Spg025033.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg025033.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	GRF-type domain-containing protein
Location	scaffold12: 9729199 .. 9730174 (+)
Sequence length	384
RNA-Seq Expression	Spg025033.1
Synteny	Spg025033.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGTTAAGTGACAGTTTGGTCCCTGAGTTTTAAAACTCAACAAATATACCCCTGAGTTTTTAGATAAGCAGCCAAATGGTCCCTGCCGTTCAATTTCCGTGAAGTCACTAACGGAGAGGTGATGTGGCGCCCATTAAAATGACATGGCGTCCTGATGTGGTTACTGACGTGGCAATTTTCAAGTTTTCACTGGATGAGATGGCAATTTTAAAGTCCACGTGTGTTCCCAAAAATCAAACCTAAAAAAAAAAAAGAAAATCGAACAGATCTCCTTCGTGTTTCCCCTTTTCGTTCGTCTTCTTCATCCATCGATTAGGGTTCTTAAAATTACTCCAAATTTGAAGCTGGATGTCACGAGATATGCCACAGAAACTTTCGAAAATTGCAATGGATAACTTTGAAGAGTCCATAAGAGGCGATAATAGTGAAATTAAGAACCCTAGCTACTGTCACTGTGGGTTACGAGCTCGATTAATCACTTCATGGACAAAGAGGAACCCTGGAAGAAGATTCTATGGATGTCCTGGTTACAAGGTATGTATTCTTTTGTTTATGACTAGTTTATTCATTTTTCGGATGTTTGCAATTTGTTTGCAATTTGTTTTACATACTCGACTGCTCGTCTTCTCTGTTGTGTAGGGGAATGGTTCGGGATGTGGGTTTTTTGATTGGTGTGATCCAGAAGTATCAGAACACAGCAAAGAAGTGATTATCAAACTAAGAGATGAAGTTGGTCAGTTGAAGAAGATTGCACAAAACTTGCGCCGTTTGGAGTGGTTGTATCGTGTAATAATTATAGTATTGGTGGGCAATTTAGTGATTGTATTTGGGTTTTGATTGGGAAGAACTGTAAAAATTATGGATACATGAAGGTTGTGATGATTTTTTTTTTTTTTTTTTGTGTTCTTGGAAAGATTATCTATGTTGTAGCTATGTTCTTAATGTTATGGAATTGTGATGGAGTTTGCTTCAGCG

mRNA sequence

ATGTCACGAGATATGCCACAGAAACTTTCGAAAATTGCAATGGATAACTTTGAAGAGTCCATAAGAGGCGATAATAGTGAAATTAAGAACCCTAGCTACTGTCACTGTGGGTTACGAGCTCGATTAATCACTTCATGGACAAAGAGGAACCCTGGAAGAAGATTCTATGGATGTCCTGGTTACAAGGGGAATGGTTCGGGATGTGGGTTTTTTGATTGGTGTGATCCAGAAGTATCAGAACACAGCAAAGAAGTGATTATCAAACTAAGAGATGAAGTTGGTCAGTTGAAGAAGATTGCACAAAACTTGCGCCGTTTGGAGTGGTTGTATCGTGTAATAATTATAGTATTGGTGGGCAATTTAGTGATTGTATTTGGGTTTTGA

Coding sequence (CDS)

ATGTCACGAGATATGCCACAGAAACTTTCGAAAATTGCAATGGATAACTTTGAAGAGTCCATAAGAGGCGATAATAGTGAAATTAAGAACCCTAGCTACTGTCACTGTGGGTTACGAGCTCGATTAATCACTTCATGGACAAAGAGGAACCCTGGAAGAAGATTCTATGGATGTCCTGGTTACAAGGGGAATGGTTCGGGATGTGGGTTTTTTGATTGGTGTGATCCAGAAGTATCAGAACACAGCAAAGAAGTGATTATCAAACTAAGAGATGAAGTTGGTCAGTTGAAGAAGATTGCACAAAACTTGCGCCGTTTGGAGTGGTTGTATCGTGTAATAATTATAGTATTGGTGGGCAATTTAGTGATTGTATTTGGGTTTTGA

Protein sequence

MSRDMPQKLSKIAMDNFEESIRGDNSEIKNPSYCHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVSEHSKEVIIKLRDEVGQLKKIAQNLRRLEWLYRVIIIVLVGNLVIVFGF

Homology

[Close]

BLAST of Spg025033.1 vs. NCBI nr
Match: KAG6514840.1 (hypothetical protein ZIOFF_025214 [Zingiber officinale])

HSP 1 Score: 85.1 bits (209), Expect = 4.7e-13
Identity = 41/72 (56.94%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 34 CHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVSEHSKEVI------- 93
          CHCGLRA L TSWT+ NPGRRF+ CP Y+    GCGFF W DPE+SE SK +I       
Sbjct: 28 CHCGLRATLQTSWTESNPGRRFFSCPKYR--QKGCGFFLWHDPELSERSKTIINELKMIN 87

Query: 94 IKLRDEVGQLKK 99
           KL+ E+ +LKK
Sbjct: 88 KKLKLEINELKK 97

[Close]

BLAST of Spg025033.1 vs. NCBI nr
Match: CAD1823597.1 (unnamed protein product [Ananas comosus var. bracteatus])

HSP 1 Score: 83.6 bits (205), Expect = 1.4e-12
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 34 CHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVSEHSKEVIIKLRDEV 93
          C CGL+A   TS T  NPGRRFYGC  YK N  GCGFF W DPE+ E  KE II+L +++
Sbjct: 23 CFCGLKAPQRTSLTFDNPGRRFYGCINYKRN--GCGFFRWRDPEICERGKEYIIELLEQI 82

Query: 94 GQLKK 99
          G+LKK
Sbjct: 83 GRLKK 85

[Close]

BLAST of Spg025033.1 vs. NCBI nr
Match: KAG6514370.1 (hypothetical protein ZIOFF_024723 [Zingiber officinale])

HSP 1 Score: 82.4 bits (202), Expect = 3.1e-12
Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 0


Query: 34  CHCGLRARLITSWTKRNPGRRFYGCPGYKGN----GSGCGFFDWCDPEVSEHSKEVI--- 93
           CHCGLRA L TSWT+ NPGR F+ CP Y+ +     +GCGFF W DPE+SE SK +I   
Sbjct: 28  CHCGLRATLQTSWTESNPGRHFFSCPKYRVSFCYINTGCGFFLWHDPELSERSKTIINEL 87

Query: 94  ----IKLRDEVGQLKK 99
                KL+ E+ +LKK
Sbjct: 88  KIINKKLKLEINELKK 103

[Close]

BLAST of Spg025033.1 vs. NCBI nr
Match: KAG6473655.1 (hypothetical protein ZIOFF_067572 [Zingiber officinale])

HSP 1 Score: 81.3 bits (199), Expect = 6.8e-12
Identity = 40/72 (55.56%), Postives = 47/72 (65.28%), Query Frame = 0


Query: 34 CHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVSEHSKEVI------- 93
          C+CGLRA L  SWT+ NPGRRF+ CP YK    GCGFF W DPE SE SK +I       
Sbjct: 28 CNCGLRAILQISWTESNPGRRFFSCPKYKQR--GCGFFSWYDPEPSERSKTIINDLKKNN 87

Query: 94 IKLRDEVGQLKK 99
           KL+ E+ +LKK
Sbjct: 88 NKLKLEISELKK 97

[Close]

BLAST of Spg025033.1 vs. NCBI nr
Match: KAG6527582.1 (hypothetical protein ZIOFF_009697 [Zingiber officinale])

HSP 1 Score: 80.9 bits (198), Expect = 8.9e-12
Identity = 40/72 (55.56%), Postives = 48/72 (66.67%), Query Frame = 0


Query: 34 CHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVSEHSKEVI------- 93
          C+CGLRA L TSWT+ NP RRF+ CP Y+    GCGFF W DPEVSE SK +I       
Sbjct: 28 CYCGLRATLQTSWTESNPRRRFFSCPKYR--QKGCGFFLWHDPEVSERSKTIINELKMNN 87

Query: 94 IKLRDEVGQLKK 99
           KL+ E+ +LKK
Sbjct: 88 KKLKLEINELKK 97

[Close]

BLAST of Spg025033.1 vs. ExPASy TrEMBL
Match: A0A6V7NYI1 (GRF-type domain-containing protein OS=Ananas comosus var. bracteatus OX=296719 GN=CB5_LOCUS6808 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.6e-13
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 34 CHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVSEHSKEVIIKLRDEV 93
          C CGL+A   TS T  NPGRRFYGC  YK N  GCGFF W DPE+ E  KE II+L +++
Sbjct: 23 CFCGLKAPQRTSLTFDNPGRRFYGCINYKRN--GCGFFRWRDPEICERGKEYIIELLEQI 82

Query: 94 GQLKK 99
          G+LKK
Sbjct: 83 GRLKK 85

[Close]

BLAST of Spg025033.1 vs. ExPASy TrEMBL
Match: A0A6V7P503 (GRF-type domain-containing protein OS=Ananas comosus var. bracteatus OX=296719 GN=CB5_LOCUS9126 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 5.6e-12
Identity = 40/71 (56.34%), Postives = 48/71 (67.61%), Query Frame = 0


Query: 28 IKNPSYCHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVSEHSKEVII 87
          + NP  C CGL+A   TS T  N GRRFYGC  YK N  GCGFF W DPE+ E  KE II
Sbjct: 20 VDNP-ICFCGLKAPQRTSLTFDNLGRRFYGCINYKRN--GCGFFRWRDPEICERGKEYII 79

Query: 88 KLRDEVGQLKK 99
          +L +++G+LKK
Sbjct: 80 ELLEQIGRLKK 87

[Close]

BLAST of Spg025033.1 vs. ExPASy TrEMBL
Match: A0A445HAE1 (GRF-type domain-containing protein OS=Glycine soja OX=3848 GN=D0Y65_035499 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 7.3e-12
Identity = 42/114 (36.84%), Postives = 66/114 (57.89%), Query Frame = 0


Query: 18  EESIRGDNSEIKNPSYCHCGLRARLITSWTKRNPGRRFYGCPGYKGNGS-GCGFFDWCDP 77
           E S     S+ ++  +C C + A L+TSWT+ NPGRRFYGC  YK  G+ GC FF W DP
Sbjct: 2   ERSSSSYTSKARSRVFCLCNIEAPLVTSWTEENPGRRFYGCGLYKDRGTKGCNFFQWHDP 61

Query: 78  EVSEHSKEVIIKLRDEVGQLKKIAQNLRRL--------EWLYRVIIIVLVGNLV 123
             +   K++I+ L  EV +LK   ++L+ +        + L+ V+++  + NL+
Sbjct: 62  VDNNRQKKIIVGLMKEVDELKLREKDLQTMISEMKMKEKCLWIVLVVCWLKNLM 115

[Close]

BLAST of Spg025033.1 vs. ExPASy TrEMBL
Match: A0A445ILI1 (GRF-type domain-containing protein OS=Glycine soja OX=3848 GN=D0Y65_026829 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 9.6e-12
Identity = 38/90 (42.22%), Postives = 54/90 (60.00%), Query Frame = 0


Query: 18  EESIRGDNSEIKNPSYCHCGLRARLITSWTKRNPGRRFYGCPGYKGNGS-GCGFFDWCDP 77
           E S      E ++  +C C + A L+TSWT+ NPGRRFYGC  YK  G+ GC FF W DP
Sbjct: 88  ERSSSSYTFEARSRVFCLCNIEAPLVTSWTEENPGRRFYGCGLYKDIGTKGCNFFQWHDP 147

Query: 78  EVSEHSKEVIIKLRDEVGQLKKIAQNLRRL 107
             +   K++I+ L  EV +LK   ++L+ +
Sbjct: 148 VGNNRQKKIIVGLMKEVDELKLREKDLQTM 177

[Close]

BLAST of Spg025033.1 vs. ExPASy TrEMBL
Match: A0A0B2QQH9 (GRF-type domain-containing protein (Fragment) OS=Glycine soja OX=3848 GN=glysoja_046783 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.3e-11
Identity = 35/73 (47.95%), Postives = 46/73 (63.01%), Query Frame = 0


Query: 26 SEIKNPSYCHCGLRARLITSWTKRNPGRRFYGCPGYKGNG-SGCGFFDWCDPEVSEHSKE 85
          SE ++  +C C + A L+TSWT+ NPGRRFYGC  YK  G   C FF W DP  +   K+
Sbjct: 7  SEARSRVFCPCNIEAPLVTSWTEENPGRRFYGCGLYKDTGRKWCNFFQWLDPIGNNRQKK 66

Query: 86 VIIKLRDEVGQLK 98
          +I+ L  EV +LK
Sbjct: 67 IIVGLMKEVDELK 79

[Close]

BLAST of Spg025033.1 vs. TAIR 10
Match: AT3G42860.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 55.8 bits (133), Expect = 2.9e-08
Identity = 26/43 (60.47%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 34  CHCGLRARLI-TSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCD 76
           C CG+   LI TS T +NPGR+FY CP  + NG GCGFF WCD
Sbjct: 96  CPCGVGICLILTSNTPKNPGRKFYKCPNREENG-GCGFFQWCD 137

[Close]

BLAST of Spg025033.1 vs. TAIR 10
Match: AT1G54930.1 (GRF zinc finger / Zinc knuckle protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.9e-05
Identity = 21/49 (42.86%), Postives = 26/49 (53.06%), Query Frame = 0


Query: 30 NPSYCHCGLRA---RLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCD 76
          +P   HC   A     +TS T++NP RRFY CP        CG+F WCD
Sbjct: 34 SPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP-------SCGYFKWCD 75

[Close]

BLAST of Spg025033.1 vs. TAIR 10
Match: AT5G33393.1 (unknown protein; LOCATED IN: chloroplast; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 43.5 bits (101), Expect = 1.5e-04
Identity = 24/66 (36.36%), Postives = 32/66 (48.48%), Query Frame = 0


Query: 34 CHCGLRARLITSWTKRNPGRRFYGCPGYKGNGSGCGFFDWCDPEVS-EHSKEVIIKLRDE 93
          C+CG     I SWT  N GRRF+ C  +       GF  W D E      K  +++ RDE
Sbjct: 32 CNCGRSTTKIKSWTDDNSGRRFFRCDVH-------GFVSWSDVEKQCTWQKLSLLEARDE 90

Query: 94 VGQLKK 99
          +  LK+
Sbjct: 92 LKALKE 90

The following BLAST results are available for this feature:

BLAST of Spg025033.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6514840.1	4.7e-13	56.94	hypothetical protein ZIOFF_025214 [Zingiber officinale]	[more]
CAD1823597.1	1.4e-12	60.00	unnamed protein product [Ananas comosus var. bracteatus]	[more]
KAG6514370.1	3.1e-12	52.63	hypothetical protein ZIOFF_024723 [Zingiber officinale]	[more]
KAG6473655.1	6.8e-12	55.56	hypothetical protein ZIOFF_067572 [Zingiber officinale]	[more]
KAG6527582.1	8.9e-12	55.56	hypothetical protein ZIOFF_009697 [Zingiber officinale]	[more]

BLAST of Spg025033.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg025033.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6V7NYI1	6.6e-13	60.00	GRF-type domain-containing protein OS=Ananas comosus var. bracteatus OX=296719 G...	[more]
A0A6V7P503	5.6e-12	56.34	GRF-type domain-containing protein OS=Ananas comosus var. bracteatus OX=296719 G...	[more]
A0A445HAE1	7.3e-12	36.84	GRF-type domain-containing protein OS=Glycine soja OX=3848 GN=D0Y65_035499 PE=4 ...	[more]
A0A445ILI1	9.6e-12	42.22	GRF-type domain-containing protein OS=Glycine soja OX=3848 GN=D0Y65_026829 PE=4 ...	[more]
A0A0B2QQH9	1.3e-11	47.95	GRF-type domain-containing protein (Fragment) OS=Glycine soja OX=3848 GN=glysoja...	[more]

BLAST of Spg025033.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G42860.1	2.9e-08	60.47	zinc knuckle (CCHC-type) family protein	[more]
AT1G54930.1	3.9e-05	42.86	GRF zinc finger / Zinc knuckle protein	[more]
AT5G33393.1	1.5e-04	36.36	unknown protein; LOCATED IN: chloroplast; Has 30201 Blast hits to 17322 proteins...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 86..106
None	No IPR available	PANTHER	PTHR33248	ZINC ION-BINDING PROTEIN	coord: 16..102
IPR010666	Zinc finger, GRF-type	PFAM	PF06839	zf-GRF	coord: 33..75 e-value: 7.7E-11 score: 41.9

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg025033	Spg025033	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg025033.1-five_prime_utr	Spg025033.1-five_prime_utr-scaffold12:9729199..9729547	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg025033.1-cds	Spg025033.1-cds-scaffold12:9729548..9729733	CDS
Spg025033.1-cds	Spg025033.1-cds-scaffold12:9729839..9730036	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg025033.1-three_prime_utr	Spg025033.1-three_prime_utr-scaffold12:9730037..9730174	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg025033.1	Spg025033.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding