Tan0003703.3 (mRNA) Snake gourd v1

Overview


Name	Tan0003703.3
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	beta-carotene hydroxylase 2, chloroplastic-like
Location	LG01: 1800328 .. 1803608 (+)
Sequence length	1802
RNA-Seq Expression	Tan0003703.3
Synteny	Tan0003703.3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGTCCTCTCTCTCTATCTCTCTATAGAAACTTGAATTGTTTTTGTTTTCATTGGCGCCGTATTATTCTGACTGAATATTGAATTCCGATTGTTTAATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTACGTTTTCAATTTCATTTCATCTCTGTATTCTCTTCTTTTCTCGAAGAATTCGATTTTCTGTAGGCTGATTTTCACAATCAATTTGGGAATATTCAGGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTATAGGTTTCTAATTCTTCTTCTCAATAGAATTTCGTTTCTGTGTTTGTCTGCTGAGAAAATTTCTATCAACAATAATCCACAATGAATCTTAATTTCATATCAAACAAACAATATTCAAAAGGGAAAAGAGAATCGTTGAGGCTTGGACTTGTTGATTGCAGCACAGTCCTTGCACTATTCCTAAACTAATCCTCCTCTCTGTAGAATTTTTCTTGTTTGGCTGCTGAGAAAATTCAATTAACAATGGACCTTTCTCTCATTTTCCTAGCAAACAAACAACATTGAATAAAAAAAGAAAAATCGTTGACGCTTGGACTTTTTTTTTTTTGGTTGATCTTTATTATTACAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCCATATCTTTCTTCAATTTCTCTCTCATAATCTTTCAGAATCTCAGTTTGTTCATATTCTAACTATTTAATAGTTATTTAATCACTGGGGATTAGCGCTAATGACAGTGTTAAATGACGAAAATACCCCTAGAAAATGTGGTAATTGAGAAAGAGGTTTGACACGCTGGCGGGTTGTGTTGTGGCAGGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGGTAAATTCAATTTCTAAATTTTTATTTCTTTTTAATTTTTTTTAATGCTTAATCTTCATTCTTCCCTTAAAAAATTATCAAATTTGTTATAATTTTTATCTTATTTTGATCTCAAACTTTTGATACTGACTTTGTTTGTTCCCTTGTGTTGACTTTTCATTTAACAAAAACTCAATTATTTTTCCTAACTTTATATTTTATTTGGATCCCTAGACGTACATTTAAGATGATATTTTTTTTAAGTTGAAATCTTCATTCTCATATTTATTGTACAGGTAAATTCCATGTAGATTTCTATTAATGAAATATATGAATCTAAATATATATATATATGAAAAAACTTATTAAATAAAATTTTAAAATGTCAATTGTTTTAAAAAAAAATTAAGAATATTGAGAAATGTAGAGATAAGAAAGAACCTATAAACTTAGGGGGCGGGGGGGAATATGATTTTAAATCAACCGACTAATAAGAAATTAGCTATGGAGTATGAAAATATAATAATATTCTTAGAGTTTGGGCAATTTTTAATAATCTCGCATTAATTTATGTTTTTACGGCTGAAATTAAATACATGTGTTTTTTTTTTTTGGAGACAAAAAACATGTGTTATTAACCTGCACGTAGAAAAGTAATTACACGTATCTAAGTGTAAATCTAATTTTAAAAGATTATGTCCGATTTAAATATTGAAGCTTATGGTTTTTTTTTTCTGGCTGTAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTCAGTTTCGGGTCTATTTATTTATCTTAAATTTTAAATTTATGGTCTTTTTTTTTTTTAATAAATGGTTTCCTAAAAAATGTTGTGGCGTTTTTTTTAATAAATGGTTTTCTCAAAAATGTTGTGGTGCAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

mRNA sequence

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTCAGTTTCGGGTCTATTTATTTATCTTAAATTTTAAATTTATGGTCTTTTTTTTTTTTAATAAATGGTTTCCTAAAAAATGTTGTGGCGTTTTTTTTAATAAATGGTTTTCTCAAAAATGTTGTGGTGCAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTCAGTTTCGGGTCTATTTATTTATCTTAA

Protein sequence

MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKVSFGSIYLS

Homology

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BLAST of Tan0003703.3 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 404.4 bits (1038), Expect = 1.0e-111
Identity = 198/257 (77.04%), Postives = 216/257 (84.05%), Query Frame = 0


Query: 25  PKSRTPFLFPRSVFR-NSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQI 84
           P   TP ++P + F  N     + RRR   TVC  +ED     + E   ++    I  QI
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 86

Query: 85  PSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  +AE+LARK+SERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 87  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 146

Query: 145 SLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLS 204
           +LSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 
Sbjct: 147 ALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLD 206

Query: 205 YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQLH 264
           YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF VGP+ANVPY RKVAAAH LH
Sbjct: 207 YGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSLH 266

Query: 265 HSDKFNGVPYGLFLGPK 281
           HS+KFNGVPYGLFLGPK
Sbjct: 267 HSEKFNGVPYGLFLGPK 283

[Close]

BLAST of Tan0003703.3 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.6e-107
Identity = 198/276 (71.74%), Postives = 225/276 (81.52%), Query Frame = 0


Query: 18  LTSSNLSPKS-----RTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGE--VEN 77
           ++SS+L+PKS      TP L     F+      + R +  LTVC  +E++   G+  V +
Sbjct: 21  ISSSSLTPKSSPVSTSTPTLVVFPPFKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVAS 80

Query: 78  LADEGSPIIVPQ------IPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYR 137
             D+G+  +  Q        +  +A++LARK+SERFTYLVAAVMSSFGITSMAV++VYYR
Sbjct: 81  DDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYR 140

Query: 138 FYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPF 197
           F WQMEGGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWHASLWHMHESHHKPREGPF
Sbjct: 141 FSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPF 200

Query: 198 ELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLV 257
           ELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF V
Sbjct: 201 ELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPV 260

Query: 258 GPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           GPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPK
Sbjct: 261 GPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPK 296

[Close]

BLAST of Tan0003703.3 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-102
Identity = 194/284 (68.31%), Postives = 222/284 (78.17%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDG
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDG 240

Query: 241 LVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           LVHKRF VGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPK
Sbjct: 241 LVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPK 276

[Close]

BLAST of Tan0003703.3 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 1.1e-100
Identity = 183/259 (70.66%), Postives = 209/259 (80.69%), Query Frame = 0


Query: 30  PFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQN----SSGEVENLADEGSPIIV----P 89
           P LF   +  N     + RR+  L  C  ++D       SG+  +    G  I V     
Sbjct: 32  PLLFFSPLTCNLDAILRSRRKPRLAACFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEE 91

Query: 90  QIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFG 149
           +  +  +AE+ ARK+SERFTYLVAAVMSS GITSMAV++VYYRF WQMEGGE+PFSEMF 
Sbjct: 92  KSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFC 151

Query: 150 TFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIAL 209
           TF+L+ GAA+GME+WARWAHRALWHASLWHMHESHH+PREGPFELND+FAIINAVPAIAL
Sbjct: 152 TFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIAL 211

Query: 210 LSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQ 269
           LS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF VGPIANVPYF++VAAAHQ
Sbjct: 212 LSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQ 271

Query: 270 LHHSDKFNGVPYGLFLGPK 281
           LHHSDKF+GVPYGLFLGPK
Sbjct: 272 LHHSDKFDGVPYGLFLGPK 290

[Close]

BLAST of Tan0003703.3 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.0e-99
Identity = 192/285 (67.37%), Postives = 219/285 (76.84%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPK
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPK 274

[Close]

BLAST of Tan0003703.3 vs. NCBI nr
Match: XP_022944865.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 263/280 (93.93%), Postives = 271/280 (96.79%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 265/280 (94.64%), Postives = 270/280 (96.43%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAAL PKP HL LTSS+LSPK R PFLFP SVFRNSRIQWKMRRRTL TVCV VE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVENL+DE SPIIVPQIPSPHV+ERLARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 263/280 (93.93%), Postives = 271/280 (96.79%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. NCBI nr
Match: KAG6574199.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 540.0 bits (1390), Expect = 1.2e-149
Identity = 262/280 (93.57%), Postives = 270/280 (96.43%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPK FH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKSFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. NCBI nr
Match: XP_023541483.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.3 bits (1383), Expect = 7.9e-149
Identity = 259/280 (92.50%), Postives = 270/280 (96.43%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA +SA LGPKPFH+FLTSSNLSPK RTPFL+P SVFR+SRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASVSAVLGPKPFHVFLTSSNLSPKPRTPFLYPSSVFRSSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADENLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.0e-151
Identity = 263/280 (93.93%), Postives = 271/280 (96.79%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.5e-148
Identity = 258/280 (92.14%), Postives = 270/280 (96.43%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAA  PKPFHLFLTSS+LSPK  +PFLFP SVFRNSR QWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
            QNSSG+V+NL+DE SPI+VPQIPSPHV+ERLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. ExPASy TrEMBL
Match: A0A6J1HX82 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 259/280 (92.50%), Postives = 267/280 (95.36%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. ExPASy TrEMBL
Match: G1FQR0 (Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 259/280 (92.50%), Postives = 267/280 (95.36%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.7e-147
Identity = 259/280 (92.50%), Postives = 267/280 (95.36%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSAALGPKP HLFL SS+LS K RTPFLFP SVFRNSRIQ KMRR+TL TVCV VE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVENL+DE  PI+VPQIPSP+VA+RLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWH+S+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 280

[Close]

BLAST of Tan0003703.3 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 374.4 bits (960), Expect = 8.2e-104
Identity = 194/284 (68.31%), Postives = 222/284 (78.17%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDG
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDG 240

Query: 241 LVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           LVHKRF VGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPK
Sbjct: 241 LVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPK 276

[Close]

BLAST of Tan0003703.3 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 363.6 bits (932), Expect = 1.5e-100
Identity = 192/285 (67.37%), Postives = 219/285 (76.84%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPK
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPK 274

[Close]

BLAST of Tan0003703.3 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 255.8 bits (652), Expect = 4.3e-68
Identity = 138/223 (61.88%), Postives = 163/223 (73.09%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of Tan0003703.3 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 202.6 bits (514), Expect = 4.3e-52
Identity = 131/285 (45.96%), Postives = 156/285 (54.74%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGA                          
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGA-------------------------- 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
                                                      AGLGIT+FGMAYMFVHD
Sbjct: 181 -------------------------------------------AGLGITMFGMAYMFVHD 205

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 281
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPK
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPK 205

The following BLAST results are available for this feature:

BLAST of Tan0003703.3 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	1.0e-111	77.04	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	1.6e-107	71.74	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	1.2e-102	68.31	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	1.1e-100	70.66	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9LTG0	2.0e-99	67.37	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Tan0003703.3 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944865.1	1.4e-150	93.93	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
XP_038877448.1	1.4e-150	94.64	beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida]	[more]
KAG7013258.1	1.4e-150	93.93	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
KAG6574199.1	1.2e-149	93.57	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_023541483.1	7.9e-149	92.50	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp...	[more]

BLAST of Tan0003703.3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ94	7.0e-151	93.93	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata...	[more]
A0A0A0L606	1.5e-148	92.14	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1HX82	1.2e-147	92.50	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima O...	[more]
G1FQR0	1.2e-147	92.50	Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2...	[more]
A0A6J1J1S8	2.7e-147	92.50	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Tan0003703.3 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	8.2e-104	68.31	beta-hydroxylase 1	[more]
AT5G52570.1	1.5e-100	67.37	beta-carotene hydroxylase 2	[more]
AT4G25700.2	4.3e-68	61.88	beta-hydroxylase 1	[more]
AT5G52570.2	4.3e-52	45.96	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 149..276 e-value: 2.5E-13 score: 50.5
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 17..280
None	No IPR available	PANTHER	PTHR31899:SF10	BETA-CAROTENE HYDROXYLASE	coord: 17..280

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0003703	Tan0003703	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.3-five_prime_utr	Tan0003703.3-five_prime_utr-LG01:1800328..1800683	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.3-exon	Tan0003703.3-exon-LG01:1800328..1801073	exon
Tan0003703.3-exon	Tan0003703.3-exon-LG01:1801184..1801243	exon
Tan0003703.3-exon	Tan0003703.3-exon-LG01:1801343..1801414	exon
Tan0003703.3-exon	Tan0003703.3-exon-LG01:1801767..1801901	exon
Tan0003703.3-exon	Tan0003703.3-exon-LG01:1802097..1802222	exon
Tan0003703.3-exon	Tan0003703.3-exon-LG01:1802946..1803608	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.3-cds	Tan0003703.3-cds-LG01:1800684..1801073	CDS
Tan0003703.3-cds	Tan0003703.3-cds-LG01:1801184..1801243	CDS
Tan0003703.3-cds	Tan0003703.3-cds-LG01:1801343..1801414	CDS
Tan0003703.3-cds	Tan0003703.3-cds-LG01:1801767..1801901	CDS
Tan0003703.3-cds	Tan0003703.3-cds-LG01:1802097..1802222	CDS
Tan0003703.3-cds	Tan0003703.3-cds-LG01:1802946..1803032	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.3-three_prime_utr	Tan0003703.3-three_prime_utr-LG01:1803033..1803608	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0003703.3	Tan0003703.3-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity