Tan0003473.1 (mRNA) Snake gourd v1

Overview


Name	Tan0003473.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF-hand family protein
Location	LG11: 10451574 .. 10452323 (-)
Sequence length	750
RNA-Seq Expression	Tan0003473.1
Synteny	Tan0003473.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATATTTCTAAGCCAAAGAACCAAAAATTACCATGTCTCCTATTAGCTCCAACGACTTGCTCAGGATCTTCAACAAGCTCGACAAGAACCACGATGGCCTCATTTGCCTACACGAGCTCAACTGGCTCCTCCACACCATTGGCATCCAACTAACCATGGAGGAGTTGGAGTCTTTCCTCGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTACGAATCGATATCGAAGCAAAACAATGCCGAGTGCAAGGGAGGCGTAGGCGATGACGACGATGCAGGCCGAGAGGAAGATATCGACATTGTATATATGGCGTTTAAAGTATTTGACATGAATGACGATGGCTTCATTTCTTGCGAGGATCTCCAAAACGTGTTGTCGAGACTAGAGTTTTGGGACGCAAATAGAAGTGATATTGATAGCAAAAGTATGATTCGAGCTTATGACACCAATTTGGATGGGAAGCTCGATTTCGAAGAGTTTAAGAACATGATGTTGTTGACAAGCTCTTGATTATATTCGCCTTTGAGCTTTTAATGTTGCTTTGTTTGAAATGTGTTGTGTTGTGTGTTTTCTGTCCAGGTGGTTTCATTGGAGTTTTTGATATATGTCTTGAATAAACTAACCTAGAAAATTTTGAGTTAGGACTATATTTAACATGTTAAATATGAGTTTACTCCGTTAGTTAGATTAGAAACTTAACCTAATTAAAAGTATATGAGGGTGGCTTTGAAGAATAAAGGGG

mRNA sequence

TATATTTCTAAGCCAAAGAACCAAAAATTACCATGTCTCCTATTAGCTCCAACGACTTGCTCAGGATCTTCAACAAGCTCGACAAGAACCACGATGGCCTCATTTGCCTACACGAGCTCAACTGGCTCCTCCACACCATTGGCATCCAACTAACCATGGAGGAGTTGGAGTCTTTCCTCGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTACGAATCGATATCGAAGCAAAACAATGCCGAGTGCAAGGGAGGCGTAGGCGATGACGACGATGCAGGCCGAGAGGAAGATATCGACATTGTATATATGGCGTTTAAAGTATTTGACATGAATGACGATGGCTTCATTTCTTGCGAGGATCTCCAAAACGTGTTGTCGAGACTAGAGTTTTGGGACGCAAATAGAAGTGATATTGATAGCAAAAGTATGATTCGAGCTTATGACACCAATTTGGATGGGAAGCTCGATTTCGAAGAGTTTAAGAACATGATGTTGTTGACAAGCTCTTGATTATATTCGCCTTTGAGCTTTTAATGTTGCTTTGTTTGAAATGTGTTGTGTTGTGTGTTTTCTGTCCAGGTGGTTTCATTGGAGTTTTTGATATATGTCTTGAATAAACTAACCTAGAAAATTTTGAGTTAGGACTATATTTAACATGTTAAATATGAGTTTACTCCGTTAGTTAGATTAGAAACTTAACCTAATTAAAAGTATATGAGGGTGGCTTTGAAGAATAAAGGGG

Coding sequence (CDS)

ATGTCTCCTATTAGCTCCAACGACTTGCTCAGGATCTTCAACAAGCTCGACAAGAACCACGATGGCCTCATTTGCCTACACGAGCTCAACTGGCTCCTCCACACCATTGGCATCCAACTAACCATGGAGGAGTTGGAGTCTTTCCTCGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTACGAATCGATATCGAAGCAAAACAATGCCGAGTGCAAGGGAGGCGTAGGCGATGACGACGATGCAGGCCGAGAGGAAGATATCGACATTGTATATATGGCGTTTAAAGTATTTGACATGAATGACGATGGCTTCATTTCTTGCGAGGATCTCCAAAACGTGTTGTCGAGACTAGAGTTTTGGGACGCAAATAGAAGTGATATTGATAGCAAAAGTATGATTCGAGCTTATGACACCAATTTGGATGGGAAGCTCGATTTCGAAGAGTTTAAGAACATGATGTTGTTGACAAGCTCTTGA

