Spg033627.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg033627.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	isoflavone reductase-like protein
Location	scaffold13: 38766350 .. 38768820 (+)
Sequence length	927
RNA-Seq Expression	Spg033627.1
Synteny	Spg033627.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGAATTGCATTTTCCCGCCGTAGGCGTAATTTTGAAGCGAAGGAGACTGTCTTCATCAATGGCGGAGAAAAGCAAAGTTCTGATCATCGGAGGCACTGGTTATATCGGAAAGTTCGTGGTTGAAGCGAGCGCCAAGGCCGGCCATCCTACTTTCGCTCTGATCAGAGAGAGCACCGTCGCCGATCCTGTAAAGGCAAAGCTCGTCGAGAATTTCAAGAATTTAGGAGTCAAATTGATCACTGTAAGTTCAAATTGATCTCTCTTTCTCGATCTCCGTGATTGATCTATATGGAGAAATTCAATTCGTTCTGGATTGTGAATGTGTGATTAAGTTTGTTTAACGGTGGAGATTCGATGTTGCAGGGAGATCTTTATGATCATGAAAGTCTGGTGAAGGCGATCAAGCAAGTGGATGTGGTGATTTCGACGGTTGGACAAATGCAATTGGCGGATCAGGTCAAGATCATTGCTGCAATCAAAGAGGCCGGCAATGTCAAGGTACCGATGAATTCAGTCTTTTGCTTTCTGAACTTCAATTGGCTTCTTAAATTGAATCTGACCTCGTTTTATGATTCAAATTCTGTGAAATGAAATTATCCGAGTTTAAATGTAGCTTGCGAATTTAGATCAAATTGTATAAAACATTGTTTTATAATTATTCCAAGAAGGAAAAATGTGTATATATAATAATATTCTATAACTCTGTAAAATGGTAGATGGGGAGAAGATTTAGGACTTTATGTGTTGAGATTGCATGGAAAAAATTTGTGGCAACCTCTTAATAACTATAGGATGTGAATTCGAGAATTTACGAACTTCTTGATAACCATTGGATATAGATTTCTACAGTTTTTCTCTTATACATCTAATAATATAAAACTATCAATAATTTCTTCCGTACGTATGGGACAATTATCCAAATTATTTAAGTTTGTGTCGGTATTTTGACGATTTTCTTATTGAAATAAAAACATTAAACTTCTGTTGGTAATTTAATAATTTTAGTACTGGGAAAATTTATGATTATGAAGTGGGCCGCCCTAACTGATCTACAGGCCCATGGGCCATTATACCATTTTATTATCAAAATTGCCCCTTTATTAGAGTAAAATATTGTAAAATGTTATTTCAAATTGGTTATATTTGAAATAATTCACGAGTTATATTTACTGTTAGAATTATGTTTACGTTAGTGAAATGAAAGAAATTTAACTTTATAACAAAAGAACGTTTTGTTTTCAACACCGTAGAGATTTTTCCCTTCGGAGTTTGGTGTGGATGTGGACAGACTCCACGCAGTTGAGCCAGCAAAATCTGCATTGGCCGTAAAGGCTCAAATCCGACGGGCAGTTGAGAAGGAAGGGATACCCTACACTTATGTGTCCTCCAACTGTTTCAGTGGCTATTTTCTCCCCTCACTTATGCAGCCGGGACTCACCTCCCCTCCTAGAGACAAAGTCGTCATCCCCGGTGATGGACAGCCCAAAGGTTAGTTGTCCCAAAATTTCGATTGCTCAAGATTTGAACCACATACCCTCTTGATCAATAAATATAGTCCTATGTTAATTGAAAATCTAGAAAATTCTTAGATATACAATTATGTAAGCACAATATCCTAAGTGGGTCGAATTCTAAACTAGATAATCATCTACTAGTGCTGAAATAACTGCATTTTTGTCAAAGTTTTGCAACACTCACTTTGTTTTTAGAATAATTTTCAAAAAGTATATTAGACAATTTAATGGTTTTGTTTGATAAAATGAACACACAGCAATTTTTAACTTCGAGGAAGACATTGGCACTTACACTATCAAAGCTGTGGACGACCCAAGAACTGAAAACAAAATCCTCTACATCAAGCCGCCCAAAAACACCTACTCTTTCAACGACCTCGTAGCTCTGTGGGAGCAAAAGATTGGCAAAGCCTTGGAAAAGGTCTACGTTCCAGAGGAGCAGATCCTCAAGGACATCCAAGGTCAGTCTCTTTTACGTACGAATGTGAATAAATAATTAAATTTACCGTAACTTATCAACTTAAATCTCATATACGTGTGAGATTCTAAGAAAGTTTTATAAACTTGACAGAGTCTCCACTTCCGATCAATGTGATTTTGGCCCTCAACCACTCGATGTTTGTGAAGGGAGATCAGACCAACTTTGAGATCGAGCCATCCTTTGGAGTCGAGGCTTCGGCTCTCTACCCCGACGTCAAATACACCACGGTCGATGAGTCGCTCTCTCGATTCGTTTGAAGGAAATTAAAAGAAAAGAAAACTTGGGTGGTGCCTTTGTTTCTGATTTGTAGTAAATAATTCTGCATAGCTTTTTCTTGCTGCAGATCGAGTGTTCTGTTTTATGGTCTACTCTTTTAGTTTCTGCCTATTGTCGTTCTATATGTTATGTGCCCACAGAGTGTGTGTTTTATAATGAATATAGAGCTTTCTTCGAGAAATATGTGTTTGATAAGTAT

mRNA sequence

ATGGCGGAGAAAAGCAAAGTTCTGATCATCGGAGGCACTGGTTATATCGGAAAGTTCGTGGTTGAAGCGAGCGCCAAGGCCGGCCATCCTACTTTCGCTCTGATCAGAGAGAGCACCGTCGCCGATCCTGTAAAGGCAAAGCTCGTCGAGAATTTCAAGAATTTAGGAGTCAAATTGATCACTGGAGATCTTTATGATCATGAAAGTCTGGTGAAGGCGATCAAGCAAGTGGATGTGGTGATTTCGACGGTTGGACAAATGCAATTGGCGGATCAGGTCAAGATCATTGCTGCAATCAAAGAGGCCGGCAATGTCAAGAGATTTTTCCCTTCGGAGTTTGGTGTGGATGTGGACAGACTCCACGCAGTTGAGCCAGCAAAATCTGCATTGGCCGTAAAGGCTCAAATCCGACGGGCAGTTGAGAAGGAAGGGATACCCTACACTTATGTGTCCTCCAACTGTTTCAGTGGCTATTTTCTCCCCTCACTTATGCAGCCGGGACTCACCTCCCCTCCTAGAGACAAAGTCGTCATCCCCGGTGATGGACAGCCCAAAGCAATTTTTAACTTCGAGGAAGACATTGGCACTTACACTATCAAAGCTGTGGACGACCCAAGAACTGAAAACAAAATCCTCTACATCAAGCCGCCCAAAAACACCTACTCTTTCAACGACCTCGTAGCTCTGTGGGAGCAAAAGATTGGCAAAGCCTTGGAAAAGGTCTACGTTCCAGAGGAGCAGATCCTCAAGGACATCCAAGAGTCTCCACTTCCGATCAATGTGATTTTGGCCCTCAACCACTCGATGTTTGTGAAGGGAGATCAGACCAACTTTGAGATCGAGCCATCCTTTGGAGTCGAGGCTTCGGCTCTCTACCCCGACGTCAAATACACCACGGTCGATGAGTCGCTCTCTCGATTCGTTTGA

Coding sequence (CDS)

ATGGCGGAGAAAAGCAAAGTTCTGATCATCGGAGGCACTGGTTATATCGGAAAGTTCGTGGTTGAAGCGAGCGCCAAGGCCGGCCATCCTACTTTCGCTCTGATCAGAGAGAGCACCGTCGCCGATCCTGTAAAGGCAAAGCTCGTCGAGAATTTCAAGAATTTAGGAGTCAAATTGATCACTGGAGATCTTTATGATCATGAAAGTCTGGTGAAGGCGATCAAGCAAGTGGATGTGGTGATTTCGACGGTTGGACAAATGCAATTGGCGGATCAGGTCAAGATCATTGCTGCAATCAAAGAGGCCGGCAATGTCAAGAGATTTTTCCCTTCGGAGTTTGGTGTGGATGTGGACAGACTCCACGCAGTTGAGCCAGCAAAATCTGCATTGGCCGTAAAGGCTCAAATCCGACGGGCAGTTGAGAAGGAAGGGATACCCTACACTTATGTGTCCTCCAACTGTTTCAGTGGCTATTTTCTCCCCTCACTTATGCAGCCGGGACTCACCTCCCCTCCTAGAGACAAAGTCGTCATCCCCGGTGATGGACAGCCCAAAGCAATTTTTAACTTCGAGGAAGACATTGGCACTTACACTATCAAAGCTGTGGACGACCCAAGAACTGAAAACAAAATCCTCTACATCAAGCCGCCCAAAAACACCTACTCTTTCAACGACCTCGTAGCTCTGTGGGAGCAAAAGATTGGCAAAGCCTTGGAAAAGGTCTACGTTCCAGAGGAGCAGATCCTCAAGGACATCCAAGAGTCTCCACTTCCGATCAATGTGATTTTGGCCCTCAACCACTCGATGTTTGTGAAGGGAGATCAGACCAACTTTGAGATCGAGCCATCCTTTGGAGTCGAGGCTTCGGCTCTCTACCCCGACGTCAAATACACCACGGTCGATGAGTCGCTCTCTCGATTCGTTTGA

Protein sequence

MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLHAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPGDGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEKVYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTVDESLSRFV

Homology

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BLAST of Spg033627.1 vs. NCBI nr
Match: XP_023002443.1 (isoflavone reductase-like protein [Cucurbita maxima])

HSP 1 Score: 566.6 bits (1459), Expect = 1.3e-157
Identity = 278/308 (90.26%), Postives = 299/308 (97.08%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFAL+RESTVADPVKAKLVENFKNLGVKLI
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALVRESTVADPVKAKLVENFKNLGVKLI 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIK+VDVVIST+GQMQLADQ+KIIAAIKEAGNVKRFFPSEFG+DVDRL
Sbjct: 61  TGDLYDHESLVKAIKEVDVVISTLGQMQLADQIKIIAAIKEAGNVKRFFPSEFGMDVDRL 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKS LAVKAQIRRA+E+EGIPYTYVSSN F+GYFLP+L QPGLT+PPRDKVVIPG
Sbjct: 121 HAVEPAKSVLAVKAQIRRAIEREGIPYTYVSSNYFNGYFLPTLSQPGLTAPPRDKVVIPG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG PKA+FN EEDIGTYTIKAVDDP+TENKILYI+PPKNTYSFNDLVALWE+KI K LEK
Sbjct: 181 DGHPKAVFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPE+QILKDIQ++PLP+NVILALNHS FVKGDQTNFEIEPSFGVEASALYP+V+YTTV
Sbjct: 241 IYVPEDQILKDIQDAPLPLNVILALNHSAFVKGDQTNFEIEPSFGVEASALYPEVRYTTV 300

Query: 301 DESLSRFV 309
           DE LSRFV
Sbjct: 301 DEYLSRFV 308

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BLAST of Spg033627.1 vs. NCBI nr
Match: KAG7020505.1 (hypothetical protein SDJN02_17190 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.7 bits (1454), Expect = 4.9e-157
Identity = 280/308 (90.91%), Postives = 298/308 (96.75%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAKAGH TFALIRESTVADPVKAKLVENFKNLGVKLI
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHTTFALIRESTVADPVKAKLVENFKNLGVKLI 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIK+VDVVIST+GQMQLADQVKIIAAIKEAGNVKRFFPSEFG+DVDRL
Sbjct: 61  TGDLYDHESLVKAIKEVDVVISTLGQMQLADQVKIIAAIKEAGNVKRFFPSEFGMDVDRL 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKSALAVKAQIRRA+E+EGIPYTYVSSNCF+GYFLP+L QPGLT+PPRDKVVI G
Sbjct: 121 HAVEPAKSALAVKAQIRRAIEREGIPYTYVSSNCFNGYFLPTLSQPGLTAPPRDKVVISG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG PK IFN EEDIGTYTIKAVDDP+TENKILYI+PPKNTYSFNDLVALWE+KI K LEK
Sbjct: 181 DGHPKVIFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPE+QILKDIQE+PLP+NVILALNHS+FVKGDQTNFEIE SFGVEASALYP+V+YTTV
Sbjct: 241 IYVPEDQILKDIQEAPLPLNVILALNHSVFVKGDQTNFEIEASFGVEASALYPEVRYTTV 300

Query: 301 DESLSRFV 309
           DE LSRFV
Sbjct: 301 DEYLSRFV 308

[Close]

BLAST of Spg033627.1 vs. NCBI nr
Match: XP_022951674.1 (isoflavone reductase-like protein [Cucurbita moschata])

HSP 1 Score: 563.9 bits (1452), Expect = 8.4e-157
Identity = 279/308 (90.58%), Postives = 298/308 (96.75%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIK+VDVVIST+GQMQLADQVKIIAAIKEA NVKRFFPSEFG+DVDRL
Sbjct: 61  TGDLYDHESLVKAIKEVDVVISTLGQMQLADQVKIIAAIKEACNVKRFFPSEFGMDVDRL 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKSALA+KAQIRRA+E+EGIPYTYVSSNCF+GYFLP+L QPGLT+PPRDKVVI G
Sbjct: 121 HAVEPAKSALAIKAQIRRAIEREGIPYTYVSSNCFNGYFLPTLSQPGLTAPPRDKVVISG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG PK IFN EEDIGTYTIKAVDDP+TENKILYI+PPKNTYSFNDLVALWE+KI K LEK
Sbjct: 181 DGHPKVIFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPE+QILKDIQE+PLP+NVILALNHS+FVKGDQTNFEIE SFGVEASALYP+V+YTTV
Sbjct: 241 IYVPEDQILKDIQEAPLPLNVILALNHSVFVKGDQTNFEIEASFGVEASALYPEVRYTTV 300

Query: 301 DESLSRFV 309
           DE LSRFV
Sbjct: 301 DEYLSRFV 308

[Close]

BLAST of Spg033627.1 vs. NCBI nr
Match: XP_023536776.1 (isoflavone reductase homolog TP7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.5 bits (1451), Expect = 1.1e-156
Identity = 278/308 (90.26%), Postives = 298/308 (96.75%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIK+VDVVIST+GQMQLADQ+KI+AAIKEAGNVKRFFPSEFG+DVDRL
Sbjct: 61  TGDLYDHESLVKAIKEVDVVISTLGQMQLADQIKIVAAIKEAGNVKRFFPSEFGMDVDRL 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKS LAVKAQIRRA+E+EGIPYTYVSSN F+GYFLP+L QPGLT+PPRDKVVIPG
Sbjct: 121 HAVEPAKSVLAVKAQIRRAIEREGIPYTYVSSNYFNGYFLPNLSQPGLTAPPRDKVVIPG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG PK IFN EEDIGTYTIKAVDDP+TENKILYI+PPKNTYSFNDLVALWE+KI K LEK
Sbjct: 181 DGLPKVIFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPE+QILKDIQE+PLP+NVILALNHS+FVKGDQTNFEIE SFGVEASALYP+V+YTTV
Sbjct: 241 IYVPEDQILKDIQEAPLPLNVILALNHSVFVKGDQTNFEIEASFGVEASALYPEVRYTTV 300

Query: 301 DESLSRFV 309
           DE LSRFV
Sbjct: 301 DEYLSRFV 308

[Close]

BLAST of Spg033627.1 vs. NCBI nr
Match: XP_038885595.1 (eugenol synthase 2-like [Benincasa hispida])

HSP 1 Score: 553.5 bits (1425), Expect = 1.1e-153
Identity = 272/305 (89.18%), Postives = 292/305 (95.74%), Query Frame = 0


Query: 4   KSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITGD 63
           KS+VLIIGGTGYIGKFVVEASAKAGHPTF L+REST+ADPVKAKLVENFKNLGVKLITGD
Sbjct: 7   KSRVLIIGGTGYIGKFVVEASAKAGHPTFVLVRESTIADPVKAKLVENFKNLGVKLITGD 66

Query: 64  LYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLHAV 123
           LYD ESLVKAIK+VDVVISTVGQMQLADQ KII AIKEAGNVKRFFPSEFG+DVDRL+AV
Sbjct: 67  LYDRESLVKAIKEVDVVISTVGQMQLADQSKIIDAIKEAGNVKRFFPSEFGLDVDRLNAV 126

Query: 124 EPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPGDGQ 183
           EPAKSALA+KA+IRRA+E+EGIPYTYVSSNCF+GYFLP+LMQPGLTSPP DKV+IPGDG 
Sbjct: 127 EPAKSALAIKAEIRRAIEREGIPYTYVSSNCFNGYFLPTLMQPGLTSPPTDKVIIPGDGH 186

Query: 184 PKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEKVYV 243
           PKAIFN EEDIG+YTIKAVDDPRTENKILYI PP NTYSFNDLVALWE+KIGK LEK+YV
Sbjct: 187 PKAIFNLEEDIGSYTIKAVDDPRTENKILYINPPNNTYSFNDLVALWEKKIGKPLEKLYV 246

Query: 244 PEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTVDES 303
           PE QIL+DIQE+PLPINVILALNHS+FVKGD+TNFEIEPSFGVEAS LYPDVKYTTVDE 
Sbjct: 247 PEHQILRDIQEAPLPINVILALNHSIFVKGDETNFEIEPSFGVEASTLYPDVKYTTVDEY 306

Query: 304 LSRFV 309
           LSRFV
Sbjct: 307 LSRFV 311

[Close]

BLAST of Spg033627.1 vs. ExPASy Swiss-Prot
Match: B6VRE8 (Phenylcoumaran benzylic ether reductase TP7 OS=Nicotiana tabacum OX=4097 GN=TP7 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 7.7e-145
Identity = 247/308 (80.19%), Postives = 281/308 (91.23%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAK+GHPTFAL+REST++DPVK+K+VENFKNLGV ++
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKSGHPTFALVRESTLSDPVKSKIVENFKNLGVTIL 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
            GDLYDHESLVKAIKQVDVVIST+G MQL DQVK+IAAIKEAGN+KRFFPSEFG+DVD+ 
Sbjct: 61  HGDLYDHESLVKAIKQVDVVISTMGMMQLGDQVKLIAAIKEAGNIKRFFPSEFGMDVDKT 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           +AVEPAKSA AVK QIRRA+E EGIPYTYVS NCF+GYFLP+++QPG T PPRDKV+IPG
Sbjct: 121 NAVEPAKSAFAVKVQIRRAIEAEGIPYTYVSCNCFAGYFLPTMVQPGATVPPRDKVIIPG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG  KA+FN E DIGTYTIKAVDDPRT NK LYIKPPKNT SFN+LVA+WE+ IGK LEK
Sbjct: 181 DGNVKAVFNEEHDIGTYTIKAVDDPRTLNKTLYIKPPKNTLSFNELVAMWEKMIGKTLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +Y+PEEQILKDI+ SP+P+ VILA+NH+ FVKGDQTNF+IEPSFGVEAS LYPDVKYTTV
Sbjct: 241 IYIPEEQILKDIETSPMPLPVILAINHATFVKGDQTNFKIEPSFGVEASELYPDVKYTTV 300

Query: 301 DESLSRFV 309
           ++ L  FV
Sbjct: 301 EDYLGHFV 308

[Close]

BLAST of Spg033627.1 vs. ExPASy Swiss-Prot
Match: B2WSN1 (Eugenol synthase 1 OS=Petunia hybrida OX=4102 GN=EGS1 PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 1.7e-144
Identity = 248/307 (80.78%), Postives = 276/307 (89.90%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSK+LIIGGTGYIGKFVVEASAKAGHPTF L+RESTV+DP K K+VE+F N GV ++
Sbjct: 1   MAEKSKILIIGGTGYIGKFVVEASAKAGHPTFVLVRESTVSDPAKGKIVESFNNSGVTIL 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
            GDLYDHESLVKAIKQVDVVISTVGQMQLADQ KIIAAIKEAGN+KRFFPSEFG+DVD++
Sbjct: 61  YGDLYDHESLVKAIKQVDVVISTVGQMQLADQTKIIAAIKEAGNIKRFFPSEFGMDVDKV 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           +AVEPAKS  A+K QIRRA+E EGIPYTYVSSNCF+GYFLP+L+QPG T PPRDKV+I G
Sbjct: 121 NAVEPAKSTFAIKVQIRRAIEAEGIPYTYVSSNCFAGYFLPTLVQPGATDPPRDKVIISG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG  KA+FN E DIGTYTIKAVDDPRT NK LYIKPPKNT SFN+LVA+WE+ IGK LEK
Sbjct: 181 DGNAKAVFNEEHDIGTYTIKAVDDPRTLNKTLYIKPPKNTLSFNELVAIWEKLIGKTLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +Y+PEEQILKDI  SP+PIN+ILA+NHS FVKGDQTNF IEPSFGVEAS LYPDVKYTTV
Sbjct: 241 IYIPEEQILKDIATSPIPINIILAINHSTFVKGDQTNFVIEPSFGVEASELYPDVKYTTV 300

Query: 301 DESLSRF 308
           +E LS F
Sbjct: 301 EEYLSHF 307

[Close]

BLAST of Spg033627.1 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.1e-143
Identity = 249/309 (80.58%), Postives = 279/309 (90.29%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           M  KSK+LIIGGTGYIGKF+VEAS K GHPTFAL+RE+TV+DPVK KLVE F+NLGV L+
Sbjct: 1   MGSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLL 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
            GDLYDH+SLVKAIKQVDVVISTVG MQ+ADQ KIIAAIKEAGNVKRFFPSEFG DVD +
Sbjct: 61  YGDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHV 120

Query: 121 HAVEPAKS-ALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIP 180
           +AVEPAKS A AVKA IRRAVE EGIPYTYV+SNCF+GYFLP+L+QPG T+PPRDKV+IP
Sbjct: 121 NAVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIP 180

Query: 181 GDGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALE 240
           GDG PKAIFN EEDIGTYTIKAVDDPRT NKILY++P  N YSFN+LVALWE+KIGK LE
Sbjct: 181 GDGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLE 240

Query: 241 KVYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTT 300
           K+YVPEEQILKDIQE+P+PIN+ L +NHS+FVKGD TNFEIEPSFGVEAS LYP+VKYTT
Sbjct: 241 KIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTT 300

Query: 301 VDESLSRFV 309
           V+E L +FV
Sbjct: 301 VEEYLDQFV 309

[Close]

BLAST of Spg033627.1 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 2.5e-143
Identity = 249/308 (80.84%), Postives = 279/308 (90.58%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MA KSK+LIIGGTGYIGKF+VEASAK+GHPTFAL+RESTV+DPVK KLVE FK LGV L+
Sbjct: 1   MAHKSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLL 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
            GDLYDHESLVKA KQVDVVISTVG +QLADQVKIIAAIKEAGN+KRFFPSEFG DVDR+
Sbjct: 61  HGDLYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRV 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAK+A A KA+IRR  E EGIPYTYVSSN F+GYFLP+L QPGLTSPPR+KVVI G
Sbjct: 121 HAVEPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG  +A+FN E+DIGTYTI+AVDDPRT NKI+YIKP KN YSFN++VALWE+KIGK LEK
Sbjct: 181 DGNARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPEE++LKDIQESP+PINVILA+NHS+FVKGD TNFEIE SFGVEAS LYPDVKYTTV
Sbjct: 241 IYVPEEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTV 300

Query: 301 DESLSRFV 309
           +E L +FV
Sbjct: 301 EEYLQQFV 308

[Close]

BLAST of Spg033627.1 vs. ExPASy Swiss-Prot
Match: E1U332 (Isoflavone reductase-like protein OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 8.8e-141
Identity = 240/308 (77.92%), Postives = 278/308 (90.26%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MA+K+K+LIIGGTGYIGKF+VEASAK+ HPTFAL REST++DPVK K+++ FKN GV ++
Sbjct: 1   MADKTKILIIGGTGYIGKFIVEASAKSEHPTFALARESTISDPVKGKIIQGFKNSGVTIL 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIKQVDVVISTVGQ+QLADQVKIIAAIKEAGNVKRFFPS+FG DVDR 
Sbjct: 61  TGDLYDHESLVKAIKQVDVVISTVGQLQLADQVKIIAAIKEAGNVKRFFPSDFGTDVDRC 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKS+  +K+QIRRA+E EGIPYT+VS+N F+GY LP+L+QP +T+PPRDKV+I G
Sbjct: 121 HAVEPAKSSFEIKSQIRRAIEAEGIPYTFVSANYFAGYSLPTLVQPEVTAPPRDKVIILG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG  KA+FN E DIGTYTIKAVDD RT NKILYIKPPKN YSFN+LVALWE+KIGK LEK
Sbjct: 181 DGNAKAVFNEENDIGTYTIKAVDDARTLNKILYIKPPKNIYSFNELVALWEKKIGKTLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPEEQ+LK IQESP PIN+++A+NHS FVKGD TNF+IEPSFGVEAS LYPDVKYTTV
Sbjct: 241 IYVPEEQVLKQIQESPFPINIVMAINHSAFVKGDLTNFKIEPSFGVEASELYPDVKYTTV 300

Query: 301 DESLSRFV 309
           +E L +FV
Sbjct: 301 EEYLDQFV 308

[Close]

BLAST of Spg033627.1 vs. ExPASy TrEMBL
Match: A0A6J1KTK3 (isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111496281 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.3e-158
Identity = 278/308 (90.26%), Postives = 299/308 (97.08%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFAL+RESTVADPVKAKLVENFKNLGVKLI
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALVRESTVADPVKAKLVENFKNLGVKLI 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIK+VDVVIST+GQMQLADQ+KIIAAIKEAGNVKRFFPSEFG+DVDRL
Sbjct: 61  TGDLYDHESLVKAIKEVDVVISTLGQMQLADQIKIIAAIKEAGNVKRFFPSEFGMDVDRL 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKS LAVKAQIRRA+E+EGIPYTYVSSN F+GYFLP+L QPGLT+PPRDKVVIPG
Sbjct: 121 HAVEPAKSVLAVKAQIRRAIEREGIPYTYVSSNYFNGYFLPTLSQPGLTAPPRDKVVIPG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG PKA+FN EEDIGTYTIKAVDDP+TENKILYI+PPKNTYSFNDLVALWE+KI K LEK
Sbjct: 181 DGHPKAVFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPE+QILKDIQ++PLP+NVILALNHS FVKGDQTNFEIEPSFGVEASALYP+V+YTTV
Sbjct: 241 IYVPEDQILKDIQDAPLPLNVILALNHSAFVKGDQTNFEIEPSFGVEASALYPEVRYTTV 300

Query: 301 DESLSRFV 309
           DE LSRFV
Sbjct: 301 DEYLSRFV 308

[Close]

BLAST of Spg033627.1 vs. ExPASy TrEMBL
Match: A0A6J1GJH4 (isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454417 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.1e-157
Identity = 279/308 (90.58%), Postives = 298/308 (96.75%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIK+VDVVIST+GQMQLADQVKIIAAIKEA NVKRFFPSEFG+DVDRL
Sbjct: 61  TGDLYDHESLVKAIKEVDVVISTLGQMQLADQVKIIAAIKEACNVKRFFPSEFGMDVDRL 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKSALA+KAQIRRA+E+EGIPYTYVSSNCF+GYFLP+L QPGLT+PPRDKVVI G
Sbjct: 121 HAVEPAKSALAIKAQIRRAIEREGIPYTYVSSNCFNGYFLPTLSQPGLTAPPRDKVVISG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG PK IFN EEDIGTYTIKAVDDP+TENKILYI+PPKNTYSFNDLVALWE+KI K LEK
Sbjct: 181 DGHPKVIFNLEEDIGTYTIKAVDDPKTENKILYIRPPKNTYSFNDLVALWEKKIDKVLEK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPE+QILKDIQE+PLP+NVILALNHS+FVKGDQTNFEIE SFGVEASALYP+V+YTTV
Sbjct: 241 IYVPEDQILKDIQEAPLPLNVILALNHSVFVKGDQTNFEIEASFGVEASALYPEVRYTTV 300

Query: 301 DESLSRFV 309
           DE LSRFV
Sbjct: 301 DEYLSRFV 308

[Close]

BLAST of Spg033627.1 vs. ExPASy TrEMBL
Match: A0A6J1CT05 (isoflavone reductase-like protein OS=Momordica charantia OX=3673 GN=LOC111013952 PE=4 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 9.4e-154
Identity = 272/308 (88.31%), Postives = 293/308 (95.13%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADP KAKLVENFKN GV LI
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPAKAKLVENFKNSGVHLI 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
           TGDLYDHESLVKAIKQVDVVISTVGQ QLADQVKIIAAIKEAGNVKRF PSEFG+DVDR+
Sbjct: 61  TGDLYDHESLVKAIKQVDVVISTVGQFQLADQVKIIAAIKEAGNVKRFLPSEFGMDVDRV 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
           HAVEPAKSAL +KAQIRRAVE+ GIPYTYVSSNCF+GYFLP+LMQPGLTSPP D+V+IPG
Sbjct: 121 HAVEPAKSALEIKAQIRRAVERAGIPYTYVSSNCFNGYFLPTLMQPGLTSPPTDQVIIPG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG+PKA FN EEDIG+YTIKAVDDP+TENKI+YI+PP NTYSFNDLVALWE KIGK+++K
Sbjct: 181 DGRPKASFNLEEDIGSYTIKAVDDPKTENKIVYIRPPNNTYSFNDLVALWENKIGKSIQK 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPEEQ+LKDIQE+PLPINVIL LNHS+FVKGDQTNFEIE SFGVEASALYPDVKYTTV
Sbjct: 241 LYVPEEQLLKDIQEAPLPINVILGLNHSVFVKGDQTNFEIEASFGVEASALYPDVKYTTV 300

Query: 301 DESLSRFV 309
           D+ LSRFV
Sbjct: 301 DQYLSRFV 308

[Close]

BLAST of Spg033627.1 vs. ExPASy TrEMBL
Match: A0A0A0LL27 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361740 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 3.9e-152
Identity = 270/306 (88.24%), Postives = 287/306 (93.79%), Query Frame = 0


Query: 3   EKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITG 62
           +KSKVLIIGGTGYIGKFVVEASAKA HPTF L+REST+ADPVKAKL+E+FKNLGVK ITG
Sbjct: 6   QKSKVLIIGGTGYIGKFVVEASAKAAHPTFVLVRESTIADPVKAKLLESFKNLGVKFITG 65

Query: 63  DLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLHA 122
           DLYDHE LVKAIKQVDVVISTVGQMQLADQ KI+ AIKEAGNVKRFFPSEFGVDVDRLHA
Sbjct: 66  DLYDHEGLVKAIKQVDVVISTVGQMQLADQSKIVDAIKEAGNVKRFFPSEFGVDVDRLHA 125

Query: 123 VEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPGDG 182
           VEPAKSALAVKA IRRA+EKEGIPYTYV SNCF+GYFLP+LMQPGLTSPP  KVVIPGDG
Sbjct: 126 VEPAKSALAVKANIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVVIPGDG 185

Query: 183 QPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEKVY 242
            PKAIFN EEDIGTYTIKAVDDPRTENKILYIKPP NTYSFNDLVALWE+KIGK LEK+Y
Sbjct: 186 HPKAIFNLEEDIGTYTIKAVDDPRTENKILYIKPPNNTYSFNDLVALWEKKIGKPLEKLY 245

Query: 243 VPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTVDE 302
           VPE QILKDIQE+PLP+NVIL LNHS+FVKGD+TNFEIE SFGVEAS LYPDV+YTTVD+
Sbjct: 246 VPEHQILKDIQEAPLPLNVILGLNHSIFVKGDETNFEIEASFGVEASKLYPDVQYTTVDQ 305

Query: 303 SLSRFV 309
            LSRFV
Sbjct: 306 YLSRFV 311

[Close]

BLAST of Spg033627.1 vs. ExPASy TrEMBL
Match: A0A5A7VHA2 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005640 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 6.7e-152
Identity = 270/305 (88.52%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 4   KSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITGD 63
           KS+VLIIGGTGYIGKFVVEASAKA HPTF LIREST+ADP+KAKLVENFK+LGVK ITGD
Sbjct: 7   KSRVLIIGGTGYIGKFVVEASAKAEHPTFVLIRESTIADPMKAKLVENFKSLGVKFITGD 66

Query: 64  LYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLHAV 123
           LYDHESLVKAIKQVDVVISTVGQMQLADQ KII AIKEAGNVKRFFPSEFGVDVDRLHAV
Sbjct: 67  LYDHESLVKAIKQVDVVISTVGQMQLADQSKIIDAIKEAGNVKRFFPSEFGVDVDRLHAV 126

Query: 124 EPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPGDGQ 183
           EPAKSALAVK+ IRRA+EKEGIPYTYV SNCF+GYFLP+LMQPGLTSPP  KV+IPGDG 
Sbjct: 127 EPAKSALAVKSNIRRAIEKEGIPYTYVVSNCFNGYFLPTLMQPGLTSPPTHKVIIPGDGH 186

Query: 184 PKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEKVYV 243
           PKAIFN EEDIGTYTIKAVDDPRTENKILYI PP NTYSFNDLVALWE+KIGK LEK+YV
Sbjct: 187 PKAIFNLEEDIGTYTIKAVDDPRTENKILYINPPNNTYSFNDLVALWEKKIGKPLEKLYV 246

Query: 244 PEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTVDES 303
           PE QILKDIQE+PLP+NVIL+LNHS+FVKGD+TNFEIEPSFGVEAS LYPDV+YTTVD+ 
Sbjct: 247 PEHQILKDIQEAPLPLNVILSLNHSIFVKGDETNFEIEPSFGVEASKLYPDVQYTTVDQY 306

Query: 304 LSRFV 309
           LSRFV
Sbjct: 307 LSRFV 311

[Close]

BLAST of Spg033627.1 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 457.6 bits (1176), Expect = 7.9e-129
Identity = 227/307 (73.94%), Postives = 261/307 (85.02%), Query Frame = 0


Query: 3   EKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITG 62
           EKSK+L+IGGTGYIGKF+VEASAKAGH TFAL+RE+T++DPVK K V++FK+LGV ++ G
Sbjct: 4   EKSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHG 63

Query: 63  DLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLHA 122
           DL DHESLVKAIKQVDVVISTVG MQ+ DQ KII+AIKEAGNVKRF PSEFGVDVDR  A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSA 123

Query: 123 VEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQ--PGLTSPPRDKVVIPG 182
           VEPAKSA A K QIRR +E EGIPYTY  + CF GY+LP+L+Q  PGLTSPPRDKV I G
Sbjct: 124 VEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILG 183

Query: 183 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 242
           DG  KA+ N EEDI  YTIKAVDDPRT NKILYIKP  NT S N++V LWE+KIGK+LEK
Sbjct: 184 DGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEK 243

Query: 243 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 302
            ++PEEQ+LK IQESP+PINV+L++NH++FV GD TN  IEPSFGVEAS LYPDVKYT+V
Sbjct: 244 THLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKYTSV 303

Query: 303 DESLSRF 308
           DE LS F
Sbjct: 304 DEYLSYF 309

[Close]

BLAST of Spg033627.1 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 452.6 bits (1163), Expect = 2.5e-127
Identity = 220/308 (71.43%), Postives = 255/308 (82.79%), Query Frame = 0


Query: 1   MAEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLI 60
           M  KSK+L IGGTGYIGK++VEASA++GHPT  L+R ST+  P ++  +ENFKNLGV+ +
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 61  TGDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRL 120
            GDL DH SLV +IKQ DVVISTVG   L  Q KII+AIKEAGNVKRFFPSEFG DVDR+
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 121 HAVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQPGLTSPPRDKVVIPG 180
             VEPAKSA A KA+IRR +E EGIPYTYVS N F+GYFLP+L QPG TS PRDKV++ G
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 181 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 240
           DG PKA+FN EEDIGTYTI AVDDPRT NKILYI+PP NTYSFNDLV+LWE KIGK LE+
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 241 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 300
           +YVPEEQ+LK I ES  P+NV+L+L H +FVKG  T+FEIEPSFGVEAS LYPDVKYTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 301 DESLSRFV 309
           DE L+++V
Sbjct: 301 DEILNQYV 308

[Close]

BLAST of Spg033627.1 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 3.8e-123
Identity = 213/308 (69.16%), Postives = 255/308 (82.79%), Query Frame = 0


Query: 2   AEKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLIT 61
           +EKSK+L+IGGTG+IGK ++EAS KAGH T AL+RE++++DP K K V+NFK+ GV L+ 
Sbjct: 3   SEKSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLH 62

Query: 62  GDLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLH 121
           GDL DHESLVKAIKQ DVVISTVG MQ+ DQ KII+AIKEAGNVKRF PSEFG+DVD+  
Sbjct: 63  GDLNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSS 122

Query: 122 AVEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQ--PGLTSPPRDKVVIP 181
           AVEPAKSA   K Q RR +E EGIPYTY+ +N F+GY+LP+L+Q  PGLTSPPRDKV I 
Sbjct: 123 AVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIF 182

Query: 182 GDGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALE 241
           GDG  KA+ N EEDI  YTIKAVDDPRT NK LYI PP NT S N++V LWE+KIGK++E
Sbjct: 183 GDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVE 242

Query: 242 KVYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTT 301
           K+Y+ EEQI K IQESP+P NV+L++NH++FVKGDQTNF IEPSFG EAS LYPD+KYT+
Sbjct: 243 KIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTS 302

Query: 302 VDESLSRF 308
           +DE LS F
Sbjct: 303 IDEYLSYF 310

[Close]

BLAST of Spg033627.1 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 404.1 bits (1037), Expect = 1.0e-112
Identity = 203/321 (63.24%), Postives = 251/321 (78.19%), Query Frame = 0


Query: 3   EKSKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITG 62
           EKSK+L+IGGTGY+G+F+VE SAKAG+PTFAL+RE++++DPVK+K +++FK+LGV ++ G
Sbjct: 4   EKSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHG 63

Query: 63  DLYDHESLVKAIKQVDVVISTVGQMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLHA 122
           DL DHESLVKAIKQVDVVIST+G  Q+ DQ KII+AIKEAGNVKRF P+EFG+DV+R  A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSA 123

Query: 123 VEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQ--PGLTS---------- 182
           VEPAKS  A K QIRRA+E EGIPYTYV SNC +G++L +L+Q   GL S          
Sbjct: 124 VEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFG 183

Query: 183 ----PPRDKVVIPGDGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDL 242
               PPRDKV I GDG  K + N EED+  Y IKAVDD RT NK LYI PP N  S N++
Sbjct: 184 DKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEM 243

Query: 243 VALWEQKIGKALEKVYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGV 302
           V LWE+KIGK+LEK ++ EEQILK IQ   +PI+V  ++NH++FVKGDQT+F IEP FG 
Sbjct: 244 VTLWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGE 303

Query: 303 EASALYPDVKYTTVDESLSRF 308
           EAS LYPDVKYT++DE LS+F
Sbjct: 304 EASVLYPDVKYTSIDEYLSQF 321

[Close]

BLAST of Spg033627.1 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 370.9 bits (951), Expect = 9.7e-103
Identity = 195/308 (63.31%), Postives = 233/308 (75.65%), Query Frame = 0


Query: 5   SKVLIIGGTGYIGKFVVEASAKAGHPTFALIRESTVADPVKAKLVENFKNLGVKLITGDL 64
           SK+L+IG TG IGK +VE SAK+GH TFAL+RE++++DPVKA+LVE FK+LGV ++ G L
Sbjct: 3   SKILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSL 62

Query: 65  YDHESLVKAIKQVDVVISTVG--QMQLADQVKIIAAIKEAGNVKRFFPSEFGVDVDRLHA 124
            D ESLVKAIKQVDVVIS VG  Q ++ +Q  II AIKE+GNVKRF PSEFG DVDR  A
Sbjct: 63  SDKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVA 122

Query: 125 VEPAKSALAVKAQIRRAVEKEGIPYTYVSSNCFSGYFLPSLMQ--PGLTSPPRDKVVIPG 184
           +EP  S    KAQIRRA+E   IPYTYV S CF+G F+P L Q    L SPPRDKV I  
Sbjct: 123 IEPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYD 182

Query: 185 DGQPKAIFNFEEDIGTYTIKAVDDPRTENKILYIKPPKNTYSFNDLVALWEQKIGKALEK 244
            G  KAI N EEDI  YT+KAVDDPRT NKILYI PP    S ND+V LWE+KIGK LEK
Sbjct: 183 TGNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEK 242

Query: 245 VYVPEEQILKDIQESPLPINVILALNHSMFVKGDQTNFEIEPSFGVEASALYPDVKYTTV 304
            YV EE++LK IQES  P++ ++ L H++ VK D T+F I+PSFGVEAS LYP+VKYT+V
Sbjct: 243 TYVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSV 302

Query: 305 DESLSRFV 309
           DE L+RF+
Sbjct: 303 DEFLNRFI 310

The following BLAST results are available for this feature:

BLAST of Spg033627.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002443.1	1.3e-157	90.26	isoflavone reductase-like protein [Cucurbita maxima]	[more]
KAG7020505.1	4.9e-157	90.91	hypothetical protein SDJN02_17190 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022951674.1	8.4e-157	90.58	isoflavone reductase-like protein [Cucurbita moschata]	[more]
XP_023536776.1	1.1e-156	90.26	isoflavone reductase homolog TP7-like [Cucurbita pepo subsp. pepo]	[more]
XP_038885595.1	1.1e-153	89.18	eugenol synthase 2-like [Benincasa hispida]	[more]

BLAST of Spg033627.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6VRE8	7.7e-145	80.19	Phenylcoumaran benzylic ether reductase TP7 OS=Nicotiana tabacum OX=4097 GN=TP7 ...	[more]
B2WSN1	1.7e-144	80.78	Eugenol synthase 1 OS=Petunia hybrida OX=4102 GN=EGS1 PE=1 SV=1	[more]
B2WSN0	1.1e-143	80.58	Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1	[more]
Q9FUW6	2.5e-143	80.84	Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6...	[more]
E1U332	8.8e-141	77.92	Isoflavone reductase-like protein OS=Olea europaea OX=4146 PE=1 SV=1	[more]

BLAST of Spg033627.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KTK3	6.3e-158	90.26	isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111496281 PE...	[more]
A0A6J1GJH4	4.1e-157	90.58	isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454417 ...	[more]
A0A6J1CT05	9.4e-154	88.31	isoflavone reductase-like protein OS=Momordica charantia OX=3673 GN=LOC111013952...	[more]
A0A0A0LL27	3.9e-152	88.24	NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361740 PE=4 S...	[more]
A0A5A7VHA2	6.7e-152	88.52	Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]

BLAST of Spg033627.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75280.1	7.9e-129	73.94	NmrA-like negative transcriptional regulator family protein	[more]
AT4G39230.1	2.5e-127	71.43	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75290.1	3.8e-123	69.16	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G75300.1	1.0e-112	63.24	NmrA-like negative transcriptional regulator family protein	[more]
AT1G19540.1	9.7e-103	63.31	NmrA-like negative transcriptional regulator family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.25.10		coord: 154..303 e-value: 6.6E-143 score: 477.2
None	No IPR available	GENE3D	3.40.50.720		coord: 6..294 e-value: 6.6E-143 score: 477.2
None	No IPR available	PANTHER	PTHR43349:SF76	ISOFLAVONE REDUCTASE HOMOLOG P3-RELATED	coord: 2..308
None	No IPR available	PANTHER	PTHR43349	PINORESINOL REDUCTASE-RELATED	coord: 2..308
None	No IPR available	CDD	cd05259	PCBER_SDR_a	coord: 6..302 e-value: 1.08469E-82 score: 249.142
IPR008030	NmrA-like domain	PFAM	PF05368	NmrA	coord: 7..297 e-value: 7.6E-85 score: 284.1
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 4..277

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg033627	Spg033627	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg033627.1-five_prime_utr	Spg033627.1-five_prime_utr-scaffold13:38766350..38766408	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg033627.1-cds	Spg033627.1-cds-scaffold13:38766409..38766591	CDS
Spg033627.1-cds	Spg033627.1-cds-scaffold13:38766714..38766848	CDS
Spg033627.1-cds	Spg033627.1-cds-scaffold13:38767601..38767838	CDS
Spg033627.1-cds	Spg033627.1-cds-scaffold13:38768122..38768325	CDS
Spg033627.1-cds	Spg033627.1-cds-scaffold13:38768436..38768602	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg033627.1-three_prime_utr	Spg033627.1-three_prime_utr-scaffold13:38768603..38768820	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg033627.1	Spg033627.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009807	lignan biosynthetic process
molecular_function	GO:0016491	oxidoreductase activity