Tan0010204.1 (mRNA) Snake gourd v1

BLAST of Tan0010204.1 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 7.4e-197
Identity = 382/721 (52.98%), Postives = 513/721 (71.15%), Query Frame = 0

Query: 44  RSFRCTIPQSVVG----SSPSHEISRM--SPLKPPKIPVESAV---RRPSLARSSFSKSK 103
           RS     P+S  G     SPS EIS++  SP KPP+ P ++ V   +R S ARS +SK K
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPDGLSLAEEKTQAKSTSGSAFYSSPKMDSPAKITTATSPREALKSAPITPKT 163
           SR V+P CP   S+ EE+ + +  +G +F  +    SP       S R     AP+TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRA----SP----NNKSNRSVGSPAPVTPSK 147

Query: 164 PLIGSTGSEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLV 223
            ++     E+++DEE+Y+  +L  + RS  K+    LIE   F+ +   L+ASLT+  L 
Sbjct: 148 VVV-----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLK 207

Query: 224 TKEIWGLGMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRKSVII 283
               WGL +WKWCVLV+VIF G L + WF+ ++VFLIE NFLL+RKVLYFV+GL+KSV +
Sbjct: 208 HHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQV 267

Query: 284 FIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAALWLVKTLLVKILAASF 343
           FIWL L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F
Sbjct: 268 FIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANF 327

Query: 344 QCARFFDRIQESIFHQYILRTLSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKE 403
               FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  STG LSF  + K+   G   ++
Sbjct: 328 NVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEK 387

Query: 404 EVIDVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEEVEQKDKEINSEW 463
           +VID+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE 
Sbjct: 388 KVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEM 447

Query: 464 EARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWL 523
           EA AAAY +FRNVA+P   YI+EEDL RFM KEEVD V PLF+GA ETG+I RK+   W+
Sbjct: 448 EALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWV 507

Query: 524 VNVYVERKSLAHSLNDTKTAIEELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLL 583
           V VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+QL+
Sbjct: 508 VKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLV 567

Query: 584 LVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIF 643
            + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++
Sbjct: 568 ALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVY 627

Query: 644 YPNSVLATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHS 703
           YPN+VLATKPISN++RSP M +++EFS+ FST +  I  LK RI  Y+E  PQ W P HS
Sbjct: 628 YPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHS 687

Query: 704 ILVKEIENVNKMKMGLYVNHTINFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQ 755
           ++VKEIEN+NK+KM LY +HTI FQ   +++ RR+EL L +K++ EDL I Y LLPQ++ 
Sbjct: 688 VVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDIN 730

BLAST of Tan0010204.1 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 9.4e-176
Identity = 354/759 (46.64%), Postives = 499/759 (65.74%), Query Frame = 0

Query: 8   EQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPSHEISRMS 67
           E+VV+ + D E + D  +  P  +  + PD +    +S +   P S+    P+ EI + S
Sbjct: 10  EEVVINVSDKEDSKD-PRASPSFNPLASPDSDAGIEKS-KPVPPISI----PTPEIYKFS 69

Query: 68  PL--KPPKIP-VESAVRRPSLARSSFSKSKSRLVEPPC---------PDGLSLAEEKTQA 127
               KPPKIP  E  VRR SL+RS +SK KSR  E              G SL E+    
Sbjct: 70  GSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAG 129

Query: 128 KSTSGSAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAE 187
               GS   +SP   S   + +A   + A                  E +++EE+Y+  +
Sbjct: 130 SFARGSFDRASPNNKSNRSVASAALSKVA----------------EEEPDENEEIYKKVK 189

Query: 188 LKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFC 247
           L   +RSG  +K    +E V F+ + G LI SLT++ +    IWGL  WKWCVLV+V   
Sbjct: 190 LHRVKRSG--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLS 249

Query: 248 GRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKR 307
           G L + WF++ +VF+IE+N+LL++KVLYFV+GL+K+V +FIW +LVL+AW  LFD   KR
Sbjct: 250 GMLVTNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKR 309

Query: 308 SKKGNEILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRT 367
           ++K    L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+T
Sbjct: 310 TRKTKRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQT 369

Query: 368 LSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTM 427
           LSGPPL+E AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTM
Sbjct: 370 LSGPPLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTM 429

Query: 428 RGLINVIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYID 487
           R LI  + +SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+
Sbjct: 430 RVLIEAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIE 489

Query: 488 EEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIE 547
           E+DL RFM KEEVD VLPL E A +TGKI RK+   W+VNVY  RK++ HSLNDTKTA++
Sbjct: 490 EDDLLRFMIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVK 549

Query: 548 ELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFV 607
           +L+KL + ++ ++  I W++L+   +T++L+  SSQ L + FM G+T + +FE+ +FVFV
Sbjct: 550 QLDKLITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFV 609

Query: 608 MHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSD 667
           MHP+DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+M D
Sbjct: 610 MHPYDVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGD 669

Query: 668 SIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTI 727
            ++F + FST  E IG LK +I  Y+ +  Q W P   ++V+ IEN+NK+ + + V HTI
Sbjct: 670 YVDFGIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTI 729

Query: 728 NFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQL 755
           NFQ Y +KS RR+ L++ +K+I EDL I Y LLPQ+V L
Sbjct: 730 NFQVYVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of Tan0010204.1 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 6.8e-150
Identity = 303/718 (42.20%), Postives = 453/718 (63.09%), Query Frame = 0

Query: 81  RRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSGSAFYSSPKMDSPAKITTATS 140
           ++P L+R   +K+KSRL +PP P   ++  +KT+ KS   S  + S  +           
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTPTHPAI--DKTEMKSGRRSGIFKSGFLGK--------- 244

Query: 141 PREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCL 200
                     +PK    G  G EEE++E+ +   +L  +E    KL   + +EW++ + +
Sbjct: 245 ----------SPKAGTPGRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWISLVLI 304

Query: 201 TGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRK 260
              L+ SLT+  L  K  W L +WKW V VLV+ CGRL S W + I+VFL+E+NF  +++
Sbjct: 305 VTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKR 364

Query: 261 VLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAALW 320
           VLYFVYG+RKSV   +WL LVLLAW  LFD+  +R  + +  L YVTR L   L+   +W
Sbjct: 365 VLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIW 424

Query: 321 LVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPPLMEM---------------- 380
           LVKT+LVK+LA+SF  + +FDRIQES+F QY++ TLSGPPLME+                
Sbjct: 425 LVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKS 484

Query: 381 -----AERVGRAASTGQLSFRHLKKESD----GGNEGKE-EVIDVDKLKKMKQEKISAWT 440
                  ++  A      SF  + K       G   G++ E I +D+LK+M  + +SAW 
Sbjct: 485 LEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWN 544

Query: 441 MRGLINVIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYI 500
           M+ L+N+I    +ST+   +++  +E  ++    I SE+EA+ AA +IF NV +PGS+YI
Sbjct: 545 MKRLMNIILKGAISTLDQNMQDTTQE--DEDATHIRSEYEAKCAARKIFHNVTEPGSRYI 604

Query: 501 DEEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAI 560
             ED  RF+ +EE +  + LFEGA E+ KI +  LKNW+V  + ER++LA +LNDTKTA+
Sbjct: 605 YLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAV 664

Query: 561 EELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVF 620
           + L+++ + ++ I+III WLL++G  TT+ L+ +SSQLLLV F+FGN+ +T+FEAIIF+F
Sbjct: 665 DRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLF 724

Query: 621 VMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMS 680
           VMHPFDVGDRC +DGVQ++VEEMNILTT+FLRYDN+KI YPNSVL TKPI+N+YRSP+M 
Sbjct: 725 VMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMG 784

Query: 681 DSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHT 740
           D++EF V  +T  E I A+K RI SY+++K  +W P   I+   ++++N +K+ +++ H 
Sbjct: 785 DAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHR 844

Query: 741 INFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYV-----SSAAPMVPPS 768
           +N Q+ G++  RR  L+ E+ K   +L I+Y L P  + +  +      +++  +PPS
Sbjct: 845 MNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTANPTSSDRIPPS 874

BLAST of Tan0010204.1 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 6.3e-148
Identity = 317/766 (41.38%), Postives = 461/766 (60.18%), Query Frame = 0

Query: 44  RSFRCTIPQSVV-GSSPSHEISRMSPLKPPKIPV-ESAVRRPSLARSSFSKSKSRLVEPP 103
           R  R T+  SV  G S   E +R+   +  K     S +R  +L +    K++SRL++PP
Sbjct: 138 RQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMK---MKTRSRLMDPP 197

Query: 104 CPDGLSLAEEKTQAKSTSGSAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTG 163
            P    +   +T         F                       + P TP     GS  
Sbjct: 198 TPTYPDMVSGRTPRSGNLNPGF-------------------SGRNTKPGTPNQG--GSKD 257

Query: 164 SEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGL 223
            EEE+D   +   +L    R  +K+   ++IEW+  + +   LI SL +  L  K +W L
Sbjct: 258 LEEEEDP--FSEEDLPEGLRK-EKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDL 317

Query: 224 GMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALV 283
            +WKW V+VLV+ CGRL S W + + V+ +E NFL ++KVLYFVYG+RK V   +WL LV
Sbjct: 318 ALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLV 377

Query: 284 LLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFD 343
           L+AW  LFD+  +R  + + +L YVT+ L   L+   +WL+KTLLVK+LA+SF  + +FD
Sbjct: 378 LIAWHFLFDKKVEREMR-STVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFD 437

Query: 344 RIQESIFHQYILRTLSGPPLMEM---AERVGRAASTGQLSFRHLK--------------- 403
           RIQES+F QY++ TLSGPP +E+    E+V     T ++  R L                
Sbjct: 438 RIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTV 497

Query: 404 ------------------KESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSS 463
                               S    EG EE I +D L++M  + +SAW M+ L+NVI+  
Sbjct: 498 GSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKG 557

Query: 464 GLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK 523
            LST+   I++   +E + K  +I SE+EA+ AA +IF+NVA+PGS+YI  ED  RF+S+
Sbjct: 558 TLSTLDEQIQDTTTQE-DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSE 617

Query: 524 EEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASALV 583
           +E +  + LFEGA E  KI +  LKNW+VN + ER++LA +LNDTKTA+  L+++   LV
Sbjct: 618 DESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLV 677

Query: 584 LIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC 643
            IVI+I WLL++G  TT+ L+ ISSQLLLVVF+FGN+ +T+FEA+IFVFVMHPFDVGDRC
Sbjct: 678 SIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRC 737

Query: 644 VVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFST 703
            +DGVQMIVEEMNILTT+FLR+DN+KI YPNS+L TKPI+N+YRSP+M D+IEF V  +T
Sbjct: 738 EIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIAT 797

Query: 704 SIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNYGDKSS 763
             E   AL+ RI SY+++K   W P+  I+ +++  +N +K+ ++  H +N QN G++  
Sbjct: 798 PPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYV 857

Query: 764 RRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPM----VPPS 768
           RR +L+ E+ ++  +L I+Y L P  + +  + +A P+    +PPS
Sbjct: 858 RRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPS 874

BLAST of Tan0010204.1 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.1e-144
Identity = 291/649 (44.84%), Postives = 425/649 (65.49%), Query Frame = 0

Query: 151 TPKTPLIGSTGSEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTV 210
           +PKT      G EEEDD      AE   +E    KL   I++EW++ + +    + +L +
Sbjct: 211 SPKT-----QGEEEEDDP---FAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAI 270

Query: 211 EKLVTKEIWGLGMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRK 270
             L  K++W L +WKW  +VLV+ CGRL S W + I+VF IERNFLL+++VLYFVYG+RK
Sbjct: 271 PSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRK 330

Query: 271 SVIIFIWLALVLLAWGLLFDQSSKRSKKGN-EILNYVTRALGASLIGAALWLVKTLLVKI 330
           +V   +WL LVLLAW  LFD+  K +K  N + L  VT+     L+G  LWLVKTLLVK+
Sbjct: 331 AVQNCLWLGLVLLAWHFLFDE--KVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKV 390

Query: 331 LAASFQCARFFDRIQESIFHQYILRTLSGPPLMEM------AERVG-------------- 390
           LA+SF  + +FDRIQES+F QY++ TLSGPPL+E+       ER+               
Sbjct: 391 LASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEI 450

Query: 391 ------RAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 450
                     TG+  F      + GG  G+ + I +D L K+  + +SAW M+ L+N+IR
Sbjct: 451 QSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIR 510

Query: 451 SSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 510
           +  L+T+   +++   +  + K  +I SE+EA+ AA +IF NVAKPGSK+I   D+ RF+
Sbjct: 511 NGSLTTLDEQLQDPSLD--DDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFL 570

Query: 511 SKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 570
             +E    L LFEGA ET +I + SLKNW+VN + ER++LA +LNDTKTA+  L+K+ + 
Sbjct: 571 PDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNI 630

Query: 571 LVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 630
           +V I+I++ WL+++G  +T+ LV +SSQ+++V F+FGN  + VFE+II++FV+HPFDVGD
Sbjct: 631 VVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGD 690

Query: 631 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDF 690
           RC +DGVQM+VEEMNILTT+FLR+DN+K+ YPNS+L TK I N+YRSP+M D IEFS+  
Sbjct: 691 RCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHI 750

Query: 691 STSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNYGDK 750
           +T  E I  +K RI SYIE K   W P   I+ K++E++N +++ ++  H +N Q+ G+K
Sbjct: 751 TTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEK 810

Query: 751 SSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYV--SSAAPM---VPPS 768
            +RRS+LV E+ KI  +L I+Y L P ++ +  +  S+A P+   +PP+
Sbjct: 811 WARRSQLVEEIAKICRELDIEYRLYPLDINVRNLPTSTALPVSDRLPPN 847

BLAST of Tan0010204.1 vs. NCBI nr
Match: XP_038878911.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1347.8 bits (3487), Expect = 0.0e+00
Identity = 698/769 (90.77%), Postives = 736/769 (95.71%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADKKG EQ+VLRIL+GE  VD SKD  K SV+SFPDF+LKETRSFRCTIPQSVVGSSPS
Sbjct: 1   MADKKGMEQLVLRILEGEEGVDTSKDLTKPSVSSFPDFDLKETRSFRCTIPQSVVGSSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
           HEISRMSPLKPPKIP E+A+RRPS ARSSFSK KSRL+E PCPD  SLAEEK +AKST  
Sbjct: 61  HEISRMSPLKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDSASLAEEKAKAKST-- 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
              YSSPKMDSPAKITT TSP+EALKSAPITPKTPLIG+TGSEEEDDEEVY+TAELKVKE
Sbjct: 121 --LYSSPKMDSPAKITTVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           RSGKKLKRT+LIEWVAFLCLTGCLIASLT++KLVTKEIWGLG+WKWCVLVLV FCGRLFS
Sbjct: 181 RSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVSFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLLKRKVLYFVYGLRKSVIIFIWL LVLLAWGLLFDQSSKRSKKGN
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLGLVLLAWGLLFDQSSKRSKKGN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           EILNY+TRALGASLIGA LWLVKTL+VKILAASFQCARFFDRIQESIFHQYILR LSGPP
Sbjct: 301 EILNYITRALGASLIGAGLWLVKTLMVKILAASFQCARFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           +MEMAE VGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN
Sbjct: 361 IMEMAESVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDE+DLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEDDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFMSKEE+DNVLPLFEG VETGKIKRK+LKNWLVNVY+ERKSLAHSLNDTKTAIEELNKL
Sbjct: 481 RFMSKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNKL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA+VLIVIIIEWLLLMGFLTTQVLVF+SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFD
Sbjct: 541 ASAIVLIVIIIEWLLLMGFLTTQVLVFVSSQILLVVFMFGNTARTVFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSIEFS
Sbjct: 601 VGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIEFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIK+Y+ESKPQFWRPNHS++VKEIEN+NKMK+ L VNHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKTYLESKPQFWRPNHSVIVKEIENINKMKLALCVNHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMVPPSRR 770
           GDKSSRRS+LVLE+KKIFEDLGIKYHLLPQEVQLNYV SAA ++P S+R
Sbjct: 721 GDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVGSAASVIPSSQR 765

BLAST of Tan0010204.1 vs. NCBI nr
Match: XP_022987285.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 687/769 (89.34%), Postives = 721/769 (93.76%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADKKGTEQVVLRILDGE  VD        SVASFPDFE KETRS RC IPQSVV SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
           HEISRMS LKPPKIPVESAVRRPS ARSSFSK KSRL+EPPCPDG S AEE  QAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
           S F SSPKMDSPAKI TATSP+E+LKSAPITP+TPL+GSTGSEEEDDEEVY+TAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           +S KKLKR +LIEW+AFLC+T CLI+SLT++KL+TKEIWGLG+WKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLLKRKVLYFVYGLRKSVI+FIWLALVLLAWGLLFDQS KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRAL ASLIGA LWLVKTLLVKILAASFQC RFFDRIQESIFHQYILR LSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           LMEMAERVGR AS GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE+DNVLPLFEG VETGKIKRK+LKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIII WLLLMGFLTTQVLVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSY+ESKPQFWRPNHS++VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMVPPSRR 770
           GD+SSRRS+LVLE+KKIFEDLGIKYHLLPQEVQLNYVSS APMV  + R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of Tan0010204.1 vs. NCBI nr
Match: XP_022147669.1 (mechanosensitive ion channel protein 10-like [Momordica charantia])

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 693/769 (90.12%), Postives = 723/769 (94.02%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADK+GTEQVVLRILD EGAVDG+KD  K SVAS PDFELKETRS RCTIPQS VGSSPS
Sbjct: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
            EI+RMSPLKPPKIPVES VRRPS ARSSFSK KSRLVEPPCP G +LAE+K QAKSTSG
Sbjct: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
                SP  +SPAKITTATSPR+ALKSAPITPKTPLIGS GSEEEDDEEVY+TAELKVKE
Sbjct: 121 -----SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           RSG+KLK TILIEWVAFLC+TGC IASLT+ KL T EIWGLG+W+WCVLVLVIFCGRLFS
Sbjct: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLL+RKVLYFVYGLRKSVIIFIWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRAL ASL GA LWL+KTLLVKILAASFQC RFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           L+EMAERVGR ASTGQLSFRHLKK+SDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN
Sbjct: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFMSKEEV+NVLPLFEGAVETGKIKRK+LKNWLVNVYVERKSLAHSLNDTKTAIEELNKL
Sbjct: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA+VLIVIII WLLLMG LTTQ+LVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD
Sbjct: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS
Sbjct: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSY+ESKPQFWRP HS++VKEIENVNKMKMGLYVNHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMVPPSRR 770
           GDKSSRRS+LVLE+KKI E+LGIKYHLLPQEV LN V SAAPMVPPS R
Sbjct: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764

BLAST of Tan0010204.1 vs. NCBI nr
Match: XP_022960737.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022960738.1 mechanosensitive ion channel protein 10-like [Cucurbita moschata])

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 683/769 (88.82%), Postives = 720/769 (93.63%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADKKG EQVVLRILDGE  VD        SVASFPDFE KETRS RC I QSVV SSPS
Sbjct: 1   MADKKGMEQVVLRILDGEEGVDARNGLTNGSVASFPDFEPKETRSLRCAITQSVVESSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
           HEISRMS  KPPKIPVESAVRRPS ARSSFSK KSRL+EPP PDG SLAEE  QAKS SG
Sbjct: 61  HEISRMSSSKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPRPDGASLAEENAQAKSASG 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
           S F SSPKMDSPAKI TATSP+E+LKSAPITP+TPL+GSTGSEEEDDEEVY+TAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           +S KKLKR ++IEW+AFLC+T CLI+SLT++KL+TKEIWGLG+WKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVVIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLLKRKVLYFVYGLRKSVI+FIWLALVLLAWGLLFDQS KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRAL ASLIGA LWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           LMEMAERVG AAS+GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQEK+SAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE+DNVLPLFEG VETGKIKRK+LKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIII WLLLMGFLTTQVLVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSY+ESKPQFWRPNHS++VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMVPPSRR 770
           GD+SSRRS+LVLE+KKIFEDLGIKYHLLPQEVQLNY+SS APMV  + R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYLSSVAPMVSTTLR 769

BLAST of Tan0010204.1 vs. NCBI nr
Match: KAG7023687.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1311.6 bits (3393), Expect = 0.0e+00
Identity = 680/757 (89.83%), Postives = 715/757 (94.45%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADKKGTEQVVLRILDGE  VD   D    SVAS PDFE KETRS RC IPQSVV SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
           HEISRMS LKPPKIPV SAVRRPS ARSSFSK KSRL+EPPCPDG SLAEE  QAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVGSAVRRPSFARSSFSKPKSRLIEPPCPDGASLAEENAQAKSTSG 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
           S F SSPKMDSPA I TATSP+E+LKSAPITP+TPL+GSTGSEEEDDEEVY+TAELKVKE
Sbjct: 121 SPFSSSPKMDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           +S KKLKR +LIEW+AFLC+T CLI+SLT++KL+TKEIWGLG+WKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLLKRKVLYFVYGLRKSVI+FIWLALVLLAWGLLFDQS KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRAL ASLIGA LWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           LMEMAERVG AAS+GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQEK+SAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE+DNVLPLFEG VETGKIKRK+LKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIII WLLLMGFLTTQVLVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSY+ESKPQFWRPNHS++VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYV 758
           GD+SSRRS+LVLE+KKIFEDLGIKYHLLPQEVQLNY+
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYL 757

BLAST of Tan0010204.1 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 687/769 (89.34%), Postives = 721/769 (93.76%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADKKGTEQVVLRILDGE  VD        SVASFPDFE KETRS RC IPQSVV SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
           HEISRMS LKPPKIPVESAVRRPS ARSSFSK KSRL+EPPCPDG S AEE  QAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
           S F SSPKMDSPAKI TATSP+E+LKSAPITP+TPL+GSTGSEEEDDEEVY+TAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           +S KKLKR +LIEW+AFLC+T CLI+SLT++KL+TKEIWGLG+WKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLLKRKVLYFVYGLRKSVI+FIWLALVLLAWGLLFDQS KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRAL ASLIGA LWLVKTLLVKILAASFQC RFFDRIQESIFHQYILR LSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           LMEMAERVGR AS GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE+DNVLPLFEG VETGKIKRK+LKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIII WLLLMGFLTTQVLVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSY+ESKPQFWRPNHS++VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMVPPSRR 770
           GD+SSRRS+LVLE+KKIFEDLGIKYHLLPQEVQLNYVSS APMV  + R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of Tan0010204.1 vs. ExPASy TrEMBL
Match: A0A6J1D1P7 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111016536 PE=3 SV=1)

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 693/769 (90.12%), Postives = 723/769 (94.02%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADK+GTEQVVLRILD EGAVDG+KD  K SVAS PDFELKETRS RCTIPQS VGSSPS
Sbjct: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
            EI+RMSPLKPPKIPVES VRRPS ARSSFSK KSRLVEPPCP G +LAE+K QAKSTSG
Sbjct: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
                SP  +SPAKITTATSPR+ALKSAPITPKTPLIGS GSEEEDDEEVY+TAELKVKE
Sbjct: 121 -----SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           RSG+KLK TILIEWVAFLC+TGC IASLT+ KL T EIWGLG+W+WCVLVLVIFCGRLFS
Sbjct: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLL+RKVLYFVYGLRKSVIIFIWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRAL ASL GA LWL+KTLLVKILAASFQC RFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           L+EMAERVGR ASTGQLSFRHLKK+SDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN
Sbjct: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFMSKEEV+NVLPLFEGAVETGKIKRK+LKNWLVNVYVERKSLAHSLNDTKTAIEELNKL
Sbjct: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA+VLIVIII WLLLMG LTTQ+LVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD
Sbjct: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS
Sbjct: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSY+ESKPQFWRP HS++VKEIENVNKMKMGLYVNHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMVPPSRR 770
           GDKSSRRS+LVLE+KKI E+LGIKYHLLPQEV LN V SAAPMVPPS R
Sbjct: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764

BLAST of Tan0010204.1 vs. ExPASy TrEMBL
Match: A0A6J1H896 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111461449 PE=3 SV=1)

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 683/769 (88.82%), Postives = 720/769 (93.63%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPS 60
           MADKKG EQVVLRILDGE  VD        SVASFPDFE KETRS RC I QSVV SSPS
Sbjct: 1   MADKKGMEQVVLRILDGEEGVDARNGLTNGSVASFPDFEPKETRSLRCAITQSVVESSPS 60

Query: 61  HEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSG 120
           HEISRMS  KPPKIPVESAVRRPS ARSSFSK KSRL+EPP PDG SLAEE  QAKS SG
Sbjct: 61  HEISRMSSSKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPRPDGASLAEENAQAKSASG 120

Query: 121 SAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKE 180
           S F SSPKMDSPAKI TATSP+E+LKSAPITP+TPL+GSTGSEEEDDEEVY+TAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFS 240
           +S KKLKR ++IEW+AFLC+T CLI+SLT++KL+TKEIWGLG+WKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVVIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFIN LVFLIERNFLLKRKVLYFVYGLRKSVI+FIWLALVLLAWGLLFDQS KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRAL ASLIGA LWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           LMEMAERVG AAS+GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQEK+SAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE+DNVLPLFEG VETGKIKRK+LKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIII WLLLMGFLTTQVLVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSY+ESKPQFWRPNHS++VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMVPPSRR 770
           GD+SSRRS+LVLE+KKIFEDLGIKYHLLPQEVQLNY+SS APMV  + R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYLSSVAPMVSTTLR 769

BLAST of Tan0010204.1 vs. ExPASy TrEMBL
Match: A0A0A0LYZ9 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 PE=3 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 680/763 (89.12%), Postives = 725/763 (95.02%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDG-EGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSP 60
           MADKKG EQ+VLRIL+G EG  + SKD  K SV SFPDF+LKETRSFRCTIPQSVVGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTS 120
           SHEISRM+P KPPKIP E+  RR S A SSF+K KSRL+EPPCPDG SLAEEK  AKST 
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKST- 120

Query: 121 GSAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVK 180
               YSS K+DSPAKITT TSP+EALK+APITPKTPLIG+TG+EEEDDEEVYRTAELKVK
Sbjct: 121 ---LYSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVK 180

Query: 181 ERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLF 240
           E+SGK+LK+T+++EW+AFLCLTGCLIASLT+E LVTKEIWGLG+WKWCVLVLVIFCGRLF
Sbjct: 181 EKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKG 300
           SQWFIN LVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSK+G
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEG 300

Query: 301 NEILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGP 360
           N+ILNYVTRALGASLIGA LWLVKTLLVKILAASFQC RFFDRIQESIFHQYILR LSGP
Sbjct: 301 NKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420
           PLMEMAERVGRAASTGQLSF+HLKKESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLI
Sbjct: 361 PLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420

Query: 421 NVIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540
           FRFMSKEE+DNVLPLFEG  ETGKIKRK+LKNWLVNVYVERKSLAHSLNDTKTAIEELNK
Sbjct: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540

Query: 541 LASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPF 600
           L+SA++LIVIIIEWLLLMGFLTTQVLVFISSQ+LLVVFMFGNTARTVFEAIIFVFVMHPF
Sbjct: 541 LSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEF 660
           DVGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSI+F
Sbjct: 601 DVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQN 720
           SVDFSTSIESIGALKARIK+Y+ESKPQFWRPN+S++VKEIENVNKMK+ L VNHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQN 720

Query: 721 YGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAP 763
           YGDKS+RRS+LVLE+KKIFE+LGIKYHLLPQEVQLNYVSSAAP
Sbjct: 721 YGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAP 759

BLAST of Tan0010204.1 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 681/772 (88.21%), Postives = 725/772 (93.91%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEGAVDG-SKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSP 60
           MADKKG EQ+VLRIL+GE  V G SKD  K SV SFPDF+LKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMSPLKPPKIPVESAVRRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTS 120
           SHEISRM+PLKPPKIP E+  RR S A SSFSK KSRL+EPPCPDG SLAEEK  AK   
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAK--- 120

Query: 121 GSAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVK 180
            S+ Y SPK+DSPAKIT  TSP+EALK+ PITPKTPLIG+TG+EEEDDEEVY+TAELKVK
Sbjct: 121 -SSLYGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVK 180

Query: 181 ERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLF 240
           E+S K+LK+T+++EWVAFLCLTGCLIASLT++ LVTKEIWGLG+WKWCVLVLVIFCGRLF
Sbjct: 181 EKSEKRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKG 300
           SQWFIN LVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSKKG
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKG 300

Query: 301 NEILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGP 360
           NEILNYVTRALGASLIGA LWLVKTLLVKILAASFQC RFFDRIQESIFHQYILR LSGP
Sbjct: 301 NEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420
           PLMEMA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLI
Sbjct: 361 PLMEMAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420

Query: 421 NVIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540
           FRFMSKEE+DNVLPLFEG  ETGKIKRK+LKNWLVNVYVERKSLAHSLNDTKTAIEELNK
Sbjct: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540

Query: 541 LASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPF 600
           LASA+VLIVIIIEWLLLMGFLTTQVLVFISSQ+LLVVFMFGNTARTVFEAIIFVFVMHPF
Sbjct: 541 LASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIEF 660
           DVGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSI+F
Sbjct: 601 DVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTINFQN 720
           SVDFSTSIESIGALKARIK+Y+ESKPQFWRPN+S++VKEIENVNKMK+ L VNHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQN 720

Query: 721 YGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYVSSAAPMV--PPSRR 770
           YGDKS+RRS+LVLE+KKIFEDLGIKYHLLPQ VQLNY SSAA  V  PPS+R
Sbjct: 721 YGDKSNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of Tan0010204.1 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 688.7 bits (1776), Expect = 5.3e-198
Identity = 382/721 (52.98%), Postives = 513/721 (71.15%), Query Frame = 0

Query: 44  RSFRCTIPQSVVG----SSPSHEISRM--SPLKPPKIPVESAV---RRPSLARSSFSKSK 103
           RS     P+S  G     SPS EIS++  SP KPP+ P ++ V   +R S ARS +SK K
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPDGLSLAEEKTQAKSTSGSAFYSSPKMDSPAKITTATSPREALKSAPITPKT 163
           SR V+P CP   S+ EE+ + +  +G +F  +    SP       S R     AP+TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRA----SP----NNKSNRSVGSPAPVTPSK 147

Query: 164 PLIGSTGSEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLV 223
            ++     E+++DEE+Y+  +L  + RS  K+    LIE   F+ +   L+ASLT+  L 
Sbjct: 148 VVV-----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLK 207

Query: 224 TKEIWGLGMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRKSVII 283
               WGL +WKWCVLV+VIF G L + WF+ ++VFLIE NFLL+RKVLYFV+GL+KSV +
Sbjct: 208 HHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQV 267

Query: 284 FIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAALWLVKTLLVKILAASF 343
           FIWL L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F
Sbjct: 268 FIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANF 327

Query: 344 QCARFFDRIQESIFHQYILRTLSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKE 403
               FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  STG LSF  + K+   G   ++
Sbjct: 328 NVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEK 387

Query: 404 EVIDVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEEVEQKDKEINSEW 463
           +VID+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE 
Sbjct: 388 KVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEM 447

Query: 464 EARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWL 523
           EA AAAY +FRNVA+P   YI+EEDL RFM KEEVD V PLF+GA ETG+I RK+   W+
Sbjct: 448 EALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWV 507

Query: 524 VNVYVERKSLAHSLNDTKTAIEELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLL 583
           V VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+QL+
Sbjct: 508 VKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLV 567

Query: 584 LVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIF 643
            + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++
Sbjct: 568 ALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVY 627

Query: 644 YPNSVLATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHS 703
           YPN+VLATKPISN++RSP M +++EFS+ FST +  I  LK RI  Y+E  PQ W P HS
Sbjct: 628 YPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHS 687

Query: 704 ILVKEIENVNKMKMGLYVNHTINFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQ 755
           ++VKEIEN+NK+KM LY +HTI FQ   +++ RR+EL L +K++ EDL I Y LLPQ++ 
Sbjct: 688 VVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDIN 730

BLAST of Tan0010204.1 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 688.7 bits (1776), Expect = 5.3e-198
Identity = 382/721 (52.98%), Postives = 513/721 (71.15%), Query Frame = 0

Query: 44  RSFRCTIPQSVVG----SSPSHEISRM--SPLKPPKIPVESAV---RRPSLARSSFSKSK 103
           RS     P+S  G     SPS EIS++  SP KPP+ P ++ V   +R S ARS +SK K
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPDGLSLAEEKTQAKSTSGSAFYSSPKMDSPAKITTATSPREALKSAPITPKT 163
           SR V+P CP   S+ EE+ + +  +G +F  +    SP       S R     AP+TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRA----SP----NNKSNRSVGSPAPVTPSK 147

Query: 164 PLIGSTGSEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLV 223
            ++     E+++DEE+Y+  +L  + RS  K+    LIE   F+ +   L+ASLT+  L 
Sbjct: 148 VVV-----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLK 207

Query: 224 TKEIWGLGMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRKSVII 283
               WGL +WKWCVLV+VIF G L + WF+ ++VFLIE NFLL+RKVLYFV+GL+KSV +
Sbjct: 208 HHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQV 267

Query: 284 FIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAALWLVKTLLVKILAASF 343
           FIWL L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F
Sbjct: 268 FIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANF 327

Query: 344 QCARFFDRIQESIFHQYILRTLSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKE 403
               FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  STG LSF  + K+   G   ++
Sbjct: 328 NVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEK 387

Query: 404 EVIDVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEEVEQKDKEINSEW 463
           +VID+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE 
Sbjct: 388 KVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEM 447

Query: 464 EARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWL 523
           EA AAAY +FRNVA+P   YI+EEDL RFM KEEVD V PLF+GA ETG+I RK+   W+
Sbjct: 448 EALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWV 507

Query: 524 VNVYVERKSLAHSLNDTKTAIEELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLL 583
           V VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+QL+
Sbjct: 508 VKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLV 567

Query: 584 LVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIF 643
            + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++
Sbjct: 568 ALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVY 627

Query: 644 YPNSVLATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHS 703
           YPN+VLATKPISN++RSP M +++EFS+ FST +  I  LK RI  Y+E  PQ W P HS
Sbjct: 628 YPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHS 687

Query: 704 ILVKEIENVNKMKMGLYVNHTINFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQ 755
           ++VKEIEN+NK+KM LY +HTI FQ   +++ RR+EL L +K++ EDL I Y LLPQ++ 
Sbjct: 688 VVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDIN 730

BLAST of Tan0010204.1 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 688.7 bits (1776), Expect = 5.3e-198
Identity = 382/721 (52.98%), Postives = 513/721 (71.15%), Query Frame = 0

Query: 44  RSFRCTIPQSVVG----SSPSHEISRM--SPLKPPKIPVESAV---RRPSLARSSFSKSK 103
           RS     P+S  G     SPS EIS++  SP KPP+ P ++ V   +R S ARS +SK K
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPDGLSLAEEKTQAKSTSGSAFYSSPKMDSPAKITTATSPREALKSAPITPKT 163
           SR V+P CP   S+ EE+ + +  +G +F  +    SP       S R     AP+TP  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRA----SP----NNKSNRSVGSPAPVTPSK 147

Query: 164 PLIGSTGSEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLV 223
            ++     E+++DEE+Y+  +L  + RS  K+    LIE   F+ +   L+ASLT+  L 
Sbjct: 148 VVV-----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLK 207

Query: 224 TKEIWGLGMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRKSVII 283
               WGL +WKWCVLV+VIF G L + WF+ ++VFLIE NFLL+RKVLYFV+GL+KSV +
Sbjct: 208 HHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQV 267

Query: 284 FIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAALWLVKTLLVKILAASF 343
           FIWL L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F
Sbjct: 268 FIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANF 327

Query: 344 QCARFFDRIQESIFHQYILRTLSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKE 403
               FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  STG LSF  + K+   G   ++
Sbjct: 328 NVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEK 387

Query: 404 EVIDVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEEVEQKDKEINSEW 463
           +VID+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE 
Sbjct: 388 KVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEM 447

Query: 464 EARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWL 523
           EA AAAY +FRNVA+P   YI+EEDL RFM KEEVD V PLF+GA ETG+I RK+   W+
Sbjct: 448 EALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWV 507

Query: 524 VNVYVERKSLAHSLNDTKTAIEELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLL 583
           V VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+QL+
Sbjct: 508 VKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLV 567

Query: 584 LVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIF 643
            + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++
Sbjct: 568 ALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVY 627

Query: 644 YPNSVLATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHS 703
           YPN+VLATKPISN++RSP M +++EFS+ FST +  I  LK RI  Y+E  PQ W P HS
Sbjct: 628 YPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHS 687

Query: 704 ILVKEIENVNKMKMGLYVNHTINFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQ 755
           ++VKEIEN+NK+KM LY +HTI FQ   +++ RR+EL L +K++ EDL I Y LLPQ++ 
Sbjct: 688 VVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDIN 730

BLAST of Tan0010204.1 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 618.6 bits (1594), Expect = 6.7e-177
Identity = 354/759 (46.64%), Postives = 499/759 (65.74%), Query Frame = 0

Query: 8   EQVVLRILDGEGAVDGSKDFPKSSVASFPDFELKETRSFRCTIPQSVVGSSPSHEISRMS 67
           E+VV+ + D E + D  +  P  +  + PD +    +S +   P S+    P+ EI + S
Sbjct: 10  EEVVINVSDKEDSKD-PRASPSFNPLASPDSDAGIEKS-KPVPPISI----PTPEIYKFS 69

Query: 68  PL--KPPKIP-VESAVRRPSLARSSFSKSKSRLVEPPC---------PDGLSLAEEKTQA 127
               KPPKIP  E  VRR SL+RS +SK KSR  E              G SL E+    
Sbjct: 70  GSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAG 129

Query: 128 KSTSGSAFYSSPKMDSPAKITTATSPREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAE 187
               GS   +SP   S   + +A   + A                  E +++EE+Y+  +
Sbjct: 130 SFARGSFDRASPNNKSNRSVASAALSKVA----------------EEEPDENEEIYKKVK 189

Query: 188 LKVKERSGKKLKRTILIEWVAFLCLTGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFC 247
           L   +RSG  +K    +E V F+ + G LI SLT++ +    IWGL  WKWCVLV+V   
Sbjct: 190 LHRVKRSG--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLS 249

Query: 248 GRLFSQWFINILVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKR 307
           G L + WF++ +VF+IE+N+LL++KVLYFV+GL+K+V +FIW +LVL+AW  LFD   KR
Sbjct: 250 GMLVTNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKR 309

Query: 308 SKKGNEILNYVTRALGASLIGAALWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRT 367
           ++K    L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+T
Sbjct: 310 TRKTKRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQT 369

Query: 368 LSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTM 427
           LSGPPL+E AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTM
Sbjct: 370 LSGPPLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTM 429

Query: 428 RGLINVIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYID 487
           R LI  + +SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+
Sbjct: 430 RVLIEAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIE 489

Query: 488 EEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAIE 547
           E+DL RFM KEEVD VLPL E A +TGKI RK+   W+VNVY  RK++ HSLNDTKTA++
Sbjct: 490 EDDLLRFMIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVK 549

Query: 548 ELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVFV 607
           +L+KL + ++ ++  I W++L+   +T++L+  SSQ L + FM G+T + +FE+ +FVFV
Sbjct: 550 QLDKLITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFV 609

Query: 608 MHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSD 667
           MHP+DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+M D
Sbjct: 610 MHPYDVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGD 669

Query: 668 SIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHTI 727
            ++F + FST  E IG LK +I  Y+ +  Q W P   ++V+ IEN+NK+ + + V HTI
Sbjct: 670 YVDFGIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTI 729

Query: 728 NFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQL 755
           NFQ Y +KS RR+ L++ +K+I EDL I Y LLPQ+V L
Sbjct: 730 NFQVYVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of Tan0010204.1 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 532.7 bits (1371), Expect = 4.8e-151
Identity = 303/718 (42.20%), Postives = 453/718 (63.09%), Query Frame = 0

Query: 81  RRPSLARSSFSKSKSRLVEPPCPDGLSLAEEKTQAKSTSGSAFYSSPKMDSPAKITTATS 140
           ++P L+R   +K+KSRL +PP P   ++  +KT+ KS   S  + S  +           
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTPTHPAI--DKTEMKSGRRSGIFKSGFLGK--------- 244

Query: 141 PREALKSAPITPKTPLIGSTGSEEEDDEEVYRTAELKVKERSGKKLKRTILIEWVAFLCL 200
                     +PK    G  G EEE++E+ +   +L  +E    KL   + +EW++ + +
Sbjct: 245 ----------SPKAGTPGRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWISLVLI 304

Query: 201 TGCLIASLTVEKLVTKEIWGLGMWKWCVLVLVIFCGRLFSQWFINILVFLIERNFLLKRK 260
              L+ SLT+  L  K  W L +WKW V VLV+ CGRL S W + I+VFL+E+NF  +++
Sbjct: 305 VTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKR 364

Query: 261 VLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAALW 320
           VLYFVYG+RKSV   +WL LVLLAW  LFD+  +R  + +  L YVTR L   L+   +W
Sbjct: 365 VLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIW 424

Query: 321 LVKTLLVKILAASFQCARFFDRIQESIFHQYILRTLSGPPLMEM---------------- 380
           LVKT+LVK+LA+SF  + +FDRIQES+F QY++ TLSGPPLME+                
Sbjct: 425 LVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKS 484

Query: 381 -----AERVGRAASTGQLSFRHLKKESD----GGNEGKE-EVIDVDKLKKMKQEKISAWT 440
                  ++  A      SF  + K       G   G++ E I +D+LK+M  + +SAW 
Sbjct: 485 LEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWN 544

Query: 441 MRGLINVIRSSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYI 500
           M+ L+N+I    +ST+   +++  +E  ++    I SE+EA+ AA +IF NV +PGS+YI
Sbjct: 545 MKRLMNIILKGAISTLDQNMQDTTQE--DEDATHIRSEYEAKCAARKIFHNVTEPGSRYI 604

Query: 501 DEEDLFRFMSKEEVDNVLPLFEGAVETGKIKRKSLKNWLVNVYVERKSLAHSLNDTKTAI 560
             ED  RF+ +EE +  + LFEGA E+ KI +  LKNW+V  + ER++LA +LNDTKTA+
Sbjct: 605 YLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAV 664

Query: 561 EELNKLASALVLIVIIIEWLLLMGFLTTQVLVFISSQLLLVVFMFGNTARTVFEAIIFVF 620
           + L+++ + ++ I+III WLL++G  TT+ L+ +SSQLLLV F+FGN+ +T+FEAIIF+F
Sbjct: 665 DRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLF 724

Query: 621 VMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMS 680
           VMHPFDVGDRC +DGVQ++VEEMNILTT+FLRYDN+KI YPNSVL TKPI+N+YRSP+M 
Sbjct: 725 VMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMG 784

Query: 681 DSIEFSVDFSTSIESIGALKARIKSYIESKPQFWRPNHSILVKEIENVNKMKMGLYVNHT 740
           D++EF V  +T  E I A+K RI SY+++K  +W P   I+   ++++N +K+ +++ H 
Sbjct: 785 DAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHR 844

Query: 741 INFQNYGDKSSRRSELVLEMKKIFEDLGIKYHLLPQEVQLNYV-----SSAAPMVPPS 768
           +N Q+ G++  RR  L+ E+ K   +L I+Y L P  + +  +      +++  +PPS
Sbjct: 845 MNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTANPTSSDRIPPS 874