Tan0020163.2 (mRNA) Snake gourd v1

Overview


Name	Tan0020163.2
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Prolyl-tRNA synthetase
Location	LG01: 1884256 .. 1889022 (-)
Sequence length	2264
RNA-Seq Expression	Tan0020163.2
Synteny	Tan0020163.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCGAAAGCGCACCATATTTGCCAACGCTCAATTGCTTACGGCCAAACCTCATCTCCCTCCTTTGCTTGCAGTTTGTGACCTCCCAGAAAGCTTCATACTTCAAACAACTCCGAAGAAATGGTAGTTTCTCTGAGACTACCTTCGCTCTCCTCTTTGCTTTCCCCAGCTTCCCCTTTCTCCTTCTCCCGGCGAGCTCTGGGGATTTCATGGCGGCAGCAACGCCCGTTCCGGTTGCCGCTGCCTGCTTCTGGATTCTCTGTGCGAAGTTCGTCCACCGGGACCGAAATCCGTGTCGACAACTCGAAGAATCGAGTTCCAGAAGGAGCTATCACGCCTCGGTCGCAGGACTTCAATGCTTGGTATTTGGATGTTATTGCCAACGCTGAGCTCGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCCTATGGCTATGCCATATGGGAAGCAATACAGGTGGTGGAAACTATATTCTCTGTTTATTTTGACTGGAACATTCGAACTTTTCTTGTATGTGTGTAATTAGATGATGATTCTTTCTGCAGGAATATTTGAATGTTAAGTTCAAGGAGACCGGTCACGAAAATGCGTACTTTCCGCAGGTGTGGCTATGCCGTTTTTAAGATTCCTATCATGAATGAGTATATGGTGGAAATGTAAAATGATGAAATTTTTTATTGACTCCAGTTTATACCATACTCATTCATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCGGAGTTAGCTCTTGTAACCATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTACGTAGACTTAGCTAAGCTATTGGCCCCTTTCCAGTTTTTCATTACTCATTAGAAATATTAACCCTATTATATTTGATACTTTGCATTGGCTAAATTGGAAATGGATATTAGTCCTCAATAACAGTGACCTGAGGTTTGGTTTTGATTATTGGATTGCCTGAGGCTTTGGCAGGTTCGACCGACGAGTGAAACCATTGTGAACCACATGTTTACTCAGTGGATTCATAGTTACCGCGATCTTCCTTTGATGATTAATCAGGTTCGATGATTTGCCACATTATGGAAAAGTCTATCATTATCTTTGGCTTTTATGTTATCCTTGTTCATTACCTATTATTTTGTGTATTTGGTGTAGTGGGCAAATGTTACCAGATGGGAGATGAGGACAAAACCATTTGTAAGAACTCTTGAATTTCTGTGGCAGGAGGGTCATACTGCGCATGCCAGTCCAGAAGAGGCAGAAAAAGAGGTATATCTGATGCATCCAAGACTATTTTGTATCCTTTTTTTTAATCATTTCCTCTTCGGCTAAGGATCTTGATGCTGCATTTGCAGGCTATTCAGATGATTGACATATATACAAAATTTTCTTATGAGCAAGCGGCAATACCTGTTGTTGCAGGACGCAAATCAAAGGTGGAGACATTTGCTGGTGCGGACAAGACCTATACAATAGAGGCCATGATGGGGGATCGAAAAGCACTGCAGGCAGGAACCAGCCATAACCTTGGGCAGAACTTTTCTCGTGCTTTTGGAACACAGGTATTTTGGCAAATCAATGAAATTGCTTCCGACTTAAATGAACCTGTTATAATAGTTGAGATATATTTTTTATTTCATTGTGGCACATGTGTGATTCTTGTTCCTGTTGCACTTTGGCTTCAAATTGATTTCTAAATTCTGTTTGGTTTGTTAATATGGTTCATAAATGCCCTTTTGAAACATTCAACCAAAACATTATTTGATATGTCTGGCATATTATATTGCACTGTCAATGTGATTGTGTTACTGAAACATTTACTTGAGTAGTTCACTGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCATGGGCAATTAGTACACGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTTGCTCCAATACAGGTATTAATTTAAATTTATACTTTTAATTTTGTAGAGCATTACTATTTGTTAATTCTAGAGCTTAGGAGCTGTGCAAGATAAAAGTTTCATACATAGTCTTGAATGAGAGAAAAATGTGAGGAATCCTCTTTTCTACTCTGACTCTCCCACCCTGGTGCTTTTCTTTTCGTGCCTTAATTTTCAGTTTCATCTAGAAACTTATATAGAATCCTTGAAAAGAGGAAGTATATTTAATGATAGTGTGATGTAAAAGTGACTAGAGGTTAAATGCATAACATACTTTTCTTATGTCCAACTCTTTGAAAACTTGAAAATTGTAGAAAGAATTTCTGAAATGTCGGCATGTTTCAGTTCTTTTAGTCCTCCGGTTCATATTATTCCTTTTTAACAAATATCTTTAAATGTGCCTGACTTATATCTAGGTGGTAATCATACCCATTTGGAAGAAGGAGGATGATAAAGCAGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGTAAGCCTTCAGACTGCAGGGATAAAGGTTAAACTTGATGAGTCTGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGTTGATGCTTAACTGCTTATTTTTTATGATTAAGATCAATTTCTGACAGTTCTATTCCCCTCAGTTGTGGGAAAATATTGTCTAAAAAACAAACACTCATGTGAAAATTTTCATCATAGACTGGTTATCTGCCAATAGATATTCGTGATCTCTCTCTGTTATGAATACTCTGCACCCCAAACACAGACTATTATAACCCCAGACTATACTACTTTGCATTTTAAATAATATTATATGATTCTACAGATTATAATAACCCACATATTATTATAACCCATTGTTATTATAACCTACTCAGCGCCTCAAACAATCTCTTTATGATTATTTTCACAACTTGGAATCTAGTTTGTGAAAAGAATATTCTTCTGTAGTGTAGGTTGGTTGGCTGACATTTTTCTTTCCAGGATGTATGCATGTTTTCCAGGATGTATGCATGTTGGTTATTGATAGTTTTAAATGTTCCAATTCCTTTTTAGCTCCATGATTTTCACCTGGATCTTGTCTTCCTCCCACTTTGCATTTGTTGCTATTAACTTGAAATCAAATGTCTGTAACTAGACTTGGATTTATAGGGCGTTCCTTTGAGGATTGAGATTGGTCCTAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGATATTCCTGGAAAGCAAGGGAAAGTTTTTGGGATATCCATGGAACCTTCTATTTTAGAAGCTTATGTGAAAGAGACGTTGGATGAGATCCAATCATCTCTTCTTGAACAAGCAAAAACTTTTCGTGACAGGTGCTTTCTTGTTTTCTTATGTTGGGATGTTTCTTGATAAGTATGTAAATCTACGTTCAATTACATTACATTTATCCCTTGCGAATTGAAGCAATTGAATGTGAGTAAGCATGCTTCTTGGAAATTGTAGTAAGTGGTTAAATGTAAATACATTCGGAATTGTGGGCTTCAAGAAAATTGTTCCCATCTCCACCCTCATTTTCTTCCTGTCCCTGCTTCTCCATAATTTCTTCCCCTTCTTTTTTTCACTAGATTCATGTAAGGAAAAAATTCTCTTAATGTTTCATTATATTTGCAAGTAAAGAAGGGATGGGGAGTGAATGGTGTGCTTCACAGTTCTCATTTCAATAATTATTGTGGCAATCATATTCTTGATCATTGAAGTCATGAATAGTGGCTTAATGCATTTTGTGCATTTTTTCATCTAAAACAAAGAAAATTGTGGTTAATCTGGCAGCATGATTAATTTTGTGTAACAGTGAATCAGCTCTCAAGCTGTCACGGGACTGAAATGTTTTTTAATATACTTTTGATAATTATTTTATCTTAGTATATGAAAAACCTGCATTCACGCCCTTTTCATAAGATATACATTATTTTGTTAACGACCTCCTTTTTGCAGTAACATTGTGGATGTGAGTTCATATAGTGAACTTAAAGAGGCAATCTCCCAGGGGAAATGGGCTAGGGGTCCCTGGTCAGCTAGGTATGCTTATTTTCATTTCTTGGGATTTGTGGATAAATTTTTTAATTTTGTATGTTTGTGCACAATTTTGCTTGCTTATGTATGGTCTTCATCGTTTTTGCAGTGACCAAGATGAATTGAAAGTTAAAGAAGAAACAGGAGCAACAATTAGGTGCTTTCCTTTTGAACAGCCACAAGGGCCTAAAACATGCTTGATGACCGGTAACGCTGCTGAGGAAGTTGCCATTTTTGCAAAGTCTTACTGAGATATAATTTCAAGGCATGACTTAAAAGCCGCCAAGTCTTATTCTTTTGGCCCTATAATCACATTGTGCACTCTCTTTAAGGTCGTTAAATTTTCCCGAAAAGGAGCCTCCTCTATCAACTCTTCCAAGGCAAAGGCTTTAGCTACCTGGTTAGCATTGACATTTATTTTCTGAAGTTGGACGGGTTTTGTTCTCTGCCTGCCTAATTCTTTTGTTTGGTGGCTCAATCCTTCATTTTTGGAGCAGTATTTAAGCGATGGATTATGTATAATTAACAATCTCGTTAAAGCATAACTTAGTTCTTGTCTCTGTTTCATGTTTCATAACACGGGCTTGCTATTTCAATTTTAAGATGGTCGTGCAAACTACCTAATATAGTGTTATTTTGTTGAATATTTGATCCTTTAGTAATAATTTTGCCATTTCTTCTGTCCAAGGAAACAATTTTAGTATTATAATACAAAAGTTATTTGTTTGTATTCTCA

mRNA sequence

GTCGAAAGCGCACCATATTTGCCAACGCTCAATTGCTTACGGCCAAACCTCATCTCCCTCCTTTGCTTGCAGTTTGTGACCTCCCAGAAAGCTTCATACTTCAAACAACTCCGAAGAAATGGTAGTTTCTCTGAGACTACCTTCGCTCTCCTCTTTGCTTTCCCCAGCTTCCCCTTTCTCCTTCTCCCGGCGAGCTCTGGGGATTTCATGGCGGCAGCAACGCCCGTTCCGGTTGCCGCTGCCTGCTTCTGGATTCTCTGTGCGAAGTTCGTCCACCGGGACCGAAATCCGTGTCGACAACTCGAAGAATCGAGTTCCAGAAGGAGCTATCACGCCTCGGTCGCAGGACTTCAATGCTTGGTATTTGGATGTTATTGCCAACGCTGAGCTCGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCCTATGGCTATGCCATATGGGAAGCAATACAGGAATATTTGAATGTTAAGTTCAAGGAGACCGGTCACGAAAATGCGTACTTTCCGCAGTTTATACCATACTCATTCATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCGGAGTTAGCTCTTGTAACCATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTTCGACCGACGAGTGAAACCATTGTGAACCACATGTTTACTCAGTGGATTCATAGTTACCGCGATCTTCCTTTGATGATTAATCAGTGGGCAAATGTTACCAGATGGGAGATGAGGACAAAACCATTTGTAAGAACTCTTGAATTTCTGTGGCAGGAGGGTCATACTGCGCATGCCAGTCCAGAAGAGGCAGAAAAAGAGGCTATTCAGATGATTGACATATATACAAAATTTTCTTATGAGCAAGCGGCAATACCTGTTGTTGCAGGACGCAAATCAAAGGTGGAGACATTTGCTGGTGCGGACAAGACCTATACAATAGAGGCCATGATGGGGGATCGAAAAGCACTGCAGGCAGGAACCAGCCATAACCTTGGGCAGAACTTTTCTCGTGCTTTTGGAACACAGTTCACTGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCATGGGCAATTAGTACACGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTTGCTCCAATACAGGTGGTAATCATACCCATTTGGAAGAAGGAGGATGATAAAGCAGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGTAAGCCTTCAGACTGCAGGGATAAAGGTTAAACTTGATGAGTCTGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGACTTGGATTTATAGGGCGTTCCTTTGAGGATTGAGATTGGTCCTAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGATATTCCTGGAAAGCAAGGGAAAGTTTTTGGGATATCCATGGAACCTTCTATTTTAGAAGCTTATGTGAAAGAGACGTTGGATGAGATCCAATCATCTCTTCTTGAACAAGCAAAAACTTTTCGTGACAGTAACATTGTGGATGTGAGTTCATATAGTGAACTTAAAGAGGCAATCTCCCAGGGGAAATGGGCTAGGGGTCCCTGGTCAGCTAGTGACCAAGATGAATTGAAAGTTAAAGAAGAAACAGGAGCAACAATTAGGTGCTTTCCTTTTGAACAGCCACAAGGGCCTAAAACATGCTTGATGACCGGTAACGCTGCTGAGGAAGTTGCCATTTTTGCAAAGTCTTACTGAGATATAATTTCAAGGCATGACTTAAAAGCCGCCAAGTCTTATTCTTTTGGCCCTATAATCACATTGTGCACTCTCTTTAAGGTCGTTAAATTTTCCCGAAAAGGAGCCTCCTCTATCAACTCTTCCAAGGCAAAGGCTTTAGCTACCTGGTTAGCATTGACATTTATTTTCTGAAGTTGGACGGGTTTTGTTCTCTGCCTGCCTAATTCTTTTGTTTGGTGGCTCAATCCTTCATTTTTGGAGCAGTATTTAAGCGATGGATTATGTATAATTAACAATCTCGTTAAAGCATAACTTAGTTCTTGTCTCTGTTTCATGTTTCATAACACGGGCTTGCTATTTCAATTTTAAGATGGTCGTGCAAACTACCTAATATAGTGTTATTTTGTTGAATATTTGATCCTTTAGTAATAATTTTGCCATTTCTTCTGTCCAAGGAAACAATTTTAGTATTATAATACAAAAGTTATTTGTTTGTATTCTCA

