   +N         L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKNSSS---LKLLDVRASSVDG--KARWIRRNV 60

Query: 61  -TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINK--SEKSETQKLQYCDIQPTII 120
            TT  G ++ T               +S +   TV + +   E+S T+   + ++Q  + 
Sbjct: 61  STTTQGSRSNT---------------KSSVLGGTVPVTRIIDEESRTKVQPFGNLQQRLA 120

Query: 121 KSKEFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCY-ITNSNI 180
           + K+ + L+TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    ITN++I
Sbjct: 121 QDKDLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHI 180

Query: 181 HGISNRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYS 240
           HGI N MV  P VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S
Sbjct: 181 HGIRNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCS 240

Query: 241 VDIPLNWLFFDTMTLARQLMKLSDSKI-TKITLQALGEYYGIKLDGKAHRALSDVRLLSS 300
            +IP NWL  D++ LAR+ MK  +  +    +L+AL +YY +  +G AHRALSDV LLS 
Sbjct: 241 YEIPHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSK 300

Query: 301 ILQRLTFDLKLDISDLVERAFTPLDL 322
           + Q+LT DLKL +SDLV R  T  D+
Sbjct: 301 VFQKLTIDLKLSLSDLVLRCHTASDI 306

BLAST of CsGy1G025350 vs. ExPASy Swiss-Prot
Match: Q8RA32 (DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=polC PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 4.1e-11
Identity = 56/175 (32.00%), Postives = 89/175 (50.86%), Query Frame = 0

Query: 129 ITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLINPQCYITN--SNIHGISNRMVD 188
           + FDIETTG S   D I+EI    +   +   TF+T +NPQ  I++  + + GI   MV 
Sbjct: 389 VVFDIETTGLSNINDEIIEIGAVKIKNKKIVDTFETFVNPQIPISSFITKLTGIDESMV- 448

Query: 189 SPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIPLNWLF 248
               P ++E++P  L+F K         +LVAHNA  FDV F+ S+  +  + +    L 
Sbjct: 449 -KDAPLIEEVLPKFLEFAKG-------AVLVAHNA-NFDVSFIKSKAKKLGLTVENTVL- 508

Query: 249 FDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 301
            DT+ L+R L +     +    L  L E++ +KL    HRA+ D +  + I  ++
Sbjct: 509 -DTLELSRHLYQ----DLKNYKLDTLAEFFEVKL-LHHHRAVEDAKATAEIFIKM 546

BLAST of CsGy1G025350 vs. ExPASy Swiss-Prot
Match: Q8XJR3 (DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=polC PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 6.9e-11
Identity = 64/203 (31.53%), Postives = 99/203 (48.77%), Query Frame = 0

Query: 114 PTIIKSKEFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLINPQCYIT 173
           P +I   E +     + FDIETTGFS   D+I+EI    L  GE   +F T ++P+  I 
Sbjct: 421 PIVINGHEESFDDEYVVFDIETTGFSSKNDKIIEIGAVKLKDGEIVDSFSTFVDPKVNIP 480

Query: 174 N--SNIHGISNRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLS 233
              + +  I+  MV+    P + E++P  ++FV       G  +LVAHNA  FDV F+  
Sbjct: 481 YKITELTSITQNMVNGQ--PTIDEVLPKFMEFV-------GNSVLVAHNA-AFDVGFIKK 540

Query: 234 EFSRYSVDIPLNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDV 293
                 +   L     DT+ LAR L       + K+ L  + ++ GI L+   HRA+ D 
Sbjct: 541 NL--MDMGKTLKNPVMDTVPLARYLY----PDLKKVKLNLVAKHLGISLENH-HRAVDDA 600

Query: 294 RLLSSILQRLTFDLK--LDISDL 312
           +  + IL+     +K  +DI D+
Sbjct: 601 KATAEILKFSFKKMKEEMDIHDV 606

BLAST of CsGy1G025350 vs. ExPASy Swiss-Prot
Match: Q895K2 (DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=polC PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.4e-10
Identity = 60/200 (30.00%), Postives = 96/200 (48.00%), Query Frame = 0

Query: 129 ITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLINPQCYITNSNIH--GISNRMVD 188
           + FDIETTGFS   D+I+EI    +  G+    + T +NP+  I    I   GI + MV 
Sbjct: 414 VVFDIETTGFSNKNDKIIEIGAVKIKEGKVVDKYSTFVNPERIIPEKIIELTGIHDYMV- 473

Query: 189 SPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIPLNWLF 248
               P+++E++P  + F++         +LVAHNA  FDV F+      +   I  N   
Sbjct: 474 -KDAPKIEEVLPKFIDFIEDS-------ILVAHNA-NFDVSFIKKNCKDFG--IHFNNPI 533

Query: 249 FDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRLTFDLK 308
            DT+ L + L      ++ +  L  + ++ GI L    HRA+ D +    IL +   DLK
Sbjct: 534 LDTIPLCKFLY----PELKRYKLNVVAKHLGIPLLNH-HRAVEDAKTTGDILLKAFEDLK 593

Query: 309 ----LDISDLVERAFTPLDL 322
               ++++ L E  F   D+
Sbjct: 594 DKEIMNLNMLNEEYFKNQDI 596

BLAST of CsGy1G025350 vs. ExPASy Swiss-Prot
Match: Q8EQU6 (DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=polC PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.2e-08
Identity = 58/181 (32.04%), Postives = 89/181 (49.17%), Query Frame = 0

Query: 127 TVITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLINPQCYITNSNIH--GISNRM 186
           T + FD+ETTG S   D I+E+A   + GGE    F++  NP   ++ + I   GI++ M
Sbjct: 413 TFVVFDVETTGLSAVYDTIIELAAVKIKGGEIVDRFESFANPHHALSQTTIDLTGITDDM 472

Query: 187 VDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIPLNW 246
           V+           P +   +K      G  +LVAHNA +FD+ FL   F R   +   N 
Sbjct: 473 VND---------APEVADVLKDFHTWMGNDILVAHNA-SFDMGFLNQGFQRIDYEKASNP 532

Query: 247 LFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVR----LLSSILQR 301
           +  DT+ LAR L+     ++    L  L ++  I+L  + HRA+ D      LL  ++QR
Sbjct: 533 V-VDTLELARFLL----PELRNHRLNTLCKHLDIELT-QHHRAIYDAEATGYLLWKLVQR 577

BLAST of CsGy1G025350 vs. NCBI nr
Match: XP_004135590.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucumis sativus] >KGN66030.1 hypothetical protein Csa_007467 [Cucumis sativus])

HSP 1 Score: 652 bits (1682), Expect = 4.85e-236
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP
Sbjct: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKLDISDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of CsGy1G025350 vs. NCBI nr
Match: ALG62680.1 (exonuclease DPD1 [Cucumis sativus] >ALG62681.1 exonuclease DPD1 [Cucumis sativus])

HSP 1 Score: 648 bits (1672), Expect = 1.62e-234
Identity = 325/327 (99.39%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAGEKAKTNPSSKPTNIRNEILQES+LASCTVNINKSEKSETQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESVLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           NRMVDSPSVP MQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP
Sbjct: 181 NRMVDSPSVPSMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKLDISDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of CsGy1G025350 vs. NCBI nr
Match: XP_008450585.1 (PREDICTED: exonuclease DPD1, chloroplastic/mitochondrial [Cucumis melo] >XP_016900923.1 PREDICTED: exonuclease DPD1, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 585 bits (1509), Expect = 1.13e-209
Identity = 292/327 (89.30%), Postives = 308/327 (94.19%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC SIFRFPK+GVP+LANLWRENFHPLNRNHGH+CWYDQLCFRIYNLEGG+NKRWTRKSI
Sbjct: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKPTNI NEILQESM ASCTVN+NKSEKS TQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           EF H VTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLI+PQCYITNS+IHGI+
Sbjct: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           N MV S  VPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLL EFSRYS+DIP
Sbjct: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
            +WLF DTMTLARQLMKLSDSKITKI+L+AL EYYGI++DG+AHRAL DV  LS ILQRL
Sbjct: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKLD+SDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327

BLAST of CsGy1G025350 vs. NCBI nr
Match: XP_022968752.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 515 bits (1327), Expect = 7.14e-182
Identity = 261/327 (79.82%), Postives = 285/327 (87.16%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC SI R PK+GVP LANLWRENFHPLNRN+GH+ WYDQL FRIYNLEGG++KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKP+NIRNEIL+ES+  SCTV+INKSEK  TQKLQ C+IQ TIIK K
Sbjct: 66  TTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQETIIKDK 125

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           E A  VTVITFDIETTGFSR LDRIVEIA QDLSGGENSTFQTLINP+CYI N+ +HGI+
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           +RMV  P VPRM+ELIPI+LQFVKSRQKP GYVLLVAHNAR FDVPFLL EFSR SVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRCSVDIP 245

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFD + LARQLMK +DSKITKI+LQAL EYY I L G AHRA+SDVR LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKL I DL+ER+FT  DLINKKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKK 332

BLAST of CsGy1G025350 vs. NCBI nr
Match: KAG6590475.1 (Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024006.1 Exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 513 bits (1320), Expect = 8.31e-181
Identity = 257/326 (78.83%), Postives = 286/326 (87.73%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC SI R PK+GVP LANLWRENFHPLNRN+GH+ WYDQL FRIYNLEGG++KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           +TKAG KAKT+PSSKP+NIRNEIL+ES+  SCTV+INKSEK+ TQKLQ C+IQ TI+K K
Sbjct: 66  STKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQETIVKDK 125

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           E A  VTVITFDIETTGFSR LDRIVEIA QDLSGGENSTFQTLINP+CYI N+ +HGI+
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           +RMV  P VPRM+ELIPI+LQF+KSRQKP GYVLLVAHNARTFDVPFLL EFSR SVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRCSVDIP 245

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFD + LARQLMK +DSKITKI+LQAL EYY I L G AHRA+SDVR LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLDISDLVERAFTPLDLINKKK 326
           TFDLKL I DL+ER+FT  DLINKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of CsGy1G025350 vs. ExPASy TrEMBL
Match: A0A0A0LVY3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080 PE=4 SV=1)

HSP 1 Score: 652 bits (1682), Expect = 2.35e-236
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP
Sbjct: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKLDISDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of CsGy1G025350 vs. ExPASy TrEMBL
Match: A0A0N9LRH7 (Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1)

HSP 1 Score: 648 bits (1672), Expect = 7.85e-235
Identity = 325/327 (99.39%), Postives = 326/327 (99.69%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAGEKAKTNPSSKPTNIRNEILQES+LASCTVNINKSEKSETQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESVLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           NRMVDSPSVP MQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP
Sbjct: 181 NRMVDSPSVPSMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKLDISDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of CsGy1G025350 vs. ExPASy TrEMBL
Match: A0A1S4DY73 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103492132 PE=4 SV=1)

HSP 1 Score: 585 bits (1509), Expect = 5.47e-210
Identity = 292/327 (89.30%), Postives = 308/327 (94.19%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC SIFRFPK+GVP+LANLWRENFHPLNRNHGH+CWYDQLCFRIYNLEGG+NKRWTRKSI
Sbjct: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKPTNI NEILQESM ASCTVN+NKSEKS TQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           EF H VTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLI+PQCYITNS+IHGI+
Sbjct: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           N MV S  VPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLL EFSRYS+DIP
Sbjct: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
            +WLF DTMTLARQLMKLSDSKITKI+L+AL EYYGI++DG+AHRAL DV  LS ILQRL
Sbjct: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKLD+SDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327

BLAST of CsGy1G025350 vs. ExPASy TrEMBL
Match: A0A6J1HUD7 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467897 PE=4 SV=1)

HSP 1 Score: 515 bits (1327), Expect = 3.46e-182
Identity = 261/327 (79.82%), Postives = 285/327 (87.16%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC SI R PK+GVP LANLWRENFHPLNRN+GH+ WYDQL FRIYNLEGG++KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKP+NIRNEIL+ES+  SCTV+INKSEK  TQKLQ C+IQ TIIK K
Sbjct: 66  TTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQETIIKDK 125

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           E A  VTVITFDIETTGFSR LDRIVEIA QDLSGGENSTFQTLINP+CYI N+ +HGI+
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           +RMV  P VPRM+ELIPI+LQFVKSRQKP GYVLLVAHNAR FDVPFLL EFSR SVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRCSVDIP 245

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFD + LARQLMK +DSKITKI+LQAL EYY I L G AHRA+SDVR LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327
           TFDLKL I DL+ER+FT  DLINKKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKK 332

BLAST of CsGy1G025350 vs. ExPASy TrEMBL
Match: A0A6J1HDU6 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461962 PE=4 SV=1)

HSP 1 Score: 511 bits (1315), Expect = 2.32e-180
Identity = 256/326 (78.53%), Postives = 285/326 (87.42%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC SI R PK+GVP LANLWRENFHPLNRN+GH+ WYDQL FR YNLEGG++KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120
           +TKAG KAKT+PSSKP+NIRNEIL+ES+  SCTV+INKSEK+ TQKLQ C+IQ TI+K K
Sbjct: 66  STKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQETIVKDK 125

Query: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180
           E A  VTVITFDIETTGFSR LDRIVEIA QDLSGGENSTFQTLINP+CYI N+ +HGI+
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240
           +RMV  P VPRM+ELIPI+LQF+KSRQKP GYVLLVAHNARTFDVPFLL EFSR SVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRCSVDIP 245

Query: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300
           LNWLFFD + LARQLMK +DSKITKI+LQAL EYY I L G AHRA+SDVR LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLDISDLVERAFTPLDLINKKK 326
           TFDLKL I DL+ER+FT  DLINKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of CsGy1G025350 vs. TAIR 10
Match: AT5G26940.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 5.7e-61
Identity = 138/326 (42.33%), Postives = 199/326 (61.04%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC+SI +  +  +    +   E  H   +N         L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKNSSS---LKLLDVRASSVDG--KARWIRRNV 60

Query: 61  -TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINK--SEKSETQKLQYCDIQPTII 120
            TT  G ++ T               +S +   TV + +   E+S T+   + ++Q  + 
Sbjct: 61  STTTQGSRSNT---------------KSSVLGGTVPVTRIIDEESRTKVQPFGNLQQRLA 120

Query: 121 KSKEFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCY-ITNSNI 180
           + K+ + L+TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    ITN++I
Sbjct: 121 QDKDLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHI 180

Query: 181 HGISNRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYS 240
           HGI N MV  P VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S
Sbjct: 181 HGIRNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCS 240

Query: 241 VDIPLNWLFFDTMTLARQLMKLSDSKI-TKITLQALGEYYGIKLDGKAHRALSDVRLLSS 300
            +IP NWL  D++ LAR+ MK  +  +    +L+AL +YY +  +G AHRALSDV LLS 
Sbjct: 241 YEIPHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSK 300

Query: 301 ILQRLTFDLKLDISDLVERAFTPLDL 322
           + Q+LT DLKL +SDLV R  T  D+
Sbjct: 301 VFQKLTIDLKLSLSDLVLRCHTASDI 306

BLAST of CsGy1G025350 vs. TAIR 10
Match: AT5G26940.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 5.7e-61
Identity = 138/326 (42.33%), Postives = 199/326 (61.04%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC+SI +  +  +    +   E  H   +N         L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKNSSS---LKLLDVRASSVDG--KARWIRRNV 60

Query: 61  -TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINK--SEKSETQKLQYCDIQPTII 120
            TT  G ++ T               +S +   TV + +   E+S T+   + ++Q  + 
Sbjct: 61  STTTQGSRSNT---------------KSSVLGGTVPVTRIIDEESRTKVQPFGNLQQRLA 120

Query: 121 KSKEFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCY-ITNSNI 180
           + K+ + L+TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    ITN++I
Sbjct: 121 QDKDLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHI 180

Query: 181 HGISNRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYS 240
           HGI N MV  P VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S
Sbjct: 181 HGIRNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCS 240

Query: 241 VDIPLNWLFFDTMTLARQLMKLSDSKI-TKITLQALGEYYGIKLDGKAHRALSDVRLLSS 300
            +IP NWL  D++ LAR+ MK  +  +    +L+AL +YY +  +G AHRALSDV LLS 
Sbjct: 241 YEIPHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSK 300

Query: 301 ILQRLTFDLKLDISDLVERAFTPLDL 322
           + Q+LT DLKL +SDLV R  T  D+
Sbjct: 301 VFQKLTIDLKLSLSDLVLRCHTASDI 306

BLAST of CsGy1G025350 vs. TAIR 10
Match: AT5G26940.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 5.7e-61
Identity = 138/326 (42.33%), Postives = 199/326 (61.04%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC+SI +  +  +    +   E  H   +N         L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKNSSS---LKLLDVRASSVDG--KARWIRRNV 60

Query: 61  -TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINK--SEKSETQKLQYCDIQPTII 120
            TT  G ++ T               +S +   TV + +   E+S T+   + ++Q  + 
Sbjct: 61  STTTQGSRSNT---------------KSSVLGGTVPVTRIIDEESRTKVQPFGNLQQRLA 120

Query: 121 KSKEFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCY-ITNSNI 180
           + K+ + L+TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    ITN++I
Sbjct: 121 QDKDLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHI 180

Query: 181 HGISNRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYS 240
           HGI N MV  P VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S
Sbjct: 181 HGIRNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCS 240

Query: 241 VDIPLNWLFFDTMTLARQLMKLSDSKI-TKITLQALGEYYGIKLDGKAHRALSDVRLLSS 300
            +IP NWL  D++ LAR+ MK  +  +    +L+AL +YY +  +G AHRALSDV LLS 
Sbjct: 241 YEIPHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSK 300

Query: 301 ILQRLTFDLKLDISDLVERAFTPLDL 322
           + Q+LT DLKL +SDLV R  T  D+
Sbjct: 301 VFQKLTIDLKLSLSDLVLRCHTASDI 306

BLAST of CsGy1G025350 vs. TAIR 10
Match: AT5G26940.4 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 5.7e-61
Identity = 138/326 (42.33%), Postives = 199/326 (61.04%), Query Frame = 0

Query: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60
           MC+SI +  +  +    +   E  H   +N         L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKNSSS---LKLLDVRASSVDG--KARWIRRNV 60

Query: 61  -TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINK--SEKSETQKLQYCDIQPTII 120
            TT  G ++ T               +S +   TV + +   E+S T+   + ++Q  + 
Sbjct: 61  STTTQGSRSNT---------------KSSVLGGTVPVTRIIDEESRTKVQPFGNLQQRLA 120

Query: 121 KSKEFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCY-ITNSNI 180
           + K+ + L+TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    ITN++I
Sbjct: 121 QDKDLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHI 180

Query: 181 HGISNRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYS 240
           HGI N MV  P VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S
Sbjct: 181 HGIRNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCS 240

Query: 241 VDIPLNWLFFDTMTLARQLMKLSDSKI-TKITLQALGEYYGIKLDGKAHRALSDVRLLSS 300
            +IP NWL  D++ LAR+ MK  +  +    +L+AL +YY +  +G AHRALSDV LLS 
Sbjct: 241 YEIPHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSK 300

Query: 301 ILQRLTFDLKLDISDLVERAFTPLDL 322
           + Q+LT DLKL +SDLV R  T  D+
Sbjct: 301 VFQKLTIDLKLSLSDLVLRCHTASDI 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q682U68.1e-6042.33Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8RA324.1e-1132.00DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongens... [more]
Q8XJR36.9e-1131.53DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=... [more]
Q895K23.4e-1030.00DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) ... [more]
Q8EQU64.2e-0832.04DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP... [more]
Match NameE-valueIdentityDescription
XP_004135590.14.85e-236100.00exonuclease DPD1, chloroplastic/mitochondrial [Cucumis sativus] >KGN66030.1 hypo... [more]
ALG62680.11.62e-23499.39exonuclease DPD1 [Cucumis sativus] >ALG62681.1 exonuclease DPD1 [Cucumis sativus... [more]
XP_008450585.11.13e-20989.30PREDICTED: exonuclease DPD1, chloroplastic/mitochondrial [Cucumis melo] >XP_0169... [more]
XP_022968752.17.14e-18279.82exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1... [more]
KAG6590475.18.31e-18178.83Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
A0A0A0LVY32.35e-236100.00Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080... [more]
A0A0N9LRH77.85e-23599.39Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1[more]
A0A1S4DY735.47e-21089.30exonuclease DPD1, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1HUD73.46e-18279.82exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HDU62.32e-18078.53exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G26940.15.7e-6142.33Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.25.7e-6142.33Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.35.7e-6142.33Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.45.7e-6142.33Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 127..306
e-value: 5.4E-30
score: 115.7
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 130..292
e-value: 9.7E-20
score: 71.7
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 126..310
e-value: 1.9E-43
score: 150.6
IPR040393Three-prime repair exonuclease 1/2PANTHERPTHR13058THREE PRIME REPAIR EXONUCLEASE 1, 2coord: 46..323
NoneNo IPR availablePANTHERPTHR13058:SF23POLYMERASE III POLC-TYPE, PUTATIVE ISOFORM 1-RELATEDcoord: 46..323
NoneNo IPR availableCDDcd06127DEDDhcoord: 131..298
e-value: 3.49938E-39
score: 133.965
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 127..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G025350.2CsGy1G025350.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000738 DNA catabolic process, exonucleolytic
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008408 3'-5' exonuclease activity