CsaV3_4G007800 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_4G007800
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	heme-binding protein 2
Location	chr4: 5451765 .. 5455292 (-)
RNA-Seq Expression	CsaV3_4G007800
Synteny	CsaV3_4G007800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTATTTTTAAAGAAAAGAAAATTAAGAATTTGAGTGAAATGAAATGTTGTGCAAGTTTGAAGAGCATATCTCCAAGTTTTTTTTTTTAGAAAAACGCGTGGACGTCCCTCATCCTCACTCACTGTGTGGCTTATCCTTTTTTTTATTATTAAGTTCTCTCATCGTTCTCTCTTCGACGAGTGTAATTTCCAATGGAAATGGCGAAGCTATGGGTTGTAGTGAGCTTGGTGATGATGATCTTGACGCCCGCATACTCGGAGTTTCCTCCGACCTGCAAGCGCATAGAGTGTCCTAGCTACGACGTCATCGGCACCGGCGATGGGTTCGAGATTCGGCGCTATAATTCCCCTGTTTGGGCCTCCACTGCTCCCATTCCCGATATCTCTCTCCGTGAAGCCACCAGAGCAGGCTTTCTCCAGTAACCTTCTACTTCTCTCCTTTTCCTATTTCTTACTTCTTATTTGAATCCATCCATCCTCTTAGGTTTTCTTTTCTCCATTTTCTCAAAACCCCTAATCTTTCATTCTGCATATTTTTCTTCCTTTTTCTGTTATTCTCTTCCTAATCCAATTTAGCCCTTTGTGACCATCTCTTTTCTTCTATCAAATTCAAATGTCAATTTTTCTTTTTCTTTTTCTTTTTTTTTTTTTAAAAAAATTGTTTTGATTTCATTAAATTTTAATACTAATTTTCACAATCAATATAAAATCACTCTCAAATCTAAAATGTAAACATTAAATTATTATATATAATATACCGAAAACTGAAGCTTTTTGGTTCAAAACTCCGTAGTTGAATTGTACTTTAAAGAAACCTATTTAAATAATCTCAATTACACTATTGACTTTAAAAGTCACCATTTCTTTTACAAATACTCTAATGGACTGGAATTTTCTCCTTCAAATCATTTAATATTTGAAAAAAAATCAATAAAAAACATTAATGGAAACTGTGAATGAATTCTTACTTTAAAAAAAAAATAGCGTAATAATAGTTTTCCATAACCGTCTCTCTCAAGTCCCCACTCATCCCTCAGGCCTCAATAATTATAGTCTTCGTAAGAATTCTTTTACACTTATTTCTTTTATTTATGTTTATTGGGTTTTTTCTATTTTCTATATGTAGTTGTCCTATGGACGTTTAAATTAACATTTTATGTTACAATTTATCTTCTAGCATATCACATATATAATGTTATTTATGTTGTATTTATCTTCTGAAACTTTATTTATTGAAACTAATTTATACTATATATATCTCATATATAATGTCCAAATTAAATATATATATATATATATGCAATAAGTAATCGGATATCTGTTGGATTCTAAAAAATTATGATTTATTGAAAGTCACTATGAGCTATGGTACTTAGTATACATGGGGTTTATTTCTATACTTGTTTTCAATTTTAAATGTATATTGTTTTATTTCAAATAATATGAGATGTCTTCCATATTAGTTTTATATACATGTGATTCTTTCCTAGATAATCTGAGTATATACAGTGATTAGTTTATATTCATATAAATGAATATATTTATAATCTGAACATAATTACCTTAACAAGTTCCTAAAATACAGTGGTTTTTTCCTTGACAGTTAGAGAATACAAGAAGAAAGAAAAAACATTAAATTAAAACCTCACTGTTAATTACAATTCACATTGTATTTAATTCTACAAAAATAAAATAGCACTGTTTTGGTAAAATCAACGACAATATTTTAATGTTAAATCATGGTAGAAAAGAGTATCAATTAATTAAAGAGAGAGTGCTACAGAGTTAAATTTGTCTCAAATTCTTTTTTGTAGAAACCTATATATGTAGATTAATTCAAGACTATGAAATATTGCCTTTGAAGTAGGGTATTGTAGTGAATTGTAGTGAATTGTAGTGAATTAAAGAAACATTTTGAATATTAATATTTAGGAGTGAATTGTAGTCTCTCATTTCAGTATGGAGTTGATTTAGATGATGGGTTTGGTCCTCCCTATCTCCTCTTTTGGTAATGGTAATGGTAATGGAACTCTAAGCTTTGGGGTTGGGCTTTTCTCTTTAGTTCAAAATTTTCCCTCTACCTATGTTAAATAAATAAAAGTTAGTATAAGTCTCAAGTATAACATATAACTCCTCTAAGAAACTAGAGAAACCTTAAGGAGGAGTAGGTATGTAGTAGTTCCTATCAACTTTCCTGAGTATAAACTCATCTAGTTCGATTAAGACACTATACCATCCATCTAAAAGTTGGGTTAAAAGTTTAGCTAAAAAGTTGGGTTAAAAGTTTAGCTAAAAAGTTGACTTTTAAAGTTGAATGGTTTCAAGCAAAATATTTTGTTAAATTAATAGTTCTACTGTTATGAGTTTGTGTTCATTTTGAACCTTAAAATGTGTGTAGTAAGTTATTAAACTTTTAATCTTATGTCTAATTTAGAAAAGTAAAGTAGATTCAAACTTATAAGTTCATCTAACTTATAAAAGACCACAATATTACAGTACTTCAACCAAGAAAGTATCTTTTTATTCCCAATGATAAAGATATTTTATGAATCAAATTTGTCCTTTGAAGATTAAAGGACTCTCTTAAAGGTGTCAGTCATTGTAGACAATTCACGTCTACTAAGGAGTTTGATGATTACTTAAACATCTTTTCTTTTTTTTTTCTTTTTTTTTTTTGAATTTTCGATCACTTTTAGACCTTTCATCAGTATTGCCTTGAATTTTTGGCACGGCTACTTAGCTAATTATTGGTTCTTACTCGGCATTGAGACTTATTAGACACCAAACTTGTGGTTCAACCCTACATTTTTTCACCTTGAAGTTGTTGGTTTGCATCAAATAATTTCTTGAACTTTTAATTTGATCTTGATTTGATTCAGTAGATTGACTTGGTTTGCTTCTTTCATTAGATTGTTTGATTACATTCAAGGAAAGAACAGTTTCAACGAAAAGATAGAAATGACGGGACCGGTGATCACAGAGATTTCTCCGAGCGACGGACCTTTTTGTGAATCATCATTTGTTGTCAGCTTCTACGTTCCGAAAATCAACCAAGCAGATCCACCACCAGCCAAAGGGCTTCATATACAGAGATGGAACTCAACATATGTAGCAGTTAGACAATTTGGAGGATTTGTTACTGATGCAAACATTGGAAGTGAAGCTTCTGCTTTGGATGAAAGTGTTTTTGATACAAAGTGGGGTGCAGCAATCAGCAAGAGCCGTGGCGCTGCCGGTCCTTCCATTTATACTGTAGCTCAGTACAATTCTCCTTTTGAGTTTGAAGGTCGAGTCAATGAGATTTGGTTCTTGTTTGATATTGAAGATGTAGCTGCTCAGGCTTAGAATATAATTTTAGTGTTTTATGCTAAATCAAATCAAAAGACTCGTTCTCTTGATCTTTTATGATCCAATCCTTCAATAAAATATATTCACTTTCCTCTGTCTTTGGAAGTTGTACACATTGTTCTTTTGTTTCGTTTGTTCAAATGTTGAGTTTTAGTAATGCCTATGCATTTCTATTCCTAC

mRNA sequence

ATGGAAATGGCGAAGCTATGGGTTGTAGTGAGCTTGGTGATGATGATCTTGACGCCCGCATACTCGGAGTTTCCTCCGACCTGCAAGCGCATAGAGTGTCCTAGCTACGACGTCATCGGCACCGGCGATGGGTTCGAGATTCGGCGCTATAATTCCCCTGTTTGGGCCTCCACTGCTCCCATTCCCGATATCTCTCTCCGTGAAGCCACCAGAGCAGGCTTTCTCCAATTGTTTGATTACATTCAAGGAAAGAACAGTTTCAACGAAAAGATAGAAATGACGGGACCGGTGATCACAGAGATTTCTCCGAGCGACGGACCTTTTTGTGAATCATCATTTGTTGTCAGCTTCTACGTTCCGAAAATCAACCAAGCAGATCCACCACCAGCCAAAGGGCTTCATATACAGAGATGGAACTCAACATATGTAGCAGTTAGACAATTTGGAGGATTTGTTACTGATGCAAACATTGGAAGTGAAGCTTCTGCTTTGGATGAAAGTGTTTTTGATACAAAGTGGGGTGCAGCAATCAGCAAGAGCCGTGGCGCTGCCGGTCCTTCCATTTATACTGTAGCTCAGTACAATTCTCCTTTTGAGTTTGAAGGTCGAGTCAATGAGATTTGGTTCTTGTTTGATATTGAAGATGTAGCTGCTCAGGCTTAG

Coding sequence (CDS)

ATGGAAATGGCGAAGCTATGGGTTGTAGTGAGCTTGGTGATGATGATCTTGACGCCCGCATACTCGGAGTTTCCTCCGACCTGCAAGCGCATAGAGTGTCCTAGCTACGACGTCATCGGCACCGGCGATGGGTTCGAGATTCGGCGCTATAATTCCCCTGTTTGGGCCTCCACTGCTCCCATTCCCGATATCTCTCTCCGTGAAGCCACCAGAGCAGGCTTTCTCCAATTGTTTGATTACATTCAAGGAAAGAACAGTTTCAACGAAAAGATAGAAATGACGGGACCGGTGATCACAGAGATTTCTCCGAGCGACGGACCTTTTTGTGAATCATCATTTGTTGTCAGCTTCTACGTTCCGAAAATCAACCAAGCAGATCCACCACCAGCCAAAGGGCTTCATATACAGAGATGGAACTCAACATATGTAGCAGTTAGACAATTTGGAGGATTTGTTACTGATGCAAACATTGGAAGTGAAGCTTCTGCTTTGGATGAAAGTGTTTTTGATACAAAGTGGGGTGCAGCAATCAGCAAGAGCCGTGGCGCTGCCGGTCCTTCCATTTATACTGTAGCTCAGTACAATTCTCCTTTTGAGTTTGAAGGTCGAGTCAATGAGATTTGGTTCTTGTTTGATATTGAAGATGTAGCTGCTCAGGCTTAG

Protein sequence

MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAPIPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSRGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA*

Homology

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BLAST of CsaV3_4G007800 vs. NCBI nr
Match: XP_004137252.1 (heme-binding protein 2 [Cucumis sativus] >KGN53540.1 hypothetical protein Csa_015165 [Cucumis sativus])

HSP 1 Score: 455.7 bits (1171), Expect = 2.3e-124
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60
           MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP
Sbjct: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60

Query: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120
           IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120

Query: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180
           KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180

Query: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA
Sbjct: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 220

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BLAST of CsaV3_4G007800 vs. NCBI nr
Match: XP_008451794.1 (PREDICTED: heme-binding protein 2 [Cucumis melo] >KAA0063003.1 heme-binding protein 2 [Cucumis melo var. makuwa] >TYK16344.1 heme-binding protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 435.6 bits (1119), Expect = 2.5e-118
Identity = 209/220 (95.00%), Postives = 215/220 (97.73%), Query Frame = 0


Query: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60
           MEM +LW VVSLVM+ILTPAYSEFPPTCKRIECPSYDVI TGDGFEIRRYNSPVWASTAP
Sbjct: 1   MEMGRLW-VVSLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120
           IPDISL EATRAGFLQLFDYIQGKN +NEKIEMTGPVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180
           KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEA+ALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           RGAAGPS+YTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 219

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BLAST of CsaV3_4G007800 vs. NCBI nr
Match: XP_038903399.1 (heme-binding protein 2-like [Benincasa hispida])

HSP 1 Score: 403.7 bits (1036), Expect = 1.0e-108
Identity = 192/217 (88.48%), Postives = 205/217 (94.47%), Query Frame = 0


Query: 3   MAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAPIP 62
           M KLW V+SLVM+IL PAYS+FPPTC RIECP+YDVI +GD FEIRRYNSPVWASTAPIP
Sbjct: 1   MVKLWGVLSLVMVILMPAYSKFPPTCNRIECPAYDVIDSGDEFEIRRYNSPVWASTAPIP 60

Query: 63  DISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKI 122
           DISLREATRAGFLQLFDYIQGKNS+NEKIEMT PVITE+SPSDGPFCESSFVVSFYVP +
Sbjct: 61  DISLREATRAGFLQLFDYIQGKNSYNEKIEMTAPVITEVSPSDGPFCESSFVVSFYVPGV 120

Query: 123 NQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSR- 182
           NQADPPPA+GLH+QRWNSTYVAVRQFGGFV+D NIGSEA+ALDESVFDTKWGAAISKSR 
Sbjct: 121 NQADPPPARGLHVQRWNSTYVAVRQFGGFVSDENIGSEAAALDESVFDTKWGAAISKSRG 180

Query: 183 GAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAA 219
           G +G SIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAA
Sbjct: 181 GGSGASIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAA 217

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BLAST of CsaV3_4G007800 vs. NCBI nr
Match: XP_023552168.1 (heme-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.4 bits (999), Expect = 2.0e-104
Identity = 181/218 (83.03%), Postives = 200/218 (91.74%), Query Frame = 0


Query: 3   MAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAPIP 62
           + KL ++ +L+++IL PAYSE PP+CKRIECPSYDVI +G GFEIRRY+SPVWASTAPIP
Sbjct: 6   IVKLSLLATLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSPVWASTAPIP 65

Query: 63  DISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKI 122
           DISL EATR GFL+LFDYIQGKN +NEKIEMT PVITE+SPSDGPFCESSFVVSFYVPK+
Sbjct: 66  DISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVITEVSPSDGPFCESSFVVSFYVPKV 125

Query: 123 NQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSRG 182
           NQADPPPAKGLHIQRW  TYVAVRQFGGFV+DA IG+EA+ALDESV+DTKWGAAISKSRG
Sbjct: 126 NQADPPPAKGLHIQRWKETYVAVRQFGGFVSDATIGAEAAALDESVYDTKWGAAISKSRG 185

Query: 183 AAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           A G +IYTVAQYNSPFEFEGRVNEIWFLFDIED AA A
Sbjct: 186 AGGAAIYTVAQYNSPFEFEGRVNEIWFLFDIEDAAAAA 223

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BLAST of CsaV3_4G007800 vs. NCBI nr
Match: KAG7015067.1 (Heme-binding protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 386.7 bits (992), Expect = 1.3e-103
Identity = 179/218 (82.11%), Postives = 199/218 (91.28%), Query Frame = 0


Query: 3   MAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAPIP 62
           + KL ++ +L+++IL PAYSE PP+CKRIECPSYDVI +G GFEIRRY+SPVWASTAPIP
Sbjct: 6   IVKLSLLATLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSPVWASTAPIP 65

Query: 63  DISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKI 122
           DISL EATR GFL+LFDYIQGKN +NEKIEMT PV+TE+SPSDGPFCESSFVVSFYVPK+
Sbjct: 66  DISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVPKV 125

Query: 123 NQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSRG 182
           NQADPPPAKGLHIQRW  TYVAVRQFGGFV+DA IG+EA+ALDESV+DTKWGAAISKSRG
Sbjct: 126 NQADPPPAKGLHIQRWKETYVAVRQFGGFVSDATIGAEAAALDESVYDTKWGAAISKSRG 185

Query: 183 AAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           A G + YTVAQYNSPFEFEGRVNEIWFLFDIED AA A
Sbjct: 186 AGGAATYTVAQYNSPFEFEGRVNEIWFLFDIEDAAAAA 223

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BLAST of CsaV3_4G007800 vs. ExPASy Swiss-Prot
Match: Q9Y5Z4 (Heme-binding protein 2 OS=Homo sapiens OX=9606 GN=HEBP2 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 61/174 (35.06%), Postives = 82/174 (47.13%), Query Frame = 0


Query: 45  FEIRRYNSPVWASTAPIPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPS 104
           +EIR Y    W ST+ +  +    A + GF +L  YIQGKN    KI+MT PV + + P 
Sbjct: 38  YEIRHYGPAKWVSTS-VESMDWDSAIQTGFTKLNSYIQGKNEKEMKIKMTAPVTSYVEPG 97

Query: 105 DGPFCESSFVVSFYVPKINQADPPP--AKGLHIQRWNSTYVAVRQFGGFVT----DANIG 164
            GPF ES+  +S Y+P   Q DPP      + I+      V VR F GF +       + 
Sbjct: 98  SGPFSESTITISLYIPSEQQFDPPRPLESDVFIEDRAEMTVFVRSFDGFSSAQKNQEQLL 157

Query: 165 SEASAL--DESVFDTKWGAAISKSRGAAGPSIYTVAQYNSPFEFEGRVNEIWFL 211
           + AS L  D  VFD K               +Y  A YNSP +   R NE+W +
Sbjct: 158 TLASILREDGKVFDEK---------------VYYTAGYNSPVKLLNRNNEVWLI 195

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BLAST of CsaV3_4G007800 vs. ExPASy Swiss-Prot
Match: Q9WU63 (Heme-binding protein 2 OS=Mus musculus OX=10090 GN=Hebp2 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.8e-18
Identity = 59/187 (31.55%), Postives = 87/187 (46.52%), Query Frame = 0


Query: 31  IECPSY----DVIGTGDGFEIRRYNSPVWASTAPIPDISLREATRAGFLQLFDYIQGKNS 90
           +E PS+    D+      +EIR Y    W ST  +  +    A + GF +L  YIQGKN 
Sbjct: 20  LEMPSWKAPEDIDPQPGSYEIRHYGPAKWVSTC-VESLDWDSAIQTGFTKLNGYIQGKNE 79

Query: 91  FNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKINQADPP-PAKG-LHIQRWNSTYVA 150
              KI++T PV + + P   PF ES+  +S Y+P   Q DPP P++  + I+      V 
Sbjct: 80  KEMKIKLTAPVTSYVEPGSSPFSESTITISLYIPSEQQPDPPRPSESDVFIEDRAEMTVF 139

Query: 151 VRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSRGAA-GPSIYTVAQYNSPFEFEGR 210
           VR F GF +      +   L          A I +  G      ++  A Y+SPF+   R
Sbjct: 140 VRSFDGFSSGQKNQEQLLTL----------ANILREEGKVFNEKVFYTAGYSSPFQLLDR 195

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BLAST of CsaV3_4G007800 vs. ExPASy Swiss-Prot
Match: Q9SR77 (Heme-binding-like protein At3g10130, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g10130 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.4e-07
Identity = 55/185 (29.73%), Postives = 85/185 (45.95%), Query Frame = 0


Query: 31  IECPSYDVIGTGDGFEIRRYNSPVWASTAPIPDISLREATRA--GFLQLFDYIQGKNSFN 90
           +E  ++ V+   D +EIR+   P + +   +P  +  ++  A   F  L +Y+ GKN+  
Sbjct: 114 LETMNFRVLFRTDKYEIRQV-EPYFVAETIMPGETGFDSYGASKSFNVLAEYLFGKNTIK 173

Query: 91  EKIEMTGPVITEISPSDGPFCE-------------SSFVVSFYVPKINQADPPPAK--GL 150
           EK+EMT PV+T    S G   E             + + +SF +P    ++ P  K   +
Sbjct: 174 EKMEMTTPVVTRKVQSVGEKMEMTTPVITSKAKDQNQWRMSFVMPSKYGSNLPLPKDPSV 233

Query: 151 HIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSRGAAGPSIYTVAQ 199
            IQ+     VAV  F G+VTD  I      L  ++ + K      K R   G S + VAQ
Sbjct: 234 KIQQVPRKIVAVVAFSGYVTDEEIERRERELRRALQNDK------KFRVRDGVS-FEVAQ 290

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BLAST of CsaV3_4G007800 vs. ExPASy TrEMBL
Match: A0A0A0KVD6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075210 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.1e-124
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60
           MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP
Sbjct: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60

Query: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120
           IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120

Query: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180
           KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180

Query: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA
Sbjct: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 220

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BLAST of CsaV3_4G007800 vs. ExPASy TrEMBL
Match: A0A5A7V4M9 (Heme-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001260 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 209/220 (95.00%), Postives = 215/220 (97.73%), Query Frame = 0


Query: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60
           MEM +LW VVSLVM+ILTPAYSEFPPTCKRIECPSYDVI TGDGFEIRRYNSPVWASTAP
Sbjct: 1   MEMGRLW-VVSLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120
           IPDISL EATRAGFLQLFDYIQGKN +NEKIEMTGPVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180
           KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEA+ALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           RGAAGPS+YTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 219

[Close]

BLAST of CsaV3_4G007800 vs. ExPASy TrEMBL
Match: A0A1S3BSD4 (heme-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103492972 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 209/220 (95.00%), Postives = 215/220 (97.73%), Query Frame = 0


Query: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60
           MEM +LW VVSLVM+ILTPAYSEFPPTCKRIECPSYDVI TGDGFEIRRYNSPVWASTAP
Sbjct: 1   MEMGRLW-VVSLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120
           IPDISL EATRAGFLQLFDYIQGKN +NEKIEMTGPVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180
           KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEA+ALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           RGAAGPS+YTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 219

[Close]

BLAST of CsaV3_4G007800 vs. ExPASy TrEMBL
Match: A0A6J1EZ63 (heme-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437561 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 6.4e-104
Identity = 179/218 (82.11%), Postives = 199/218 (91.28%), Query Frame = 0


Query: 3   MAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAPIP 62
           + KL ++ +L+++IL PAYSE PP+CKRIECPSYDVI +G GFEIRRY+SPVWASTAPIP
Sbjct: 6   IVKLSLLATLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSPVWASTAPIP 65

Query: 63  DISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKI 122
           DISL EATR GFL+LFDYIQGKN +NEKIEMT PV+TE+SPSDGPFCESSFVVSFYVPK+
Sbjct: 66  DISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVPKV 125

Query: 123 NQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSRG 182
           NQADPPPAKGLHIQRW  TYVAVRQFGGFV+DA IG+EA+ALDESV+DTKWGAAISKSRG
Sbjct: 126 NQADPPPAKGLHIQRWKETYVAVRQFGGFVSDATIGAEAAALDESVYDTKWGAAISKSRG 185

Query: 183 AAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           A G + YTVAQYNSPFEFEGRVNEIWFLFDIED AA A
Sbjct: 186 AGGAATYTVAQYNSPFEFEGRVNEIWFLFDIEDAAAAA 223

[Close]

BLAST of CsaV3_4G007800 vs. ExPASy TrEMBL
Match: A0A6J1J8G7 (heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482697 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.1e-103
Identity = 180/220 (81.82%), Postives = 200/220 (90.91%), Query Frame = 0


Query: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60
           +EM KL ++ +L+++IL PAYSE PP+CKRIECPSYDVI +G GFEIRRY+S VWASTAP
Sbjct: 6   IEMLKLSLLTTLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSSVWASTAP 65

Query: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120
           IPDISL EATR GFL+LFDYIQGKN +NEKIEMT PV+TE+SPSDGPFCESSFVVSFYVP
Sbjct: 66  IPDISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVP 125

Query: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180
           K+NQADPPPAKGLHIQRW  T+VAVRQFGGFV+DA IG+EA+ALDESVFDTKW AAISKS
Sbjct: 126 KVNQADPPPAKGLHIQRWKETHVAVRQFGGFVSDATIGAEAAALDESVFDTKWAAAISKS 185

Query: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 221
           RGA G +IYTVAQYNSPFEFEGRVNEIWFLFDIED AA A
Sbjct: 186 RGAGGAAIYTVAQYNSPFEFEGRVNEIWFLFDIEDAAADA 225

[Close]

BLAST of CsaV3_4G007800 vs. TAIR 10
Match: AT1G17100.1 (SOUL heme-binding family protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.1e-76
Identity = 128/195 (65.64%), Postives = 157/195 (80.51%), Query Frame = 0


Query: 23  EFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAPIPDISLREATRAGFLQLFDYIQ 82
           +FPP+C RIECPSY+++ +G+G+EIRRYN+ VW ST PIPDISL +ATR  F QLF YIQ
Sbjct: 37  KFPPSCNRIECPSYELVHSGNGYEIRRYNNTVWVSTEPIPDISLVDATRTAFFQLFAYIQ 96

Query: 83  GKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKINQADPPPAKGLHIQRWNSTY 142
           GKN +++KIEMT PVI+++SPSDGPFCESSF VSFYVPK NQ DP P++ LHIQ+WNS Y
Sbjct: 97  GKNEYHQKIEMTAPVISQVSPSDGPFCESSFTVSFYVPKKNQPDPAPSENLHIQKWNSRY 156

Query: 143 VAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSR---GAAGPSIYTVAQYNSPFE 202
           VAVRQF GFV+D +IG +A+ALD S+  T W  AI+KS+   G    S YTVAQYNSPFE
Sbjct: 157 VAVRQFSGFVSDDSIGEQAAALDSSLKGTAWANAIAKSKEDGGVGSDSAYTVAQYNSPFE 216

Query: 203 FEGRVNEIWFLFDIE 215
           F GRVNEIW  F+++
Sbjct: 217 FSGRVNEIWLPFELD 231

[Close]

BLAST of CsaV3_4G007800 vs. TAIR 10
Match: AT1G78450.1 (SOUL heme-binding family protein )

HSP 1 Score: 208.4 bits (529), Expect = 6.0e-54
Identity = 98/194 (50.52%), Postives = 130/194 (67.01%), Query Frame = 0


Query: 25  PPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAPIPDISLREATRAGFLQLFDYIQGK 84
           PP C R ECPSY+V+  G+G+EI RYN+ VW ST PI DISL EA+  G+ QL DY+ G 
Sbjct: 28  PPNCNRAECPSYEVVHAGNGYEIHRYNTTVWISTEPIQDISLNEASGNGWNQLSDYMNGN 87

Query: 85  NSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVPKINQADPPPAKGLHIQRWNSTYVA 144
           N ++++IE+  P IT++S +      S+F+VSF+VPK  Q DPPP   LH+QRW+S YVA
Sbjct: 88  NDYHQRIEIALPYITQVSQN-----LSTFIVSFFVPKAFQPDPPPGNNLHVQRWDSRYVA 147

Query: 145 VRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSR---GAAGPSIYTVAQYNSPFEFE 204
           V+Q  G+V D  IG + + L  S+  T W  AI KSR   G      YTVAQ++ PF++ 
Sbjct: 148 VKQISGYVADHKIGKQVAELKASLQGTVWAKAIEKSRETGGVGSAWAYTVAQFSWPFQWS 207

Query: 205 GRVNEIWFLFDIED 216
            RVNEIWF F++ED
Sbjct: 208 QRVNEIWFPFEMED 216

[Close]

BLAST of CsaV3_4G007800 vs. TAIR 10
Match: AT1G78460.1 (SOUL heme-binding family protein )

HSP 1 Score: 160.2 bits (404), Expect = 1.9e-39
Identity = 91/222 (40.99%), Postives = 128/222 (57.66%), Query Frame = 0


Query: 1   MEMAKLWVVVSLVMM--------ILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNS 60
           + + KL + VSLV++           P+    P TC   ECP+Y ++  G GFEIR Y++
Sbjct: 5   LNILKLSLCVSLVVVGSYAQAPAPWNPSNGFRPGTCDHYECPTYKLVEAGYGFEIRMYDA 64

Query: 61  PVWASTAPIPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESS 120
            +W ST+PIP +S+ +AT+ GF +L  YI+G N  N K+ MT PVI + +P       S 
Sbjct: 65  ALWISTSPIPSLSMTQATKTGFRRLNRYIEGDNKSNVKMNMTAPVIAQATPG-----RSV 124

Query: 121 FVVSFYVPKINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTK 180
           + VS Y+PK NQ +PP A  LH++    TYVAVRQ GG+V++     EA+AL ES+ D+ 
Sbjct: 125 YTVSLYLPKKNQQNPPQADDLHVRSTKPTYVAVRQIGGYVSNNVAKDEAAALMESLRDSN 184

Query: 181 WGAAISKSRGAAGPSIYTVAQYNSPFEFEGRV-NEIWFLFDI 214
           W   I KS+G      Y +A YN P     RV NEI   F++
Sbjct: 185 WILPIEKSKGKL--PAYFLAVYNPPSHTTARVINEIMVPFNM 219

[Close]

BLAST of CsaV3_4G007800 vs. TAIR 10
Match: AT3G10130.1 (SOUL heme-binding family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.0e-08
Identity = 55/185 (29.73%), Postives = 85/185 (45.95%), Query Frame = 0


Query: 31  IECPSYDVIGTGDGFEIRRYNSPVWASTAPIPDISLREATRA--GFLQLFDYIQGKNSFN 90
           +E  ++ V+   D +EIR+   P + +   +P  +  ++  A   F  L +Y+ GKN+  
Sbjct: 114 LETMNFRVLFRTDKYEIRQV-EPYFVAETIMPGETGFDSYGASKSFNVLAEYLFGKNTIK 173

Query: 91  EKIEMTGPVITEISPSDGPFCE-------------SSFVVSFYVPKINQADPPPAK--GL 150
           EK+EMT PV+T    S G   E             + + +SF +P    ++ P  K   +
Sbjct: 174 EKMEMTTPVVTRKVQSVGEKMEMTTPVITSKAKDQNQWRMSFVMPSKYGSNLPLPKDPSV 233

Query: 151 HIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKSRGAAGPSIYTVAQ 199
            IQ+     VAV  F G+VTD  I      L  ++ + K      K R   G S + VAQ
Sbjct: 234 KIQQVPRKIVAVVAFSGYVTDEEIERRERELRRALQNDK------KFRVRDGVS-FEVAQ 290

[Close]

BLAST of CsaV3_4G007800 vs. TAIR 10
Match: AT5G20140.1 (SOUL heme-binding family protein )

HSP 1 Score: 51.6 bits (122), Expect = 9.4e-07
Identity = 41/170 (24.12%), Postives = 74/170 (43.53%), Query Frame = 0


Query: 31  IECPSYDVIGTGDGFEIRRYNSPVWASTAPIPDISLREATRAGFLQLFDYIQGKNSFNEK 90
           +E P Y ++     +E+R Y   +   T     I  + +  +GF  +  YI GKNS  EK
Sbjct: 205 LETPKYQILKRTANYEVRNYEPFIVVET-----IGDKLSGSSGFNNVAGYIFGKNSTMEK 264

Query: 91  IEMTGPVITEISPSDGPFCESSFVVSFYVPK---INQADPPPAKGLHIQRWNSTYVAVRQ 150
           I MT PV T+ + +      S   V   +P    ++    P  + +++++    + A  +
Sbjct: 265 IPMTTPVFTQTTDTQ---LSSDVSVQIVIPSGKDLSSLPMPNEEKVNLKKLEGGFAAAVK 324

Query: 151 FGGFVTDANIGSEASALDESVFDTKWGAAISKSRGAAGPSIYTVAQYNSP 198
           F G  T+  + ++ + L  S+  +K G    K           +A+YN P
Sbjct: 325 FSGKPTEDVVQAKENELRSSL--SKDGLRAKKG--------CMLARYNDP 356

The following BLAST results are available for this feature:

BLAST of CsaV3_4G007800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137252.1	2.3e-124	100.00	heme-binding protein 2 [Cucumis sativus] >KGN53540.1 hypothetical protein Csa_01...	[more]
XP_008451794.1	2.5e-118	95.00	PREDICTED: heme-binding protein 2 [Cucumis melo] >KAA0063003.1 heme-binding prot...	[more]
XP_038903399.1	1.0e-108	88.48	heme-binding protein 2-like [Benincasa hispida]	[more]
XP_023552168.1	2.0e-104	83.03	heme-binding protein 2-like [Cucurbita pepo subsp. pepo]	[more]
KAG7015067.1	1.3e-103	82.11	Heme-binding protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CsaV3_4G007800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y5Z4	1.6e-19	35.06	Heme-binding protein 2 OS=Homo sapiens OX=9606 GN=HEBP2 PE=1 SV=1	[more]
Q9WU63	1.8e-18	31.55	Heme-binding protein 2 OS=Mus musculus OX=10090 GN=Hebp2 PE=1 SV=1	[more]
Q9SR77	1.4e-07	29.73	Heme-binding-like protein At3g10130, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]

BLAST of CsaV3_4G007800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KVD6	1.1e-124	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075210 PE=3 SV=1	[more]
A0A5A7V4M9	1.2e-118	95.00	Heme-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A1S3BSD4	1.2e-118	95.00	heme-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103492972 PE=3 SV=1	[more]
A0A6J1EZ63	6.4e-104	82.11	heme-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437561 PE=3 S...	[more]
A0A6J1J8G7	1.1e-103	81.82	heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482697 PE=3 SV=...	[more]

BLAST of CsaV3_4G007800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17100.1	1.1e-76	65.64	SOUL heme-binding family protein	[more]
AT1G78450.1	6.0e-54	50.52	SOUL heme-binding family protein	[more]
AT1G78460.1	1.9e-39	40.99	SOUL heme-binding family protein	[more]
AT3G10130.1	1.0e-08	29.73	SOUL heme-binding family protein	[more]
AT5G20140.1	9.4e-07	24.12	SOUL heme-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006917	SOUL haem-binding protein	PFAM	PF04832	SOUL	coord: 31..209 e-value: 2.5E-44 score: 151.4
IPR006917	SOUL haem-binding protein	PANTHER	PTHR11220	HEME-BINDING PROTEIN-RELATED	coord: 11..217
IPR011256	Regulatory factor, effector binding domain superfamily	GENE3D	3.20.80.10	Regulatory factor, effector binding domain	coord: 30..218 e-value: 2.2E-50 score: 172.9
IPR011256	Regulatory factor, effector binding domain superfamily	SUPERFAMILY	55136	Probable bacterial effector-binding domain	coord: 24..211
None	No IPR available	PANTHER	PTHR11220:SF25	F3F9.4	coord: 11..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_4G007800.1	CsaV3_4G007800.1	mRNA