Coding sequence (CDS)

ATGGTAGTTTCTCTGAGACTACCTTCGCTCTCCTCTTTGCTTTCCCCAGCTTCCCCTTTCTCCTTCTCCCGGCGAGCTCTGGGGATTTCATGGCGGCAGCAACGCCCGTTCCGGTTGCCGCTGCCTGCTTCTGGATTCTCTGTGCGAAGTTCGTCCACCGGGACCGAAATCCGTGTCGACAACTCGAAGAATCGAGTTCCAGAAGGAGCTATCACGCCTCGGTCGCAGGACTTCAATGCTTGGTATTTGGATGTTATTGCCAACGCTGAGCTCGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCCTATGGCTATGCCATATGGGAAGCAATACAGGAATATTTGAATGTTAAGTTCAAGGAGACCGGTCACGAAAATGCGTACTTTCCGCAGTTTATACCATACTCATTCATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCGGAGTTAGCTCTTGTAACCATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTTCGACCGACGAGTGAAACCATTGTGAACCACATGTTTACTCAGTGGATTCATAGTTACCGCGATCTTCCTTTGATGATTAATCAGTGGGCAAATGTTACCAGATGGGAGATGAGGACAAAACCATTTGTAAGAACTCTTGAATTTCTGTGGCAGGAGGGTCATACTGCGCATGCCAGTCCAGAAGAGGCAGAAAAAGAGGCTATTCAGATGATTGACATATATACAAAATTTTCTTATGAGCAAGCGGCAATACCTGTTGTTGCAGGACGCAAATCAAAGGTGGAGACATTTGCTGGTGCGGACAAGACCTATACAATAGAGGCCATGATGGGGGATCGAAAAGCACTGCAGGCAGGAACCAGCCATAACCTTGGGCAGAACTTTTCTCGTGCTTTTGGAACACAGTTCACTGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCATGGGCAATTAGTACACGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTTGCTCCAATACAGGTGGTAATCATACCCATTTGGAAGAAGGAGGATGATAAAGCAGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGTAAGCCTTCAGACTGCAGGGATAAAGGTTAAACTTGATGAGTCTGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGACTTGGATTTATAGGGCGTTCCTTTGA

Protein sequence

MVVSLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNVKFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMKTWIYRAFL

Homology

[Close]

BLAST of Tan0020163.2 vs. ExPASy Swiss-Prot
Match: Q9FYR6 (Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA6 PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 7.5e-188
Identity = 322/405 (79.51%), Postives = 357/405 (88.15%), Query Frame = 0


Query: 4   SLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVDNSK 63
           SLRLPSL+SLL PA+    +     +  R +       P SGF+   S T +     +  
Sbjct: 5   SLRLPSLTSLLFPATTRYPATLRRTVCLRNR-------PLSGFATAPSGTASPETKSSEV 64

Query: 64  NRV-PEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNVKF 123
           +R+  + A+TPRSQDFNAWYLDVIA+AELADYGPVRGTMVIRPYGYAIWEAIQ+YLNVKF
Sbjct: 65  DRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKF 124

Query: 124 KETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNH 183
           KETGH N YFPQFIPYSFIEKEASHVEGFSPELALVT+GGGKELEEKLVVRPTSETIVNH
Sbjct: 125 KETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSETIVNH 184

Query: 184 MFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQM 243
           MFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLEFLWQEGHTAHA+PEEAEKEA QM
Sbjct: 185 MFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLEFLWQEGHTAHATPEEAEKEAKQM 244

Query: 244 IDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSR 303
           I+IYT+F++EQ AIPV+ GRKSK+ETFAGAD TYTIEAMMGDRKALQAGTSHNLGQNFSR
Sbjct: 245 IEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDRKALQAGTSHNLGQNFSR 304

Query: 304 AFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKK 363
           AFGTQF DENG+RQHVWQTSWA+STRFVGGIIMTHGDD GLMLPPK+APIQVVI+PIWKK
Sbjct: 305 AFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVPIWKK 364

Query: 364 EDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           + +K  VLSAASSVK +LQTAG++VKLD+++QRTPGWKFNFWEMK
Sbjct: 365 DTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMK 402

[Close]

BLAST of Tan0020163.2 vs. ExPASy Swiss-Prot
Match: Q72GF9 (Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=proS PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 4.4e-127
Identity = 217/348 (62.36%), Postives = 276/348 (79.31%), Query Frame = 0


Query: 68  EGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNVKFKETGH 127
           E  +TP+SQDF+ WYL+VI  AELADYGPVRGT+V+RPYGYAIWE IQ+ L+  FKETGH
Sbjct: 4   EKGLTPQSQDFSEWYLEVIQKAELADYGPVRGTIVVRPYGYAIWENIQQVLDRMFKETGH 63

Query: 128 ENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQW 187
           +NAYFP FIP SF+ KEA HVEGFSPELA+VT  GG+ELEE L VRPTSET++ +M+++W
Sbjct: 64  QNAYFPLFIPMSFLRKEAEHVEGFSPELAVVTHAGGEELEEPLAVRPTSETVIGYMWSKW 123

Query: 188 IHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYT 247
           I S+RDLP ++NQW NV RWEMRT+PF+RT EFLWQEGHTAHA+ EEAE+E  +M+ IY 
Sbjct: 124 IRSWRDLPQLLNQWGNVVRWEMRTRPFLRTSEFLWQEGHTAHATREEAEEEVRRMLSIYA 183

Query: 248 KFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQ 307
           + + E AAIPV+ G K++ E FAGA  T TIEA+M D KALQAGTSH LG+NF+RAF  +
Sbjct: 184 RLAREYAAIPVIEGLKTEKEKFAGAVYTTTIEALMKDGKALQAGTSHYLGENFARAFDIK 243

Query: 308 FTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKEDDKA 367
           F D + Q ++V  TSW +S RF+G IIMTHGDD GL+LPP+LAPIQVVI+PI+ K++ + 
Sbjct: 244 FQDRDLQVKYVHTTSWGLSWRFIGAIIMTHGDDRGLVLPPRLAPIQVVIVPIY-KDESRE 303

Query: 368 EVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMKTWIYRAFL 416
            VL AA  ++ +LQ  G++V LD+ +Q TPG+KF+ WE+K   +R  L
Sbjct: 304 RVLEAAQGLRQALQAKGLRVHLDDRDQHTPGYKFHEWELKGVPFRVEL 350

[Close]

BLAST of Tan0020163.2 vs. ExPASy Swiss-Prot
Match: Q5SM28 (Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=proS PE=1 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.8e-126
Identity = 216/348 (62.07%), Postives = 275/348 (79.02%), Query Frame = 0


Query: 68  EGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNVKFKETGH 127
           E  +TP+SQDF+ WYL+VI  AELADYGPVRGT+V+RPYGYAIWE IQ+ L+  FKETGH
Sbjct: 4   EKGLTPQSQDFSEWYLEVIQKAELADYGPVRGTIVVRPYGYAIWENIQQVLDRMFKETGH 63

Query: 128 ENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQW 187
           +NAYFP FIP SF+ KEA HVEGFSPELA+VT  GG+ELEE L VRPTSET++ +M+++W
Sbjct: 64  QNAYFPLFIPMSFLRKEAEHVEGFSPELAVVTHAGGEELEEPLAVRPTSETVIGYMWSKW 123

Query: 188 IHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYT 247
           I S+RDLP ++NQW NV RWEMRT+PF+RT EFLWQEGHTAHA+ EEAE+E  +M+ IY 
Sbjct: 124 IRSWRDLPQLLNQWGNVVRWEMRTRPFLRTSEFLWQEGHTAHATREEAEEEVRRMLSIYA 183

Query: 248 KFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQ 307
           + + E AAIPV+ G K++ E FAGA  T TIEA+M D KALQAGTSH LG+NF+RAF  +
Sbjct: 184 RLAREYAAIPVIEGLKTEKEKFAGAVYTTTIEALMKDGKALQAGTSHYLGENFARAFDIK 243

Query: 308 FTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKEDDKA 367
           F D + Q ++V  TSW +S RF+G IIMTHGDD GL+LPP+LAPIQVVI+PI+ K++ + 
Sbjct: 244 FQDRDLQVKYVHTTSWGLSWRFIGAIIMTHGDDRGLVLPPRLAPIQVVIVPIY-KDESRE 303

Query: 368 EVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMKTWIYRAFL 416
            VL AA  ++ +L   G++V LD+ +Q TPG+KF+ WE+K   +R  L
Sbjct: 304 RVLEAAQGLRQALLAQGLRVHLDDRDQHTPGYKFHEWELKGVPFRVEL 350

[Close]

BLAST of Tan0020163.2 vs. ExPASy Swiss-Prot
Match: B3QPX3 (Proline--tRNA ligase OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=proS PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.7e-120
Identity = 209/337 (62.02%), Postives = 255/337 (75.67%), Query Frame = 0


Query: 71  ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNVKFKETGHENA 130
           IT R QD++ WY+D++ +A+LADY  VRG MVIRP GYAIWE +Q  L+  FKETGH NA
Sbjct: 5   ITSRQQDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKETGHVNA 64

Query: 131 YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQWIHS 190
           YFP FIP SFI KEA H+EGF+PE A+VT GGG+EL EKL VRPTSETI+   + +WI S
Sbjct: 65  YFPMFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQS 124

Query: 191 YRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFS 250
           YRDLPL+INQWANV RWEMRT+ F+RT EFLWQEGHTAHA PEEA++E I+MI++Y  F+
Sbjct: 125 YRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHADPEEAQEEVIRMINVYRTFA 184

Query: 251 YEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTD 310
            E  A+PV+ G+KS+ E FAGAD TY IEAMM D KALQAGTSHNLGQNF++AF  QF  
Sbjct: 185 EEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDCQFQT 244

Query: 311 ENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKEDDKAEVL 370
           + G   +VW TSW +STR +G +IM H DD GL+LPPKLA  QVVIIPI K   DK EV 
Sbjct: 245 KEGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILK--GDKEEVR 304

Query: 371 SAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           + A  +  +L   GI   +D+SE  +PGWKF  +E++
Sbjct: 305 AHARFIAKALNKHGISTFVDDSENNSPGWKFAEYELQ 339

[Close]

BLAST of Tan0020163.2 vs. ExPASy Swiss-Prot
Match: Q3B2T0 (Proline--tRNA ligase OS=Pelodictyon luteolum (strain DSM 273 / 2530) OX=319225 GN=proS PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 8.8e-120
Identity = 206/337 (61.13%), Postives = 261/337 (77.45%), Query Frame = 0


Query: 71  ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNVKFKETGHENA 130
           IT R++D++ WY+D++ +A+LADY  VRG MVIRP GYAIWE +Q  L+  FKETGH NA
Sbjct: 5   ITARNEDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDRMFKETGHVNA 64

Query: 131 YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQWIHS 190
           YFP FIP SFI KEA H+EGF+PE A+VT GGG+EL EKL VRPTSETI+   + +WI S
Sbjct: 65  YFPLFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQS 124

Query: 191 YRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFS 250
           YRDLP++INQWANV RWEMRT+ F+RT EFLWQEGHTAHA+PEE+++E ++MI++Y  F+
Sbjct: 125 YRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHATPEESQEEVMRMINVYRTFA 184

Query: 251 YEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTD 310
            E  A+PV+ GRK+  E FAGA++TY IEAMM D KALQAGTSHNLGQNF++AF  QF  
Sbjct: 185 EEYMAMPVIMGRKTDSEKFAGAEETYCIEAMMQDGKALQAGTSHNLGQNFAKAFDCQFQT 244

Query: 311 ENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKEDDKAEVL 370
           ++ +  +VW TSW +STR +G +IM H DD GL+LPPKLA  QVVIIPI +   DKA VL
Sbjct: 245 KDSRLDYVWATSWGVSTRLIGALIMAHSDDRGLVLPPKLASRQVVIIPILR--GDKAAVL 304

Query: 371 SAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
             A ++  SL+ AGI   +D SEQ +PGWKF  +E++
Sbjct: 305 EQADAMAASLKAAGIPAFVDGSEQNSPGWKFAEYELQ 339

[Close]

BLAST of Tan0020163.2 vs. NCBI nr
Match: XP_023541480.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793.9 bits (2049), Expect = 6.7e-226
Identity = 389/407 (95.58%), Postives = 400/407 (98.28%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVD 60
           MVVSLRLPSLSSLLSPASPF+FSRRALGISWRQQRPFRLPL A GFSVRSSSTGTEI VD
Sbjct: 1   MVVSLRLPSLSSLLSPASPFTFSRRALGISWRQQRPFRLPLLAPGFSVRSSSTGTEIHVD 60

Query: 61  NSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120
           NSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV
Sbjct: 61  NSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120

Query: 121 KFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180
           KFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV
Sbjct: 121 KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180

Query: 181 NHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240
           NHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI
Sbjct: 181 NHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240

Query: 241 QMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300
           QMIDIYTKF+YEQAAIPV+AGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF
Sbjct: 241 QMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300

Query: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIW 360
           SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPP+LAPIQVVIIPIW
Sbjct: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVIIPIW 360

Query: 361 KKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           KKE  KAEVL+AA+SV+V+L+TAGIKVKLD SEQRTPGWKFNFWEMK
Sbjct: 361 KKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTPGWKFNFWEMK 407

[Close]

BLAST of Tan0020163.2 vs. NCBI nr
Match: XP_022945172.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 792.0 bits (2044), Expect = 2.6e-225
Identity = 387/407 (95.09%), Postives = 400/407 (98.28%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVD 60
           MVVSLRLPSLSSLLSPASPF+FSRRALGISWRQQRPFRLPL A G SVRSSSTGTEI VD
Sbjct: 1   MVVSLRLPSLSSLLSPASPFTFSRRALGISWRQQRPFRLPLLAPGLSVRSSSTGTEIHVD 60

Query: 61  NSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120
           NSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV
Sbjct: 61  NSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120

Query: 121 KFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180
           KFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV
Sbjct: 121 KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180

Query: 181 NHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240
           NHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI
Sbjct: 181 NHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240

Query: 241 QMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300
           QMIDIYTKF+YEQAAIPV+AGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF
Sbjct: 241 QMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300

Query: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIW 360
           SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPP+LAPIQVVI+PIW
Sbjct: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVIVPIW 360

Query: 361 KKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           KKE  KAEVL+AA+SV+V+L+TAGIKVKLD+SEQRTPGWKFNFWEMK
Sbjct: 361 KKEGVKAEVLNAAASVEVNLRTAGIKVKLDDSEQRTPGWKFNFWEMK 407

[Close]

BLAST of Tan0020163.2 vs. NCBI nr
Match: XP_038878181.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 785.8 bits (2028), Expect = 1.8e-223
Identity = 388/411 (94.40%), Postives = 396/411 (96.35%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASP----FSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTE 60
           MVVSLRLP +SSLLSPAS     F+FSRRALGISWRQQRP RLPL +SGFS RSSSTGTE
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNFTFSRRALGISWRQQRPLRLPLLSSGFSARSSSTGTE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQE 120
           IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120

Query: 121 YLNVKFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           +LN KFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 FLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLEFLWQEGHTAHASPEEAE
Sbjct: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLEFLWQEGHTAHASPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMID+YTKFSYEQAAIPV+AGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDVYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI
Sbjct: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360

Query: 361 IPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           IPIWKK+ DK EVLSAASSVK SLQTAGIKVKLDESEQRTPGWKFNFWEMK
Sbjct: 361 IPIWKKDGDKVEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMK 411

[Close]

BLAST of Tan0020163.2 vs. NCBI nr
Match: KAG7013253.1 (Proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 785.0 bits (2026), Expect = 3.1e-223
Identity = 387/414 (93.48%), Postives = 400/414 (96.62%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVD 60
           MVVSLRLPSLSSLLSPASPF+FSRRALGISWRQQRPFRLPL A G SVRSSSTGTEI VD
Sbjct: 1   MVVSLRLPSLSSLLSPASPFTFSRRALGISWRQQRPFRLPLLAPGLSVRSSSTGTEIHVD 60

Query: 61  NSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120
           NSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV
Sbjct: 61  NSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120

Query: 121 KFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180
           KFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV
Sbjct: 121 KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180

Query: 181 NHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKE-- 240
           NHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKE  
Sbjct: 181 NHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEEF 240

Query: 241 -----AIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300
                AIQMIDIYTKF+YEQAAIPV+AGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS
Sbjct: 241 DAALQAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300

Query: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQ 360
           HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPP+LAPIQ
Sbjct: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQ 360

Query: 361 VVIIPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           VVI+PIWKKE  KAEVL+AA+SV+V+L+TAGIKVKLD+SEQRTPGWKFNFWEMK
Sbjct: 361 VVIVPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDDSEQRTPGWKFNFWEMK 414

[Close]

BLAST of Tan0020163.2 vs. NCBI nr
Match: XP_022968367.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 784.6 bits (2025), Expect = 4.1e-223
Identity = 386/407 (94.84%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVD 60
           MVVSLRLPSLSSLLSPASPF+FSRRALGIS RQQRPFRLPL A  FSVRSSSTGTEI VD
Sbjct: 1   MVVSLRLPSLSSLLSPASPFTFSRRALGISLRQQRPFRLPLLAPRFSVRSSSTGTEIHVD 60

Query: 61  NSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120
           NSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV
Sbjct: 61  NSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120

Query: 121 KFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180
           KFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV
Sbjct: 121 KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180

Query: 181 NHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240
           NHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI
Sbjct: 181 NHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240

Query: 241 QMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300
           QMIDIYTKF+YEQAAIPV+AGRKSK+ETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF
Sbjct: 241 QMIDIYTKFAYEQAAIPVIAGRKSKMETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300

Query: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIW 360
           SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPP+LAPIQVVIIPIW
Sbjct: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVIIPIW 360

Query: 361 KKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           KKE  KAEVL+AA+SV+V+L+TAGIKVKLD SEQRTPGWKFNFWEMK
Sbjct: 361 KKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTPGWKFNFWEMK 407

[Close]

BLAST of Tan0020163.2 vs. ExPASy TrEMBL
Match: A0A6J1G067 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111449493 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.2e-225
Identity = 387/407 (95.09%), Postives = 400/407 (98.28%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVD 60
           MVVSLRLPSLSSLLSPASPF+FSRRALGISWRQQRPFRLPL A G SVRSSSTGTEI VD
Sbjct: 1   MVVSLRLPSLSSLLSPASPFTFSRRALGISWRQQRPFRLPLLAPGLSVRSSSTGTEIHVD 60

Query: 61  NSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120
           NSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV
Sbjct: 61  NSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120

Query: 121 KFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180
           KFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV
Sbjct: 121 KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180

Query: 181 NHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240
           NHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI
Sbjct: 181 NHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240

Query: 241 QMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300
           QMIDIYTKF+YEQAAIPV+AGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF
Sbjct: 241 QMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300

Query: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIW 360
           SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPP+LAPIQVVI+PIW
Sbjct: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVIVPIW 360

Query: 361 KKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           KKE  KAEVL+AA+SV+V+L+TAGIKVKLD+SEQRTPGWKFNFWEMK
Sbjct: 361 KKEGVKAEVLNAAASVEVNLRTAGIKVKLDDSEQRTPGWKFNFWEMK 407

[Close]

BLAST of Tan0020163.2 vs. ExPASy TrEMBL
Match: A0A6J1HTC6 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111467631 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.0e-223
Identity = 386/407 (94.84%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVD 60
           MVVSLRLPSLSSLLSPASPF+FSRRALGIS RQQRPFRLPL A  FSVRSSSTGTEI VD
Sbjct: 1   MVVSLRLPSLSSLLSPASPFTFSRRALGISLRQQRPFRLPLLAPRFSVRSSSTGTEIHVD 60

Query: 61  NSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120
           NSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV
Sbjct: 61  NSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNV 120

Query: 121 KFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180
           KFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV
Sbjct: 121 KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIV 180

Query: 181 NHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240
           NHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI
Sbjct: 181 NHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAI 240

Query: 241 QMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300
           QMIDIYTKF+YEQAAIPV+AGRKSK+ETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF
Sbjct: 241 QMIDIYTKFAYEQAAIPVIAGRKSKMETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNF 300

Query: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIW 360
           SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPP+LAPIQVVIIPIW
Sbjct: 301 SRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVIIPIW 360

Query: 361 KKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           KKE  KAEVL+AA+SV+V+L+TAGIKVKLD SEQRTPGWKFNFWEMK
Sbjct: 361 KKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTPGWKFNFWEMK 407

[Close]

BLAST of Tan0020163.2 vs. ExPASy TrEMBL
Match: A0A5D3CUH7 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2047G00090 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 6.8e-216
Identity = 379/412 (91.99%), Postives = 391/412 (94.90%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASP----FSFSRRALGISWRQQR-PFRLPLPASGFSVRSSSTGT 60
           MVVSLRLP +SSLLSPAS     F+FSRRALGISWR QR P RLPL +S FS RSSST T
Sbjct: 1   MVVSLRLPLISSLLSPASSRSPNFAFSRRALGISWRHQRPPLRLPLLSSPFSPRSSSTNT 60

Query: 61  EIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 120
           EIR ++SKNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ
Sbjct: 61  EIRTESSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 120

Query: 121 EYLNVKFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT 180
           EYLNVKFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT
Sbjct: 121 EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT 180

Query: 181 SETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA 240
           SETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA
Sbjct: 181 SETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA 240

Query: 241 EKEAIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN 300
           EKEAIQMIDIYTKFSYEQAAIPV+AGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN
Sbjct: 241 EKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN 300

Query: 301 LGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVV 360
           LGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVV
Sbjct: 301 LGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVV 360

Query: 361 IIPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           IIPIWKK+ DKAEVL+AASSVK  LQTAG+KVKLDES+Q+T GWKFNFWEMK
Sbjct: 361 IIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQKTAGWKFNFWEMK 412

[Close]

BLAST of Tan0020163.2 vs. ExPASy TrEMBL
Match: A0A0A0L6I6 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G213510 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 9.8e-215
Identity = 377/414 (91.06%), Postives = 389/414 (93.96%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPASP----FSFSRRALGISWRQQR---PFRLPLPASGFSVRSSST 60
           MVVSLRLP +SSLLSPAS      +FSRRALGISWR QR   P R PL +S FS RSSST
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNLAFSRRALGISWRHQRQRPPLRFPLLSSPFSPRSSST 60

Query: 61  GTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120
            TEI +D+SKNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA
Sbjct: 61  NTEIHIDSSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120

Query: 121 IQEYLNVKFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR 180
           IQEYLNVKFKETGHEN YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR
Sbjct: 121 IQEYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR 180

Query: 181 PTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE 240
           PTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE
Sbjct: 181 PTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE 240

Query: 241 EAEKEAIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300
           EAEKEAIQMIDIYTKFSYEQAAIPV+AGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS
Sbjct: 241 EAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300

Query: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQ 360
           HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQ
Sbjct: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQ 360

Query: 361 VVIIPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           VVIIPIWKK+ DKAEVL+AASSVK  LQTAG+KVKLDES+Q+T GWKFNFWEMK
Sbjct: 361 VVIIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQKTAGWKFNFWEMK 414

[Close]

BLAST of Tan0020163.2 vs. ExPASy TrEMBL
Match: A0A6J1C8A3 (Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.6e-211
Identity = 369/411 (89.78%), Postives = 385/411 (93.67%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLLSPAS----PFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTE 60
           MVVSLRLPSLS+L SPAS      +F RRA      QQR F LP  A+G+S +SSST TE
Sbjct: 1   MVVSLRLPSLSALFSPASTTTTSTTFFRRAPVTLRPQQRLFHLPRLAAGYSAQSSSTETE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQE 120
           IR DNSKNRVPEGAITPRS+DFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IRADNSKNRVPEGAITPRSKDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQA 120

Query: 121 YLNVKFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           YLNVKFKETGH+N YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 YLNVKFKETGHDNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHA+PEEAE
Sbjct: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHANPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMIDIYTKF+YEQAA+PV+ GRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDIYTKFAYEQAAMPVIPGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQF DENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI
Sbjct: 301 GQNFSRAFGTQFADENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360

Query: 361 IPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           IPIWKK+DD+AEVLSA+SSVK SLQTAGIKVKLD+S+QRTPGWKFNFWEMK
Sbjct: 361 IPIWKKDDDRAEVLSASSSVKESLQTAGIKVKLDDSDQRTPGWKFNFWEMK 411

[Close]

BLAST of Tan0020163.2 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 657.9 bits (1696), Expect = 5.4e-189
Identity = 322/405 (79.51%), Postives = 357/405 (88.15%), Query Frame = 0


Query: 4   SLRLPSLSSLLSPASPFSFSRRALGISWRQQRPFRLPLPASGFSVRSSSTGTEIRVDNSK 63
           SLRLPSL+SLL PA+    +     +  R +       P SGF+   S T +     +  
Sbjct: 5   SLRLPSLTSLLFPATTRYPATLRRTVCLRNR-------PLSGFATAPSGTASPETKSSEV 64

Query: 64  NRV-PEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQEYLNVKF 123
           +R+  + A+TPRSQDFNAWYLDVIA+AELADYGPVRGTMVIRPYGYAIWEAIQ+YLNVKF
Sbjct: 65  DRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKF 124

Query: 124 KETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNH 183
           KETGH N YFPQFIPYSFIEKEASHVEGFSPELALVT+GGGKELEEKLVVRPTSETIVNH
Sbjct: 125 KETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSETIVNH 184

Query: 184 MFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQM 243
           MFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLEFLWQEGHTAHA+PEEAEKEA QM
Sbjct: 185 MFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLEFLWQEGHTAHATPEEAEKEAKQM 244

Query: 244 IDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSR 303
           I+IYT+F++EQ AIPV+ GRKSK+ETFAGAD TYTIEAMMGDRKALQAGTSHNLGQNFSR
Sbjct: 245 IEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDRKALQAGTSHNLGQNFSR 304

Query: 304 AFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKK 363
           AFGTQF DENG+RQHVWQTSWA+STRFVGGIIMTHGDD GLMLPPK+APIQVVI+PIWKK
Sbjct: 305 AFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVPIWKK 364

Query: 364 EDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEMK 408
           + +K  VLSAASSVK +LQTAG++VKLD+++QRTPGWKFNFWEMK
Sbjct: 365 DTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMK 402

[Close]

BLAST of Tan0020163.2 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.6e-84
Identity = 150/361 (41.55%), Postives = 231/361 (63.99%), Query Frame = 0


Query: 49  RSSSTGTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGY 108
           ++SS+G + +  + K     G    + ++F  WY +V    ++ +Y  + G  ++RP+  
Sbjct: 31  KASSSGQKKK--DVKKETGLGLSVKKDENFGEWYSEV-CKQDMIEYYDISGCYILRPWSM 90

Query: 109 AIWEAIQEYLNVKFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEE 168
           AIWE +Q + + + K+   +N YFP F+    +EKE  H+EGF+PE+A VT  G  +LE 
Sbjct: 91  AIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEV 150

Query: 169 KLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFVRTLEFLWQEGHT 228
            + +RPTSET++   +++WI  +RDLPL +NQW NV RWE     PF+R+ EFLWQEGHT
Sbjct: 151 PIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHT 210

Query: 229 AHASPEEAEKEAIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDR-K 288
           A A+  EA++E +Q++++Y +   E  A+PVV G KS+ E FAG   T ++EA + +  +
Sbjct: 211 AFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGR 270

Query: 289 ALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLP 348
            +Q  TSH LGQNF++ F   F +E  + + VWQ SWA STR +G +IMTHGDD GL+LP
Sbjct: 271 GVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLP 330

Query: 349 PKLAPIQVVIIPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEM 408
           PK+A +QVV+IP+  K+ +   +  A ++   +L  AGI+ + D  +  +PGWK++ WEM
Sbjct: 331 PKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEM 388

[Close]

BLAST of Tan0020163.2 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.6e-84
Identity = 150/361 (41.55%), Postives = 231/361 (63.99%), Query Frame = 0


Query: 49  RSSSTGTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGY 108
           ++SS+G + +  + K     G    + ++F  WY +V    ++ +Y  + G  ++RP+  
Sbjct: 31  KASSSGQKKK--DVKKETGLGLSVKKDENFGEWYSEV-CKQDMIEYYDISGCYILRPWSM 90

Query: 109 AIWEAIQEYLNVKFKETGHENAYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEE 168
           AIWE +Q + + + K+   +N YFP F+    +EKE  H+EGF+PE+A VT  G  +LE 
Sbjct: 91  AIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEV 150

Query: 169 KLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFVRTLEFLWQEGHT 228
            + +RPTSET++   +++WI  +RDLPL +NQW NV RWE     PF+R+ EFLWQEGHT
Sbjct: 151 PIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHT 210

Query: 229 AHASPEEAEKEAIQMIDIYTKFSYEQAAIPVVAGRKSKVETFAGADKTYTIEAMMGDR-K 288
           A A+  EA++E +Q++++Y +   E  A+PVV G KS+ E FAG   T ++EA + +  +
Sbjct: 211 AFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGR 270

Query: 289 ALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLP 348
            +Q  TSH LGQNF++ F   F +E  + + VWQ SWA STR +G +IMTHGDD GL+LP
Sbjct: 271 GVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLP 330

Query: 349 PKLAPIQVVIIPIWKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESEQRTPGWKFNFWEM 408
           PK+A +QVV+IP+  K+ +   +  A ++   +L  AGI+ + D  +  +PGWK++ WEM
Sbjct: 331 PKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEM 388

[Close]

BLAST of Tan0020163.2 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 59.7 bits (143), Expect = 6.5e-09
Identity = 30/68 (44.12%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 335 MTHGDDGGLMLPPKLAPIQVVIIPI-WKKEDDKAEVLSAASSVKVSLQTAGIKVKLDESE 394
           MTHGDD GL+ PPK+AP+QVV+I +  K   D  E+  A  +V+ +L  AGI+ + D  +
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 395 QRTPGWKF 402
             + GWK+
Sbjct: 149 NYSCGWKY 156

The following BLAST results are available for this feature:

BLAST of Tan0020163.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYR6	7.5e-188	79.51	Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q72GF9	4.4e-127	62.36	Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / H...	[more]
Q5SM28	2.8e-126	62.07	Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)...	[more]
B3QPX3	6.7e-120	62.02	Proline--tRNA ligase OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=51...	[more]
Q3B2T0	8.8e-120	61.13	Proline--tRNA ligase OS=Pelodictyon luteolum (strain DSM 273 / 2530) OX=319225 G...	[more]

BLAST of Tan0020163.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023541480.1	6.7e-226	95.58	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo]	[more]
XP_022945172.1	2.6e-225	95.09	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata]	[more]
XP_038878181.1	1.8e-223	94.40	proline--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]	[more]
KAG7013253.1	3.1e-223	93.48	Proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp....	[more]
XP_022968367.1	4.1e-223	94.84	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima]	[more]

BLAST of Tan0020163.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G067	1.2e-225	95.09	Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111449493 PE=3 SV=1	[more]
A0A6J1HTC6	2.0e-223	94.84	Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111467631 PE=3 SV=1	[more]
A0A5D3CUH7	6.8e-216	91.99	Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A0A0L6I6	9.8e-215	91.06	Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G213510 PE=3 SV=1	[more]
A0A6J1C8A3	6.6e-211	89.78	Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1	[more]

BLAST of Tan0020163.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52520.1	5.4e-189	79.51	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.1	1.6e-84	41.55	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.2	1.6e-84	41.55	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	6.5e-09	44.12	tRNA synthetase-related / tRNA ligase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002316	Proline-tRNA ligase, class IIa	PRINTS	PR01046	TRNASYNTHPRO	coord: 200..208 score: 46.09 coord: 209..220 score: 34.26 coord: 129..147 score: 26.71 coord: 170..181 score: 41.67
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 66..345 e-value: 8.9E-106 score: 355.3
None	No IPR available	PANTHER	PTHR43382:SF7	BNAC09G28510D PROTEIN	coord: 2..408
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 342..412
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 77..340
IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	PFAM	PF00587	tRNA-synt_2b	coord: 165..334 e-value: 1.6E-20 score: 73.8
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 346..415 e-value: 2.7E-19 score: 70.8
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	TIGRFAM	TIGR00408	TIGR00408	coord: 72..407 e-value: 1.7E-138 score: 460.4
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	PANTHER	PTHR43382	PROLYL-TRNA SYNTHETASE	coord: 2..408
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 353..412 e-value: 1.7E-6 score: 28.1
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 98..346 score: 23.445723
IPR033721	Prolyl-tRNA synthetase, catalytic domain	CDD	cd00778	ProRS_core_arch_euk	coord: 77..336 e-value: 2.50802E-169 score: 472.851

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0020163	Tan0020163	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1884256..1884882	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1884986..1885069	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1885692..1885905	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1886464..1886625	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1887048..1887185	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1887453..1887659	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1887746..1887859	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1887956..1888042	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1888218..1888328	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1888414..1888470	exon
Tan0020163.2-exon	Tan0020163.2-exon-LG01:1888560..1889022	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0020163.2-three_prime_utr	Tan0020163.2-three_prime_utr-LG01:1884256..1884882	three_prime_UTR
Tan0020163.2-three_prime_utr	Tan0020163.2-three_prime_utr-LG01:1884986..1885069	three_prime_UTR
Tan0020163.2-three_prime_utr	Tan0020163.2-three_prime_utr-LG01:1885692..1885878	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1885879..1885905	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1886464..1886625	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1887048..1887185	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1887453..1887659	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1887746..1887859	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1887956..1888042	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1888218..1888328	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1888414..1888470	CDS
Tan0020163.2-cds	Tan0020163.2-cds-LG01:1888560..1888904	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0020163.2-five_prime_utr	Tan0020163.2-five_prime_utr-LG01:1888905..1889022	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0020163.2	Tan0020163.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006433	prolyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0009553	embryo sac development
biological_process	GO:0048481	plant ovule development
biological_process	GO:0010109	regulation of photosynthesis
biological_process	GO:0048316	seed development
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0004827	proline-tRNA ligase activity