Tan0011467.2 (mRNA) Snake gourd v1

Overview


Name	Tan0011467.2
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	3-methyl-2-oxobutanoate hydroxymethyltransferase
Location	LG06: 15339199 .. 15342204 (+)
Sequence length	1356
RNA-Seq Expression	Tan0011467.2
Synteny	Tan0011467.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCGTGAAAAGGTATATTCTATCCAATTTTCTCAAGAATTTGACACCTACCGCCGCCGGCGGAATTCCACCTGCTAATTCGCGGCAACAGTGCAACATATTGTGAAGTTCCATTACAATTCTCTCGAAACCCCTATTTTCCTCCATCAATGACGATCTTCAACTTCTTCGCCATGGCTGCAGCTCGAACCAGAACGCGATCACTGCTCAAAACGCTTCGTAACTTTAGCAATGTTCCTGAAAACACAGTCTATGGCGGCCCCACGACGCAGAATCCAAACAAGAGAGTCACTCTCACCCATTTGAGACAAAAGCACAAGAAAGGCGAACCGATCACCGTGGTCACTGCTTACGATTACCCCTCCGGAGTCCATGTGGACATGGCCGGCATTGATATTGCTTTGGTCGGTGATTCGGCTTCCATGGTAGTGCACGGCCACGATACCACTTTGCCTATTAGCTTGGATGAAATGCTTGTTCATTGCAGGGCTGTTGCTCGTGGGGCTAGGAGGCCACTTTTGGTCGGCGATTTGCCCTTCGGTACCTATGAATCCAGCTCCGTACAGGTTGTTTGCCTTTTTCCCTTTTAGGTGTTTGAAGTATTTGTGTAAAGTTAGAGAATTTTGTCTTTGTTGTTGTTTGAGATGGACCGCACGGGAATCTAAGGAATTCATTTGTTTTCAAAAGTAAAAGCAATTTCGATTGTCAAAAAAAAAAAAAAAAAAGAAAAGCAATTCCCTGCTCTTGTAAGAATGTCCACTTTTTCTTGCTGGTGGGGTTATCTAAACTATTGAACAGGGGAGGTCTTAAATTCATATCAAAGTATGTTTTCATTTAGTTTCTTAGTTAAATTAGGCCAACATTATTTCTCAAAATGCATAAACAGACCAATTTTGTGGAACAATTTATTTTGGTAACTCAGAAGAAAGCTAGTTTAGAAATGTAAGAACTTATCTTTGATAGGAAACTTGAGTGGGATTGGGTGGCCTTAATTGAATTGTAGGAGCAGCCTTCTTTTATAAGGTGCCAAAACCTTAAACTAGGTCAACTTAGTGCAGAAACTTCAACAAATAGATTCCCACTTGACGAAAGTATTGTACACAACACCTCGATTCTTAAAATGATTCAGAAATGGGTTTCTATTATTGACAGGCTGTTGATACGGCAGTAAGGATTCTGAAGGAAGGCAGAATGGATGCTATAAAATTGGAAGGGGGCGCGCAGTCTAGAATAACAGCAGCAAAAGCTATCGTCGACGCAGGAATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGAATGAATCTTGCCAGTGCTCTTAAGGTACCCCCATTCCCTTGTCTCTTCTTCCAGGCACCATTTTTGTTGTGATGTTGCATAGTTTGTCATGTTCATTTCAGCTGTTATGCTGAATGGCGTATCTTTGTAGGTCGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTTCTGTCGTTCTAGAATGTGTTCCTCCACAAGTTGCAGCTGCAGCTACAGCTGCAGTCCGTATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTTAGTTGATTGATTGCATAGTCATTGCCACACACACATGTTTATATTTGACTGCAAATGTCTTTCTTTCAGCAGTTTGATAGTTTTTCTTTGTTAGATCTGACATGTATTCTACATTTAAACTATTTCTTTACACATTAGTAGAGGAATCAGAGTTTTTCTCCCGAGTTCCAAATGAGAGAGAAGCATCGAGGTTGGCCTTGTTTAGTCTTCTTAAATGGTCTTTTTCTATGGTTCTTCAACTTCTGCATACTGGAAGTGAGAATCCATATTCAAAGTCGATGCATCAGAAGTTAGAAGAAATATTAAAGACTGATAAAATACTTCCACTGGCAAACGGTCAACCTGATAAGATTTCATGGAGGCAAAGTTAGCAATTTGTCAATATTATGAGTTTGGATGCTGCTCTCTCGAATTTGGAACGAGCTTGAGGAGGAAAAAACAGACCCTTAAATCTCACTCAACTGTATGATTTATTCAATATTTTAAAATGCAACTTATTGGTTTTTTATTTTTTGTTTTTCTTTCAACAGAAATGACCATAGATATGTTGGTTTCATCATCCATACTTGTGTGATAAGGATCATGAACATAATAATTGTACACTACTACGATGAAATTCCTGCAAACATCATTGGCCATCATGTTTAGGTTTAATTTGTGCAGTTAAACTTCACATGGAACTTCAGTTCTCTCTATTGACAATGGTTTTGTATTGACGGTATATTTCATTTCACTAATCCGTTCATTATTGTCCATAGTCTTGTTGCTCTTCTTTTCTTTGATCACTTGGCAACAATCATAGTGTCGACCGTGTCGAATATCCTCTGCAGCTGAACTAACTAATTTCTTGCACTCAGGTGCTAGTTTTCCACGATCTGCTCGGCATGCTTCAACACCCGCATCATGCTAAGGTTATCATCAGGCCACACCTTTGTTTCGACTAAGTCTCTCTATACTTGTGATTGTTTACGATGTTCGATTCCTGGATTGTATATACCCTTTTTTGCAGGTGACCCCGAAATTCTGTAAGCAATTTGCACACGTTGGAAATGTCATCAACAAGGCTTTATCAGATTACAAGGAAGAAGTATCAAACGGTTCGTTTCCCGGTCCTCTCCATAGCCCATATAAGATCAGCAATGACGAATTCAATGGTTTCTTGAAAGAGTTACAGAATATGGGATTGGATGAGGCAGCTTCTGCAGCTGCTGCTAAAGCCAACCAGTGATTGAACTCAGGCAACATTTAGTGGTCGCCCTCTTTGGCCTTGCTTCCTTTTTGAGTTTCAAATAATTCATGGAATTTATAAGTTAGGACAATTTTCATTCAAACAATCGTACTTGATAAACACTTGTGCATAAAGATTGCAATCAACGTTCTCTTTGGGCGGAC

mRNA sequence

CGCGTGAAAAGGTATATTCTATCCAATTTTCTCAAGAATTTGACACCTACCGCCGCCGGCGGAATTCCACCTGCTAATTCGCGGCAACAGTGCAACATATTGTGAAGTTCCATTACAATTCTCTCGAAACCCCTATTTTCCTCCATCAATGACGATCTTCAACTTCTTCGCCATGGCTGCAGCTCGAACCAGAACGCGATCACTGCTCAAAACGCTTCGTAACTTTAGCAATGTTCCTGAAAACACAGTCTATGGCGGCCCCACGACGCAGAATCCAAACAAGAGAGTCACTCTCACCCATTTGAGACAAAAGCACAAGAAAGGCGAACCGATCACCGTGGTCACTGCTTACGATTACCCCTCCGGAGTCCATGTGGACATGGCCGGCATTGATATTGCTTTGGTCGGTGATTCGGCTTCCATGGTAGTGCACGGCCACGATACCACTTTGCCTATTAGCTTGGATGAAATGCTTGTTCATTGCAGGGCTGTTGCTCGTGGGGCTAGGAGGCCACTTTTGGTCGGCGATTTGCCCTTCGGTACCTATGAATCCAGCTCCGTACAGGCTGTTGATACGGCAGTAAGGATTCTGAAGGAAGGCAGAATGGATGCTATAAAATTGGAAGGGGGCGCGCAGTCTAGAATAACAGCAGCAAAAGCTATCGTCGACGCAGGAATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGAATGAATCTTGCCAGTGCTCTTAAGGTCGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTTCTGTCGTTCTAGAATGTGTTCCTCCACAAGTTGCAGCTGCAGCTACAGCTGCAGTCCGTATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTTCCACGATCTGCTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCACACGTTGGAAATGTCATCAACAAGGCTTTATCAGATTACAAGGAAGAAGTATCAAACGGTTCGTTTCCCGGTCCTCTCCATAGCCCATATAAGATCAGCAATGACGAATTCAATGGTTTCTTGAAAGAGTTACAGAATATGGGATTGGATGAGGCAGCTTCTGCAGCTGCTGCTAAAGCCAACCAGTGATTGAACTCAGGCAACATTTAGTGGTCGCCCTCTTTGGCCTTGCTTCCTTTTTGAGTTTCAAATAATTCATGGAATTTATAAGTTAGGACAATTTTCATTCAAACAATCGTACTTGATAAACACTTGTGCATAAAGATTGCAATCAACGTTCTCTTTGGGCGGAC

Coding sequence (CDS)

ATGACGATCTTCAACTTCTTCGCCATGGCTGCAGCTCGAACCAGAACGCGATCACTGCTCAAAACGCTTCGTAACTTTAGCAATGTTCCTGAAAACACAGTCTATGGCGGCCCCACGACGCAGAATCCAAACAAGAGAGTCACTCTCACCCATTTGAGACAAAAGCACAAGAAAGGCGAACCGATCACCGTGGTCACTGCTTACGATTACCCCTCCGGAGTCCATGTGGACATGGCCGGCATTGATATTGCTTTGGTCGGTGATTCGGCTTCCATGGTAGTGCACGGCCACGATACCACTTTGCCTATTAGCTTGGATGAAATGCTTGTTCATTGCAGGGCTGTTGCTCGTGGGGCTAGGAGGCCACTTTTGGTCGGCGATTTGCCCTTCGGTACCTATGAATCCAGCTCCGTACAGGCTGTTGATACGGCAGTAAGGATTCTGAAGGAAGGCAGAATGGATGCTATAAAATTGGAAGGGGGCGCGCAGTCTAGAATAACAGCAGCAAAAGCTATCGTCGACGCAGGAATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGAATGAATCTTGCCAGTGCTCTTAAGGTCGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTTCTGTCGTTCTAGAATGTGTTCCTCCACAAGTTGCAGCTGCAGCTACAGCTGCAGTCCGTATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTTCCACGATCTGCTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCACACGTTGGAAATGTCATCAACAAGGCTTTATCAGATTACAAGGAAGAAGTATCAAACGGTTCGTTTCCCGGTCCTCTCCATAGCCCATATAAGATCAGCAATGACGAATTCAATGGTTTCTTGAAAGAGTTACAGAATATGGGATTGGATGAGGCAGCTTCTGCAGCTGCTGCTAAAGCCAACCAGTGA

Protein sequence

MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGEPITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ

Homology

[Close]

BLAST of Tan0011467.2 vs. ExPASy Swiss-Prot
Match: O82357 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 8.6e-153
Identity = 274/340 (80.59%), Postives = 305/340 (89.71%), Query Frame = 0


Query: 9   MAAARTRTRSLLK---TLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGEPITVV 68
           MA++ TR  S      ++R  SN+PENTVYGGP  QNPN+RVTLTHLRQKH++GEPITVV
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 69  TAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLV 128
           TAYDYPS VH+D AGID+ LVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGA+RPLLV
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 129 GDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGL 188
           GDLPFGTYESSS QAVDTAVR+LKEG MDAIKLEGG+ SRITAAKAIV+AGIAV+GHVGL
Sbjct: 121 GDLPFGTYESSSSQAVDTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGL 180

Query: 189 TPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPT 248
           TPQAISVLGGFRPQG N+ASA+KVVETA++LQEAGCFSVVLECVPP VAAAAT+A++IPT
Sbjct: 181 TPQAISVLGGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPT 240

Query: 249 IGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNG 308
           IGIGAGPFCSGQVLV+HDLLGM+QHPHHAKVTPKFCKQ+A+VG VINKAL +YKEEVS  
Sbjct: 241 IGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKK 300

Query: 309 SFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKA 346
            FPGP HSPYKI+  E +GFL ELQ +G D+AASAAA  A
Sbjct: 301 VFPGPSHSPYKITASELDGFLTELQKLGFDKAASAAALAA 340

[Close]

BLAST of Tan0011467.2 vs. ExPASy Swiss-Prot
Match: Q9M315 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.0e-150
Identity = 266/334 (79.64%), Postives = 300/334 (89.82%), Query Frame = 0


Query: 14  TRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGEPITVVTAYDYPSG 73
           +R    + T+R  SNVPE+TVYGGP  QN N+RVTLT LRQKH+KGEPIT+VTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LKEG MDAIKLEGG+ SRITAAK+IV+AGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPTIGIGAGPF 253
           GGFRPQG N+ASA+KVVETA++LQEAGCFSVVLECVPP VAAAAT+A+ IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLV+HDLLGM+QHPHHAKVTPKFCKQ+A VG VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           PYKIS+ + +GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]

BLAST of Tan0011467.2 vs. ExPASy Swiss-Prot
Match: Q9AWZ8 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.1e-138
Identity = 253/334 (75.75%), Postives = 285/334 (85.33%), Query Frame = 0


Query: 24  RNFSNVPENTVYGGPTTQN------------PNKRVTLTHLRQKHKKGEPITVVTAYDYP 83
           R  S+VPE+ VYGGP  Q+              +RVT+T LR KH++GEPITVVTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  SGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFG 143
           S VHVD AGID+ LVGDSA+MVVHGHDTTLPISLD ML HCRAVARGA RPLLVGDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGLTPQAIS 203
            YESSS +AVD+AVR+LKEG MDAIKLEGGA SRI+AAKAIV+AGIAVMGHVGLTPQAIS
Sbjct: 138 CYESSSTRAVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAIS 197

Query: 204 VLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPTIGIGAG 263
           VLGGFRPQG  + SA+KVVETA++LQEAGCFSVVLECVP  VAAAAT+A++IPTIGIGAG
Sbjct: 198 VLGGFRPQGKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAG 257

Query: 264 PFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNGSFPGPL 323
           PFCSGQVLV+HDLLGM+QHPHHAKVTPKFCKQF +VG+VINKALS+YK+EV   SFPGP 
Sbjct: 258 PFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSFPGPS 317

Query: 324 HSPYKISNDEFNGFLKELQNMGLDEAASAAAAKA 346
           H+PYKI+  + +GF   LQ MGLDEAA+AAAA A
Sbjct: 318 HTPYKIAAADVDGFANALQKMGLDEAANAAAAAA 351

[Close]

BLAST of Tan0011467.2 vs. ExPASy Swiss-Prot
Match: Q9AWZ7 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.1e-115
Identity = 212/318 (66.67%), Postives = 257/318 (80.82%), Query Frame = 0


Query: 31  ENTVYGG------PTTQNPNKRVTLTHLRQKHKKGEPITVVTAYDYPSGVHVDMAGIDIA 90
           E  +YGG        +    +RVTL  LR KH++GEPI++VTAYDYPSGVHVD AG DI 
Sbjct: 62  EPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHVDAAGFDIC 121

Query: 91  LVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVDTA 150
           LVGDSA+MV HGHD TLPISLD M+ HCRAVARGA R  LVGDLPFG+YE+S+ QAV +A
Sbjct: 122 LVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEASTAQAVGSA 181

Query: 151 VRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGLTPQAISVLGGFRPQGMNLA 210
           VR++KEG +++IKLEG A SRI+AA+AIVDAGIAVMGH+GLTPQ++S LGGFRPQG  + 
Sbjct: 182 VRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGFRPQGKTVE 241

Query: 211 SALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPTIGIGAGPFCSGQVLVFHDL 270
           SA+KVVE A++LQEAGCF+VVLECVP  VAAAAT+A+ IPTIGIGAGPFCSGQVLV+HDL
Sbjct: 242 SAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSGQVLVYHDL 301

Query: 271 LGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNGSFPGPLHSPYKISNDEFNG 330
           LG  Q   HAKV+PKFCKQ+ ++G+VIN+ALS YK+EV   SFPGP H+PYK++  + + 
Sbjct: 302 LGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSHTPYKLAATDVDA 361

Query: 331 FLKELQNMGLDEAASAAA 343
           FL  L+  GL+ AA AAA
Sbjct: 362 FLNALKMKGLNVAADAAA 378

[Close]

BLAST of Tan0011467.2 vs. ExPASy Swiss-Prot
Match: B0TC10 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=panB PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 7.0e-70
Identity = 135/269 (50.19%), Postives = 189/269 (70.26%), Query Frame = 0


Query: 45  KRVTLTHLRQKHKKGEPITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPIS 104
           K++TL   ++  K+G+ + ++TAYDYP    VD + I+I LVGDS  MVV G+D+T+P++
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQS 164
           LDEM+ HC+ V RGA   L+V D+PFG+Y  S   A+  A R+LKE  ++A+K+EGG + 
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSYNVSKEDAIRNANRMLKESGIEAVKVEGGTRM 122

Query: 165 RITAAKAIVDAGIAVMGHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSV 224
             T  +A+VDAGI VMGH+GLTPQ  + LGGF+ QG    +A +++E A++L+ AG FS+
Sbjct: 123 APT-VRALVDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSI 182

Query: 225 VLECVPPQVAAAATAAVRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQF 284
           V+ECVP  +A   TA++ IPTIGIGAGP+C+GQVLV  DLLG+     + +  PKF KQ+
Sbjct: 183 VIECVPAGLARTITASLSIPTIGIGAGPYCNGQVLVIQDLLGI-----YDRFVPKFVKQY 242

Query: 285 AHVGNVINKALSDYKEEVSNGSFPGPLHS 314
           A  G  I  AL+DY  EV++G FPGP HS
Sbjct: 243 AQTGPAIRAALNDYAREVADGVFPGPEHS 265

[Close]

BLAST of Tan0011467.2 vs. NCBI nr
Match: XP_038898896.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 4.0e-171
Identity = 307/347 (88.47%), Postives = 334/347 (96.25%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M IFN F+ AA+RTRTR LLKTLRN SNVPEN VYGGPT+Q PN RVTLT+LRQK+KKGE
Sbjct: 1   MAIFNLFSKAASRTRTRPLLKTLRNMSNVPENAVYGGPTSQTPNHRVTLTNLRQKYKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSA+MVVHG+DTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGYDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVR+LKEG MDAIKLEGGA SRITAA+AIV+AGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ASA+KVVETAISLQEAGCF+VVLECVPP+VAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATSA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPT+GIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKALS+Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKIS+ +FNGFLKELQNMGLDEAASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDTDFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of Tan0011467.2 vs. NCBI nr
Match: XP_022147525.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial [Momordica charantia])

HSP 1 Score: 605.5 bits (1560), Expect = 2.8e-169
Identity = 306/343 (89.21%), Postives = 327/343 (95.34%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +FNFF +A  RTRTRSLLKTLR  SNVPENTVYGGP  Q P +RVTLT+LRQKHKKGE
Sbjct: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYP+GVH+DMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVRILKEG MDAIKLEGGA SRITAAKAIV+AGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ASA+KVVETAI+LQEAGCF+VVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPT+GIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA VG+VIN+AL DYKE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKISND++NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 343

[Close]

BLAST of Tan0011467.2 vs. NCBI nr
Match: XP_004139651.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KGN44587.1 hypothetical protein Csa_016545 [Cucumis sativus])

HSP 1 Score: 600.1 bits (1546), Expect = 1.2e-167
Identity = 299/347 (86.17%), Postives = 330/347 (95.10%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +F    MA +RTRT+SLLKTLR  SNVPENTVYGGPT+Q PN+RVTLT+LRQK+KKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLL+GDLPFGTYESSS QA+DTAVRILKEG MDAIKLEGG+ SRITAA+AIV+AGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ SA+KVVETAISLQEAGCF+VVLECVPP+VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPT+GIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKIS+ +FNGFLKELQNMGLDEAASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of Tan0011467.2 vs. NCBI nr
Match: XP_022991968.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 599.0 bits (1543), Expect = 2.7e-167
Identity = 300/347 (86.46%), Postives = 330/347 (95.10%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +FN    AA R+ ++SLL TLR  SNVPENTVYGGPT+  PN+RVTLT+L+QK+KKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEG MDAIKLEGGA SRITAA+AIV+AGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ASA+KVVETAISLQEAGCF+VVLECVPP+VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPTIGIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKIS++++NGFLKEL++MGLD AASAAAAKANQ
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

[Close]

BLAST of Tan0011467.2 vs. NCBI nr
Match: KAG6575974.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 597.8 bits (1540), Expect = 5.9e-167
Identity = 299/347 (86.17%), Postives = 330/347 (95.10%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +FN    AA R+ ++SLL TLR  SNVPENTVYGGPT+  PN+RVTLT+L+QK+KKGE
Sbjct: 117 MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 176

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 177 PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 236

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEG MDAIKLEGGA SRITAA+AIV+AGIAVM
Sbjct: 237 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 296

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ASA+KVVETAISLQEAGCF+VVLECVPP+VAAAATAA
Sbjct: 297 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 356

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPTIGIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKALSDYKE
Sbjct: 357 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 416

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKIS+D++NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 417 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 463

[Close]

BLAST of Tan0011467.2 vs. ExPASy TrEMBL
Match: A0A6J1D0E3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111016428 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.4e-169
Identity = 306/343 (89.21%), Postives = 327/343 (95.34%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +FNFF +A  RTRTRSLLKTLR  SNVPENTVYGGP  Q P +RVTLT+LRQKHKKGE
Sbjct: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYP+GVH+DMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVRILKEG MDAIKLEGGA SRITAAKAIV+AGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ASA+KVVETAI+LQEAGCF+VVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPT+GIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA VG+VIN+AL DYKE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKISND++NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 343

[Close]

BLAST of Tan0011467.2 vs. ExPASy TrEMBL
Match: A0A0A0K8B2 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.8e-168
Identity = 299/347 (86.17%), Postives = 330/347 (95.10%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +F    MA +RTRT+SLLKTLR  SNVPENTVYGGPT+Q PN+RVTLT+LRQK+KKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLL+GDLPFGTYESSS QA+DTAVRILKEG MDAIKLEGG+ SRITAA+AIV+AGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ SA+KVVETAISLQEAGCF+VVLECVPP+VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPT+GIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKIS+ +FNGFLKELQNMGLDEAASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of Tan0011467.2 vs. ExPASy TrEMBL
Match: A0A6J1JXR6 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488459 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 300/347 (86.46%), Postives = 330/347 (95.10%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +FN    AA R+ ++SLL TLR  SNVPENTVYGGPT+  PN+RVTLT+L+QK+KKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEG MDAIKLEGGA SRITAA+AIV+AGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ASA+KVVETAISLQEAGCF+VVLECVPP+VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPTIGIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKIS++++NGFLKEL++MGLD AASAAAAKANQ
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

[Close]

BLAST of Tan0011467.2 vs. ExPASy TrEMBL
Match: A0A6J1GNW3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456114 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.9e-167
Identity = 299/347 (86.17%), Postives = 330/347 (95.10%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +FN    AA R+ ++SLL TLR  SNVPENTVYGGPT+  PN+RVTLT+L+QK+KKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEG MDAIKLEGGA SRITAA+AIV+AGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ASA+KVVETAISLQEAGCF+VVLECVPP+VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPTIGIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKIS+D++NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]

BLAST of Tan0011467.2 vs. ExPASy TrEMBL
Match: A0A1S3BH28 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103489751 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.6e-165
Identity = 298/347 (85.88%), Postives = 326/347 (93.95%), Query Frame = 0


Query: 1   MTIFNFFAMAAARTRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGE 60
           M +F    MAA+RTR +SLLKTLR  SNVPENTVY GPT+Q PN+RVTLT+LRQK+KKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITVVTAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PIT+VTAYDYPSGVH+DMAGIDIALVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVM 180
           RPLL+GDLPFGTYESSS QAVDTAVRILKEG MDAIKLEGGA SRITAA+AIV+AGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG N+ SA+KVVETAISLQEAGCF+VVLECVPP+VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 VRIPTIGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKE 300
           +RIPT+GIGAGPFCSGQVLV+HDLLGMLQHPHHAKVTPKFCKQFA +G+VINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           EVSN SFPGP HSPYKIS+ + NGFLKELQNMGLDEAASAAAAKAN+
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of Tan0011467.2 vs. TAIR 10
Match: AT2G46110.1 (ketopantoate hydroxymethyltransferase 1 )

HSP 1 Score: 541.2 bits (1393), Expect = 6.1e-154
Identity = 274/340 (80.59%), Postives = 305/340 (89.71%), Query Frame = 0


Query: 9   MAAARTRTRSLLK---TLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGEPITVV 68
           MA++ TR  S      ++R  SN+PENTVYGGP  QNPN+RVTLTHLRQKH++GEPITVV
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 69  TAYDYPSGVHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLV 128
           TAYDYPS VH+D AGID+ LVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGA+RPLLV
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 129 GDLPFGTYESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGL 188
           GDLPFGTYESSS QAVDTAVR+LKEG MDAIKLEGG+ SRITAAKAIV+AGIAV+GHVGL
Sbjct: 121 GDLPFGTYESSSSQAVDTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGL 180

Query: 189 TPQAISVLGGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPT 248
           TPQAISVLGGFRPQG N+ASA+KVVETA++LQEAGCFSVVLECVPP VAAAAT+A++IPT
Sbjct: 181 TPQAISVLGGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPT 240

Query: 249 IGIGAGPFCSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNG 308
           IGIGAGPFCSGQVLV+HDLLGM+QHPHHAKVTPKFCKQ+A+VG VINKAL +YKEEVS  
Sbjct: 241 IGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKK 300

Query: 309 SFPGPLHSPYKISNDEFNGFLKELQNMGLDEAASAAAAKA 346
            FPGP HSPYKI+  E +GFL ELQ +G D+AASAAA  A
Sbjct: 301 VFPGPSHSPYKITASELDGFLTELQKLGFDKAASAAALAA 340

[Close]

BLAST of Tan0011467.2 vs. TAIR 10
Match: AT3G61530.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-151
Identity = 266/334 (79.64%), Postives = 300/334 (89.82%), Query Frame = 0


Query: 14  TRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGEPITVVTAYDYPSG 73
           +R    + T+R  SNVPE+TVYGGP  QN N+RVTLT LRQKH+KGEPIT+VTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LKEG MDAIKLEGG+ SRITAAK+IV+AGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPTIGIGAGPF 253
           GGFRPQG N+ASA+KVVETA++LQEAGCFSVVLECVPP VAAAAT+A+ IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLV+HDLLGM+QHPHHAKVTPKFCKQ+A VG VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           PYKIS+ + +GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]

BLAST of Tan0011467.2 vs. TAIR 10
Match: AT3G61530.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-151
Identity = 266/334 (79.64%), Postives = 300/334 (89.82%), Query Frame = 0


Query: 14  TRTRSLLKTLRNFSNVPENTVYGGPTTQNPNKRVTLTHLRQKHKKGEPITVVTAYDYPSG 73
           +R    + T+R  SNVPE+TVYGGP  QN N+RVTLT LRQKH+KGEPIT+VTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHVDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKEGRMDAIKLEGGAQSRITAAKAIVDAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LKEG MDAIKLEGG+ SRITAAK+IV+AGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGMNLASALKVVETAISLQEAGCFSVVLECVPPQVAAAATAAVRIPTIGIGAGPF 253
           GGFRPQG N+ASA+KVVETA++LQEAGCFSVVLECVPP VAAAAT+A+ IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVFHDLLGMLQHPHHAKVTPKFCKQFAHVGNVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLV+HDLLGM+QHPHHAKVTPKFCKQ+A VG VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISNDEFNGFLKELQNMGLDEAASAAAAKANQ 348
           PYKIS+ + +GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

The following BLAST results are available for this feature:

BLAST of Tan0011467.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82357	8.6e-153	80.59	3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis...	[more]
Q9M315	4.0e-150	79.64	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis...	[more]
Q9AWZ8	2.1e-138	75.75	3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ...	[more]
Q9AWZ7	1.1e-115	66.67	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ...	[more]
B0TC10	7.0e-70	50.19	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum...	[more]

BLAST of Tan0011467.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898896.1	4.0e-171	88.47	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincas...	[more]
XP_022147525.1	2.8e-169	89.21	3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial [Momordica cha...	[more]
XP_004139651.1	1.2e-167	86.17	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativ...	[more]
XP_022991968.1	2.7e-167	86.46	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit...	[more]
KAG6575974.1	5.9e-167	86.17	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucu...	[more]

BLAST of Tan0011467.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D0E3	1.4e-169	89.21	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 ...	[more]
A0A0A0K8B2	5.8e-168	86.17	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1JXR6	1.3e-167	86.46	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1GNW3	2.9e-167	86.17	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 G...	[more]
A0A1S3BH28	1.6e-165	85.88	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Tan0011467.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46110.1	6.1e-154	80.59	ketopantoate hydroxymethyltransferase 1	[more]
AT3G61530.1	2.8e-151	79.64	Phosphoenolpyruvate carboxylase family protein	[more]
AT3G61530.2	2.8e-151	79.64	Phosphoenolpyruvate carboxylase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 44..326 e-value: 3.1E-118 score: 396.0
IPR003700	Ketopantoate hydroxymethyltransferase	TIGRFAM	TIGR00222	TIGR00222	coord: 46..313 e-value: 3.7E-84 score: 280.3
IPR003700	Ketopantoate hydroxymethyltransferase	PFAM	PF02548	Pantoate_transf	coord: 46..309 e-value: 2.9E-105 score: 351.3
IPR003700	Ketopantoate hydroxymethyltransferase	PANTHER	PTHR20881	3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE	coord: 13..346
IPR003700	Ketopantoate hydroxymethyltransferase	HAMAP	MF_00156	PanB	coord: 47..314 score: 39.269592
IPR003700	Ketopantoate hydroxymethyltransferase	CDD	cd06557	KPHMT-like	coord: 49..308 e-value: 6.28208E-141 score: 397.56
None	No IPR available	PIRSR	PIRSR000388-1	PIRSR000388-1	coord: 55..309 e-value: 3.1E-88 score: 293.1
None	No IPR available	PANTHER	PTHR20881:SF1	3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE	coord: 13..346
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 46..312

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0011467	Tan0011467	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0011467.2-five_prime_utr	Tan0011467.2-five_prime_utr-LG06:15339199..15339346	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0011467.2-exon	Tan0011467.2-exon-LG06:15339199..15339763	exon
Tan0011467.2-exon	Tan0011467.2-exon-LG06:15340350..15340556	exon
Tan0011467.2-exon	Tan0011467.2-exon-LG06:15340663..15340809	exon
Tan0011467.2-exon	Tan0011467.2-exon-LG06:15341670..15341723	exon
Tan0011467.2-exon	Tan0011467.2-exon-LG06:15341822..15342204	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0011467.2-cds	Tan0011467.2-cds-LG06:15339347..15339763	CDS
Tan0011467.2-cds	Tan0011467.2-cds-LG06:15340350..15340556	CDS
Tan0011467.2-cds	Tan0011467.2-cds-LG06:15340663..15340809	CDS
Tan0011467.2-cds	Tan0011467.2-cds-LG06:15341670..15341723	CDS
Tan0011467.2-cds	Tan0011467.2-cds-LG06:15341822..15342040	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0011467.2-three_prime_utr	Tan0011467.2-three_prime_utr-LG06:15342041..15342204	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0011467.2	Tan0011467.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015940	pantothenate biosynthetic process
biological_process	GO:0032259	methylation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0003864	3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0008168	methyltransferase activity