Spg004546.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg004546.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	DNA primase
Location	scaffold9: 9455803 .. 9459706 (-)
Sequence length	432
RNA-Seq Expression	Spg004546.1
Synteny	Spg004546.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAGATTCCGTCTCTTTATCAACCAAAGATGGGTCTCCACTTACGTTAGTCGAGACTTTCTTGGTCGTCATGGTGAAAGCGTCCCAAAATTCGAGCGCTCTGATACCAATTTGATGGAGCCGAAAGTATGAATAACCTCAAAATATATTCAATGATCAAAACTAAAGGCTATGAGCCTTTAAATAGGCTTATACAAGATGCGGTTAGCCTAACAAACTAACTAACCAAGAAGCACATGGCAAACACATGGCTTAAAAAGGATAACAACAAACAAATAAAATACTAAGCCAGTGTTCGGCTACATCACTCAGTCTCTCGAATACTGAGTTTATGTTTACTGCTTTGTTAGTCTCTTGTTATGAACTTATCTTGCTGTTTGGCTAGTTGAACTGCCCCCCTGAACCAGTGAAGAACTCTCATTGACAGGGCTGGAATTCCATCTTGAGTGTGTTATTGTGTTTGTGTGTTGGTATTTTCTGCTTTTACAGGTTCAAGGTGAGGAAAATAGAAGGAGATTCATAACAGTCTACTAAAGCCAAAACAAAGTTGTAACCCCATTATATCCTAGGGAGGCCAAGAACATAATCCGTAGACAAATCCTCCCAAATTGAATTAGGGATGGGGAGTGTAAAGGTCCGTGTTTTGACTTGAACCCTTAGAAGTTTGACAAATAAAACAATGTTTGACAAAATTTGCTATATCCGTTTTTAATTGAGGCCAAAAGAATTTTGAAGAAAGGGACTCAAAGATTTTATCTCGGCCAAAATGGTTAGCTAACTCACCCGAGTATAGTTCTTTTAAAAGTGCTTCCTATGAGAAGTTTGTGGTATACAGAGTGTTTTAAAAAAACCGTCAACAATGTGGACATCATAACAAGGTAAGTGATTCAAGCATTTAGTCCAAATGTCTTTCAAAGTCTGTCAGTTTCATACAATTGTGGGAGATGATTATTTAGTCCAAATGTCTTCAGCTGACGAACAGCCATAACAAACTAAAATAATAGCCCAAGAAGCAGCCAAAAAGATTTGCAAAAACAGATAAAACAATGATAGACGCAAATGGTAAAAATAGCTAAAATACCCGAATGTCCTTGCATCACTTTACCCAGGTAGATCTACTTTATATTCTAAGGACTTCACTCAACAGTCAAAAGCCCCCTTCCCCCTTTCTAAATCAGCTCACCTTTGTACCACTACCTTTTGTCCTCCCATGGTAACTTTTTACACCTTTTGTCCTATGGTAACTTCTTAAAAGCTTAACTGATACCCACTACCCTTTCCTTCAAAATCCAGCCTAATTATCAAAGCTGCTTGCTTCCCTTGGTCCCACACTTATGGACGATTACTCTCATCTTTCCTCTAGCAAGGGCCCCTTATCAACAACTGTGTCCAATCAACAATCCCATCTACCCCCTTCTGTACCAAAGATTAAAAAAAGTTTATTTCTTCCATCTCCAAAATTTCCATCAAAATTTTCTTTGTAAATGGCACCAAACACTCCACCTCCATGCACACCTGTCCTTGTACTAGACTTCGATGACTATCTATGAAGCCCTTTCCTAGAGATGAGAGAACATTAAATAACATAAAGGAAGTCTTTTGATATGATAGGAAGAATAGAATTGGAGTAGAAACTGTTTTGAAACTTAAAGGACAAGAAATACTAAAATATCTCAGGTAATTCGAAGAACTTAGACATCTCCCTTTGGGGCAAGCACAACCCAAACATCCAAAAAATTGATTGCCTTCACTCCCTCTCACTATTCATAATCACCTCTTACTACAGAACTTCTTACTTCATGACTAATATGCCTTAGTATCCTAATCATATTTCTAACATTATTGCTATCTTACGATGAAGGAGGTCATGTTTTACCCTCCTTTTAGAGAGAAGGGTAGTGTCATTTGGAGGGCTGGCTTTTGGGTGTTTTCATGGGACATTTGAATTGAGGGAAATAATAGTCTTTAGGATGTGGAGAGATCTTAGGAGGAGGTTTCGTCGCGAGCCTAGTTTAATGTCTTTTTGGGTGTATATCTACGAAGACTTTTATAATTATTCGTTAGGTCTTAGTTTTTTGGATTAGAGCACTCTTTGTCTAGTTAGATTAGTTCCTATTTGGCTCTTTTTGGACTTTTGTTTCTATAGGCCCATTTTTTGCTAGTCCATGTTGCTTGGTTTTGATGGGCTCTTTTGTATTCTTTCATTTTCTTCTCAACAAATGTTTGGTTTATGAAGATATAAATACACACACATGCATACATTTATCATATAGAATCCAAGAAGTTGTAATCTTTATTTTTAATTGTCTTTCTCAATCATCTAGCTTCCATTTCCCCTTCTTTATTTCTTTCCAGTCATGTTTTGTGTGGCTGCCATTAGATATGTCTTGGTGCAACATTTTCTTTTTTTCTTTCCAGTCATCTAGCTTATTGAATGGGTAGTGTAGAATCCAAATTCTAACAACCGGCAACTCTGCTACATTTAGATTATAATTATGACGTTATCCATTGGATCAGGCACAAGGAATGCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTACCCTAGGCTTGATTCGGAGGTATGTGTATTACCTGCCTTTATCTTACCTAGATTTTTCACCCATCTTTTGGAATTAGGTCATTCTATTCTAGCAATTGCATTTGTTACATACTGGATCTCTAATTGGCATGCATGGGATTGTGACTAAAAATTCAAATTTACTTGTTAAATCTGATTTTAATTTACTCCCTTGATGAGGCAAGTTCTTTTTTCTTCAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTTATTGTTGGAAATATTTGCTAGTACTAGATGATTAGCAGTGACTTGCAGAAACCTCATTATGGTTTCAATGTAAAATTGAATTAGTTAAGGAAGGTTCACTTGATTTGAAACCCTGAAAGTACTTTGGTTGGCACGAAGGATGCAATGATTTCACTTTTGTGAAGAGAACATGTGTTTACATATTATATTATTATTGATTCTTATACCTTCTTATTCCAGGTCGTGTTTGTGTCCCTATCAACCCTAAACGATGTGATGAGTTTGATCCAACTACTGTACCAACTGTTGCCCAGGTAAAACAAGAAATAAAGGCGTAAACATAAATGTAATGTTTTTGCACACTGATTTGCAGCAAGGAAAATGTTTAATTTTCAATTTAATACTTGGTAATTTGTTATGGTTAGAAATAACTAAGACAAAGTTGAACATTGAAATGTGAAATTGTTATTGTATCCAACTTTTAGAAGTCTGTGAAAAACCTGCTCATGCTAATTGGATTAAGGCGATTGCATCTTCTAGCATAAATTCTTTAAACAAGTTGCTAGCAGCTCTTTCTTTGTGCATTCTGGCTGAAGAAAGATGATCAAAATTTGTATGGTTAAACCGTGGTATATAGCAAATGTTCCATTGTGGTTTATGGTAATTTGGCTTCTGTAGAATAACTAGAAATTAATGATGGATTGCAGCTTCTAGAAGAACTTAATGCAGGGGATATGATAGTAGATGGTGAGACTGGTATGTTATTTGCCATCTTCATTATAGATTCAGGTTTCTGCTTTATTTGCTTGTAGCTTCGGCGTATAATTTAATATTAGTGATTAACAAATGCTGTTATAATCTTTTTACTTCGTGACAGATTGGGATAGAACCTCACTTGGGGAATCGATTAGGTTTTTTAGATCGTCATTCTTACAGCCATTACTGAAATCATGCAAGGTAATTTTTTTCTAATATATGTAGTAAATCTCTGACCTTTCAAGTTTTTGATCATCACTCTTGTAGGCTCGAAATTGTGCAGGAGTTACTATCACAGTCTGTTAGGGCTAGGCTACTAG

mRNA sequence

ATGGGTAGATTCCGTCTCTTTATCAACCAAAGATGGGTCTCCACTTACGTTAGTCGAGACTTTCTTGGTCGTCATGGTGAAAGCGTCCCAAAATTCGAGCGCTCTGATACCAATTTGATGGAGCCGAAAGCACAAGGAATGCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTACCCTAGGCTTGATTCGGAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCGTGTTTGTGTCCCTATCAACCCTAAACGATGTGATGAGTTTGATCCAACTACTGTACCAACTGTTGCCCAGCTTCTAGAAGAACTTAATGCAGGGGATATGATAGTAGATGGTGAGACTGGCTCGAAATTGTGCAGGAGTTACTATCACAGTCTGTTAGGGCTAGGCTACTAG

Coding sequence (CDS)

ATGGGTAGATTCCGTCTCTTTATCAACCAAAGATGGGTCTCCACTTACGTTAGTCGAGACTTTCTTGGTCGTCATGGTGAAAGCGTCCCAAAATTCGAGCGCTCTGATACCAATTTGATGGAGCCGAAAGCACAAGGAATGCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTACCCTAGGCTTGATTCGGAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCGTGTTTGTGTCCCTATCAACCCTAAACGATGTGATGAGTTTGATCCAACTACTGTACCAACTGTTGCCCAGCTTCTAGAAGAACTTAATGCAGGGGATATGATAGTAGATGGTGAGACTGGCTCGAAATTGTGCAGGAGTTACTATCACAGTCTGTTAGGGCTAGGCTACTAG

Protein sequence

MGRFRLFINQRWVSTYVSRDFLGRHGESVPKFERSDTNLMEPKAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDPTTVPTVAQLLEELNAGDMIVDGETGSKLCRSYYHSLLGLGY

Homology

[Close]

BLAST of Spg004546.1 vs. NCBI nr
Match: KAA0039338.1 (DNA primase, small subunit [Cucumis melo var. makuwa] >TYK00521.1 DNA primase, small subunit [Cucumis melo var. makuwa])

HSP 1 Score: 170.6 bits (431), Expect = 9.6e-39
Identity = 79/90 (87.78%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           KAQGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+ CDEFDP
Sbjct: 335 KAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPEHCDEFDP 394

Query: 103 TTVPTVAQLLEELNAGDMIVDGETGSKLCR 133
           TTVPTVAQLLEELNA DM VD ETG K+ R
Sbjct: 395 TTVPTVAQLLEELNAADMEVDSETGLKIKR 424

[Close]

BLAST of Spg004546.1 vs. NCBI nr
Match: XP_022973496.1 (DNA primase small subunit [Cucurbita maxima])

HSP 1 Score: 166.0 bits (419), Expect = 2.4e-37
Identity = 76/84 (90.48%), Postives = 80/84 (95.24%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           K+QGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+RCDEFDP
Sbjct: 315 KSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPERCDEFDP 374

Query: 103 TTVPTVAQLLEELNAGDMIVDGET 127
           TTVPTVAQLLEELNA DM VD ET
Sbjct: 375 TTVPTVAQLLEELNAADMEVDSET 398

[Close]

BLAST of Spg004546.1 vs. NCBI nr
Match: XP_023535939.1 (DNA primase small subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 166.0 bits (419), Expect = 2.4e-37
Identity = 76/84 (90.48%), Postives = 80/84 (95.24%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           K+QGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+RCDEFDP
Sbjct: 320 KSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPERCDEFDP 379

Query: 103 TTVPTVAQLLEELNAGDMIVDGET 127
           TTVPTVAQLLEELNA DM VD ET
Sbjct: 380 TTVPTVAQLLEELNAADMEVDSET 403

[Close]

BLAST of Spg004546.1 vs. NCBI nr
Match: KAG6592333.1 (DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 166.0 bits (419), Expect = 2.4e-37
Identity = 76/84 (90.48%), Postives = 80/84 (95.24%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           K+QGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+RCDEFDP
Sbjct: 320 KSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPERCDEFDP 379

Query: 103 TTVPTVAQLLEELNAGDMIVDGET 127
           TTVPTVAQLLEELNA DM VD ET
Sbjct: 380 TTVPTVAQLLEELNAADMEVDSET 403

[Close]

BLAST of Spg004546.1 vs. NCBI nr
Match: KAG7025155.1 (DNA primase small subunit [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 166.0 bits (419), Expect = 2.4e-37
Identity = 76/84 (90.48%), Postives = 80/84 (95.24%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           K+QGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+RCDEFDP
Sbjct: 353 KSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPERCDEFDP 412

Query: 103 TTVPTVAQLLEELNAGDMIVDGET 127
           TTVPTVAQLLEELNA DM VD ET
Sbjct: 413 TTVPTVAQLLEELNAADMEVDSET 436

[Close]

BLAST of Spg004546.1 vs. ExPASy Swiss-Prot
Match: Q24317 (DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 3.9e-19
Identity = 47/94 (50.00%), Postives = 60/94 (63.83%), Query Frame = 0


Query: 27  ESVPKFERS-DTNLMEPKAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKT 86
           ES  K+  S  T+     ++ ++  V EI     YPRLD  VT+  NHLLKAPFC+HP T
Sbjct: 296 ESFIKYANSMRTSTTSAWSRKLKNIVAEIQLGLLYPRLDINVTRGFNHLLKAPFCIHPAT 355

Query: 87  GRVCVPINPKRCDEFDPTTVPTVAQLLEELNAGD 120
           G+VCVP +     +FDPTTVPT+ QLL E+NA D
Sbjct: 356 GKVCVPFSVSAVAKFDPTTVPTITQLLHEINAFD 389

[Close]

BLAST of Spg004546.1 vs. ExPASy Swiss-Prot
Match: O14215 (DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pri1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.0e-18
Identity = 40/65 (61.54%), Postives = 53/65 (81.54%), Query Frame = 0


Query: 52  EEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDPTTVPTVAQL 111
           ++IV ++ YPRLD EV++H+NHLLK+PFCVHP T RVCVPI+ +R D F+P  VPTV  L
Sbjct: 321 QDIVLTYLYPRLDVEVSRHLNHLLKSPFCVHPGTSRVCVPIDIERMDSFNPLKVPTVNDL 380

Query: 112 LEELN 117
           L+EL+
Sbjct: 381 LQELD 385

[Close]

BLAST of Spg004546.1 vs. ExPASy Swiss-Prot
Match: Q7KQM1 (DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF14_0366 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 6.3e-17
Identity = 39/70 (55.71%), Postives = 52/70 (74.29%), Query Frame = 0


Query: 47  MRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDPTTVP 106
           M   V+EIVF FTYPRLD  V+K +NHLLK+PFC+H  TGRVCVP++ K  + F+P +VP
Sbjct: 330 MPSFVKEIVFHFTYPRLDINVSKEINHLLKSPFCIHNSTGRVCVPLDIKNINNFNPQSVP 389

Query: 107 TVAQLLEELN 117
           T+  L E+ +
Sbjct: 390 TLKLLREQFD 399

[Close]

BLAST of Spg004546.1 vs. ExPASy Swiss-Prot
Match: Q25998 (DNA primase small subunit OS=Plasmodium falciparum (isolate K1 / Thailand) OX=5839 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 6.3e-17
Identity = 39/70 (55.71%), Postives = 52/70 (74.29%), Query Frame = 0


Query: 47  MRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDPTTVP 106
           M   V+EIVF FTYPRLD  V+K +NHLLK+PFC+H  TGRVCVP++ K  + F+P +VP
Sbjct: 330 MPSFVKEIVFHFTYPRLDINVSKEINHLLKSPFCIHNSTGRVCVPLDIKNINNFNPQSVP 389

Query: 107 TVAQLLEELN 117
           T+  L E+ +
Sbjct: 390 TLKLLREQFD 399

[Close]

BLAST of Spg004546.1 vs. ExPASy Swiss-Prot
Match: P49642 (DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-15
Identity = 36/65 (55.38%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 53  EIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDPTTVPTVAQLL 112
           EI+  + +PRLD  V+K +NHLLK+PF VHPKTGR+ VPI+ ++ D+FDP TVPT++ + 
Sbjct: 295 EIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFIC 354

Query: 113 EELNA 118
            EL+A
Sbjct: 355 RELDA 359

[Close]

BLAST of Spg004546.1 vs. ExPASy TrEMBL
Match: A0A5A7T8Y3 (DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 4.6e-39
Identity = 79/90 (87.78%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           KAQGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+ CDEFDP
Sbjct: 335 KAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPEHCDEFDP 394

Query: 103 TTVPTVAQLLEELNAGDMIVDGETGSKLCR 133
           TTVPTVAQLLEELNA DM VD ETG K+ R
Sbjct: 395 TTVPTVAQLLEELNAADMEVDSETGLKIKR 424

[Close]

BLAST of Spg004546.1 vs. ExPASy TrEMBL
Match: A0A6J1I8T4 (DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.1e-37
Identity = 76/84 (90.48%), Postives = 80/84 (95.24%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           K+QGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+RCDEFDP
Sbjct: 315 KSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPERCDEFDP 374

Query: 103 TTVPTVAQLLEELNAGDMIVDGET 127
           TTVPTVAQLLEELNA DM VD ET
Sbjct: 375 TTVPTVAQLLEELNAADMEVDSET 398

[Close]

BLAST of Spg004546.1 vs. ExPASy TrEMBL
Match: A0A0A0KUP7 (DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.5e-37
Identity = 76/83 (91.57%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGR+CVPI+P+ CDEFDP
Sbjct: 320 KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRICVPIDPEHCDEFDP 379

Query: 103 TTVPTVAQLLEELNAGDMIVDGE 126
           TTVPTVAQLLEELNA DM VD E
Sbjct: 380 TTVPTVAQLLEELNAADMEVDSE 402

[Close]

BLAST of Spg004546.1 vs. ExPASy TrEMBL
Match: A0A6J1CKZ3 (DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 4.4e-37
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           KAQGMRRHVEEIVFSFTYPRLD EVTKH NHLLKAPFCVHPKTGR+CVPINP+ CDEFDP
Sbjct: 320 KAQGMRRHVEEIVFSFTYPRLDLEVTKHTNHLLKAPFCVHPKTGRICVPINPENCDEFDP 379

Query: 103 TTVPTVAQLLEELNAGDMIVDGE 126
           TTVPT+AQLLEELNA DM VDGE
Sbjct: 380 TTVPTLAQLLEELNAADMNVDGE 402

[Close]

BLAST of Spg004546.1 vs. ExPASy TrEMBL
Match: A0A6J1EEZ9 (DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 5.7e-37
Identity = 75/84 (89.29%), Postives = 79/84 (94.05%), Query Frame = 0


Query: 43  KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPKRCDEFDP 102
           K+ GMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+P+RCDEFDP
Sbjct: 320 KSPGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPERCDEFDP 379

Query: 103 TTVPTVAQLLEELNAGDMIVDGET 127
           TTVPTVAQLLEELNA DM VD ET
Sbjct: 380 TTVPTVAQLLEELNAADMEVDSET 403

[Close]

BLAST of Spg004546.1 vs. TAIR 10
Match: AT5G41880.1 (DNA primases;DNA primases )

HSP 1 Score: 131.3 bits (329), Expect = 6.0e-31
Identity = 62/108 (57.41%), Postives = 79/108 (73.15%), Query Frame = 0


Query: 18  SRDFLGRHGESVPKFERSDTNLMEP--KAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLL 77
           +R  L     S+ ++E+    L     KA  +R  VEEIVF+FTYPR+D EV+K MNHLL
Sbjct: 293 ARSSLSEEATSLLRWEQLKKALQSKRNKALSLRTCVEEIVFTFTYPRIDLEVSKQMNHLL 352

Query: 78  KAPFCVHPKTGRVCVPINPKRCDEFDPTTVPTVAQLLEELNAGDMIVD 124
           KAPFCVHPKTGRVCVPI+P  CDEFDP  VPT++QL+EE+N+G + +D
Sbjct: 353 KAPFCVHPKTGRVCVPIDPNNCDEFDPLAVPTLSQLIEEINSGGLRMD 400

The following BLAST results are available for this feature:

BLAST of Spg004546.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039338.1	9.6e-39	87.78	DNA primase, small subunit [Cucumis melo var. makuwa] >TYK00521.1 DNA primase, s...	[more]
XP_022973496.1	2.4e-37	90.48	DNA primase small subunit [Cucurbita maxima]	[more]
XP_023535939.1	2.4e-37	90.48	DNA primase small subunit [Cucurbita pepo subsp. pepo]	[more]
KAG6592333.1	2.4e-37	90.48	DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7025155.1	2.4e-37	90.48	DNA primase small subunit [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Spg004546.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q24317	3.9e-19	50.00	DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2	[more]
O14215	2.0e-18	61.54	DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)...	[more]
Q7KQM1	6.3e-17	55.71	DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF1...	[more]
Q25998	6.3e-17	55.71	DNA primase small subunit OS=Plasmodium falciparum (isolate K1 / Thailand) OX=58...	[more]
P49642	1.2e-15	55.38	DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1	[more]

BLAST of Spg004546.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T8Y3	4.6e-39	87.78	DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 P...	[more]
A0A6J1I8T4	1.1e-37	90.48	DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1	[more]
A0A0A0KUP7	1.5e-37	91.57	DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1	[more]
A0A6J1CKZ3	4.4e-37	90.36	DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1	[more]
A0A6J1EEZ9	5.7e-37	89.29	DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1	[more]

BLAST of Spg004546.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41880.1	6.0e-31	57.41	DNA primases;DNA primases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002755	DNA primase, small subunit	PFAM	PF01896	DNA_primase_S	coord: 32..93 e-value: 3.4E-15 score: 56.5
None	No IPR available	GENE3D	3.90.920.10	DNA primase, PRIM domain	coord: 8..138 e-value: 1.9E-34 score: 121.4
None	No IPR available	PANTHER	PTHR10536:SF1	DNA PRIMASE	coord: 41..123
None	No IPR available	SUPERFAMILY	56747	Prim-pol domain	coord: 22..117
IPR014052	DNA primase, small subunit, eukaryotic/archaeal	PANTHER	PTHR10536	DNA PRIMASE SMALL SUBUNIT	coord: 41..123

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg004546	Spg004546	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg004546.1-cds	Spg004546.1-cds-scaffold9:9455803..9455855	CDS
Spg004546.1-cds	Spg004546.1-cds-scaffold9:9456123..9456171	CDS
Spg004546.1-cds	Spg004546.1-cds-scaffold9:9456565..9456638	CDS
Spg004546.1-cds	Spg004546.1-cds-scaffold9:9456861..9456918	CDS
Spg004546.1-cds	Spg004546.1-cds-scaffold9:9457118..9457186	CDS
Spg004546.1-cds	Spg004546.1-cds-scaffold9:9459578..9459706	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg004546.1	Spg004546.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006269	DNA replication, synthesis of RNA primer
biological_process	GO:0006865	amino acid transport
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0005658	alpha DNA polymerase:primase complex
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003896	DNA primase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0015293	symporter activity