Protein sequence

MSPISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEFLFFYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS

Homology

[Close]

BLAST of Tan0003473.1 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 6.4e-26
Identity = 72/162 (44.44%), Postives = 99/162 (61.11%), Query Frame = 0


Query: 4   ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIG-IQLTMEELESFLEKPSLDFNEFL-F 63
           I++NDL R+F  LDKN DGL+ L EL W+L  +G  + T +ELE  + K SLD +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  FYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLE 123
           +Y+++     ++    V  D+D       + +  AF VFD+N DG+IS E+L++VL RL 
Sbjct: 66  YYDAVLDSKGSKKNIDVVADND-------EAIARAFNVFDVNGDGYISAEELRDVLERLG 125

Query: 124 F------WDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMML 158
           F      WD  R       MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 FEEEAKAWDCGR-------MIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Tan0003473.1 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.4e-18
Identity = 58/155 (37.42%), Postives = 85/155 (54.84%), Query Frame = 0


Query: 9   LLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLE------KPSLDFNEFLFF 68
           L RIF+  DKN DG I + EL+  L  +G+   + +L+S +E         L+F++F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQ-NNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLE 128
           ++++        C GG  +DD +   E+   +  AFKVFD N DGFIS  +LQ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 129 FWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
             +    +   K MI + D N DG++DF EFKNMM
Sbjct: 150 LPEGGEMERVEK-MIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Tan0003473.1 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.0e-16
Identity = 59/161 (36.65%), Postives = 92/161 (57.14%), Query Frame = 0


Query: 9   LLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLE------KPSLDFNEFLFF 68
           L RIF+  DKN DG+I + EL+  L+ +G++  + ELES ++         L F +F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKQNNAECKGGVGDDDDAG-------REEDIDIVYMAFKVFDMNDDGFISCEDLQN 128
           ++S++    A   GG  +DD+          +E+ D  +  FKVFD + DG+IS  +LQ 
Sbjct: 101 HQSLNDSYFA--YGGEDEDDNEEDMRKSILSQEEAD-SFGGFKVFDEDGDGYISARELQM 160

Query: 129 VLSRLEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
           VL +L F + +  D   K MI + D+N DG++DF EFK+MM
Sbjct: 161 VLGKLGFSEGSEIDRVEK-MIVSVDSNRDGRVDFFEFKDMM 197

[Close]

BLAST of Tan0003473.1 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.9e-15
Identity = 59/170 (34.71%), Postives = 84/170 (49.41%), Query Frame = 0


Query: 4   ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEK------PSLDFN 63
           + S +L ++F   DKN DG I   EL       GI +  +EL++ ++K        +D  
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYESISKQNNA-----------ECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDG 123
           EF   Y SI   + A             +GG  DD+D G  E       AF VFD N DG
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMRE-------AFNVFDQNGDG 120

Query: 124 FISCEDLQNVLSRLEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
           FI+ ++L++VLS L       +D D + MI   D + DG++DF+EFK MM
Sbjct: 121 FITVDELRSVLSSLGLKHGRTAD-DCRRMISMVDADGDGRVDFKEFKQMM 162

[Close]

BLAST of Tan0003473.1 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.1e-15
Identity = 55/154 (35.71%), Postives = 85/154 (55.19%), Query Frame = 0


Query: 9   LLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFL------EKPSLDFNEFLFF 68
           L R+F+  DKN+DG I + EL+  L  +G+     +L+S +      +K  L F++F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLEF 128
           ++++ +       GG G   D   E D++    AF VFD + DGFIS  +LQ VL +L  
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
            +A   +   + MI + D+N DG++DF EFKNMM
Sbjct: 149 PEAGEIE-QVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of Tan0003473.1 vs. NCBI nr
Match: XP_022989807.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 266.5 bits (680), Expect = 1.4e-67
Identity = 134/162 (82.72%), Postives = 144/162 (88.89%), Query Frame = 0


Query: 1   MSP-ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEF 60
           MSP I+ NDLLRIFNKLDKN DGLICLHEL+WLL TIG++LTME+L SFLEKPSLDFNEF
Sbjct: 1   MSPIININDLLRIFNKLDKNRDGLICLHELSWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSR 120
           LFFYESISKQN  ECKGGV D +   RE+D+DIVYMAFKVFDMNDDGFISCE+LQ+VLSR
Sbjct: 61  LFFYESISKQNKGECKGGVSDVN--AREDDVDIVYMAFKVFDMNDDGFISCEELQHVLSR 120

Query: 121 LEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 162
           LE WD NRS ID KSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 LEIWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Tan0003473.1 vs. NCBI nr
Match: KAG6602129.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 266.5 bits (680), Expect = 1.4e-67
Identity = 135/162 (83.33%), Postives = 142/162 (87.65%), Query Frame = 0


Query: 1   MSP-ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEF 60
           MSP IS NDLLRIFNKLDKN DGLICLHELNWLL TIG++LTME+L SFLEKPSLDFNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSR 120
           LFFYESISKQN  ECKGGV D +   REED+DIVYMAFKVFDMNDDGFISCE+LQ VLSR
Sbjct: 61  LFFYESISKQNKGECKGGVSDVN--AREEDVDIVYMAFKVFDMNDDGFISCEELQLVLSR 120

Query: 121 LEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 162
           LE WD N +D D KSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 LELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Tan0003473.1 vs. NCBI nr
Match: XP_022959484.1 (probable calcium-binding protein CML44 [Cucurbita moschata])

HSP 1 Score: 263.8 bits (673), Expect = 9.4e-67
Identity = 133/162 (82.10%), Postives = 141/162 (87.04%), Query Frame = 0


Query: 1   MSP-ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEF 60
           MSP IS NDLLRIFNKLDKN DGLICLHELNWLL TIG++LTME+L SFLEKPSLDFNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSR 120
           LFFYESISKQN  ECKGGV D +   REED+D+VYMAFKVFDMNDDGFISCE+LQ VLSR
Sbjct: 61  LFFYESISKQNKGECKGGVSDVN--AREEDVDVVYMAFKVFDMNDDGFISCEELQLVLSR 120

Query: 121 LEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 162
           LE WD N +D D KSMIRAYDTNLDG LDFEEFKNMM L SS
Sbjct: 121 LELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

[Close]

BLAST of Tan0003473.1 vs. NCBI nr
Match: XP_022141411.1 (probable calcium-binding protein CML44 [Momordica charantia])

HSP 1 Score: 261.9 bits (668), Expect = 3.6e-66
Identity = 131/166 (78.92%), Postives = 144/166 (86.75%), Query Frame = 0


Query: 1   MSPISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEFL 60
           MSPI SNDLL+IFNKLDKN DGL+CL EL+WLL  IGIQLTMEELES LEKPSLDFNEFL
Sbjct: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60

Query: 61  FFYESISKQNNAECKGGVGDD-----DDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQN 120
           FFYESISK+N  EC GGVGDD     D    EED+DIVYMAFKVFDMNDDGFISCE+L++
Sbjct: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120

Query: 121 VLSRLEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 162
           VL RLE WD+NRSD + ++MIRAYDTNLDGKLDFEEFKNMMLLT+S
Sbjct: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

[Close]

BLAST of Tan0003473.1 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 259.6 bits (662), Expect = 1.8e-65
Identity = 127/158 (80.38%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEFLFF 62
           PISSNDLLRIFN+LDKN DGLIC+ EL WLL TIGIQLTMEELESFLEKPSLDF+EFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLEF 122
           YESISKQN  ECKGG+  D+    EED++IVYMAFKVFD+N DGFISCE+L+NVLSRLE 
Sbjct: 64  YESISKQNKGECKGGL-VDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTS 161
           WDANRS++D +SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 124 WDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Tan0003473.1 vs. ExPASy TrEMBL
Match: A0A6J1JGU4 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111486883 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 7.0e-68
Identity = 134/162 (82.72%), Postives = 144/162 (88.89%), Query Frame = 0


Query: 1   MSP-ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEF 60
           MSP I+ NDLLRIFNKLDKN DGLICLHEL+WLL TIG++LTME+L SFLEKPSLDFNEF
Sbjct: 1   MSPIININDLLRIFNKLDKNRDGLICLHELSWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSR 120
           LFFYESISKQN  ECKGGV D +   RE+D+DIVYMAFKVFDMNDDGFISCE+LQ+VLSR
Sbjct: 61  LFFYESISKQNKGECKGGVSDVN--AREDDVDIVYMAFKVFDMNDDGFISCEELQHVLSR 120

Query: 121 LEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 162
           LE WD NRS ID KSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 LEIWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Tan0003473.1 vs. ExPASy TrEMBL
Match: A0A6J1H868 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111460440 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.5e-67
Identity = 133/162 (82.10%), Postives = 141/162 (87.04%), Query Frame = 0


Query: 1   MSP-ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEF 60
           MSP IS NDLLRIFNKLDKN DGLICLHELNWLL TIG++LTME+L SFLEKPSLDFNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSR 120
           LFFYESISKQN  ECKGGV D +   REED+D+VYMAFKVFDMNDDGFISCE+LQ VLSR
Sbjct: 61  LFFYESISKQNKGECKGGVSDVN--AREEDVDVVYMAFKVFDMNDDGFISCEELQLVLSR 120

Query: 121 LEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 162
           LE WD N +D D KSMIRAYDTNLDG LDFEEFKNMM L SS
Sbjct: 121 LELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

[Close]

BLAST of Tan0003473.1 vs. ExPASy TrEMBL
Match: A0A6J1CJ36 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111011820 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.7e-66
Identity = 131/166 (78.92%), Postives = 144/166 (86.75%), Query Frame = 0


Query: 1   MSPISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEFL 60
           MSPI SNDLL+IFNKLDKN DGL+CL EL+WLL  IGIQLTMEELES LEKPSLDFNEFL
Sbjct: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60

Query: 61  FFYESISKQNNAECKGGVGDD-----DDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQN 120
           FFYESISK+N  EC GGVGDD     D    EED+DIVYMAFKVFDMNDDGFISCE+L++
Sbjct: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120

Query: 121 VLSRLEFWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 162
           VL RLE WD+NRSD + ++MIRAYDTNLDGKLDFEEFKNMMLLT+S
Sbjct: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

[Close]

BLAST of Tan0003473.1 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.9e-65
Identity = 126/158 (79.75%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEFLFF 62
           PISSNDLLRIFN+LDKN DGLIC+ EL WLL TIGIQLTMEELESFLEKPSLDF+EFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLEF 122
           YESISKQN  ECKGG+  D+    EED++IVYMAFKVFD+N DGFISCE+L+NVLSRLE 
Sbjct: 64  YESISKQNKGECKGGL-VDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTS 161
           WDAN+S++D +SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 124 WDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Tan0003473.1 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.1e-64
Identity = 124/158 (78.48%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSLDFNEFLFF 62
           PISSNDLLRIFN+LDKN DGLIC+ EL WLL TIGIQLTMEELESFLEKPSLDF+EFL F
Sbjct: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63

Query: 63  YESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLEF 122
           YES+SKQN  ECKGG+ D+ +   +ED++IVYMAFKVFD+N DGFISCE+L+NVLSRLE 
Sbjct: 64  YESMSKQNKGECKGGLVDEHE-DEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMMLLTS 161
           WDANRS++D +SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 124 WDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Tan0003473.1 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 118.6 bits (296), Expect = 4.5e-27
Identity = 72/162 (44.44%), Postives = 99/162 (61.11%), Query Frame = 0


Query: 4   ISSNDLLRIFNKLDKNHDGLICLHELNWLLHTIG-IQLTMEELESFLEKPSLDFNEFL-F 63
           I++NDL R+F  LDKN DGL+ L EL W+L  +G  + T +ELE  + K SLD +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  FYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLE 123
           +Y+++     ++    V  D+D       + +  AF VFD+N DG+IS E+L++VL RL 
Sbjct: 66  YYDAVLDSKGSKKNIDVVADND-------EAIARAFNVFDVNGDGYISAEELRDVLERLG 125

Query: 124 F------WDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMML 158
           F      WD  R       MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 FEEEAKAWDCGR-------MIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Tan0003473.1 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 91.7 bits (226), Expect = 5.9e-19
Identity = 58/155 (37.42%), Postives = 85/155 (54.84%), Query Frame = 0


Query: 9   LLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLE------KPSLDFNEFLFF 68
           L RIF+  DKN DG I + EL+  L  +G+   + +L+S +E         L+F++F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQ-NNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLE 128
           ++++        C GG  +DD +   E+   +  AFKVFD N DGFIS  +LQ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 129 FWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
             +    +   K MI + D N DG++DF EFKNMM
Sbjct: 150 LPEGGEMERVEK-MIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Tan0003473.1 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 82.4 bits (202), Expect = 3.6e-16
Identity = 55/154 (35.71%), Postives = 85/154 (55.19%), Query Frame = 0


Query: 9   LLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFL------EKPSLDFNEFLFF 68
           L R+F+  DKN+DG I + EL+  L  +G+     +L+S +      +K  L F++F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLEF 128
           ++++ +       GG G   D   E D++    AF VFD + DGFIS  +LQ VL +L  
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
            +A   +   + MI + D+N DG++DF EFKNMM
Sbjct: 149 PEAGEIE-QVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of Tan0003473.1 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 76.3 bits (186), Expect = 2.6e-14
Identity = 54/155 (34.84%), Postives = 81/155 (52.26%), Query Frame = 0


Query: 8   DLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEK------PSLDFNEFLF 67
           +L R+F   DKN DG I   EL+  L ++GI +  +EL   +EK        +D +EF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLE 127
            Y++I              D++   EED+     AF VFD N DGFI+ ++L+ VLS L 
Sbjct: 65  LYKTIM-------------DEEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVLSSLG 124

Query: 128 FWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
                  D D K MI+  D + DG+++++EF+ MM
Sbjct: 125 LKQGKTLD-DCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of Tan0003473.1 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 76.3 bits (186), Expect = 2.6e-14
Identity = 56/155 (36.13%), Postives = 80/155 (51.61%), Query Frame = 0


Query: 8   DLLRIFNKLDKNHDGLICLHELNWLLHTIGIQLTMEELESFLEKPSL------DFNEFLF 67
           +L RIF   D+N DG I   ELN  L  +GI +  ++L   +EK  L      D  EF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 68  FYESISKQNNAECKGGVGDDDDAGREEDIDIVYMAFKVFDMNDDGFISCEDLQNVLSRLE 127
            Y++I ++          D+++  RE        AF VFD N DGFI+ E+L++VL+ L 
Sbjct: 65  LYQTIMEER---------DEEEDMRE--------AFNVFDQNRDGFITVEELRSVLASLG 124

Query: 128 FWDANRSDIDSKSMIRAYDTNLDGKLDFEEFKNMM 157
                R+  D K MI   D + DG ++F+EFK MM
Sbjct: 125 L-KQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of Tan0003473.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	6.4e-26	44.44	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9SVG9	8.4e-18	37.42	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
P43187	6.0e-16	36.65	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
Q2QVI1	3.9e-15	34.71	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FI19	5.1e-15	35.71	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]

BLAST of Tan0003473.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022989807.1	1.4e-67	82.72	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]
KAG6602129.1	1.4e-67	83.33	putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022959484.1	9.4e-67	82.10	probable calcium-binding protein CML44 [Cucurbita moschata]	[more]
XP_022141411.1	3.6e-66	78.92	probable calcium-binding protein CML44 [Momordica charantia]	[more]
XP_023551499.1	1.8e-65	80.38	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0003473.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JGU4	7.0e-68	82.72	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114868...	[more]
A0A6J1H868	4.5e-67	82.10	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1CJ36	1.7e-66	78.92	probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1FLU5	1.9e-65	79.75	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1K1Y7	2.1e-64	78.48	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893...	[more]

BLAST of Tan0003473.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	4.5e-27	44.44	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	5.9e-19	37.42	calmodulin like 42	[more]
AT5G44460.1	3.6e-16	35.71	calmodulin like 43	[more]
AT1G05990.1	2.6e-14	34.84	EF hand calcium-binding protein family	[more]
AT3G07490.1	2.6e-14	36.13	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 8..36 e-value: 0.014 score: 24.6 coord: 131..159 e-value: 0.02 score: 24.0 coord: 92..120 e-value: 0.78 score: 18.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 94..156 e-value: 1.2E-8 score: 35.2
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 12..29 e-value: 5.4E-4 score: 19.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..161 score: 10.97069
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 11.723866
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 11.863342
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 96..156 e-value: 9.69056E-11 score: 52.5501
None	No IPR available	GENE3D	1.10.238.10		coord: 5..69 e-value: 3.7E-9 score: 38.5
None	No IPR available	GENE3D	1.10.238.10		coord: 81..161 e-value: 1.5E-15 score: 59.1
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..161
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 1..161
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 6..158

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0003473	Tan0003473	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003473.1-three_prime_utr	Tan0003473.1-three_prime_utr-LG11:10451574..10451805	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003473.1-exon	Tan0003473.1-exon-LG11:10451574..10452323	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003473.1-cds	Tan0003473.1-cds-LG11:10451806..10452291	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0003473.1-five_prime_utr	Tan0003473.1-five_prime_utr-LG11:10452292..10452323	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0003473.1	Tan0003473.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding