Spg039596.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg039596.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	nifU-like protein 4, mitochondrial
Location	scaffold10: 41139083 .. 41143427 (-)
Sequence length	885
RNA-Seq Expression	Spg039596.1
Synteny	Spg039596.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCCAACCGAACCGAAGCTCAAACTTTGCGTCAGCACCACGCTGTACGTTAGGTATTTATCAAGAAGAACTCTTCACTTTGCAAGAACAACAGGGATAGAGGGAAGAATGAGGGGTCTAGGAAGTTTGATTGTTCGAGCTCGAAGAATTCCAAAATTGGATTCTCGAGGAGTTGGGAAGATCCGATTCTTCTCCGCCGCAACGACTTCTTCTCTATTCGGCGGTGAAGAAGCTGCTTCTTGCTCTGTGTCGTCGAGGACTTGCTCCGATTCTTACTTCTCCTGGAAATGGAACCACTTCGGAGGTGCGTAGGCTTTGACGAACGATCAATTGAGAAGTTTTACATCATGTGTGTTTTTGTATAATCCATCGTATTTTGTTGCATTCAGGACAGAGGAGAACAATGTTCATCGAAACTCAATCAACGCCGAATCCTTCGTCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCTGATTTTCCAAATGCGCGTTCGGCTATGAACTCCCCACTAGCAAAAGCCCTTTATGGCGTTGACGGTATCCATTCTGTATTTGTAGTATCTTTTTCGATTTCTGTTGGACTTTCAATCTGGAAACCCTTTTCCCTTTACCTGCATTTGAAGGAATTGTAACTGTATACCCGCATTTTCTCTCCCTTTGCCTTCCATTTTTCCAAGGTTCTACTTAACAATAACTATATTAATTTAAAACTCCACACCGCCTCTTTCAATCTTCCTTTTTATTGGTGATATTCCGATTCTGATTTCTGAGCATGAATTCCTTTAATTATCATTCAATTGCCTAAATATGAAGACTGGGTTCCAGCAAACATCTTAATATTTGAATGTTCAAAATGATACCGATATTGAGATTTTCTTTCGAGTGTACATGGGTTACTTTTCCTATTTTTCAATTTGCATTGCAAGTTTGAACTTAGTAGGGGGTTTCTTCTCTTTCTATTTGTAAATGTTATTTTCTAAACTGAAGTCAATTAAGTCATTAACATTATCTTTTATTTGACTGTGTTGATTGATTTATGTATTTAGTCTAAACATGAGCTACTGGTGTAATATGCTCACGATGAATAGCATGTAGATGTAGAAGGGTATAGGTGGACGAGGCCAAATATCTTCCAGTAGCTGTTCGACTAGAAAATTTATGTAATTATAGTTTTCCTATAGGTTCGATATTCTTACTTTTCATTTTCTAGTGCAGCCATATAAGATAAATAGAGTCCTGGAACCTCACCTTGTGCATAATAATTCTTTGATCTAAGTATTTGAAACTTGAATGGCTGGATACAAACATTGTTGATCACATTGATTTGATTTTTTAGTATTCGCGTCTTGCAAATTTGCAATTGAAATATCTTTATTTATCACTCAATAGCCAAGCTTATTATAGAAAAGTTGTGTGGCATAACAAATAAGTGCTATTAATCTAGGTCTAGGTTGTGGATGGTTACCTTACCAAAAATAACCATGCAATTTAATGATAATCTTTTCGCCTCATGTTTTCCATATGTAAGGACATTGTTCAATTGTTAGTTTGAATTTTAATTGTTACAATCACTTGGTGATGGTTTGCAAGTATTCTCGTTGGTTTTATTGATGCCTGGCATGAAGCTTACTTGATTGCTTGTGCAGGCGTTGTTCGAGTCTTCTTTGGATCAGATTTTGTTACTATAACAAAATCAAATGATGCTTCTTGGGATTTCCTAAAGCCCGAAATTTTTGCCGCAATCATGGATTTCTATTCTTCTGGGCAACCACTGTTTCTAGACTCCAAAACTGCTGCTGCCATGGACACAGCCATCAATGAAGTATGTCTTCTAAGTTCTAACCGCAAATTAATGCACTTTATTGTTAGATTTTTACTCATTAAGAAACAACTTAATTTTTTAAATCACATATTGTTCTCTTTTCTCTGAACTTTTTATATTAGTTAGTGTTCACCAGTCGATGCATTTACATATTTTCTCTTTTTTCTCCAGCGATGTTTATGATAATTTTATACTAGTTTGTGTTTTGATCTCATTCACTTCTATGATGTTCTTTTTCCTCTCTTGCTATACTCTACATGATGTAGATCCGACGAGAGTTCCTTGGTGAAACCACACTAGCATGTACATAGATTTTTAATTGTTCCTTAAATACTAATATAGATATATGAACATTTAACATCATCATACTTCAAATTAAAAGTACTTTTGAGGCTCCTAGTATTCATAGCATGATGTGGTGCCTTGCAGTTCTATTAACGGCAATGTTACTTCTATCGAAGACTTGTGCACCGTATTCTTATTGAAATTACTATATTTTAATCTTACTCCTTTGGATTCAACTTTGGCTAAAAACTTCTTTTCTGTACTCCATGTTGGTCTTTTTGATGGATATTCAATTTTCTTAGTCAAATTAGTAACCTGTTTTCTTTTTTCCATCATGTTGTTAAGAAATGGGATTTTCCATTAATACAGAGGAGGGATATACTAGCTACTATGCCACTTGGAGACCTAGAGAATTGAAAAGGTCCAGTTGTAGAATGAAATTCTCTTGTTAGTTCTTATTAAAATGAGTATGTCTACTTAAATTAAGTTCAGTTACTTATCTAAAAGATGAGTATGTCAGCGTCTTCTATGAGATGTCAATTTTTCTTTTGTAATATTTAATTTCGAAGTTTGACCTTTTGGTGATTGCTTGCCATGCTCTGCAAGTATTGAATCTTTCCTTAAAATGGTATATTTGTCATGTTGAAATTTGAAACATCATATATTTTTATTATTATTTTAGTAGTTTGATACAGATCAGTTCTTAATAGATTTGTCCGCTTGTGCTTACTTCTTAGGATGATTCTGAAACCGTTGCTATGATCAAAGAGCTGCTTGAGACCCGTATCCGACCAGCAGTGCAAGATGATGGCGGAGATATTGAATACCGAGGATATGATGAGTAGGTTTCTCTGAATTCAGTTAAAGCTTTAAAGCTGTTTCGTAAAGCATCAGGTGTTGTATGCATGAGTGTTTATATCTATTGAATTTTGCATTCCTGTCTTCTTCAGAGAAACTGGTATTGTCAAATTGAGAATGCAAGGAGCATGCAGTGGCTGTCCAAGTTCATCTGTCACTTTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAGGATTATACGATCTTTGTAAATTTGTAGTAACCTCATGTTTAGTTAGAAACATATCCAATTTTCCACTTGCACAGCTAGGTTGGTCCTCGTGAGCATTTTTGGCACAATTGCTCACACATATTGATTGCTTTTGTTTCCTGATATTTTATGCTGGCAGTATCTTTTATATGATTCCCGGAGTGTCTTACAAGCATATTTCTTATCTGCATTCTGCATGCTGGAAGTATTGAGCTTGAATACAAACCAGCTGGAGTGGAAGGACATTGAATCTTATTGTTGTGACCAAGGGCAAGAACAGTTGGTTTTCCAACTTCTTTATCCCTCTATAATAGTCAACTTCTTCTAGGCACCTTATTCACAGAGACTTGTTGTATTTAAGATAATTTGCTACTGTTCACGTTTTACTAAAAAAAAAAAATCATATGGAATGCAATATGCTCTAGTTGGATAACAAGAATGCATTGATGAACGGAGCATGCTTGATAGAAAGAAGGTGTTGCATCCTAGAGAGCCAATGTAGAGAATCAATTTTAAGGGAGAAAACAGAAATAGAACGATGTTTCAAAACTGCTTGGCCCAATTCTAATATCTAGGTCTTTTCCTCTTTTGTTGTTCTTATGCTTCTCAGAATTATATATATATATGTGTGTGTATATATACATACATTTGAATGTCTATTTTCAACTTATAGGTGAAGGGCGTGGAACAAGAATTTGACGTCGAGGAAGAGGATGTAAAGGCAACCAGCCAGATGGAGTAAAAGATGAGTTAATCGATTCACTCTTTGTTACATTGAGCCACAATACTGATCTGGAGGGTGACCCATACGAGTCGGTCAATTCATTCTTTGTTAGGTTGAGCCACAACACGGATCTTGAGGGGCGACCATGTGGGTACTTGTCAGAGAATAAAAAACCCGTTGAGGTTGTTAAATTATTTCATGGTTTATAGAGTAGAGTACCTTGTAACTTTTATTCCTAGCAAAGAGGAGAAGTGTGTGAATATTGAACCGTAACTGGGATTTCAAGGCCTTAGCATATTTTGGACACGGCGAAAGTATATGGTAATGTAAGAGACATTCGACTGTTCACAATAATAATATTTTTGTTCCATATCCTGGAATGTTAGAAACAATTCTTATATTTCTTGTAAAG

mRNA sequence

ATGGACCCAACCGAACCGAAGCTCAAACTTTGCGTCAGCACCACGCTGTACGTTAGGTATTTATCAAGAAGAACTCTTCACTTTGCAAGAACAACAGGGATAGAGGGAAGAATGAGGGGTCTAGGAAGTTTGATTGTTCGAGCTCGAAGAATTCCAAAATTGGATTCTCGAGGAGTTGGGAAGATCCGATTCTTCTCCGCCGCAACGACTTCTTCTCTATTCGGCGGTGAAGAAGCTGCTTCTTGCTCTGTGTCGTCGAGGACTTGCTCCGATTCTTACTTCTCCTGGAAATGGAACCACTTCGGAGGACAGAGGAGAACAATGTTCATCGAAACTCAATCAACGCCGAATCCTTCGTCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCTGATTTTCCAAATGCGCGTTCGGCTATGAACTCCCCACTAGCAAAAGCCCTTTATGGCGTTGACGGCGTTGTTCGAGTCTTCTTTGGATCAGATTTTGTTACTATAACAAAATCAAATGATGCTTCTTGGGATTTCCTAAAGCCCGAAATTTTTGCCGCAATCATGGATTTCTATTCTTCTGGGCAACCACTGTTTCTAGACTCCAAAACTGCTGCTGCCATGGACACAGCCATCAATGAAGATGATTCTGAAACCGTTGCTATGATCAAAGAGCTGCTTGAGACCCGTATCCGACCAGCAGTGCAAGATGATGGCGGAGATATTGAATACCGAGGATATGATGAAGAAACTGGTATTGTCAAATTGAGAATGCAAGGAGCATGCAGTGGCTGTCCAAGTTCATCTGTCACTTTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAGGATTATACGATCTTTGTAA

Coding sequence (CDS)

ATGGACCCAACCGAACCGAAGCTCAAACTTTGCGTCAGCACCACGCTGTACGTTAGGTATTTATCAAGAAGAACTCTTCACTTTGCAAGAACAACAGGGATAGAGGGAAGAATGAGGGGTCTAGGAAGTTTGATTGTTCGAGCTCGAAGAATTCCAAAATTGGATTCTCGAGGAGTTGGGAAGATCCGATTCTTCTCCGCCGCAACGACTTCTTCTCTATTCGGCGGTGAAGAAGCTGCTTCTTGCTCTGTGTCGTCGAGGACTTGCTCCGATTCTTACTTCTCCTGGAAATGGAACCACTTCGGAGGACAGAGGAGAACAATGTTCATCGAAACTCAATCAACGCCGAATCCTTCGTCTCTCATGTTCTATCCTGGAAAGCCTGTTATGGAAGTTGGGAGTGCTGATTTTCCAAATGCGCGTTCGGCTATGAACTCCCCACTAGCAAAAGCCCTTTATGGCGTTGACGGCGTTGTTCGAGTCTTCTTTGGATCAGATTTTGTTACTATAACAAAATCAAATGATGCTTCTTGGGATTTCCTAAAGCCCGAAATTTTTGCCGCAATCATGGATTTCTATTCTTCTGGGCAACCACTGTTTCTAGACTCCAAAACTGCTGCTGCCATGGACACAGCCATCAATGAAGATGATTCTGAAACCGTTGCTATGATCAAAGAGCTGCTTGAGACCCGTATCCGACCAGCAGTGCAAGATGATGGCGGAGATATTGAATACCGAGGATATGATGAAGAAACTGGTATTGTCAAATTGAGAATGCAAGGAGCATGCAGTGGCTGTCCAAGTTCATCTGTCACTTTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAGGATTATACGATCTTTGTAA

Protein sequence

MDPTEPKLKLCVSTTLYVRYLSRRTLHFARTTGIEGRMRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYFSWKWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVRIIRSL

Homology

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BLAST of Spg039596.1 vs. NCBI nr
Match: XP_022960817.1 (nifU-like protein 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-128
Identity = 239/253 (94.47%), Postives = 244/253 (96.44%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRIP L+SRGVGKIRF SAA TSSLF GEEAAS SVSSRT SDSY  SW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
           KWN FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGVD
Sbjct: 61  KWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVD 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GVVRVFFGSDFVT+TKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPEVR 290
           IENMLMHYVPEV+
Sbjct: 241 IENMLMHYVPEVK 253

[Close]

BLAST of Spg039596.1 vs. NCBI nr
Match: KAG7016340.1 (NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-128
Identity = 233/258 (90.31%), Postives = 247/258 (95.74%), Query Frame = 0


Query: 35  EGRMRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF 94
           EG+MRGLGSLIVRARRI  L S GVGKIRFFSAATTSS+FGG+EAASCSVSSRTCSD   
Sbjct: 10  EGKMRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLL 69

Query: 95  -SWKWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALY 154
            SW+W+ FGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALY
Sbjct: 70  RSWRWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALY 129

Query: 155 GVDGVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAI 214
           GVDGV+RVFFGSDFVT+TKSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAI
Sbjct: 130 GVDGVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAI 189

Query: 215 NEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTL 274
           NEDDSETVAMIKELLETRIRP VQDDGGDIEYRGY+EETGIVKLRMQGACSGCPSSSVTL
Sbjct: 190 NEDDSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTL 249

Query: 275 KSGIENMLMHYVPEVRII 292
           KSGIENMLMHYVPEVR++
Sbjct: 250 KSGIENMLMHYVPEVRVM 267

[Close]

BLAST of Spg039596.1 vs. NCBI nr
Match: KAG7032942.1 (NifU-like protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 468.0 bits (1203), Expect = 6.0e-128
Identity = 240/256 (93.75%), Postives = 246/256 (96.09%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRIP L+SRGVGKIRF SAA TSSLF GEEAAS SVSSRT SDSY  SW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 98  KWNH-FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGV 157
           KWN  FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGV
Sbjct: 61  KWNRLFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGV 120

Query: 158 DGVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINE 217
           DGVVRVFFGSDFVT+TKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINE
Sbjct: 121 DGVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINE 180

Query: 218 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 277
           DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS
Sbjct: 181 DDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKS 240

Query: 278 GIENMLMHYVPEVRII 292
           GIENMLMHYVPEVR++
Sbjct: 241 GIENMLMHYVPEVRVV 256

[Close]

BLAST of Spg039596.1 vs. NCBI nr
Match: XP_022990438.1 (nifU-like protein 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 467.6 bits (1202), Expect = 7.8e-128
Identity = 237/253 (93.68%), Postives = 242/253 (95.65%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRIP L+ RGVGKIRF SAA TSSLF GEEAAS SVSSRTCSDSY  SW
Sbjct: 1   MRGLGSLIVRARRIPGLNPRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTCSDSYLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
           KWN FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGVD
Sbjct: 61  KWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVD 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GVVRVFFGSDFVT+ KSNDASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVRKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIV LRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVNLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPEVR 290
           IENMLMHYVPEV+
Sbjct: 241 IENMLMHYVPEVK 253

[Close]

BLAST of Spg039596.1 vs. NCBI nr
Match: KAG6602261.1 (NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 467.2 bits (1201), Expect = 1.0e-127
Identity = 238/251 (94.82%), Postives = 242/251 (96.41%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRIP L+SRGVGKIRF SAA TSSLF GEEAAS SVSSRT SDSY  SW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
           KWN FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGVD
Sbjct: 61  KWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVD 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GVVRVFFGSDFVT+TKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPE 288
           IENMLMHYVPE
Sbjct: 241 IENMLMHYVPE 251

[Close]

BLAST of Spg039596.1 vs. ExPASy Swiss-Prot
Match: Q9LIG6 (NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 6.9e-95
Identity = 182/263 (69.20%), Postives = 210/263 (79.85%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEE----AASCSVSSRT----- 97
           M+G+  L+    RI     + V      +++++SSL         +A+  ++SRT     
Sbjct: 1   MKGIARLVTSLSRIG--GRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 98  --CSDSYFSWKWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNS 157
              + S  + KWN  GGQRRTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ S
Sbjct: 61  PSLNSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGS 120

Query: 158 PLAKALYGVDGVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTA 217
           PLAK++Y +DGVVRVFFGSDFVT+TKS+D SWD LKPEIFAA+MDFYSSGQPLFLDS+ A
Sbjct: 121 PLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAA 180

Query: 218 AAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGC 277
           AA DTAI+EDDSETVAMIKELLETRIRPAVQDDGGDIEY G+D E+GIVKLRMQGACSGC
Sbjct: 181 AAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGC 240

Query: 278 PSSSVTLKSGIENMLMHYVPEVR 290
           PSSSVTLKSGIENMLMHYV EV+
Sbjct: 241 PSSSVTLKSGIENMLMHYVSEVK 261

[Close]

BLAST of Spg039596.1 vs. ExPASy Swiss-Prot
Match: Q9C8J2 (NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 5.1e-90
Identity = 167/238 (70.17%), Postives = 192/238 (80.67%), Query Frame = 0


Query: 52  PKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYFSWKWNHFGGQRRTMFIE 111
           P   S  + +   F +A T       E+ + + S      S F + W     QRRTMFI+
Sbjct: 22  PSSSSLLISRRSLFISAATHFPVNFSESITTNASRNCSRSSSFPFNWIE---QRRTMFIQ 81

Query: 112 TQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVVRVFFGSDFVTIT 171
           TQSTPNPSSLMF PGKPVME+GSADFPN+RSAM+SPLAKA++ +DGVVRVF+GSDFVT+T
Sbjct: 82  TQSTPNPSSLMFSPGKPVMEIGSADFPNSRSAMSSPLAKAIFAIDGVVRVFYGSDFVTVT 141

Query: 172 KSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLETR 231
           KS+D +WD LKP+IFA +MDFYSSGQPLFLDS+  AA DTAI+EDDSETVAMIKELLETR
Sbjct: 142 KSDDVTWDILKPDIFAVVMDFYSSGQPLFLDSQATAAKDTAIHEDDSETVAMIKELLETR 201

Query: 232 IRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVR 290
           IRP+VQDDGGDIEY G+D ETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYV EV+
Sbjct: 202 IRPSVQDDGGDIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVK 256

[Close]

BLAST of Spg039596.1 vs. ExPASy Swiss-Prot
Match: Q8SY96 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=CG32500 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.4e-58
Identity = 114/191 (59.69%), Postives = 142/191 (74.35%), Query Frame = 0


Query: 105 RRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLAKALYGVDGVVRVFF 164
           RR+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A NSPLAK L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPESLKFLPGVDVLGKGNTYDFPNGTTAHNSPLAKLLFRVEGVKGVFF 123

Query: 165 GSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAM 224
           G+DFVTI+K   A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLNDAQPNA--DTEILEDDDETVMM 183

Query: 225 IKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 284
           IKELL+TRIRP VQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EGGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 285 YVPEVRIIRSL 295
           Y+PEV  +  +
Sbjct: 244 YIPEVESVEQV 250

[Close]

BLAST of Spg039596.1 vs. ExPASy Swiss-Prot
Match: B3NYF7 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila erecta OX=7220 GN=GG17526 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-58
Identity = 113/191 (59.16%), Postives = 142/191 (74.35%), Query Frame = 0


Query: 105 RRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLAKALYGVDGVVRVFF 164
           RR+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A +SPLAK L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPDSLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 165 GSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAM 224
           G+DFVTI+K   A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLHDAQPNA--DTEILEDDDETVMM 183

Query: 225 IKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 284
           IKELL+TRIRP VQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EAGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 285 YVPEVRIIRSL 295
           Y+PEV  +  +
Sbjct: 244 YIPEVESVEQV 250

[Close]

BLAST of Spg039596.1 vs. ExPASy Swiss-Prot
Match: B4PZ52 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX=7245 GN=GE15286 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-58
Identity = 113/191 (59.16%), Postives = 142/191 (74.35%), Query Frame = 0


Query: 105 RRTMFIETQSTPNPSSLMFYPGKPVMEVGSA-DFPNARSAMNSPLAKALYGVDGVVRVFF 164
           RR+MFI+TQ TPNP SL F PG  V+  G+  DFPN  +A +SPLAK L+ V+GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPDSLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 165 GSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAM 224
           G+DFVTI+K   A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD ETV M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLHDAQPNA--DTEILEDDDETVMM 183

Query: 225 IKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMH 284
           IKELL+TRIRP VQ+DGGDI + GY  E G+VKL+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EAGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 285 YVPEVRIIRSL 295
           Y+PEV  +  +
Sbjct: 244 YIPEVESVEQV 250

[Close]

BLAST of Spg039596.1 vs. ExPASy TrEMBL
Match: A0A6J1H8H2 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.0e-128
Identity = 239/253 (94.47%), Postives = 244/253 (96.44%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRIP L+SRGVGKIRF SAA TSSLF GEEAAS SVSSRT SDSY  SW
Sbjct: 1   MRGLGSLIVRARRIPGLNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
           KWN FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGVD
Sbjct: 61  KWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVD 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GVVRVFFGSDFVT+TKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVTKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPEVR 290
           IENMLMHYVPEV+
Sbjct: 241 IENMLMHYVPEVK 253

[Close]

BLAST of Spg039596.1 vs. ExPASy TrEMBL
Match: A0A6J1JTA1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.8e-128
Identity = 237/253 (93.68%), Postives = 242/253 (95.65%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRIP L+ RGVGKIRF SAA TSSLF GEEAAS SVSSRTCSDSY  SW
Sbjct: 1   MRGLGSLIVRARRIPGLNPRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTCSDSYLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
           KWN FGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLA+ALYGVD
Sbjct: 61  KWNRFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVD 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GVVRVFFGSDFVT+ KSNDASWDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVRKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIV LRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVNLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPEVR 290
           IENMLMHYVPEV+
Sbjct: 241 IENMLMHYVPEVK 253

[Close]

BLAST of Spg039596.1 vs. ExPASy TrEMBL
Match: A0A6J1BW08 (nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111006227 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 6.0e-126
Identity = 228/253 (90.12%), Postives = 241/253 (95.26%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVR RRIP++DSRG GKIR  S ATTSSLFGGE+AASCS +SR+CS+SY  SW
Sbjct: 1   MRGLGSLIVRVRRIPRMDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
           KWN FGGQRRTMFI+TQSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLAKALYG+D
Sbjct: 61  KWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGID 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GVVRVFFGSDFVT+TKS DA+WDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED
Sbjct: 121 GVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSE VAMIKELLETRIRPAVQDDGGDIEYRGY+EETGIV LRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSEIVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPEVR 290
           IENMLMHYVPEV+
Sbjct: 241 IENMLMHYVPEVK 253

[Close]

BLAST of Spg039596.1 vs. ExPASy TrEMBL
Match: A0A6J1FIB0 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668 PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 1.0e-125
Identity = 229/253 (90.51%), Postives = 240/253 (94.86%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRI  L S GVGKIRFFSAATTSS+FGG EAASCSVSSRTC D    SW
Sbjct: 1   MRGLGSLIVRARRISPLGSHGVGKIRFFSAATTSSIFGGNEAASCSVSSRTCFDPLLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
           +W+ FGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD
Sbjct: 61  RWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GV+RVFFGSDFVT+TKSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSETVAMIKELLETRIRP VQDDGGDIEYRGY+EETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPEVR 290
           IENMLMHYVPEV+
Sbjct: 241 IENMLMHYVPEVK 253

[Close]

BLAST of Spg039596.1 vs. ExPASy TrEMBL
Match: A0A6J1JQB1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.3e-125
Identity = 228/253 (90.12%), Postives = 241/253 (95.26%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYF-SW 97
           MRGLGSLIVRARRI  L S G GKIRFFSAATTSS+FGG+EAASCSVSSRTCSD +  SW
Sbjct: 1   MRGLGSLIVRARRISTLGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPFLRSW 60

Query: 98  KWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVD 157
            W+ FGGQ+RTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNAR+AMNSPLAKALYGVD
Sbjct: 61  SWSQFGGQKRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYGVD 120

Query: 158 GVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINED 217
           GV+RVFFGSDFVT+TKSN+ASWDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINED
Sbjct: 121 GVIRVFFGSDFVTVTKSNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINED 180

Query: 218 DSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSG 277
           DSETVAMIKELLETRIRP VQDDGGDIEYRGY+EETGIVKLRMQGACSGCPSSSVTLKSG
Sbjct: 181 DSETVAMIKELLETRIRPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSG 240

Query: 278 IENMLMHYVPEVR 290
           IENMLMHYVPEV+
Sbjct: 241 IENMLMHYVPEVK 253

[Close]

BLAST of Spg039596.1 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 348.6 bits (893), Expect = 4.9e-96
Identity = 182/263 (69.20%), Postives = 210/263 (79.85%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEE----AASCSVSSRT----- 97
           M+G+  L+    RI     + V      +++++SSL         +A+  ++SRT     
Sbjct: 1   MKGIARLVTSLSRIG--GRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 98  --CSDSYFSWKWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNS 157
              + S  + KWN  GGQRRTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ S
Sbjct: 61  PSLNSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGS 120

Query: 158 PLAKALYGVDGVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTA 217
           PLAK++Y +DGVVRVFFGSDFVT+TKS+D SWD LKPEIFAA+MDFYSSGQPLFLDS+ A
Sbjct: 121 PLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAA 180

Query: 218 AAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGC 277
           AA DTAI+EDDSETVAMIKELLETRIRPAVQDDGGDIEY G+D E+GIVKLRMQGACSGC
Sbjct: 181 AAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGC 240

Query: 278 PSSSVTLKSGIENMLMHYVPEVR 290
           PSSSVTLKSGIENMLMHYV EV+
Sbjct: 241 PSSSVTLKSGIENMLMHYVSEVK 261

[Close]

BLAST of Spg039596.1 vs. TAIR 10
Match: AT1G51390.1 (NFU domain protein 5 )

HSP 1 Score: 332.4 bits (851), Expect = 3.7e-91
Identity = 167/238 (70.17%), Postives = 192/238 (80.67%), Query Frame = 0


Query: 52  PKLDSRGVGKIRFFSAATTSSLFGGEEAASCSVSSRTCSDSYFSWKWNHFGGQRRTMFIE 111
           P   S  + +   F +A T       E+ + + S      S F + W     QRRTMFI+
Sbjct: 22  PSSSSLLISRRSLFISAATHFPVNFSESITTNASRNCSRSSSFPFNWIE---QRRTMFIQ 81

Query: 112 TQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVVRVFFGSDFVTIT 171
           TQSTPNPSSLMF PGKPVME+GSADFPN+RSAM+SPLAKA++ +DGVVRVF+GSDFVT+T
Sbjct: 82  TQSTPNPSSLMFSPGKPVMEIGSADFPNSRSAMSSPLAKAIFAIDGVVRVFYGSDFVTVT 141

Query: 172 KSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLETR 231
           KS+D +WD LKP+IFA +MDFYSSGQPLFLDS+  AA DTAI+EDDSETVAMIKELLETR
Sbjct: 142 KSDDVTWDILKPDIFAVVMDFYSSGQPLFLDSQATAAKDTAIHEDDSETVAMIKELLETR 201

Query: 232 IRPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVR 290
           IRP+VQDDGGDIEY G+D ETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYV EV+
Sbjct: 202 IRPSVQDDGGDIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVK 256

[Close]

BLAST of Spg039596.1 vs. TAIR 10
Match: AT3G20970.2 (NFU domain protein 4 )

HSP 1 Score: 275.8 bits (704), Expect = 4.1e-74
Identity = 146/223 (65.47%), Postives = 172/223 (77.13%), Query Frame = 0


Query: 38  MRGLGSLIVRARRIPKLDSRGVGKIRFFSAATTSSLFGGEE----AASCSVSSRT----- 97
           M+G+  L+    RI     + V      +++++SSL         +A+  ++SRT     
Sbjct: 1   MKGIARLVTSLSRIG--GRKVVSGTSTVTSSSSSSLLLSRRSLFISATNLLNSRTKDSAL 60

Query: 98  --CSDSYFSWKWNHFGGQRRTMFIETQSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNS 157
              + S  + KWN  GGQRRTMFI+TQSTPNPSSLMFYPGKPVMEVGSADFPN RSA+ S
Sbjct: 61  PSLNSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGS 120

Query: 158 PLAKALYGVDGVVRVFFGSDFVTITKSNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTA 217
           PLAK++Y +DGVVRVFFGSDFVT+TKS+D SWD LKPEIFAA+MDFYSSGQPLFLDS+ A
Sbjct: 121 PLAKSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAA 180

Query: 218 AAMDTAINEDDSETVAMIKELLETRIRPAVQDDGGDIEYRGYD 250
           AA DTAI+EDDSETVAMIKELLETRIRPAVQDDGGDIEY G+D
Sbjct: 181 AAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFD 221

[Close]

BLAST of Spg039596.1 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 63.9 bits (154), Expect = 2.4e-10
Identity = 37/105 (35.24%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 193 YSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLET---RIRPAVQDDGGDIEYRGYD 252
           + S + LF  SK   A+ T    D    V + +E +E+    IRP +  DGG++    ++
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 253 EETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVRIIRSL 295
            +  IV++++QGAC  CPSS++T+K GIE  LM  +PE+  + +L
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEAL 157

[Close]

BLAST of Spg039596.1 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 63.9 bits (154), Expect = 2.4e-10
Identity = 37/105 (35.24%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 193 YSSGQPLFLDSKTAAAMDTAINEDDSETVAMIKELLET---RIRPAVQDDGGDIEYRGYD 252
           + S + LF  SK   A+ T    D    V + +E +E+    IRP +  DGG++    ++
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 253 EETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVRIIRSL 295
            +  IV++++QGAC  CPSS++T+K GIE  LM  +PE+  + +L
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEAL 157

The following BLAST results are available for this feature:

BLAST of Spg039596.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960817.1	2.1e-128	94.47	nifU-like protein 4, mitochondrial [Cucurbita moschata]	[more]
KAG7016340.1	2.1e-128	90.31	NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG7032942.1	6.0e-128	93.75	NifU-like protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022990438.1	7.8e-128	93.68	nifU-like protein 4, mitochondrial [Cucurbita maxima]	[more]
KAG6602261.1	1.0e-127	94.82	NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Spg039596.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIG6	6.9e-95	69.20	NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2...	[more]
Q9C8J2	5.1e-90	70.17	NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2...	[more]
Q8SY96	1.4e-58	59.69	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogas...	[more]
B3NYF7	3.0e-58	59.16	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila erecta OX...	[more]
B4PZ52	3.0e-58	59.16	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX...	[more]

BLAST of Spg039596.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8H2	1.0e-128	94.47	nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505...	[more]
A0A6J1JTA1	3.8e-128	93.68	nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 P...	[more]
A0A6J1BW08	6.0e-126	90.12	nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11100622...	[more]
A0A6J1FIB0	1.0e-125	90.51	nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668...	[more]
A0A6J1JQB1	1.3e-125	90.12	nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 P...	[more]

BLAST of Spg039596.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20970.1	4.9e-96	69.20	NFU domain protein 4	[more]
AT1G51390.1	3.7e-91	70.17	NFU domain protein 5	[more]
AT3G20970.2	4.1e-74	65.47	NFU domain protein 4	[more]
AT5G49940.1	2.4e-10	35.24	NIFU-like protein 2	[more]
AT5G49940.2	2.4e-10	35.24	NIFU-like protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014824	Scaffold protein Nfu/NifU, N-terminal	SMART	SM00932	Nfu_N_3a	coord: 110..196 e-value: 3.8E-44 score: 162.7
IPR014824	Scaffold protein Nfu/NifU, N-terminal	PFAM	PF08712	Nfu_N	coord: 110..196 e-value: 6.7E-29 score: 99.8
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 224..289 e-value: 1.8E-28 score: 98.5
IPR036498	Scaffold protein Nfu/NifU, N-terminal domain superfamily	GENE3D	3.30.1370.70		coord: 99..202 e-value: 7.0E-36 score: 124.2
IPR036498	Scaffold protein Nfu/NifU, N-terminal domain superfamily	SUPERFAMILY	110836	Hypothetical protein SAV1430	coord: 108..198
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 218..293 e-value: 1.3E-32 score: 113.6
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 210..292
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 64..290
None	No IPR available	PANTHER	PTHR11178:SF43	NIFU-LIKE PROTEIN 5, MITOCHONDRIAL	coord: 64..290

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg039596	Spg039596	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg039596.1-three_prime_utr	Spg039596.1-three_prime_utr-scaffold10:41139083..41140217	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg039596.1-cds	Spg039596.1-cds-scaffold10:41140218..41140353	CDS
Spg039596.1-cds	Spg039596.1-cds-scaffold10:41140463..41140566	CDS
Spg039596.1-cds	Spg039596.1-cds-scaffold10:41141589..41141764	CDS
Spg039596.1-cds	Spg039596.1-cds-scaffold10:41142874..41143035	CDS
Spg039596.1-cds	Spg039596.1-cds-scaffold10:41143121..41143427	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg039596.1	Spg039596.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding