Spg008747.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg008747.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Calcium-binding EF-hand family protein
Location	scaffold10: 35313151 .. 35313651 (+)
Sequence length	501
RNA-Seq Expression	Spg008747.1
Synteny	Spg008747.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCAATTAGCTCCAACGACTTGCACAGGATCTTCAACAAGCTCGACAAGAATGGCGATGGCCTCATTTGCCTACGCGAGCTCAACTGGCTCCTCGACAGGATCGGCATCGAACTGACCATGGAGGAGTTGGAGTCTTCTCTAGAGAAGCCGACCCTCAACTTCAACGAGTTCTTGTTCTTTTATCATTCGATATCAAAACAAAACAAAGGCGAGTGCAAGGGAGGTGTAGGCGAGGACGAGAATGAGACGATGCACGAGCATGGAGGAGAGGAGGATATTGACATTGTATATATGGCGTTCAAAGTGTTTGACATCAACGATGATGGCTTCATTTCTTGTGAGGAGCTTCAAAATGTGTTGTCAAGGCTAGAGCTTTGGGACGCAAACGGAAGCGACATCGATTGCAAAAGCATGATTCGAGCTTACGACACCAATTTGGATGGAAAGCTCGATTTCGAGGAGTTTAAGAACATGATGTTGCTTACAAGTTCTTGA

mRNA sequence

ATGTCTCCAATTAGCTCCAACGACTTGCACAGGATCTTCAACAAGCTCGACAAGAATGGCGATGGCCTCATTTGCCTACGCGAGCTCAACTGGCTCCTCGACAGGATCGGCATCGAACTGACCATGGAGGAGTTGGAGTCTTCTCTAGAGAAGCCGACCCTCAACTTCAACGAGTTCTTGTTCTTTTATCATTCGATATCAAAACAAAACAAAGGCGAGTGCAAGGGAGGTGTAGGCGAGGACGAGAATGAGACGATGCACGAGCATGGAGGAGAGGAGGATATTGACATTGTATATATGGCGTTCAAAGTGTTTGACATCAACGATGATGGCTTCATTTCTTGTGAGGAGCTTCAAAATGTGTTGTCAAGGCTAGAGCTTTGGGACGCAAACGGAAGCGACATCGATTGCAAAAGCATGATTCGAGCTTACGACACCAATTTGGATGGAAAGCTCGATTTCGAGGAGTTTAAGAACATGATGTTGCTTACAAGTTCTTGA

Coding sequence (CDS)

ATGTCTCCAATTAGCTCCAACGACTTGCACAGGATCTTCAACAAGCTCGACAAGAATGGCGATGGCCTCATTTGCCTACGCGAGCTCAACTGGCTCCTCGACAGGATCGGCATCGAACTGACCATGGAGGAGTTGGAGTCTTCTCTAGAGAAGCCGACCCTCAACTTCAACGAGTTCTTGTTCTTTTATCATTCGATATCAAAACAAAACAAAGGCGAGTGCAAGGGAGGTGTAGGCGAGGACGAGAATGAGACGATGCACGAGCATGGAGGAGAGGAGGATATTGACATTGTATATATGGCGTTCAAAGTGTTTGACATCAACGATGATGGCTTCATTTCTTGTGAGGAGCTTCAAAATGTGTTGTCAAGGCTAGAGCTTTGGGACGCAAACGGAAGCGACATCGATTGCAAAAGCATGATTCGAGCTTACGACACCAATTTGGATGGAAAGCTCGATTTCGAGGAGTTTAAGAACATGATGTTGCTTACAAGTTCTTGA

Protein sequence

MSPISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFLFFYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNVLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS

Homology

[Close]

BLAST of Spg008747.1 vs. NCBI nr
Match: XP_022141411.1 (probable calcium-binding protein CML44 [Momordica charantia])

HSP 1 Score: 283.5 bits (724), Expect = 1.2e-72
Identity = 135/166 (81.33%), Postives = 154/166 (92.77%), Query Frame = 0


Query: 1   MSPISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFL 60
           MSPI SNDL +IFNKLDKN DGL+CL+EL+WLLDRIGI+LTMEELESSLEKP+L+FNEFL
Sbjct: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60

Query: 61  FFYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQN 120
           FFY SISK+NKGEC GGVG+DENE +  H GEED+DIVYMAFKVFD+NDDGFISCEEL++
Sbjct: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120

Query: 121 VLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 167
           VL RLELWD+N SD +C++MIRAYDTNLDGKLDFEEFKNMMLLT+S
Sbjct: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

[Close]

BLAST of Spg008747.1 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 263.8 bits (673), Expect = 9.7e-67
Identity = 133/168 (79.17%), Postives = 149/168 (88.69%), Query Frame = 0


Query: 1   MSPISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFL 60
           MS IS NDLHRIFNKLDKNGDGLICL+EL WL DRIGI+LTMEELES LEKP+L+FNEFL
Sbjct: 11  MSSISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFL 70

Query: 61  FFYHSISKQNKGECKGGVG--EDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEEL 120
           FFY SISKQNKGECKGGVG  +D++        EE+++IVYMAFKVFD+N DGFISC+EL
Sbjct: 71  FFYESISKQNKGECKGGVGCLQDDH--------EEEMEIVYMAFKVFDMNGDGFISCDEL 130

Query: 121 QNVLSRLELWDANGSDID-CKSMIRAYDTNLDGKLDFEEFKNMMLLTS 166
           +NVLSRLELWDAN +DID C+SMIRAYDTNLDGKLDFEEFKNMMLLT+
Sbjct: 131 ENVLSRLELWDANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 170

[Close]

BLAST of Spg008747.1 vs. NCBI nr
Match: KAG6602129.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 257.7 bits (657), Expect = 6.9e-65
Identity = 132/167 (79.04%), Postives = 141/167 (84.43%), Query Frame = 0


Query: 1   MSP-ISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEF 60
           MSP IS NDL RIFNKLDKN DGLICL ELNWLLD IG+ELTME+L S LEKP+L+FNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQ 120
           LFFY SISKQNKGECKGGV +        +  EED+DIVYMAFKVFD+NDDGFISCEELQ
Sbjct: 61  LFFYESISKQNKGECKGGVSD-------VNAREEDVDIVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 NVLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 167
            VLSRLELWD N +D DCKSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 LVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Spg008747.1 vs. NCBI nr
Match: KAG6578937.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 257.7 bits (657), Expect = 6.9e-65
Identity = 126/164 (76.83%), Postives = 145/164 (88.41%), Query Frame = 0


Query: 3   PISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFLFF 62
           PISSNDL RIFN+LDKN DGLIC++EL WLLD IGI+LTMEELES LEKP+L+F+EFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YHSISKQNKGECKGGVGEDENETMHEHGG-EEDIDIVYMAFKVFDINDDGFISCEELQNV 122
           Y SISKQNKGECKGG+       ++EHG  EED++IVYMAFKVFD+N DGFISCEEL+NV
Sbjct: 64  YESISKQNKGECKGGL-------VYEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENV 123

Query: 123 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 166
           LSRLELWDAN +++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 124 LSRLELWDANKNEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Spg008747.1 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 257.7 bits (657), Expect = 6.9e-65
Identity = 127/164 (77.44%), Postives = 144/164 (87.80%), Query Frame = 0


Query: 3   PISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFLFF 62
           PISSNDL RIFN+LDKN DGLIC++EL WLLD IGI+LTMEELES LEKP+L+F+EFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YHSISKQNKGECKGGVGEDENETMHEHGG-EEDIDIVYMAFKVFDINDDGFISCEELQNV 122
           Y SISKQNKGECKGG+       + EHG  EED++IVYMAFKVFD+N DGFISCEEL+NV
Sbjct: 64  YESISKQNKGECKGGL-------VDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENV 123

Query: 123 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 166
           LSRLELWDAN S++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 124 LSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Spg008747.1 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 9.2e-28
Identity = 74/161 (45.96%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 4   ISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIG-IELTMEELESSLEKPTLNFNEFLFF 63
           I++NDL R+F  LDKN DGL+ L EL W+LD++G  E T +ELE  + K +L+ +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YHSISKQNKGECKG-GVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNV 123
           Y+     +KG  K   V  D +E             +  AF VFD+N DG+IS EEL++V
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDEA------------IARAFNVFDVNGDGYISAEELRDV 125

Query: 124 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMML 163
           L RL  ++      DC  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 LERLG-FEEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Spg008747.1 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.2e-20
Identity = 65/160 (40.62%), Postives = 92/160 (57.50%), Query Frame = 0


Query: 9   LHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLE------KPTLNFNEFLFF 68
           L RIF+  DKNGDG I + EL+  L R+G+   + +L+S++E         LNF++F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNVL 128
           + ++     G   GG GE+E++       E D+     AFKVFD N DGFIS  ELQ VL
Sbjct: 90  HKTLDDSFFGGACGG-GENEDDPSSAAENESDL---AEAFKVFDENGDGFISARELQTVL 149

Query: 129 SRLELWDANGSDID-CKSMIRAYDTNLDGKLDFEEFKNMM 162
            +L L    G +++  + MI + D N DG++DF EFKNMM
Sbjct: 150 KKLGL--PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Spg008747.1 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.3e-18
Identity = 64/162 (39.51%), Postives = 96/162 (59.26%), Query Frame = 0


Query: 9   LHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPT------LNFNEFLFF 68
           L RIF+  DKN DG+I + EL+  L+ +G+E  + ELES+++  T      L F +F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YHSISKQNKGECKGGVGEDENETMHEHG--GEEDIDIVYMAFKVFDINDDGFISCEELQN 128
           + S++  +     GG  ED+NE         +E+ D  +  FKVFD + DG+IS  ELQ 
Sbjct: 101 HQSLN--DSYFAYGGEDEDDNEEDMRKSILSQEEAD-SFGGFKVFDEDGDGYISARELQM 160

Query: 129 VLSRLELWDANGSDID-CKSMIRAYDTNLDGKLDFEEFKNMM 162
           VL +L    + GS+ID  + MI + D+N DG++DF EFK+MM
Sbjct: 161 VLGKLGF--SEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

[Close]

BLAST of Spg008747.1 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 59/162 (36.42%), Postives = 87/162 (53.70%), Query Frame = 0


Query: 9   LHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSL------EKPTLNFNEFLFF 68
           LHR+F+  DKN DG I + EL+  L R+G++    +L+S++      +K  L F++F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YHSISKQ---NKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQ 128
           + ++ +     +G C  G  E + E                AF VFD + DGFIS  ELQ
Sbjct: 89  HKTLDESFFGGEGSCCDGSPESDLEE---------------AFNVFDEDGDGFISAVELQ 148

Query: 129 NVLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 162
            VL +L L +A G     + MI + D+N DG++DF EFKNMM
Sbjct: 149 KVLKKLGLPEA-GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of Spg008747.1 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-17
Identity = 62/175 (35.43%), Postives = 88/175 (50.29%), Query Frame = 0


Query: 4   ISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLN------FN 63
           + S +L ++F   DKNGDG I  +EL       GI +  +EL+++++K   N        
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYHSISKQNKG-----------ECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFD 123
           EF   Y SI   +               +GG  +DE+E M E            AF VFD
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMRE------------AFNVFD 120

Query: 124 INDDGFISCEELQNVLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 162
            N DGFI+ +EL++VLS L L     +D DC+ MI   D + DG++DF+EFK MM
Sbjct: 121 QNGDGFITVDELRSVLSSLGLKHGRTAD-DCRRMISMVDADGDGRVDFKEFKQMM 162

[Close]

BLAST of Spg008747.1 vs. ExPASy TrEMBL
Match: A0A6J1CJ36 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111011820 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 5.7e-73
Identity = 135/166 (81.33%), Postives = 154/166 (92.77%), Query Frame = 0


Query: 1   MSPISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFL 60
           MSPI SNDL +IFNKLDKN DGL+CL+EL+WLLDRIGI+LTMEELESSLEKP+L+FNEFL
Sbjct: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60

Query: 61  FFYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQN 120
           FFY SISK+NKGEC GGVG+DENE +  H GEED+DIVYMAFKVFD+NDDGFISCEEL++
Sbjct: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120

Query: 121 VLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 167
           VL RLELWD+N SD +C++MIRAYDTNLDGKLDFEEFKNMMLLT+S
Sbjct: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

[Close]

BLAST of Spg008747.1 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.4e-65
Identity = 127/164 (77.44%), Postives = 144/164 (87.80%), Query Frame = 0


Query: 3   PISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFLFF 62
           PISSNDL RIFN+LDKN DGLIC++EL WLLD IGI+LTMEELES LEKP+L+F+EFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YHSISKQNKGECKGGVGEDENETMHEHGG-EEDIDIVYMAFKVFDINDDGFISCEELQNV 122
           Y SISKQNKGECKGG+       + EHG  EED++IVYMAFKVFD+N DGFISCEEL+NV
Sbjct: 64  YESISKQNKGECKGGL-------VDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENV 123

Query: 123 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 166
           LSRLELWDAN S++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 124 LSRLELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Spg008747.1 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.7e-64
Identity = 126/164 (76.83%), Postives = 144/164 (87.80%), Query Frame = 0


Query: 3   PISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEFLFF 62
           PISSNDL RIFN+LDKNGDGLIC++EL WLLD IGI+LTMEELES LEKP+L+F+EFL F
Sbjct: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63

Query: 63  YHSISKQNKGECKGG-VGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNV 122
           Y S+SKQNKGECKGG V E E+E       +ED++IVYMAFKVFD+N DGFISCEEL+NV
Sbjct: 64  YESMSKQNKGECKGGLVDEHEDE-------DEDVEIVYMAFKVFDVNGDGFISCEELENV 123

Query: 123 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 166
           LSRLELWDAN S++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 124 LSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Spg008747.1 vs. ExPASy TrEMBL
Match: A0A6J1H868 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111460440 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.2e-64
Identity = 130/167 (77.84%), Postives = 140/167 (83.83%), Query Frame = 0


Query: 1   MSP-ISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEF 60
           MSP IS NDL RIFNKLDKN DGLICL ELNWLLD IG+ELTME+L S LEKP+L+FNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQ 120
           LFFY SISKQNKGECKGGV +        +  EED+D+VYMAFKVFD+NDDGFISCEELQ
Sbjct: 61  LFFYESISKQNKGECKGGVSD-------VNAREEDVDVVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 NVLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 167
            VLSRLELWD N +D DCKSMIRAYDTNLDG LDFEEFKNMM L SS
Sbjct: 121 LVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

[Close]

BLAST of Spg008747.1 vs. ExPASy TrEMBL
Match: A0A6J1JGU4 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111486883 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.8e-64
Identity = 129/167 (77.25%), Postives = 142/167 (85.03%), Query Frame = 0


Query: 1   MSP-ISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLNFNEF 60
           MSP I+ NDL RIFNKLDKN DGLICL EL+WLLD IG+ELTME+L S LEKP+L+FNEF
Sbjct: 1   MSPIININDLLRIFNKLDKNRDGLICLHELSWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQ 120
           LFFY SISKQNKGECKGGV +        +  E+D+DIVYMAFKVFD+NDDGFISCEELQ
Sbjct: 61  LFFYESISKQNKGECKGGVSD-------VNAREDDVDIVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 NVLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 167
           +VLSRLE+WD N S IDCKSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 HVLSRLEIWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Spg008747.1 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 124.8 bits (312), Expect = 6.5e-29
Identity = 74/161 (45.96%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 4   ISSNDLHRIFNKLDKNGDGLICLRELNWLLDRIG-IELTMEELESSLEKPTLNFNEFLFF 63
           I++NDL R+F  LDKN DGL+ L EL W+LD++G  E T +ELE  + K +L+ +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YHSISKQNKGECKG-GVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNV 123
           Y+     +KG  K   V  D +E             +  AF VFD+N DG+IS EEL++V
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDEA------------IARAFNVFDVNGDGYISAEELRDV 125

Query: 124 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMML 163
           L RL  ++      DC  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 LERLG-FEEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Spg008747.1 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 99.8 bits (247), Expect = 2.3e-21
Identity = 65/160 (40.62%), Postives = 92/160 (57.50%), Query Frame = 0


Query: 9   LHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLE------KPTLNFNEFLFF 68
           L RIF+  DKNGDG I + EL+  L R+G+   + +L+S++E         LNF++F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNVL 128
           + ++     G   GG GE+E++       E D+     AFKVFD N DGFIS  ELQ VL
Sbjct: 90  HKTLDDSFFGGACGG-GENEDDPSSAAENESDL---AEAFKVFDENGDGFISARELQTVL 149

Query: 129 SRLELWDANGSDID-CKSMIRAYDTNLDGKLDFEEFKNMM 162
            +L L    G +++  + MI + D N DG++DF EFKNMM
Sbjct: 150 KKLGL--PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Spg008747.1 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 91.3 bits (225), Expect = 8.0e-19
Identity = 59/162 (36.42%), Postives = 87/162 (53.70%), Query Frame = 0


Query: 9   LHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSL------EKPTLNFNEFLFF 68
           LHR+F+  DKN DG I + EL+  L R+G++    +L+S++      +K  L F++F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YHSISKQ---NKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQ 128
           + ++ +     +G C  G  E + E                AF VFD + DGFIS  ELQ
Sbjct: 89  HKTLDESFFGGEGSCCDGSPESDLEE---------------AFNVFDEDGDGFISAVELQ 148

Query: 129 NVLSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 162
            VL +L L +A G     + MI + D+N DG++DF EFKNMM
Sbjct: 149 KVLKKLGLPEA-GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of Spg008747.1 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 86.7 bits (213), Expect = 2.0e-17
Identity = 61/160 (38.12%), Postives = 82/160 (51.25%), Query Frame = 0


Query: 8   DLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLN------FNEFLF 67
           +L RIF   D+NGDG I  +ELN  L+ +GI +  ++L   +EK  LN        EF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 68  FYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNV 127
            Y +I ++           DE E M E            AF VFD N DGFI+ EEL++V
Sbjct: 65  LYQTIMEE----------RDEEEDMRE------------AFNVFDQNRDGFITVEELRSV 124

Query: 128 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 162
           L+ L L      + DCK MI   D + DG ++F+EFK MM
Sbjct: 125 LASLGLKQGRTLE-DCKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Spg008747.1 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 84.3 bits (207), Expect = 9.8e-17
Identity = 58/160 (36.25%), Postives = 83/160 (51.88%), Query Frame = 0


Query: 8   DLHRIFNKLDKNGDGLICLRELNWLLDRIGIELTMEELESSLEKPTLN------FNEFLF 67
           +L R+F   DKNGDG I  +EL+  L  +GI +  +EL   +EK  +N       +EF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYHSISKQNKGECKGGVGEDENETMHEHGGEEDIDIVYMAFKVFDINDDGFISCEELQNV 127
            Y +I  +          ++E E M E            AF VFD N DGFI+ +EL+ V
Sbjct: 65  LYKTIMDEE---------DEEEEDMKE------------AFNVFDQNGDGFITVDELKAV 124

Query: 128 LSRLELWDANGSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 162
           LS L L      D DCK MI+  D + DG+++++EF+ MM
Sbjct: 125 LSSLGLKQGKTLD-DCKKMIKKVDVDGDGRVNYKEFRQMM 142

The following BLAST results are available for this feature:

BLAST of Spg008747.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141411.1	1.2e-72	81.33	probable calcium-binding protein CML44 [Momordica charantia]	[more]
XP_038885742.1	9.7e-67	79.17	probable calcium-binding protein CML44 [Benincasa hispida]	[more]
KAG6602129.1	6.9e-65	79.04	putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s...	[more]
KAG6578937.1	6.9e-65	76.83	putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023551499.1	6.9e-65	77.44	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg008747.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	9.2e-28	45.96	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9SVG9	3.2e-20	40.63	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
P43187	1.3e-18	39.51	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
Q9FI19	1.1e-17	36.42	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q2QVI1	1.5e-17	35.43	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Spg008747.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJ36	5.7e-73	81.33	probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1FLU5	3.4e-65	77.44	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1K1Y7	1.7e-64	76.83	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893...	[more]
A0A6J1H868	2.2e-64	77.84	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1JGU4	2.8e-64	77.25	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114868...	[more]

BLAST of Spg008747.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	6.5e-29	45.96	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	2.3e-21	40.63	calmodulin like 42	[more]
AT5G44460.1	8.0e-19	36.42	calmodulin like 43	[more]
AT3G07490.1	2.0e-17	38.13	ARF-GAP domain 11	[more]
AT1G05990.1	9.8e-17	36.25	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 8..36 e-value: 0.027 score: 23.6 coord: 136..164 e-value: 0.0015 score: 27.8 coord: 97..125 e-value: 0.026 score: 23.7
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 12..29 e-value: 2.2E-4 score: 20.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 99..161 e-value: 9.6E-10 score: 38.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 93..128 score: 12.644413
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 11.807551
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 132..166 score: 11.165957
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 101..161 e-value: 6.54861E-12 score: 55.6317
None	No IPR available	GENE3D	1.10.238.10		coord: 68..161 e-value: 7.8E-18 score: 66.5
None	No IPR available	GENE3D	1.10.238.10		coord: 5..67 e-value: 9.2E-9 score: 37.3
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 1..166
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..166
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 106..118
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 8..162

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg008747	Spg008747	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg008747.1-cds	Spg008747.1-cds-scaffold10:35313151..35313651	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg008747.1	Spg008747.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding