Tan0022681.1 (mRNA) Snake gourd v1

BLAST of Tan0022681.1 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 8.3e-289
Identity = 497/777 (63.96%), Postives = 596/777 (76.71%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MA   L S  F F +L       S S +  E++IVHV +S KPSLFSSH++WH S+L SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
             SP P  LLY+Y RA +GFS R++  Q A LR+ P VISVIPD+  ++HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           + + GLW  +NY +DVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  FPAS+CN
Sbjct: 121 SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCN 180

Query: 181 RKIIGARAYFYGY-ESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           RK+IGARA++ GY     G   H + + +S RDT+GHGTHTASTAAGS V NAS +QYAR
Sbjct: 181 RKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           G A GMAS+ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DS
Sbjct: 241 GTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVF 360
           IAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG VF
Sbjct: 301 IAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           +G SLY+G+ L DS+L LVY+GDCG+R CY G L+SS V GKIV+CDRGGNARV KGSAV
Sbjct: 361 TGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAV 420

Query: 421 KAAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTV 480
           K AGG G+IL NT E+GEEL ADSHL+P TM+G  AG++++DYI +  SPTA I F GT+
Sbjct: 421 KLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTL 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG SPP+PRVAAFSSRGPN+ TP ILKPDVIAPGVNILAGW+G   PT L IDPRRV+FN
Sbjct: 481 IGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNP 600
           IISGTSMSCPHVSG+AALLRKA P WSPAAIKSALVTT+Y +++SG PI+DL+T K SN 
Sbjct: 541 IISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNS 600

Query: 601 FVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCE-HKLS 660
           F+HGAGH+DPNKALNPGL+YDI  ++YV+FLC++GY+   I VF++D +    CE  KL 
Sbjct: 601 FIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLR 660

Query: 661 SPGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEF 720
           + G+LNYPSF+VVF S G EVVKY R V NVG   +AVYEV V++P  VEI V P+KL F
Sbjct: 661 TAGDLNYPSFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAF 720

Query: 721 SAKNPTQSYEITFTKI-----NGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISS 771
           S +     YE+TF  +      G      FGSI+W+DG H+V+SP+AV    G + S
Sbjct: 721 SKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Tan0022681.1 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 1.0e-230
Identity = 427/764 (55.89%), Postives = 536/764 (70.16%), Query Frame = 0

Query: 8   SSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPT 67
           SS  FF++LC      S S  DQ T+IVH++KS+ PS F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  RLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGLAD-SLGL 127
            LLY YE A +GFSTR+T  +A  L   PGVISV+P+  ++LHTTRTP FLGL + +  L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACNRKIIGA 187
           +PE     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CE G  F AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMA 247
           R +  GYES +G  + +S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA
Sbjct: 187 RFFARGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 248 SRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 307
            RAR+A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 308 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFSGVSLYS 367
           AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G  F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 368 GDPLGDSKLPLVYAGDCGN----RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKAA 427
           G+ L D  LP +YAG+  N      C +GTL   KV GKIV+CDRG NARV KG  VKAA
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 428 GGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVIGD 487
           GG+G+IL NT  NGEEL+AD+HL+P T +GE AG+ ++ Y+ +DP+PTA+I   GTV+G 
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 488 SPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIIS 547
             P+P VAAFSSRGPN  TP ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 548 GTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPFVH 607
           GTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 608 GAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCE-HKLSSPG 667
           GAGH+ P  A NPGLIYD+  +DY+ FLC++ Y S QI   V   +Y   C+  K  S  
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR-SVSRRNYT--CDPSKSYSVA 666

Query: 668 NLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEA-PQGVEISVLPNKLEFSA 727
           +LNYPSFAV  + DG    KYTRTVT+VG      Y VKV +   GV+ISV P  L F  
Sbjct: 667 DLNYPSFAV--NVDGVGAYKYTRTVTSVG--GAGTYSVKVTSETTGVKISVEPAVLNFKE 726

Query: 728 KNPTQSYEITFTKINGFKKSA--SFGSIQWSDGSHIVRSPIAVS 763
            N  +SY +TFT ++  K S   SFGSI+WSDG H+V SP+A+S
Sbjct: 727 ANEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Tan0022681.1 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.8e-214
Identity = 391/740 (52.84%), Postives = 503/740 (67.97%), Query Frame = 0

Query: 30  QETFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFSTRITTAQA 89
           ++T+I+ V+ S+KP  F +HH W++S L S S       LLY Y  + +GFS  + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRQMPGVISVIPDRIHQLHTTRTPHFLGLADSLGLWPETNYADDVIIGVLDTGIWPE 149
            + L     ++ +  D ++ LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCETGEGFPASACNRKIIGARAYFYGYESNLGRSLHDSSDFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G       +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFGCYDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGMVFSGVSLYSGDPLGDSKLPLVY--AGDCGNR 389
            N+APW++TVGA T+DR+F A   LG+G   +GVSLYSG  +G   L LVY       + 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKAAGGLGLILTNTEENGEELLADSHLI 449
            C  G+LDSS V GKIVVCDRG NARV KG+ V+ AGGLG+I+ NT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTPEILK 509
           P   +G+  G+ L++Y+ SD  PTA +VF+GTV+ D  P+P VAAFSSRGPN  TPEILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDINPQDY 629
           P+AIKSAL+TT+Y LD++  P+ D + +  SNP+ HG+GH+DP KAL+PGL+YDI+ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSPGNLNYPSFAVVFDSDGDEVVKYTRTV 689
           + FLCS+ Y    I   VK  S    C  K S PG LNYPSF+V+F   G  VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSAKNPTQSYEITFTKING--FKKSASFG 749
           TNVG  S +VY+V V     V ISV P+KL F +    + Y +TF    G      A FG
Sbjct: 687 TNVGAAS-SVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIQWSDGSHIVRSPIAVSIN 765
           SI WS+  H VRSP+A S N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Tan0022681.1 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 3.4e-210
Identity = 390/777 (50.19%), Postives = 519/777 (66.80%), Query Frame = 0

Query: 11  FFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLL 70
           +FF +L   S   S S  +  T+IVHV    KPS+F +H HW++S L SL+ S  P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSII 65

Query: 71  YNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL--ADSLGLWP 130
           + Y+   +GFS R+T+  A++L   P VISVIP+++  LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACNRKIIGARA 190
           E+++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + FP SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 250
           +  GYE+  G+ ++++++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +
Sbjct: 186 FCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPK 245

Query: 251 ARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAM 310
           AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+
Sbjct: 246 ARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAI 305

Query: 311 QHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFSGVSLYSGD 370
             G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G + SGVS+Y G 
Sbjct: 306 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 365

Query: 371 PLGDSKL-PLVYAGDC--GNRY----CYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKA 430
            L   ++ PLVY G    G+ Y    C  G+LD + V GKIV+CDRG N+R  KG  V+ 
Sbjct: 366 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 425

Query: 431 AGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYI------HSDPSPTATIVF 490
            GGLG+I+ N   +GE L+AD H++P T +G   G++++ YI       S   PTATIVF
Sbjct: 426 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 485

Query: 491 RGTVIGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRR 550
           +GT +G   PAP VA+FS+RGPN  TPEILKPDVIAPG+NILA W     P+ ++ D RR
Sbjct: 486 KGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 545

Query: 551 VEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSK 610
            EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SAL+TT+Y++D+SG P+ D ST  
Sbjct: 546 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 605

Query: 611 ESNPFVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVK---DSSYAQL 670
            S+   +G+GH+ P KA++PGL+YDI   DY++FLC+  Y    I    +   D   A+ 
Sbjct: 606 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 665

Query: 671 CEHKLSSPGNLNYPSFAVVFDSDGDEVVK--YTRTVTNVGDESEAVYEVKVEAPQGVEIS 730
             H     GNLNYPSF+VVF   G+  +   + RTVTNVGD S++VYE+K+  P+G  ++
Sbjct: 666 AGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 725

Query: 731 VLPNKLEFSAKNPTQSYEI----TFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSI 764
           V P KL F       S+ +    T  K++    +   G I WSDG   V SP+ V++
Sbjct: 726 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTL 771

BLAST of Tan0022681.1 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 3.3e-205
Identity = 380/784 (48.47%), Postives = 525/784 (66.96%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSP-----EDQETFIVHVSKSEKPSLFSSHHHWHSS 60
           MAN +     F F+IL S+++IF ++        ++T+++H+ KS  P  +++H  W+SS
Sbjct: 1   MANKNPLQKPFLFIIL-SINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSS 60

Query: 61  ILESLS------PSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQL 120
            + S++         +  R+LY Y+ A +G + ++T  +A  L +  GV++VIP+  ++L
Sbjct: 61  KINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYEL 120

Query: 121 HTTRTPHFLGL--ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGT 180
           HTTR+P FLGL   +S  +W E     DV++GVLDTGIWPE  SF+D G+SPVPA+W+G 
Sbjct: 121 HTTRSPTFLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGA 180

Query: 181 CETGEGFPASACNRKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGS 240
           CETG+ F    CNRKI+GAR ++ GYE+  G+ + +  ++KS RD DGHGTHTA+T AGS
Sbjct: 181 CETGKRFLKRNCNRKIVGARVFYRGYEAATGK-IDEELEYKSPRDRDGHGTHTAATVAGS 240

Query: 241 FVKNASFFQYARGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVG 300
            VK A+ F +A G ARGMA +AR+AAYK+CW  GC+ SDIL+A+DQA+ADGV ++S+S+G
Sbjct: 241 PVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG 300

Query: 301 SSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREF 360
             G    Y RDS++I  FGAM+ GV VSCSAGN GP P +  N++PWI TVGAST+DR+F
Sbjct: 301 --GGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDF 360

Query: 361 LADVILGDGMVFSGVSLYSGDPL--GDSKLPLVYAGDCGN-----RYCYSGTLDSSKVAG 420
            A V +G    F GVSLY G  +   + + PLVY G   +      +C  G LD   VAG
Sbjct: 361 PATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAG 420

Query: 421 KIVVCDRGGNARVAKGSAVKAAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLK 480
           KIV+CDRG   RV KG  VK AGG+G++LTNT  NGEEL+ADSH++P   +GE  G  +K
Sbjct: 421 KIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIK 480

Query: 481 DYIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGW 540
            Y  +    TA++   GT IG   P+P VAAFSSRGPN+ + EILKPD++APGVNILA W
Sbjct: 481 QYAMTSKKATASLEILGTRIG-IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAW 540

Query: 541 SGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYS 600
           +G  +P+SLS DPRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y 
Sbjct: 541 TGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYV 600

Query: 601 LDSSGNPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQI 660
            D+   P+ D S +  S+P+ HGAGHIDP +A +PGL+YDI PQ+Y  FLC+      Q+
Sbjct: 601 HDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQL 660

Query: 661 AVFVKDSSYAQLCEHKLS-SPGNLNYPSFAVVF-DSDGDEVVKYTRTVTNVGDESEAVYE 720
            VF K S+  + C+H L+ +PGNLNYP+ + +F ++   + +   RTVTNVG    + Y+
Sbjct: 661 KVFTKHSN--RTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YK 720

Query: 721 VKVEAPQGVEISVLPNKLEFSAKNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSP 763
           V V   +G  ++V P  L F++K+   SY +TF +     K   FG + W   +H VRSP
Sbjct: 721 VSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSP 775

BLAST of Tan0022681.1 vs. NCBI nr
Match: KAG6593557.1 (Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 714/771 (92.61%), Postives = 738/771 (95.72%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MAN  LCSS FFF+ILC V VIFSRS ++QETFIVHVSKSEKP+LFSSHHHWHSSILESL
Sbjct: 1   MANLRLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPSPHPT+LLY+YE AANGFS RIT AQAAELR++PGVISVIPDR+ QLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           ADS GLW ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL  SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
            AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGN LKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRT EILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHGAGHIDPNKALNPGLIYD+NPQDYVSFLCSIGYDSRQIAVFVKDS YAQLCEHKL+SP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDE +AVYEVKVE+PQGVEI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           KN TQSYEITFTKING K SASFGSIQWSDGSHIVRSP+AVS N+GYI+SM
Sbjct: 721 KNLTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Tan0022681.1 vs. NCBI nr
Match: XP_022964269.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])

HSP 1 Score: 1429.1 bits (3698), Expect = 0.0e+00
Identity = 713/771 (92.48%), Postives = 738/771 (95.72%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MAN HLCSS FFF+ILC V VIFSRS ++QETFIVHVSKSEKP+LFSSHHHWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPSPHPT+LLY+YE AANGFS RIT AQAAELR++PGVISVIPDR+ QLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           ADS GLW ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL  SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
            AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGN LKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRT EILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHGAGHIDPNKALNPGLIYD+NPQDYVSFLCSIGYDSRQIAVFVKDS YAQLCEHKL+SP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDE +AVYEVKVE+PQGVEI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           K+ TQSYEITFTKING K SASFGSIQWSDGSHIVRSP+AVS N+GYI+SM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Tan0022681.1 vs. NCBI nr
Match: XP_023000019.1 (subtilisin-like protease SBT1.4 [Cucurbita maxima])

HSP 1 Score: 1428.3 bits (3696), Expect = 0.0e+00
Identity = 710/771 (92.09%), Postives = 738/771 (95.72%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MAN HLCSSVFFF+ILC V VIFSRS ++QETFIVHVSKSEKP+LFSSHHHWHSSIL+SL
Sbjct: 1   MANLHLCSSVFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDSL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPSPHPT+LLYNYE AANGFS RIT AQAAELR++PGVISVIPDR+ QLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           ADS GLW ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL  SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMA+RARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS VK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
            AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGN LKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPN+RT EILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHGAGHIDPNKALNPGLIYD+NPQDYVSFLCSIGYDSRQIAVFVKDS YA+LC+HKL+SP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDE +AVYEVKVE+P+GVEI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFSA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           KN TQSYEITFTKING K SASFGSIQWSDGSHIVRSP+AVS N+GYI+SM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Tan0022681.1 vs. NCBI nr
Match: XP_023514101.1 (subtilisin-like protease SBT1.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 713/771 (92.48%), Postives = 737/771 (95.59%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MAN HLCSS FFF+ILC V VIFSRS ++QETFIVHVSKSEKP+LFSSHHHWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPSPHPT+LLY+YE AANGFS RIT AQAAELR++PGVISVIPDR+ QLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           ADS GLW ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL  SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
            AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGN LKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRT EILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHGAGHIDPNKALNPGLIYD+NPQDYVSFLCSIGYDSRQIAVFVKDS YAQLCEHKL+SP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDE +AVYEVKVE+PQGVEI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           KN TQSYEITFTKING K SASFGSIQWSDGSHIVRSP+AVS N+GYI+SM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Tan0022681.1 vs. NCBI nr
Match: XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])

HSP 1 Score: 1401.3 bits (3626), Expect = 0.0e+00
Identity = 692/771 (89.75%), Postives = 733/771 (95.07%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MA  HLCSS+FF  ILC   VIFSRSPE+QETFIVHVSKSEKPSLFSSHHHWHSSILESL
Sbjct: 1   MAKSHLCSSLFFLFILCFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPSPHPT+LLYNYERA NGFS R+T AQAA+LR +PGVISVIPDRI QLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           AD+LGLWP+TNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGF AS CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFCN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFYGY SN GRS + SSDFKSARDT+GHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWEFGC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSK+PLVY GDCG+RYCYSG+L+ SKVAGKIVVCDRGGNARVAKG AVK
Sbjct: 361 GVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
           +AGGLG++L NTEENGEELLADSHLIPGTM+GEIAGNKL+DYIHSDP+PTA IVFRGTVI
Sbjct: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRT EILKPDVIAPGVNILA WSGYSSPT L+IDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLDSSG+PIKDLSTS+ESNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHGAGHIDPNKALNPGLIYDI+PQDYVSFLCSIGYDS+QIAVFVKDSSY+QLCEHKLS+P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVF  +G++VVKYTRTVTNVGDES+ VYEVKVEAPQGVEISV+PNKL F+A
Sbjct: 661 GNLNYPSFAVVF--NGEDVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFNA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           +  T SYEITFTKI+GF ++ASFGSIQWSDGSH VRSPIAVS NTG I+SM
Sbjct: 721 EKTTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769

BLAST of Tan0022681.1 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1429.1 bits (3698), Expect = 0.0e+00
Identity = 713/771 (92.48%), Postives = 738/771 (95.72%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MAN HLCSS FFF+ILC V VIFSRS ++QETFIVHVSKSEKP+LFSSHHHWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPSPHPT+LLY+YE AANGFS RIT AQAAELR++PGVISVIPDR+ QLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           ADS GLW ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL  SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
            AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGN LKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRT EILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHGAGHIDPNKALNPGLIYD+NPQDYVSFLCSIGYDSRQIAVFVKDS YAQLCEHKL+SP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDE +AVYEVKVE+PQGVEI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           K+ TQSYEITFTKING K SASFGSIQWSDGSHIVRSP+AVS N+GYI+SM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Tan0022681.1 vs. ExPASy TrEMBL
Match: A0A6J1KIR1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3 SV=1)

HSP 1 Score: 1428.3 bits (3696), Expect = 0.0e+00
Identity = 710/771 (92.09%), Postives = 738/771 (95.72%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MAN HLCSSVFFF+ILC V VIFSRS ++QETFIVHVSKSEKP+LFSSHHHWHSSIL+SL
Sbjct: 1   MANLHLCSSVFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDSL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPSPHPT+LLYNYE AANGFS RIT AQAAELR++PGVISVIPDR+ QLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           ADS GLW ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL  SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMA+RARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS VK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
            AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGN LKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPN+RT EILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHGAGHIDPNKALNPGLIYD+NPQDYVSFLCSIGYDSRQIAVFVKDS YA+LC+HKL+SP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDE +AVYEVKVE+P+GVEI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFSA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           KN TQSYEITFTKING K SASFGSIQWSDGSHIVRSP+AVS N+GYI+SM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Tan0022681.1 vs. ExPASy TrEMBL
Match: A0A6J1JUV1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3 SV=1)

HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 690/771 (89.49%), Postives = 731/771 (94.81%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MANFH CSSVFFF  LC + VIFSRSP+ QETFIVHVSKSEKPSLFSSH+ WHSSIL+SL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
           SPS  P +LLYNYERA NGF+ RIT  QAAELR++PGV+ VIPDR+ QLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           AD+LGLWP+TNYADDVIIGVLDTGIWPE PSFSDEGLSPVPASWKGTCE GEGF A+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F+GYESN+GR LH  SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDG VFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDPLGDSKLPLVY GDCGNRYCYSG+LD SKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 AAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVI 480
           +AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGNKL+DYIHSDP+P+ATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRT EILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLD+SG+PIKDLSTS +SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSP 660
           VHG GHIDPNKALNPGLIYD+NP DYVSFLCSIGYDSRQIAVFVKDSSY+ LCEHKLS+P
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSA 720
           GNLNYPSFAVVF S+G+EVVKYTRTVTNVGDE++AVYEVKVEAPQGVEISVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           K  T+SYEITFTKI+GFK SASFGSIQWSDGSHIVRSPIAVS NT  I+SM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Tan0022681.1 vs. ExPASy TrEMBL
Match: A0A6J1GQK5 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE=3 SV=1)

HSP 1 Score: 1397.5 bits (3616), Expect = 0.0e+00
Identity = 691/772 (89.51%), Postives = 732/772 (94.82%), Query Frame = 0

Query: 1   MANFHLCSSV-FFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFH CSSV FFF  LC + VIFSRSP+ QETFIVHVSK EKPSLFSSH+ WHSSIL+S
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLG 120
           LSPS  P +LLYNYERA NGF+ RIT AQAAELR++PGV+SVIPDR+ QLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASAC 180
           LAD+LGLWP+TNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCE GEGF A+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F+GYESN+GR LH  SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDG VF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSGDPLGDSKLPLVY GDCGNRYCYSG+LD SKVAGKIVVCDRGGNARVAKGSAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KAAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTV 480
           K+AGGLG+IL NTEENGEELLADSHLIPGTMIGEIAGNKL+DYIHSDP+P+ATIVFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           I DSPP+PRVAAFSSRGPNYRT EILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLD+SG PIKDLSTS +SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSS 660
           FVHG GHIDPNKALNPGLIYD+NPQDYVSFLCSIGYDSRQIAVFVKDSSY+ LCEHKLS+
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFS 720
           PGNLNYPSFAVVF S+G+EVVKYTRTVTNVGDE++AVYEVKVEAPQGVEISVLPNKLEFS
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 AKNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
           AK  TQSYEITFTKI+GFK SASFGSIQWSDGSHIVRSPIAVS NT  I+SM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of Tan0022681.1 vs. ExPASy TrEMBL
Match: A0A1S3CGN8 (LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=LOC103500685 PE=3 SV=1)

HSP 1 Score: 1364.7 bits (3531), Expect = 0.0e+00
Identity = 680/772 (88.08%), Postives = 726/772 (94.04%), Query Frame = 0

Query: 1   MANFHL-CSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHL  SS FFF +L    ++FSRS E QET+IVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLG 120
           LSPSPHPT+LLYNYERAANGFS RITTAQA ELR++PG+ISVIPDRI QLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASAC 180
           LAD+LGLW +TNYADDVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC+TGEG  A AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYESNL  SL  SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD  VF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSGDPLGDSKLPLVY GDCG+RYCYSG+LDSSKVAG+IVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KAAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTV 480
           K+AGGLG++L NTEENGEELLADSHLIPGTM+GEIAGNKL+DYIH+DP+PTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRT EILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLDSSG+PIKDL+TS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSS 660
           FVHGAGHIDPN+ALNPGLIYD+ PQDYVSFLCSIGYDS+QIAVFVK SSY+QLCEHKLS+
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEFS 720
           PGNLNYPSF+VVFD DG EVVKYTRTVTNVGDE+E VY VKVEAPQGVEISV+PNKLEF+
Sbjct: 661 PGNLNYPSFSVVFD-DG-EVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFN 720

Query: 721 AKNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISSM 772
            +  T SYEITFTKINGFK+SASFGSIQWSDG H VRSPIAVS  TG I+SM
Sbjct: 721 KEKTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of Tan0022681.1 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 994.2 bits (2569), Expect = 5.9e-290
Identity = 497/777 (63.96%), Postives = 596/777 (76.71%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
           MA   L S  F F +L       S S +  E++IVHV +S KPSLFSSH++WH S+L SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 61  SPSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL 120
             SP P  LLY+Y RA +GFS R++  Q A LR+ P VISVIPD+  ++HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 121 ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACN 180
           + + GLW  +NY +DVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  FPAS+CN
Sbjct: 121 SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCN 180

Query: 181 RKIIGARAYFYGY-ESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           RK+IGARA++ GY     G   H + + +S RDT+GHGTHTASTAAGS V NAS +QYAR
Sbjct: 181 RKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           G A GMAS+ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DS
Sbjct: 241 GTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVF 360
           IAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG VF
Sbjct: 301 IAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           +G SLY+G+ L DS+L LVY+GDCG+R CY G L+SS V GKIV+CDRGGNARV KGSAV
Sbjct: 361 TGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAV 420

Query: 421 KAAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTV 480
           K AGG G+IL NT E+GEEL ADSHL+P TM+G  AG++++DYI +  SPTA I F GT+
Sbjct: 421 KLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTL 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG SPP+PRVAAFSSRGPN+ TP ILKPDVIAPGVNILAGW+G   PT L IDPRRV+FN
Sbjct: 481 IGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNP 600
           IISGTSMSCPHVSG+AALLRKA P WSPAAIKSALVTT+Y +++SG PI+DL+T K SN 
Sbjct: 541 IISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNS 600

Query: 601 FVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCE-HKLS 660
           F+HGAGH+DPNKALNPGL+YDI  ++YV+FLC++GY+   I VF++D +    CE  KL 
Sbjct: 601 FIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLR 660

Query: 661 SPGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEAPQGVEISVLPNKLEF 720
           + G+LNYPSF+VVF S G EVVKY R V NVG   +AVYEV V++P  VEI V P+KL F
Sbjct: 661 TAGDLNYPSFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAF 720

Query: 721 SAKNPTQSYEITFTKI-----NGFKKSASFGSIQWSDGSHIVRSPIAVSINTGYISS 771
           S +     YE+TF  +      G      FGSI+W+DG H+V+SP+AV    G + S
Sbjct: 721 SKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Tan0022681.1 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 801.2 bits (2068), Expect = 7.3e-232
Identity = 427/764 (55.89%), Postives = 536/764 (70.16%), Query Frame = 0

Query: 8   SSVFFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPT 67
           SS  FF++LC      S S  DQ T+IVH++KS+ PS F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  RLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGLAD-SLGL 127
            LLY YE A +GFSTR+T  +A  L   PGVISV+P+  ++LHTTRTP FLGL + +  L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACNRKIIGA 187
           +PE     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CE G  F AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMA 247
           R +  GYES +G  + +S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA
Sbjct: 187 RFFARGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 248 SRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 307
            RAR+A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 308 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFSGVSLYS 367
           AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G  F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 368 GDPLGDSKLPLVYAGDCGN----RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKAA 427
           G+ L D  LP +YAG+  N      C +GTL   KV GKIV+CDRG NARV KG  VKAA
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 428 GGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVIGD 487
           GG+G+IL NT  NGEEL+AD+HL+P T +GE AG+ ++ Y+ +DP+PTA+I   GTV+G 
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 488 SPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIIS 547
             P+P VAAFSSRGPN  TP ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 548 GTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPFVH 607
           GTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 608 GAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVKDSSYAQLCE-HKLSSPG 667
           GAGH+ P  A NPGLIYD+  +DY+ FLC++ Y S QI   V   +Y   C+  K  S  
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR-SVSRRNYT--CDPSKSYSVA 666

Query: 668 NLNYPSFAVVFDSDGDEVVKYTRTVTNVGDESEAVYEVKVEA-PQGVEISVLPNKLEFSA 727
           +LNYPSFAV  + DG    KYTRTVT+VG      Y VKV +   GV+ISV P  L F  
Sbjct: 667 DLNYPSFAV--NVDGVGAYKYTRTVTSVG--GAGTYSVKVTSETTGVKISVEPAVLNFKE 726

Query: 728 KNPTQSYEITFTKINGFKKSA--SFGSIQWSDGSHIVRSPIAVS 763
            N  +SY +TFT ++  K S   SFGSI+WSDG H+V SP+A+S
Sbjct: 727 ANEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Tan0022681.1 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 747.3 bits (1928), Expect = 1.2e-215
Identity = 391/740 (52.84%), Postives = 503/740 (67.97%), Query Frame = 0

Query: 30  QETFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFSTRITTAQA 89
           ++T+I+ V+ S+KP  F +HH W++S L S S       LLY Y  + +GFS  + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRQMPGVISVIPDRIHQLHTTRTPHFLGLADSLGLWPETNYADDVIIGVLDTGIWPE 149
            + L     ++ +  D ++ LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCETGEGFPASACNRKIIGARAYFYGYESNLGRSLHDSSDFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G       +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFGCYDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGMVFSGVSLYSGDPLGDSKLPLVY--AGDCGNR 389
            N+APW++TVGA T+DR+F A   LG+G   +GVSLYSG  +G   L LVY       + 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKAAGGLGLILTNTEENGEELLADSHLI 449
            C  G+LDSS V GKIVVCDRG NARV KG+ V+ AGGLG+I+ NT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMIGEIAGNKLKDYIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTPEILK 509
           P   +G+  G+ L++Y+ SD  PTA +VF+GTV+ D  P+P VAAFSSRGPN  TPEILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALVTTSYSLDSSGNPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDINPQDY 629
           P+AIKSAL+TT+Y LD++  P+ D + +  SNP+ HG+GH+DP KAL+PGL+YDI+ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYAQLCEHKLSSPGNLNYPSFAVVFDSDGDEVVKYTRTV 689
           + FLCS+ Y    I   VK  S    C  K S PG LNYPSF+V+F   G  VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGDESEAVYEVKVEAPQGVEISVLPNKLEFSAKNPTQSYEITFTKING--FKKSASFG 749
           TNVG  S +VY+V V     V ISV P+KL F +    + Y +TF    G      A FG
Sbjct: 687 TNVGAAS-SVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIQWSDGSHIVRSPIAVSIN 765
           SI WS+  H VRSP+A S N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Tan0022681.1 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 733.0 bits (1891), Expect = 2.4e-211
Identity = 390/777 (50.19%), Postives = 519/777 (66.80%), Query Frame = 0

Query: 11  FFFVILCSVSVIFSRSPEDQETFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLL 70
           +FF +L   S   S S  +  T+IVHV    KPS+F +H HW++S L SL+ S  P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSII 65

Query: 71  YNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQLHTTRTPHFLGL--ADSLGLWP 130
           + Y+   +GFS R+T+  A++L   P VISVIP+++  LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFPASACNRKIIGARA 190
           E+++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + FP SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 250
           +  GYE+  G+ ++++++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +
Sbjct: 186 FCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPK 245

Query: 251 ARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAM 310
           AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+
Sbjct: 246 ARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAI 305

Query: 311 QHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGMVFSGVSLYSGD 370
             G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G + SGVS+Y G 
Sbjct: 306 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 365

Query: 371 PLGDSKL-PLVYAGDC--GNRY----CYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKA 430
            L   ++ PLVY G    G+ Y    C  G+LD + V GKIV+CDRG N+R  KG  V+ 
Sbjct: 366 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 425

Query: 431 AGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLKDYI------HSDPSPTATIVF 490
            GGLG+I+ N   +GE L+AD H++P T +G   G++++ YI       S   PTATIVF
Sbjct: 426 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 485

Query: 491 RGTVIGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRR 550
           +GT +G   PAP VA+FS+RGPN  TPEILKPDVIAPG+NILA W     P+ ++ D RR
Sbjct: 486 KGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 545

Query: 551 VEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGNPIKDLSTSK 610
            EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SAL+TT+Y++D+SG P+ D ST  
Sbjct: 546 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 605

Query: 611 ESNPFVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQIAVFVK---DSSYAQL 670
            S+   +G+GH+ P KA++PGL+YDI   DY++FLC+  Y    I    +   D   A+ 
Sbjct: 606 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 665

Query: 671 CEHKLSSPGNLNYPSFAVVFDSDGDEVVK--YTRTVTNVGDESEAVYEVKVEAPQGVEIS 730
             H     GNLNYPSF+VVF   G+  +   + RTVTNVGD S++VYE+K+  P+G  ++
Sbjct: 666 AGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 725

Query: 731 VLPNKLEFSAKNPTQSYEI----TFTKINGFKKSASFGSIQWSDGSHIVRSPIAVSI 764
           V P KL F       S+ +    T  K++    +   G I WSDG   V SP+ V++
Sbjct: 726 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTL 771

BLAST of Tan0022681.1 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 716.5 bits (1848), Expect = 2.4e-206
Identity = 380/784 (48.47%), Postives = 525/784 (66.96%), Query Frame = 0

Query: 1   MANFHLCSSVFFFVILCSVSVIFSRSP-----EDQETFIVHVSKSEKPSLFSSHHHWHSS 60
           MAN +     F F+IL S+++IF ++        ++T+++H+ KS  P  +++H  W+SS
Sbjct: 1   MANKNPLQKPFLFIIL-SINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSS 60

Query: 61  ILESLS------PSPHPTRLLYNYERAANGFSTRITTAQAAELRQMPGVISVIPDRIHQL 120
            + S++         +  R+LY Y+ A +G + ++T  +A  L +  GV++VIP+  ++L
Sbjct: 61  KINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYEL 120

Query: 121 HTTRTPHFLGL--ADSLGLWPETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGT 180
           HTTR+P FLGL   +S  +W E     DV++GVLDTGIWPE  SF+D G+SPVPA+W+G 
Sbjct: 121 HTTRSPTFLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGA 180

Query: 181 CETGEGFPASACNRKIIGARAYFYGYESNLGRSLHDSSDFKSARDTDGHGTHTASTAAGS 240
           CETG+ F    CNRKI+GAR ++ GYE+  G+ + +  ++KS RD DGHGTHTA+T AGS
Sbjct: 181 CETGKRFLKRNCNRKIVGARVFYRGYEAATGK-IDEELEYKSPRDRDGHGTHTAATVAGS 240

Query: 241 FVKNASFFQYARGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVG 300
            VK A+ F +A G ARGMA +AR+AAYK+CW  GC+ SDIL+A+DQA+ADGV ++S+S+G
Sbjct: 241 PVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG 300

Query: 301 SSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREF 360
             G    Y RDS++I  FGAM+ GV VSCSAGN GP P +  N++PWI TVGAST+DR+F
Sbjct: 301 --GGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDF 360

Query: 361 LADVILGDGMVFSGVSLYSGDPL--GDSKLPLVYAGDCGN-----RYCYSGTLDSSKVAG 420
            A V +G    F GVSLY G  +   + + PLVY G   +      +C  G LD   VAG
Sbjct: 361 PATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAG 420

Query: 421 KIVVCDRGGNARVAKGSAVKAAGGLGLILTNTEENGEELLADSHLIPGTMIGEIAGNKLK 480
           KIV+CDRG   RV KG  VK AGG+G++LTNT  NGEEL+ADSH++P   +GE  G  +K
Sbjct: 421 KIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIK 480

Query: 481 DYIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTPEILKPDVIAPGVNILAGW 540
            Y  +    TA++   GT IG   P+P VAAFSSRGPN+ + EILKPD++APGVNILA W
Sbjct: 481 QYAMTSKKATASLEILGTRIG-IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAW 540

Query: 541 SGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYS 600
           +G  +P+SLS DPRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y 
Sbjct: 541 TGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYV 600

Query: 601 LDSSGNPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDINPQDYVSFLCSIGYDSRQI 660
            D+   P+ D S +  S+P+ HGAGHIDP +A +PGL+YDI PQ+Y  FLC+      Q+
Sbjct: 601 HDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQL 660

Query: 661 AVFVKDSSYAQLCEHKLS-SPGNLNYPSFAVVF-DSDGDEVVKYTRTVTNVGDESEAVYE 720
            VF K S+  + C+H L+ +PGNLNYP+ + +F ++   + +   RTVTNVG    + Y+
Sbjct: 661 KVFTKHSN--RTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YK 720

Query: 721 VKVEAPQGVEISVLPNKLEFSAKNPTQSYEITFTKINGFKKSASFGSIQWSDGSHIVRSP 763
           V V   +G  ++V P  L F++K+   SY +TF +     K   FG + W   +H VRSP
Sbjct: 721 VSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSP 775