Spg026075.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg026075.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	scaffold7: 2948812 .. 2950796 (+)
Sequence length	681
RNA-Seq Expression	Spg026075.1
Synteny	Spg026075.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACAAAGTGAAACTCTTTCCTTTTTGTAAAGATAAACTTCCTAAGCTTTAAAAAAAAAAACCTTGGATTCGAACTAAATAACCTTAATTTCGGTTAATTTTTGGAGTGGCGAACTAAGGTTAAACATAACAGTAGCTACTTTAAAGGACTCATAATTCCAACTTCTTTTTATGGCAATTAAGCTTTTAATCAAAGTCCAATTTAATTACTCTCACAATCATGACTCATCAGTTGCTTTTGAATTACGCTAAAAAAAAAAAAAGTATTAACCCCAAAAAAACGGGTGTAGGGATCAAGTTCTGAATAAAGTTTCTAATAATTTAATCCCCTAATTTCTTCATTATCCCATTTGCCAGCCTGCTCTCTCTTTTGTGGAATCTCCTGAGAAATTCCAATATTTTTCATTTTTTTTCCTCTAATTATGCTCAAGAAGACATCGAAAATAAAGAATTAATTGAAATTAAAATGTCAAATTGAAGAAAACAGCTGGCTTTGGCGAAGAGGATCAATTGAAGCTGATGGGGACCTACTGTTTCATGACGAGCACGTTCGTTAATAAGCCCCCACAAAAAATAAATAATAAATTTAAAATAAAAAGGAAACCAAACCAACTGAAATGATTACAAAAGCAAACAAAAACAAAAAAAAACAAAAAAAAAAAAAAAAAGAAAAGAAAAAAGAAAAAGAAGAAACTCACAATCACAATATCTCTTTACCGACAAATAGAAATCTGTTCGATCAGAGTCGAATTCTAACGAAAACGAAAGCCTTTTCATCTTCTTCTTCTGTCTTCAGCAAATCCCATCTCCAGAAATGGGGAAAACCGGGGCGAAATTGCCGAGTTTCTGCCTGAACCGGATCCGGCCTCACGTTCGAGTGCCTATTCAGTCCAAACCGGACTCTGTTTCTGTGAAAACCGGCCAGAAGAAGGCTGATGATTTCGAAGCGAAGAAATGTGATGACAATAAAACGTTGGGAATTGGAAGGAAGATCATGATTGTGGTTGATTCCAGCTTTGAAGCTGAAGGAGCTCTGCAATGGGCGCTCTCACACACTGTTCAGAATCAAGACAAGATTCTTCTTCTCCATGTCACCAAACCTTCAAAAAGAGGTCCAAATCTTCCATCCACATTGATTCATTTACTTAGAATTTTAAGAATTGGGTTGATTTCATGAACTTTTGTTACAGGGGAAGGCCCCAGCAAGGAGACGGCTCCAAGAGCATTTGAAGTGGTTCACTCCATGAGAAATTTATGTCAACTGAAGAGACCCGAGGTAAATTAGATGGGATTTTGAGCAAATGGGGTGGATTAAAACAGGATTTTTAATGATGGGTTATGAAAATTTTGATTGTTTATTAGGTGGAAATCGAGGTTGCAGTGGTGGAAGGGAAAGAGAAAGGGCCAGTGATAGTGGAAGAAGCGAGGAAGCAAGGGGCGTCGTTGCTGGTTTTGGGGCAGAAGAAACGGTCGACGACGTGGCGGCTTCTGATGGTGTGGGCCGGCCACCGGTTTGGCGGCGGCGGCGGCGGTGGCGCGGTGGAGTATTGTATTCAGAATGCGAACTGCATGGCGATTGCAGTGAGGAGGAAGAGCAAGAAATTGGGTGGCTATTTGATTACAACGAAGCGTCAAAAGGATTTCTGGCTTTTGGCTTAATTAGAATTAGAATTAGAAACAAGAATTTGGTTTGAATCAAAATGTTTGTGGTTTGAGAGATGTAATGTAAATAATGTAATTGAGAATTGTGAATTGTAAGTGATTTAGATTTTTTTTTTGGGGGTTTGGAAAAAAGTGATGAATATGTGGGGCTTGAATGTTGGGTAGAGTGGCACTCATGAAGGCTCCAAAAGGTGTATGGGTGGGCAGGGCATGTGTCTGAAACTGTTGGAATGTGATCATTTGGGAATCCCTATATGGCTTGAGGCAACTTTGACTGTACAACAGTTTCCCTCTAATTTATAATTTCTTGACAGATTGATTTGG

mRNA sequence

ATGGGGAAAACCGGGGCGAAATTGCCGAGTTTCTGCCTGAACCGGATCCGGCCTCACGTTCGAGTGCCTATTCAGTCCAAACCGGACTCTGTTTCTGTGAAAACCGGCCAGAAGAAGGCTGATGATTTCGAAGCGAAGAAATGTGATGACAATAAAACGTTGGGAATTGGAAGGAAGATCATGATTGTGGTTGATTCCAGCTTTGAAGCTGAAGGAGCTCTGCAATGGGCGCTCTCACACACTGTTCAGAATCAAGACAAGATTCTTCTTCTCCATGTCACCAAACCTTCAAAAAGAGGGGAAGGCCCCAGCAAGGAGACGGCTCCAAGAGCATTTGAAGTGGTTCACTCCATGAGAAATTTATGTCAACTGAAGAGACCCGAGGTGGAAATCGAGGTTGCAGTGGTGGAAGGGAAAGAGAAAGGGCCAGTGATAGTGGAAGAAGCGAGGAAGCAAGGGGCGTCGTTGCTGGTTTTGGGGCAGAAGAAACGGTCGACGACGTGGCGGCTTCTGATGGTGTGGGCCGGCCACCGGTTTGGCGGCGGCGGCGGCGGTGGCGCGGTGGAGTATTGTATTCAGAATGCGAACTGCATGGCGATTGCAGTGAGGAGGAAGAGCAAGAAATTGGGTGGCTATTTGATTACAACGAAGCGTCAAAAGGATTTCTGGCTTTTGGCTTAA

Coding sequence (CDS)

ATGGGGAAAACCGGGGCGAAATTGCCGAGTTTCTGCCTGAACCGGATCCGGCCTCACGTTCGAGTGCCTATTCAGTCCAAACCGGACTCTGTTTCTGTGAAAACCGGCCAGAAGAAGGCTGATGATTTCGAAGCGAAGAAATGTGATGACAATAAAACGTTGGGAATTGGAAGGAAGATCATGATTGTGGTTGATTCCAGCTTTGAAGCTGAAGGAGCTCTGCAATGGGCGCTCTCACACACTGTTCAGAATCAAGACAAGATTCTTCTTCTCCATGTCACCAAACCTTCAAAAAGAGGGGAAGGCCCCAGCAAGGAGACGGCTCCAAGAGCATTTGAAGTGGTTCACTCCATGAGAAATTTATGTCAACTGAAGAGACCCGAGGTGGAAATCGAGGTTGCAGTGGTGGAAGGGAAAGAGAAAGGGCCAGTGATAGTGGAAGAAGCGAGGAAGCAAGGGGCGTCGTTGCTGGTTTTGGGGCAGAAGAAACGGTCGACGACGTGGCGGCTTCTGATGGTGTGGGCCGGCCACCGGTTTGGCGGCGGCGGCGGCGGTGGCGCGGTGGAGTATTGTATTCAGAATGCGAACTGCATGGCGATTGCAGTGAGGAGGAAGAGCAAGAAATTGGGTGGCTATTTGATTACAACGAAGCGTCAAAAGGATTTCTGGCTTTTGGCTTAA

Protein sequence

MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADDFEAKKCDDNKTLGIGRKIMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMRNLCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGHRFGGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA

Homology

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BLAST of Spg026075.1 vs. NCBI nr
Match: XP_022945318.1 (uncharacterized protein LOC111449594 [Cucurbita moschata])

HSP 1 Score: 380.2 bits (975), Expect = 1.3e-101
Identity = 193/228 (84.65%), Postives = 207/228 (90.79%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADDFEAKKCDDNKTLGIGRKI 60
           MGKTG KLPSFCLNRIR HVRVPIQSKPDSVSVKTG++K  +F+         LGIGRKI
Sbjct: 1   MGKTGGKLPSFCLNRIRSHVRVPIQSKPDSVSVKTGERKGGEFDESNGGGKPALGIGRKI 60

Query: 61  MIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMRN 120
           MIVVDS+ EAEGALQWALS+TVQNQD I+LLH+T PSK+GEG SKETAPR +E+VHSMRN
Sbjct: 61  MIVVDSTIEAEGALQWALSNTVQNQDMIVLLHLTNPSKKGEG-SKETAPRTYELVHSMRN 120

Query: 121 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ-KKRSTTWRLLMVWAGHRF 180
           LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ KKRSTTWRLLM+WAGHR+
Sbjct: 121 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQKKKRSTTWRLLMIWAGHRW 180

Query: 181 GGGG-GGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           GGGG GGG VEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 181 GGGGDGGGTVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227

[Close]

BLAST of Spg026075.1 vs. NCBI nr
Match: XP_023542429.1 (uncharacterized protein LOC111802335 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.2 bits (975), Expect = 1.3e-101
Identity = 194/229 (84.72%), Postives = 207/229 (90.39%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADDFEAKKCDDNKTLGIGRKI 60
           MGKTG KLPSFCLNRIR HVRVPIQSKPDSVSVKTG++K  +F+         LGIGRKI
Sbjct: 64  MGKTGGKLPSFCLNRIRSHVRVPIQSKPDSVSVKTGERKGGEFDESNGGGKPALGIGRKI 123

Query: 61  MIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMRN 120
           MIVVDS+ EAEGALQWALS+TVQNQD I+LLH+TKPSK+GEG SKETAPR +E+VHSMRN
Sbjct: 124 MIVVDSTIEAEGALQWALSNTVQNQDMIVLLHLTKPSKKGEG-SKETAPRTYELVHSMRN 183

Query: 121 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ-KKRSTTWRLLMVWAGHRF 180
           LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ KKR TTWRLLMVWAGHR+
Sbjct: 184 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQKKKRPTTWRLLMVWAGHRW 243

Query: 181 GGG--GGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           GGG  GGGG VEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 244 GGGGDGGGGTVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 291

[Close]

BLAST of Spg026075.1 vs. NCBI nr
Match: KAG6601483.1 (hypothetical protein SDJN03_06716, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 379.0 bits (972), Expect = 2.8e-101
Identity = 197/231 (85.28%), Postives = 208/231 (90.04%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADD-FEAKKCDDNKTLGIGRK 60
           MGKTG KLPSFCLNRIRPHVRVPIQSK DSVSVKTG KKADD  E  +      LG GRK
Sbjct: 1   MGKTGTKLPSFCLNRIRPHVRVPIQSKLDSVSVKTGPKKADDCVEGGEVGAKPGLGNGRK 60

Query: 61  IMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMR 120
           IMIVVDS+ EAEGALQWALSHTVQNQDKI+LLHV KPS++GEG SKET+PRA+E+VHSMR
Sbjct: 61  IMIVVDSTIEAEGALQWALSHTVQNQDKIVLLHVIKPSRKGEGLSKETSPRAYELVHSMR 120

Query: 121 NLCQLKRPEVEIEVAVVE-GKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGHR 180
           NLCQLKRPEVE+EVAVVE GKEKG VIVEEARKQ ASLLVLGQKKRSTTWRLLMVWAGHR
Sbjct: 121 NLCQLKRPEVEVEVAVVEGGKEKGSVIVEEARKQEASLLVLGQKKRSTTWRLLMVWAGHR 180

Query: 181 F---GGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           +   GGGGGGGAVEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 181 WGGGGGGGGGGAVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 231

[Close]

BLAST of Spg026075.1 vs. NCBI nr
Match: KAG7032262.1 (hypothetical protein SDJN02_06306 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 379.0 bits (972), Expect = 2.8e-101
Identity = 197/231 (85.28%), Postives = 208/231 (90.04%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADD-FEAKKCDDNKTLGIGRK 60
           MGKTG KLPSFCLNRIRPHVRVPIQSK DSVSVKTG KKADD  E  +      LG GRK
Sbjct: 96  MGKTGTKLPSFCLNRIRPHVRVPIQSKLDSVSVKTGPKKADDCVEGGEVGAKPGLGNGRK 155

Query: 61  IMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMR 120
           IMIVVDS+ EAEGALQWALSHTVQNQDKI+LLHV KPS++GEG SKET+PRA+E+VHSMR
Sbjct: 156 IMIVVDSTIEAEGALQWALSHTVQNQDKIVLLHVIKPSRKGEGLSKETSPRAYELVHSMR 215

Query: 121 NLCQLKRPEVEIEVAVVE-GKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGHR 180
           NLCQLKRPEVE+EVAVVE GKEKG VIVEEARKQ ASLLVLGQKKRSTTWRLLMVWAGHR
Sbjct: 216 NLCQLKRPEVEVEVAVVEGGKEKGSVIVEEARKQEASLLVLGQKKRSTTWRLLMVWAGHR 275

Query: 181 F---GGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           +   GGGGGGGAVEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 276 WGGGGGGGGGGAVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 326

[Close]

BLAST of Spg026075.1 vs. NCBI nr
Match: XP_022956495.1 (uncharacterized protein LOC111458216 [Cucurbita moschata])

HSP 1 Score: 377.1 bits (967), Expect = 1.1e-100
Identity = 196/232 (84.48%), Postives = 207/232 (89.22%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADD-FEAKKCDDNKTLGIGRK 60
           MGKTG KLPSFCLNRIRPHVRVPIQSK DSVSVKTG KKADD  E  +      LG GRK
Sbjct: 96  MGKTGTKLPSFCLNRIRPHVRVPIQSKLDSVSVKTGPKKADDCVEGGEVGAKPGLGNGRK 155

Query: 61  IMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMR 120
           IMIVVDS+ EAEGALQWALSHTVQNQDKI+LLHV KPS++GEG SKET+PRA+E+VHSMR
Sbjct: 156 IMIVVDSTIEAEGALQWALSHTVQNQDKIVLLHVIKPSRKGEGLSKETSPRAYELVHSMR 215

Query: 121 NLCQLKRPEVEIEVAVVE-GKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGHR 180
           NLCQLKRPEVE+EVAVVE GKEKG VIVEEARKQ ASLLVLGQKKRSTTWRLLMVWAGHR
Sbjct: 216 NLCQLKRPEVEVEVAVVEGGKEKGSVIVEEARKQEASLLVLGQKKRSTTWRLLMVWAGHR 275

Query: 181 F----GGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           +    GGGGGGG VEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 276 WGGGGGGGGGGGVVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 327

[Close]

BLAST of Spg026075.1 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.9e-05
Identity = 45/189 (23.81%), Postives = 81/189 (42.86%), Query Frame = 0


Query: 53  TLGIGRKIMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPS-------------KR 112
           T G  RKI + VD S E+  A++WA+ H ++  D ++LLHV+  S              +
Sbjct: 43  TAGARRKIGVAVDLSEESSFAVRWAVDHYIRPGDAVVLLHVSPTSVLFGADWGPLPLKTQ 102

Query: 113 GEGPSKETAP--RAFEVVHSMRNLCQLKRPEVEI----EVAVVEGKEKGPVIVEEARKQG 172
            E P+ +  P    F+   S + +  L +P  E+    ++ +V+  +    +  E  + G
Sbjct: 103 IEDPNAQPQPSQEDFDAFTSTK-VADLAKPLKELGFPYKIHIVKDHDMRERLCLEIERLG 162

Query: 173 ASLLVLG------QKKRSTTWRLLMVWAGHRFGGGGGGGAVEYCIQNANCMAIAVRRKSK 217
            S +++G      +KKR +  +L              G   +YC+ +  C  + VR    
Sbjct: 163 LSAVIMGSRGFGAEKKRGSDGKL--------------GSVSDYCVHHCVCPVVVVRYPDD 216

[Close]

BLAST of Spg026075.1 vs. ExPASy TrEMBL
Match: A0A6J1G0H0 (uncharacterized protein LOC111449594 OS=Cucurbita moschata OX=3662 GN=LOC111449594 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.1e-102
Identity = 193/228 (84.65%), Postives = 207/228 (90.79%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADDFEAKKCDDNKTLGIGRKI 60
           MGKTG KLPSFCLNRIR HVRVPIQSKPDSVSVKTG++K  +F+         LGIGRKI
Sbjct: 1   MGKTGGKLPSFCLNRIRSHVRVPIQSKPDSVSVKTGERKGGEFDESNGGGKPALGIGRKI 60

Query: 61  MIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMRN 120
           MIVVDS+ EAEGALQWALS+TVQNQD I+LLH+T PSK+GEG SKETAPR +E+VHSMRN
Sbjct: 61  MIVVDSTIEAEGALQWALSNTVQNQDMIVLLHLTNPSKKGEG-SKETAPRTYELVHSMRN 120

Query: 121 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ-KKRSTTWRLLMVWAGHRF 180
           LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ KKRSTTWRLLM+WAGHR+
Sbjct: 121 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQKKKRSTTWRLLMIWAGHRW 180

Query: 181 GGGG-GGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           GGGG GGG VEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 181 GGGGDGGGTVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227

[Close]

BLAST of Spg026075.1 vs. ExPASy TrEMBL
Match: A0A6J1GXX9 (uncharacterized protein LOC111458216 OS=Cucurbita moschata OX=3662 GN=LOC111458216 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.2e-101
Identity = 196/232 (84.48%), Postives = 207/232 (89.22%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADD-FEAKKCDDNKTLGIGRK 60
           MGKTG KLPSFCLNRIRPHVRVPIQSK DSVSVKTG KKADD  E  +      LG GRK
Sbjct: 96  MGKTGTKLPSFCLNRIRPHVRVPIQSKLDSVSVKTGPKKADDCVEGGEVGAKPGLGNGRK 155

Query: 61  IMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMR 120
           IMIVVDS+ EAEGALQWALSHTVQNQDKI+LLHV KPS++GEG SKET+PRA+E+VHSMR
Sbjct: 156 IMIVVDSTIEAEGALQWALSHTVQNQDKIVLLHVIKPSRKGEGLSKETSPRAYELVHSMR 215

Query: 121 NLCQLKRPEVEIEVAVVE-GKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGHR 180
           NLCQLKRPEVE+EVAVVE GKEKG VIVEEARKQ ASLLVLGQKKRSTTWRLLMVWAGHR
Sbjct: 216 NLCQLKRPEVEVEVAVVEGGKEKGSVIVEEARKQEASLLVLGQKKRSTTWRLLMVWAGHR 275

Query: 181 F----GGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           +    GGGGGGG VEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 276 WGGGGGGGGGGGVVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 327

[Close]

BLAST of Spg026075.1 vs. ExPASy TrEMBL
Match: A0A6J1IT49 (uncharacterized protein LOC111478423 OS=Cucurbita maxima OX=3661 GN=LOC111478423 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.2e-100
Identity = 195/231 (84.42%), Postives = 207/231 (89.61%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADD-FEAKKCDDNKTLGIGRK 60
           MGKTG KLPSFCLNRIRPHVRVPIQSK DSVSVKTG KKADD     +      LG GRK
Sbjct: 96  MGKTGTKLPSFCLNRIRPHVRVPIQSKLDSVSVKTGPKKADDCVVGGEVGAKPGLGNGRK 155

Query: 61  IMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMR 120
           IMIV+DS+ EAEGALQWALSHTVQNQDKI+LLHV KPS++GEG SKET+PRA+E+VHSMR
Sbjct: 156 IMIVIDSTIEAEGALQWALSHTVQNQDKIVLLHVIKPSRKGEGLSKETSPRAYELVHSMR 215

Query: 121 NLCQLKRPEVEIEVAVVE-GKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGHR 180
           NLCQLKRPEVE+EVAVVE GKEKG VIVEEARKQ ASLLVLGQKKRSTTWRLLMVWAGHR
Sbjct: 216 NLCQLKRPEVEVEVAVVEGGKEKGSVIVEEARKQEASLLVLGQKKRSTTWRLLMVWAGHR 275

Query: 181 FGGG---GGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           +GGG   GGGGAVEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 276 WGGGGGVGGGGAVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 326

[Close]

BLAST of Spg026075.1 vs. ExPASy TrEMBL
Match: A0A6J1HXT3 (universal stress protein PHOS32-like OS=Cucurbita maxima OX=3661 GN=LOC111467624 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.7e-99
Identity = 193/230 (83.91%), Postives = 204/230 (88.70%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTGQKKADDFEAKKCDDNKTLGIGRKI 60
           MGKTG KLPSF LNRIR HVRVPIQSKPDSVSVKTG++   +F          LGIGRKI
Sbjct: 64  MGKTGGKLPSFFLNRIRSHVRVPIQSKPDSVSVKTGERNGGEFAESNGGAKPALGIGRKI 123

Query: 61  MIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMRN 120
           MIVVDS+ EAEGALQWALS+TVQNQD I+LLH+T PSK+GEG SKETAPRA+E+VHSMRN
Sbjct: 124 MIVVDSTIEAEGALQWALSNTVQNQDMIVLLHLTNPSKKGEG-SKETAPRAYELVHSMRN 183

Query: 121 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ-KKRSTTWRLLMVWAGHRF 180
           LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQ KKRSTTWRLLMVWAGHR 
Sbjct: 184 LCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQKKKRSTTWRLLMVWAGHRR 243

Query: 181 GG---GGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           GG   GGGGG VEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 244 GGGGDGGGGGTVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 292

[Close]

BLAST of Spg026075.1 vs. ExPASy TrEMBL
Match: A0A5A7SU40 (Usp domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00200 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 4.1e-98
Identity = 192/235 (81.70%), Postives = 202/235 (85.96%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTG-QKKADDFEAKKCD------DNK- 60
           MGKTGAKLPSFCLNRIRPHVRVPIQSKPD VS KTG  KK DD   +K D       NK 
Sbjct: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDYVSAKTGPNKKIDDCSDEKNDHIGVDQSNKG 60

Query: 61  TLGIGRKIMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAF 120
            +GIGRKIMIVVDS+ EAEGAL WALSHTVQ QD ILLLHVTKPS +GEGP+KETAPRA+
Sbjct: 61  VMGIGRKIMIVVDSTIEAEGALHWALSHTVQIQDNILLLHVTKPSSKGEGPNKETAPRAY 120

Query: 121 EVVHSMRNLCQLKRPEVEIEVAVVE-GKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLL 180
           E+VHSMR LCQLKRPEVE EV VVE GKEKG VIVEEARK+G SLLVLGQKKRSTTWRLL
Sbjct: 121 ELVHSMRTLCQLKRPEVETEVVVVEGGKEKGAVIVEEARKRGVSLLVLGQKKRSTTWRLL 180

Query: 181 MVWAGHRFGGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           MVWAG R+GGG  GG VEYCIQNA+CMAIAVRRKSKKLGGYLITTKRQKDFWLLA
Sbjct: 181 MVWAGQRWGGGSSGGVVEYCIQNASCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 235

[Close]

BLAST of Spg026075.1 vs. TAIR 10
Match: AT1G69080.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.9e-48
Identity = 117/248 (47.18%), Postives = 149/248 (60.08%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTG---QKKADDFEAKKCDDNKTLGIG 60
           MGK G     F ++R+R +VRV    +P + + + G   +      E K          G
Sbjct: 1   MGKKG---EGFFVSRLRANVRV----QPPTTTSQHGCSHEPPTTSIEIK----------G 60

Query: 61  RKIMIVVDSSFEAEGALQWALSHTVQNQDKILLLHV--TKPSKRGEGPSKE--------- 120
           R+I++VVDS  EA+ AL W LSH  Q QD ILLLH    K S+ G+  +KE         
Sbjct: 61  RRIIVVVDSCSEAKNALLWTLSHCAQPQDSILLLHFLKAKTSQSGDLANKEEGEDESCDK 120

Query: 121 -TAPRAFEVVHSMRNLCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQKKRS 180
            T  RA + V +++ +C+LKRPEV+ EV  V+G EKGP IV+EAR++ ASLLVLGQKK+ 
Sbjct: 121 PTTSRADKKVSALKTMCELKRPEVKTEVVFVKGDEKGPTIVKEAREREASLLVLGQKKQH 180

Query: 181 TTWRLLMVWAG-------HRFGGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKR 227
            TWRLLMVWA        H F        VEYCI N+ CMAIAVR++ KKLGGY +TTKR
Sbjct: 181 ATWRLLMVWASQARPVTKHDF--------VEYCINNSPCMAIAVRKRGKKLGGYTLTTKR 223

[Close]

BLAST of Spg026075.1 vs. TAIR 10
Match: AT2G03720.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 4.2e-47
Identity = 95/169 (56.21%), Postives = 125/169 (73.96%), Query Frame = 0


Query: 61  MIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKP--SKRGEGPSKETAPRAFEVVHSM 120
           M+VVD++ + + ALQWAL+H VQ++D I LLHVT+    +  +   +E   RA E+VH +
Sbjct: 1   MVVVDTTSQTKNALQWALTHCVQDEDNITLLHVTRTPVGQAIDETQRERNSRAHELVHPL 60

Query: 121 RNLCQLKRPEVEIEVAVVE-GKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGH 180
           +N CQLK+P V+ E+ VVE  +EKG  IVEE++KQGA +LVLGQ+KR++ WR++  W   
Sbjct: 61  KNFCQLKKPNVKTEIVVVETAEEKGKTIVEESKKQGAGVLVLGQRKRTSKWRVIWKW--- 120

Query: 181 RFGGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
           R  GG GGG VEYCI N++CMAIAVR+KS   GGYLITTKR KDFWLLA
Sbjct: 121 RTKGGMGGGVVEYCIHNSDCMAIAVRKKSNN-GGYLITTKRHKDFWLLA 165

[Close]

BLAST of Spg026075.1 vs. TAIR 10
Match: AT5G17390.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 4.0e-45
Identity = 90/204 (44.12%), Postives = 138/204 (67.65%), Query Frame = 0


Query: 25  QSKPDSVSVKTGQKKADDFEAKKCDDNKTLGIGRKIMIVVDSSFEAEGALQWALSHTVQN 84
           Q    S ++  G+ K +   A +  + +  G G ++M+VVD +  + GAL+WA++HT+Q 
Sbjct: 85  QDDAKSFNIGGGEGKKEAVAAVEAVEEEAEG-GNRVMVVVDKALASTGALEWAITHTLQP 144

Query: 85  QDKILLLHVTKPSKRGEGPSKETAPRAFEVVHSMRNLCQLKRPEVEIEVAVVEG--KEKG 144
           QD + LL+  KP ++ +  +++   +  E+VH+++ LCQ KRP +E+E+  +EG  K+KG
Sbjct: 145 QDTLFLLYFAKPFRKSKRKNRKREVKTDELVHTLKKLCQTKRPGIEVEIRRLEGKDKDKG 204

Query: 145 PVIVEEARKQGASLLVLGQKKRSTTWRLLMVWAGHRFGGGGGGGAVEYCIQNANCMAIAV 204
             IVEE++KQ  SLLV+GQ+K+   WRLL  WA  R    G  G ++YC++NA+CM IAV
Sbjct: 205 QKIVEESKKQQVSLLVVGQEKKPPVWRLLKRWAWKR--RRGHEGVLKYCLENASCMTIAV 264

Query: 205 RRKSKKLGGYLITTKRQKDFWLLA 227
           + K++KLGGYLITTKR K+FWLLA
Sbjct: 265 KPKNRKLGGYLITTKRHKNFWLLA 285

[Close]

BLAST of Spg026075.1 vs. TAIR 10
Match: AT3G03290.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 7.5e-44
Identity = 82/172 (47.67%), Postives = 125/172 (72.67%), Query Frame = 0


Query: 57  GRKIMIVVDSSFEAEGALQWALSHTVQNQDKILLLHVTKPSKRGEGPSKETAPRAFEVVH 116
           G ++M+VVD    + GAL+WAL HT+Q+QD + LL+ +KP ++G+  ++++  +  E+VH
Sbjct: 105 GNRVMVVVDKVIASTGALEWALKHTLQSQDYLFLLYFSKPFRKGKRKNRKSEVKTDELVH 164

Query: 117 SMRNLCQLKRPEVEIEVAVVEG--KEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVW 176
           +++ LCQ KRP +E+E+  ++G  KEKG  IVEEA++Q  SLLV+G++K+   WRLL  W
Sbjct: 165 TLKKLCQTKRPGIEVEIRRLQGKEKEKGEKIVEEAKEQQVSLLVVGKEKKPPVWRLLKRW 224

Query: 177 AGHRFGGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
              +    G  G ++YC++ A+CM IAV+ K++KLGGYLITTKR K+FWLLA
Sbjct: 225 GWKK--RRGRAGTLKYCLEKASCMTIAVKPKNRKLGGYLITTKRHKNFWLLA 274

[Close]

BLAST of Spg026075.1 vs. TAIR 10
Match: AT1G69080.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 7.8e-41
Identity = 108/238 (45.38%), Postives = 136/238 (57.14%), Query Frame = 0


Query: 1   MGKTGAKLPSFCLNRIRPHVRVPIQSKPDSVSVKTG---QKKADDFEAKKCDDNKTLGIG 60
           MGK G     F ++R+R +VRV    +P + + + G   +      E K          G
Sbjct: 1   MGKKG---EGFFVSRLRANVRV----QPPTTTSQHGCSHEPPTTSIEIK----------G 60

Query: 61  RKIMIVVDSSFEAEGALQWALSHTVQNQDKILLLHV--TKPSKRGEGPSKETAPRAFEVV 120
           R+I++VVDS  EA+ AL W LSH  Q QD ILLLH    K S+ G+  +KE      E  
Sbjct: 61  RRIIVVVDSCSEAKNALLWTLSHCAQPQDSILLLHFLKAKTSQSGDLANKEEG----EDE 120

Query: 121 HSMRNLCQLKRPEVEIEVAVVEGKEKGPVIVEEARKQGASLLVLGQKKRSTTWRLLMVWA 180
              +        +V+ EV  V+G EKGP IV+EAR++ ASLLVLGQKK+  TWRLLMVWA
Sbjct: 121 SCDKPTTSRADKKVKTEVVFVKGDEKGPTIVKEAREREASLLVLGQKKQHATWRLLMVWA 180

Query: 181 G-------HRFGGGGGGGAVEYCIQNANCMAIAVRRKSKKLGGYLITTKRQKDFWLLA 227
                   H F        VEYCI N+ CMAIAVR++ KKLGGY +TTKR KDFWLLA
Sbjct: 181 SQARPVTKHDF--------VEYCINNSPCMAIAVRKRGKKLGGYTLTTKRHKDFWLLA 209

The following BLAST results are available for this feature:

BLAST of Spg026075.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945318.1	1.3e-101	84.65	uncharacterized protein LOC111449594 [Cucurbita moschata]	[more]
XP_023542429.1	1.3e-101	84.72	uncharacterized protein LOC111802335 [Cucurbita pepo subsp. pepo]	[more]
KAG6601483.1	2.8e-101	85.28	hypothetical protein SDJN03_06716, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7032262.1	2.8e-101	85.28	hypothetical protein SDJN02_06306 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022956495.1	1.1e-100	84.48	uncharacterized protein LOC111458216 [Cucurbita moschata]	[more]

BLAST of Spg026075.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYN9	3.9e-05	23.81	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]

BLAST of Spg026075.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0H0	6.1e-102	84.65	uncharacterized protein LOC111449594 OS=Cucurbita moschata OX=3662 GN=LOC1114495...	[more]
A0A6J1GXX9	5.2e-101	84.48	uncharacterized protein LOC111458216 OS=Cucurbita moschata OX=3662 GN=LOC1114582...	[more]
A0A6J1IT49	1.2e-100	84.42	uncharacterized protein LOC111478423 OS=Cucurbita maxima OX=3661 GN=LOC111478423...	[more]
A0A6J1HXT3	1.7e-99	83.91	universal stress protein PHOS32-like OS=Cucurbita maxima OX=3661 GN=LOC111467624...	[more]
A0A5A7SU40	4.1e-98	81.70	Usp domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Spg026075.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69080.1	2.9e-48	47.18	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT2G03720.1	4.2e-47	56.21	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT5G17390.1	4.0e-45	44.12	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03290.1	7.5e-44	47.67	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT1G69080.2	7.8e-41	45.38	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 57..210 e-value: 3.0E-20 score: 74.9
IPR006016	UspA	PFAM	PF00582	Usp	coord: 58..203 e-value: 1.7E-23 score: 83.7
None	No IPR available	PANTHER	PTHR47000	ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN	coord: 1..226
None	No IPR available	CDD	cd00293	USP_Like	coord: 59..172 e-value: 2.43104E-13 score: 62.7726
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 58..205

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg026075	Spg026075	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg026075.1-five_prime_utr	Spg026075.1-five_prime_utr-scaffold7:2948812..2949624	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg026075.1-cds	Spg026075.1-cds-scaffold7:2949625..2949922	CDS
Spg026075.1-cds	Spg026075.1-cds-scaffold7:2950001..2950086	CDS
Spg026075.1-cds	Spg026075.1-cds-scaffold7:2950173..2950469	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg026075.1-three_prime_utr	Spg026075.1-three_prime_utr-scaffold7:2950470..2950796	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg026075.1	Spg026075.1-protein	polypeptide