Spg022215.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg022215.1 vs. NCBI nr
Match: XP_011656291.1 (protein NRT1/ PTR FAMILY 6.2 [Cucumis sativus] >AGA60121.1 nitrate transporter NRT1.4B [Cucumis sativus] >KGN65321.1 hypothetical protein Csa_019823 [Cucumis sativus])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 549/580 (94.66%), Postives = 563/580 (97.07%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTAALA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISALIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCDGTKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAY ICSVSML AILLFLSGTK+YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDS DQASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DFE HG VSA NPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFEKHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL E KRL VAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAESKRLGVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVK+VTGNDG+GWLADN+NYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNVNYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKRKD QK+GIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. NCBI nr
Match: XP_008444009.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo] >KAA0046795.1 protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 548/580 (94.48%), Postives = 564/580 (97.24%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCDGTKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAY ICSVSML+AILLF+SGTK+YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKSMGSPVV ILQVIAAAIKKRKLELPQNVGLLYEDSSD ASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DF+ HG VSA NPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL ERKRLAVAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVK+VTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKRKD QK+GIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. NCBI nr
Match: TYK26929.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1073.9 bits (2776), Expect = 4.4e-310
Identity = 547/580 (94.31%), Postives = 564/580 (97.24%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCDGTKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAY ICSVSML+AILLF+SGTK+YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKS+GSPVV ILQVIAAAIKKRKLELPQNVGLLYEDSSD ASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSIGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DF+ HG VSA NPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL ERKRLAVAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVK+VTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKRKD QK+GIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. NCBI nr
Match: XP_038879372.1 (protein NRT1/ PTR FAMILY 6.2-like [Benincasa hispida])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.2e-307
Identity = 543/580 (93.62%), Postives = 561/580 (96.72%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPS+ASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTAALA++TKL
Sbjct: 61  MHLPSSASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCD TKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDDTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEV RSWAY ICSVSMLVAILLFLSGT+KYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVDRSWAYGICSVSMLVAILLFLSGTRKYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKS+GSPVVHILQVIAAAIKKRK+ELPQNVGLLYEDS DQASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSIGSPVVHILQVIAAAIKKRKMELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DFE HG VS  NPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFEKHGAVSVLNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL ERKRLAVA+AVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFIT+
Sbjct: 421 ILGMAAAALAERKRLAVAEAVGGDTATLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITR 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLS GFFISSFLVSVVK+VTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLSTLSFGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKR+D QKS IVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKREDFQKSSIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. NCBI nr
Match: KAG6589853.1 (Protein NRT1/ PTR FAMILY 6.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.4e-306
Identity = 536/579 (92.57%), Postives = 559/579 (96.55%), Query Frame = 0

Query: 1   MDGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVM 60
           MDGKLSWTVADAVDYKGFPA+KSKTGGW+PAALILGIEICERLSTMGIAVNLVTYLGGVM
Sbjct: 1   MDGKLSWTVADAVDYKGFPAEKSKTGGWVPAALILGIEICERLSTMGIAVNLVTYLGGVM 60

Query: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120
           HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALA++TKLP
Sbjct: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAVSTKLP 120

Query: 121 QLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAK 180
           QLRPPPCDG K CKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFDE DEKEKAK
Sbjct: 121 QLRPPPCDGIKDCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDETDEKEKAK 180

Query: 181 MAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYK 240
           MA+FFNRFF FISTGTLMAVTVLVYIQDEVGRSWAY ICSVSMLVAI LFLSGTK+YRYK
Sbjct: 181 MAFFFNRFFLFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAISLFLSGTKRYRYK 240

Query: 241 KSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVAE 300
           KS GSPVVHILQVIAAAIKKRKLELPQNVG+LYEDSS+Q SRIEHT+QFQFLDKAAIV +
Sbjct: 241 KSKGSPVVHILQVIAAAIKKRKLELPQNVGMLYEDSSEQGSRIEHTKQFQFLDKAAIVEQ 300

Query: 301 RDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQASTM 360
            DFE HG  SA NPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QASTM
Sbjct: 301 GDFEAHGAASALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTM 360

Query: 361 ERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLSI 420
           ER+IGSFQIPAGSLTVFFVAAILITLG+YDR+IMP+WKKWKGKQGFTNIQRMAIGLVLSI
Sbjct: 361 ERSIGSFQIPAGSLTVFFVAAILITLGVYDRIIMPLWKKWKGKQGFTNIQRMAIGLVLSI 420

Query: 421 LGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITKS 480
           LGMAAAA+ ERKRLAVAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITKS
Sbjct: 421 LGMAAAAIAERKRLAVAKAVGGDTATLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITKS 480

Query: 481 PKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLTI 540
           PKGMKTMSTGLFLTTLSLGFFISSFLVSVVK+VTG DG+GWLADNINYGRLDCFYGLLTI
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGTDGEGWLADNINYGRLDCFYGLLTI 540

Query: 541 LSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           LS+INFVAFLVCVIWY+PQKRKD QKSGIVNGN+AEE+C
Sbjct: 541 LSLINFVAFLVCVIWYVPQKRKDQQKSGIVNGNMAEERC 579

BLAST of Spg022215.1 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.5e-239
Identity = 433/565 (76.64%), Postives = 482/565 (85.31%), Query Frame = 0

Query: 1   MDGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVM 60
           M+ K SWTVADAVDYKG PADKSKTGGWI AALILGIE+ ERLSTMGIAVNLVTYL   M
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120
           HLPS+ SANIVTDFMGT FLLCLLGGFLADSFLGR+ TI I ++IQ LGT ALA+ TKLP
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 121 QLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAK 180
           +LRPP C   + C  A  FQM ILY++LYLIALGTGGLKSSISGFG+DQFD+KD KEKA 
Sbjct: 121 ELRPPTCHHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAH 180

Query: 181 MAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYK 240
           MA+FFNRFFFFIS GTL+AVTVLVY+QDEVGRSWAY IC+VSM +AI++FL GTK+YRYK
Sbjct: 181 MAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYK 240

Query: 241 KSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVAE 300
           KS GSPVV I QVIAAA +KRK+ELPQ++  LYED + +  RIEHT QF  LDKAAIVAE
Sbjct: 241 KSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYED-NPEGIRIEHTDQFHLLDKAAIVAE 300

Query: 301 RDFEMH-GTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
            DFE     V+ PNPWKLSSVT+VEEVKMM RLLPIWATTIIFWTTYAQMITFSVEQAST
Sbjct: 301 GDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           M R IGSF+IPAGSLTVFFVAAILITL +YDR IMP WKKWKGK GF+++QR+AIGLVLS
Sbjct: 361 MRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
             GMAAAALVE+KRL+VAK+      TLPISVFLL+PQFF VGAGEAFIYTGQLDFFIT+
Sbjct: 421 TAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQ 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQ-GWLADNINYGRLDCFYGLL 540
           SPKGMKTMSTGLFLTTLSLGFF+SSFLVS+VK+VT      GWLADNIN+GRLD FY LL
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLL 540

Query: 541 TILSIINFVAFLVCVIWYMPQKRKD 564
            ILS INFV +++C +W+ P K KD
Sbjct: 541 VILSGINFVVYIICALWFKPTKGKD 562

BLAST of Spg022215.1 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 8.1e-164
Identity = 307/570 (53.86%), Postives = 407/570 (71.40%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 70
           DA D++G PAD+SKTGGW  AA+IL IE  ERL+T+GI VNLVTYL G MHL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 71  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLPQLRPPPCDGT 130
           VT+F+GT F+LCLLGGF+AD+FLGRY TI I A+IQ  G + L ++T +P LRPP C+ T
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 131 KT--CKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAKMAYFFNRF 190
            +  C++A+  Q+ +LYLALYL ALGTGG+K+S+SGFG+DQFDE + KE++KM YFFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 191 FFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYKKSMGSPVV 250
           FF I+ G+L+AVTVLVY+QD+VGR W Y IC+ ++++A+ +FL+GT +YR+KK +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 251 HILQVIAAAIKKRKLELPQNVGLLY--------EDSSDQASRIEHTRQFQFLDKAAIVAE 310
            +  VI AA + RKLELP +   LY        E S     ++ HT QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI--- 313

Query: 311 RDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQASTM 370
           RD E   T +  N W LS++T VEEVK + R+LPIWAT I+FWT +AQ+ T SV Q+ T+
Sbjct: 314 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 373

Query: 371 ERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKK-WKGKQGFTNIQRMAIGLVLS 430
           +R+IGSF+IP  S+ VF+V  +L+T  +YDR+ + + KK +    G   +QR+ +GL   
Sbjct: 374 DRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFG 433

Query: 431 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 490
            + MA AALVE KRL  A A G    TLP+  +LLIPQ+  VG GEA IYTGQLDFF+ +
Sbjct: 434 SMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRE 493

Query: 491 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 550
            PKGMK MSTGL L+TL+LGFF SS LV++V+K TG     W+AD++N GRL  FY L+ 
Sbjct: 494 CPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK-AHPWIADDLNKGRLYNFYWLVA 553

Query: 551 ILSIINFVAFLVCVIWYMPQKRKDLQKSGI 570
           +L  +NF+ FLV   WY+  K K L + GI
Sbjct: 554 VLVALNFLIFLVFSKWYV-YKEKRLAEVGI 578

BLAST of Spg022215.1 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.5e-144
Identity = 278/548 (50.73%), Postives = 374/548 (68.25%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 70
           +A DY+G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL G +H+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 71  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLPQLRPPPCDGT 130
           VT+FMGT  LL LLGGFLAD+ LGRY  + ISAS+  LG   L + T +  +RPP CD  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 131 K----TCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAKMAYFFN 190
           +     C EAN  Q+ +LY+ALY IALG GG+KS++SGFG+DQFD  D KE+ +M +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 191 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYKKSMGSP 250
           RF+F IS G+L AV  LVY+QD VGR W Y I + +M+VA ++ L GTK+YR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 251 VVHILQVIAAAIKKRKLELPQNVGLL--YEDSSDQASRIEHTRQFQFLDKAAIVAERDFE 310
              I +V   A KKRK   P +  LL  Y++++     + HT   + LDKAAI       
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTT-----VPHTEMLKCLDKAAISKNESSP 315

Query: 311 MHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQASTMERTI 370
                   +PW +S+VT+VEEVK++ +L+PIWAT I+FWT Y+QM TF+VEQA+ M+R +
Sbjct: 316 SSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKL 375

Query: 371 GSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKK-WKGKQGFTNIQRMAIGLVLSILGM 430
           GSF +PAGS + F +  IL+   L +R+ +P+ ++  K  QG T++QR+ +GLV S+  M
Sbjct: 376 GSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAM 435

Query: 431 AAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITKSPKG 490
           A AA++E  R     AV  D     IS F L+PQ+F VGAGEAF Y GQL+FFI ++P+ 
Sbjct: 436 AVAAVIENARREA--AVNNDK---KISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPER 495

Query: 491 MKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLTILSI 550
           MK+MSTGLFL+T+S+GFF+SS LVS+V +VT    + WL  N+N  RL+ FY LL +L  
Sbjct: 496 MKSMSTGLFLSTISMGFFVSSLLVSLVDRVT---DKSWLRSNLNKARLNYFYWLLVVLGA 550

Query: 551 INFVAFLV 552
           +NF+ F+V
Sbjct: 556 LNFLIFIV 550

BLAST of Spg022215.1 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.7e-119
Identity = 235/559 (42.04%), Postives = 357/559 (63.86%), Query Frame = 0

Query: 1   MDGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVM 60
           M+ K  +T    VD    PA+K KTG W     ILG E CERL+  G+  NLV YL   +
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120
           +  +A +AN VT++ GTC++  L+G F+AD++LGRY+TI     I   G   L ++  +P
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 121 QLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAK 180
            L+P  C+   TC   N  Q  + ++ALY+IALGTGG+K  +S FG DQFDE DE EK K
Sbjct: 121 GLKPGNCN-ADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIK 180

Query: 181 MAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYK 240
            + FFN F+F I+ G L+A TVLV+IQ  VG  W + + +V+M++A+  F  G++ YR +
Sbjct: 181 KSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQ 240

Query: 241 KSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQAS-----RIEHTRQFQFLDKA 300
           +  GSP+  I QVI AA +K  +++P++  LL+E + D+++     ++ HT   +F DKA
Sbjct: 241 RPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKA 300

Query: 301 AIVAERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVE 360
           A+ ++ D    G V   NPW+L SVT+VEE+K +  LLP+WAT I+F T Y+QM T  V 
Sbjct: 301 AVESQSDSIKDGEV---NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVL 360

Query: 361 QASTMERTIG-SFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKW-KGKQGFTNIQRMA 420
           Q +TM++ +G +F+IP+ SL++F   ++L    +YD+ I+P+ +K+ + ++GFT +QRM 
Sbjct: 361 QGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMG 420

Query: 421 IGLVLSILGMAAAALVERKRLAVAKAVGG-DTVTLPISVFLLIPQFFFVGAGEAFIYTGQ 480
           IGLV+SI  M  A ++E  RL   K     D   + +S+F  IPQ+  +G  E F + GQ
Sbjct: 421 IGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQ 480

Query: 481 LDFFITKSPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQ-GWLADNINYGRL 540
           L+FF  ++P  M+++ + L LTT++LG ++S+ LV+VV K+T  +G+ GW+ DN+N G L
Sbjct: 481 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHL 540

Query: 541 DCFYGLLTILSIINFVAFL 551
           D F+ LL  LS +NF+ +L
Sbjct: 541 DYFFYLLATLSFLNFLVYL 554

BLAST of Spg022215.1 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 5.7e-117
Identity = 234/568 (41.20%), Postives = 364/568 (64.08%), Query Frame = 0

Query: 2   DGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMH 61
           D K  +T    +D    PA+K+KTG W     ILG E CERL+  G++ NL+ YL   M+
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMN 62

Query: 62  LPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLPQ 121
           + + +++  V+++ GTC+   L+G F+AD++LGRY+TI     I   G   L I+  +P 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 122 LRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAKM 181
           L  P C G +TC  A   Q  I ++ALYLIALGTGG+K  +S FG DQFD+ DEKEK   
Sbjct: 123 L-TPTCSG-ETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 182

Query: 182 AYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYKK 241
           + FFN F+F I+ G ++A +VLV+IQ  VG  W   + +V+M +A++ F +G+  YR +K
Sbjct: 183 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 242

Query: 242 SMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQAS-----RIEHTRQFQFLDKAA 301
             GSP+  +LQVI A+ +K K+++P++  LLYE+   ++S     ++EHT+   F DKAA
Sbjct: 243 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAA 302

Query: 302 IVAERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQ 361
           +  E D   +   +  + WKL +VT+VEE+K + RLLPIWAT I+F + Y+QM T  V Q
Sbjct: 303 VETESD---NKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQ 362

Query: 362 ASTMERTIG-SFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKG-KQGFTNIQRMAI 421
            +T+++ +G +F+IP+ SL++F   ++L    +YD+LI+P  +K+ G ++GFT +QR+ I
Sbjct: 363 GNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGI 422

Query: 422 GLVLSILGMAAAALVERKRLAVAKAVG-GDTVTLPISVFLLIPQFFFVGAGEAFIYTGQL 481
           GLV+SI  M +A ++E  RL   +     +  T+P+++F  +PQ+F VG  E F + GQL
Sbjct: 423 GLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQL 482

Query: 482 DFFITKSPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQ-GWLADNINYGRLD 541
           +FF  ++P  M+++ + L LT ++ G ++S+FLV++V KVT + G+ GW+A N+N G LD
Sbjct: 483 EFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLD 542

Query: 542 CFYGLLTILSIINFVAFLVCVIWYMPQK 561
            F+ LL  LS +NF+ +L    WY  +K
Sbjct: 543 YFFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Spg022215.1 vs. ExPASy TrEMBL
Match: L0EJZ7 (Nitrate transporter NRT1.4B OS=Cucumis sativus OX=3659 GN=Csa_1G324370 PE=2 SV=1)

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 549/580 (94.66%), Postives = 563/580 (97.07%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTAALA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISALIQTLGTAALAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCDGTKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAY ICSVSML AILLFLSGTK+YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLGAILLFLSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDS DQASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSPDQASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DFE HG VSA NPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFEKHGAVSALNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL E KRL VAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAESKRLGVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLVSVVK+VTGNDG+GWLADN+NYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVSVVKRVTGNDGEGWLADNVNYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKRKD QK+GIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. ExPASy TrEMBL
Match: A0A5A7TZJ0 (Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00850 PE=3 SV=1)

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 548/580 (94.48%), Postives = 564/580 (97.24%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCDGTKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAY ICSVSML+AILLF+SGTK+YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKSMGSPVV ILQVIAAAIKKRKLELPQNVGLLYEDSSD ASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DF+ HG VSA NPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL ERKRLAVAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVK+VTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKRKD QK+GIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. ExPASy TrEMBL
Match: A0A1S3B9G1 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo OX=3656 GN=LOC103487457 PE=3 SV=1)

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 548/580 (94.48%), Postives = 564/580 (97.24%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCDGTKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAY ICSVSML+AILLF+SGTK+YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKSMGSPVV ILQVIAAAIKKRKLELPQNVGLLYEDSSD ASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DF+ HG VSA NPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL ERKRLAVAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVK+VTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKRKD QK+GIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. ExPASy TrEMBL
Match: A0A5D3DTZ7 (Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00110 PE=3 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.2e-310
Identity = 547/580 (94.31%), Postives = 564/580 (97.24%), Query Frame = 0

Query: 1   MDG-KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60
           MDG KLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKL 120
           MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKA 180
           PQLRPPPCDGTKTCKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFD+ DEKEKA
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRY 240
           KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAY ICSVSML+AILLF+SGTK+YRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVA 300
           KKS+GSPVV ILQVIAAAIKKRKLELPQNVGLLYEDSSD ASRIEHT+QFQFLDKAAIVA
Sbjct: 241 KKSIGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 ERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
           E DF+ HG VSA NPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLG+YDRLIMP+WKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
           ILGMAAAAL ERKRLAVAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVK+VTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           ILSIINFVAFLVCVIWYMPQKRKD QK+GIVNGNLAEEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Spg022215.1 vs. ExPASy TrEMBL
Match: A0A6J1JHI8 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita maxima OX=3661 GN=LOC111485151 PE=3 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 5.8e-306
Identity = 535/579 (92.40%), Postives = 558/579 (96.37%), Query Frame = 0

Query: 1   MDGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVM 60
           MDGKLSWTVADAVDYKGFPA+KSKTGGW+PAALILGIEICERLSTMGIAVNLVTYLGGVM
Sbjct: 1   MDGKLSWTVADAVDYKGFPAEKSKTGGWVPAALILGIEICERLSTMGIAVNLVTYLGGVM 60

Query: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120
           HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALA++TKLP
Sbjct: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAVSTKLP 120

Query: 121 QLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAK 180
           QLRPPPCDG K CKEAN FQMGILYLALYLIALGTGGLKSSISGFGTDQFDE DEKEKAK
Sbjct: 121 QLRPPPCDGIKDCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDETDEKEKAK 180

Query: 181 MAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYK 240
           MA+FFNRFF FISTGTLMAVTVLVYIQDEVGRSWAY ICSVSMLVAI LFLSGTK+YRYK
Sbjct: 181 MAFFFNRFFLFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAISLFLSGTKRYRYK 240

Query: 241 KSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVAE 300
           KS GSPVVHILQVIAAAIKKRKLELPQNVG+LYEDSS+Q SRIEHT+QFQFLDKAAIV +
Sbjct: 241 KSKGSPVVHILQVIAAAIKKRKLELPQNVGMLYEDSSEQGSRIEHTKQFQFLDKAAIVEQ 300

Query: 301 RDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQASTM 360
            DFE HG  SA NPWKLSSVT+VEEVKMMARLLPIWATTIIFWTTYAQMITFSV+QASTM
Sbjct: 301 GDFEAHGAASALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQASTM 360

Query: 361 ERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLSI 420
           ER+IGSFQIPAGSLTVFFVAAILITLG+YDR+IMP+WKKWKGKQGFTNIQRMAIGLVLSI
Sbjct: 361 ERSIGSFQIPAGSLTVFFVAAILITLGVYDRIIMPLWKKWKGKQGFTNIQRMAIGLVLSI 420

Query: 421 LGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITKS 480
           LGMAAAA+ ERKRLAVAKAVGGDT TLPISVFLLIPQFF VGAGEAFIYTGQLDFFITK+
Sbjct: 421 LGMAAAAIAERKRLAVAKAVGGDTATLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITKA 480

Query: 481 PKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLTI 540
           PKGMKTMSTGLFLTTLSLGFFISSFLVSVVK+VTG DG+GWLADNINYGRLDCFYGLLTI
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGTDGEGWLADNINYGRLDCFYGLLTI 540

Query: 541 LSIINFVAFLVCVIWYMPQKRKDLQKSGIVNGNLAEEKC 580
           LS+INFVAFLVCVIWY+PQKRKD QKSGIVN N+AEEKC
Sbjct: 541 LSLINFVAFLVCVIWYVPQKRKDQQKSGIVNSNMAEEKC 579

BLAST of Spg022215.1 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 830.1 bits (2143), Expect = 1.1e-240
Identity = 433/565 (76.64%), Postives = 482/565 (85.31%), Query Frame = 0

Query: 1   MDGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVM 60
           M+ K SWTVADAVDYKG PADKSKTGGWI AALILGIE+ ERLSTMGIAVNLVTYL   M
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120
           HLPS+ SANIVTDFMGT FLLCLLGGFLADSFLGR+ TI I ++IQ LGT ALA+ TKLP
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 121 QLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAK 180
           +LRPP C   + C  A  FQM ILY++LYLIALGTGGLKSSISGFG+DQFD+KD KEKA 
Sbjct: 121 ELRPPTCHHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAH 180

Query: 181 MAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYK 240
           MA+FFNRFFFFIS GTL+AVTVLVY+QDEVGRSWAY IC+VSM +AI++FL GTK+YRYK
Sbjct: 181 MAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYK 240

Query: 241 KSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQASRIEHTRQFQFLDKAAIVAE 300
           KS GSPVV I QVIAAA +KRK+ELPQ++  LYED + +  RIEHT QF  LDKAAIVAE
Sbjct: 241 KSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYED-NPEGIRIEHTDQFHLLDKAAIVAE 300

Query: 301 RDFEMH-GTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQAST 360
            DFE     V+ PNPWKLSSVT+VEEVKMM RLLPIWATTIIFWTTYAQMITFSVEQAST
Sbjct: 301 GDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKGKQGFTNIQRMAIGLVLS 420
           M R IGSF+IPAGSLTVFFVAAILITL +YDR IMP WKKWKGK GF+++QR+AIGLVLS
Sbjct: 361 MRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLS 420

Query: 421 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 480
             GMAAAALVE+KRL+VAK+      TLPISVFLL+PQFF VGAGEAFIYTGQLDFFIT+
Sbjct: 421 TAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQ 480

Query: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQ-GWLADNINYGRLDCFYGLL 540
           SPKGMKTMSTGLFLTTLSLGFF+SSFLVS+VK+VT      GWLADNIN+GRLD FY LL
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLL 540

Query: 541 TILSIINFVAFLVCVIWYMPQKRKD 564
            ILS INFV +++C +W+ P K KD
Sbjct: 541 VILSGINFVVYIICALWFKPTKGKD 562

BLAST of Spg022215.1 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 578.6 bits (1490), Expect = 5.8e-165
Identity = 307/570 (53.86%), Postives = 407/570 (71.40%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 70
           DA D++G PAD+SKTGGW  AA+IL IE  ERL+T+GI VNLVTYL G MHL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 71  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLPQLRPPPCDGT 130
           VT+F+GT F+LCLLGGF+AD+FLGRY TI I A+IQ  G + L ++T +P LRPP C+ T
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 131 KT--CKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAKMAYFFNRF 190
            +  C++A+  Q+ +LYLALYL ALGTGG+K+S+SGFG+DQFDE + KE++KM YFFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 191 FFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYKKSMGSPVV 250
           FF I+ G+L+AVTVLVY+QD+VGR W Y IC+ ++++A+ +FL+GT +YR+KK +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 251 HILQVIAAAIKKRKLELPQNVGLLY--------EDSSDQASRIEHTRQFQFLDKAAIVAE 310
            +  VI AA + RKLELP +   LY        E S     ++ HT QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI--- 313

Query: 311 RDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQASTM 370
           RD E   T +  N W LS++T VEEVK + R+LPIWAT I+FWT +AQ+ T SV Q+ T+
Sbjct: 314 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 373

Query: 371 ERTIGSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKK-WKGKQGFTNIQRMAIGLVLS 430
           +R+IGSF+IP  S+ VF+V  +L+T  +YDR+ + + KK +    G   +QR+ +GL   
Sbjct: 374 DRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFG 433

Query: 431 ILGMAAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITK 490
            + MA AALVE KRL  A A G    TLP+  +LLIPQ+  VG GEA IYTGQLDFF+ +
Sbjct: 434 SMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRE 493

Query: 491 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLT 550
            PKGMK MSTGL L+TL+LGFF SS LV++V+K TG     W+AD++N GRL  FY L+ 
Sbjct: 494 CPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK-AHPWIADDLNKGRLYNFYWLVA 553

Query: 551 ILSIINFVAFLVCVIWYMPQKRKDLQKSGI 570
           +L  +NF+ FLV   WY+  K K L + GI
Sbjct: 554 VLVALNFLIFLVFSKWYV-YKEKRLAEVGI 578

BLAST of Spg022215.1 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.7e-145
Identity = 278/548 (50.73%), Postives = 374/548 (68.25%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMHLPSAASANI 70
           +A DY+G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL G +H+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 71  VTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLPQLRPPPCDGT 130
           VT+FMGT  LL LLGGFLAD+ LGRY  + ISAS+  LG   L + T +  +RPP CD  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 131 K----TCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAKMAYFFN 190
           +     C EAN  Q+ +LY+ALY IALG GG+KS++SGFG+DQFD  D KE+ +M +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 191 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYKKSMGSP 250
           RF+F IS G+L AV  LVY+QD VGR W Y I + +M+VA ++ L GTK+YR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 251 VVHILQVIAAAIKKRKLELPQNVGLL--YEDSSDQASRIEHTRQFQFLDKAAIVAERDFE 310
              I +V   A KKRK   P +  LL  Y++++     + HT   + LDKAAI       
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTT-----VPHTEMLKCLDKAAISKNESSP 315

Query: 311 MHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQASTMERTI 370
                   +PW +S+VT+VEEVK++ +L+PIWAT I+FWT Y+QM TF+VEQA+ M+R +
Sbjct: 316 SSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKL 375

Query: 371 GSFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKK-WKGKQGFTNIQRMAIGLVLSILGM 430
           GSF +PAGS + F +  IL+   L +R+ +P+ ++  K  QG T++QR+ +GLV S+  M
Sbjct: 376 GSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAM 435

Query: 431 AAAALVERKRLAVAKAVGGDTVTLPISVFLLIPQFFFVGAGEAFIYTGQLDFFITKSPKG 490
           A AA++E  R     AV  D     IS F L+PQ+F VGAGEAF Y GQL+FFI ++P+ 
Sbjct: 436 AVAAVIENARREA--AVNNDK---KISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPER 495

Query: 491 MKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQGWLADNINYGRLDCFYGLLTILSI 550
           MK+MSTGLFL+T+S+GFF+SS LVS+V +VT    + WL  N+N  RL+ FY LL +L  
Sbjct: 496 MKSMSTGLFLSTISMGFFVSSLLVSLVDRVT---DKSWLRSNLNKARLNYFYWLLVVLGA 550

Query: 551 INFVAFLV 552
           +NF+ F+V
Sbjct: 556 LNFLIFIV 550

BLAST of Spg022215.1 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 430.6 bits (1106), Expect = 1.9e-120
Identity = 235/559 (42.04%), Postives = 357/559 (63.86%), Query Frame = 0

Query: 1   MDGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVM 60
           M+ K  +T    VD    PA+K KTG W     ILG E CERL+  G+  NLV YL   +
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 61  HLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120
           +  +A +AN VT++ GTC++  L+G F+AD++LGRY+TI     I   G   L ++  +P
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 121 QLRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAK 180
            L+P  C+   TC   N  Q  + ++ALY+IALGTGG+K  +S FG DQFDE DE EK K
Sbjct: 121 GLKPGNCN-ADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIK 180

Query: 181 MAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYK 240
            + FFN F+F I+ G L+A TVLV+IQ  VG  W + + +V+M++A+  F  G++ YR +
Sbjct: 181 KSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQ 240

Query: 241 KSMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQAS-----RIEHTRQFQFLDKA 300
           +  GSP+  I QVI AA +K  +++P++  LL+E + D+++     ++ HT   +F DKA
Sbjct: 241 RPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKA 300

Query: 301 AIVAERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVE 360
           A+ ++ D    G V   NPW+L SVT+VEE+K +  LLP+WAT I+F T Y+QM T  V 
Sbjct: 301 AVESQSDSIKDGEV---NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVL 360

Query: 361 QASTMERTIG-SFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKW-KGKQGFTNIQRMA 420
           Q +TM++ +G +F+IP+ SL++F   ++L    +YD+ I+P+ +K+ + ++GFT +QRM 
Sbjct: 361 QGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMG 420

Query: 421 IGLVLSILGMAAAALVERKRLAVAKAVGG-DTVTLPISVFLLIPQFFFVGAGEAFIYTGQ 480
           IGLV+SI  M  A ++E  RL   K     D   + +S+F  IPQ+  +G  E F + GQ
Sbjct: 421 IGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQ 480

Query: 481 LDFFITKSPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQ-GWLADNINYGRL 540
           L+FF  ++P  M+++ + L LTT++LG ++S+ LV+VV K+T  +G+ GW+ DN+N G L
Sbjct: 481 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHL 540

Query: 541 DCFYGLLTILSIINFVAFL 551
           D F+ LL  LS +NF+ +L
Sbjct: 541 DYFFYLLATLSFLNFLVYL 554

BLAST of Spg022215.1 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 422.9 bits (1086), Expect = 4.0e-118
Identity = 234/568 (41.20%), Postives = 364/568 (64.08%), Query Frame = 0

Query: 2   DGKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGVMH 61
           D K  +T    +D    PA+K+KTG W     ILG E CERL+  G++ NL+ YL   M+
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMN 62

Query: 62  LPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLPQ 121
           + + +++  V+++ GTC+   L+G F+AD++LGRY+TI     I   G   L I+  +P 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 122 LRPPPCDGTKTCKEANEFQMGILYLALYLIALGTGGLKSSISGFGTDQFDEKDEKEKAKM 181
           L  P C G +TC  A   Q  I ++ALYLIALGTGG+K  +S FG DQFD+ DEKEK   
Sbjct: 123 L-TPTCSG-ETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 182

Query: 182 AYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYAICSVSMLVAILLFLSGTKKYRYKK 241
           + FFN F+F I+ G ++A +VLV+IQ  VG  W   + +V+M +A++ F +G+  YR +K
Sbjct: 183 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 242

Query: 242 SMGSPVVHILQVIAAAIKKRKLELPQNVGLLYEDSSDQAS-----RIEHTRQFQFLDKAA 301
             GSP+  +LQVI A+ +K K+++P++  LLYE+   ++S     ++EHT+   F DKAA
Sbjct: 243 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAA 302

Query: 302 IVAERDFEMHGTVSAPNPWKLSSVTRVEEVKMMARLLPIWATTIIFWTTYAQMITFSVEQ 361
           +  E D   +   +  + WKL +VT+VEE+K + RLLPIWAT I+F + Y+QM T  V Q
Sbjct: 303 VETESD---NKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQ 362

Query: 362 ASTMERTIG-SFQIPAGSLTVFFVAAILITLGLYDRLIMPIWKKWKG-KQGFTNIQRMAI 421
            +T+++ +G +F+IP+ SL++F   ++L    +YD+LI+P  +K+ G ++GFT +QR+ I
Sbjct: 363 GNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGI 422

Query: 422 GLVLSILGMAAAALVERKRLAVAKAVG-GDTVTLPISVFLLIPQFFFVGAGEAFIYTGQL 481
           GLV+SI  M +A ++E  RL   +     +  T+P+++F  +PQ+F VG  E F + GQL
Sbjct: 423 GLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQL 482

Query: 482 DFFITKSPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKKVTGNDGQ-GWLADNINYGRLD 541
           +FF  ++P  M+++ + L LT ++ G ++S+FLV++V KVT + G+ GW+A N+N G LD
Sbjct: 483 EFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLD 542

Query: 542 CFYGLLTILSIINFVAFLVCVIWYMPQK 561
            F+ LL  LS +NF+ +L    WY  +K
Sbjct: 543 YFFWLLAGLSFLNFLVYLWIAKWYTYKK 564