Tan0000948.1 (mRNA) Snake gourd v1

Overview


Name	Tan0000948.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	beta-amyrin 11-oxidase-like
Location	LG01: 25380259 .. 25381519 (-)
Sequence length	504
RNA-Seq Expression	Tan0000948.1
Synteny	Tan0000948.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAGCCCTCAAGGCCGAGCACAGGCGACTGCGGTGGCTGACAACGGCGACGATCAGCGGCCACGCGGCGCTTCAGATGTATATCAATGATATTGAACAGACAGTGATGAATGGGTTAGAGGAATGGGCAAGCATGAAGAGGCCAATAGAGCTGTTGACAGAGATAAAGAAGCTGACCTTCAAAGTTATTTGGAACATTTTCATGGGTTCAACTGTTGTTGGCTCTTCAATGGAGGGTTTGTTTCATGAGGTTGCTACTGGATTTTTCTTGCTTCCCATTAATTTTCCTGCCTTCTGCTTCCATAAATCCTTCAAGGTAACCAAGTTTTCTTTAACATATAATATTGTTGGTTTAAATCTTATTTTAAACCTCCAACATTTTTTTTTTTTAAATATTGCATCATGCATGAATATGTTGATAGGAGTTTGAACTTATCGGCCCTTTTTAAAAAAGGTAGAATATGCACATGACCTTTAATTTTAAGCACTGAGTTATGTTTAGAATGGCTTACAACTAACTCTCAACTTCTATATAATAATCTTCATTTACATAATTGATAATCATAATTAGCTTCTTTTTTAAAACTTTAGAGGGTCATTCATGTTGATTTACATAATAATACTATTACTGTAGAGTGAATAAGTATAATTCATCGGTTAAGACATCTACTTTTCCTTTTGAAGTCGATGGTTCAAAGATTTACCAACACATTTTATTGTACTAAAAAAAACTATCGACCTAGTAGTTAGTAGTAATACATATTTTTATAGAAAAAACACTTGTTGTGCTCCCGACTTGCTAGTCACACAATTTTTAAACTAATGGTTAAAAAAGGAGTTGAGCAATACCCAAGTTGTGCTCTATTTTTATAATGAATACAAATTTTTATCTTTGGTTATATTTACAACTACTTCTTGCGCTGTTATATAACTCTAAGCATTTAATTATACGATATTCACCATTAATCTTTCTCGTTACACACAAATATATAGGCTCGAAAAAGATTATCGTGAATGAGAAGAGAATGGTGAAGGAAAGCAAAGGAAGAAATTGGGAAGCAAAAGATATGATGGATTTGCTGATAGAAGTGAGAGATGAAGATGGTGAAGGGATGGATGATGAGACCATTATAGATTTGATTTTTGGTAAGTTATTTGCAGGCCATGAGACTTCAGCTTACACTACAATGTGGGCTGTTTTGTTTCTTACAAATCATCCACGCATATATTCAAAAGACCAAGGTAAGTGTTATTGTTAA

mRNA sequence

ATGCAAGCCCTCAAGGCCGAGCACAGGCGACTGCGGTGGCTGACAACGGCGACGATCAGCGGCCACGCGGCGCTTCAGATGTATATCAATGATATTGAACAGACAGTGATGAATGGGTTAGAGGAATGGGCAAGCATGAAGAGGCCAATAGAGCTGTTGACAGAGATAAAGAAGCTGACCTTCAAAGTTATTTGGAACATTTTCATGGGTTCAACTGTTGTTGGCTCTTCAATGGAGGGCTCGAAAAAGATTATCGTGAATGAGAAGAGAATGGTGAAGGAAAGCAAAGGAAGAAATTGGGAAGCAAAAGATATGATGGATTTGCTGATAGAAGTGAGAGATGAAGATGGTGAAGGGATGGATGATGAGACCATTATAGATTTGATTTTTGGTAAGTTATTTGCAGGCCATGAGACTTCAGCTTACACTACAATGTGGGCTGTTTTGTTTCTTACAAATCATCCACGCATATATTCAAAAGACCAAGGTAAGTGTTATTGTTAA

Coding sequence (CDS)

ATGCAAGCCCTCAAGGCCGAGCACAGGCGACTGCGGTGGCTGACAACGGCGACGATCAGCGGCCACGCGGCGCTTCAGATGTATATCAATGATATTGAACAGACAGTGATGAATGGGTTAGAGGAATGGGCAAGCATGAAGAGGCCAATAGAGCTGTTGACAGAGATAAAGAAGCTGACCTTCAAAGTTATTTGGAACATTTTCATGGGTTCAACTGTTGTTGGCTCTTCAATGGAGGGCTCGAAAAAGATTATCGTGAATGAGAAGAGAATGGTGAAGGAAAGCAAAGGAAGAAATTGGGAAGCAAAAGATATGATGGATTTGCTGATAGAAGTGAGAGATGAAGATGGTGAAGGGATGGATGATGAGACCATTATAGATTTGATTTTTGGTAAGTTATTTGCAGGCCATGAGACTTCAGCTTACACTACAATGTGGGCTGTTTTGTTTCTTACAAATCATCCACGCATATATTCAAAAGACCAAGGTAAGTGTTATTGTTAA

Protein sequence

MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIWNIFMGSTVVGSSMEGSKKIIVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLFLTNHPRIYSKDQGKCYC

Homology

[Close]

BLAST of Tan0000948.1 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.5e-30
Identity = 77/191 (40.31%), Postives = 114/191 (59.69%), Query Frame = 0


Query: 6   AEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIW 65
           AEHR  R L T+ I GH AL MY+  +E+ V+N LEE +SMK P+ELL E+KK++FK I 
Sbjct: 136 AEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIV 195

Query: 66  NIFMGST------VVGSS-----------------------MEGSKKI------IVNEKR 125
           ++FMGS+       +GSS                       +E  KK+      +V+E+R
Sbjct: 196 HVFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVDERR 255

Query: 126 -MVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVL 161
            M++       + KD++D+L+EV+DE+G  ++DE I DL+ G LFAGHE++A + MW++ 
Sbjct: 256 LMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMWSIT 315

[Close]

BLAST of Tan0000948.1 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-23
Identity = 71/190 (37.37%), Postives = 101/190 (53.16%), Query Frame = 0


Query: 7   EHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIWN 66
           EH+RLR LT+A ++G  AL +YI  IE+TV   LE+W+ M   IE L+ ++KLTFKVI  
Sbjct: 138 EHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMY 197

Query: 67  IFM--------------------GSTVVGSSMEG----------------SKKIIVNEKR 126
           IF+                    G   +G ++ G                 + I+ N + 
Sbjct: 198 IFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRN 257

Query: 127 MVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLF 161
             K++   N   KDM+D LI+V+DE+G  +DDE IIDL+   L AGHE+S + TMWA + 
Sbjct: 258 QRKQNISSN--RKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATIL 317

[Close]

BLAST of Tan0000948.1 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.5e-22
Identity = 64/188 (34.04%), Postives = 98/188 (52.13%), Query Frame = 0


Query: 7   EHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIWN 66
           EH+RLR LT A ++GH AL  YI  IE+ V+  L++W  M    E LT ++KLTF++I  
Sbjct: 139 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMY 198

Query: 67  IFMGST-----------------------------VVGSSMEGSKKIIVNEKRMVKESKG 126
           IF+ S                                  +++  K ++   + +V E + 
Sbjct: 199 IFLSSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRN 258

Query: 127 RNWE-----AKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLFLT 161
           +  +      KDM+D L+ V+DEDG+ +DDE IID++   L AGHE+S +T MWA +FL 
Sbjct: 259 QRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQ 318

[Close]

BLAST of Tan0000948.1 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.3e-21
Identity = 63/188 (33.51%), Postives = 98/188 (52.13%), Query Frame = 0


Query: 7   EHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIWN 66
           EHRR+R LT A I+G  AL  Y+  I++TV + L  WA     +E LTE++++TFK+I  
Sbjct: 166 EHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQ 225

Query: 67  IFMGSTVVGSSME----------GSKKIIVN------------------------EKRMV 126
           IF+G     ++            G + + +N                        ++R  
Sbjct: 226 IFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDERRA 285

Query: 127 KESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLFLT 161
             +KG +    DMMD LIE +DE G  +DD+ IID++   L AGHE+S + TMWA +FL 
Sbjct: 286 ARAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQ 345

[Close]

BLAST of Tan0000948.1 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 9.3e-20
Identity = 65/190 (34.21%), Postives = 101/190 (53.16%), Query Frame = 0


Query: 7   EHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKR-PIELLTEIKKLTFKVIW 66
           +HRR+R LT A I+G  AL  Y++ I+QTV+  L  W+S +   +E LTE++++TFK+I 
Sbjct: 149 DHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKIIV 208

Query: 67  NIFM--------------------GSTVVGSSMEG---------SKKII-----VNEKRM 126
            IFM                    G   +  ++ G          +K++     V + R 
Sbjct: 209 QIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGVLDGRR 268

Query: 127 VKESKG-RNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLF 161
              +KG +   A DMMD LIE  DE G  + D+ I+D++   L AGHE+S + TMWA +F
Sbjct: 269 AAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITMWATVF 328

[Close]

BLAST of Tan0000948.1 vs. NCBI nr
Match: XP_011650352.2 (beta-amyrin 11-oxidase [Cucumis sativus] >KAE8650035.1 hypothetical protein Csa_011096 [Cucumis sativus])

HSP 1 Score: 231.9 bits (590), Expect = 4.1e-57
Identity = 123/196 (62.76%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LTTA ISGH+AL+MYIN IEQTV++GLEEWASMKRPIELLTEIKKLT
Sbjct: 128 MQVFKAEHRRLRRLTTAPISGHSALEMYINHIEQTVISGLEEWASMKRPIELLTEIKKLT 187

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 188 FKIIWNIFMGSSSIDSSIGEIEALFAKITLGFISLPINLPGFSFYKSLKARKELLTILQS 247

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G  WEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHETSAYT 
Sbjct: 248 IINQKRMAKKREGEGWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 307

[Close]

BLAST of Tan0000948.1 vs. NCBI nr
Match: XP_022145397.1 (beta-amyrin 11-oxidase-like, partial [Momordica charantia])

HSP 1 Score: 229.2 bits (583), Expect = 2.7e-56
Identity = 121/196 (61.73%), Postives = 144/196 (73.47%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           M+  KAEHRRLR LTTA +SGH AL+MYIN IE+TV++GLEEWASM+RP+ELLTEIK+LT
Sbjct: 123 MRVSKAEHRRLRRLTTAPVSGHRALEMYINHIEETVIDGLEEWASMQRPMELLTEIKELT 182

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FKVIWNIFMGST +GS                              S++  K++      
Sbjct: 183 FKVIWNIFMGSTSIGSCMREMEALFYDISLGFFSLPINFPGFHFHKSLKARKRLLEILQS 242

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           IV++KR VK++ G+NWEAKDMMDL+IEVRDEDGE +DDETIIDLIFGKLFAGHETSA T 
Sbjct: 243 IVSKKRFVKKNNGKNWEAKDMMDLMIEVRDEDGEELDDETIIDLIFGKLFAGHETSADTA 302

[Close]

BLAST of Tan0000948.1 vs. NCBI nr
Match: XP_008448712.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 229.2 bits (583), Expect = 2.7e-56
Identity = 121/196 (61.73%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LT A ISGH+AL+MYIN IEQTV++GLEEWASMK+PIELLTEIKKLT
Sbjct: 128 MQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELLTEIKKLT 187

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 188 FKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKELLSLLQS 247

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G NWEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHETSAYT 
Sbjct: 248 IINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 307

[Close]

BLAST of Tan0000948.1 vs. NCBI nr
Match: KAA0053060.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa] >TYK11515.1 beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 229.2 bits (583), Expect = 2.7e-56
Identity = 121/196 (61.73%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LT A ISGH+AL+MYIN IEQTV++GLEEWASMK+PIELLTEIKKLT
Sbjct: 129 MQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELLTEIKKLT 188

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 189 FKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKELLSLLQS 248

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G NWEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHETSAYT 
Sbjct: 249 IINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 308

[Close]

BLAST of Tan0000948.1 vs. NCBI nr
Match: XP_008448713.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 229.2 bits (583), Expect = 2.7e-56
Identity = 121/196 (61.73%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LT A ISGH+AL+MYIN IEQTV++GLEEWASMK+PIELLTEIKKLT
Sbjct: 128 MQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELLTEIKKLT 187

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 188 FKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKELLSLLQS 247

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G NWEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHETSAYT 
Sbjct: 248 IINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 307

[Close]

BLAST of Tan0000948.1 vs. ExPASy TrEMBL
Match: A0A0A0L1B5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G011860 PE=3 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 4.4e-57
Identity = 122/196 (62.24%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LTTA ISGH+AL+MYIN IEQTV++GLEEWASMKRPIELLTEIKKLT
Sbjct: 48  MQVFKAEHRRLRRLTTAPISGHSALEMYINHIEQTVISGLEEWASMKRPIELLTEIKKLT 107

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 108 FKIIWNIFMGSSSIDSSIGEIEALFAKITLGFISLPINLPGFSFYKSLKARKELLTILQS 167

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G  WEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHET+AYT 
Sbjct: 168 IINQKRMAKKREGEGWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETTAYTA 227

[Close]

BLAST of Tan0000948.1 vs. ExPASy TrEMBL
Match: A0A5D3CIW4 (Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001790 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.3e-56
Identity = 121/196 (61.73%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LT A ISGH+AL+MYIN IEQTV++GLEEWASMK+PIELLTEIKKLT
Sbjct: 129 MQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELLTEIKKLT 188

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 189 FKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKELLSLLQS 248

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G NWEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHETSAYT 
Sbjct: 249 IINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 308

[Close]

BLAST of Tan0000948.1 vs. ExPASy TrEMBL
Match: A0A6J1CWG2 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014855 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.3e-56
Identity = 121/196 (61.73%), Postives = 144/196 (73.47%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           M+  KAEHRRLR LTTA +SGH AL+MYIN IE+TV++GLEEWASM+RP+ELLTEIK+LT
Sbjct: 123 MRVSKAEHRRLRRLTTAPVSGHRALEMYINHIEETVIDGLEEWASMQRPMELLTEIKELT 182

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FKVIWNIFMGST +GS                              S++  K++      
Sbjct: 183 FKVIWNIFMGSTSIGSCMREMEALFYDISLGFFSLPINFPGFHFHKSLKARKRLLEILQS 242

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           IV++KR VK++ G+NWEAKDMMDL+IEVRDEDGE +DDETIIDLIFGKLFAGHETSA T 
Sbjct: 243 IVSKKRFVKKNNGKNWEAKDMMDLMIEVRDEDGEELDDETIIDLIFGKLFAGHETSADTA 302

[Close]

BLAST of Tan0000948.1 vs. ExPASy TrEMBL
Match: A0A1S3BJR1 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.3e-56
Identity = 121/196 (61.73%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LT A ISGH+AL+MYIN IEQTV++GLEEWASMK+PIELLTEIKKLT
Sbjct: 128 MQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELLTEIKKLT 187

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 188 FKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKELLSLLQS 247

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G NWEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHETSAYT 
Sbjct: 248 IINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 307

[Close]

BLAST of Tan0000948.1 vs. ExPASy TrEMBL
Match: A0A1S3BKD0 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.3e-56
Identity = 121/196 (61.73%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 1   MQALKAEHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLT 60
           MQ  KAEHRRLR LT A ISGH+AL+MYIN IEQTV++GLEEWASMK+PIELLTEIKKLT
Sbjct: 128 MQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPIELLTEIKKLT 187

Query: 61  FKVIWNIFMGSTVVGS------------------------------SMEGSKKI------ 120
           FK+IWNIFMGS+ + S                              S++  K++      
Sbjct: 188 FKIIWNIFMGSSSIDSSIGEIEALFAQIALGFISLPINFPGFSFHKSLKARKELLSLLQS 247

Query: 121 IVNEKRMVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTT 161
           I+N+KRM K+ +G NWEAKDMMDLLIE++DEDGE +DDETI DLIFGKLFAGHETSAYT 
Sbjct: 248 IINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 307

[Close]

BLAST of Tan0000948.1 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 110.9 bits (276), Expect = 9.8e-25
Identity = 71/190 (37.37%), Postives = 101/190 (53.16%), Query Frame = 0


Query: 7   EHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIWN 66
           EH+RLR LT+A ++G  AL +YI  IE+TV   LE+W+ M   IE L+ ++KLTFKVI  
Sbjct: 138 EHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMY 197

Query: 67  IFM--------------------GSTVVGSSMEG----------------SKKIIVNEKR 126
           IF+                    G   +G ++ G                 + I+ N + 
Sbjct: 198 IFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRN 257

Query: 127 MVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLF 161
             K++   N   KDM+D LI+V+DE+G  +DDE IIDL+   L AGHE+S + TMWA + 
Sbjct: 258 QRKQNISSN--RKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATIL 317

[Close]

BLAST of Tan0000948.1 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 110.9 bits (276), Expect = 9.8e-25
Identity = 71/190 (37.37%), Postives = 101/190 (53.16%), Query Frame = 0


Query: 7   EHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIWN 66
           EH+RLR LT+A ++G  AL +YI  IE+TV   LE+W+ M   IE L+ ++KLTFKVI  
Sbjct: 138 EHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMY 197

Query: 67  IFM--------------------GSTVVGSSMEG----------------SKKIIVNEKR 126
           IF+                    G   +G ++ G                 + I+ N + 
Sbjct: 198 IFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRN 257

Query: 127 MVKESKGRNWEAKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLF 161
             K++   N   KDM+D LI+V+DE+G  +DDE IIDL+   L AGHE+S + TMWA + 
Sbjct: 258 QRKQNISSN--RKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATIL 317

[Close]

BLAST of Tan0000948.1 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-23
Identity = 64/188 (34.04%), Postives = 98/188 (52.13%), Query Frame = 0


Query: 7   EHRRLRWLTTATISGHAALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKVIWN 66
           EH+RLR LT A ++GH AL  YI  IE+ V+  L++W  M    E LT ++KLTF++I  
Sbjct: 139 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMY 198

Query: 67  IFMGST-----------------------------VVGSSMEGSKKIIVNEKRMVKESKG 126
           IF+ S                                  +++  K ++   + +V E + 
Sbjct: 199 IFLSSESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRN 258

Query: 127 RNWE-----AKDMMDLLIEVRDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLFLT 161
           +  +      KDM+D L+ V+DEDG+ +DDE IID++   L AGHE+S +T MWA +FL 
Sbjct: 259 QRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQ 318

[Close]

BLAST of Tan0000948.1 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 45.1 bits (105), Expect = 6.6e-05
Identity = 36/165 (21.82%), Postives = 70/165 (42.42%), Query Frame = 0


Query: 23  AALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKV-IWNIF------------- 82
           +AL+  ++ IE  V+  L  W S K  I  L  +K+  F V I + F             
Sbjct: 153 SALRPTVSHIELLVLQTLSSWTSQK-SINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIK 212

Query: 83  ----------------MGSTVVGSSMEGSKKIIVNEKRMVKESKGRNWEAKDMMDLLIEV 142
                           +  T+   SM+   ++    ++++++ +    E   ++ +L+  
Sbjct: 213 LLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGA 272

Query: 143 RDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLFLTNHPRI 158
           +D+   G+ D  I D I G +FA  +T+A    W + +L +HP +
Sbjct: 273 KDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNL 316

[Close]

BLAST of Tan0000948.1 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 45.1 bits (105), Expect = 6.6e-05
Identity = 36/165 (21.82%), Postives = 70/165 (42.42%), Query Frame = 0


Query: 23  AALQMYINDIEQTVMNGLEEWASMKRPIELLTEIKKLTFKV-IWNIF------------- 82
           +AL+  ++ IE  V+  L  W S K  I  L  +K+  F V I + F             
Sbjct: 153 SALRPTVSHIELLVLQTLSSWTSQK-SINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIK 212

Query: 83  ----------------MGSTVVGSSMEGSKKIIVNEKRMVKESKGRNWEAKDMMDLLIEV 142
                           +  T+   SM+   ++    ++++++ +    E   ++ +L+  
Sbjct: 213 LLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGA 272

Query: 143 RDEDGEGMDDETIIDLIFGKLFAGHETSAYTTMWAVLFLTNHPRI 158
           +D+   G+ D  I D I G +FA  +T+A    W + +L +HP +
Sbjct: 273 KDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNL 316

The following BLAST results are available for this feature:

BLAST of Tan0000948.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.5e-30	40.31	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	1.4e-23	37.37	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	1.5e-22	34.04	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q43246	1.3e-21	33.51	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q5VRM7	9.3e-20	34.21	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]

BLAST of Tan0000948.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650352.2	4.1e-57	62.76	beta-amyrin 11-oxidase [Cucumis sativus] >KAE8650035.1 hypothetical protein Csa_...	[more]
XP_022145397.1	2.7e-56	61.73	beta-amyrin 11-oxidase-like, partial [Momordica charantia]	[more]
XP_008448712.1	2.7e-56	61.73	PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo]	[more]
KAA0053060.1	2.7e-56	61.73	beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa] >TYK11515.1 be...	[more]
XP_008448713.1	2.7e-56	61.73	PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo]	[more]

BLAST of Tan0000948.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1B5	4.4e-57	62.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G011860 PE=3 SV=1	[more]
A0A5D3CIW4	1.3e-56	61.73	Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1CWG2	1.3e-56	61.73	beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014855 PE=3 ...	[more]
A0A1S3BJR1	1.3e-56	61.73	beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 P...	[more]
A0A1S3BKD0	1.3e-56	61.73	beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490801 P...	[more]

BLAST of Tan0000948.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	9.8e-25	37.37	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	9.8e-25	37.37	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	1.1e-23	34.04	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G29090.1	6.6e-05	21.82	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	6.6e-05	21.82	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 85..160 e-value: 9.9E-11 score: 41.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..162 e-value: 8.9E-28 score: 99.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 6..160
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 84..160
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 3..74
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 84..160
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 3..74

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0000948	Tan0000948	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0000948.1-exon	Tan0000948.1-exon-LG01:25380259..25380524	exon
Tan0000948.1-exon	Tan0000948.1-exon-LG01:25381282..25381519	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0000948.1-cds	Tan0000948.1-cds-LG01:25380259..25380524	CDS
Tan0000948.1-cds	Tan0000948.1-cds-LG01:25381282..25381519	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0000948.1	Tan0000948.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen