Tan0007499.1 (mRNA) Snake gourd v1

BLAST of Tan0007499.1 vs. ExPASy Swiss-Prot
Match: Q4VYC8 (Protein NODULATION SIGNALING PATHWAY 1 OS=Medicago truncatula OX=3880 GN=NSP1 PE=1 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.5e-183
Identity = 344/561 (61.32%), Postives = 414/561 (73.80%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLDETYNSSSINCYQWWDENQDI--------- 60
           MT+E   PN  SDHILDWLE SV FF  FLD+ YN+  I+ Y+ W++NQDI         
Sbjct: 1   MTME---PNPTSDHILDWLEGSVSFFPSFLDDPYNNGYIHEYEIWNQNQDISNQYQIDAN 60

Query: 61  --GQDLINGCLSGSPTTVTTVTTRLPNIPTSHHLTP-SDLTKKRKAPDDTVHKKSQPHQN 120
               +  N   +    + TT TT L   P S +  P SDL KKR A D+   KK QP   
Sbjct: 61  TNSSNATNSTTNIVAASTTTSTTSLE--PNSFNNIPFSDLPKKRNAEDELSLKK-QPQNQ 120

Query: 121 QRKNQNNQSKNGADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLN 180
           + K   ++  N +D G+  +E  TV++K+ GNKK ++K+ G+N NNG+NK+GRWAEQLLN
Sbjct: 121 KNKRLKSRPMNESDNGDAALEG-TVVRKSGGNKKGAAKANGSNSNNGNNKDGRWAEQLLN 180

Query: 181 PCANAIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSST 240
           PCA AI  G+  RV HLL VL ELAS TGDANHRLAAHGLRAL HHLSS+SSS+ S +  
Sbjct: 181 PCAVAITGGNLNRVQHLLYVLHELASTTGDANHRLAAHGLRALTHHLSSSSSSTPSGT-- 240

Query: 241 VAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGV 300
              +TFAST+PRFFQ+SL+KF+E SPWF+FPNNIAN+SIL  L+EEPN  R LHILDIGV
Sbjct: 241 ---ITFASTEPRFFQKSLLKFYEFSPWFSFPNNIANASILQVLAEEPNNLRTLHILDIGV 300

Query: 301 SHGVQWPTLLEALTRRSGGPPPLIRLTVI--APSIEHDENIETPFSIGPPGDNIFSRLLS 360
           SHGVQWPT LEAL+RR GGPPPL+RLTV+  + S E+D+N+ETPFSIGP GD   S LL 
Sbjct: 301 SHGVQWPTFLEALSRRPGGPPPLVRLTVVNASSSTENDQNMETPFSIGPCGDTFSSGLLG 360

Query: 361 FAKSLNINLQINRLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQN 420
           +A+SLN+NLQI +LD+H LQ+LN++ +    DE LIVCAQFRLH L H  PDER EFL+ 
Sbjct: 361 YAQSLNVNLQIKKLDNHPLQTLNAKSVDTSSDETLIVCAQFRLHHLNHNNPDERSEFLKV 420

Query: 421 LREMEPKAVILSENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVME 480
           LR MEPK VILSENNM C C++CG+F TGF+RRVEYLWRFLDSTSSAFK R+SDER++ME
Sbjct: 421 LRGMEPKGVILSENNMECCCSSCGDFATGFSRRVEYLWRFLDSTSSAFKNRDSDERKMME 480

Query: 481 GEAAKALTNQGEMNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEK 540
           GEAAKALTNQ EMNE +EKW ERM+ AGF  + F ED ID  RA +R+YDNNWEM++EE 
Sbjct: 481 GEAAKALTNQREMNERREKWCERMKEAGFAGEVFGEDAIDGGRALLRKYDNNWEMKVEEN 540

Query: 541 DGCVGLWWKGQPVSFCSFWKL 548
              V LWWK QPVSFCS WKL
Sbjct: 541 STSVELWWKSQPVSFCSLWKL 549

BLAST of Tan0007499.1 vs. ExPASy Swiss-Prot
Match: A1DQP9 (Protein NODULATION SIGNALING PATHWAY 1 OS=Lotus japonicus OX=34305 GN=NSP1 PE=2 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 2.0e-180
Identity = 347/562 (61.74%), Postives = 410/562 (72.95%), Query Frame = 0

Query: 1   MTIEEPGPNH-PSDHILDWLEDSVPFFSPFLDETYNSSS-INCYQWWDE-----NQDIGQ 60
           MT+E   PN   SDHILDWLE SV FF  FLDE  N+S  I  Y  WD+     N     
Sbjct: 1   MTME---PNSTTSDHILDWLEGSVSFFPSFLDEPCNNSGYIQEYHLWDQYQTDANTGSSP 60

Query: 61  DLINGCLSGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQ 120
           +  N   +    T  T TT L    ++++   SDL KKR A D++  K   P QN+ K  
Sbjct: 61  NATNSTTATIVATSATSTTSLEPCGSNNNQPLSDLPKKRNATDESSLK---PPQNKNKRI 120

Query: 121 NNQSKNGADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANA 180
             +  N  +  NGD  R         NKK  +K+ G+N N+G++KEGRWAEQLLNPCA A
Sbjct: 121 KTRPMN--EPENGDAVR--------KNKKGGAKANGSNCNSGNSKEGRWAEQLLNPCAAA 180

Query: 181 IIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPVT 240
           I  G+  RV HLL VL ELASPTGD NHRLAAHGLRAL HHLSS+SSS +SS +    +T
Sbjct: 181 IAGGNVNRVQHLLYVLHELASPTGDPNHRLAAHGLRALTHHLSSSSSSPTSSGT----IT 240

Query: 241 FASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPN-RSRNLHILDIGVSHGV 300
           FAST+PRFFQ+SL+KF+EVSPWF+FPNNIAN+SIL  L+EE N  SR LHILDIGVSHGV
Sbjct: 241 FASTEPRFFQKSLLKFYEVSPWFSFPNNIANASILQVLAEEANITSRTLHILDIGVSHGV 300

Query: 301 QWPTLLEALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLN 360
           QWPTLL+AL+RRSGGPP ++RLTV+  + E+D+N+ETPFS  PPG N + RLL +A+S+N
Sbjct: 301 QWPTLLDALSRRSGGPPSVVRLTVV--TAENDQNMETPFSKAPPGYNYYPRLLGYAQSIN 360

Query: 361 INLQINRLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEP 420
           INLQINR+++HSLQ+LN+Q I   PDEILIVCAQFRLH L H +PDER EFL+ LR MEP
Sbjct: 361 INLQINRIENHSLQTLNAQSISASPDEILIVCAQFRLHHLNHNSPDERSEFLKVLRNMEP 420

Query: 421 KAVILSENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKA 480
           + VILSENN  C C+ CGNF  GFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKA
Sbjct: 421 RGVILSENNTECCCSGCGNFAAGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKA 480

Query: 481 LTNQGEMNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGL 540
           LTNQ EMNEEKEKW  RM+ AGF  + F ED +D  RA +R+YD+NWEM++EEK+  VGL
Sbjct: 481 LTNQREMNEEKEKWCGRMKEAGFAGEVFGEDAVDGGRALLRKYDSNWEMKVEEKNTSVGL 540

Query: 541 WWKGQPVSFCSFWKLGMKSNGG 555
           WWKGQPVSFCS WKL     GG
Sbjct: 541 WWKGQPVSFCSLWKLDGNDQGG 540

BLAST of Tan0007499.1 vs. ExPASy Swiss-Prot
Match: Q9LRW3 (Scarecrow-like protein 29 OS=Arabidopsis thaliana OX=3702 GN=SCL29 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 8.1e-129
Identity = 272/554 (49.10%), Postives = 349/554 (63.00%), Query Frame = 0

Query: 4   EEPGPNHPSDHILDWLEDSVPFFS-PFLDETYNSSSINCYQ-W-WDENQD--------IG 63
           E   PN   DH+L WLEDSV     P  D++Y     +  Q W WD+ QD          
Sbjct: 5   ETEPPNQTLDHVLSWLEDSVSLSPLPGFDDSYLLHEFDGSQTWEWDQTQDPEHGFIQSYS 64

Query: 64  QDLINGCLSGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKN 123
           QDL +    G   T   V T  P+I         DL  + + P+D   K+S         
Sbjct: 65  QDL-SAAYVGCEATNLEVVTEAPSIDL-------DLPPEIQQPNDQSRKRSH-------- 124

Query: 124 QNNQSKNGADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCAN 183
                       +G +E    +KK+  +K+ + KS+  +  +G NKEGRWAE+LLNPCA 
Sbjct: 125 ------------DGFLE-AQQVKKSARSKRKAIKSSEKSSKDG-NKEGRWAEKLLNPCAL 184

Query: 184 AIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPV 243
           AI   +++RV H LCVL ELAS +GDAN RLAA GLRAL HHL    SSSS SSS     
Sbjct: 185 AITASNSSRVQHYLCVLSELASSSGDANRRLAAFGLRALQHHL----SSSSVSSSFWPVF 244

Query: 244 TFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGV 303
           TFAS + + FQ++L+KF+EVSPWFA PNN+ANS+IL  L+++P   ++LHI+DIGVSHG+
Sbjct: 245 TFASAEVKMFQKTLLKFYEVSPWFALPNNMANSAILQILAQDPKDKKDLHIIDIGVSHGM 304

Query: 304 QWPTLLEALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLN 363
           QWPTLLEAL+ R  GPPP +R+TVI+     D   + PFS+GPPG N  S+LL FA+SL 
Sbjct: 305 QWPTLLEALSCRLEGPPPRVRITVIS-----DLTADIPFSVGPPGYNYGSQLLGFARSLK 364

Query: 364 INLQINRLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEP 423
           INLQI+ LD         Q+I   P E LIVCAQFRLH LKH   DER E L+ +R + P
Sbjct: 365 INLQISVLD-------KLQLIDTSPHENLIVCAQFRLHHLKHSINDERGETLKAVRSLRP 424

Query: 424 KAVILSENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKA 483
           K V+L ENN  CS  +  +F  GF++++EY+W+FLDSTSS FK   S+ER++MEGEA K 
Sbjct: 425 KGVVLCENNGECS--SSADFAAGFSKKLEYVWKFLDSTSSGFKEENSEERKLMEGEATKV 484

Query: 484 LTNQGEMNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGL 543
           L N G+MNE KEKWYERMR AGF  + F ED +D A++ +R+YDNNWE+R+E+ D   GL
Sbjct: 485 LMNAGDMNEGKEKWYERMREAGFFVEAFEEDAVDGAKSLLRKYDNNWEIRMEDGDTFAGL 510

Query: 544 WWKGQPVSFCSFWK 547
            WKG+ VSFCS WK
Sbjct: 545 MWKGEAVSFCSLWK 510

BLAST of Tan0007499.1 vs. ExPASy Swiss-Prot
Match: Q84MQ9 (Protein NODULATION SIGNALING PATHWAY 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP1 PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.1e-84
Identity = 209/523 (39.96%), Postives = 287/523 (54.88%), Query Frame = 0

Query: 44  WWDENQDIGQDLINGCLSG--SPTTVTTVTTRLPNIPTSHHLTPSDL----TKKRKAPDD 103
           WW  +    QD I   ++   SP +        P+I +    +PSD+    +KKRK+P  
Sbjct: 46  WWAASPAAQQDDIGSVVAQTLSPPSTAAPAAASPSIASPAASSPSDVPSSSSKKRKSP-- 105

Query: 104 TVHKKSQPHQNQRKNQNNQSKNGADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSN 163
                     ++        K G  KG G                            GS+
Sbjct: 106 ---------AHRAPGHTGGKKGGGGKGGG---------------------------GGSD 165

Query: 164 KEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSS 223
           ++ RWAEQLLNPCA A+  G+ +RV HL  VL EL S +GDANHRLAAHGLRALA  L +
Sbjct: 166 RDMRWAEQLLNPCAVAVEAGNLSRVQHLFYVLGELESFSGDANHRLAAHGLRALARWLPA 225

Query: 224 NSSSSSSSSSTVAP------VTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILH-- 283
               +++++  V P        FA+ +PR F+ SLI+FHEVSPWFA PN +AN++I    
Sbjct: 226 AVGPAAAAAVRVPPCSERPTTAFAAAEPRLFRASLIRFHEVSPWFALPNALANAAIAQAS 285

Query: 284 TLSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGG-PPPLIRLTVIAPSIEHDENIE 343
           T        R LH++D+GVSHGVQWPTLLE+LTR+ GG  PP +RLTV+ P       + 
Sbjct: 286 TCGAAGATPRPLHVVDLGVSHGVQWPTLLESLTRQPGGRAPPSVRLTVVGPGATATSPV- 345

Query: 344 TPFSIGPPGDNIFSRLLSFAKSLNINLQINRLDSHSLQSLNSQVIGKFPDEILIVCAQFR 403
            PFS  PPG +    LL +AKS+N++L+I+R       +L+  V G    E L+VC QFR
Sbjct: 346 APFSASPPGYDFSPHLLRYAKSINLDLRISR-----AATLDDAVPGD-DGEALVVCLQFR 405

Query: 404 LHQLKHYAPDERFEFLQNLREMEPKAVILSE--NNMACSCNNCGNFDTGFTRRVEYLWRF 463
              L H A +ER E L+  R + P+ V+LSE  + +     + G+    F  R+E LWRF
Sbjct: 406 ---LGHAAAEERREVLRKARGLNPELVVLSELDSGVGVVGGDGGSAAGEFAARLELLWRF 465

Query: 464 LDSTSSAFKGRESDERRVMEGEAAK--ALTNQGEMNEEKEKWYERMRNAGFVRKFFAEDT 523
           L+STS+AFKG++ +ERR++E EA    A  +     E +E W ERM  AGF    F  + 
Sbjct: 466 LESTSAAFKGKDVEERRLLEAEAGAILAAADVAAAGEGREGWRERMAAAGFEEAPFGAEA 520

Query: 524 IDTARASMRRYDNNWEMRI-EEKDGCVGLWWKGQPVSFCSFWK 547
           +++AR+ +R+YD+ WEM         V L WKGQPVSFCS W+
Sbjct: 526 VESARSLLRKYDSGWEMSAPSPAAAAVALRWKGQPVSFCSLWR 520

BLAST of Tan0007499.1 vs. ExPASy Swiss-Prot
Match: Q9SN22 (Scarecrow-like protein 32 OS=Arabidopsis thaliana OX=3702 GN=SCL32 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.7e-36
Identity = 124/409 (30.32%), Postives = 191/409 (46.70%), Query Frame = 0

Query: 159 EGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSN 218
           +  + EQLL  CA AI   DA   H +L VL  +A P GD+  RL +  LRAL   LS  
Sbjct: 23  DANFMEQLLLHCATAIDSNDAALTHQILWVLNNIAPPDGDSTQRLTSAFLRAL---LSRA 82

Query: 219 SSSSSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRS 278
            S + + SST++ +  A    RF    L  F +++PW  F    AN++IL  +       
Sbjct: 83  VSKTPTLSSTISFLPQADELHRFSVVELAAFVDLTPWHRFGFIAANAAILTAV----EGY 142

Query: 279 RNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGD 338
             +HI+D+ ++H +Q PTL++A+  R   PPPL++LTV++ S      I   +      +
Sbjct: 143 STVHIVDLSLTHCMQIPTLIDAMASRLNKPPPLLKLTVVSSSDHFPPFINISY------E 202

Query: 339 NIFSRLLSFAKSLNINLQINRLDSHSLQSLNS--QVIGKFP---DEILIVCAQFRLHQLK 398
            + S+L++FA + NI ++   + S      +S  Q +  +P   +E L+V      H + 
Sbjct: 203 ELGSKLVNFATTRNITMEFTIVPSTYSDGFSSLLQQLRIYPSSFNEALVV----NCHMML 262

Query: 399 HYAPDE---------RFEFLQNLREMEPKAVILSENNMACSCNNCGNFDTGFTRRVEYLW 458
            Y P+E         R  FL+ LR + P+ V L E ++  +  N  N          Y W
Sbjct: 263 RYIPEEPLTSSSSSLRTVFLKQLRSLNPRIVTLIEEDVDLTSENLVN---RLKSAFNYFW 322

Query: 459 RFLDSTSSAFKGRESDERRVMEGE--------AAKALTNQGEMNEEKEKWYERMRNAGFV 518
              D+T +      S++RR  E E         AK    + E  E K +W ERMR A F 
Sbjct: 323 IPFDTTDTFM----SEQRRWYEAEISWKIENVVAKEGAERVERTETKRRWIERMREAEFG 382

Query: 519 RKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFW 546
                ED +   +A +  +   W M+ E+ D  + L WKG  V F + W
Sbjct: 383 GVRVKEDAVADVKAMLEEHAVGWGMKKEDDDESLVLTWKGHSVVFATVW 407

BLAST of Tan0007499.1 vs. NCBI nr
Match: XP_023000031.1 (nodulation-signaling pathway 1 protein [Cucurbita maxima])

HSP 1 Score: 999.6 bits (2583), Expect = 1.1e-287
Identity = 500/555 (90.09%), Postives = 522/555 (94.05%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFF-SPFLDETYNSSSINCYQWWDENQDIGQDLINGC 60
           MTIEEPG NHPSDHILDWL DSVPFF SPF D++YNSSSINCYQWWDENQDIGQDLINGC
Sbjct: 40  MTIEEPGANHPSDHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGC 99

Query: 61  LSGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKN 120
           LS SP   TTV+T+ PN  TSHHLTPSDLTKKRKAPDDTVHK SQ  QNQRKNQNNQSKN
Sbjct: 100 LSSSP---TTVSTKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKN 159

Query: 121 GADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDA 180
           GADKG+G VE VTVIKK+VGNK+NSSK+TGNN +NGSNKEGRWAEQLLNPCANAIIKGDA
Sbjct: 160 GADKGSGAVEGVTVIKKSVGNKRNSSKATGNNNHNGSNKEGRWAEQLLNPCANAIIKGDA 219

Query: 181 TRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPVTFASTDP 240
           TRVHHLLCVLQELASPTGDANHRLAA+GLRALAH+LSSN SS  SSSSTVAPVTFASTD 
Sbjct: 220 TRVHHLLCVLQELASPTGDANHRLAAYGLRALAHYLSSN-SSCYSSSSTVAPVTFASTDL 279

Query: 241 RFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFAFPNNIANSSILH LSEEPNRSRNLHILDIGVSHGVQWPTLLE
Sbjct: 280 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLE 339

Query: 301 ALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTVIAP++EHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQINR
Sbjct: 340 ALTRRSGGPPPLIRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 399

Query: 361 LDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSE 420
           LD+ SLQS NSQVIGK PDEILIVCAQFRLHQLKHYAPDERFEFLQNLR++EPKAVILSE
Sbjct: 400 LDNRSLQSFNSQVIGKLPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSE 459

Query: 421 NNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480
           NNMACSCNNCGNFDTGFTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAA+ LTNQGEM
Sbjct: 460 NNMACSCNNCGNFDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAARGLTNQGEM 519

Query: 481 NEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPV 540
           NEE EKW ERMRNAGF RK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPV
Sbjct: 520 NEEMEKWCERMRNAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPV 579

Query: 541 SFCSFWKLGMKSNGG 555
           SFCSFWKLGMKSNGG
Sbjct: 580 SFCSFWKLGMKSNGG 590

BLAST of Tan0007499.1 vs. NCBI nr
Match: XP_038897214.1 (protein NODULATION SIGNALING PATHWAY 1 [Benincasa hispida])

HSP 1 Score: 983.0 bits (2540), Expect = 1.0e-282
Identity = 496/554 (89.53%), Postives = 515/554 (92.96%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLDETYNSSSINCYQWWDENQDIGQDLINGCL 60
           MTIEE GPNHPSDHILDWLEDSVPFFSPFLDET NSSSINCYQWWD NQD G+DLING L
Sbjct: 1   MTIEETGPNHPSDHILDWLEDSVPFFSPFLDETNNSSSINCYQWWDANQDTGEDLINGFL 60

Query: 61  SGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNG 120
           S SP   TTV+T+L NIPTSHHLTPSDLTKKRKAPDD+VHKKSQ HQN+RKNQNNQSKNG
Sbjct: 61  SNSP---TTVSTKLQNIPTSHHLTPSDLTKKRKAPDDSVHKKSQTHQNRRKNQNNQSKNG 120

Query: 121 ADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
              G G VE VTV+KK+VGNKKNSSK TGNN NNGSN+EGRWAEQLLNPCA+AIIKGDAT
Sbjct: 121 ---GGGAVEGVTVMKKSVGNKKNSSKFTGNNCNNGSNREGRWAEQLLNPCASAIIKGDAT 180

Query: 181 RVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSS--SSSSSTVAPVTFASTD 240
           RVHHLLCVLQELASPTGDANHRLA HGLRALAHHLSSNSSSS  SS SSTVAPVTFASTD
Sbjct: 181 RVHHLLCVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSFSSYSSTVAPVTFASTD 240

Query: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLL 300
           PRFFQRSLIKFHEVSPWFAFPNNIANSSILH LSEEPNR RNLHILDIGVSHGVQWPTLL
Sbjct: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRPRNLHILDIGVSHGVQWPTLL 300

Query: 301 EALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQIN 360
           EALTRRSGGPP LIRLTVI P+IEHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQIN
Sbjct: 301 EALTRRSGGPPSLIRLTVIGPTIEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 360

Query: 361 RLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILS 420
           RL++HSLQSLNSQVI KFPDEILIVCAQFRLHQLKH  PDERFEFLQNLR+MEPKAVILS
Sbjct: 361 RLNNHSLQSLNSQVINKFPDEILIVCAQFRLHQLKHCDPDERFEFLQNLRKMEPKAVILS 420

Query: 421 ENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGE 480
           ENNM CSC+NCGNFDT FTRRVEYLWRFLDSTS+AFKGRES+ERRVMEGEAAKALTN GE
Sbjct: 421 ENNMGCSCSNCGNFDTRFTRRVEYLWRFLDSTSAAFKGRESEERRVMEGEAAKALTNDGE 480

Query: 481 MNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQP 540
           MNEEK KW ERMRNAGF RK FAEDTIDTARASMRRYDNNWEMRIEEKDGC+GLWWKGQP
Sbjct: 481 MNEEKGKWCERMRNAGFERKLFAEDTIDTARASMRRYDNNWEMRIEEKDGCLGLWWKGQP 540

Query: 541 VSFCSFWKLGMKSN 553
           VSFCSFWKLG+KSN
Sbjct: 541 VSFCSFWKLGIKSN 548

BLAST of Tan0007499.1 vs. NCBI nr
Match: XP_023514155.1 (nodulation-signaling pathway 1 protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976.1 bits (2522), Expect = 1.3e-280
Identity = 488/543 (89.87%), Postives = 513/543 (94.48%), Query Frame = 0

Query: 13  DHILDWLEDSVPFF-SPFLDETYNSSSINCYQWWDENQDIGQDLINGCLSGSPTTVTTVT 72
           DHILDWL DSVPFF SPF D++YNSSSINCYQWWDENQDIGQDLINGCLS SP   TTV+
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNGADKGNGDVERV 132
           T+ PN  TSHHLTPSDLTKKRKAPDDTVHK SQ  QNQRKNQNNQSKNGADK +G V  V
Sbjct: 104 TKPPNNSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKNGADKSSGAVAGV 163

Query: 133 TVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 192
           TV+KK+VGNK+NSSK+TG+N NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGSNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPVTFASTDPRFFQRSLIKFHE 252
           LASPTGDANHRLAA+GLRALAH+LSSN SS SSSSST+APVTFASTDPRFFQRSLIKFHE
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSN-SSCSSSSSTLAPVTFASTDPRFFQRSLIKFHE 283

Query: 253 VSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFAFPNNIANSSILH LSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 284 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 343

Query: 313 IRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINRLDSHSLQSLNSQ 372
           IRLTVIAP++EHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQINRLD+ SLQSLNSQ
Sbjct: 344 IRLTVIAPTLEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNRSLQSLNSQ 403

Query: 373 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSENNMACSCNNCGN 432
           VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLR+MEPKAVILSENNMACSCNNCGN
Sbjct: 404 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKMEPKAVILSENNMACSCNNCGN 463

Query: 433 FDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERMR 492
           FDTGFTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAAKAL N+GEMNEE EKW ERMR
Sbjct: 464 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAAKALRNEGEMNEEMEKWCERMR 523

Query: 493 NAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKLGMKS 552
           NAGF RK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPVSFCSFWKLG+KS
Sbjct: 524 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGIKS 582

Query: 553 NGG 555
           NGG
Sbjct: 584 NGG 582

BLAST of Tan0007499.1 vs. NCBI nr
Match: XP_022148342.1 (nodulation-signaling pathway 1 protein [Momordica charantia])

HSP 1 Score: 969.1 bits (2504), Expect = 1.6e-278
Identity = 486/555 (87.57%), Postives = 509/555 (91.71%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLDETYNSSSINCYQWWDENQDIGQDLINGCL 60
           MTIEEPGPNHPSDHILDWLEDS PFFSPFLDETYNSSSINCYQWWDE+Q+IGQDLINGCL
Sbjct: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60

Query: 61  SGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNG 120
           S SP   TT +T  PN  +   LTPSDL+KKRKAPDDT HK +QPHQN RKNQNNQSKNG
Sbjct: 61  SSSPAAATTDSTTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 120

Query: 121 ADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG G      V+KK+VGNKK+SSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT
Sbjct: 121 ADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180

Query: 181 RVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAP-VTFASTDP 240
           RVHHL+CVLQELASPTGDANHRLA HGLRALAHHLSSN   SSSSSST+AP V FASTD 
Sbjct: 181 RVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSN---SSSSSSTLAPVVNFASTDA 240

Query: 241 RFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFA PNNIANSSILHTLSEEPN SRNLHILDIGVSHGVQWPTLLE
Sbjct: 241 RFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLE 300

Query: 301 ALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTV+AP++EHD+  ETPFSIGPPGDNI SRLLSFAKSLNINLQINR
Sbjct: 301 ALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360

Query: 361 LDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSE 420
           LD+HSLQ+LNSQVIGKF DEILIVCA FRLHQLKH APDER EFL+NLR+MEP AVILSE
Sbjct: 361 LDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSE 420

Query: 421 NNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480
           NN+ACSC+NCGNFD  FTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM
Sbjct: 421 NNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480

Query: 481 NEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPV 540
           NEEKEKWYERMRNAGF RKFFAE TIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQP+
Sbjct: 481 NEEKEKWYERMRNAGFARKFFAEXTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPI 540

Query: 541 SFCSFWKLGMKSNGG 555
           SFCSFWKLG K NGG
Sbjct: 541 SFCSFWKLGAKPNGG 544

BLAST of Tan0007499.1 vs. NCBI nr
Match: XP_022964298.1 (nodulation-signaling pathway 1 protein [Cucurbita moschata])

HSP 1 Score: 969.1 bits (2504), Expect = 1.6e-278
Identity = 484/543 (89.13%), Postives = 510/543 (93.92%), Query Frame = 0

Query: 13  DHILDWLEDSVPFF-SPFLDETYNSSSINCYQWWDENQDIGQDLINGCLSGSPTTVTTVT 72
           DHILDWL DSVPFF SPF D++YNSSSINCYQWWDENQDIGQDLINGCLS SP   TTV+
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNGADKGNGDVERV 132
           T+ PN  TSHHLTPSDLTKKRKAPDDTVHK SQ  QNQRKNQNNQS+NGADK +G V  V
Sbjct: 104 TKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSRNGADKSSGAVAGV 163

Query: 133 TVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 192
           TV+KK+VGNK+NSSK+TGNN NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGNNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPVTFASTDPRFFQRSLIKFHE 252
           LASPTGDANHRLAA+GLRALAH+LSSN  SS SSSST+APVTFASTDPRFFQRSLIKFHE
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSN--SSCSSSSTLAPVTFASTDPRFFQRSLIKFHE 283

Query: 253 VSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFAFPNNIANSSILH LSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 284 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 343

Query: 313 IRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINRLDSHSLQSLNSQ 372
           IRLTVIAP++EHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQINRLDS SL SLN+Q
Sbjct: 344 IRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDSLSLLSLNAQ 403

Query: 373 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSENNMACSCNNCGN 432
           VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLR++EPKAVILSENNMACSC NCGN
Sbjct: 404 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSENNMACSCTNCGN 463

Query: 433 FDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERMR 492
           FDTGFTR+VEYLWRFLDSTSSAFKGRES+ER++MEGEAAKAL N+GEMNEE EKW ERMR
Sbjct: 464 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKLMEGEAAKALRNEGEMNEEMEKWCERMR 523

Query: 493 NAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKLGMKS 552
           NAGF RK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPVSFCSFWKLGMKS
Sbjct: 524 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGMKS 581

Query: 553 NGG 555
           NGG
Sbjct: 584 NGG 581

BLAST of Tan0007499.1 vs. ExPASy TrEMBL
Match: A0A6J1KER4 (nodulation-signaling pathway 1 protein OS=Cucurbita maxima OX=3661 GN=LOC111494340 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.2e-288
Identity = 500/555 (90.09%), Postives = 522/555 (94.05%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFF-SPFLDETYNSSSINCYQWWDENQDIGQDLINGC 60
           MTIEEPG NHPSDHILDWL DSVPFF SPF D++YNSSSINCYQWWDENQDIGQDLINGC
Sbjct: 40  MTIEEPGANHPSDHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGC 99

Query: 61  LSGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKN 120
           LS SP   TTV+T+ PN  TSHHLTPSDLTKKRKAPDDTVHK SQ  QNQRKNQNNQSKN
Sbjct: 100 LSSSP---TTVSTKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKN 159

Query: 121 GADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDA 180
           GADKG+G VE VTVIKK+VGNK+NSSK+TGNN +NGSNKEGRWAEQLLNPCANAIIKGDA
Sbjct: 160 GADKGSGAVEGVTVIKKSVGNKRNSSKATGNNNHNGSNKEGRWAEQLLNPCANAIIKGDA 219

Query: 181 TRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPVTFASTDP 240
           TRVHHLLCVLQELASPTGDANHRLAA+GLRALAH+LSSN SS  SSSSTVAPVTFASTD 
Sbjct: 220 TRVHHLLCVLQELASPTGDANHRLAAYGLRALAHYLSSN-SSCYSSSSTVAPVTFASTDL 279

Query: 241 RFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFAFPNNIANSSILH LSEEPNRSRNLHILDIGVSHGVQWPTLLE
Sbjct: 280 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLE 339

Query: 301 ALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTVIAP++EHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQINR
Sbjct: 340 ALTRRSGGPPPLIRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 399

Query: 361 LDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSE 420
           LD+ SLQS NSQVIGK PDEILIVCAQFRLHQLKHYAPDERFEFLQNLR++EPKAVILSE
Sbjct: 400 LDNRSLQSFNSQVIGKLPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSE 459

Query: 421 NNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480
           NNMACSCNNCGNFDTGFTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAA+ LTNQGEM
Sbjct: 460 NNMACSCNNCGNFDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAARGLTNQGEM 519

Query: 481 NEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPV 540
           NEE EKW ERMRNAGF RK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPV
Sbjct: 520 NEEMEKWCERMRNAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPV 579

Query: 541 SFCSFWKLGMKSNGG 555
           SFCSFWKLGMKSNGG
Sbjct: 580 SFCSFWKLGMKSNGG 590

BLAST of Tan0007499.1 vs. ExPASy TrEMBL
Match: A0A6J1D4T6 (nodulation-signaling pathway 1 protein OS=Momordica charantia OX=3673 GN=LOC111017018 PE=3 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 7.5e-279
Identity = 486/555 (87.57%), Postives = 509/555 (91.71%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLDETYNSSSINCYQWWDENQDIGQDLINGCL 60
           MTIEEPGPNHPSDHILDWLEDS PFFSPFLDETYNSSSINCYQWWDE+Q+IGQDLINGCL
Sbjct: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60

Query: 61  SGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNG 120
           S SP   TT +T  PN  +   LTPSDL+KKRKAPDDT HK +QPHQN RKNQNNQSKNG
Sbjct: 61  SSSPAAATTDSTTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 120

Query: 121 ADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG G      V+KK+VGNKK+SSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT
Sbjct: 121 ADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180

Query: 181 RVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAP-VTFASTDP 240
           RVHHL+CVLQELASPTGDANHRLA HGLRALAHHLSSN   SSSSSST+AP V FASTD 
Sbjct: 181 RVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSN---SSSSSSTLAPVVNFASTDA 240

Query: 241 RFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFA PNNIANSSILHTLSEEPN SRNLHILDIGVSHGVQWPTLLE
Sbjct: 241 RFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLE 300

Query: 301 ALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTV+AP++EHD+  ETPFSIGPPGDNI SRLLSFAKSLNINLQINR
Sbjct: 301 ALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360

Query: 361 LDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSE 420
           LD+HSLQ+LNSQVIGKF DEILIVCA FRLHQLKH APDER EFL+NLR+MEP AVILSE
Sbjct: 361 LDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSE 420

Query: 421 NNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480
           NN+ACSC+NCGNFD  FTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM
Sbjct: 421 NNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480

Query: 481 NEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPV 540
           NEEKEKWYERMRNAGF RKFFAE TIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQP+
Sbjct: 481 NEEKEKWYERMRNAGFARKFFAEXTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPI 540

Query: 541 SFCSFWKLGMKSNGG 555
           SFCSFWKLG K NGG
Sbjct: 541 SFCSFWKLGAKPNGG 544

BLAST of Tan0007499.1 vs. ExPASy TrEMBL
Match: A0A6J1HKE2 (nodulation-signaling pathway 1 protein OS=Cucurbita moschata OX=3662 GN=LOC111464355 PE=3 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 7.5e-279
Identity = 484/543 (89.13%), Postives = 510/543 (93.92%), Query Frame = 0

Query: 13  DHILDWLEDSVPFF-SPFLDETYNSSSINCYQWWDENQDIGQDLINGCLSGSPTTVTTVT 72
           DHILDWL DSVPFF SPF D++YNSSSINCYQWWDENQDIGQDLINGCLS SP   TTV+
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNGADKGNGDVERV 132
           T+ PN  TSHHLTPSDLTKKRKAPDDTVHK SQ  QNQRKNQNNQS+NGADK +G V  V
Sbjct: 104 TKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSRNGADKSSGAVAGV 163

Query: 133 TVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 192
           TV+KK+VGNK+NSSK+TGNN NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGNNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPVTFASTDPRFFQRSLIKFHE 252
           LASPTGDANHRLAA+GLRALAH+LSSN  SS SSSST+APVTFASTDPRFFQRSLIKFHE
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSN--SSCSSSSTLAPVTFASTDPRFFQRSLIKFHE 283

Query: 253 VSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFAFPNNIANSSILH LSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 284 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 343

Query: 313 IRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINRLDSHSLQSLNSQ 372
           IRLTVIAP++EHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQINRLDS SL SLN+Q
Sbjct: 344 IRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDSLSLLSLNAQ 403

Query: 373 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSENNMACSCNNCGN 432
           VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLR++EPKAVILSENNMACSC NCGN
Sbjct: 404 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSENNMACSCTNCGN 463

Query: 433 FDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERMR 492
           FDTGFTR+VEYLWRFLDSTSSAFKGRES+ER++MEGEAAKAL N+GEMNEE EKW ERMR
Sbjct: 464 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKLMEGEAAKALRNEGEMNEEMEKWCERMR 523

Query: 493 NAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKLGMKS 552
           NAGF RK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPVSFCSFWKLGMKS
Sbjct: 524 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGMKS 581

Query: 553 NGG 555
           NGG
Sbjct: 584 NGG 581

BLAST of Tan0007499.1 vs. ExPASy TrEMBL
Match: A0A0A0KCK6 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450500 PE=3 SV=1)

HSP 1 Score: 963.4 bits (2489), Expect = 4.1e-277
Identity = 486/555 (87.57%), Postives = 509/555 (91.71%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLDETYNSSSINCYQWWDENQDIGQDLINGCL 60
           MTIEE GPNHPSDHILDWLEDSVPFFS FLDET NSSSINCYQWWDENQD G+DLINGCL
Sbjct: 1   MTIEETGPNHPSDHILDWLEDSVPFFSSFLDETSNSSSINCYQWWDENQDTGEDLINGCL 60

Query: 61  SGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNG 120
           S SPTTV  V+TR PN PTSH LTPSDLTKKRKAPDD+VHKKSQ HQN RKNQNNQSKN 
Sbjct: 61  SNSPTTV--VSTRQPNTPTSHSLTPSDLTKKRKAPDDSVHKKSQTHQNPRKNQNNQSKNS 120

Query: 121 ADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG+G VE VTV+KK+VGNKKN+SKSTGNNYN+GSNKEGRWAEQLLNPCANAI+KGDAT
Sbjct: 121 ADKGSGAVEGVTVMKKSVGNKKNASKSTGNNYNSGSNKEGRWAEQLLNPCANAIVKGDAT 180

Query: 181 RVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSS--SSSSSTVAPVTFASTD 240
           RVHHLLCVLQELASPTGDANHRLA HGLRALA+HLSSNSSSS  SS SSTVAP TFASTD
Sbjct: 181 RVHHLLCVLQELASPTGDANHRLADHGLRALAYHLSSNSSSSSFSSYSSTVAPFTFASTD 240

Query: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLL 300
           PRFFQRSLIKFHEVSPWFAFPNNIANSSILH LSEE NR RNLHILDIGVSHGVQWPTLL
Sbjct: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEESNRPRNLHILDIGVSHGVQWPTLL 300

Query: 301 EALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQIN 360
           EALTRRSGGPPPLIRLTVIAP+IEHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQIN
Sbjct: 301 EALTRRSGGPPPLIRLTVIAPTIEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 360

Query: 361 RLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILS 420
           RLD HSLQSLNSQ I K  DEILIVCAQFRLHQLKH APDER EFL+NLR+MEPKAVILS
Sbjct: 361 RLDIHSLQSLNSQAINKSRDEILIVCAQFRLHQLKHSAPDERQEFLENLRKMEPKAVILS 420

Query: 421 ENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTN-QG 480
           ENNM CSC+ CGNF+ GF R VEY+W+FLDSTS+AFKGRES+ERRVMEGEAAKAL N  G
Sbjct: 421 ENNMGCSCSKCGNFEMGFRRGVEYVWKFLDSTSAAFKGRESEERRVMEGEAAKALRNVDG 480

Query: 481 EMNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQ 540
           EMNEEK KW ERMRN GF RK F EDTIDTARASMRRYDNNWEMR+E+KDGCVGLWWKGQ
Sbjct: 481 EMNEEKGKWCERMRNVGFERKRFGEDTIDTARASMRRYDNNWEMRMEDKDGCVGLWWKGQ 540

Query: 541 PVSFCSFWKLGMKSN 553
           PVSFCSFWKLG+KSN
Sbjct: 541 PVSFCSFWKLGIKSN 553

BLAST of Tan0007499.1 vs. ExPASy TrEMBL
Match: A0A1S3CGQ3 (nodulation-signaling pathway 1 protein OS=Cucumis melo OX=3656 GN=LOC103500695 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 1.0e-275
Identity = 482/555 (86.85%), Postives = 509/555 (91.71%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLDETYNSSSINCYQWWDENQDIGQDLINGCL 60
           MTIEE GP+HPSDHILDWLEDSVPFFS FLDET NSSSINCYQWWDENQD G+DLINGCL
Sbjct: 44  MTIEETGPDHPSDHILDWLEDSVPFFSSFLDETSNSSSINCYQWWDENQDTGEDLINGCL 103

Query: 61  SGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKNQNNQSKNG 120
           S SPTTV  V+TR PN PTSHHL PSDLTKKRKAPDD+VHKKSQ HQN RKNQNNQSKN 
Sbjct: 104 SNSPTTV--VSTRQPNTPTSHHLPPSDLTKKRKAPDDSVHKKSQTHQNPRKNQNNQSKNI 163

Query: 121 ADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG+G VE VTVIKK+VGNKKN+SKSTGNNYNNGSNKEGRWAEQLLNPCANAI+KGDAT
Sbjct: 164 ADKGSGAVEGVTVIKKSVGNKKNASKSTGNNYNNGSNKEGRWAEQLLNPCANAIVKGDAT 223

Query: 181 RVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSS--SSSSSTVAPVTFASTD 240
           RVHHLLCVLQELASPTGDANHRLA HGLRALA+HLSSNSSSS  SS SSTV+P+TFASTD
Sbjct: 224 RVHHLLCVLQELASPTGDANHRLADHGLRALAYHLSSNSSSSSFSSYSSTVSPITFASTD 283

Query: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLL 300
           PRFFQRSLIKFHEVSPWFAFPNNIANSSILH LSEE NR RNLH+LDIGVSHGVQWPTLL
Sbjct: 284 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEESNRPRNLHVLDIGVSHGVQWPTLL 343

Query: 301 EALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQIN 360
           EALTRRSGGPPPLIRLTVIAP++EHD+N ETPFSIGPPGDNI SRLLSFAKSLNINLQIN
Sbjct: 344 EALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 403

Query: 361 RLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILS 420
           RLD HSLQSLNSQ I K  DEILIVC+QFRLHQLKH APDER EFLQNLR+MEPKAVILS
Sbjct: 404 RLDIHSLQSLNSQAINKSRDEILIVCSQFRLHQLKHSAPDERQEFLQNLRKMEPKAVILS 463

Query: 421 ENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTN-QG 480
           ENNM CSC+ C NF+ GF R VEY+W+FLDSTS+AFKGRES+ERRVMEGEAAKAL N +G
Sbjct: 464 ENNMGCSCSKCVNFEMGFRRGVEYVWKFLDSTSAAFKGRESEERRVMEGEAAKALRNVEG 523

Query: 481 EMNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQ 540
           EMNEEK KW ERMRN GF RK F EDTIDTARASMRRYDNNWEMR+E+KDGCVGLWWKGQ
Sbjct: 524 EMNEEKGKWCERMRNVGFERKRFGEDTIDTARASMRRYDNNWEMRMEDKDGCVGLWWKGQ 583

Query: 541 PVSFCSFWKLGMKSN 553
           PVSFCS WKLG+KSN
Sbjct: 584 PVSFCSLWKLGIKSN 596

BLAST of Tan0007499.1 vs. TAIR 10
Match: AT3G13840.1 (GRAS family transcription factor )

HSP 1 Score: 462.2 bits (1188), Expect = 5.8e-130
Identity = 272/554 (49.10%), Postives = 349/554 (63.00%), Query Frame = 0

Query: 4   EEPGPNHPSDHILDWLEDSVPFFS-PFLDETYNSSSINCYQ-W-WDENQD--------IG 63
           E   PN   DH+L WLEDSV     P  D++Y     +  Q W WD+ QD          
Sbjct: 5   ETEPPNQTLDHVLSWLEDSVSLSPLPGFDDSYLLHEFDGSQTWEWDQTQDPEHGFIQSYS 64

Query: 64  QDLINGCLSGSPTTVTTVTTRLPNIPTSHHLTPSDLTKKRKAPDDTVHKKSQPHQNQRKN 123
           QDL +    G   T   V T  P+I         DL  + + P+D   K+S         
Sbjct: 65  QDL-SAAYVGCEATNLEVVTEAPSIDL-------DLPPEIQQPNDQSRKRSH-------- 124

Query: 124 QNNQSKNGADKGNGDVERVTVIKKAVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCAN 183
                       +G +E    +KK+  +K+ + KS+  +  +G NKEGRWAE+LLNPCA 
Sbjct: 125 ------------DGFLE-AQQVKKSARSKRKAIKSSEKSSKDG-NKEGRWAEKLLNPCAL 184

Query: 184 AIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTVAPV 243
           AI   +++RV H LCVL ELAS +GDAN RLAA GLRAL HHL    SSSS SSS     
Sbjct: 185 AITASNSSRVQHYLCVLSELASSSGDANRRLAAFGLRALQHHL----SSSSVSSSFWPVF 244

Query: 244 TFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVSHGV 303
           TFAS + + FQ++L+KF+EVSPWFA PNN+ANS+IL  L+++P   ++LHI+DIGVSHG+
Sbjct: 245 TFASAEVKMFQKTLLKFYEVSPWFALPNNMANSAILQILAQDPKDKKDLHIIDIGVSHGM 304

Query: 304 QWPTLLEALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGDNIFSRLLSFAKSLN 363
           QWPTLLEAL+ R  GPPP +R+TVI+     D   + PFS+GPPG N  S+LL FA+SL 
Sbjct: 305 QWPTLLEALSCRLEGPPPRVRITVIS-----DLTADIPFSVGPPGYNYGSQLLGFARSLK 364

Query: 364 INLQINRLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLREMEP 423
           INLQI+ LD         Q+I   P E LIVCAQFRLH LKH   DER E L+ +R + P
Sbjct: 365 INLQISVLD-------KLQLIDTSPHENLIVCAQFRLHHLKHSINDERGETLKAVRSLRP 424

Query: 424 KAVILSENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKA 483
           K V+L ENN  CS  +  +F  GF++++EY+W+FLDSTSS FK   S+ER++MEGEA K 
Sbjct: 425 KGVVLCENNGECS--SSADFAAGFSKKLEYVWKFLDSTSSGFKEENSEERKLMEGEATKV 484

Query: 484 LTNQGEMNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGL 543
           L N G+MNE KEKWYERMR AGF  + F ED +D A++ +R+YDNNWE+R+E+ D   GL
Sbjct: 485 LMNAGDMNEGKEKWYERMREAGFFVEAFEEDAVDGAKSLLRKYDNNWEIRMEDGDTFAGL 510

Query: 544 WWKGQPVSFCSFWK 547
            WKG+ VSFCS WK
Sbjct: 545 MWKGEAVSFCSLWK 510

BLAST of Tan0007499.1 vs. TAIR 10
Match: AT3G49950.1 (GRAS family transcription factor )

HSP 1 Score: 154.1 bits (388), Expect = 3.4e-37
Identity = 124/409 (30.32%), Postives = 191/409 (46.70%), Query Frame = 0

Query: 159 EGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSN 218
           +  + EQLL  CA AI   DA   H +L VL  +A P GD+  RL +  LRAL   LS  
Sbjct: 23  DANFMEQLLLHCATAIDSNDAALTHQILWVLNNIAPPDGDSTQRLTSAFLRAL---LSRA 82

Query: 219 SSSSSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRS 278
            S + + SST++ +  A    RF    L  F +++PW  F    AN++IL  +       
Sbjct: 83  VSKTPTLSSTISFLPQADELHRFSVVELAAFVDLTPWHRFGFIAANAAILTAV----EGY 142

Query: 279 RNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPPGD 338
             +HI+D+ ++H +Q PTL++A+  R   PPPL++LTV++ S      I   +      +
Sbjct: 143 STVHIVDLSLTHCMQIPTLIDAMASRLNKPPPLLKLTVVSSSDHFPPFINISY------E 202

Query: 339 NIFSRLLSFAKSLNINLQINRLDSHSLQSLNS--QVIGKFP---DEILIVCAQFRLHQLK 398
            + S+L++FA + NI ++   + S      +S  Q +  +P   +E L+V      H + 
Sbjct: 203 ELGSKLVNFATTRNITMEFTIVPSTYSDGFSSLLQQLRIYPSSFNEALVV----NCHMML 262

Query: 399 HYAPDE---------RFEFLQNLREMEPKAVILSENNMACSCNNCGNFDTGFTRRVEYLW 458
            Y P+E         R  FL+ LR + P+ V L E ++  +  N  N          Y W
Sbjct: 263 RYIPEEPLTSSSSSLRTVFLKQLRSLNPRIVTLIEEDVDLTSENLVN---RLKSAFNYFW 322

Query: 459 RFLDSTSSAFKGRESDERRVMEGE--------AAKALTNQGEMNEEKEKWYERMRNAGFV 518
              D+T +      S++RR  E E         AK    + E  E K +W ERMR A F 
Sbjct: 323 IPFDTTDTFM----SEQRRWYEAEISWKIENVVAKEGAERVERTETKRRWIERMREAEFG 382

Query: 519 RKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFW 546
                ED +   +A +  +   W M+ E+ D  + L WKG  V F + W
Sbjct: 383 GVRVKEDAVADVKAMLEEHAVGWGMKKEDDDESLVLTWKGHSVVFATVW 407

BLAST of Tan0007499.1 vs. TAIR 10
Match: AT4G37650.1 (GRAS family transcription factor )

HSP 1 Score: 142.9 bits (359), Expect = 7.7e-34
Identity = 115/405 (28.40%), Postives = 188/405 (46.42%), Query Frame = 0

Query: 161 RWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSS 220
           +WA+ +L   A A    D  R   +L  L EL+SP GD   +LA++ L+AL + ++ +  
Sbjct: 141 KWADSVLLEAARAFSDKDTARAQQILWTLNELSSPYGDTEQKLASYFLQALFNRMTGSGE 200

Query: 221 ----SSSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPN 280
               +  ++++T    +F ST     +++++KF EVSPW  F +  AN +IL  +  E  
Sbjct: 201 RCYRTMVTAAATEKTCSFEST-----RKTVLKFQEVSPWATFGHVAANGAILEAVDGE-- 260

Query: 281 RSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVIAPSIEHDENIETPFSIGPP 340
               +HI+DI  +   QWPTLLEAL  RS   P L   TV+  +   ++   +   +   
Sbjct: 261 --AKIHIVDISSTFCTQWPTLLEALATRSDDTPHLRLTTVVVANKFVNDQTASHRMMKEI 320

Query: 341 GDNI--FSRLLSFAKSLNINLQINRLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKH 400
           G+ +  F+RL+      NI   +  L    L  L+ +     PDE+L +     +H +  
Sbjct: 321 GNRMEKFARLMGVPFKFNIIHHVGDLSEFDLNELDVK-----PDEVLAINCVGAMHGIAS 380

Query: 401 YAPDERFEFLQNLREMEPKAVILSENNMACSCNNCGNFDTGFTRRVEYLWRF----LDST 460
                R   + + R + P+ V + E          G FD  F R      R+     +S 
Sbjct: 381 RG-SPRDAVISSFRRLRPRIVTVVEEEADLVGEEEGGFDDEFLRGFGECLRWFRVCFESW 440

Query: 461 SSAFKGRESDERRVMEGEAAKAL--------TNQGEMNEEKEKWYERMRNAGFVRKFFAE 520
             +F  R S+ER ++E  A +A+        ++  E  E   KW  RMRN+GF    +++
Sbjct: 441 EESFP-RTSNERLMLERAAGRAIVDLVACEPSDSTERRETARKWSRRMRNSGFGAVGYSD 500

Query: 521 DTIDTARASMRRY-DNNWEMRIEEKDGCVGLWWKGQPVSFCSFWK 547
           +  D  RA +RRY +  W M        + L W+ QPV + S W+
Sbjct: 501 EVADDVRALLRRYKEGVWSMVQCPDAAGIFLCWRDQPVVWASAWR 529

BLAST of Tan0007499.1 vs. TAIR 10
Match: AT5G66770.1 (GRAS family transcription factor )

HSP 1 Score: 117.9 bits (294), Expect = 2.7e-26
Identity = 112/392 (28.57%), Postives = 174/392 (44.39%), Query Frame = 0

Query: 170 CANAIIKGDATRVHHLLCVLQELASPTGDANHRLAAHGLRALAHHLSSNSSSSSSSSSTV 229
           CA  I   D       L  ++E  S  GD   R+A +   AL++ LS NS ++SSSSS+ 
Sbjct: 224 CAR-ISDSDPNEASKTLLQIRESVSELGDPTERVAFYFTEALSNRLSPNSPATSSSSSST 283

Query: 230 APVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHTLSEEPNRSRNLHILDIGVS 289
             +            S    ++  P+  F +  AN +IL    E   +S  +HI+D G+ 
Sbjct: 284 EDLIL----------SYKTLNDACPYSKFAHLTANQAIL----EATEKSNKIHIVDFGIV 343

Query: 290 HGVQWPTLLEALTRRSGGPPPLIRLTVI-APSIEHDENIETPFSIGPPGDNIFSRLLSFA 349
            G+QWP LL+AL  R+ G P  IR++ I APS+      E+P    P      +RL  FA
Sbjct: 344 QGIQWPALLQALATRTSGKPTQIRVSGIPAPSLG-----ESP---EPSLIATGNRLRDFA 403

Query: 350 KSLNINLQINRLDSHSLQSLNSQVIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLR 409
           K L++N     + +  +  LN       PDE+L V    +L++L    P      L+  +
Sbjct: 404 KVLDLNFDFIPILT-PIHLLNGSSFRVDPDEVLAVNFMLQLYKLLDETPTIVDTALRLAK 463

Query: 410 EMEPKAVILSENNMACSCNNCGNFDTGFTRRVEYLWRFLDSTSSAFK---GRESDER--- 469
            + P+ V L E  ++ +         GF  RV+   +F  +   + +   GR+S+ER   
Sbjct: 464 SLNPRVVTLGEYEVSLN-------RVGFANRVKNALQFYSAVFESLEPNLGRDSEERVRV 523

Query: 470 -RVMEGEAAKALTN------QGEMNEEKEKWYERMRNAGFVRKFFAEDTIDTARASMRRY 529
            R + G     L          E  EEKE+W   M NAGF     +   +  A+  +  Y
Sbjct: 524 ERELFGRRISGLIGPEKTGIHRERMEEKEQWRVLMENAGFESVKLSNYAVSQAKILLWNY 583

Query: 530 D-NNWEMRIEEKDGCVGLWWKGQPVSFCSFWK 547
           + +N    +E K G + L W   P+   S W+
Sbjct: 584 NYSNLYSIVESKPGFISLAWNDLPLLTLSSWR 584

BLAST of Tan0007499.1 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 109.8 bits (273), Expect = 7.3e-24
Identity = 105/415 (25.30%), Postives = 187/415 (45.06%), Query Frame = 0

Query: 142 KNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPTGDANH 201
           ++S +ST +     S + G      L  CA AI + +      L+  +  LA     A  
Sbjct: 159 ESSDESTRSVVLVDSQETGVRLVHALVACAEAIHQENLNLADALVKRVGTLAGSQAGAMG 218

Query: 202 RLAAHGLRALAHHLSSNSSSSSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNN 261
           ++A +  +ALA  +  + ++ +           A+ +P F +   + F+E  P+  F + 
Sbjct: 219 KVATYFAQALARRIYRDYTAETD--------VCAAVNPSFEEVLEMHFYESCPYLKFAHF 278

Query: 262 IANSSILHTLSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVIAPSI 321
            AN +IL    E    +R +H++D+G++ G+QWP L++AL  R GGPP   RLT I P  
Sbjct: 279 TANQAIL----EAVTTARRVHVIDLGLNQGMQWPALMQALALRPGGPPS-FRLTGIGP-- 338

Query: 322 EHDENIETPFSIGPPGDNIFSRLLSFAKSLNINLQINRLDSHSLQSLNSQVIGKFPD-EI 381
              EN ++   +G        +L  FA+++ +  +   L + SL  L  ++    P+ E 
Sbjct: 339 PQTENSDSLQQLG-------WKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESET 398

Query: 382 LIVCAQFRLHQLKHYAPDERFEFLQNLREMEPKAVILSENNMACSCNNCGNFDTGFTRRV 441
           L+V + F LH+L   +     + L  ++ ++P  V + E     + +N   F   F   +
Sbjct: 399 LVVNSVFELHRLLARSGSIE-KLLNTVKAIKPSIVTVVEQE---ANHNGIVFLDRFNEAL 458

Query: 442 EYLWRFLDSTSSAFKGRESDE--------RRVMEGEAAKALTNQGEMNEEKEKWYERMRN 501
            Y     DS   ++     D         R+++   AA+  +++ E +E   +W  RM++
Sbjct: 459 HYYSSLFDSLEDSYSLPSQDRVMSEVYLGRQILNVVAAEG-SDRVERHETAAQWRIRMKS 518

Query: 502 AGFVRKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKL 548
           AGF            A   +  Y      R+EE DGC+ + W+ +P+   S WKL
Sbjct: 519 AGFDPIHLGSSAFKQASMLLSLYATGDGYRVEENDGCLMIGWQTRPLITTSAWKL 546