Tan0003703.1 (mRNA) Snake gourd v1

Overview


Name	Tan0003703.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	beta-carotene hydroxylase 2, chloroplastic-like
Location	LG01: 1800328 .. 1803608 (+)
Sequence length	1670
RNA-Seq Expression	Tan0003703.1
Synteny	Tan0003703.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGTCCTCTCTCTCTATCTCTCTATAGAAACTTGAATTGTTTTTGTTTTCATTGGCGCCGTATTATTCTGACTGAATATTGAATTCCGATTGTTTAATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTACGTTTTCAATTTCATTTCATCTCTGTATTCTCTTCTTTTCTCGAAGAATTCGATTTTCTGTAGGCTGATTTTCACAATCAATTTGGGAATATTCAGGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTATAGGTTTCTAATTCTTCTTCTCAATAGAATTTCGTTTCTGTGTTTGTCTGCTGAGAAAATTTCTATCAACAATAATCCACAATGAATCTTAATTTCATATCAAACAAACAATATTCAAAAGGGAAAAGAGAATCGTTGAGGCTTGGACTTGTTGATTGCAGCACAGTCCTTGCACTATTCCTAAACTAATCCTCCTCTCTGTAGAATTTTTCTTGTTTGGCTGCTGAGAAAATTCAATTAACAATGGACCTTTCTCTCATTTTCCTAGCAAACAAACAACATTGAATAAAAAAAGAAAAATCGTTGACGCTTGGACTTTTTTTTTTTTGGTTGATCTTTATTATTACAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCCATATCTTTCTTCAATTTCTCTCTCATAATCTTTCAGAATCTCAGTTTGTTCATATTCTAACTATTTAATAGTTATTTAATCACTGGGGATTAGCGCTAATGACAGTGTTAAATGACGAAAATACCCCTAGAAAATGTGGTAATTGAGAAAGAGGTTTGACACGCTGGCGGGTTGTGTTGTGGCAGGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGGTAAATTCAATTTCTAAATTTTTATTTCTTTTTAATTTTTTTTAATGCTTAATCTTCATTCTTCCCTTAAAAAATTATCAAATTTGTTATAATTTTTATCTTATTTTGATCTCAAACTTTTGATACTGACTTTGTTTGTTCCCTTGTGTTGACTTTTCATTTAACAAAAACTCAATTATTTTTCCTAACTTTATATTTTATTTGGATCCCTAGACGTACATTTAAGATGATATTTTTTTTAAGTTGAAATCTTCATTCTCATATTTATTGTACAGGTAAATTCCATGTAGATTTCTATTAATGAAATATATGAATCTAAATATATATATATATGAAAAAACTTATTAAATAAAATTTTAAAATGTCAATTGTTTTAAAAAAAAATTAAGAATATTGAGAAATGTAGAGATAAGAAAGAACCTATAAACTTAGGGGGCGGGGGGGAATATGATTTTAAATCAACCGACTAATAAGAAATTAGCTATGGAGTATGAAAATATAATAATATTCTTAGAGTTTGGGCAATTTTTAATAATCTCGCATTAATTTATGTTTTTACGGCTGAAATTAAATACATGTGTTTTTTTTTTTTGGAGACAAAAAACATGTGTTATTAACCTGCACGTAGAAAAGTAATTACACGTATCTAAGTGTAAATCTAATTTTAAAAGATTATGTCCGATTTAAATATTGAAGCTTATGGTTTTTTTTTTCTGGCTGTAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTCAGTTTCGGGTCTATTTATTTATCTTAAATTTTAAATTTATGGTCTTTTTTTTTTTTAATAAATGGTTTCCTAAAAAATGTTGTGGCGTTTTTTTTAATAAATGGTTTTCTCAAAAATGTTGTGGTGCAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

mRNA sequence

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGA

Protein sequence

MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKSTATKSIDGS

Homology

[Close]

BLAST of Tan0003703.1 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 435.3 bits (1118), Expect = 5.9e-121
Identity = 218/287 (75.96%), Postives = 238/287 (82.93%), Query Frame = 0


Query: 25  PKSRTPFLFPRSVFR-NSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQI 84
           P   TP ++P + F  N     + RRR   TVC  +ED     + E   ++    I  QI
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 86

Query: 85  PSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  +AE+LARK+SERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 87  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 146

Query: 145 SLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLS 204
           +LSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 
Sbjct: 147 ALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLD 206

Query: 205 YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQLH 264
           YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF VGP+ANVPY RKVAAAH LH
Sbjct: 207 YGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSLH 266

Query: 265 HSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKSTATKSIDGS 311
           HS+KFNGVPYGLFLGPKELEEVGG EELEKE+NRR     T+ I GS
Sbjct: 267 HSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRR-----TRYIKGS 308

[Close]

BLAST of Tan0003703.1 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.7e-115
Identity = 213/297 (71.72%), Postives = 242/297 (81.48%), Query Frame = 0


Query: 18  LTSSNLSPKS-----RTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGE--VEN 77
           ++SS+L+PKS      TP L     F+      + R +  LTVC  +E++   G+  V +
Sbjct: 21  ISSSSLTPKSSPVSTSTPTLVVFPPFKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVAS 80

Query: 78  LADEGSPIIVPQ------IPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYR 137
             D+G+  +  Q        +  +A++LARK+SERFTYLVAAVMSSFGITSMAV++VYYR
Sbjct: 81  DDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYR 140

Query: 138 FYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPF 197
           F WQMEGGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWHASLWHMHESHHKPREGPF
Sbjct: 141 FSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPF 200

Query: 198 ELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLV 257
           ELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF V
Sbjct: 201 ELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPV 260

Query: 258 GPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKS 302
           GPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPKELEEVGG + LE EINRR K+
Sbjct: 261 GPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRTKN 317

[Close]

BLAST of Tan0003703.1 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.5e-113
Identity = 215/314 (68.47%), Postives = 245/314 (78.03%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDG
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDG 240

Query: 241 LVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEIN 300
           LVHKRF VGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+
Sbjct: 241 LVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKEIS 300

Query: 301 RRIKSTATKSIDGS 311
           RRIKS    S  GS
Sbjct: 301 RRIKSYKKASGSGS 306

[Close]

BLAST of Tan0003703.1 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 399.8 bits (1026), Expect = 2.8e-110
Identity = 201/281 (71.53%), Postives = 229/281 (81.49%), Query Frame = 0


Query: 30  PFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQN----SSGEVENLADEGSPIIV----P 89
           P LF   +  N     + RR+  L  C  ++D       SG+  +    G  I V     
Sbjct: 32  PLLFFSPLTCNLDAILRSRRKPRLAACFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEE 91

Query: 90  QIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFG 149
           +  +  +AE+ ARK+SERFTYLVAAVMSS GITSMAV++VYYRF WQMEGGE+PFSEMF 
Sbjct: 92  KSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFC 151

Query: 150 TFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIAL 209
           TF+L+ GAA+GME+WARWAHRALWHASLWHMHESHH+PREGPFELND+FAIINAVPAIAL
Sbjct: 152 TFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIAL 211

Query: 210 LSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQ 269
           LS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF VGPIANVPYF++VAAAHQ
Sbjct: 212 LSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQ 271

Query: 270 LHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKST 303
           LHHSDKF+GVPYGLFLGPKELEEVG  EELEKE+NRRIKS+
Sbjct: 272 LHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIKSS 312

[Close]

BLAST of Tan0003703.1 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 4.0e-109
Identity = 209/305 (68.52%), Postives = 239/305 (78.36%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEI 300
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 294

[Close]

BLAST of Tan0003703.1 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 588.2 bits (1515), Expect = 4.2e-164
Identity = 291/310 (93.87%), Postives = 296/310 (95.48%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAAL PKP HL LTSS+LSPK R PFLFP SVFRNSRIQWKMRRRTL TVCV VE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVENL+DE SPIIVPQIPSPHV+ERLARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
             A KSIDGS
Sbjct: 301 WAAPKSIDGS 310

[Close]

BLAST of Tan0003703.1 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.0 bits (1512), Expect = 9.3e-164
Identity = 288/310 (92.90%), Postives = 298/310 (96.13%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG EELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703.1 vs. NCBI nr
Match: XP_022944865.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 585.9 bits (1509), Expect = 2.1e-163
Identity = 287/310 (92.58%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG +ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703.1 vs. NCBI nr
Match: KAG6574199.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583.9 bits (1504), Expect = 7.9e-163
Identity = 287/310 (92.58%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPK FH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKSFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG EELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703.1 vs. NCBI nr
Match: XP_023541483.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.2 bits (1502), Expect = 1.3e-162
Identity = 285/310 (91.94%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA +SA LGPKPFH+FLTSSNLSPK RTPFL+P SVFR+SRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASVSAVLGPKPFHVFLTSSNLSPKPRTPFLYPSSVFRSSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADENLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703.1 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.0e-163
Identity = 287/310 (92.58%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG +ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703.1 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 285/310 (91.94%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAA  PKPFHLFLTSS+LSPK  +PFLFP SVFRNSR QWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
            QNSSG+V+NL+DE SPI+VPQIPSPHV+ERLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
            TA KSIDGS
Sbjct: 301 WTARKSIDGS 310

[Close]

BLAST of Tan0003703.1 vs. ExPASy TrEMBL
Match: A0A6J1E4L5 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430578 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.5e-162
Identity = 285/310 (91.94%), Postives = 296/310 (95.48%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA +S ALGP+P HLFL SS+LSPK RTPFLFP SVFRNSRIQ K+RR+TL TVCV VE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVE+L+DE  PI+VPQIPSP+VA+RLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
            T TKSIDGS
Sbjct: 301 LTTTKSIDGS 310

[Close]

BLAST of Tan0003703.1 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.5e-162
Identity = 286/310 (92.26%), Postives = 295/310 (95.16%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSAALGPKP HLFL SS+LS K RTPFLFP SVFRNSRIQ KMRR+TL TVCV VE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVENL+DE  PI+VPQIPSP+VA+RLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWH+S+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
            T TKSI+GS
Sbjct: 301 LTTTKSINGS 310

[Close]

BLAST of Tan0003703.1 vs. ExPASy TrEMBL
Match: A0A6J1HX82 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.1e-161
Identity = 285/310 (91.94%), Postives = 294/310 (94.84%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703.1 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.5e-114
Identity = 215/314 (68.47%), Postives = 245/314 (78.03%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDG
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDG 240

Query: 241 LVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEIN 300
           LVHKRF VGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+
Sbjct: 241 LVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKEIS 300

Query: 301 RRIKSTATKSIDGS 311
           RRIKS    S  GS
Sbjct: 301 RRIKSYKKASGSGS 306

[Close]

BLAST of Tan0003703.1 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 396.0 bits (1016), Expect = 2.8e-110
Identity = 209/305 (68.52%), Postives = 239/305 (78.36%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEI 300
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 294

[Close]

BLAST of Tan0003703.1 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 255.8 bits (652), Expect = 4.6e-68
Identity = 138/223 (61.88%), Postives = 163/223 (73.09%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of Tan0003703.1 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-61
Identity = 148/305 (48.52%), Postives = 176/305 (57.70%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGA                          
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGA-------------------------- 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
                                                      AGLGIT+FGMAYMFVHD
Sbjct: 181 -------------------------------------------AGLGITMFGMAYMFVHD 225

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEI 300
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 225

The following BLAST results are available for this feature:

BLAST of Tan0003703.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	5.9e-121	75.96	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	1.7e-115	71.72	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	3.5e-113	68.47	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	2.8e-110	71.53	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9LTG0	4.0e-109	68.52	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Tan0003703.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877448.1	4.2e-164	93.87	beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida]	[more]
KAG7013258.1	9.3e-164	92.90	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_022944865.1	2.1e-163	92.58	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
KAG6574199.1	7.9e-163	92.58	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_023541483.1	1.3e-162	91.94	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp...	[more]

BLAST of Tan0003703.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ94	1.0e-163	92.58	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata...	[more]
A0A0A0L606	1.1e-162	91.94	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1E4L5	2.5e-162	91.94	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1J1S8	5.5e-162	92.26	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1HX82	2.1e-161	91.94	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima O...	[more]

BLAST of Tan0003703.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	2.5e-114	68.47	beta-hydroxylase 1	[more]
AT5G52570.1	2.8e-110	68.52	beta-carotene hydroxylase 2	[more]
AT4G25700.2	4.6e-68	61.88	beta-hydroxylase 1	[more]
AT5G52570.2	1.1e-61	48.52	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 149..276 e-value: 3.6E-13 score: 50.0
None	No IPR available	PANTHER	PTHR31899:SF10	BETA-CAROTENE HYDROXYLASE	coord: 17..301
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 17..301

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0003703	Tan0003703	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.1-five_prime_utr	Tan0003703.1-five_prime_utr-LG01:1800328..1800683	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.1-exon	Tan0003703.1-exon-LG01:1800328..1801073	exon
Tan0003703.1-exon	Tan0003703.1-exon-LG01:1801184..1801243	exon
Tan0003703.1-exon	Tan0003703.1-exon-LG01:1801343..1801414	exon
Tan0003703.1-exon	Tan0003703.1-exon-LG01:1801767..1801901	exon
Tan0003703.1-exon	Tan0003703.1-exon-LG01:1802097..1802222	exon
Tan0003703.1-exon	Tan0003703.1-exon-LG01:1802946..1803002	exon
Tan0003703.1-exon	Tan0003703.1-exon-LG01:1803135..1803608	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.1-cds	Tan0003703.1-cds-LG01:1800684..1801073	CDS
Tan0003703.1-cds	Tan0003703.1-cds-LG01:1801184..1801243	CDS
Tan0003703.1-cds	Tan0003703.1-cds-LG01:1801343..1801414	CDS
Tan0003703.1-cds	Tan0003703.1-cds-LG01:1801767..1801901	CDS
Tan0003703.1-cds	Tan0003703.1-cds-LG01:1802097..1802222	CDS
Tan0003703.1-cds	Tan0003703.1-cds-LG01:1802946..1803002	CDS
Tan0003703.1-cds	Tan0003703.1-cds-LG01:1803135..1803227	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003703.1-three_prime_utr	Tan0003703.1-three_prime_utr-LG01:1803228..1803608	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0003703.1	Tan0003703.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity