Spg017181.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg017181.1 vs. NCBI nr
Match: XP_038889620.1 (uncharacterized protein LOC120079490 isoform X1 [Benincasa hispida] >XP_038889621.1 uncharacterized protein LOC120079490 isoform X2 [Benincasa hispida] >XP_038889622.1 uncharacterized protein LOC120079490 isoform X3 [Benincasa hispida])

HSP 1 Score: 617.1 bits (1590), Expect = 1.5e-172
Identity = 333/409 (81.42%), Postives = 364/409 (89.00%), Query Frame = 0

Query: 145 VSEASEGVSALEQAQEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDKVVPTK 204
           + +ASEGV AL+QA+EV +V  IDLNKDS++KLEEQIELC+TTEKT  PIIEDDKVVP+K
Sbjct: 74  ILKASEGVCALKQAKEVADVSLIDLNKDSLEKLEEQIELCYTTEKTY-PIIEDDKVVPSK 133

Query: 205 EVENNLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDD-DDEYVEEIDHL 264
           E+ENNL EIKKEEKSGRE+DE+RGSSN SNAFSL LNHRYA C NSDD D+EY EE+DHL
Sbjct: 134 EIENNLDEIKKEEKSGREDDEARGSSNSSNAFSLPLNHRYAVCQNSDDYDEEYEEEMDHL 193

Query: 265 DEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDPS 324
           D E+EEERK +   DDG+ K KLL KQESSESLFSLS+ SR QV   E+GE EVNSPD S
Sbjct: 194 D-EKEEERKDEAADDDGDEKDKLLTKQESSESLFSLSLGSRIQVFTFESGENEVNSPDLS 253

Query: 325 HCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENISLD 384
           HCS DIQPDKA SDKK VCRN+N++SVLNPIENVT  LN  KV T+PPVHHLEKENI+LD
Sbjct: 254 HCSLDIQPDKALSDKKPVCRNENINSVLNPIENVTHSLNDAKVITLPPVHHLEKENINLD 313

Query: 385 KGCDVHISPEPTFRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTSSIG 444
           K CDV ISPEPTFRSMRKLKEN SD+KHVEDEIAVDTSLSNWLVESETTPKSKS++SSIG
Sbjct: 314 KDCDVSISPEPTFRSMRKLKENCSDVKHVEDEIAVDTSLSNWLVESETTPKSKSNSSSIG 373

Query: 445 NSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYWSHT 504
           NSP+WTRNSAKSYEDRPILGALTLEELRQFS FSTPRRSRSRSPEETPIIG+VGSYWSHT
Sbjct: 374 NSPMWTRNSAKSYEDRPILGALTLEELRQFSAFSTPRRSRSRSPEETPIIGSVGSYWSHT 433

Query: 505 GQDGDSNPGSSCRGLTKTRKSREDERVNWNSTPFLERLDMALASNSAEV 553
           GQD DSNPGSSCRG   TRK+REDE+VNWNSTPFLERLDMALASNSAEV
Sbjct: 434 GQDADSNPGSSCRGSKTTRKNREDEKVNWNSTPFLERLDMALASNSAEV 480

BLAST of Spg017181.1 vs. NCBI nr
Match: XP_011649011.1 (eisosome protein SEG2 [Cucumis sativus] >KGN61283.1 hypothetical protein Csa_006279 [Cucumis sativus])

HSP 1 Score: 593.6 bits (1529), Expect = 1.8e-165
Identity = 323/410 (78.78%), Postives = 364/410 (88.78%), Query Frame = 0

Query: 145 VSEASEGVSALEQA-QEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDK-VVP 204
           + +ASEGV ALEQA +EV++V  +DLN DS++KLEE+I+LC TTEK   PIIEDDK VVP
Sbjct: 30  ILKASEGVCALEQAKKEVVDVCLVDLNNDSLEKLEERIKLCDTTEKAC-PIIEDDKIVVP 89

Query: 205 TKEVENNLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEIDH 264
           T+EVE+NL +IK EEKSGRE+DESRGSSNLSNAFS+ LNHRYA C NSDDD+E+VEE+DH
Sbjct: 90  TEEVEHNLDDIKIEEKSGREDDESRGSSNLSNAFSIPLNHRYAVCQNSDDDEEFVEEMDH 149

Query: 265 LDEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDP 324
           L  EQEEERK D + DDG+G++KLLIKQESSESLFSLS+ SRKQV   EA E E+NSPDP
Sbjct: 150 LG-EQEEERKDDADNDDGDGENKLLIKQESSESLFSLSLGSRKQVFTFEADENEINSPDP 209

Query: 325 SHCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENISL 384
           SH S D+Q DKASSDK S+C+ +NVDSVL PIENVT CLNA KV T+P VHHLEKENI+L
Sbjct: 210 SHHSLDLQLDKASSDKISICQIENVDSVLKPIENVTHCLNAAKVATLPLVHHLEKENINL 269

Query: 385 DKGCDVHISPEPTFRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTSSI 444
           ++ CDV ISPEPTFRSMRKLKE+RSDLKHVEDEIAVDTSLSNWLVESETTPKSKS++SSI
Sbjct: 270 EQDCDVLISPEPTFRSMRKLKESRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSNSSSI 329

Query: 445 GNSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYWSH 504
           GNSP+WT+NSAKSYEDRPILGALTLEELRQFS  STPRR RSRSPEETPIIG+VGSYWSH
Sbjct: 330 GNSPMWTKNSAKSYEDRPILGALTLEELRQFSASSTPRRYRSRSPEETPIIGSVGSYWSH 389

Query: 505 TGQDGDSNPGSSCRGLTKTRKSREDERVNWNSTPFLERLDMALASNSAEV 553
           TGQD DSNPGSSCRG   TR++RE+ERVNWNSTPFLERLDMALASNSAEV
Sbjct: 390 TGQDADSNPGSSCRGPKTTRRNREEERVNWNSTPFLERLDMALASNSAEV 437

BLAST of Spg017181.1 vs. NCBI nr
Match: XP_008441823.1 (PREDICTED: uncharacterized protein LOC103485873 [Cucumis melo] >KAA0049973.1 protein JASON-like [Cucumis melo var. makuwa])

HSP 1 Score: 553.5 bits (1425), Expect = 2.0e-153
Identity = 307/412 (74.51%), Postives = 350/412 (84.95%), Query Frame = 0

Query: 145 VSEASEGVSALEQA-QEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDK-VVP 204
           + +ASEGV ALEQA +E++ V  +DLN  S++KLEE+I+LC TTEKT  P+IEDDK VVP
Sbjct: 31  ILKASEGVCALEQAKKEMVGVSLVDLNNGSLEKLEERIKLCDTTEKTC-PVIEDDKIVVP 90

Query: 205 TKEVEN--NLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEI 264
           TKEVE+  NL +IK EEKSGRE+DESRGSSNLSNAFS+ LNHRYA C NSDDD+E+VEE+
Sbjct: 91  TKEVEHNLNLDDIKIEEKSGREDDESRGSSNLSNAFSIPLNHRYAVCQNSDDDEEFVEEM 150

Query: 265 DHLDEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSP 324
           DHL  E+EEERK D + DDG+G++KLLIKQESSESLFSLS+ SRKQV   EA E E+NSP
Sbjct: 151 DHLG-EKEEERKDDADNDDGDGENKLLIKQESSESLFSLSLGSRKQVFTFEADENEINSP 210

Query: 325 DPSHCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENI 384
           DPSH S D+QP KASSDK S+C+ +N+DSVL PIENVT CLNA KV T+P VH LEKENI
Sbjct: 211 DPSHRSLDLQPGKASSDKISICQKENIDSVLKPIENVTHCLNAAKVATLPLVHQLEKENI 270

Query: 385 SLDKGCDVHISPEPTFRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTS 444
           +L++ CDV ISPEPTFRSMR         KHVEDEI VDTSLSNWLVESE TPKS S++S
Sbjct: 271 NLEQDCDVLISPEPTFRSMR---------KHVEDEIGVDTSLSNWLVESEITPKSNSNSS 330

Query: 445 SIGNSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYW 504
           SIGNSP+WTRNSAKS+EDRPILGALTLEELRQFS  STPRR+ SRSPEETPIIG+VGSYW
Sbjct: 331 SIGNSPMWTRNSAKSHEDRPILGALTLEELRQFSASSTPRRNISRSPEETPIIGSVGSYW 390

Query: 505 SHTGQDGDSNPGSSCRGLTKTRKSREDERVNWNSTPFLERLDMALASNSAEV 553
           SHTGQD DSNPGSSCRG   TR++RE+ERVNWNSTPFLERLDMALASNSAEV
Sbjct: 391 SHTGQDADSNPGSSCRGPKTTRRNREEERVNWNSTPFLERLDMALASNSAEV 431

BLAST of Spg017181.1 vs. NCBI nr
Match: XP_022149810.1 (uncharacterized protein LOC111018155 [Momordica charantia])

HSP 1 Score: 519.2 bits (1336), Expect = 4.2e-143
Identity = 298/412 (72.33%), Postives = 338/412 (82.04%), Query Frame = 0

Query: 145 VSEASEGVSALEQAQEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDKVVPTK 204
           + + +E V  L++A+E  ++ S+ LN DS++K+ EQIELCH+ EKTSDPI+EDD VVPT+
Sbjct: 27  ILKPNEAVHPLDRAKEEPQLSSVSLNADSLEKVGEQIELCHSGEKTSDPIVEDDAVVPTQ 86

Query: 205 EVENNLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEIDHLD 264
           +VEN L E+KKEEKSG E+DE RGSSNLS AFSL LNHRYA C NS +DDE VEE+D LD
Sbjct: 87  DVENVLDEVKKEEKSGLEDDEPRGSSNLSTAFSLPLNHRYADCRNS-NDDECVEEMDLLD 146

Query: 265 EEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDPSH 324
           E   E RK     DDG+G+S+L ++QESSESLFSLS+ SRKQ+CAVEAGE EVNSP PSH
Sbjct: 147 E--GEGRK-----DDGDGRSELFVQQESSESLFSLSLGSRKQICAVEAGENEVNSPAPSH 206

Query: 325 CSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENISLDK 384
           CS   QP+KAS  +K+ C+N N DSVL PIENVT  LNA +VKTVPPV  LEKENI  +K
Sbjct: 207 CSCGTQPEKASPGQKTGCQNRNADSVLAPIENVTH-LNAARVKTVPPV-RLEKENIDSEK 266

Query: 385 GCDVHISPEPT-FRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTSSIG 444
             DV ISPEPT  R +RKLKEN S+ K  EDEIAVDTSLSNWL+E +TTPKSKSS+SSIG
Sbjct: 267 DYDVPISPEPTPKRPLRKLKENHSNSKPAEDEIAVDTSLSNWLIECDTTPKSKSSSSSIG 326

Query: 445 NSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYWSHT 504
           NSP+WTRNSAKSYEDRPILGALTLEELRQFS FSTPRRSRSRSPEETPIIGTVGSYWSHT
Sbjct: 327 NSPMWTRNSAKSYEDRPILGALTLEELRQFSAFSTPRRSRSRSPEETPIIGTVGSYWSHT 386

Query: 505 GQDGDSNPGSS--CRGLTKT-RKSREDERVNWNSTPFLERLDMALASNSAEV 553
            QD D NPGSS  C G TKT RK+REDERVNWNSTPFLERLD ALASNSAEV
Sbjct: 387 RQDADFNPGSSSPCTGSTKTARKNREDERVNWNSTPFLERLDRALASNSAEV 428

BLAST of Spg017181.1 vs. NCBI nr
Match: XP_022965449.1 (uncharacterized protein LOC111465352 isoform X2 [Cucurbita maxima])

HSP 1 Score: 517.3 bits (1331), Expect = 1.6e-142
Identity = 319/537 (59.40%), Postives = 355/537 (66.11%), Query Frame = 0

Query: 145 VSEASEGVSALEQAQEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDKVVPTK 204
           V +AS+GV ALE+ +EV EVP IDL KDS++KLEEQIELCH TEK S+PIIED+KVVP+K
Sbjct: 30  VFKASDGVCALEKTKEVAEVPPIDLIKDSLEKLEEQIELCHATEKASEPIIEDNKVVPSK 89

Query: 205 EVENNLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEIDHLD 264
           E E+ L EIK E+KSG+EEDES GSSNLS AF+L LNHRY  CSNSDD+DEY EE+DHLD
Sbjct: 90  ENESKLDEIKDEKKSGKEEDESWGSSNLSQAFTLSLNHRYENCSNSDDNDEYAEEMDHLD 149

Query: 265 EEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDPSH 324
           E++EE+RK DTNADDG+G+S LL++QESSESLFSLS  SRKQVC VE  E EV+S D SH
Sbjct: 150 EKEEEKRKDDTNADDGHGESMLLMEQESSESLFSLSFGSRKQVCGVEDAENEVSSLDLSH 209

Query: 325 CSADI------------------------------QPDKASSDKKSVCRNDN-------- 384
              +                               QP+ ASS+KK+ C N+N        
Sbjct: 210 QQENASSEKKTGCWNKNVVGSVLKPIENDTRCLSHQPNNASSEKKTGCWNENVVGSVLNP 269

Query: 385 --------------------------------------------------VDSVLNPIEN 444
                                                             V SVLNPIEN
Sbjct: 270 IENDTRCLSHQPDNASSKKNTGRRNENVVENVTRCLSQQPENASSDKKTDVGSVLNPIEN 329

Query: 445 V-TQCLNATKVKTVPPVHHLEKENISLDKGCDV-HISPEPTFRSMRKLKENRSDLKHVED 504
           V T CL+A KVK +P  HHLEKENISLDKGCDV  ISPEPTFR+MRKLKEN SD KHVED
Sbjct: 330 VTTHCLSAAKVKALPSDHHLEKENISLDKGCDVLLISPEPTFRAMRKLKENCSD-KHVED 389

Query: 505 EIAVDTSLSNWLVESETTPKSKSSTSSIGNSPIWTRNSAKSYEDRPILGALTLEELRQFS 553
           EIAVDTSLSNWLVESETTPKS S++SSIGNSP+WTRNSAKS EDRPILGALTLEE+RQFS
Sbjct: 390 EIAVDTSLSNWLVESETTPKSNSTSSSIGNSPMWTRNSAKSNEDRPILGALTLEEIRQFS 449

BLAST of Spg017181.1 vs. ExPASy TrEMBL
Match: A0A0A0LHL1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G075420 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 8.6e-166
Identity = 323/410 (78.78%), Postives = 364/410 (88.78%), Query Frame = 0

Query: 145 VSEASEGVSALEQA-QEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDK-VVP 204
           + +ASEGV ALEQA +EV++V  +DLN DS++KLEE+I+LC TTEK   PIIEDDK VVP
Sbjct: 30  ILKASEGVCALEQAKKEVVDVCLVDLNNDSLEKLEERIKLCDTTEKAC-PIIEDDKIVVP 89

Query: 205 TKEVENNLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEIDH 264
           T+EVE+NL +IK EEKSGRE+DESRGSSNLSNAFS+ LNHRYA C NSDDD+E+VEE+DH
Sbjct: 90  TEEVEHNLDDIKIEEKSGREDDESRGSSNLSNAFSIPLNHRYAVCQNSDDDEEFVEEMDH 149

Query: 265 LDEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDP 324
           L  EQEEERK D + DDG+G++KLLIKQESSESLFSLS+ SRKQV   EA E E+NSPDP
Sbjct: 150 LG-EQEEERKDDADNDDGDGENKLLIKQESSESLFSLSLGSRKQVFTFEADENEINSPDP 209

Query: 325 SHCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENISL 384
           SH S D+Q DKASSDK S+C+ +NVDSVL PIENVT CLNA KV T+P VHHLEKENI+L
Sbjct: 210 SHHSLDLQLDKASSDKISICQIENVDSVLKPIENVTHCLNAAKVATLPLVHHLEKENINL 269

Query: 385 DKGCDVHISPEPTFRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTSSI 444
           ++ CDV ISPEPTFRSMRKLKE+RSDLKHVEDEIAVDTSLSNWLVESETTPKSKS++SSI
Sbjct: 270 EQDCDVLISPEPTFRSMRKLKESRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSNSSSI 329

Query: 445 GNSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYWSH 504
           GNSP+WT+NSAKSYEDRPILGALTLEELRQFS  STPRR RSRSPEETPIIG+VGSYWSH
Sbjct: 330 GNSPMWTKNSAKSYEDRPILGALTLEELRQFSASSTPRRYRSRSPEETPIIGSVGSYWSH 389

Query: 505 TGQDGDSNPGSSCRGLTKTRKSREDERVNWNSTPFLERLDMALASNSAEV 553
           TGQD DSNPGSSCRG   TR++RE+ERVNWNSTPFLERLDMALASNSAEV
Sbjct: 390 TGQDADSNPGSSCRGPKTTRRNREEERVNWNSTPFLERLDMALASNSAEV 437

BLAST of Spg017181.1 vs. ExPASy TrEMBL
Match: A0A5A7U262 (Protein JASON-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold13G001430 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 9.8e-154
Identity = 307/412 (74.51%), Postives = 350/412 (84.95%), Query Frame = 0

Query: 145 VSEASEGVSALEQA-QEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDK-VVP 204
           + +ASEGV ALEQA +E++ V  +DLN  S++KLEE+I+LC TTEKT  P+IEDDK VVP
Sbjct: 31  ILKASEGVCALEQAKKEMVGVSLVDLNNGSLEKLEERIKLCDTTEKTC-PVIEDDKIVVP 90

Query: 205 TKEVEN--NLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEI 264
           TKEVE+  NL +IK EEKSGRE+DESRGSSNLSNAFS+ LNHRYA C NSDDD+E+VEE+
Sbjct: 91  TKEVEHNLNLDDIKIEEKSGREDDESRGSSNLSNAFSIPLNHRYAVCQNSDDDEEFVEEM 150

Query: 265 DHLDEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSP 324
           DHL  E+EEERK D + DDG+G++KLLIKQESSESLFSLS+ SRKQV   EA E E+NSP
Sbjct: 151 DHLG-EKEEERKDDADNDDGDGENKLLIKQESSESLFSLSLGSRKQVFTFEADENEINSP 210

Query: 325 DPSHCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENI 384
           DPSH S D+QP KASSDK S+C+ +N+DSVL PIENVT CLNA KV T+P VH LEKENI
Sbjct: 211 DPSHRSLDLQPGKASSDKISICQKENIDSVLKPIENVTHCLNAAKVATLPLVHQLEKENI 270

Query: 385 SLDKGCDVHISPEPTFRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTS 444
           +L++ CDV ISPEPTFRSMR         KHVEDEI VDTSLSNWLVESE TPKS S++S
Sbjct: 271 NLEQDCDVLISPEPTFRSMR---------KHVEDEIGVDTSLSNWLVESEITPKSNSNSS 330

Query: 445 SIGNSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYW 504
           SIGNSP+WTRNSAKS+EDRPILGALTLEELRQFS  STPRR+ SRSPEETPIIG+VGSYW
Sbjct: 331 SIGNSPMWTRNSAKSHEDRPILGALTLEELRQFSASSTPRRNISRSPEETPIIGSVGSYW 390

Query: 505 SHTGQDGDSNPGSSCRGLTKTRKSREDERVNWNSTPFLERLDMALASNSAEV 553
           SHTGQD DSNPGSSCRG   TR++RE+ERVNWNSTPFLERLDMALASNSAEV
Sbjct: 391 SHTGQDADSNPGSSCRGPKTTRRNREEERVNWNSTPFLERLDMALASNSAEV 431

BLAST of Spg017181.1 vs. ExPASy TrEMBL
Match: A0A1S3B4Z2 (uncharacterized protein LOC103485873 OS=Cucumis melo OX=3656 GN=LOC103485873 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 9.8e-154
Identity = 307/412 (74.51%), Postives = 350/412 (84.95%), Query Frame = 0

Query: 145 VSEASEGVSALEQA-QEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDK-VVP 204
           + +ASEGV ALEQA +E++ V  +DLN  S++KLEE+I+LC TTEKT  P+IEDDK VVP
Sbjct: 31  ILKASEGVCALEQAKKEMVGVSLVDLNNGSLEKLEERIKLCDTTEKTC-PVIEDDKIVVP 90

Query: 205 TKEVEN--NLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEI 264
           TKEVE+  NL +IK EEKSGRE+DESRGSSNLSNAFS+ LNHRYA C NSDDD+E+VEE+
Sbjct: 91  TKEVEHNLNLDDIKIEEKSGREDDESRGSSNLSNAFSIPLNHRYAVCQNSDDDEEFVEEM 150

Query: 265 DHLDEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSP 324
           DHL  E+EEERK D + DDG+G++KLLIKQESSESLFSLS+ SRKQV   EA E E+NSP
Sbjct: 151 DHLG-EKEEERKDDADNDDGDGENKLLIKQESSESLFSLSLGSRKQVFTFEADENEINSP 210

Query: 325 DPSHCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENI 384
           DPSH S D+QP KASSDK S+C+ +N+DSVL PIENVT CLNA KV T+P VH LEKENI
Sbjct: 211 DPSHRSLDLQPGKASSDKISICQKENIDSVLKPIENVTHCLNAAKVATLPLVHQLEKENI 270

Query: 385 SLDKGCDVHISPEPTFRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTS 444
           +L++ CDV ISPEPTFRSMR         KHVEDEI VDTSLSNWLVESE TPKS S++S
Sbjct: 271 NLEQDCDVLISPEPTFRSMR---------KHVEDEIGVDTSLSNWLVESEITPKSNSNSS 330

Query: 445 SIGNSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYW 504
           SIGNSP+WTRNSAKS+EDRPILGALTLEELRQFS  STPRR+ SRSPEETPIIG+VGSYW
Sbjct: 331 SIGNSPMWTRNSAKSHEDRPILGALTLEELRQFSASSTPRRNISRSPEETPIIGSVGSYW 390

Query: 505 SHTGQDGDSNPGSSCRGLTKTRKSREDERVNWNSTPFLERLDMALASNSAEV 553
           SHTGQD DSNPGSSCRG   TR++RE+ERVNWNSTPFLERLDMALASNSAEV
Sbjct: 391 SHTGQDADSNPGSSCRGPKTTRRNREEERVNWNSTPFLERLDMALASNSAEV 431

BLAST of Spg017181.1 vs. ExPASy TrEMBL
Match: A0A6J1D6S3 (uncharacterized protein LOC111018155 OS=Momordica charantia OX=3673 GN=LOC111018155 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 2.1e-143
Identity = 298/412 (72.33%), Postives = 338/412 (82.04%), Query Frame = 0

Query: 145 VSEASEGVSALEQAQEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDKVVPTK 204
           + + +E V  L++A+E  ++ S+ LN DS++K+ EQIELCH+ EKTSDPI+EDD VVPT+
Sbjct: 27  ILKPNEAVHPLDRAKEEPQLSSVSLNADSLEKVGEQIELCHSGEKTSDPIVEDDAVVPTQ 86

Query: 205 EVENNLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEIDHLD 264
           +VEN L E+KKEEKSG E+DE RGSSNLS AFSL LNHRYA C NS +DDE VEE+D LD
Sbjct: 87  DVENVLDEVKKEEKSGLEDDEPRGSSNLSTAFSLPLNHRYADCRNS-NDDECVEEMDLLD 146

Query: 265 EEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDPSH 324
           E   E RK     DDG+G+S+L ++QESSESLFSLS+ SRKQ+CAVEAGE EVNSP PSH
Sbjct: 147 E--GEGRK-----DDGDGRSELFVQQESSESLFSLSLGSRKQICAVEAGENEVNSPAPSH 206

Query: 325 CSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNATKVKTVPPVHHLEKENISLDK 384
           CS   QP+KAS  +K+ C+N N DSVL PIENVT  LNA +VKTVPPV  LEKENI  +K
Sbjct: 207 CSCGTQPEKASPGQKTGCQNRNADSVLAPIENVTH-LNAARVKTVPPV-RLEKENIDSEK 266

Query: 385 GCDVHISPEPT-FRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSSTSSIG 444
             DV ISPEPT  R +RKLKEN S+ K  EDEIAVDTSLSNWL+E +TTPKSKSS+SSIG
Sbjct: 267 DYDVPISPEPTPKRPLRKLKENHSNSKPAEDEIAVDTSLSNWLIECDTTPKSKSSSSSIG 326

Query: 445 NSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYWSHT 504
           NSP+WTRNSAKSYEDRPILGALTLEELRQFS FSTPRRSRSRSPEETPIIGTVGSYWSHT
Sbjct: 327 NSPMWTRNSAKSYEDRPILGALTLEELRQFSAFSTPRRSRSRSPEETPIIGTVGSYWSHT 386

Query: 505 GQDGDSNPGSS--CRGLTKT-RKSREDERVNWNSTPFLERLDMALASNSAEV 553
            QD D NPGSS  C G TKT RK+REDERVNWNSTPFLERLD ALASNSAEV
Sbjct: 387 RQDADFNPGSSSPCTGSTKTARKNREDERVNWNSTPFLERLDRALASNSAEV 428

BLAST of Spg017181.1 vs. ExPASy TrEMBL
Match: A0A6J1HR12 (uncharacterized protein LOC111465352 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465352 PE=4 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 7.8e-143
Identity = 319/537 (59.40%), Postives = 355/537 (66.11%), Query Frame = 0

Query: 145 VSEASEGVSALEQAQEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDKVVPTK 204
           V +AS+GV ALE+ +EV EVP IDL KDS++KLEEQIELCH TEK S+PIIED+KVVP+K
Sbjct: 30  VFKASDGVCALEKTKEVAEVPPIDLIKDSLEKLEEQIELCHATEKASEPIIEDNKVVPSK 89

Query: 205 EVENNLVEIKKEEKSGREEDESRGSSNLSNAFSLHLNHRYAYCSNSDDDDEYVEEIDHLD 264
           E E+ L EIK E+KSG+EEDES GSSNLS AF+L LNHRY  CSNSDD+DEY EE+DHLD
Sbjct: 90  ENESKLDEIKDEKKSGKEEDESWGSSNLSQAFTLSLNHRYENCSNSDDNDEYAEEMDHLD 149

Query: 265 EEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDPSH 324
           E++EE+RK DTNADDG+G+S LL++QESSESLFSLS  SRKQVC VE  E EV+S D SH
Sbjct: 150 EKEEEKRKDDTNADDGHGESMLLMEQESSESLFSLSFGSRKQVCGVEDAENEVSSLDLSH 209

Query: 325 CSADI------------------------------QPDKASSDKKSVCRNDN-------- 384
              +                               QP+ ASS+KK+ C N+N        
Sbjct: 210 QQENASSEKKTGCWNKNVVGSVLKPIENDTRCLSHQPNNASSEKKTGCWNENVVGSVLNP 269

Query: 385 --------------------------------------------------VDSVLNPIEN 444
                                                             V SVLNPIEN
Sbjct: 270 IENDTRCLSHQPDNASSKKNTGRRNENVVENVTRCLSQQPENASSDKKTDVGSVLNPIEN 329

Query: 445 V-TQCLNATKVKTVPPVHHLEKENISLDKGCDV-HISPEPTFRSMRKLKENRSDLKHVED 504
           V T CL+A KVK +P  HHLEKENISLDKGCDV  ISPEPTFR+MRKLKEN SD KHVED
Sbjct: 330 VTTHCLSAAKVKALPSDHHLEKENISLDKGCDVLLISPEPTFRAMRKLKENCSD-KHVED 389

Query: 505 EIAVDTSLSNWLVESETTPKSKSSTSSIGNSPIWTRNSAKSYEDRPILGALTLEELRQFS 553
           EIAVDTSLSNWLVESETTPKS S++SSIGNSP+WTRNSAKS EDRPILGALTLEE+RQFS
Sbjct: 390 EIAVDTSLSNWLVESETTPKSNSTSSSIGNSPMWTRNSAKSNEDRPILGALTLEEIRQFS 449

BLAST of Spg017181.1 vs. TAIR 10
Match: AT1G04030.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G44040.1); Has 1835 Blast hits to 1511 proteins in 238 species: Archae - 7; Bacteria - 164; Metazoa - 377; Fungi - 135; Plants - 187; Viruses - 22; Other Eukaryotes - 943 (source: NCBI BLink). )

HSP 1 Score: 120.9 bits (302), Expect = 3.1e-27
Identity = 115/383 (30.03%), Postives = 181/383 (47.26%), Query Frame = 0

Query: 189 KTSDPIIEDDKVVPTKEVENNLVEIKKEEKSGREEDE-SRGSSNLSNAFSLHLNHRYAYC 248
           KT + ++ ++ V  ++E    +   K+  KS + +D+    +SN S ++    NHRY  C
Sbjct: 86  KTYEHVVSEESVELSEEKNEEVESEKRSLKSSKTDDQIIEVASNSSGSYP--ENHRYKNC 145

Query: 249 SNSDDD---DEYVEEIDHLDEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSR 308
             SDDD   DE+      LDE++E       + D  +  +K +  Q+  +    +    R
Sbjct: 146 RESDDDIEEDEFDCSDSDLDEDEEYYSDVGFSEDSLHNPTKEVYTQDIGDKTEEIDSKLR 205

Query: 309 KQVCAVEAGEIEVNSPDPSHCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQCLNA- 368
           +    V  G                              + +   VLNP+EN+TQ  +A 
Sbjct: 206 RSNETVRDG-----------------------------NHYDGQGVLNPVENLTQWKSAK 265

Query: 369 TKVKTVPPVHHLEKENISLDKG--------------CDVHISPEPTFRSMRKLKENRSDL 428
           +K +T       E  N   D+                D+ +S +P  R   K   N+   
Sbjct: 266 SKGRTKQKQSQKENSNFIADQEEKRDSSSFGTDPQIDDITLSVKPKCRIEPKKLRNQ--- 325

Query: 429 KHVEDEIAVDTSLSNWLVESETTPKSKSST------SSIGNSPIWTRNSAKSYEDRPILG 488
                E+AVD SLS WL  SE+  +  S++        + ++  +++    +++DRP+L 
Sbjct: 326 -----ELAVDASLSTWLSTSESGSECNSASMYTLTPEKLKSTSCYSKPLRINHDDRPVLC 385

Query: 489 ALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGSYWSHTGQDGDSNPGSSCRGLTKT-R 546
           ALTLE+++QFS  STPR+S S+SP+ETPIIGTVG YW +  +  D    SS +G+  T  
Sbjct: 386 ALTLEDIKQFSATSTPRKSPSKSPDETPIIGTVGGYWGNRSKAIDCGSASSFKGIPNTSS 429

BLAST of Spg017181.1 vs. TAIR 10
Match: AT5G44040.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G04030.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 97.8 bits (242), Expect = 2.8e-20
Identity = 116/363 (31.96%), Postives = 169/363 (46.56%), Query Frame = 0

Query: 145 VSEASEGVSALEQAQEVLEVPSIDLNKDSVKKLEEQIELCHTTEKTSDPIIEDDKVVPTK 204
           VS     V+ +E+   V   P  D+  D V++ +      +    T D  ++  + +   
Sbjct: 68  VSPKDNHVNVVEKVPTVSVTPITDI-CDKVEEKQSPSPSPNRKRVTFDTNVKTYEHIAVD 127

Query: 205 EVENNLVEIKKEEKSGREEDESRGSSNLSNAF-SLHLNHRYAYCSNSDDDDEYVEEIDHL 264
           E      E K+E KS +    S GS   SN+  S   NHRY  C  SDD++E V + D  
Sbjct: 128 ESVELFEEKKEEVKSRQARCSSEGSDVTSNSSGSYPSNHRYQNCRESDDEEEDVTDCDDS 187

Query: 265 DEEQEEERKADTNADDGNGKSKLLIKQESSESLFSLSISSRKQVCAVEAGEIEVNSPDPS 324
           D E       DT+ DD      LL     ++  +   + +  +V   E  EI  N  D  
Sbjct: 188 DLE-------DTDDDD----CGLLDDDYYNDDNYEDKLHNWDKVVYTE--EIADNVMDIE 247

Query: 325 HCSADIQPDKASSDKKSVCRNDNVDSVLNPIENVTQ-----CLNATKVKTVPPVHHLEKE 384
                   +K S   +   R+  V++VLNPIEN++Q         T  +T P   ++   
Sbjct: 248 RVE-----EKGSVSVRD--RSGYVNAVLNPIENLSQWKAVKAKGRTTTQTQPRKENVIIA 307

Query: 385 NISLDKGCDVHISPEPTFRSMRKLKENRSDLKHVEDEIAVDTSLSNWLVESETTPKSKSS 444
           + SL+   D       TF   RK ++     K    EIAVD SLS WL  S+TT    SS
Sbjct: 308 SFSLESQVD---DLSSTFSLNRKSRDETE--KQRTQEIAVDASLSTWLSTSQTTTSGCSS 367

Query: 445 TSSIGNSPIWTRNSAKSYEDRPILGALTLEELRQFSTFSTPRRSRSRSPEETPIIGTVGS 502
             +  +         + +++RPILGALT EE++QFS  ++PR+S SRSP E+PIIGTVG 
Sbjct: 368 VETTMSEKKKYSKLVQCHDERPILGALTAEEIKQFSATNSPRKSPSRSP-ESPIIGTVGG 403