Tan0003703.4 (mRNA) Snake gourd v1

Overview


Name	Tan0003703.4
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	beta-carotene hydroxylase 2, chloroplastic-like
Location	LG01: 1800328 .. 1803608 (+)
Sequence length	2720
RNA-Seq Expression	Tan0003703.4
Synteny	Tan0003703.4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGTCCTCTCTCTCTATCTCTCTATAGAAACTTGAATTGTTTTTGTTTTCATTGGCGCCGTATTATTCTGACTGAATATTGAATTCCGATTGTTTAATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTACGTTTTCAATTTCATTTCATCTCTGTATTCTCTTCTTTTCTCGAAGAATTCGATTTTCTGTAGGCTGATTTTCACAATCAATTTGGGAATATTCAGGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTATAGGTTTCTAATTCTTCTTCTCAATAGAATTTCGTTTCTGTGTTTGTCTGCTGAGAAAATTTCTATCAACAATAATCCACAATGAATCTTAATTTCATATCAAACAAACAATATTCAAAAGGGAAAAGAGAATCGTTGAGGCTTGGACTTGTTGATTGCAGCACAGTCCTTGCACTATTCCTAAACTAATCCTCCTCTCTGTAGAATTTTTCTTGTTTGGCTGCTGAGAAAATTCAATTAACAATGGACCTTTCTCTCATTTTCCTAGCAAACAAACAACATTGAATAAAAAAAGAAAAATCGTTGACGCTTGGACTTTTTTTTTTTTGGTTGATCTTTATTATTACAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCCATATCTTTCTTCAATTTCTCTCTCATAATCTTTCAGAATCTCAGTTTGTTCATATTCTAACTATTTAATAGTTATTTAATCACTGGGGATTAGCGCTAATGACAGTGTTAAATGACGAAAATACCCCTAGAAAATGTGGTAATTGAGAAAGAGGTTTGACACGCTGGCGGGTTGTGTTGTGGCAGGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGGTAAATTCAATTTCTAAATTTTTATTTCTTTTTAATTTTTTTTAATGCTTAATCTTCATTCTTCCCTTAAAAAATTATCAAATTTGTTATAATTTTTATCTTATTTTGATCTCAAACTTTTGATACTGACTTTGTTTGTTCCCTTGTGTTGACTTTTCATTTAACAAAAACTCAATTATTTTTCCTAACTTTATATTTTATTTGGATCCCTAGACGTACATTTAAGATGATATTTTTTTTAAGTTGAAATCTTCATTCTCATATTTATTGTACAGGTAAATTCCATGTAGATTTCTATTAATGAAATATATGAATCTAAATATATATATATATGAAAAAACTTATTAAATAAAATTTTAAAATGTCAATTGTTTTAAAAAAAAATTAAGAATATTGAGAAATGTAGAGATAAGAAAGAACCTATAAACTTAGGGGGCGGGGGGGAATATGATTTTAAATCAACCGACTAATAAGAAATTAGCTATGGAGTATGAAAATATAATAATATTCTTAGAGTTTGGGCAATTTTTAATAATCTCGCATTAATTTATGTTTTTACGGCTGAAATTAAATACATGTGTTTTTTTTTTTTGGAGACAAAAAACATGTGTTATTAACCTGCACGTAGAAAAGTAATTACACGTATCTAAGTGTAAATCTAATTTTAAAAGATTATGTCCGATTTAAATATTGAAGCTTATGGTTTTTTTTTTCTGGCTGTAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTCAGTTTCGGGTCTATTTATTTATCTTAAATTTTAAATTTATGGTCTTTTTTTTTTTTAATAAATGGTTTCCTAAAAAATGTTGTGGCGTTTTTTTTAATAAATGGTTTTCTCAAAAATGTTGTGGTGCAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

mRNA sequence

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCCATATCTTTCTTCAATTTCTCTCTCATAATCTTTCAGAATCTCAGTTTGTTCATATTCTAACTATTTAATAGTTATTTAATCACTGGGGATTAGCGCTAATGACAGTGTTAAATGACGAAAATACCCCTAGAAAATGTGGTAATTGAGAAAGAGGTTTGACACGCTGGCGGGTTGTGTTGTGGCAGGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGGTAAATTCAATTTCTAAATTTTTATTTCTTTTTAATTTTTTTTAATGCTTAATCTTCATTCTTCCCTTAAAAAATTATCAAATTTGTTATAATTTTTATCTTATTTTGATCTCAAACTTTTGATACTGACTTTGTTTGTTCCCTTGTGTTGACTTTTCATTTAACAAAAACTCAATTATTTTTCCTAACTTTATATTTTATTTGGATCCCTAGACGTACATTTAAGATGATATTTTTTTTAAGTTGAAATCTTCATTCTCATATTTATTGTACAGGTAAATTCCATGTAGATTTCTATTAATGAAATATATGAATCTAAATATATATATATATGAAAAAACTTATTAAATAAAATTTTAAAATGTCAATTGTTTTAAAAAAAAATTAAGAATATTGAGAAATGTAGAGATAAGAAAGAACCTATAAACTTAGGGGGCGGGGGGGAATATGATTTTAAATCAACCGACTAATAAGAAATTAGCTATGGAGTATGAAAATATAATAATATTCTTAGAGTTTGGGCAATTTTTAATAATCTCGCATTAATTTATGTTTTTACGGCTGAAATTAAATACATGTGTTTTTTTTTTTTGGAGACAAAAAACATGTGTTATTAACCTGCACGTAGAAAAGTAATTACACGTATCTAAGTGTAAATCTAATTTTAAAAGATTATGTCCGATTTAAATATTGAAGCTTATGGTTTTTTTTTTCTGGCTGTAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTCAGTTTCGGGTCTATTTATTTATCTTAAATTTTAAATTTATGGTCTTTTTTTTTTTTAATAAATGGTTTCCTAAAAAATGTTGTGGCGTTTTTTTTAATAAATGGTTTTCTCAAAAATGTTGTGGTGCAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCCATATCTTTCTTCAATTTCTCTCTCATAATCTTTCAGAATCTCAGTTTGTTCATATTCTAACTATTTAA

Protein sequence

MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAVNLHIFLQFLSHNLSESQFVHILTI

Homology

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BLAST of Tan0003703.4 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 285.4 bits (729), Expect = 6.0e-76
Identity = 142/196 (72.45%), Postives = 158/196 (80.61%), Query Frame = 0


Query: 25  PKSRTPFLFPRSVFR-NSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQI 84
           P   TP ++P + F  N     + RRR   TVC  +ED     + E   ++    I  QI
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 86

Query: 85  PSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  +AE+LARK+SERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 87  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 146

Query: 145 SLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLS 204
           +LSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 
Sbjct: 147 ALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLD 206

Query: 205 YGFFHKGLVPGLCFGA 220
           YGFFHKGL+PGLCFGA
Sbjct: 207 YGFFHKGLIPGLCFGA 222

[Close]

BLAST of Tan0003703.4 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-71
Identity = 141/215 (65.58%), Postives = 166/215 (77.21%), Query Frame = 0


Query: 18  LTSSNLSPKS-----RTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGE--VEN 77
           ++SS+L+PKS      TP L     F+      + R +  LTVC  +E++   G+  V +
Sbjct: 21  ISSSSLTPKSSPVSTSTPTLVVFPPFKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVAS 80

Query: 78  LADEGSPIIVPQ------IPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYR 137
             D+G+  +  Q        +  +A++LARK+SERFTYLVAAVMSSFGITSMAV++VYYR
Sbjct: 81  DDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYR 140

Query: 138 FYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPF 197
           F WQMEGGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWHASLWHMHESHHKPREGPF
Sbjct: 141 FSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPF 200

Query: 198 ELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           ELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGA
Sbjct: 201 ELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGA 235

[Close]

BLAST of Tan0003703.4 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 3.9e-67
Identity = 138/223 (61.88%), Postives = 163/223 (73.09%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of Tan0003703.4 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 247.3 bits (630), Expect = 1.8e-64
Identity = 126/198 (63.64%), Postives = 149/198 (75.25%), Query Frame = 0


Query: 30  PFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQN----SSGEVENLADEGSPIIV----P 89
           P LF   +  N     + RR+  L  C  ++D       SG+  +    G  I V     
Sbjct: 32  PLLFFSPLTCNLDAILRSRRKPRLAACFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEE 91

Query: 90  QIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFG 149
           +  +  +AE+ ARK+SERFTYLVAAVMSS GITSMAV++VYYRF WQMEGGE+PFSEMF 
Sbjct: 92  KSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFC 151

Query: 150 TFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIAL 209
           TF+L+ GAA+GME+WARWAHRALWHASLWHMHESHH+PREGPFELND+FAIINAVPAIAL
Sbjct: 152 TFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIAL 211

Query: 210 LSYGFFHKGLVPGLCFGA 220
           LS+GF HKGL+PGLCFGA
Sbjct: 212 LSFGFNHKGLIPGLCFGA 229

[Close]

BLAST of Tan0003703.4 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 6.9e-64
Identity = 136/224 (60.71%), Postives = 161/224 (71.88%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGA
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGA 213

[Close]

BLAST of Tan0003703.4 vs. NCBI nr
Match: XP_022944865.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 419.1 bits (1076), Expect = 2.6e-113
Identity = 204/219 (93.15%), Postives = 211/219 (96.35%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 419.1 bits (1076), Expect = 2.6e-113
Identity = 204/219 (93.15%), Postives = 211/219 (96.35%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. NCBI nr
Match: XP_022968453.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 417.9 bits (1073), Expect = 5.9e-113
Identity = 212/243 (87.24%), Postives = 220/243 (90.53%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAVNLHIFLQFLSHNLSESQFVH 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAVNL + L     NLS     H
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAVNLSLSLAIFP-NLS---LFH 239

Query: 241 ILT 244
           ILT
Sbjct: 241 ILT 239

[Close]

BLAST of Tan0003703.4 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 417.5 bits (1072), Expect = 7.7e-113
Identity = 205/219 (93.61%), Postives = 210/219 (95.89%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAAL PKP HL LTSS+LSPK R PFLFP SVFRNSRIQWKMRRRTL TVCV VE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVENL+DE SPIIVPQIPSPHV+ERLARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. NCBI nr
Match: KAG6574199.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 416.0 bits (1068), Expect = 2.2e-112
Identity = 203/219 (92.69%), Postives = 210/219 (95.89%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPK FH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKSFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.3e-113
Identity = 204/219 (93.15%), Postives = 211/219 (96.35%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. ExPASy TrEMBL
Match: A0A6J1HY20 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 2.9e-113
Identity = 212/243 (87.24%), Postives = 220/243 (90.53%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAVNLHIFLQFLSHNLSESQFVH 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAVNL + L     NLS     H
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAVNLSLSLAIFP-NLS---LFH 239

Query: 241 ILT 244
           ILT
Sbjct: 241 ILT 239

[Close]

BLAST of Tan0003703.4 vs. ExPASy TrEMBL
Match: A0A6J1HX82 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 4.6e-111
Identity = 201/219 (91.78%), Postives = 208/219 (94.98%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. ExPASy TrEMBL
Match: G1FQR0 (Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 4.6e-111
Identity = 201/219 (91.78%), Postives = 208/219 (94.98%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.8e-111
Identity = 198/219 (90.41%), Postives = 210/219 (95.89%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAA  PKPFHLFLTSS+LSPK  +PFLFP SVFRNSR QWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
            QNSSG+V+NL+DE SPI+VPQIPSPHV+ERLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGA
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGA 219

[Close]

BLAST of Tan0003703.4 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 258.5 bits (659), Expect = 5.6e-69
Identity = 139/225 (61.78%), Postives = 165/225 (73.33%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAVN 222
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAV+
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAVS 217

[Close]

BLAST of Tan0003703.4 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 256.1 bits (653), Expect = 2.8e-68
Identity = 138/223 (61.88%), Postives = 163/223 (73.09%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of Tan0003703.4 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 245.4 bits (625), Expect = 4.9e-65
Identity = 136/224 (60.71%), Postives = 161/224 (71.88%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGA
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGA 213

[Close]

BLAST of Tan0003703.4 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-25
Identity = 75/155 (48.39%), Postives = 98/155 (63.23%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAA 151
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAA
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAA 144

The following BLAST results are available for this feature:

BLAST of Tan0003703.4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	6.0e-76	72.45	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	1.5e-71	65.58	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	3.9e-67	61.88	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	1.8e-64	63.64	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9LTG0	6.9e-64	60.71	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Tan0003703.4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944865.1	2.6e-113	93.15	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
KAG7013258.1	2.6e-113	93.15	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_022968453.1	5.9e-113	87.24	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]
XP_038877448.1	7.7e-113	93.61	beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida]	[more]
KAG6574199.1	2.2e-112	92.69	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]

BLAST of Tan0003703.4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ94	1.3e-113	93.15	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata...	[more]
A0A6J1HY20	2.9e-113	87.24	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita maxima O...	[more]
A0A6J1HX82	4.6e-111	91.78	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima O...	[more]
G1FQR0	4.6e-111	91.78	Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2...	[more]
A0A0A0L606	7.8e-111	90.41	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]

BLAST of Tan0003703.4 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.2	5.6e-69	61.78	beta-hydroxylase 1	[more]
AT4G25700.1	2.8e-68	61.88	beta-hydroxylase 1	[more]
AT5G52570.1	4.9e-65	60.71	beta-carotene hydroxylase 2	[more]
AT5G52570.2	1.3e-25	48.39	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 1..220

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0003703	Tan0003703	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.4-five_prime_utr	Tan0003703.4-five_prime_utr-LG01:1800328..1800683	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.4-exon	Tan0003703.4-exon-LG01:1800328..1801073	exon
Tan0003703.4-exon	Tan0003703.4-exon-LG01:1801184..1801243	exon
Tan0003703.4-exon	Tan0003703.4-exon-LG01:1801343..1801414	exon
Tan0003703.4-exon	Tan0003703.4-exon-LG01:1801767..1803608	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.4-cds	Tan0003703.4-cds-LG01:1800684..1801073	CDS
Tan0003703.4-cds	Tan0003703.4-cds-LG01:1801184..1801243	CDS
Tan0003703.4-cds	Tan0003703.4-cds-LG01:1801343..1801414	CDS
Tan0003703.4-cds	Tan0003703.4-cds-LG01:1801767..1801979	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.4-three_prime_utr	Tan0003703.4-three_prime_utr-LG01:1801980..1803608	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0003703.4	Tan0003703.4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0005506	iron ion binding